Moc09g06270 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc09g06270 vs. NCBI nr
Match: XP_022155218.1 (beta-carotene isomerase D27, chloroplastic [Momordica charantia])

HSP 1 Score: 539.7 bits (1389), Expect = 1.5e-149
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 1   MKPLAVLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGILD 60
           MKPLAVLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGILD
Sbjct: 1   MKPLAVLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGILD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPLL 120
           DFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPLL
Sbjct: 61  DFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCKY 180
           LELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCKY
Sbjct: 121 LELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCKY 180

Query: 181 LEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILKE 240
           LEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILKE
Sbjct: 181 LEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILKE 240

Query: 241 PCLEICPNATRRREVSGKMSAMQCPKA 268
           PCLEICPNATRRREVSGKMSAMQCPKA
Sbjct: 241 PCLEICPNATRRREVSGKMSAMQCPKA 267

BLAST of Moc09g06270 vs. NCBI nr
Match: XP_038898256.1 (beta-carotene isomerase D27, chloroplastic [Benincasa hispida])

HSP 1 Score: 471.9 bits (1213), Expect = 3.8e-129
Identity = 230/267 (86.14%), Postives = 248/267 (92.88%), Query Frame = 0

Query: 1   MKPLAVLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGILD 60
           MKPLA+LRPP ISS PLRF+ SKSHLRNH +N SCSALQSES IK+  S K EYKPG+LD
Sbjct: 1   MKPLALLRPPTISSAPLRFTFSKSHLRNHPLNLSCSALQSES-IKNETSSKPEYKPGLLD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPLL 120
           +FFLN+FRSKMVQEVGWDS+KPGY GL+EVANRLT KGKTNSDT+EASVRILT+LFPPLL
Sbjct: 61  EFFLNVFRSKMVQEVGWDSEKPGYDGLIEVANRLTTKGKTNSDTIEASVRILTALFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCKY 180
           L+LYR+LVSPIAGGKVAAIMVARVTALTCQWLMGT TVNS ELPDGSSCQSG+FVEKCKY
Sbjct: 121 LDLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSFELPDGSSCQSGVFVEKCKY 180

Query: 181 LEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILKE 240
           LEESKCIGICINTCKLPTQ+FFKD+MG+PLLMEPNF DYSCQFKFGVLP  PEED ILKE
Sbjct: 181 LEESKCIGICINTCKLPTQSFFKDRMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDGILKE 240

Query: 241 PCLEICPNATRRREVSGKMSAMQCPKA 268
           PCLEICPNATRRRE+SGKMSA QCPKA
Sbjct: 241 PCLEICPNATRRREISGKMSATQCPKA 266

BLAST of Moc09g06270 vs. NCBI nr
Match: XP_008451473.1 (PREDICTED: beta-carotene isomerase D27, chloroplastic [Cucumis melo])

HSP 1 Score: 468.4 bits (1204), Expect = 4.2e-128
Identity = 233/268 (86.94%), Postives = 249/268 (92.91%), Query Frame = 0

Query: 1   MKPLA-VLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGIL 60
           MKPLA +L PP ISS PLRF+SSKSHLRN     SCSALQS S IKDG S K+EYKPGIL
Sbjct: 1   MKPLALLLHPPTISSAPLRFNSSKSHLRNRPFILSCSALQSGS-IKDGTSSKSEYKPGIL 60

Query: 61  DDFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPL 120
           DDFFLN+FRSKMVQEVGWDS+KPGYGGL+EVA+RLTM G+TNS+T+EASVRILT+LFPPL
Sbjct: 61  DDFFLNVFRSKMVQEVGWDSEKPGYGGLIEVASRLTMTGRTNSETIEASVRILTALFPPL 120

Query: 121 LLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCK 180
           LL+LYR+LVSPIAGGKVAAIMVARVTALTCQWLMGT TVNSIELPDGSSCQSG+FVEKCK
Sbjct: 121 LLKLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180

Query: 181 YLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILK 240
           YLEESKCIGICINTCKLPTQ+FFKDQMG+PLLMEPNF DYSCQFKFGVLP  PEEDSILK
Sbjct: 181 YLEESKCIGICINTCKLPTQSFFKDQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK 240

Query: 241 EPCLEICPNATRRREVSGKMSAMQCPKA 268
           EPCLEICPNATRRREVSGKMSA QCPKA
Sbjct: 241 EPCLEICPNATRRREVSGKMSAAQCPKA 267

BLAST of Moc09g06270 vs. NCBI nr
Match: KAA0046701.1 (beta-carotene isomerase D27 [Cucumis melo var. makuwa] >TYK18237.1 beta-carotene isomerase D27 [Cucumis melo var. makuwa])

HSP 1 Score: 467.6 bits (1202), Expect = 7.1e-128
Identity = 232/268 (86.57%), Postives = 249/268 (92.91%), Query Frame = 0

Query: 1   MKPLA-VLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGIL 60
           MKP+A +L PP ISS PLRF+SSKSHLRN     SCSALQS S IKDG S K+EYKPGIL
Sbjct: 1   MKPMALLLHPPTISSAPLRFNSSKSHLRNRPFILSCSALQSGS-IKDGTSSKSEYKPGIL 60

Query: 61  DDFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPL 120
           DDFFLN+FRSKMVQEVGWDS+KPGYGGL+EVA+RLTM G+TNS+T+EASVRILT+LFPPL
Sbjct: 61  DDFFLNVFRSKMVQEVGWDSEKPGYGGLIEVASRLTMTGRTNSETIEASVRILTALFPPL 120

Query: 121 LLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCK 180
           LL+LYR+LVSPIAGGKVAAIMVARVTALTCQWLMGT TVNSIELPDGSSCQSG+FVEKCK
Sbjct: 121 LLKLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180

Query: 181 YLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILK 240
           YLEESKCIGICINTCKLPTQ+FFKDQMG+PLLMEPNF DYSCQFKFGVLP  PEEDSILK
Sbjct: 181 YLEESKCIGICINTCKLPTQSFFKDQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK 240

Query: 241 EPCLEICPNATRRREVSGKMSAMQCPKA 268
           EPCLEICPNATRRREVSGKMSA QCPKA
Sbjct: 241 EPCLEICPNATRRREVSGKMSAAQCPKA 267

BLAST of Moc09g06270 vs. NCBI nr
Match: KGN45022.1 (hypothetical protein Csa_016689 [Cucumis sativus])

HSP 1 Score: 461.5 bits (1186), Expect = 5.1e-126
Identity = 230/268 (85.82%), Postives = 246/268 (91.79%), Query Frame = 0

Query: 1   MKPLA-VLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGIL 60
           MKP+A +L PP IS  PLRF+SSKSHLRN     SCSALQS S IKDG S K EYKPGIL
Sbjct: 1   MKPVALLLHPPTISPAPLRFTSSKSHLRNRPFILSCSALQSGS-IKDGASSKAEYKPGIL 60

Query: 61  DDFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPL 120
           DDFFLN+FRSKMVQEVGWDS+KPGY GL+EVA+RLTM GKTNS+T+EASVRIL +LFPPL
Sbjct: 61  DDFFLNVFRSKMVQEVGWDSEKPGYDGLIEVASRLTMTGKTNSETIEASVRILIALFPPL 120

Query: 121 LLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCK 180
           LL+LYR+LVSPIAGGKVAAIMVARVTALTCQWLMGT TVNSIELPDGSSCQSG+FVEKCK
Sbjct: 121 LLKLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180

Query: 181 YLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILK 240
           YLEESKCIGICINTCKLPTQ+FFKDQMG+PLLMEPNFTDYSCQFKFGVLP  PEEDSILK
Sbjct: 181 YLEESKCIGICINTCKLPTQSFFKDQMGIPLLMEPNFTDYSCQFKFGVLPPLPEEDSILK 240

Query: 241 EPCLEICPNATRRREVSGKMSAMQCPKA 268
           EPCLEICPNATRRREVSGK+SA QCPKA
Sbjct: 241 EPCLEICPNATRRREVSGKISAAQCPKA 267

BLAST of Moc09g06270 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.8e-26
Identity = 68/198 (34.34%), Postives = 111/198 (56.06%), Query Frame = 0

Query: 58  ILDDFFLNL---FRSKMVQE---VGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRI 117
           I D FF  +   + SK +Q+   +   S    Y  L++ A R++     N DT +    +
Sbjct: 65  IEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVS----RNFDTKQQHEFV 124

Query: 118 LTSL---FPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSS 177
           L+SL    P ++  L +M   P    KV+  + A  T ++  WL+G S V   E+ +G  
Sbjct: 125 LSSLDRALPTVISSLIKMAFPP---SKVSRELFALFTTISFAWLVGPSEVRETEV-NGRK 184

Query: 178 CQSGIFVEKCKYLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVL 237
            +S +++EKC++LE+S C+G+C + CK+P+Q F K+ +G+P+ MEP+F D SC+  FG  
Sbjct: 185 EKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGRE 244

Query: 238 PSPPEEDSILKEPCLEIC 247
           P   E+D  +K+PC E C
Sbjct: 245 PPEIEDDPAMKQPCFEFC 254

BLAST of Moc09g06270 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.8e-26
Identity = 67/218 (30.73%), Postives = 109/218 (50.00%), Query Frame = 0

Query: 29  HSINTSCSALQSESIIKDGVSPKTEYKPGILDDFFLNLFRSKMVQEVGWDSDKPGYGGLM 88
           H+      A  ++ I ++ +     YK    D   +N     +    G  ++K G+  L+
Sbjct: 28  HTFVVGVLARPTDDISEETLRKTNVYKDNWFDKLAINHLSKSVQAATGISNNKSGFDSLV 87

Query: 89  EVANRLTMKGKTNSDTVEASVRILTSLFPPLLLELYRMLVSPIAGGKVAAIMVARVTALT 148
           E A   + K  T        +  L   FP  +L + R ++ P    K+A    A  T + 
Sbjct: 88  EAATVASQKFNTTQQQ-GIILDALDRAFPKPILSVIRRVMPP---SKLAREYFAVFTTIF 147

Query: 149 CQWLMGTSTVNSIELPDGSSCQSGIFVEKCKYLEESKCIGICINTCKLPTQNFFKDQMGV 208
             WL+G S V   E+ +G   ++ + ++KC++LEE+ C+G+CIN CK+P+Q F KD  G+
Sbjct: 148 FAWLLGPSEVRESEI-NGRREKNIVHIKKCRFLEETNCVGMCINLCKMPSQLFIKDSFGM 207

Query: 209 PLLMEPNFTDYSCQFKFGVLPSPPEEDSILKEPCLEIC 247
           P+ M PNF D SC+  FG  P    +D  LK+PC ++C
Sbjct: 208 PVNMVPNFDDMSCEMIFGQEPPASTDDPALKQPCYKLC 240

BLAST of Moc09g06270 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.2e-22
Identity = 61/210 (29.05%), Postives = 106/210 (50.48%), Query Frame = 0

Query: 36  SALQSESIIKDGVSPKTEYKPGILDDFFLNLFRSKMVQEVGWDSDKPGYGGLMEVA---N 95
           S +Q+E+       P T Y+    D   +      + +  G  ++K GY  L++ A   +
Sbjct: 65  STVQTETA---AAPPATVYRDSWFDKLAIGYLSRNLQEASGLKNEKDGYESLIDAALAIS 124

Query: 96  RLTMKGKTNSDTVEASVRILTSLFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQWL 155
           R+    K +    +A  R L    P  +L + ++++ P    + +    A  T +   WL
Sbjct: 125 RIFSLDKQSEIVTQALERAL----PSYILTMIKVMMPP---SRFSREYFAAFTTIFFPWL 184

Query: 156 MGTSTVNSIELPDGSSCQSGIFVEKCKYLEESKCIGICINTCKLPTQNFFKDQMGVPLLM 215
           +G   V   E+ +G   ++ +++ KC++LE + C+G+C N CK+P Q F +D +G+ + M
Sbjct: 185 VGPCEVMESEV-EGRKEKNVVYIPKCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVYM 244

Query: 216 EPNFTDYSCQFKFGVLPSPPEEDSILKEPC 243
            PNF D SC+  FG    PPE+D  LK+PC
Sbjct: 245 SPNFEDMSCEMIFG--QQPPEDDPALKQPC 261

BLAST of Moc09g06270 vs. ExPASy TrEMBL
Match: A0A6J1DMD9 (beta-carotene isomerase D27, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111022359 PE=4 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 7.1e-150
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 1   MKPLAVLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGILD 60
           MKPLAVLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGILD
Sbjct: 1   MKPLAVLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGILD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPLL 120
           DFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPLL
Sbjct: 61  DFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCKY 180
           LELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCKY
Sbjct: 121 LELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCKY 180

Query: 181 LEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILKE 240
           LEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILKE
Sbjct: 181 LEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILKE 240

Query: 241 PCLEICPNATRRREVSGKMSAMQCPKA 268
           PCLEICPNATRRREVSGKMSAMQCPKA
Sbjct: 241 PCLEICPNATRRREVSGKMSAMQCPKA 267

BLAST of Moc09g06270 vs. ExPASy TrEMBL
Match: A0A1S3BRJ4 (beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492751 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.0e-128
Identity = 233/268 (86.94%), Postives = 249/268 (92.91%), Query Frame = 0

Query: 1   MKPLA-VLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGIL 60
           MKPLA +L PP ISS PLRF+SSKSHLRN     SCSALQS S IKDG S K+EYKPGIL
Sbjct: 1   MKPLALLLHPPTISSAPLRFNSSKSHLRNRPFILSCSALQSGS-IKDGTSSKSEYKPGIL 60

Query: 61  DDFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPL 120
           DDFFLN+FRSKMVQEVGWDS+KPGYGGL+EVA+RLTM G+TNS+T+EASVRILT+LFPPL
Sbjct: 61  DDFFLNVFRSKMVQEVGWDSEKPGYGGLIEVASRLTMTGRTNSETIEASVRILTALFPPL 120

Query: 121 LLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCK 180
           LL+LYR+LVSPIAGGKVAAIMVARVTALTCQWLMGT TVNSIELPDGSSCQSG+FVEKCK
Sbjct: 121 LLKLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180

Query: 181 YLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILK 240
           YLEESKCIGICINTCKLPTQ+FFKDQMG+PLLMEPNF DYSCQFKFGVLP  PEEDSILK
Sbjct: 181 YLEESKCIGICINTCKLPTQSFFKDQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK 240

Query: 241 EPCLEICPNATRRREVSGKMSAMQCPKA 268
           EPCLEICPNATRRREVSGKMSA QCPKA
Sbjct: 241 EPCLEICPNATRRREVSGKMSAAQCPKA 267

BLAST of Moc09g06270 vs. ExPASy TrEMBL
Match: A0A5D3D2R7 (Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001770 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.4e-128
Identity = 232/268 (86.57%), Postives = 249/268 (92.91%), Query Frame = 0

Query: 1   MKPLA-VLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGIL 60
           MKP+A +L PP ISS PLRF+SSKSHLRN     SCSALQS S IKDG S K+EYKPGIL
Sbjct: 1   MKPMALLLHPPTISSAPLRFNSSKSHLRNRPFILSCSALQSGS-IKDGTSSKSEYKPGIL 60

Query: 61  DDFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPL 120
           DDFFLN+FRSKMVQEVGWDS+KPGYGGL+EVA+RLTM G+TNS+T+EASVRILT+LFPPL
Sbjct: 61  DDFFLNVFRSKMVQEVGWDSEKPGYGGLIEVASRLTMTGRTNSETIEASVRILTALFPPL 120

Query: 121 LLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCK 180
           LL+LYR+LVSPIAGGKVAAIMVARVTALTCQWLMGT TVNSIELPDGSSCQSG+FVEKCK
Sbjct: 121 LLKLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180

Query: 181 YLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILK 240
           YLEESKCIGICINTCKLPTQ+FFKDQMG+PLLMEPNF DYSCQFKFGVLP  PEEDSILK
Sbjct: 181 YLEESKCIGICINTCKLPTQSFFKDQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK 240

Query: 241 EPCLEICPNATRRREVSGKMSAMQCPKA 268
           EPCLEICPNATRRREVSGKMSA QCPKA
Sbjct: 241 EPCLEICPNATRRREVSGKMSAAQCPKA 267

BLAST of Moc09g06270 vs. ExPASy TrEMBL
Match: A0A0A0K9J2 (DUF4033 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407750 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.5e-126
Identity = 230/268 (85.82%), Postives = 246/268 (91.79%), Query Frame = 0

Query: 1   MKPLA-VLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGIL 60
           MKP+A +L PP IS  PLRF+SSKSHLRN     SCSALQS S IKDG S K EYKPGIL
Sbjct: 1   MKPVALLLHPPTISPAPLRFTSSKSHLRNRPFILSCSALQSGS-IKDGASSKAEYKPGIL 60

Query: 61  DDFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPL 120
           DDFFLN+FRSKMVQEVGWDS+KPGY GL+EVA+RLTM GKTNS+T+EASVRIL +LFPPL
Sbjct: 61  DDFFLNVFRSKMVQEVGWDSEKPGYDGLIEVASRLTMTGKTNSETIEASVRILIALFPPL 120

Query: 121 LLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCK 180
           LL+LYR+LVSPIAGGKVAAIMVARVTALTCQWLMGT TVNSIELPDGSSCQSG+FVEKCK
Sbjct: 121 LLKLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180

Query: 181 YLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILK 240
           YLEESKCIGICINTCKLPTQ+FFKDQMG+PLLMEPNFTDYSCQFKFGVLP  PEEDSILK
Sbjct: 181 YLEESKCIGICINTCKLPTQSFFKDQMGIPLLMEPNFTDYSCQFKFGVLPPLPEEDSILK 240

Query: 241 EPCLEICPNATRRREVSGKMSAMQCPKA 268
           EPCLEICPNATRRREVSGK+SA QCPKA
Sbjct: 241 EPCLEICPNATRRREVSGKISAAQCPKA 267

BLAST of Moc09g06270 vs. ExPASy TrEMBL
Match: A0A6J1H551 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460668 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.6e-120
Identity = 225/269 (83.64%), Postives = 240/269 (89.22%), Query Frame = 0

Query: 1   MKPLA-VLRPPA-ISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGI 60
           MKP+  +L PPA ISS  LRF SSKSHLR   +N SC A+QSES  K  VS K EYKPG+
Sbjct: 1   MKPVVLLLHPPATISSAALRF-SSKSHLRKRPLNISCFAVQSES-TKVEVSSKPEYKPGM 60

Query: 61  LDDFFLNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPP 120
           LDDFFLNLFRSKMVQEVGWDS+KPGY GL+EVANRLTMKGK+NSDT+EASVRIL +LFPP
Sbjct: 61  LDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPP 120

Query: 121 LLLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKC 180
           LLLELYRMLVSPIAGGKVAAIMVARVTA TCQWLMGT TVNSIELPDGSSCQSG+FVEKC
Sbjct: 121 LLLELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKC 180

Query: 181 KYLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSIL 240
           KYLEESKCIGIC+NTCK PTQ+FFK+QMG+PLLMEPNF DYSCQFKFGVLP  PEEDSIL
Sbjct: 181 KYLEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSIL 240

Query: 241 KEPCLEICPNATRRREVSGKMSAMQCPKA 268
           KEPCLEICPNATRRREV  KMSA QCPKA
Sbjct: 241 KEPCLEICPNATRRREVLNKMSATQCPKA 267

BLAST of Moc09g06270 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 307.8 bits (787), Expect = 8.8e-84
Identity = 156/264 (59.09%), Postives = 194/264 (73.48%), Query Frame = 0

Query: 4   LAVLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESIIKDGVSPKTEYKPGILDDFF 63
           + VL PP  +   + FS+S S     +    C    S  +  D  +PK EYKPG LDDFF
Sbjct: 5   IQVLLPPPTT---IVFSNSFSTKLILTRRLRCRISNSSEVKSDEGAPKLEYKPGPLDDFF 64

Query: 64  LNLFRSKMVQEVGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRILTSLFPPLLLEL 123
           +  FR+K+V+EVG DS+KPGY GL+E+   L +KG+T S+T +A+VRIL SLFPPL+LEL
Sbjct: 65  MQSFRNKLVEEVGSDSEKPGYVGLIELVKLLLLKGRTRSETSDAAVRILKSLFPPLILEL 124

Query: 124 YRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSSCQSGIFVEKCKYLEE 183
           Y++L++PIA GK+AA+MVARVT LTCQWLMG S VN I+LP+G S  SG+FVEKC+YLEE
Sbjct: 125 YKLLIAPIAQGKLAALMVARVTVLTCQWLMGPSKVNIIDLPNGESWDSGVFVEKCQYLEE 184

Query: 184 SKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVLPSPPEEDSILKEPCL 243
           SKC+G+CINTCKLPTQ FFKD MGVPL+MEPNF DYSCQFKFGV  +PPE+D  + EPC 
Sbjct: 185 SKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKDYSCQFKFGV--APPEDDGNVNEPCF 244

Query: 244 EICPNATRRREVSGKMSAMQCPKA 268
           E C  A RR+  SG     +CP A
Sbjct: 245 ETCSIAGRRKLKSG-----ECPLA 258

BLAST of Moc09g06270 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 138.7 bits (348), Expect = 7.0e-33
Identity = 82/223 (36.77%), Postives = 124/223 (55.61%), Query Frame = 0

Query: 51  KTEYKPGILDDFFLNLFRSKM----------VQEVG-WDSDKPGYGGLMEVANRLTMKGK 110
           KT Y+ G+++  F+ LF  KM           +E G W+ D   Y   +EV+ R+ M+G+
Sbjct: 36  KTRYEDGLVERVFMGLFARKMDKFGSKKKKDTKEKGFWEYD---YESFVEVSKRV-MQGR 95

Query: 111 TNSDTVEASVRILTSLFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVN 170
           +     EA   +L S+ PP   E +R L  P    K AA   A +T     WL+G S V 
Sbjct: 96  SRVQQQEAVREVLLSMLPPGAPEQFRKLFPPT---KWAAEFNAALTVPFFHWLVGPSQVI 155

Query: 171 SIELPDGSSCQSGIFVEKCKYLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDY 230
            +E+ +G   +SG+ ++KC+YLE S C+G+C+N CK+PTQ+FF ++ G+PL M PN+ D 
Sbjct: 156 EVEV-NGVKQRSGVRIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNYEDM 215

Query: 231 SCQFKFGVLPSPPEEDSILKEPCL-EICPNATRRREVSGKMSA 262
           SC+  +G  P   EED   K+PCL +IC  +     +  K+ A
Sbjct: 216 SCEMIYGQAPPAFEEDVATKQPCLADICSMSNPSSPICPKLEA 250

BLAST of Moc09g06270 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 120.6 bits (301), Expect = 2.0e-27
Identity = 68/198 (34.34%), Postives = 111/198 (56.06%), Query Frame = 0

Query: 58  ILDDFFLNL---FRSKMVQE---VGWDSDKPGYGGLMEVANRLTMKGKTNSDTVEASVRI 117
           I D FF  +   + SK +Q+   +   S    Y  L++ A R++     N DT +    +
Sbjct: 65  IEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVS----RNFDTKQQHEFV 124

Query: 118 LTSL---FPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTSTVNSIELPDGSS 177
           L+SL    P ++  L +M   P    KV+  + A  T ++  WL+G S V   E+ +G  
Sbjct: 125 LSSLDRALPTVISSLIKMAFPP---SKVSRELFALFTTISFAWLVGPSEVRETEV-NGRK 184

Query: 178 CQSGIFVEKCKYLEESKCIGICINTCKLPTQNFFKDQMGVPLLMEPNFTDYSCQFKFGVL 237
            +S +++EKC++LE+S C+G+C + CK+P+Q F K+ +G+P+ MEP+F D SC+  FG  
Sbjct: 185 EKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGRE 244

Query: 238 PSPPEEDSILKEPCLEIC 247
           P   E+D  +K+PC E C
Sbjct: 245 PPEIEDDPAMKQPCFEFC 254