Moc09g00890 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g00890
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	pectinesterase inhibitor-like
Location	chr9: 831710 .. 833480 (-)
RNA-Seq Expression	Moc09g00890
Synteny	Moc09g00890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAATTCCATCTCCATTCTACCTTTGGTCCTGTCACTTGTTCTGTTGAATACCGTGGTTCCGATGGGTGCACTTTCCCAAAACGACGCCGTTGCCATGATCTGCCCGAAAACCCGTAACCCAGGCTTCTGCACGTACGTGCTGAAATCCACGGGCAGCGCCACGGACCTGGTCGGTCTGGGCCGGTTCACGCTGAACCTGGCCCACGCGAGGGCCGGCGAGAGCCGCGCCCTGGCCCGGTCACTGGCGGCGAAGACGGCGGATCCCAAGCTCAGGGAGCGCTATGCCTCGTGCTCCGACAGCTACAACGACGCCGTTTCCAACATTGAGGACGCAACGAGGTACTTGGCCTCCGGTGACTATAACGGCGTCAATGTTGAGGCCTCGGCGGCGATGACAAATGCCGACGACTGTGAGGGAAACTTCACGGCGCCGCGGCCGGAGTCGGAGCTGACGAAGAACTCCAAGACTCTGGAAGATATATGTAGCATCATTTTGGTTATCTCCAATCTTCTCCTTGGCCGCGTTTGATTTATAAGTCTCGAAAATTTACAAACGTGGTTATGTAATAAATTAATAAACTCTTGTTTATTTCCCATCTACATTTCTTTTTTTCTTTTTCCCTTTGATTTCAATGGAGATTGGATTGGAATTTGTGAACTTTGATAGATGATGCTGATGAAGTAGATATTTTCATTCATTTTATGTTCTAATTTAGAACAGGTGGGGTTTGGTAATGTTTCTAATTTATATTTCTAACTTTATTTTTCTTGAAGTATAAGTATTAGCGTTTTTTTAAATTTTTCTTAAATTTTTTAATGAATTTTAGAAATATATATATATATTTATCTATTTGTCATGTTTTTCTTTTACGGGGAGAAAATGAAAGTGAAAAAAAAAATATTGCGAAAAAATAATAAAAATTTTAAATGTCTAATGAATAGTGGAGAAATATAAAATACGAGAAATAGATAAAAAAAAGTCAAACTTTAATTAAAGTGATACAAAATTAACAACAAAAACTAAATAGTACAGGTCTTGGTAATACAAATTATTTGTATAAGTAAAAGTGAGTATAAGTCAGCACTAATCAGTGTGTATGTATTTTCGAAGCTGCGAAATCTTCTCTTCTAGGTGAATTTTTCGTGAGTATATATAGTCAGACCAAAAATGATTTTTTTTTTGTTGGTTCAATTCGTTATAAAAAGAGATCGGAAAAAAAACCGATAACCGAACTGGTTATCGGTGGCGGCAGAGGCAGACCGTGAAAATGGGTTTTCGTTTTGCACTGTGAAGTTTGAGTTTACAAAATTGAGCACTGGGTAGTGGGTAGCTGGGCTTGAATGCCCAAATCTTCAAAATTGCCTATTTTTTCATCAATTCTATTTCTAAAGCTAATTAATTTAGATTCAGAGAGAGAAATTTCCTGCTCTGGCATGGAGATTTAGCTGGATCGCCATTGGAGCAAGATTAAGCTTCAAATCCACTTGTCTCCAGGAAGCCTCTCTTTGCGAAACTACTCTTGCCGGGGTGGATTTCGCCGTCCGTCGTTGCTTCCACCGCCGGCCAATCCAAGCCGGAGCTCCGTCTGCTAGACATTGATCACCATCCCGCTCCCTTTGAGCTTCCCCCTTCTGTTCTGATTTTTCGTCTGAATACACTGAGATCTCCTCCCTCTCGCACGCCGCCGCCCCCTTTCGGTATCAACGGCGGCTATCAGGTAACTGTTTTTGCGTTTCAGACGATCCTTGGCTCAGGCCGGCTATAA

mRNA sequence

ATGGCCAATTCCATCTCCATTCTACCTTTGGTCCTGTCACTTGTTCTGTTGAATACCGTGGTTCCGATGGGTGCACTTTCCCAAAACGACGCCGTTGCCATGATCTGCCCGAAAACCCGTAACCCAGGCTTCTGCACGTACGTGCTGAAATCCACGGGCAGCGCCACGGACCTGGTCGGTCTGGGCCGGTTCACGCTGAACCTGGCCCACGCGAGGGCCGGCGAGAGCCGCGCCCTGGCCCGGTCACTGGCGGCGAAGACGGCGGATCCCAAGCTCAGGGAGCGCTATGCCTCGTGCTCCGACAGCTACAACGACGCCGTTTCCAACATTGAGGACGCAACGAGGTACTTGGCCTCCGGTGACTATAACGGCGTCAATGTTGAGGCCTCGGCGGCGATGACAAATGCCGACGACTGTGAGGGAAACTTCACGGCGCCGCGGCCGGAGTCGGAGCTGACGAAGAACTCCAAGACTCTGGAAGATATATGTAGCATCATTTTGGTTATCTCCAATCTTCTCCTTGGCCGCCCTCTCTTTGCGAAACTACTCTTGCCGGGGTGGATTTCGCCGTCCGTCGTTGCTTCCACCGCCGGCCAATCCAAGCCGGAGCTCCGTCTGCTAGACATTGATCACCATCCCGCTCCCTTTGAGCTTCCCCCTTCTGTTCTGATTTTTCGTCTGAATACACTGAGATCTCCTCCCTCTCGCACGCCGCCGCCCCCTTTCGGTATCAACGGCGGCTATCAGGTAACTGTTTTTGCGTTTCAGACGATCCTTGGCTCAGGCCGGCTATAA

Coding sequence (CDS)

ATGGCCAATTCCATCTCCATTCTACCTTTGGTCCTGTCACTTGTTCTGTTGAATACCGTGGTTCCGATGGGTGCACTTTCCCAAAACGACGCCGTTGCCATGATCTGCCCGAAAACCCGTAACCCAGGCTTCTGCACGTACGTGCTGAAATCCACGGGCAGCGCCACGGACCTGGTCGGTCTGGGCCGGTTCACGCTGAACCTGGCCCACGCGAGGGCCGGCGAGAGCCGCGCCCTGGCCCGGTCACTGGCGGCGAAGACGGCGGATCCCAAGCTCAGGGAGCGCTATGCCTCGTGCTCCGACAGCTACAACGACGCCGTTTCCAACATTGAGGACGCAACGAGGTACTTGGCCTCCGGTGACTATAACGGCGTCAATGTTGAGGCCTCGGCGGCGATGACAAATGCCGACGACTGTGAGGGAAACTTCACGGCGCCGCGGCCGGAGTCGGAGCTGACGAAGAACTCCAAGACTCTGGAAGATATATGTAGCATCATTTTGGTTATCTCCAATCTTCTCCTTGGCCGCCCTCTCTTTGCGAAACTACTCTTGCCGGGGTGGATTTCGCCGTCCGTCGTTGCTTCCACCGCCGGCCAATCCAAGCCGGAGCTCCGTCTGCTAGACATTGATCACCATCCCGCTCCCTTTGAGCTTCCCCCTTCTGTTCTGATTTTTCGTCTGAATACACTGAGATCTCCTCCCTCTCGCACGCCGCCGCCCCCTTTCGGTATCAACGGCGGCTATCAGGTAACTGTTTTTGCGTTTCAGACGATCCTTGGCTCAGGCCGGCTATAA

Protein sequence

MANSISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVGLGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASGDYNGVNVEASAAMTNADDCEGNFTAPRPESELTKNSKTLEDICSIILVISNLLLGRPLFAKLLLPGWISPSVVASTAGQSKPELRLLDIDHHPAPFELPPSVLIFRLNTLRSPPSRTPPPPFGINGGYQVTVFAFQTILGSGRL

Homology

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BLAST of Moc09g00890 vs. NCBI nr
Match: XP_022143514.1 (pectinesterase inhibitor-like [Momordica charantia])

HSP 1 Score: 332.8 bits (852), Expect = 2.7e-87
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MANSISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVG 60
           MANSISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVG
Sbjct: 1   MANSISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVG 60

Query: 61  LGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASG 120
           LGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASG
Sbjct: 61  LGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASG 120

Query: 121 DYNGVNVEASAAMTNADDCEGNFTAPRPESELTKNSKTLEDICSIILVISNLLLGR 177
           DYNGVNVEASAAMTNADDCEGNFTAPRPESELTKNSKTLEDICSIILVISNLLLGR
Sbjct: 121 DYNGVNVEASAAMTNADDCEGNFTAPRPESELTKNSKTLEDICSIILVISNLLLGR 176

[Close]

BLAST of Moc09g00890 vs. NCBI nr
Match: XP_038896411.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 196.4 bits (498), Expect = 3.0e-46
Identity = 110/182 (60.44%), Postives = 136/182 (74.73%), Query Frame = 0


Query: 1   MANSI-----SILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSA 60
           MANSI     S+LP VLSL+  + VVPM A S ND V+ IC +TRNP FC+ +LKS    
Sbjct: 1   MANSIILPASSLLPFVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKS-ART 60

Query: 61  TDLVGLGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATR 120
           TDL GL  FT+NLAH +A ESRALA+ LA+K ADPK +ERYASC+  Y+DA S++ DA  
Sbjct: 61  TDLKGLITFTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKN 120

Query: 121 YLASGDYNGVNVEASAAMTNADDCEGNFT-APRPESELTKNSKTLEDICSIILVISNLLL 177
           YLA+GDY+GVN++AS AMT  DDC+ NFT +P   S L+K+ K LEDICSIILVI+N+LL
Sbjct: 121 YLATGDYDGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILL 180

[Close]

BLAST of Moc09g00890 vs. NCBI nr
Match: XP_038896410.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 196.1 bits (497), Expect = 3.9e-46
Identity = 110/182 (60.44%), Postives = 136/182 (74.73%), Query Frame = 0


Query: 1   MANSI-----SILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSA 60
           MANSI     S+LP VLSL+  + VVPM A SQN+ V++IC +T NP FC+ +LKS    
Sbjct: 1   MANSIILTASSLLPFVLSLLFFHHVVPMHAASQNNVVSIICQRTGNPPFCSNLLKS-ART 60

Query: 61  TDLVGLGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATR 120
           TDL GL  FTLNLA   A +SRALA+ LA K ADPKL+ERYASC++ Y+DA  +++DA  
Sbjct: 61  TDLKGLATFTLNLARTNATKSRALAQFLALKAADPKLKERYASCAEHYDDAAGDLDDAKN 120

Query: 121 YLASGDYNGVNVEASAAMTNADDCEGNFT-APRPESELTKNSKTLEDICSIILVISNLLL 177
           YLA+GDYNGVN++AS AMT  DDC+ NFT +P   S L+KN K LEDICSIILVI+N+LL
Sbjct: 121 YLATGDYNGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKNGKALEDICSIILVIANILL 180

[Close]

BLAST of Moc09g00890 vs. NCBI nr
Match: XP_022949197.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 187.2 bits (474), Expect = 1.8e-43
Identity = 101/157 (64.33%), Postives = 122/157 (77.71%), Query Frame = 0


Query: 20  VVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVGLGRFTLNLAHARAGESRAL 79
           +VPM A SQND V+ IC KTRNP FC  VLKS G+ TDL GL  FTLNLAH +A +S AL
Sbjct: 1   MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGT-TDLKGLATFTLNLAHDKATQSYAL 60

Query: 80  ARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASGDYNGVNVEASAAMTNADDC 139
           A+SLA+K  DPKL+ERYA+C++ Y+DAV +IED   YL  GDYNGVN++ASAAMT A+DC
Sbjct: 61  AQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDC 120

Query: 140 EGNFT-APRPESELTKNSKTLEDICSIILVISNLLLG 176
             +FT  P+  S L+ N KT+EDICSIILVI+NLLLG
Sbjct: 121 LDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG 156

[Close]

BLAST of Moc09g00890 vs. NCBI nr
Match: XP_023521203.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 186.4 bits (472), Expect = 3.1e-43
Identity = 104/179 (58.10%), Postives = 133/179 (74.30%), Query Frame = 0


Query: 1   MANS--ISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDL 60
           MA+S  +++  +VL ++  N +VPM A SQ+D V+ IC KTRNP FC  VLKS G+ TDL
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMQAASQDDIVSTICKKTRNPSFCFNVLKSAGT-TDL 60

Query: 61  VGLGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLA 120
            GL  FTLNLAH +  ++RALA+SLA+K  DPKL+ERYA+C++ Y+DA  +IED    L 
Sbjct: 61  KGLATFTLNLAHDKVAQTRALAQSLASKAVDPKLKERYATCAEQYDDAAGDIEDGKNNLG 120

Query: 121 SGDYNGVNVEASAAMTNADDCEGNFT-APRPESELTKNSKTLEDICSIILVISNLLLGR 177
            GDYNGVN++ASAAMT A DC  +FT  P+  S L+ N KT+EDICSIILVI+NLLLGR
Sbjct: 121 KGDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Moc09g00890 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 5.6e-27
Identity = 65/152 (42.76%), Postives = 98/152 (64.47%), Query Frame = 0


Query: 27  SQNDAVAMICPKTRNPGFCTYVLKS--TGSATDLVGLGRFTLNLAHARAGESRALARSLA 86
           ++N  ++ ICPKTRNP  C   L+S    ++ DL GLG+F++++A A A ++  +  SL 
Sbjct: 32  AENHLISEICPKTRNPSLCLQALESDPRSASKDLKGLGQFSIDIAQASAKQTSKIIASLT 91

Query: 87  AKTADPKLRERYASCSDSYNDAVSNIEDATRYLASGDYNGVNVEASAAMTNADDCEGNFT 146
            +  DPKL+ RY +CS++Y DA+ ++  A ++L SGDYN +N+ ASAA   A  CE +F 
Sbjct: 92  NQATDPKLKGRYETCSENYADAIDSLGQAKQFLTSGDYNSLNIYASAAFDGAGTCEDSFE 151

Query: 147 A-PRPESELTKNSKTLEDICSIILVISNLLLG 176
             P   ++L +    LED+C I+LVISNLL G
Sbjct: 152 GPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of Moc09g00890 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.6e-18
Identity = 61/174 (35.06%), Postives = 100/174 (57.47%), Query Frame = 0


Query: 1   MANSISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVG 60
           MA ++     + SL+ L  +    A++ ++ ++ IC KT NP FC   L +  ++ +L  
Sbjct: 1   MAANLRNNAFLSSLMFLLLIGSSYAITSSE-MSTICDKTLNPSFCLKFLNTKFASPNLQA 60

Query: 61  LGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASG 120
           L + TL+   ARA ++    +S+     DP+ +  Y SC D Y  A+ N+E+A  +LASG
Sbjct: 61  LAKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASG 120

Query: 121 DYNGVNVEASAAMTNADDCEGNFTAPRP-ESELTKNSKTLEDICSIILVISNLL 174
           D  G+N++ SAA+  AD C  +    R  +S +  NSKT++++C I LVISN+L
Sbjct: 121 DGMGMNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Moc09g00890 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.8e-17
Identity = 56/156 (35.90%), Postives = 81/156 (51.92%), Query Frame = 0


Query: 21  VPMGALSQNDA-VAMICPKTRNPGFCTYVLKST--GSATDLVGLGRFTLNLAHARAGESR 80
           V  G+L+   A +  IC K +N  FCT  +KS    S  DL  L   T   A   A E  
Sbjct: 18  VMTGSLNAQVADIKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGF 77

Query: 81  ALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASGDYNGVNVEASAAMTNAD 140
              +SL     +P +++ Y SC   Y  A+S++ DA + LASGD  G+N++ SAAM    
Sbjct: 78  RKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPS 137

Query: 141 DCEGNFTAPRPESELTKNSKTLEDICSIILVISNLL 174
            CE +    + +    KNS   ++IC I+LVISN++
Sbjct: 138 TCEQDMADFKVDPSAVKNSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Moc09g00890 vs. ExPASy TrEMBL
Match: A0A6J1CPI8 (pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111013386 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.3e-87
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MANSISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVG 60
           MANSISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVG
Sbjct: 1   MANSISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVG 60

Query: 61  LGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASG 120
           LGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASG
Sbjct: 61  LGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASG 120

Query: 121 DYNGVNVEASAAMTNADDCEGNFTAPRPESELTKNSKTLEDICSIILVISNLLLGR 177
           DYNGVNVEASAAMTNADDCEGNFTAPRPESELTKNSKTLEDICSIILVISNLLLGR
Sbjct: 121 DYNGVNVEASAAMTNADDCEGNFTAPRPESELTKNSKTLEDICSIILVISNLLLGR 176

[Close]

BLAST of Moc09g00890 vs. ExPASy TrEMBL
Match: A0A6J1GBD1 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452619 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 8.9e-44
Identity = 101/157 (64.33%), Postives = 122/157 (77.71%), Query Frame = 0


Query: 20  VVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVGLGRFTLNLAHARAGESRAL 79
           +VPM A SQND V+ IC KTRNP FC  VLKS G+ TDL GL  FTLNLAH +A +S AL
Sbjct: 1   MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGT-TDLKGLATFTLNLAHDKATQSYAL 60

Query: 80  ARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASGDYNGVNVEASAAMTNADDC 139
           A+SLA+K  DPKL+ERYA+C++ Y+DAV +IED   YL  GDYNGVN++ASAAMT A+DC
Sbjct: 61  AQSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDC 120

Query: 140 EGNFT-APRPESELTKNSKTLEDICSIILVISNLLLG 176
             +FT  P+  S L+ N KT+EDICSIILVI+NLLLG
Sbjct: 121 LDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG 156

[Close]

BLAST of Moc09g00890 vs. ExPASy TrEMBL
Match: A0A6J1EQC5 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111434861 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 3.4e-43
Identity = 106/179 (59.22%), Postives = 133/179 (74.30%), Query Frame = 0


Query: 1   MANS--ISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDL 60
           MA+S  +++  +VLS++  N V+PM A SQND  + IC KTRNP FC  VLKS G+ TDL
Sbjct: 1   MASSSFLAVSLVVLSVLFFNGVMPMHAASQNDVASTICKKTRNPSFCFDVLKSAGT-TDL 60

Query: 61  VGLGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLA 120
            GL  FTLNLAH +  ESRALA+SLA+K  DPKL+ERYA+C++ Y++A+S+IED    L 
Sbjct: 61  KGLATFTLNLAHDKTVESRALAQSLASKAIDPKLKERYATCAEQYDNAISDIEDGKVNLG 120

Query: 121 SGDYNGVNVEASAAMTNADDCEGNFT-APRPESELTKNSKTLEDICSIILVISNLLLGR 177
            GDYNGVN +ASAAMT A DC  +F   P+  S L+ N KT+EDICSIILVI+NLLLGR
Sbjct: 121 EGDYNGVNTKASAAMTEAGDCLDSFAQPPKDSSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Moc09g00890 vs. ExPASy TrEMBL
Match: A0A6J1ID39 (pectinesterase inhibitor-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471996 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 4.4e-43
Identity = 102/179 (56.98%), Postives = 134/179 (74.86%), Query Frame = 0


Query: 1   MANS--ISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDL 60
           MA+S  +++  +VL ++  N +VPM A SQ+D ++ IC KTRNP FC  VLKS G+ TDL
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDILSTICKKTRNPSFCFNVLKSAGT-TDL 60

Query: 61  VGLGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLA 120
             L  FTLNLAH +  ++RALA+SLA+K +DPKL+ERYA+C++ Y +A  +IED  + L 
Sbjct: 61  KRLATFTLNLAHDKVAQTRALAQSLASKASDPKLKERYATCAEQYGEAADDIEDGKKDLG 120

Query: 121 SGDYNGVNVEASAAMTNADDCEGNFT-APRPESELTKNSKTLEDICSIILVISNLLLGR 177
            GDYNGVN++ASAAMT A DC+ NFT  P+  S L+ N KT+EDICSIILVI+NLLLGR
Sbjct: 121 EGDYNGVNIKASAAMTEAGDCQDNFTQPPKDPSTLSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Moc09g00890 vs. ExPASy TrEMBL
Match: A0A6J1IDF4 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111475152 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 4.4e-43
Identity = 102/179 (56.98%), Postives = 134/179 (74.86%), Query Frame = 0


Query: 1   MANS--ISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDL 60
           MA+S  +++  +VL ++  N +VPM A SQ+D ++ IC KTRNP FC  VLKS G+ TDL
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDILSTICKKTRNPSFCFNVLKSAGT-TDL 60

Query: 61  VGLGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLA 120
             L  FTLNLAH +  ++RALA+SLA+K +DPKL+ERYA+C++ Y +A  +IED  + L 
Sbjct: 61  KRLATFTLNLAHDKVAQTRALAQSLASKASDPKLKERYATCAEQYGEAADDIEDGKKDLG 120

Query: 121 SGDYNGVNVEASAAMTNADDCEGNFT-APRPESELTKNSKTLEDICSIILVISNLLLGR 177
            GDYNGVN++ASAAMT A DC+ NFT  P+  S L+ N KT+EDICSIILVI+NLLLGR
Sbjct: 121 EGDYNGVNIKASAAMTEAGDCQDNFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of Moc09g00890 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 93.6 bits (231), Expect = 2.6e-19
Identity = 61/174 (35.06%), Postives = 100/174 (57.47%), Query Frame = 0


Query: 1   MANSISILPLVLSLVLLNTVVPMGALSQNDAVAMICPKTRNPGFCTYVLKSTGSATDLVG 60
           MA ++     + SL+ L  +    A++ ++ ++ IC KT NP FC   L +  ++ +L  
Sbjct: 1   MAANLRNNAFLSSLMFLLLIGSSYAITSSE-MSTICDKTLNPSFCLKFLNTKFASPNLQA 60

Query: 61  LGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASG 120
           L + TL+   ARA ++    +S+     DP+ +  Y SC D Y  A+ N+E+A  +LASG
Sbjct: 61  LAKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASG 120

Query: 121 DYNGVNVEASAAMTNADDCEGNFTAPRP-ESELTKNSKTLEDICSIILVISNLL 174
           D  G+N++ SAA+  AD C  +    R  +S +  NSKT++++C I LVISN+L
Sbjct: 121 DGMGMNMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Moc09g00890 vs. TAIR 10
Match: AT3G17220.1 (pectin methylesterase inhibitor 2 )

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-18
Identity = 56/156 (35.90%), Postives = 81/156 (51.92%), Query Frame = 0


Query: 21  VPMGALSQNDA-VAMICPKTRNPGFCTYVLKST--GSATDLVGLGRFTLNLAHARAGESR 80
           V  G+L+   A +  IC K +N  FCT  +KS    S  DL  L   T   A   A E  
Sbjct: 18  VMTGSLNAQVADIKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGF 77

Query: 81  ALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASGDYNGVNVEASAAMTNAD 140
              +SL     +P +++ Y SC   Y  A+S++ DA + LASGD  G+N++ SAAM    
Sbjct: 78  RKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPS 137

Query: 141 DCEGNFTAPRPESELTKNSKTLEDICSIILVISNLL 174
            CE +    + +    KNS   ++IC I+LVISN++
Sbjct: 138 TCEQDMADFKVDPSAVKNSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Moc09g00890 vs. TAIR 10
Match: AT1G48010.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.7e-07
Identity = 36/119 (30.25%), Postives = 66/119 (55.46%), Query Frame = 0


Query: 57  DLVGLGRFTLNLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRY 116
           DL G+ +  ++ A   A ++    + LA  T D + +E Y SCS  Y+ A+ + ++A + 
Sbjct: 57  DLPGVAKCLIHYAQQNALDTHNQLQLLANSTTDNRTKESYISCSKYYDKALYSFDEALKD 116

Query: 117 LASGDYNGVNVEASAAMTNADDCEGNFTAP--RPESELTKNSKTLEDICSIILVISNLL 174
           L   + + +N+E +AA  NA+DC+        +P+ +L      LE++C I+L IS++L
Sbjct: 117 LKVNNPDYLNIEITAARQNANDCKTLDLKDVFKPDPKLIMKIDFLENVCGILLSISDIL 175

[Close]

BLAST of Moc09g00890 vs. TAIR 10
Match: AT4G03945.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 5.2e-04
Identity = 41/171 (23.98%), Postives = 72/171 (42.11%), Query Frame = 0


Query: 10  LVLSLVLLNTVVPMGALSQNDAVAMICPKTR-NPGFCTYVLKSTG--SATDLVGLGRFTL 69
           LVL L + ++      L   D +  IC K   N  +C  VL +    +  D   L +F L
Sbjct: 15  LVLLLFVDSSYARFNTLVTKDQIHTICTKQDINSSYCFQVLNANPEIARLDFPSLFKFVL 74

Query: 70  NLAHARAGESRALARSLAAKTADPKLRERYASCSDSYNDAVSNIEDATRYLASGDYNGVN 129
           N  +     S  L +   +    P +  +Y  C + Y ++    ++A RYLA+ DY  VN
Sbjct: 75  N--YQAQNISDKLKQFKLSGGYTPGVESKYNLCIEFYENSFDRRDEALRYLAAKDYGSVN 134

Query: 130 VEASAAMTNADDCEGNFTAPRPESE-LTKNSKTLEDICSIILVISNLLLGR 177
                   N  +C  + +  +P  +     S  ++++  I+LVI    + +
Sbjct: 135 TMVGGTAINMFNCADDLSTMKPVPQFFVTESNLIKELSKILLVILECFISK 183

[Close]

BLAST of Moc09g00890 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 6.8e-04
Identity = 43/152 (28.29%), Postives = 61/152 (40.13%), Query Frame = 0


Query: 32  VAMICPKTRNPGFC--------TYVLKSTGSATDLVGLGRFTLNLAHARAGESRALARSL 91
           +  IC KT NP  C        T  LK   S+  ++                  A+  S+
Sbjct: 130 IKTICGKTDNPPLCESSVSPLLTPQLKPNTSSVLIL------------------AIQASI 189

Query: 92  AAKTADPKLRERY--ASCSDSYNDAVSNIEDATRYLASGDYNGVNVEASAAMTNADDCEG 151
            A  A   + E+   + C + Y+DAV N+EDA   + S D   VN   SAAMT+   C  
Sbjct: 190 TATKAAMAIVEKVDASDCQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCND 249

Query: 152 NFTAPRPESELTKNSKTLEDICSIILVISNLL 174
            F      + L   +  L  + S  L IS L+
Sbjct: 250 GFEESGEPNPLAYVADKLTKMVSNCLAISTLI 263

The following BLAST results are available for this feature:

BLAST of Moc09g00890 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143514.1	2.7e-87	100.00	pectinesterase inhibitor-like [Momordica charantia]	[more]
XP_038896411.1	3.0e-46	60.44	pectinesterase inhibitor-like [Benincasa hispida]	[more]
XP_038896410.1	3.9e-46	60.44	pectinesterase inhibitor-like [Benincasa hispida]	[more]
XP_022949197.1	1.8e-43	64.33	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
XP_023521203.1	3.1e-43	58.10	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc09g00890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	5.6e-27	42.76	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LNF2	3.6e-18	35.06	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]
Q9LUV1	1.8e-17	35.90	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

BLAST of Moc09g00890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CPI8	1.3e-87	100.00	pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111013386 PE=...	[more]
A0A6J1GBD1	8.9e-44	64.33	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452619 PE=4...	[more]
A0A6J1EQC5	3.4e-43	59.22	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111434861 PE=4...	[more]
A0A6J1ID39	4.4e-43	56.98	pectinesterase inhibitor-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1IDF4	4.4e-43	56.98	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111475152 PE=4 S...	[more]

BLAST of Moc09g00890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48020.1	2.6e-19	35.06	pectin methylesterase inhibitor 1	[more]
AT3G17220.1	1.3e-18	35.90	pectin methylesterase inhibitor 2	[more]
AT1G48010.1	1.7e-07	30.25	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G03945.1	5.2e-04	23.98	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G36659.1	6.8e-04	28.29	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 27..169 e-value: 5.9E-28 score: 108.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..173 e-value: 2.8E-32 score: 109.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 31..169 e-value: 1.0E-21 score: 77.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 27..174 e-value: 8.9E-35 score: 121.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 29..174
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 1..173
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 1..173
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 35..173 e-value: 3.26182E-46 score: 149.88

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g00890.1	Moc09g00890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity