Moc09g06010 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g06010
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectin lyase-like superfamily protein
Locationchr9: 4646950 .. 4648768 (+)
RNA-Seq ExpressionMoc09g06010
SyntenyMoc09g06010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAGTTTTGTGTTGTCACTTGCATTTTGGTGTTCTTTTATTTGTTGCCTTGTTTCTTCTCAAGGTAGATGGCATCATCCCCCCAAGCAAAAGCACTCTCACCACCACAAACATAGCTACATTTCACAGCCACCATCTCCTCCTCCTCCTCCTCCTGATTCACCGGACGAGGGAGATTACGTTAGCGAGATGGATGTTTTCGACGTGAGAGCTTTCGGTGCTATCGGTGACGGCACTGCAGATGACACGGCTGCATTTAAGATGGCATGGGACACGGCCTGCCAAAGCGACGACCCTGCAGTAATTCTCGTTCCCTATAGTTTCTCCTTTATGATTCAATCAACGATCTTCACAGGCCCTTGTCAGGGTCACCTTATGTTACGAGTCGATGGCACATTGATGCCACCAGATGGGCCCGATGCCTGGCCAAAAAATACGAGTCATCGACAATGGTTGGTATTTTATAGAATTGATGGAATGTCCCTTGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAGCCACACAAGGTACTCGAAGGTCTTTTTACCATGCGTAGGTAAATGTTTAGAGAATATAAATGAACAATAATTCATGGATATATTATTCTAACTCTCTTAATTTTCATCACGTTAGGGAATAAATGGAACTACGCTACCAGGACCATGTGATAGCCCAATTGTAAGTTCAAGAACCTTACAAAAGTGTTTGCTGATATATAATACTTTTCTGTAAGAAATATGAAGATAAACTTAGACATTGTCACATTGTTATGGTTTAGGCTATAAGGTTCTTCATGAGCTCCAATTTGACTGTGCAAGGACTTAAAATCACAAACAGCCCTCAGTTCCACTTCAGATTCGACGGTTGCAAGAACGTTCATATCGACTCGATTCACATAAAATCTCCCGGTCTTAGTCCGAATACTGATGGTATCCACATCGAGAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGTCAGTATTGAAACCACAAAAATCTTTTACATTGTTAAGGGAATTATATATATATTCTGATTCATCTCTTTTATGCAGGGGATGATTGTGTATCAATTGGGTCAGGTTCATATGATGTAGATATCAGAAACATCACATGTGGACCAAGTCATGGAATCAGGTATTATACGTTTTATTAGTAGTTCTAAAATATAATTACGACCATAATCTGAATATAATTTAACTAGTTAAGATACATATATACTAAGTTTCATGTTAATTAATATTGCTTATAAACCTTTTATCCTTCTTTTGGTGTAGCATTGGAAGTCTTGGCGTTCGCAACTCGAGAGCCTGTGTTACGAACATAACGGTTAGAGACTCCGTGATCAAAGTATCAGACAACGGAGTTCGGATCAAGACATGGCAAGGTGGCTATGGGGCAGTAAGAGGAATAACATTTAGCAACGTTCACATGGACAATGTTAGGAACCCCATCATTATAGACCAATTTTATTGTCTATCAAAAGATTGCACCAACCAAACCTCAGCTGTTTCAGTATCAGACATTCAGTACTCAAACATCAAAGGAACATACGACATTCGAAGCCCGCCGATGCACCTTGCCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGCTGTCGGAGATCGAGTTGCTTCCTGCTCAGGGAGATATAGTGTTGGACCCTTATTGTTGGAATGCCTATGGAGATCTTCAGACGCTTACTATTCCACCCATCTCTTGCTTGCAAGAGGGGCTTCCTTCTTCTATCAACTTAAACAATGACAATGAGTATTGTTGA

mRNA sequence

ATGGCTAGTTTTGTGTTGTCACTTGCATTTTGGTGTTCTTTTATTTGTTGCCTTGTTTCTTCTCAAGGTAGATGGCATCATCCCCCCAAGCAAAAGCACTCTCACCACCACAAACATAGCTACATTTCACAGCCACCATCTCCTCCTCCTCCTCCTCCTGATTCACCGGACGAGGGAGATTACGTTAGCGAGATGGATGTTTTCGACGTGAGAGCTTTCGGTGCTATCGGTGACGGCACTGCAGATGACACGGCTGCATTTAAGATGGCATGGGACACGGCCTGCCAAAGCGACGACCCTGCAGTAATTCTCGTTCCCTATAGTTTCTCCTTTATGATTCAATCAACGATCTTCACAGGCCCTTGTCAGGGTCACCTTATGTTACGAGTCGATGGCACATTGATGCCACCAGATGGGCCCGATGCCTGGCCAAAAAATACGAGTCATCGACAATGGTTGGTATTTTATAGAATTGATGGAATGTCCCTTGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAGCCACACAAGGGAATAAATGGAACTACGCTACCAGGACCATGTGATAGCCCAATTGCTATAAGGTTCTTCATGAGCTCCAATTTGACTGTGCAAGGACTTAAAATCACAAACAGCCCTCAGTTCCACTTCAGATTCGACGGTTGCAAGAACGTTCATATCGACTCGATTCACATAAAATCTCCCGGTCTTAGTCCGAATACTGATGGTATCCACATCGAGAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGGGATGATTGTGTATCAATTGGGTCAGGTTCATATGATGTAGATATCAGAAACATCACATGTGGACCAAGTCATGGAATCAGCATTGGAAGTCTTGGCGTTCGCAACTCGAGAGCCTGTGTTACGAACATAACGGTTAGAGACTCCGTGATCAAAGTATCAGACAACGGAGTTCGGATCAAGACATGGCAAGGTGGCTATGGGGCAGTAAGAGGAATAACATTTAGCAACGTTCACATGGACAATGTTAGGAACCCCATCATTATAGACCAATTTTATTGTCTATCAAAAGATTGCACCAACCAAACCTCAGCTGTTTCAGTATCAGACATTCAGTACTCAAACATCAAAGGAACATACGACATTCGAAGCCCGCCGATGCACCTTGCCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGCTGTCGGAGATCGAGTTGCTTCCTGCTCAGGGAGATATAGTGTTGGACCCTTATTGTTGGAATGCCTATGGAGATCTTCAGACGCTTACTATTCCACCCATCTCTTGCTTGCAAGAGGGGCTTCCTTCTTCTATCAACTTAAACAATGACAATGAGTATTGTTGA

