Moc09g01520 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g01520
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectinesterase
Locationchr9: 1288632 .. 1292080 (+)
RNA-Seq ExpressionMoc09g01520
SyntenyMoc09g01520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAACCCATCGTCGCCGGAATGGAAATCTCAGATTCCGGTCACCGGAGCACTCCCGTCCCCCGAAACAGAAACAGCAAGAAGACGCTGCTTCTGGCTCTGTCCGCAGCTCTCCTCGTGGTCGGCGCCGTCGTGGGCATTGTCGCCGGCGTGACGAAGTCGAGAAACTCCGGCCGCCAGTCGGAAGAAGCCCACGCCATTGTGAAGAGCACGTGCAGCGCCACGCTGTACCCGGAGCTGTGCTTCTCGACGATTTCCCGGAGCGAGGGAGCGATGAAGAAAGTTTCGAATCAGAAGGACGTGATTGAGCTGTCGATCAACATAACGGTGAAGGCGGTGGAGGAGAATTACTTCAGAGTGCAGAAATTGGCGGCGCTGAAGAACATAACCCACCGCGAGAGAATTGCGCTCCACGATTGCCTTGAGACCATCGACGAGACGCTGGATGAGCTCCACAAGTCCATCGTGGACCTCCATCAGTACCCCAATAAGAAGTCTCTCGACCAGCACGCCGACGACCTCAAGACCCTGCTCAGTTCCGCCATTACCAATCAGGTTCCTTAATTTGTTACTCGACTCTAAAATTACAGCAGATTTAAGGTTAATTTGGTTCTATAATATGATTTAAGCTTAATTTTAAATTATAATTATCCTCTGTAGTTTTAAAATTTTCAATTTACTCTGGTAGAGATCAAGTTGGAGCCGATCCCAACTAGTTTAGACTAAGTTAACCTATTTTTGGAAGCATGGGAACCAAATTGAAAACAATGAATATTAGATTTGTAATTTAAATAAATCTCCTGAATTTTGTATATTTTTTACAGCATTTTAACTTTTTGAAAAATAAAAAGAATATATTTTATCTATTGCCTAAAAAAAAATTCCCTTTTGTTTGGATGGTAAGGCGCAACTATAAAATTTTTGGCTACTCCTTGAAGTAACTTTACACTTCCATTGAATATTTGAGAATTTATTATTTTTTAAAAGAAAATTTTCCTTCTTTATTTTATTTATTTTATTTTGTTAGTTGTCAAATATGTAATTTGCATTTCTATCTTAATTGTCTAATGAAAAATACTAAATCTTGTCTTCATCACCTGAAAAAATTTTATAAAAAATTTACATTTTTACATTTCTCTCTCTTATATGAGAAAGATGGTAGGGAGAAGATTGCGAAGTATTCTTCATGATAAGAATAAGGTTTGTTCATTTAACTATTTTCCTTTTCAATTTTTAAAAACAAAATATATGATGTTCTTGCTACTATTGAAATTTTGATAACTCATTGTTATTTTAAAAAATATATACTTTTACAATTATTTTTTTGTCGAATATGTAGCGGTTGGTGAAAATAAGATTAATGAGCAGCAAGTAGGATAAACTAGTATCAGAATAAAAAAAGATGAAATTAATATCAGTATAAGTCAGTTATAATTGACATCTATTTTTTACTTAAAAAAATCATTTCGAATTCTCGTATACAATTTAGCAGTGCTAAAGATCAAGCAGAATGATTAATTTTGTTTGAATTTGCAGGAAACTTGCTTGGATGGATTCTCACACGACGACGCGGATAAAAGCCTGCGGAAGAAGCTGGAAGAGGGGCAGAAGGAAGTGGAGCACATGTGCAGCAATGCACTCGCCATGATCAAGAACATGACCGATACCGATATTGCCAACTACCACGCCAAAATGGGAGCTAACAGAAACCTCATTGAGAACGCCGACGCCGGAGACGCCGCCATCATCTGGCCCCACTGGCTCTCGCCCGGAGACCGCCGCCTTCTGCAGTCCTCCTCCGTCACGCCGGACGCGGTCGTGGCGGCGGACGGCAGTGGGAACTTCCGGACGGTGTCTGCAGCCGTGTCCGCCGCGCCGGACAAAAGCAAGAAGAGGTACATTATTAGAATCAAAGCCGGCGTCTACAGGGAGAATGTGGAAATTCCCAAGAAGAAAACCAATATAATGTTTGTGGGAGATGGACGAAGCAACACCATTATCACCGGCAGCCGGAATGTCGTCGACGGCAGCACCACTTTCAACTCTGCAACCGTCGGTGAGTTCCCATAATTCTATGACTTTTACGATTTATATTTATTTATTTTATATCTAATAATAAGTCTCTAATAAAAAAATGTATAATAAATTAAAATAGATCATGTTCCTATCTTTTTTAAAGTGTTGCTATCTTATCTCGCCTCTTTTTCCTGCTATCCATCCGGTGCTCTGGTGGAGCGCACATGGGCAAAAGTTACCGCCGTACAAAAAGATTTAAACCCACTTCTAAAATCCCTTTATCTTCTCTCCTTTCGAGTGCACCTTCTCAAATTTTGAAGTACTGAATTGACTTGTGAAACATCGAATTTACTTTTTTTTTTCTACTCTATTTTCATGATAATATCTAAAAAAGAAAAAACCTATTTTATAATTAAAAATATTTGACTCTCACTAATAAGTAGTGCTAAATATCTGACCACTAGAAAATATAAAACACAAAAATAAATTTTCTTTAATAAAATAAGATGAATTATCAAATATTTGAAGGTGGTAAGTAATATTTTACTTTGGTGAAAGAGAATAATATGTCGTTTTCCAAAAATATGTCTGGACCAATGATCTCTGGCCCGTGAAAATTGAAAAACGCTTCGCTCTGTCGTCTGGTTAATTTCCAAGATTCCCGAGTGATAGTTTTGGTAATTAATTCAGAAAATAAAAGAAATAAAATATTGTTTGCTGTTTTTTCTTTTTTGGCAGCGGCGGTGGGGGAAGGATTTCTGGCGCGTGACATCACGTTCCAGAACACGGCGGGCCCATCGAAGCACCAAGCGGTGGCTCTCCGCGTCGGAGCGGATCTCTCCGCCTTCTACCAATGCGATATGTTGGCTTACCAAGACACTCTCTACGTCCATTCCAACCGTCAGTTCTACATTAACTGCCTTGTCTCCGGCACCGTCGATTTCATCTTCGGCAACGCGGCGGCGGTTTTCCAGGACTGTGACATCCACGCTCGTCGGCCCAACTCCGGCCAGAAGAACATGGTCACGGCTCAGGGACGGTCCGACCCGAACCAGAACACCGGGATCGTGATTCAGAAATCCCGAATCGGAGCCACCTCAGATCTCCGGTCGGTTCAGAAAAACTTCCCGACGTTTCTCGGAAGGCCATGGAAGGAATATTCTAGAACCGTGATTATGCAGTCGTCGATAAGTGACGTCATCGACCCCAAGGGGTGGCACGAGTGGAGCGGTAATTTCGCGCTCAATACGTTGTTCTACGCCGAGTATCAGAACACCGGCCCGGGGGCTTCGACGGCCGGGAGAGTGACGTGGAAAGGATTTAAAGTAATTCGAAGTGCCACGGAGGCCGAACCTTTCACCGCCGGCAGATTCATCGGCGGCGGGAGTTGGCTGCGGGCAACTGGTTTTCCGTTCTCCCTAGGCCTATAA

mRNA sequence

ATGGAGAAACCCATCGTCGCCGGAATGGAAATCTCAGATTCCGGTCACCGGAGCACTCCCGTCCCCCGAAACAGAAACAGCAAGAAGACGCTGCTTCTGGCTCTGTCCGCAGCTCTCCTCGTGGTCGGCGCCGTCGTGGGCATTGTCGCCGGCGTGACGAAGTCGAGAAACTCCGGCCGCCAGTCGGAAGAAGCCCACGCCATTGTGAAGAGCACGTGCAGCGCCACGCTGTACCCGGAGCTGTGCTTCTCGACGATTTCCCGGAGCGAGGGAGCGATGAAGAAAGTTTCGAATCAGAAGGACGTGATTGAGCTGTCGATCAACATAACGGTGAAGGCGGTGGAGGAGAATTACTTCAGAGTGCAGAAATTGGCGGCGCTGAAGAACATAACCCACCGCGAGAGAATTGCGCTCCACGATTGCCTTGAGACCATCGACGAGACGCTGGATGAGCTCCACAAGTCCATCGTGGACCTCCATCAGTACCCCAATAAGAAGTCTCTCGACCAGCACGCCGACGACCTCAAGACCCTGCTCAGTTCCGCCATTACCAATCAGGAAACTTGCTTGGATGGATTCTCACACGACGACGCGGATAAAAGCCTGCGGAAGAAGCTGGAAGAGGGGCAGAAGGAAGTGGAGCACATGTGCAGCAATGCACTCGCCATGATCAAGAACATGACCGATACCGATATTGCCAACTACCACGCCAAAATGGGAGCTAACAGAAACCTCATTGAGAACGCCGACGCCGGAGACGCCGCCATCATCTGGCCCCACTGGCTCTCGCCCGGAGACCGCCGCCTTCTGCAGTCCTCCTCCGTCACGCCGGACGCGGTCGTGGCGGCGGACGGCAGTGGGAACTTCCGGACGGTGTCTGCAGCCGTGTCCGCCGCGCCGGACAAAAGCAAGAAGAGGTACATTATTAGAATCAAAGCCGGCGTCTACAGGGAGAATGTGGAAATTCCCAAGAAGAAAACCAATATAATGTTTGTGGGAGATGGACGAAGCAACACCATTATCACCGGCAGCCGGAATGTCGTCGACGGCAGCACCACTTTCAACTCTGCAACCGTCGCGGCGGTGGGGGAAGGATTTCTGGCGCGTGACATCACGTTCCAGAACACGGCGGGCCCATCGAAGCACCAAGCGGTGGCTCTCCGCGTCGGAGCGGATCTCTCCGCCTTCTACCAATGCGATATGTTGGCTTACCAAGACACTCTCTACGTCCATTCCAACCGTCAGTTCTACATTAACTGCCTTGTCTCCGGCACCGTCGATTTCATCTTCGGCAACGCGGCGGCGGTTTTCCAGGACTGTGACATCCACGCTCGTCGGCCCAACTCCGGCCAGAAGAACATGGTCACGGCTCAGGGACGGTCCGACCCGAACCAGAACACCGGGATCGTGATTCAGAAATCCCGAATCGGAGCCACCTCAGATCTCCGGTCGGTTCAGAAAAACTTCCCGACGTTTCTCGGAAGGCCATGGAAGGAATATTCTAGAACCGTGATTATGCAGTCGTCGATAAGTGACGTCATCGACCCCAAGGGGTGGCACGAGTGGAGCGGTAATTTCGCGCTCAATACGTTGTTCTACGCCGAGTATCAGAACACCGGCCCGGGGGCTTCGACGGCCGGGAGAGTGACGTGGAAAGGATTTAAAGTAATTCGAAGTGCCACGGAGGCCGAACCTTTCACCGCCGGCAGATTCATCGGCGGCGGGAGTTGGCTGCGGGCAACTGGTTTTCCGTTCTCCCTAGGCCTATAA

Coding sequence (CDS)

ATGGAGAAACCCATCGTCGCCGGAATGGAAATCTCAGATTCCGGTCACCGGAGCACTCCCGTCCCCCGAAACAGAAACAGCAAGAAGACGCTGCTTCTGGCTCTGTCCGCAGCTCTCCTCGTGGTCGGCGCCGTCGTGGGCATTGTCGCCGGCGTGACGAAGTCGAGAAACTCCGGCCGCCAGTCGGAAGAAGCCCACGCCATTGTGAAGAGCACGTGCAGCGCCACGCTGTACCCGGAGCTGTGCTTCTCGACGATTTCCCGGAGCGAGGGAGCGATGAAGAAAGTTTCGAATCAGAAGGACGTGATTGAGCTGTCGATCAACATAACGGTGAAGGCGGTGGAGGAGAATTACTTCAGAGTGCAGAAATTGGCGGCGCTGAAGAACATAACCCACCGCGAGAGAATTGCGCTCCACGATTGCCTTGAGACCATCGACGAGACGCTGGATGAGCTCCACAAGTCCATCGTGGACCTCCATCAGTACCCCAATAAGAAGTCTCTCGACCAGCACGCCGACGACCTCAAGACCCTGCTCAGTTCCGCCATTACCAATCAGGAAACTTGCTTGGATGGATTCTCACACGACGACGCGGATAAAAGCCTGCGGAAGAAGCTGGAAGAGGGGCAGAAGGAAGTGGAGCACATGTGCAGCAATGCACTCGCCATGATCAAGAACATGACCGATACCGATATTGCCAACTACCACGCCAAAATGGGAGCTAACAGAAACCTCATTGAGAACGCCGACGCCGGAGACGCCGCCATCATCTGGCCCCACTGGCTCTCGCCCGGAGACCGCCGCCTTCTGCAGTCCTCCTCCGTCACGCCGGACGCGGTCGTGGCGGCGGACGGCAGTGGGAACTTCCGGACGGTGTCTGCAGCCGTGTCCGCCGCGCCGGACAAAAGCAAGAAGAGGTACATTATTAGAATCAAAGCCGGCGTCTACAGGGAGAATGTGGAAATTCCCAAGAAGAAAACCAATATAATGTTTGTGGGAGATGGACGAAGCAACACCATTATCACCGGCAGCCGGAATGTCGTCGACGGCAGCACCACTTTCAACTCTGCAACCGTCGCGGCGGTGGGGGAAGGATTTCTGGCGCGTGACATCACGTTCCAGAACACGGCGGGCCCATCGAAGCACCAAGCGGTGGCTCTCCGCGTCGGAGCGGATCTCTCCGCCTTCTACCAATGCGATATGTTGGCTTACCAAGACACTCTCTACGTCCATTCCAACCGTCAGTTCTACATTAACTGCCTTGTCTCCGGCACCGTCGATTTCATCTTCGGCAACGCGGCGGCGGTTTTCCAGGACTGTGACATCCACGCTCGTCGGCCCAACTCCGGCCAGAAGAACATGGTCACGGCTCAGGGACGGTCCGACCCGAACCAGAACACCGGGATCGTGATTCAGAAATCCCGAATCGGAGCCACCTCAGATCTCCGGTCGGTTCAGAAAAACTTCCCGACGTTTCTCGGAAGGCCATGGAAGGAATATTCTAGAACCGTGATTATGCAGTCGTCGATAAGTGACGTCATCGACCCCAAGGGGTGGCACGAGTGGAGCGGTAATTTCGCGCTCAATACGTTGTTCTACGCCGAGTATCAGAACACCGGCCCGGGGGCTTCGACGGCCGGGAGAGTGACGTGGAAAGGATTTAAAGTAATTCGAAGTGCCACGGAGGCCGAACCTTTCACCGCCGGCAGATTCATCGGCGGCGGGAGTTGGCTGCGGGCAACTGGTTTTCCGTTCTCCCTAGGCCTATAA

Protein sequence

MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGRQSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMGANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL
Homology
BLAST of Moc09g01520 vs. NCBI nr
Match: XP_022153038.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR
Sbjct: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
           QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR
Sbjct: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS
Sbjct: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG
Sbjct: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240

Query: 241 ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP 300
           ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP
Sbjct: 241 ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP 300

Query: 301 DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360
           DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA
Sbjct: 301 DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420

Query: 421 LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 421 LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480

Query: 481 DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG 540
           DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG
Sbjct: 481 DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG 540

Query: 541 PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL
Sbjct: 541 PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 589

BLAST of Moc09g01520 vs. NCBI nr
Match: XP_004141734.1 (pectinesterase [Cucumis sativus] >KGN45437.1 hypothetical protein Csa_016439 [Cucumis sativus])

HSP 1 Score: 969.9 bits (2506), Expect = 9.7e-279
Identity = 492/593 (82.97%), Postives = 536/593 (90.39%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           ME+PI AG  +S+S        +  + KK L++ L + LL+VGAV+GIVAG TKS  S +
Sbjct: 7   MEQPIAAGAGVSNSS--DLQKTQKSSKKKLLIVTLCSTLLIVGAVIGIVAGTTKS--SSK 66

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
            S E+HAIVKSTCS+TLYP++C+S ISRSEGAM KV NQKDVIE+SINITVKAVEENYFR
Sbjct: 67  YSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFR 126

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           V+KL  LKNI+HRERIALHDCLETIDETLDELHK+IVDL++YPNKKSL+QHADDLKTLLS
Sbjct: 127 VKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLS 186

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHDDADK+LR KL+EGQ EVEHMCSNALAMIKNMTDTDIANY AKMG
Sbjct: 187 SAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMG 246

Query: 241 --ANRNLI--ENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAV 300
              NR L+  E+ +  D  I WP WLS GDRRLLQSSSVTP+ VVAADGSGNFRTV+AAV
Sbjct: 247 ITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAV 306

Query: 301 SAAPDKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNS 360
           +AAP +S KRY+IRIKAGVYRENVE+PKKKTNIMF+GDGR NTIITGSRNVVDGSTTFNS
Sbjct: 307 AAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNS 366

Query: 361 ATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF 420
           AT+AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF
Sbjct: 367 ATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF 426

Query: 421 YINCLVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRI 480
           YINCLVSGTVDFIFGNAAA+FQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRI
Sbjct: 427 YINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRI 486

Query: 481 GATSDLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEY 540
           GATSDLR VQK+FPTFLGRPWKEYSRTVIMQ +ISDVIDPKGWHEWSG+FALNTLFY EY
Sbjct: 487 GATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEY 546

Query: 541 QNTGPGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           QNTG GAST GRVTWKGF+VIRSATEAE FTAG+FIGGGSWL +TGFPFSLGL
Sbjct: 547 QNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595

BLAST of Moc09g01520 vs. NCBI nr
Match: KAG7013952.1 (hypothetical protein SDJN02_24121 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 968.0 bits (2501), Expect = 3.7e-278
Identity = 492/589 (83.53%), Postives = 533/589 (90.49%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           ME+PI AG  IS SG+   P P+N N KK ++LA+S+ LL    +VGIVAGV  SRN G 
Sbjct: 1   MEQPIAAG--ISSSGNH-LPKPKN-NRKKLIILAVSSTLL----LVGIVAGVVSSRNGGG 60

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
           +  + HAIVKSTCS+TLYP+LCFS+I +S+GAMK+V ++KDVIE+SINITVKAVEENYF+
Sbjct: 61  EEAKRHAIVKSTCSSTLYPDLCFSSILKSDGAMKRVGSKKDVIEVSINITVKAVEENYFK 120

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           V KL  LKNI+HRE+IALHDCLETIDETLDELHK+ VDLH+YPNKKSL+QHADDLKTLLS
Sbjct: 121 VLKLYELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLNQHADDLKTLLS 180

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHD+ADK+LR KL+EGQ EVE MCSNALAMIKNMTDTDIANY AKMG
Sbjct: 181 SAITNQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYEAKMG 240

Query: 241 ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP 300
              N     +  D  I WP WLS GDRRLLQSSSV PD VVAADGSGNF+TV+AAV+AAP
Sbjct: 241 TTANRKLKEEINDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAP 300

Query: 301 DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360
            KS KRY+IRIKAGVYRENV++PKKKTNIMF+GDGRSNTIITGSRNVVDGSTTFNSATVA
Sbjct: 301 VKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVA 360

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420

Query: 421 LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           L+SGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 421 LISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480

Query: 481 DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG 540
           DLRSVQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFY EYQNTG
Sbjct: 481 DLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTG 540

Query: 541 PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           PGAST+GRVTWKGF+VIRSATEAE FT G+FIGGGSWLRATGFPFSLGL
Sbjct: 541 PGASTSGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of Moc09g01520 vs. NCBI nr
Match: KAA0059355.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK03971.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 967.6 bits (2500), Expect = 4.8e-278
Identity = 493/593 (83.14%), Postives = 537/593 (90.56%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           ME+PI AG  IS+S   S      + SKK L+L LS+ LL+VGAV+GIVAG TK   S +
Sbjct: 7   MEQPIAAGAGISNS---SDLKKNKKASKKLLILTLSSTLLIVGAVIGIVAGTTK---SSK 66

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
            S ++HAIVKSTCS+TLYP++C+S IS+SEGAM KV +QKDVIE+SINITVKAVEENYFR
Sbjct: 67  DSGKSHAIVKSTCSSTLYPDMCYSAISKSEGAMLKVKSQKDVIEVSINITVKAVEENYFR 126

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           V+KL  LKNI+HRE+IALHDCLETIDETLDELHK+IVDL++YPNKKSL+QHADDLKTLLS
Sbjct: 127 VKKLCGLKNISHREKIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLS 186

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHD+ADK+LR KL+ GQ EVEHMCSNALAMIKNMTDTDIANY AKMG
Sbjct: 187 SAITNQETCLDGFSHDNADKTLRDKLKAGQMEVEHMCSNALAMIKNMTDTDIANYEAKMG 246

Query: 241 --ANRNLI--ENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAV 300
              NR L+  E  +  +  I WP WLS GDRRLLQSSSVTP+ VVAADGSGNFRTV+AAV
Sbjct: 247 ITKNRKLMAEEEDNNNNDEIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAV 306

Query: 301 SAAPDKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNS 360
           +AAP+KS KRY+IRIKAGVYRENVE+PKKKTNIMF+GDGR NTIITGSRNVVDGSTTF S
Sbjct: 307 AAAPEKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKS 366

Query: 361 ATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF 420
           ATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF
Sbjct: 367 ATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF 426

Query: 421 YINCLVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRI 480
           YINCLVSGTVDFIFGNAAA+FQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRI
Sbjct: 427 YINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRI 486

Query: 481 GATSDLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEY 540
           GATSDLR VQK+FPTFLGRPWKEYSRTVIMQ SISDVIDPKGWHEWSG+FALNTLFY EY
Sbjct: 487 GATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCSISDVIDPKGWHEWSGSFALNTLFYGEY 546

Query: 541 QNTGPGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           QNTGPGAST GRVTWKGF+VIRSATEAE FTAG+FIGGGSWLR+TGFPFSLGL
Sbjct: 547 QNTGPGASTGGRVTWKGFRVIRSATEAEGFTAGKFIGGGSWLRSTGFPFSLGL 593

BLAST of Moc09g01520 vs. NCBI nr
Match: XP_022953820.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 967.2 bits (2499), Expect = 6.3e-278
Identity = 491/589 (83.36%), Postives = 534/589 (90.66%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           ME+PI AG  IS SG+   P P+N N KK ++LA+S+ LL    +VGIVAGV  SRN G 
Sbjct: 1   MEQPIAAG--ISSSGNH-LPKPKN-NRKKLIILAVSSTLL----LVGIVAGVVSSRNGGG 60

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
           +  ++HAIVKSTCS+TLYP+LCFS+I +S+GAMK+V ++KDVIE+SINITVKAVEENYF+
Sbjct: 61  EEAKSHAIVKSTCSSTLYPDLCFSSILKSDGAMKRVGSKKDVIEVSINITVKAVEENYFK 120

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           V KL  LKNI+HRE+IALHDCLETIDETLDELHK+ VDLH+YPNKKSL+QHADDLKTLLS
Sbjct: 121 VLKLYELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLNQHADDLKTLLS 180

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHD+ADK+LR KL+EGQ EVE MCSNALAMIKNMTDTDIANY AKMG
Sbjct: 181 SAITNQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYEAKMG 240

Query: 241 ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP 300
              N     +  D  I WP WLS GDRRLLQSSSV PD VVAADGSGNF+TV+AAV+AAP
Sbjct: 241 TTANRKLKEEINDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAP 300

Query: 301 DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360
            KS KR++IRIKAGVYRENV++PKKKTNIMF+GDGRSNTIITGSRNVVDGSTTFNSATVA
Sbjct: 301 VKSSKRFVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVA 360

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420

Query: 421 LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           L+SGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 421 LISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480

Query: 481 DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG 540
           DLRSVQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFY EYQNTG
Sbjct: 481 DLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTG 540

Query: 541 PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           PGAST+GRVTWKGF+VIRSATEAE FT G+FIGGGSWLRATGFPFSLGL
Sbjct: 541 PGASTSGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of Moc09g01520 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 2.2e-241
Identity = 425/579 (73.40%), Postives = 479/579 (82.73%), Query Frame = 0

Query: 12  SDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGRQSEEA-HAIVK 71
           S S    + +P+    KK L LAL A LLVV AV+GIVAGV   +NSG    E  HAI+K
Sbjct: 14  SSSNQNISNIPK---KKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILK 73

Query: 72  STCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKLAALKNI 131
           S+CS+T YP+LCFS I+    A KKV++QKDVIE+S+NIT  AVE NYF +QKL    N+
Sbjct: 74  SSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNL 133

Query: 132 THRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQETCL 191
           T RE++ALHDCLETIDETLDELHK++ DL +YPNKKSL QHADDLKTL+S+A+TNQ TCL
Sbjct: 134 TKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCL 193

Query: 192 DGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMGANRNLIENAD 251
           DGFSHDDA+K +R  L +GQ  VE MCSNALAMIKNMTDTD+     +   NR L E   
Sbjct: 194 DGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDM--MIMRTSNNRKLTEETS 253

Query: 252 AGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKRYIIR 311
             D    WP WLSPGDRRLLQSSSVTP+AVVAADGSGNF+TV+AAV+AAP    KRYIIR
Sbjct: 254 TVDG---WPAWLSPGDRRLLQSSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIR 313

Query: 312 IKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARD 371
           IKAGVYRENVE+ KK  NIMF+GDGR+ TIITGSRNVVDGSTTF SAT A VGEGFLARD
Sbjct: 314 IKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARD 373

Query: 372 ITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIF 431
           ITFQNTAGPSKHQAVALRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCL++GTVDFIF
Sbjct: 374 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 433

Query: 432 GNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKNFP 491
           GNAAAV Q+CDIHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+ VQ +FP
Sbjct: 434 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFP 493

Query: 492 TFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGRVT 551
           T+LGRPWKEYSRTVIMQSSI+D+I P GWHEW GNFALNTLFY E+QN+G GA T+GRV 
Sbjct: 494 TYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVK 553

Query: 552 WKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           WKGF+VI SATEA+ FT G FI G SWL +TGFPFSLGL
Sbjct: 554 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Moc09g01520 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 1.4e-240
Identity = 422/563 (74.96%), Postives = 472/563 (83.84%), Query Frame = 0

Query: 28  KKTLLLALSAALLVVGAVVGIVAGVTKSRNSGRQSEEA-HAIVKSTCSATLYPELCFSTI 87
           KK L LAL A LLVV AV+GIVAGV   +NSG    E  HAI+KS+CS+T YP+LCFS I
Sbjct: 27  KKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAI 86

Query: 88  SRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKLAALKNITHRERIALHDCLETID 147
           +    A KKV++QKDVIE+S+NIT  AVE NYF +QKL    N+T RE++ALHDCLETID
Sbjct: 87  AAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETID 146

Query: 148 ETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQETCLDGFSHDDADKSLRKKL 207
           ETLDELHK++ DL +YPNKKSL QHADDLKTL+S+A+TNQ TCLDGFSHDDA+K +R  L
Sbjct: 147 ETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDAL 206

Query: 208 EEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMGANRNLIENADAGDAAIIWPHWLSPGD 267
            +GQ  VE MCSNALAMIKNMTDTD+     +   NR LIE     D    WP WLS GD
Sbjct: 207 SDGQVHVEKMCSNALAMIKNMTDTDM--MIMRTSNNRKLIEETSTVDG---WPAWLSTGD 266

Query: 268 RRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKRYIIRIKAGVYRENVEIPKKK 327
           RRLLQSSSVTP+ VVAADGSGNF+TV+A+V+AAP    KRYIIRIKAGVYRENVE+ KK 
Sbjct: 267 RRLLQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVYRENVEVTKKH 326

Query: 328 TNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNTAGPSKHQAVA 387
            NIMF+GDGR+ TIITGSRNVVDGSTTF SATVA VGEGFLARDITFQNTAGPSKHQAVA
Sbjct: 327 KNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNTAGPSKHQAVA 386

Query: 388 LRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAVFQDCDIHARR 447
           LRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCL++GTVDFIFGNAAAV Q+CDIHAR+
Sbjct: 387 LRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARK 446

Query: 448 PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKNFPTFLGRPWKEYSRTVIM 507
           PNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+ VQ +FPT+LGRPWKEYSRTVIM
Sbjct: 447 PNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIM 506

Query: 508 QSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGRVTWKGFKVIRSATEAEPF 567
           QSSI+DVI P GWHEW GNFALNTLFY E+QN G GA T+GRV WKGF+VI SATEA+ F
Sbjct: 507 QSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAF 566

Query: 568 TAGRFIGGGSWLRATGFPFSLGL 590
           T G FI G SWL +TGFPFSLGL
Sbjct: 567 TPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Moc09g01520 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 1.6e-231
Identity = 416/573 (72.60%), Postives = 475/573 (82.90%), Query Frame = 0

Query: 28  KKTLLLALSAALLVVGAVVGIVAGV-TKSRNSGRQ------SEEAHAIVKSTCSATLYPE 87
           KK + LA+ A++L+V AV+G+VAGV + S+NS         S  AHAIVKS CS TL+PE
Sbjct: 18  KKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHPE 77

Query: 88  LCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKL-AALKNITHRERIALH 147
           LC+S I       KKV++QKDVIELS+NITVKAV  NY+ V++L    K +T RE++ALH
Sbjct: 78  LCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALH 137

Query: 148 DCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQETCLDGFSHDDAD 207
           DCLET+DETLDELH ++ DL  YPNKKSL +H +DLKTL+SSAITNQETCLDGFSHD+AD
Sbjct: 138 DCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDEAD 197

Query: 208 KSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMGA---NRNLIENADAGDAAI 267
           K +RK L +GQK VE MCSNALAMI NMTDTDIAN   K+ A   NR L+E  D G+   
Sbjct: 198 KKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIAN-EMKLSAPANNRKLVE--DNGE--- 257

Query: 268 IWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKRYIIRIKAGVY 327
            WP WLS GDRRLLQSS+VTPD VVAADGSG+++TVS AV  AP+KS KRY+IRIKAGVY
Sbjct: 258 -WPEWLSAGDRRLLQSSTVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVY 317

Query: 328 RENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNT 387
           RENV++PKKKTNIMF+GDG+SNTIIT SRNV DGSTTF+SATV  V    LARDITFQNT
Sbjct: 318 RENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNT 377

Query: 388 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAV 447
           AG SKHQAVAL VG+DLSAFY+CDMLAYQDTLYVHSNRQF++ CLV+GTVDFIFGN AAV
Sbjct: 378 AGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAV 437

Query: 448 FQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKNFPTFLGRP 507
           FQDCDIHARRP SGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDLR VQK+FPT+LGRP
Sbjct: 438 FQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRP 497

Query: 508 WKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGRVTWKGFKV 567
           WKEYSRTVIMQSSI+DVI P GWHEW+GNFAL+TLFY EY NTG GA T+GRV WKG KV
Sbjct: 498 WKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKV 557

Query: 568 IRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           I S+TEA+ +T GRFI GGSWL +TGFPFSLGL
Sbjct: 558 ITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of Moc09g01520 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 792.3 bits (2045), Expect = 3.6e-228
Identity = 404/580 (69.66%), Postives = 471/580 (81.21%), Query Frame = 0

Query: 25  RNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGRQSEEAHAIVKSTCSATLYPELCFS 84
           + +KK +LL+ + ALL V AV GI AG +K+      S  +HA+++S+CS+T YPELC S
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCIS 74

Query: 85  TISRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKL-AALKNITHRERIALHDCLE 144
            +  + G   ++++QKDVIE S+N+T+ AVE NYF V+KL    K +T RE+ ALHDCLE
Sbjct: 75  AVVTAGGV--ELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLE 134

Query: 145 TIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQETCLDGFSHDDADKSLR 204
           TIDETLDELH+++ DLH YP KK+L +HA DLKTL+SSAITNQETCLDGFSHDDADK +R
Sbjct: 135 TIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVR 194

Query: 205 KKLEEGQKEVEHMCSNALAMIKNMTDTDIANYH--AKMGANRNLI--ENADAGDAAII-- 264
           K L +GQ  VEHMCSNALAMIKNMTDTDIAN+   AK+ +N   +  EN +   A  I  
Sbjct: 195 KALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAG 254

Query: 265 --------WPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKRYII 324
                   WP WLS GDRRLLQ S V  DA VAADGSG F+TV+AAV+AAP+ S KRY+I
Sbjct: 255 AGELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVI 314

Query: 325 RIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLAR 384
            IKAGVYRENVE+ KKK NIMF+GDGR+ TIITGSRNVVDGSTTF+SATVAAVGE FLAR
Sbjct: 315 HIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLAR 374

Query: 385 DITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFI 444
           DITFQNTAGPSKHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF++ CL++GTVDFI
Sbjct: 375 DITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFI 434

Query: 445 FGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKNF 504
           FGNAA V QDCDIHARRPNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+SV+ +F
Sbjct: 435 FGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSF 494

Query: 505 PTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGRV 564
           PT+LGRPWKEYS+TVIMQS+ISDVI P+GW EW+G FALNTL Y EY NTG GA TA RV
Sbjct: 495 PTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRV 554

Query: 565 TWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
            W+GFKVI +A EA+ +TAG+FIGGG WL +TGFPFSLGL
Sbjct: 555 KWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Moc09g01520 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 780.0 bits (2013), Expect = 1.9e-224
Identity = 402/576 (69.79%), Postives = 469/576 (81.42%), Query Frame = 0

Query: 25  RNSKKTLLLALSAALLVVGAVVGIVAGVT---KSRNSGRQSEEAHAIVKSTCSATLYPEL 84
           +N+KK +L + + ALL++ ++VGI A  T   K++     S  +HAI+KS CS+TLYPEL
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 85  CFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKL-AALKNITHRERIALHD 144
           CFS ++ + G  K++++QK+VIE S+N+T KAV+ NYF V+KL A  K +T RE  ALHD
Sbjct: 75  CFSAVAATGG--KELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHD 134

Query: 145 CLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQETCLDGFSHDDADK 204
           CLETIDETLDELH ++ DLHQYP +KSL +HADDLKTL+SSAITNQ TCLDGFS+DDAD+
Sbjct: 135 CLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADR 194

Query: 205 SLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAK-------MGANRNLIENADAGD 264
            +RK L +GQ  VEHMCSNALAMIKNMT+TDIAN+  +          NR L E     D
Sbjct: 195 KVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLD 254

Query: 265 AAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKRYIIRIKA 324
           +   WP WLS GDRRLLQ S++  DA VA DGSG+F TV+AAV+AAP+KS KR++I IKA
Sbjct: 255 SD-GWPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKA 314

Query: 325 GVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITF 384
           GVYRENVE+ KKKTNIMF+GDGR  TIITGSRNVVDGSTTF+SATVAAVGE FLARDITF
Sbjct: 315 GVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITF 374

Query: 385 QNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNA 444
           QNTAGPSKHQAVALRVG+D SAFYQCDM AYQDTLYVHSNRQF++ C ++GTVDFIFGNA
Sbjct: 375 QNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNA 434

Query: 445 AAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKNFPTFL 504
           AAV QDCDI+ARRPNSGQKNMVTAQGRSDPNQNTGIVIQ  RIG TSDL +V+  FPT+L
Sbjct: 435 AAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYL 494

Query: 505 GRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGRVTWKG 564
           GRPWKEYSRTVIMQS ISDVI P+GWHEWSG+FAL+TL Y EY N G GA TA RV WKG
Sbjct: 495 GRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKG 554

Query: 565 FKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           +KVI S TEA+PFTAG+FIGGG WL +TGFPFSL L
Sbjct: 555 YKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Moc09g01520 vs. ExPASy TrEMBL
Match: A0A6J1DGG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020634 PE=3 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR
Sbjct: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
           QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR
Sbjct: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS
Sbjct: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG
Sbjct: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240

Query: 241 ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP 300
           ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP
Sbjct: 241 ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP 300

Query: 301 DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360
           DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA
Sbjct: 301 DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420

Query: 421 LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 421 LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480

Query: 481 DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG 540
           DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG
Sbjct: 481 DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG 540

Query: 541 PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL
Sbjct: 541 PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 589

BLAST of Moc09g01520 vs. ExPASy TrEMBL
Match: A0A0A0KAM6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G447990 PE=3 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 4.7e-279
Identity = 492/593 (82.97%), Postives = 536/593 (90.39%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           ME+PI AG  +S+S        +  + KK L++ L + LL+VGAV+GIVAG TKS  S +
Sbjct: 7   MEQPIAAGAGVSNSS--DLQKTQKSSKKKLLIVTLCSTLLIVGAVIGIVAGTTKS--SSK 66

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
            S E+HAIVKSTCS+TLYP++C+S ISRSEGAM KV NQKDVIE+SINITVKAVEENYFR
Sbjct: 67  YSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFR 126

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           V+KL  LKNI+HRERIALHDCLETIDETLDELHK+IVDL++YPNKKSL+QHADDLKTLLS
Sbjct: 127 VKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLS 186

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHDDADK+LR KL+EGQ EVEHMCSNALAMIKNMTDTDIANY AKMG
Sbjct: 187 SAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMG 246

Query: 241 --ANRNLI--ENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAV 300
              NR L+  E+ +  D  I WP WLS GDRRLLQSSSVTP+ VVAADGSGNFRTV+AAV
Sbjct: 247 ITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAV 306

Query: 301 SAAPDKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNS 360
           +AAP +S KRY+IRIKAGVYRENVE+PKKKTNIMF+GDGR NTIITGSRNVVDGSTTFNS
Sbjct: 307 AAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNS 366

Query: 361 ATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF 420
           AT+AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF
Sbjct: 367 ATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF 426

Query: 421 YINCLVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRI 480
           YINCLVSGTVDFIFGNAAA+FQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRI
Sbjct: 427 YINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRI 486

Query: 481 GATSDLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEY 540
           GATSDLR VQK+FPTFLGRPWKEYSRTVIMQ +ISDVIDPKGWHEWSG+FALNTLFY EY
Sbjct: 487 GATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEY 546

Query: 541 QNTGPGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           QNTG GAST GRVTWKGF+VIRSATEAE FTAG+FIGGGSWL +TGFPFSLGL
Sbjct: 547 QNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595

BLAST of Moc09g01520 vs. ExPASy TrEMBL
Match: A0A5A7V0J0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001680 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.3e-278
Identity = 493/593 (83.14%), Postives = 537/593 (90.56%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           ME+PI AG  IS+S   S      + SKK L+L LS+ LL+VGAV+GIVAG TK   S +
Sbjct: 7   MEQPIAAGAGISNS---SDLKKNKKASKKLLILTLSSTLLIVGAVIGIVAGTTK---SSK 66

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
            S ++HAIVKSTCS+TLYP++C+S IS+SEGAM KV +QKDVIE+SINITVKAVEENYFR
Sbjct: 67  DSGKSHAIVKSTCSSTLYPDMCYSAISKSEGAMLKVKSQKDVIEVSINITVKAVEENYFR 126

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           V+KL  LKNI+HRE+IALHDCLETIDETLDELHK+IVDL++YPNKKSL+QHADDLKTLLS
Sbjct: 127 VKKLCGLKNISHREKIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLS 186

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHD+ADK+LR KL+ GQ EVEHMCSNALAMIKNMTDTDIANY AKMG
Sbjct: 187 SAITNQETCLDGFSHDNADKTLRDKLKAGQMEVEHMCSNALAMIKNMTDTDIANYEAKMG 246

Query: 241 --ANRNLI--ENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAV 300
              NR L+  E  +  +  I WP WLS GDRRLLQSSSVTP+ VVAADGSGNFRTV+AAV
Sbjct: 247 ITKNRKLMAEEEDNNNNDEIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAV 306

Query: 301 SAAPDKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNS 360
           +AAP+KS KRY+IRIKAGVYRENVE+PKKKTNIMF+GDGR NTIITGSRNVVDGSTTF S
Sbjct: 307 AAAPEKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKS 366

Query: 361 ATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF 420
           ATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF
Sbjct: 367 ATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQF 426

Query: 421 YINCLVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRI 480
           YINCLVSGTVDFIFGNAAA+FQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRI
Sbjct: 427 YINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRI 486

Query: 481 GATSDLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEY 540
           GATSDLR VQK+FPTFLGRPWKEYSRTVIMQ SISDVIDPKGWHEWSG+FALNTLFY EY
Sbjct: 487 GATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCSISDVIDPKGWHEWSGSFALNTLFYGEY 546

Query: 541 QNTGPGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           QNTGPGAST GRVTWKGF+VIRSATEAE FTAG+FIGGGSWLR+TGFPFSLGL
Sbjct: 547 QNTGPGASTGGRVTWKGFRVIRSATEAEGFTAGKFIGGGSWLRSTGFPFSLGL 593

BLAST of Moc09g01520 vs. ExPASy TrEMBL
Match: A0A6J1GQR0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456237 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 3.0e-278
Identity = 491/589 (83.36%), Postives = 534/589 (90.66%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           ME+PI AG  IS SG+   P P+N N KK ++LA+S+ LL    +VGIVAGV  SRN G 
Sbjct: 1   MEQPIAAG--ISSSGNH-LPKPKN-NRKKLIILAVSSTLL----LVGIVAGVVSSRNGGG 60

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
           +  ++HAIVKSTCS+TLYP+LCFS+I +S+GAMK+V ++KDVIE+SINITVKAVEENYF+
Sbjct: 61  EEAKSHAIVKSTCSSTLYPDLCFSSILKSDGAMKRVGSKKDVIEVSINITVKAVEENYFK 120

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           V KL  LKNI+HRE+IALHDCLETIDETLDELHK+ VDLH+YPNKKSL+QHADDLKTLLS
Sbjct: 121 VLKLYELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLNQHADDLKTLLS 180

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHD+ADK+LR KL+EGQ EVE MCSNALAMIKNMTDTDIANY AKMG
Sbjct: 181 SAITNQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYEAKMG 240

Query: 241 ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP 300
              N     +  D  I WP WLS GDRRLLQSSSV PD VVAADGSGNF+TV+AAV+AAP
Sbjct: 241 TTANRKLKEEINDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAP 300

Query: 301 DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360
            KS KR++IRIKAGVYRENV++PKKKTNIMF+GDGRSNTIITGSRNVVDGSTTFNSATVA
Sbjct: 301 VKSSKRFVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVA 360

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420

Query: 421 LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           L+SGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 421 LISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480

Query: 481 DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG 540
           DLRSVQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFY EYQNTG
Sbjct: 481 DLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTG 540

Query: 541 PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           PGAST+GRVTWKGF+VIRSATEAE FT G+FIGGGSWLRATGFPFSLGL
Sbjct: 541 PGASTSGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of Moc09g01520 vs. ExPASy TrEMBL
Match: A0A6J1JSY6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111488604 PE=3 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 3.2e-275
Identity = 493/591 (83.42%), Postives = 533/591 (90.19%), Query Frame = 0

Query: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60
           ME+PI AG  IS SG+   P P+  N KK ++LA+S+ LL    +VGIVAGV  SRN+G 
Sbjct: 1   MEQPIAAG--ISSSGNH-LPKPK-YNRKKLIILAVSSTLL----LVGIVAGVVSSRNAGG 60

Query: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120
           +  ++HAIVKSTCS+TLYP+LCFS+I +++ AMKKVSN+KDVIE+SINITVKAVEENYF+
Sbjct: 61  EESKSHAIVKSTCSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFK 120

Query: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180
           V KL  LKNI+HRE+IALHDCLETIDETLDELHK+ VDLH+YPNKKSL Q ADDLKTLLS
Sbjct: 121 VLKLYELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLS 180

Query: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240
           SAITNQETCLDGFSHD+ADK+LR KL+EGQ EVE MCSNALAMIKNMTDTDIANY  KMG
Sbjct: 181 SAITNQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMG 240

Query: 241 --ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSA 300
             ANR L E  +  D  I WP WLS GDRRLLQSSSV PD VVAADGSGNF+TV+AAV+A
Sbjct: 241 TAANRKLKE--ENNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAA 300

Query: 301 APDKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSAT 360
           AP KS KRY+IRIKAGVYRENV++ KKKTNIMF+GDGRSNTIITGSRNVVDGSTTFNSAT
Sbjct: 301 APVKSSKRYVIRIKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSAT 360

Query: 361 VAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYI 420
           VAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYI
Sbjct: 361 VAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYI 420

Query: 421 NCLVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGA 480
           NCL+SGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGA
Sbjct: 421 NCLISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGA 480

Query: 481 TSDLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQN 540
           TSDLRSVQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFY EYQN
Sbjct: 481 TSDLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQN 540

Query: 541 TGPGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           TGPGAST GRVTWKGF+VIRSATEAE FT G+FIGGGSWLRATGFPFSLGL
Sbjct: 541 TGPGASTGGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of Moc09g01520 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 792.3 bits (2045), Expect = 2.6e-229
Identity = 404/580 (69.66%), Postives = 471/580 (81.21%), Query Frame = 0

Query: 25  RNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGRQSEEAHAIVKSTCSATLYPELCFS 84
           + +KK +LL+ + ALL V AV GI AG +K+      S  +HA+++S+CS+T YPELC S
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCIS 74

Query: 85  TISRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKL-AALKNITHRERIALHDCLE 144
            +  + G   ++++QKDVIE S+N+T+ AVE NYF V+KL    K +T RE+ ALHDCLE
Sbjct: 75  AVVTAGGV--ELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLE 134

Query: 145 TIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQETCLDGFSHDDADKSLR 204
           TIDETLDELH+++ DLH YP KK+L +HA DLKTL+SSAITNQETCLDGFSHDDADK +R
Sbjct: 135 TIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVR 194

Query: 205 KKLEEGQKEVEHMCSNALAMIKNMTDTDIANYH--AKMGANRNLI--ENADAGDAAII-- 264
           K L +GQ  VEHMCSNALAMIKNMTDTDIAN+   AK+ +N   +  EN +   A  I  
Sbjct: 195 KALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAG 254

Query: 265 --------WPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKRYII 324
                   WP WLS GDRRLLQ S V  DA VAADGSG F+TV+AAV+AAP+ S KRY+I
Sbjct: 255 AGELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVI 314

Query: 325 RIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLAR 384
            IKAGVYRENVE+ KKK NIMF+GDGR+ TIITGSRNVVDGSTTF+SATVAAVGE FLAR
Sbjct: 315 HIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLAR 374

Query: 385 DITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFI 444
           DITFQNTAGPSKHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF++ CL++GTVDFI
Sbjct: 375 DITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFI 434

Query: 445 FGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKNF 504
           FGNAA V QDCDIHARRPNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+SV+ +F
Sbjct: 435 FGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSF 494

Query: 505 PTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGRV 564
           PT+LGRPWKEYS+TVIMQS+ISDVI P+GW EW+G FALNTL Y EY NTG GA TA RV
Sbjct: 495 PTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRV 554

Query: 565 TWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
            W+GFKVI +A EA+ +TAG+FIGGG WL +TGFPFSLGL
Sbjct: 555 KWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Moc09g01520 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 780.0 bits (2013), Expect = 1.3e-225
Identity = 402/576 (69.79%), Postives = 469/576 (81.42%), Query Frame = 0

Query: 25  RNSKKTLLLALSAALLVVGAVVGIVAGVT---KSRNSGRQSEEAHAIVKSTCSATLYPEL 84
           +N+KK +L + + ALL++ ++VGI A  T   K++     S  +HAI+KS CS+TLYPEL
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 85  CFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKL-AALKNITHRERIALHD 144
           CFS ++ + G  K++++QK+VIE S+N+T KAV+ NYF V+KL A  K +T RE  ALHD
Sbjct: 75  CFSAVAATGG--KELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHD 134

Query: 145 CLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQETCLDGFSHDDADK 204
           CLETIDETLDELH ++ DLHQYP +KSL +HADDLKTL+SSAITNQ TCLDGFS+DDAD+
Sbjct: 135 CLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADR 194

Query: 205 SLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAK-------MGANRNLIENADAGD 264
            +RK L +GQ  VEHMCSNALAMIKNMT+TDIAN+  +          NR L E     D
Sbjct: 195 KVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLD 254

Query: 265 AAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKRYIIRIKA 324
           +   WP WLS GDRRLLQ S++  DA VA DGSG+F TV+AAV+AAP+KS KR++I IKA
Sbjct: 255 SD-GWPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKA 314

Query: 325 GVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITF 384
           GVYRENVE+ KKKTNIMF+GDGR  TIITGSRNVVDGSTTF+SATVAAVGE FLARDITF
Sbjct: 315 GVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITF 374

Query: 385 QNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNA 444
           QNTAGPSKHQAVALRVG+D SAFYQCDM AYQDTLYVHSNRQF++ C ++GTVDFIFGNA
Sbjct: 375 QNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNA 434

Query: 445 AAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKNFPTFL 504
           AAV QDCDI+ARRPNSGQKNMVTAQGRSDPNQNTGIVIQ  RIG TSDL +V+  FPT+L
Sbjct: 435 AAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYL 494

Query: 505 GRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGRVTWKG 564
           GRPWKEYSRTVIMQS ISDVI P+GWHEWSG+FAL+TL Y EY N G GA TA RV WKG
Sbjct: 495 GRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKG 554

Query: 565 FKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           +KVI S TEA+PFTAG+FIGGG WL +TGFPFSL L
Sbjct: 555 YKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Moc09g01520 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 443.7 bits (1140), Expect = 2.3e-124
Identity = 242/584 (41.44%), Postives = 357/584 (61.13%), Query Frame = 0

Query: 18  STPVPRNRNSKKTLLLALSAALLVVGAV-VGIVAG---VTKSRNSGRQSEEAHAIVKSTC 77
           +T +P  +   KT L+  + A+LVVG V  GI AG   V   +   + + +    +  TC
Sbjct: 20  ATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTC 79

Query: 78  SATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKLAALKNITHR 137
           S +LYP LC  T+    G++   +++ ++I +S N T++   +  +          +  R
Sbjct: 80  SKSLYPNLCIDTLLDFPGSL--TADENELIHISFNATLQKFSKALY-TSSTITYTQMPPR 139

Query: 138 ERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQETCLDGF 197
            R A   CLE +D+++D L +++  +       S D+   D+ T LSSA+TN +TC DGF
Sbjct: 140 VRSAYDSCLELLDDSVDALTRALSSVVVV----SGDESHSDVMTWLSSAMTNHDTCTDGF 199

Query: 198 SH-DDADKSLRKKLEEGQKEVEHMCSNALAM----IKNMTDTDIANYHAKMGANRNLIEN 257
              +     ++ ++    K++  M SN LA+    +K+++   + N       NR L+  
Sbjct: 200 DEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN-------NRKLLGT 259

Query: 258 ADAGDAAIIWPHWLSPGDRRLL--QSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKR 317
            +  +     P+WL   DR LL   +S++  D  V+ DGSG F+T++ A+  AP+ S +R
Sbjct: 260 EETEEL----PNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRR 319

Query: 318 YIIRIKAGVY-RENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEG 377
           ++I +KAG Y  EN+++ +KKTN+MF+GDG+  T+ITG +++ D  TTF++AT AA G G
Sbjct: 320 FVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAG 379

Query: 378 FLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGT 437
           F+ RD+TF+N AGP+KHQAVALRVG D +  Y+C+++ YQD LYVHSNRQF+  C + GT
Sbjct: 380 FIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGT 439

Query: 438 VDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSV 497
           VDFIFGNAA + Q C+I+AR+P + QK  +TAQ R DPNQNTGI I   ++ AT DL + 
Sbjct: 440 VDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEAS 499

Query: 498 QKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGAST 557
           + ++PT+LGRPWK YSR V M S + D IDP+GW EW+G FAL++L+Y EY N G G+  
Sbjct: 500 KGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGI 559

Query: 558 AGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
             RV W G+ VI S  EA  FT  +FI G SWL +TG  F  GL
Sbjct: 560 GQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of Moc09g01520 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 441.0 bits (1133), Expect = 1.5e-123
Identity = 254/521 (48.75%), Postives = 334/521 (64.11%), Query Frame = 0

Query: 69  VKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFRVQKLAALK 128
           VK+ CS T  P+ C   ++ +    + + ++ + +++S+ + +            L    
Sbjct: 30  VKAWCSQTPNPKPCEYFLTHNSN-NEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKC 89

Query: 129 NITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLSSAITNQET 188
             T RE+ A  DC++  D T+ ++++++      PN K       D +T LS+A+TN +T
Sbjct: 90  RDT-REKAAWEDCIKLYDLTVSKINETM-----DPNVKCSKL---DAQTWLSTALTNLDT 149

Query: 189 CLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMGANRNLIEN 248
           C  GF            LE G  ++       L ++ N     + N  A      N    
Sbjct: 150 CRAGF------------LELGVTDI------VLPLMSNNVSNLLCNTLAINKVPFNYTPP 209

Query: 249 ADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAPDKSKKRYI 308
              G     +P W+ PGDR+LLQSS+   +AVVA DGSGNF+T+  A+ AA      R++
Sbjct: 210 EKDG-----FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA--SGSGRFV 269

Query: 309 IRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLA 368
           I +K GVY EN+EI KK  N+M  GDG   TIITGS++V  G+TTFNSATVAAVG+GF+A
Sbjct: 270 IYVKQGVYSENLEIRKK--NVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIA 329

Query: 369 RDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDF 428
           R ITF+NTAG S  QAVALR G+DLS FYQC   AYQDTLYVHSNRQFY +C V GTVDF
Sbjct: 330 RGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDF 389

Query: 429 IFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKN 488
           IFGNAAAV Q+C+I ARRP S + N +TAQGRSDPNQNTGI+I  SR+ A SDLR V  +
Sbjct: 390 IFGNAAAVLQNCNIFARRPRS-KTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGS 449

Query: 489 FPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGR 548
             T+LGRPW++YSRTV M++S+  +IDP+GW EW GNFAL TLFYAE+QNTGPGAST+GR
Sbjct: 450 TKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGR 509

Query: 549 VTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           VTW GF+V+ SA+EA  FT G F+ GGSW+  +  PF+ GL
Sbjct: 510 VTWPGFRVLGSASEASKFTVGTFLAGGSWI-PSSVPFTSGL 511

BLAST of Moc09g01520 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 432.6 bits (1111), Expect = 5.2e-121
Identity = 232/456 (50.88%), Postives = 296/456 (64.91%), Query Frame = 0

Query: 137 ALHDCLETIDETLDELHKSIVDLHQYPNKK--SLDQHADDLKTLLSSAITNQETCLDGFS 196
           A+ DC++ +D   +EL   I+   Q PN K  S      DL+T +S+A++NQ+TCLDGF 
Sbjct: 80  AIVDCVDLLDSAAEEL-SWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGF- 139

Query: 197 HDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMGANRNLIENADAGDA 256
            +  +  ++K +  G  +V     N L M+ +                   +  A +G +
Sbjct: 140 -EGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKP-----KPKPIKAQTMTKAHSGFS 199

Query: 257 AIIWPHWLSPGDRRLLQSSSVT-PDAVVAADGSGNFRTVSAAVSAAPDKSKKRYIIRIKA 316
              +P W+ PGDR+LLQ+ ++T  DAVVAADG+GNF T+S AV AAPD S KRY+I +K 
Sbjct: 200 K--FPSWVKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKR 259

Query: 317 GVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITF 376
           GVY ENVEI KKK NIM VGDG   T+ITG+R+ +DG TTF SAT A  G GF+ARDITF
Sbjct: 260 GVYVENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITF 319

Query: 377 QNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNA 436
           QNTAGP KHQAVA+R   DL  FY+C M  YQDTLY HS RQF+  C+++GTVDFIFG+A
Sbjct: 320 QNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDA 379

Query: 437 AAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKNFPTFL 496
            AVFQ C I A++    QKN +TAQGR DPN+ TG  IQ S I A +DL        T+L
Sbjct: 380 TAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYL 439

Query: 497 GRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTGPGASTAGRVTWKG 556
           GRPWK YSRTV MQ+ +SD I+P GW EW+GNFAL+TL+Y EY N+GPGAS   RV W G
Sbjct: 440 GRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPG 499

Query: 557 FKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 590
           + V+ ++ EA  FT  + I G  WL +TG  F  GL
Sbjct: 500 YHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153038.10.0e+00100.00pectinesterase-like [Momordica charantia][more]
XP_004141734.19.7e-27982.97pectinesterase [Cucumis sativus] >KGN45437.1 hypothetical protein Csa_016439 [Cu... [more]
KAG7013952.13.7e-27883.53hypothetical protein SDJN02_24121 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAA0059355.14.8e-27883.14pectinesterase-like [Cucumis melo var. makuwa] >TYK03971.1 pectinesterase-like [... [more]
XP_022953820.16.3e-27883.36pectinesterase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O048862.2e-24173.40Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
P839481.4e-24074.96Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Q431431.6e-23172.60Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
O490063.6e-22869.66Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425341.9e-22469.79Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1DGG50.0e+00100.00Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020634 PE=3 SV=1[more]
A0A0A0KAM64.7e-27982.97Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G447990 PE=3 SV=1[more]
A0A5A7V0J02.3e-27883.14Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00168... [more]
A0A6J1GQR03.0e-27883.36Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456237 PE=3 SV=1[more]
A0A6J1JSY63.2e-27583.42Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111488604 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.12.6e-22969.66pectin methylesterase 3 [more]
AT1G53830.11.3e-22569.79pectin methylesterase 2 [more]
AT5G53370.12.3e-12441.44pectin methylesterase PCR fragment F [more]
AT2G45220.11.5e-12348.75Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G43270.15.2e-12150.88Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 64..223
e-value: 2.6E-38
score: 143.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 68..223
e-value: 1.2E-29
score: 103.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 39..227
e-value: 1.8E-27
score: 93.8
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 278..574
e-value: 8.8E-150
score: 497.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 65..228
e-value: 1.7E-33
score: 117.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 68..228
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 272..589
e-value: 2.3E-129
score: 433.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR31707:SF180PECTINESTERASEcoord: 26..588
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 26..588
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 71..228
e-value: 6.63859E-43
score: 149.126
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 303..322
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 422..431
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 275..588

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g01520.1Moc09g01520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity