Moc09g07450 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g07450
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionE3 ubiquitin-protein ligase MIEL1-like
Locationchr9: 5899534 .. 5899863 (+)
RNA-Seq ExpressionMoc09g07450
SyntenyMoc09g07450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCGATCAAATCCCTAGTACTTCCGATACCCCTACTCGCTCTTCTCCTGCTCTCCCAATCCCTAGCCGCCGATCACGGCGGGGATTCGAATTCCTCCACCGGAATGCTTCGCCCGAACAACGTCCTTCAGACCGCTGCGGAGGACCATCGGCTTCCAAAGAGCGACGGCGAGCTCTCCTTCGAGCGAAAAATCAGAAGGCGGACCCGACGATCCGTAGTAAGAGCCGATGGTCCGAGCCCGTCGGCGGCGAATCGGGGCCCAACGTCGTCGTTTGGCGTTGGATCTCTACTCTGTCTCTGTATTGTTCTTGGTTTGTTCGTGTGA

mRNA sequence

ATGGCTGCGATCAAATCCCTAGTACTTCCGATACCCCTACTCGCTCTTCTCCTGCTCTCCCAATCCCTAGCCGCCGATCACGGCGGGGATTCGAATTCCTCCACCGGAATGCTTCGCCCGAACAACGTCCTTCAGACCGCTGCGGAGGACCATCGGCTTCCAAAGAGCGACGGCGAGCTCTCCTTCGAGCGAAAAATCAGAAGGCGGACCCGACGATCCGTAGTAAGAGCCGATGGTCCGAGCCCGTCGGCGGCGAATCGGGGCCCAACGTCGTCGTTTGGCGTTGGATCTCTACTCTGTCTCTGTATTGTTCTTGGTTTGTTCGTGTGA

Coding sequence (CDS)

ATGGCTGCGATCAAATCCCTAGTACTTCCGATACCCCTACTCGCTCTTCTCCTGCTCTCCCAATCCCTAGCCGCCGATCACGGCGGGGATTCGAATTCCTCCACCGGAATGCTTCGCCCGAACAACGTCCTTCAGACCGCTGCGGAGGACCATCGGCTTCCAAAGAGCGACGGCGAGCTCTCCTTCGAGCGAAAAATCAGAAGGCGGACCCGACGATCCGTAGTAAGAGCCGATGGTCCGAGCCCGTCGGCGGCGAATCGGGGCCCAACGTCGTCGTTTGGCGTTGGATCTCTACTCTGTCTCTGTATTGTTCTTGGTTTGTTCGTGTGA

Protein sequence

MAAIKSLVLPIPLLALLLLSQSLAADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKSDGELSFERKIRRRTRRSVVRADGPSPSAANRGPTSSFGVGSLLCLCIVLGLFV
Homology
BLAST of Moc09g07450 vs. NCBI nr
Match: XP_022992473.1 (uncharacterized protein LOC111488793 isoform X1 [Cucurbita maxima])

HSP 1 Score: 92.0 bits (227), Expect = 3.3e-15
Identity = 65/114 (57.02%), Postives = 80/114 (70.18%), Query Frame = 0

Query: 1   MAAIKSLVLPIPLLALLLLSQSL---AADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKS- 60
           MA IKSL+  I LLALLLL + L   AADHG   +   G+ R  + LQT AE H++ ++ 
Sbjct: 63  MAPIKSLI-SIALLALLLLFERLPISAADHGVAPSPLAGVFR-LDTLQT-AERHQVAENG 122

Query: 61  -DGELSFERKIRRRTRRSVVRADGPSPSAANRGPTSSFGVGSLLCLCIVLGLFV 110
              + SFE+KIRRR RR+V RA+GPSPS+AN   TSSFGVGSLLCLC V GLF+
Sbjct: 123 IGNQYSFEQKIRRRARRAVRRAEGPSPSSANWSRTSSFGVGSLLCLCTVFGLFL 173

BLAST of Moc09g07450 vs. NCBI nr
Match: XP_022954099.1 (uncharacterized protein LOC111456466 [Cucurbita moschata])

HSP 1 Score: 91.7 bits (226), Expect = 4.3e-15
Identity = 65/114 (57.02%), Postives = 80/114 (70.18%), Query Frame = 0

Query: 1   MAAIKSLVLPIPLLALLLLSQSL---AADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKS- 60
           MA IKSL+  I LLALLLLS+ L   AADHG   +    + R  + LQT AE H++ ++ 
Sbjct: 90  MAPIKSLI-SIALLALLLLSEHLPISAADHGVAPSPLARVFR-RDTLQT-AERHQVAENG 149

Query: 61  -DGELSFERKIRRRTRRSVVRADGPSPSAANRGPTSSFGVGSLLCLCIVLGLFV 110
              + SFE+KIRRR RR+V RA+GPSPS+AN   TSSFGVGSLLCLC V GLF+
Sbjct: 150 IGYQYSFEQKIRRRARRAVRRAEGPSPSSANWSQTSSFGVGSLLCLCTVFGLFL 200

BLAST of Moc09g07450 vs. NCBI nr
Match: KAG6575858.1 (hypothetical protein SDJN03_26497, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 87.4 bits (215), Expect = 8.2e-14
Identity = 61/107 (57.01%), Postives = 76/107 (71.03%), Query Frame = 0

Query: 1   MAAIKSLVLPIPLLALLLLSQSL---AADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKS- 60
           MA IKSL+  I LLALLLLS+ L   AADHG   +   G+ R  + LQT AE H++ ++ 
Sbjct: 1   MAPIKSLI-SIALLALLLLSEHLPISAADHGVAPSPLAGVFR-RDTLQT-AERHQVAENG 60

Query: 61  -DGELSFERKIRRRTRRSVVRADGPSPSAANRGPTSSFGVGSLLCLC 103
              + SFE+KIRRR RR+V RA+GPSPS+AN   TSSFG+GSLLCLC
Sbjct: 61  IGYQYSFEQKIRRRARRAVRRAEGPSPSSANWSRTSSFGIGSLLCLC 104

BLAST of Moc09g07450 vs. NCBI nr
Match: KAG6575859.1 (hypothetical protein SDJN03_26498, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014393.1 hypothetical protein SDJN02_24570, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 72.4 bits (176), Expect = 2.7e-09
Identity = 60/113 (53.10%), Postives = 73/113 (64.60%), Query Frame = 0

Query: 1   MAAIKSLVLPIPLLALLLLSQSL---AADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKSD 60
           MAA+KSLV  + LLALLL S+SL   A D  GDSNS TGMLR  NVLQTAA  H++  + 
Sbjct: 1   MAALKSLV-SVALLALLLFSESLTASAVDRSGDSNSLTGMLR-RNVLQTAA--HQIADNS 60

Query: 61  GELSFERKIRRRTRRSV-VRADGPSPSAANRGPTSSFGVGSLLCLCIVLGLFV 110
            E S ERK+    +R +   A G   SAANR   +S GVGS+L   +VLGLF+
Sbjct: 61  VESSPERKMNVHIKRRMRPVATGTRSSAANRSRIASIGVGSVL---VVLGLFL 106

BLAST of Moc09g07450 vs. NCBI nr
Match: KAG6593163.1 (hypothetical protein SDJN03_12639, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 67.8 bits (164), Expect = 6.7e-08
Identity = 52/112 (46.43%), Postives = 68/112 (60.71%), Query Frame = 0

Query: 1   MAAIKSLVLPIPLLALLLLSQSL---AADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKSD 60
           MAAIK+L L I L+ALLL  +SL   AA H G S S  G+LR  N++QT A+ H++    
Sbjct: 1   MAAIKAL-LSIALIALLLFFESLPTSAAVHRGASTSLAGILR-RNIVQTTADHHQV---- 60

Query: 61  GELSFERKIRRRTRRSVVRADGPSPSAANRGPTSSFGVGSLLCLCIVLGLFV 110
            E  F   I+RR R   V    P  SAANR   +  GVGS+LC+ ++LGLF+
Sbjct: 61  AEKKFNVHIKRRMRMRPVLIGRPKTSAANRSREAFCGVGSMLCIFVLLGLFL 106

BLAST of Moc09g07450 vs. ExPASy TrEMBL
Match: A0A6J1JZB1 (uncharacterized protein LOC111488793 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488793 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.6e-15
Identity = 65/114 (57.02%), Postives = 80/114 (70.18%), Query Frame = 0

Query: 1   MAAIKSLVLPIPLLALLLLSQSL---AADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKS- 60
           MA IKSL+  I LLALLLL + L   AADHG   +   G+ R  + LQT AE H++ ++ 
Sbjct: 63  MAPIKSLI-SIALLALLLLFERLPISAADHGVAPSPLAGVFR-LDTLQT-AERHQVAENG 122

Query: 61  -DGELSFERKIRRRTRRSVVRADGPSPSAANRGPTSSFGVGSLLCLCIVLGLFV 110
              + SFE+KIRRR RR+V RA+GPSPS+AN   TSSFGVGSLLCLC V GLF+
Sbjct: 123 IGNQYSFEQKIRRRARRAVRRAEGPSPSSANWSRTSSFGVGSLLCLCTVFGLFL 173

BLAST of Moc09g07450 vs. ExPASy TrEMBL
Match: A0A6J1GRX2 (uncharacterized protein LOC111456466 OS=Cucurbita moschata OX=3662 GN=LOC111456466 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.1e-15
Identity = 65/114 (57.02%), Postives = 80/114 (70.18%), Query Frame = 0

Query: 1   MAAIKSLVLPIPLLALLLLSQSL---AADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKS- 60
           MA IKSL+  I LLALLLLS+ L   AADHG   +    + R  + LQT AE H++ ++ 
Sbjct: 90  MAPIKSLI-SIALLALLLLSEHLPISAADHGVAPSPLARVFR-RDTLQT-AERHQVAENG 149

Query: 61  -DGELSFERKIRRRTRRSVVRADGPSPSAANRGPTSSFGVGSLLCLCIVLGLFV 110
              + SFE+KIRRR RR+V RA+GPSPS+AN   TSSFGVGSLLCLC V GLF+
Sbjct: 150 IGYQYSFEQKIRRRARRAVRRAEGPSPSSANWSQTSSFGVGSLLCLCTVFGLFL 200

BLAST of Moc09g07450 vs. ExPASy TrEMBL
Match: A0A5D3D3Y6 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001920 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.7e-04
Identity = 50/118 (42.37%), Postives = 63/118 (53.39%), Query Frame = 0

Query: 1   MAAIKSLVLPIPLLALLLLSQSLAADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKSDGEL 60
           MAAI+ LV  I LLALL  S+SL   H     S TG +   N+L T A  HR+     E 
Sbjct: 1   MAAIRPLV-SIALLALLFFSESLLTSH-----SLTG-IPHRNILHTVA--HRIVNGSVES 60

Query: 61  SFERKI-------RRRTRRSVVRADG--PSPSAANRGPTSSFGVGSLLCLCIVLGLFV 110
           S++RK        RRR R  ++         SA NR   +SF VGS+LC+CI LGLF+
Sbjct: 61  SYQRKSNKLHTKDRRRIRPILISGSNGMHHKSAENRSRVASFEVGSMLCICIFLGLFL 109

BLAST of Moc09g07450 vs. ExPASy TrEMBL
Match: A0A5A7VLE5 (Protein no-on-transient A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00010 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.7e-04
Identity = 52/106 (49.06%), Postives = 62/106 (58.49%), Query Frame = 0

Query: 1   MAAIKSLVLPIPLLALLLLSQSL---AADHGGDSNSSTGMLRPNNVLQTAAEDHRLPKSD 60
           MAAIKSL+  I LLALL  S+SL   AAD  G SNS TGMLR  NVLQTA   H +  S+
Sbjct: 1   MAAIKSLLF-ISLLALLFFSESLSTSAADCRGASNSVTGMLR-RNVLQTAT--HEIADSE 60

Query: 61  GELSFERKIRRRTRRSVVRA----DGPSPSAANRGPTSSFGVGSLL 100
           G+++   K     R   V A     G   SAA+R   +SFG+G LL
Sbjct: 61  GKMNVHIKRGGGGRIRPVAAGTGFTGNRKSAASRSQVASFGLGFLL 102

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022992473.13.3e-1557.02uncharacterized protein LOC111488793 isoform X1 [Cucurbita maxima][more]
XP_022954099.14.3e-1557.02uncharacterized protein LOC111456466 [Cucurbita moschata][more]
KAG6575858.18.2e-1457.01hypothetical protein SDJN03_26497, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6575859.12.7e-0953.10hypothetical protein SDJN03_26498, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6593163.16.7e-0846.43hypothetical protein SDJN03_12639, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1JZB11.6e-1557.02uncharacterized protein LOC111488793 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1GRX22.1e-1557.02uncharacterized protein LOC111456466 OS=Cucurbita moschata OX=3662 GN=LOC1114564... [more]
A0A5D3D3Y61.7e-0442.37E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A5A7VLE51.7e-0449.06Protein no-on-transient A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..42
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..50

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g07450.1Moc09g07450.1mRNA