Coding sequence (CDS)

ATGGCTAGTTTTGTGTTGTCACTTGCATTTTGGTGTTCTTTTATTTGTTGCCTTGTTTCTTCTCAAGGTAGATGGCATCATCCCCCCAAGCAAAAGCACTCTCACCACCACAAACATAGCTACATTTCACAGCCACCATCTCCTCCTCCTCCTCCTCCTGATTCACCGGACGAGGGAGATTACGTTAGCGAGATGGATGTTTTCGACGTGAGAGCTTTCGGTGCTATCGGTGACGGCACTGCAGATGACACGGCTGCATTTAAGATGGCATGGGACACGGCCTGCCAAAGCGACGACCCTGCAGTAATTCTCGTTCCCTATAGTTTCTCCTTTATGATTCAATCAACGATCTTCACAGGCCCTTGTCAGGGTCACCTTATGTTACGAGTCGATGGCACATTGATGCCACCAGATGGGCCCGATGCCTGGCCAAAAAATACGAGTCATCGACAATGGTTGGTATTTTATAGAATTGATGGAATGTCCCTTGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAGCCACACAAGGGAATAAATGGAACTACGCTACCAGGACCATGTGATAGCCCAATTGCTATAAGGTTCTTCATGAGCTCCAATTTGACTGTGCAAGGACTTAAAATCACAAACAGCCCTCAGTTCCACTTCAGATTCGACGGTTGCAAGAACGTTCATATCGACTCGATTCACATAAAATCTCCCGGTCTTAGTCCGAATACTGATGGTATCCACATCGAGAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGGGATGATTGTGTATCAATTGGGTCAGGTTCATATGATGTAGATATCAGAAACATCACATGTGGACCAAGTCATGGAATCAGCATTGGAAGTCTTGGCGTTCGCAACTCGAGAGCCTGTGTTACGAACATAACGGTTAGAGACTCCGTGATCAAAGTATCAGACAACGGAGTTCGGATCAAGACATGGCAAGGTGGCTATGGGGCAGTAAGAGGAATAACATTTAGCAACGTTCACATGGACAATGTTAGGAACCCCATCATTATAGACCAATTTTATTGTCTATCAAAAGATTGCACCAACCAAACCTCAGCTGTTTCAGTATCAGACATTCAGTACTCAAACATCAAAGGAACATACGACATTCGAAGCCCGCCGATGCACCTTGCCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGCTGTCGGAGATCGAGTTGCTTCCTGCTCAGGGAGATATAGTGTTGGACCCTTATTGTTGGAATGCCTATGGAGATCTTCAGACGCTTACTATTCCACCCATCTCTTGCTTGCAAGAGGGGCTTCCTTCTTCTATCAACTTAAACAATGACAATGAGTATTGTTGA

Protein sequence

MASFVLSLAFWCSFICCLVSSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC
Homology
BLAST of Moc09g06010 vs. NCBI nr
Match: XP_022146163.1 (polygalacturonase At1g48100 [Momordica charantia])

HSP 1 Score: 981.5 bits (2536), Expect = 2.5e-282
Identity = 464/465 (99.78%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 1   MASFVLSLAFWCSFICCLVSSQGRWHHPPKQKHSHHHKHSYISQPPS-PPPPPPDSPDEG 60
           MASFVLSLAFWCSFICCLVSSQGRWHHPPKQKHSHHHKHSYISQPPS PPPPPPDSPDEG
Sbjct: 1   MASFVLSLAFWCSFICCLVSSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPPDSPDEG 60

Query: 61  DYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFT 120
           DYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFT
Sbjct: 61  DYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFT 120

Query: 121 GPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL 180
           GPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL
Sbjct: 121 GPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL 180

Query: 181 PCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIH 240
           PCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIH
Sbjct: 181 PCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIH 240

Query: 241 IKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300
           IKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG
Sbjct: 241 IKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300

Query: 301 SLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIID 360
           SLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIID
Sbjct: 301 SLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIID 360

Query: 361 QFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPA 420
           QFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPA
Sbjct: 361 QFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPA 420

Query: 421 QGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           QGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC
Sbjct: 421 QGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465

BLAST of Moc09g06010 vs. NCBI nr
Match: XP_038899064.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 892.1 bits (2304), Expect = 2.0e-255
Identity = 422/469 (89.98%), Postives = 440/469 (93.82%), Query Frame = 0

Query: 1   MASFVLSLAFWCSFICCLV--SSQGRWHHPPKQKHSHHHKHSYISQPP--SPPPPPPDS- 60
           M+SFVLS A W SFI CL+  SSQ RWHH  KQKHSHHHKHSYIS PP  SP P P DS 
Sbjct: 1   MSSFVLSFAIWFSFISCLLSSSSQARWHHHTKQKHSHHHKHSYISHPPSLSPSPSPSDSP 60

Query: 61  PDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQS 120
           PD+GDY++E ++FDVRAFGAIG+G  DDT AFKMAWDTACQSDD A++LVPY +SFMIQS
Sbjct: 61  PDDGDYINETNIFDVRAFGAIGNGVVDDTEAFKMAWDTACQSDDSAIMLVPYGYSFMIQS 120

Query: 121 TIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQK 180
           TIFTGPC GHLMLRVDGTLMPPDGPDAWPKN S RQWLVFYRIDGMSLEGGGVIDGRGQK
Sbjct: 121 TIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQK 180

Query: 181 WWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHI 240
           WWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHI
Sbjct: 181 WWDLPCKPHKGRNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHI 240

Query: 241 DSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHG 300
           DSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYD+DIRNITCGPSHG
Sbjct: 241 DSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDLDIRNITCGPSHG 300

Query: 301 ISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNP 360
           ISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNP
Sbjct: 301 ISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNP 360

Query: 361 IIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIE 420
           IIIDQFYCL+KDC NQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTLSEIE
Sbjct: 361 IIIDQFYCLTKDCINQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIE 420

Query: 421 LLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           LLP+QGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSINLNNDNEYC
Sbjct: 421 LLPSQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 469

BLAST of Moc09g06010 vs. NCBI nr
Match: TYJ96898.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 882.1 bits (2278), Expect = 2.1e-252
Identity = 418/467 (89.51%), Postives = 437/467 (93.58%), Query Frame = 0

Query: 1   MASFV-LSLAFWCSFICCLV-SSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPDSPDE 60
           M+SFV LS A W SF+ CL+ SSQ RWHH  KQKHSHHH+HSYIS PPS  P P DSPD+
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSFMIQSTI 120
           GDY+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD AV+LVPY FSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPC G+LMLRVDGTLMPPDGPDAWPKN+S RQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELL 420
           IDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           PAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSIN NNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Moc09g06010 vs. NCBI nr
Match: XP_008461394.1 (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo])

HSP 1 Score: 880.6 bits (2274), Expect = 6.1e-252
Identity = 417/467 (89.29%), Postives = 436/467 (93.36%), Query Frame = 0

Query: 1   MASFV-LSLAFWCSFICCLV-SSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPDSPDE 60
           M+SFV LS A W SF+ CL+ SSQ RWHH  KQKHSHHH+H YIS PPS  P P DSPD+
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPDD 60

Query: 61  GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSFMIQSTI 120
           GDY+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD AV+LVPY FSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPC G+LMLRVDGTLMPPDGPDAWPKN+S RQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELL 420
           IDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           PAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSIN NNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Moc09g06010 vs. NCBI nr
Match: XP_022992012.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 878.2 bits (2268), Expect = 3.0e-251
Identity = 408/465 (87.74%), Postives = 435/465 (93.55%), Query Frame = 0

Query: 1   MASFVLSLAFWCSFICCLV-SSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPDSPDEG 60
           M++FVLSL+FW +F+CCL+ SSQ RWHH  KQKHSHHHK SYIS PP+P P    SP +G
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSP----SPGDG 60

Query: 61  DYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFT 120
           DY++E ++FDVR FGA+GDG ADDT AFKMAWDTACQSDD AV+LVPY +SFMIQSTIFT
Sbjct: 61  DYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIFT 120

Query: 121 GPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL 180
           GPC GHLML VDGTLMPPDGPDAWP+N S RQWLVFYRIDGMSLEGGGV+DGRGQKWWDL
Sbjct: 121 GPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDL 180

Query: 181 PCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIH 240
           PCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIH
Sbjct: 181 PCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIH 240

Query: 241 IKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300
           IKSPGLSPNTDGIHIENTN V IYNS+IYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG
Sbjct: 241 IKSPGLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300

Query: 301 SLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIID 360
           SLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNPIIID
Sbjct: 301 SLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIID 360

Query: 361 QFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPA 420
           QFYCL+KDCTNQTSAVS+SDI+Y+NIKGTYDIRS PMHLACSD+VPCTNLTLSEIELLPA
Sbjct: 361 QFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPA 420

Query: 421 QGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           QGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSI LNNDNEYC
Sbjct: 421 QGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Moc09g06010 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.2e-157
Identity = 259/458 (56.55%), Postives = 333/458 (72.71%), Query Frame = 0

Query: 23  GRWHHPPKQKHSHHHKHSYISQPPSPPPPPP---------------DSPDEGDYVSEMD- 82
           G + +  + ++ +H     ++ P  PPPPPP                SPD     S+   
Sbjct: 19  GHFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGC 78

Query: 83  VFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHL 142
           VFDV +FGA+GDG+ DDTAAF+ AW  AC + +  V+L P    F I STIF+GPC+  L
Sbjct: 79  VFDVTSFGAVGDGSCDDTAAFQDAWKAAC-AVESGVVLAPEGGVFKITSTIFSGPCKPGL 138

Query: 143 MLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 202
           + ++DG LMPPDGP+ WP+  +  QWLVFYR+DG +  G G ++G GQKWWDLPCKPH+G
Sbjct: 139 VFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRG 198

Query: 203 INGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 262
            +G++  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V I+ I I SP LS
Sbjct: 199 PDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLS 258

Query: 263 PNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 322
           PNTDGIH+ NT  V IYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS
Sbjct: 259 PNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNS 318

Query: 323 RACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIIDQFYCLSK 382
           +ACV+NITVR++VI+ SDNG+R+KTWQGG G+V  + F N+ M+NV N II+DQ+YC SK
Sbjct: 319 QACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSK 378

Query: 383 DCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLD 442
           DC N+TSAV V D++Y NIKGTYD+RSPP+H ACSD+V CTN+T+SE+ELLP +G++V D
Sbjct: 379 DCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDD 438

Query: 443 PYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           P+CWNAYG  +TLTIPPI CL +G P      + N  C
Sbjct: 439 PFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of Moc09g06010 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 4.3e-91
Identity = 165/379 (43.54%), Postives = 231/379 (60.95%), Query Frame = 0

Query: 73  FGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHLMLRVDG 132
           FGA G+G  DDT AF  AW TAC S     ILVP +++ +++    +GPC+  L L++ G
Sbjct: 58  FGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARLTLQISG 117

Query: 133 TLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGINGTTL 192
           T++ P+ PD W +  + R+WL F+ +  +++EGGG ++G GQ+WW+  CK H   N    
Sbjct: 118 TIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK-HNHSN---- 177

Query: 193 PGPC-DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDG 252
             PC  +P A+ F    N+ V+ L + +S Q H     C+ V I  + + +P  SPNTDG
Sbjct: 178 --PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDG 237

Query: 253 IHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVT 312
           IHI  + G+ I N+ +  GDDC+SI   S  + I NI CGP HGISIGSLG   S   V 
Sbjct: 238 IHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVR 297

Query: 313 NITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIIDQFYCLS-KDCTN 372
           +ITV  ++I  + NGVRIKTWQGG G V  I F N+ M+NV NPIIIDQ+YC S K C N
Sbjct: 298 DITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCAN 357

Query: 373 QTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLDPYCW 432
           QTSA+S+ +I + +++GT       + ++CSDS PC N+ L +I+L P+ GD   + +CW
Sbjct: 358 QTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCW 417

Query: 433 NAYGDLQTLTIPPISCLQE 450
            AYG       PP  CL +
Sbjct: 418 EAYGSSSGQVYPP-PCLSD 426

BLAST of Moc09g06010 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.0e-84
Identity = 161/360 (44.72%), Postives = 218/360 (60.56%), Query Frame = 0

Query: 65  MDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQG 124
           +++F+V  +GA+GDG  D T AF  AW  AC+    A++LVP +  F++ +  F GPCQ 
Sbjct: 56  INIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPS-AMLLVPGNKKFVVNNLFFNGPCQP 115

Query: 125 HLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPH 184
           H   +VDG +     P +W  N   R WL F ++ G +L G GVIDG+G++WW   C   
Sbjct: 116 HFTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQC--- 175

Query: 185 KGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPG 244
           K +NG  +    D P AI+F  S+ L +QGLK+ NSP+FH  F  C+ V I  I I +P 
Sbjct: 176 KWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPR 235

Query: 245 LSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVR 304
            SPNTDGI I  +    +  + I  GDDCV+IG+GS ++ I ++ CGP HGISIGSLG  
Sbjct: 236 DSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRE 295

Query: 305 NSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIIDQFYCL 364
           NSRA V+ + V  +    + NG+RIKTWQGG G    I + NV M N  NPI+I+QFYC 
Sbjct: 296 NSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCT 355

Query: 365 SKD-CTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDI 424
           S   C NQ SAV + D+ Y NI+GT    +  + L CSDS+PC ++ LS+I L    G I
Sbjct: 356 SASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

BLAST of Moc09g06010 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 4.3e-83
Identity = 162/356 (45.51%), Postives = 218/356 (61.24%), Query Frame = 0

Query: 67  VFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHL 126
           VF+V  +GA+GDG  D T AF   W+ AC+    AV+LVP +  F + + +F GPCQ HL
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKAS-AVLLVPANKKFFVNNLVFRGPCQPHL 117

Query: 127 MLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 186
             +VDGT++    P  W KN+  + WL F ++   +L G GVIDG+GQ+WW   CK    
Sbjct: 118 SFKVDGTIVAQPDPARW-KNS--KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV--- 177

Query: 187 INGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 246
           +NG T+    + P AI+   S ++TV+ L + NSP+FH  F  C+ V I  + IK+P  S
Sbjct: 178 VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDS 237

Query: 247 PNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 306
           PNTDGI I  +    I   VI  GDDC++IG+GS ++ I+++ CGP HGISIGSLG  NS
Sbjct: 238 PNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNS 297

Query: 307 RACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIIDQFYCLSK 366
           RA V+++ V  +    + NG+RIKTWQGG G    IT+ NV M N  NPI+I+QFYC S 
Sbjct: 298 RAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSA 357

Query: 367 D-CTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQG 422
             C NQ SAV +  + Y NI GT    +  + L CSDSVPCT + LS + L    G
Sbjct: 358 SACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSLKLTSG 405

BLAST of Moc09g06010 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 3.7e-82
Identity = 161/358 (44.97%), Postives = 217/358 (60.61%), Query Frame = 0

Query: 67  VFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHL 126
           VF+V  +GA+GDG  D T AF+  W+ AC     AV LVP +  F++ + +F GPCQ H 
Sbjct: 59  VFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLS-AVFLVPANKKFVVNNLVFYGPCQPHF 118

Query: 127 MLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 186
             +VDGT+     P  W KN+  + W+ F R+   +L G GVIDG+G +WW   CK    
Sbjct: 119 SFKVDGTIAAYPDPAKW-KNS--KIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKT--- 178

Query: 187 INGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 246
           ING T+      P AI+   S ++TV+ L +TNSP+FH  F  C  V I  I IK+P  S
Sbjct: 179 INGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDS 238

Query: 247 PNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 306
           PNTDGI I  +   EI    I  GDDCV++G+GS ++ I+++TCGP HG+SIGSLG  NS
Sbjct: 239 PNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNS 298

Query: 307 RACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIIDQFYCLS- 366
           R+ V+ + +  +    + NG+RIKTWQGG G    IT+ NV M N  NPI+I+QFYC S 
Sbjct: 299 RSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSA 358

Query: 367 KDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDI 424
             C NQ SAV + D+ + NI GT    +  + L CSDSVPC+N+ LS + L    G +
Sbjct: 359 AACKNQRSAVKIQDVTFKNIHGT-SATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKV 408

BLAST of Moc09g06010 vs. ExPASy TrEMBL
Match: A0A6J1CXU8 (polygalacturonase At1g48100 OS=Momordica charantia OX=3673 GN=LOC111015443 PE=3 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.2e-282
Identity = 464/465 (99.78%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 1   MASFVLSLAFWCSFICCLVSSQGRWHHPPKQKHSHHHKHSYISQPPS-PPPPPPDSPDEG 60
           MASFVLSLAFWCSFICCLVSSQGRWHHPPKQKHSHHHKHSYISQPPS PPPPPPDSPDEG
Sbjct: 1   MASFVLSLAFWCSFICCLVSSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPPDSPDEG 60

Query: 61  DYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFT 120
           DYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFT
Sbjct: 61  DYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFT 120

Query: 121 GPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL 180
           GPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL
Sbjct: 121 GPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL 180

Query: 181 PCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIH 240
           PCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIH
Sbjct: 181 PCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIH 240

Query: 241 IKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300
           IKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG
Sbjct: 241 IKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300

Query: 301 SLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIID 360
           SLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIID
Sbjct: 301 SLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIID 360

Query: 361 QFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPA 420
           QFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPA
Sbjct: 361 QFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPA 420

Query: 421 QGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           QGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC
Sbjct: 421 QGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465

BLAST of Moc09g06010 vs. ExPASy TrEMBL
Match: A0A5D3BFC7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00080 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.0e-252
Identity = 418/467 (89.51%), Postives = 437/467 (93.58%), Query Frame = 0

Query: 1   MASFV-LSLAFWCSFICCLV-SSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPDSPDE 60
           M+SFV LS A W SF+ CL+ SSQ RWHH  KQKHSHHH+HSYIS PPS  P P DSPD+
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSFMIQSTI 120
           GDY+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD AV+LVPY FSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPC G+LMLRVDGTLMPPDGPDAWPKN+S RQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELL 420
           IDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           PAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSIN NNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Moc09g06010 vs. ExPASy TrEMBL
Match: A0A1S3CFX8 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.0e-252
Identity = 417/467 (89.29%), Postives = 436/467 (93.36%), Query Frame = 0

Query: 1   MASFV-LSLAFWCSFICCLV-SSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPDSPDE 60
           M+SFV LS A W SF+ CL+ SSQ RWHH  KQKHSHHH+H YIS PPS  P P DSPD+
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPDD 60

Query: 61  GDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQS-DDPAVILVPYSFSFMIQSTI 120
           GDY+++ ++FDVRAFGAIGDG  DDT AFKMAWDTACQS DD AV+LVPY FSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPC G+LMLRVDGTLMPPDGPDAWPKN+S RQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELL 420
           IDQFYCL+KDCTNQTSAVSVSDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           PAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSIN NNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Moc09g06010 vs. ExPASy TrEMBL
Match: A0A6J1JNI5 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.5e-251
Identity = 408/465 (87.74%), Postives = 435/465 (93.55%), Query Frame = 0

Query: 1   MASFVLSLAFWCSFICCLV-SSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPDSPDEG 60
           M++FVLSL+FW +F+CCL+ SSQ RWHH  KQKHSHHHK SYIS PP+P P    SP +G
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSP----SPGDG 60

Query: 61  DYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFT 120
           DY++E ++FDVR FGA+GDG ADDT AFKMAWDTACQSDD AV+LVPY +SFMIQSTIFT
Sbjct: 61  DYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIFT 120

Query: 121 GPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL 180
           GPC GHLML VDGTLMPPDGPDAWP+N S RQWLVFYRIDGMSLEGGGV+DGRGQKWWDL
Sbjct: 121 GPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDL 180

Query: 181 PCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIH 240
           PCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIH
Sbjct: 181 PCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIH 240

Query: 241 IKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300
           IKSPGLSPNTDGIHIENTN V IYNS+IYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG
Sbjct: 241 IKSPGLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300

Query: 301 SLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIID 360
           SLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNPIIID
Sbjct: 301 SLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIID 360

Query: 361 QFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPA 420
           QFYCL+KDCTNQTSAVS+SDI+Y+NIKGTYDIRS PMHLACSD+VPCTNLTLSEIELLPA
Sbjct: 361 QFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPA 420

Query: 421 QGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           QGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSI LNNDNEYC
Sbjct: 421 QGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Moc09g06010 vs. ExPASy TrEMBL
Match: A0A6J1GP58 (polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.5e-251
Identity = 408/465 (87.74%), Postives = 434/465 (93.33%), Query Frame = 0

Query: 1   MASFVLSLAFWCSFICCLV-SSQGRWHHPPKQKHSHHHKHSYISQPPSPPPPPPDSPDEG 60
           M++FVLSL+FW +F+CCL+ SSQ RWHH  KQKHSHHHK SYIS PP+P P    SP +G
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSP----SPGDG 60

Query: 61  DYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFT 120
           DY++E ++FDVR FGA+GDG ADDT AFKMAWD ACQSDD AV+LVPY +SFMIQSTIFT
Sbjct: 61  DYINETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSFMIQSTIFT 120

Query: 121 GPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL 180
           GPC GHLML VDGTLMPPDGPDAWP+N S RQWLVFYRIDGMSLEGGGV+DGRGQKWWDL
Sbjct: 121 GPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDL 180

Query: 181 PCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIH 240
           PCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIH
Sbjct: 181 PCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIH 240

Query: 241 IKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300
           IKSPG SPNTDGIHIENTNGV IYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG
Sbjct: 241 IKSPGFSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIG 300

Query: 301 SLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIID 360
           SLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRGITFSN+HMDNVRNPIIID
Sbjct: 301 SLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIID 360

Query: 361 QFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPA 420
           QFYCL+KDCTNQTSAVS+SDI+Y+NIKGTYDIRS PMHLACSD+VPCTNLTLSEIELLPA
Sbjct: 361 QFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPA 420

Query: 421 QGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           QGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSI LNNDNEYC
Sbjct: 421 QGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Moc09g06010 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 645.6 bits (1664), Expect = 3.1e-185
Identity = 304/469 (64.82%), Postives = 371/469 (79.10%), Query Frame = 0

Query: 8   LAFWCSFICCLVSSQGR-WHHPPKQKHSHHHKH--------SYISQPPSPPPPPPDSPDE 67
           L F    +  +  S+ R  HH  K KH++H+ H        S ISQPP+PPP PPDSP  
Sbjct: 12  LGFMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAP 71

Query: 68  GDYVSEMD--------VFDVRAFGAIGDGTADDTAAFKMAWDTACQSDD--PAVILVPYS 127
               S  D        +++VR +GA+GDG  DDT AFK AWD++C +++   +V+LVPY 
Sbjct: 72  SLPPSPSDDPADDNNGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYG 131

Query: 128 FSFMIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGV 187
           ++FMIQSTIFTGPC+ +   +VDGT++ PDGP++WP N S RQWLVFYR++GM+L+G GV
Sbjct: 132 YTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGV 191

Query: 188 IDGRGQKWWDLPCKPHKGINGTTL-PGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRF 247
           IDGRGQKWWDLPCKPH+ +N + +  GPCDSPIA+RFFMSSNL V+GL+I NSPQFHFRF
Sbjct: 192 IDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRF 251

Query: 248 DGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRN 307
           DGC+ VH++S+HI +P LSPNTDGIHIEN+N V IYNS+I NGDDCVSIGSGSYDVDIRN
Sbjct: 252 DGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRN 311

Query: 308 ITCGP-SHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSN 367
           +TCGP  HGISIGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+TF+N
Sbjct: 312 LTCGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNN 371

Query: 368 VHMDNVRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPC 427
           +H+D+VRNPIIIDQ+YC++KDC N+TSAV VSDI Y  IKGTYDIRSPPMH  CSD+VPC
Sbjct: 372 IHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPC 431

Query: 428 TNLTLSEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSI 456
           TNLTLS+IELLPA+G+IVLDP+CWNAYG  + L+IPP+ CL    P  +
Sbjct: 432 TNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGL 480

BLAST of Moc09g06010 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 580.9 bits (1496), Expect = 9.3e-166
Identity = 271/425 (63.76%), Postives = 332/425 (78.12%), Query Frame = 0

Query: 39  HSYISQPPSPPPPPPD---SPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTAC 98
           H   SQPPSP P P D   SP     +    V+DVR +GA+G+G ADDT +FK AWD+AC
Sbjct: 33  HHQASQPPSPSPNPNDPSKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSAC 92

Query: 99  ---QSDDPAVILVPYSFSFMIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQW 158
              +++  +V+ VPY F+FMI+STIFTGPC+ +   +VDGT++P DGP +WP + + RQW
Sbjct: 93  SNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQW 152

Query: 159 LVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPH-KGINGTTLPGPCDSPIAIRFFMSSNLT 218
           L FYRI+GM+L+G GVIDGRGQ WWDLPCKPH + +N T L GPC+SP A+RFFMSSN+ 
Sbjct: 153 LAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVI 212

Query: 219 VQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGD 278
           V+GL I NSPQ H + DGC  VHI+S+ I SP  SPNTDGIHIEN+N VEIYNSVI NGD
Sbjct: 213 VKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVISNGD 272

Query: 279 DCVSIGSGSYDVDIRNITCGP-SHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIK 338
           DCVSIG G+YD+DIRNITCGP  HGISIGSLG +NS ACV+N+TVRDS IK S+NGVRIK
Sbjct: 273 DCVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIK 332

Query: 339 TWQGGYGAVRGITFSNVHMDNVRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYD 398
           TWQGG G+V G+TF N+H+D VRNPIIIDQ+YC +K C N+TSAV V+DI Y +IKGTYD
Sbjct: 333 TWQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYD 392

Query: 399 IRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEG 456
           IRSPPMH  CS++VPCTNLTLS IELLP++ DIV+ P+CWNAYG     ++P ISCL+  
Sbjct: 393 IRSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLKSN 452

BLAST of Moc09g06010 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 557.8 bits (1436), Expect = 8.4e-159
Identity = 259/458 (56.55%), Postives = 333/458 (72.71%), Query Frame = 0

Query: 23  GRWHHPPKQKHSHHHKHSYISQPPSPPPPPP---------------DSPDEGDYVSEMD- 82
           G + +  + ++ +H     ++ P  PPPPPP                SPD     S+   
Sbjct: 19  GHFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGC 78

Query: 83  VFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGPCQGHL 142
           VFDV +FGA+GDG+ DDTAAF+ AW  AC + +  V+L P    F I STIF+GPC+  L
Sbjct: 79  VFDVTSFGAVGDGSCDDTAAFQDAWKAAC-AVESGVVLAPEGGVFKITSTIFSGPCKPGL 138

Query: 143 MLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 202
           + ++DG LMPPDGP+ WP+  +  QWLVFYR+DG +  G G ++G GQKWWDLPCKPH+G
Sbjct: 139 VFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRG 198

Query: 203 INGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 262
            +G++  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V I+ I I SP LS
Sbjct: 199 PDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLS 258

Query: 263 PNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 322
           PNTDGIH+ NT  V IYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS
Sbjct: 259 PNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNS 318

Query: 323 RACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPIIIDQFYCLSK 382
           +ACV+NITVR++VI+ SDNG+R+KTWQGG G+V  + F N+ M+NV N II+DQ+YC SK
Sbjct: 319 QACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSK 378

Query: 383 DCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELLPAQGDIVLD 442
           DC N+TSAV V D++Y NIKGTYD+RSPP+H ACSD+V CTN+T+SE+ELLP +G++V D
Sbjct: 379 DCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDD 438

Query: 443 PYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465
           P+CWNAYG  +TLTIPPI CL +G P      + N  C
Sbjct: 439 PFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of Moc09g06010 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 551.2 bits (1419), Expect = 7.9e-157
Identity = 249/408 (61.03%), Postives = 326/408 (79.90%), Query Frame = 0

Query: 47  SPPPPPPDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDP--AVIL 106
           SP P P  + ++ D ++   VFDV +FGAIGD + DDT+AFKMAWD AC S  P  A++L
Sbjct: 28  SPSPAPNPAYNDNDNIAP-TVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLL 87

Query: 107 VPYSFSFMIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLE 166
           VPY+F F+++ T F GPC+ +L+L++DG ++ PDGP +WP N   RQW++FYR++G+S++
Sbjct: 88  VPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSN-YQRQWMMFYRVNGLSIQ 147

Query: 167 GGGVIDGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFH 226
           G GVI+GRGQKWW+LPCKPHKG+NGTT  GPCDSP+AIR F SS + +QG+   NS QFH
Sbjct: 148 GSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFH 207

Query: 227 FRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVD 286
            RFD C +V +DS+ IK+P  SPNTDGIHIENT+ V+I NS+I NGDDC+SIG+G ++VD
Sbjct: 208 VRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVD 267

Query: 287 IRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITF 346
           I+N+TCGPSHGISIGSLGV NS+A V+NITV +S I  SDNGVRIKTWQGG G+V  I F
Sbjct: 268 IKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVF 327

Query: 347 SNVHMDNVRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSV 406
           SN+ M NVRNPI+IDQ+YC + +C NQTSAV +SD+ Y+NIKGTYD+RSPP+H  CSDSV
Sbjct: 328 SNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSV 387

Query: 407 PCTNLTLSEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLP 453
           PCTNLTL+E++L P++G  + +P+CWNAYG ++ +T+PP+ CL +  P
Sbjct: 388 PCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of Moc09g06010 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 375.9 bits (964), Expect = 4.5e-104
Identity = 203/528 (38.45%), Postives = 301/528 (57.01%), Query Frame = 0

Query: 5   VLSLAFWCSFICCLVSSQGR-WHH---------------PPKQKHSHHHKHSY------- 64
           ++++  W + +   ++ +GR W H                PK   ++HH  S+       
Sbjct: 17  LVTILVWSATLETCIARRGRHWRHHHRSSSSLSDSLSSKKPKSHENNHHHMSHKSKPKPK 76

Query: 65  --------------ISQPP---SPPPP---PPDSPDEGDYVSEMDVFDVRAFGAIGDGTA 124
                         +SQPP    PPPP   PP +P     V +   F+V  FGA GDG +
Sbjct: 77  MKTQPPKSNDGSPVVSQPPQVQQPPPPHVQPPPTPLPLQPVEDSQQFNVLDFGAKGDGMS 136

Query: 125 DDTAAFKMAWDTACQSDDPAVILVPYSFSFMIQSTIFTGP-CQGHLMLRVDGTLMPPDGP 184
           DDT AF+ AW +AC+  + + +++P  + F++    F+GP CQ +++ +++G ++ P   
Sbjct: 137 DDTQAFEAAWASACKV-EASTMIIPPDYIFLVGPISFSGPYCQANIVFQLEGMIVAPTDT 196

Query: 185 DAWPKNTSHRQWLVFYRIDGMSLEGGGVIDGRGQKWW------DLPC------------- 244
           ++W        W+ F ++ G++++G GVIDGRG  WW      D P              
Sbjct: 197 ESW--GGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDDFKLIVPLNNS 256

Query: 245 ---KPHKGING-TTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 304
              +P   I        P   P A+RF+ S ++TV G+ I NSPQ H +FD C  V +  
Sbjct: 257 VQERPPMPIRSELNWRMPSIKPTALRFYGSIDVTVTGITIQNSPQCHLKFDNCVKVLVHD 316

Query: 305 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGIS 364
           +++ SPG SPNTDGIH++NT  V I+ + +  GDDC+SI +G  +V + N+ CGP HGIS
Sbjct: 317 VNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHNVNCGPGHGIS 376

Query: 365 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDNVRNPII 424
           IGSLG  +++ACV+NITVRD V+  +  GVRIKTWQGG G+V+GI FSN+ +  V+ PI+
Sbjct: 377 IGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNIQLTEVQLPIV 436

Query: 425 IDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTLSEIELL 465
           IDQFYC    C N TSAVSV  + Y  I+GTY ++  P+H ACSDS PC ++ LS IEL 
Sbjct: 437 IDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVK--PVHFACSDSFPCIDVQLSGIELK 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146163.12.5e-28299.78polygalacturonase At1g48100 [Momordica charantia][more]
XP_038899064.12.0e-25589.98polygalacturonase At1g48100-like [Benincasa hispida][more]
TYJ96898.12.1e-25289.51polygalacturonase [Cucumis melo var. makuwa][more]
XP_008461394.16.1e-25289.29PREDICTED: polygalacturonase At1g48100-like [Cucumis melo][more]
XP_022992012.13.0e-25187.74polygalacturonase At1g48100 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q949Z11.2e-15756.55Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ54.3e-9143.54Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432121.0e-8444.72Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E74.3e-8345.51Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9FY193.7e-8244.97Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CXU81.2e-28299.78polygalacturonase At1g48100 OS=Momordica charantia OX=3673 GN=LOC111015443 PE=3 ... [more]
A0A5D3BFC71.0e-25289.51Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00... [more]
A0A1S3CFX83.0e-25289.29polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV... [more]
A0A6J1JNI51.5e-25187.74polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=... [more]
A0A6J1GP582.5e-25187.74polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G02460.13.1e-18564.82Pectin lyase-like superfamily protein [more]
AT4G01890.19.3e-16663.76Pectin lyase-like superfamily protein [more]
AT1G48100.18.4e-15956.55Pectin lyase-like superfamily protein [more]
AT1G56710.17.9e-15761.03Pectin lyase-like superfamily protein [more]
AT1G60590.14.5e-10438.45Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 230..256
e-value: 94.0
score: 11.9
coord: 280..300
e-value: 6600.0
score: 1.4
coord: 257..278
e-value: 90.0
score: 11.9
coord: 310..331
e-value: 14.0
score: 14.6
coord: 339..360
e-value: 600.0
score: 8.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 61..453
e-value: 2.7E-120
score: 404.1
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 95..416
e-value: 2.3E-89
score: 299.7
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 287..300
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..61
NoneNo IPR availablePANTHERPTHR31375:SF119POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEINcoord: 31..453
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 31..453
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 66..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g06010.1Moc09g06010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity