Moc09g00660 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g00660
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRibonuclease T(2)
Locationchr9: 676343 .. 676747 (+)
RNA-Seq ExpressionMoc09g00660
SyntenyMoc09g00660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGAATACTGGCAGGACCTAGTCAGCGGAGACAACACCGCGGCTTGGCAGACCAAATGGGCAATCCACGGGACTTGCTCGGCCACGGTGTTTGACGAGTTCAAGTACTTCTGCATGGGTTTGGATACGTATGGCCGCCATGCTATCCTATCATTTCTGGTGCACGGCGGCCTGGTCCCGAGCTCTGCTCAACTGCACCACAAGACTAGCTTTATCAAAGCCATCGCGGACGTGACCTCGAAAAAGGGCGGCGTCATCTGTGCGGCCGACCAGCGGGGCCGAACACAGCTGAAGATGGTGGTTTTGTGTTATAGTAAAGATGGTGCAACAATGATCGACTGCCCTGATAATGTCAGTAGTTCCTGCCCTGATAAGTTCTTGTGGCTTGCCTTGGGTGATTGA

mRNA sequence

ATGGGCGAATACTGGCAGGACCTAGTCAGCGGAGACAACACCGCGGCTTGGCAGACCAAATGGGCAATCCACGGGACTTGCTCGGCCACGGTGTTTGACGAGTTCAAGTACTTCTGCATGGGTTTGGATACGTATGGCCGCCATGCTATCCTATCATTTCTGGTGCACGGCGGCCTGGTCCCGAGCTCTGCTCAACTGCACCACAAGACTAGCTTTATCAAAGCCATCGCGGACGTGACCTCGAAAAAGGGCGGCGTCATCTGTGCGGCCGACCAGCGGGGCCGAACACAGCTGAAGATGGTGGTTTTGTGTTATAGTAAAGATGGTGCAACAATGATCGACTGCCCTGATAATGTCAGTAGTTCCTGCCCTGATAAGTTCTTGTGGCTTGCCTTGGGTGATTGA

Coding sequence (CDS)

ATGGGCGAATACTGGCAGGACCTAGTCAGCGGAGACAACACCGCGGCTTGGCAGACCAAATGGGCAATCCACGGGACTTGCTCGGCCACGGTGTTTGACGAGTTCAAGTACTTCTGCATGGGTTTGGATACGTATGGCCGCCATGCTATCCTATCATTTCTGGTGCACGGCGGCCTGGTCCCGAGCTCTGCTCAACTGCACCACAAGACTAGCTTTATCAAAGCCATCGCGGACGTGACCTCGAAAAAGGGCGGCGTCATCTGTGCGGCCGACCAGCGGGGCCGAACACAGCTGAAGATGGTGGTTTTGTGTTATAGTAAAGATGGTGCAACAATGATCGACTGCCCTGATAATGTCAGTAGTTCCTGCCCTGATAAGTTCTTGTGGCTTGCCTTGGGTGATTGA

Protein sequence

MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVSSSCPDKFLWLALGD
Homology
BLAST of Moc09g00660 vs. NCBI nr
Match: XP_022143591.1 (ribonuclease 1-like [Momordica charantia])

HSP 1 Score: 288.1 bits (736), Expect = 3.9e-74
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0

Query: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60
           MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV
Sbjct: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60

Query: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120
           PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS
Sbjct: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120

Query: 121 SSCPDKFLWLALGD 135
           SSCPDKFLWLALGD
Sbjct: 121 SSCPDKFLWLALGD 134

BLAST of Moc09g00660 vs. NCBI nr
Match: XP_038899255.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 238.4 bits (607), Expect = 3.5e-59
Identity = 107/134 (79.85%), Postives = 120/134 (89.55%), Query Frame = 0

Query: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60
           M +YWQDL +G+   AW +KWAIHGTCSATVFDEFKYFC+GLDTYGRHA+LSFLVHGGLV
Sbjct: 88  MSQYWQDLENGNPIEAWASKWAIHGTCSATVFDEFKYFCLGLDTYGRHAMLSFLVHGGLV 147

Query: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120
           PSS++   KTSF+KAIAD+TSKKGGVICA DQRGRTQL+  VLCY+KDG T+IDCPDNVS
Sbjct: 148 PSSSKYLDKTSFVKAIADMTSKKGGVICAVDQRGRTQLQKAVLCYAKDGDTLIDCPDNVS 207

Query: 121 SSCPDKFLWLALGD 135
           SSCPD FLWLALGD
Sbjct: 208 SSCPDNFLWLALGD 221

BLAST of Moc09g00660 vs. NCBI nr
Match: XP_004149779.2 (ribonuclease MC [Cucumis sativus])

HSP 1 Score: 198.4 bits (503), Expect = 4.0e-47
Identity = 90/134 (67.16%), Postives = 105/134 (78.36%), Query Frame = 0

Query: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60
           M +YW+DL +G++  AW +KW +HGTCSA  FD+FKYFC+GLDTYGRHAI SFL   GL 
Sbjct: 88  MNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSFLDREGLA 147

Query: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120
           PSS++   K SFI AIA+ T KKGGVICA DQ  R QL+  VLCY KDG T+IDCPDNVS
Sbjct: 148 PSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLIDCPDNVS 207

Query: 121 SSCPDKFLWLALGD 135
           SSCPD F+WLALGD
Sbjct: 208 SSCPDNFVWLALGD 221

BLAST of Moc09g00660 vs. NCBI nr
Match: KGN45018.2 (hypothetical protein Csa_016393 [Cucumis sativus])

HSP 1 Score: 198.4 bits (503), Expect = 4.0e-47
Identity = 90/134 (67.16%), Postives = 105/134 (78.36%), Query Frame = 0

Query: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60
           M +YW+DL +G++  AW +KW +HGTCSA  FD+FKYFC+GLDTYGRHAI SFL   GL 
Sbjct: 87  MNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSFLDREGLA 146

Query: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120
           PSS++   K SFI AIA+ T KKGGVICA DQ  R QL+  VLCY KDG T+IDCPDNVS
Sbjct: 147 PSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLIDCPDNVS 206

Query: 121 SSCPDKFLWLALGD 135
           SSCPD F+WLALGD
Sbjct: 207 SSCPDNFVWLALGD 220

BLAST of Moc09g00660 vs. NCBI nr
Match: XP_022934593.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 95.9 bits (237), Expect = 2.8e-16
Identity = 46/133 (34.59%), Postives = 70/133 (52.63%), Query Frame = 0

Query: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHA--ILSFLVHGG 60
           +  YW D++ G N   WQ +W  HG CS   F++ +YF + L+    H+  +L+ L   G
Sbjct: 89  LNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYFQITLNIRTNHSYDLLTILNAAG 148

Query: 61  LVPSSAQLHHKTSFIKAIADVTSKKGGVIC--AADQRGRTQLKMVVLCYSKDGATMIDCP 120
           L PS   +        AI   T KK G+ C   A  + + QL  ++LC+ KDG T+IDC 
Sbjct: 149 LGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKEKKQLLEIILCFDKDGITLIDCT 208

Query: 121 DNVSSSCPDKFLW 130
           +    +CP +F+W
Sbjct: 209 EFAGITCPPQFVW 221

BLAST of Moc09g00660 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.2e-09
Identity = 35/116 (30.17%), Postives = 60/116 (51.72%), Query Frame = 0

Query: 5   WQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLVPSSA 64
           W +++  +N   W  +W  HGTCS + F++  YF + +D    + I+     G L P +A
Sbjct: 70  WPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDII-----GALRPHAA 129

Query: 65  QLHHKTSFIKAIADVTSKK----GGVICAADQRGR-TQLKMVVLCYSKDGATMIDC 116
             + +T   +AI      K     G+ C  D + + + L  VV C+++DG+T+IDC
Sbjct: 130 GPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

BLAST of Moc09g00660 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 61.2 bits (147), Expect = 1.0e-08
Identity = 37/121 (30.58%), Postives = 55/121 (45.45%), Query Frame = 0

Query: 5   WQDLVSGDNTAAWQTKWAIHGTCSAT--VFDEFKYFCMGLDTYGRHAILSFLVHGGLVPS 64
           W  L +G NT  W  +++ HGTCS T  + D   YF  G+  Y    I + L    + PS
Sbjct: 99  WPSL-TGPNTDFWTHEFSKHGTCSITGPITDIHDYFATGIKLYTEFNITAALESENIYPS 158

Query: 65  SAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVSSS 124
            +  +       AI      K G+ C++      QL  V +C  K+  +++DCPD    S
Sbjct: 159 DSNTYKPVDITNAITTHFGGKPGIQCSSG-----QLSTVAVCIDKNSLSIMDCPDLQGWS 213

BLAST of Moc09g00660 vs. ExPASy TrEMBL
Match: A0A6J1CQQ9 (ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111013449 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.9e-74
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0

Query: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60
           MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV
Sbjct: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60

Query: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120
           PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS
Sbjct: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120

Query: 121 SSCPDKFLWLALGD 135
           SSCPDKFLWLALGD
Sbjct: 121 SSCPDKFLWLALGD 134

BLAST of Moc09g00660 vs. ExPASy TrEMBL
Match: A0A0A0K4L1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G351900 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.0e-47
Identity = 90/134 (67.16%), Postives = 105/134 (78.36%), Query Frame = 0

Query: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60
           M +YW+DL +G++  AW +KW +HGTCSA  FD+FKYFC+GLDTYGRHAI SFL   GL 
Sbjct: 1   MNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSFLDREGLA 60

Query: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120
           PSS++   K SFI AIA+ T KKGGVICA DQ  R QL+  VLCY KDG T+IDCPDNVS
Sbjct: 61  PSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLIDCPDNVS 120

Query: 121 SSCPDKFLWLALGD 135
           SSCPD F+WLALGD
Sbjct: 121 SSCPDNFVWLALGD 134

BLAST of Moc09g00660 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.4e-16
Identity = 46/133 (34.59%), Postives = 70/133 (52.63%), Query Frame = 0

Query: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHA--ILSFLVHGG 60
           +  YW D++ G N   WQ +W  HG CS   F++ +YF + L+    H+  +L+ L   G
Sbjct: 89  LNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYFQITLNIRTNHSYDLLTILNAAG 148

Query: 61  LVPSSAQLHHKTSFIKAIADVTSKKGGVIC--AADQRGRTQLKMVVLCYSKDGATMIDCP 120
           L PS   +        AI   T KK G+ C   A  + + QL  ++LC+ KDG T+IDC 
Sbjct: 149 LGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKEKKQLLEIILCFDKDGITLIDCT 208

Query: 121 DNVSSSCPDKFLW 130
           +    +CP +F+W
Sbjct: 209 EFAGITCPPQFVW 221

BLAST of Moc09g00660 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.3e-16
Identity = 45/133 (33.83%), Postives = 73/133 (54.89%), Query Frame = 0

Query: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYF--CMGLDTYGRHAILSFLVHGG 60
           +  YW D++SG+N   W+ +W  HGTCS   F++ +YF   + + T   + +L+ L   G
Sbjct: 85  LNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAILNTAG 144

Query: 61  LVPSSAQLHHKTSFIKAIADVTSKKGGVICAAD-QRGRTQLKMVVLCYSKDGATMIDCPD 120
           L P++       +   AI   T KK G+ C  + Q  + QL  ++LC+ K+G T+IDC  
Sbjct: 145 LGPTATNTRQYGAIEGAIQAATGKKPGLRCNKNAQSKKKQLFEIILCFDKNGVTLIDCTQ 204

Query: 121 NVSSSCPDKFLWL 131
               +CP +F+WL
Sbjct: 205 FAGITCPSQFVWL 217

BLAST of Moc09g00660 vs. ExPASy TrEMBL
Match: A0A0A0LAN6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 5.2e-16
Identity = 47/126 (37.30%), Postives = 69/126 (54.76%), Query Frame = 0

Query: 5   WQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLVPSSA 64
           W DL  G+N   W  +W  HG CS   F  F+YF + L+   +  +L+ L   GL P ++
Sbjct: 91  WPDLKYGNNDKFWGHEWDTHGKCSDPPFSLFQYFQISLNLLRKFDLLTILKAAGLNPQTS 150

Query: 65  QLHHKTSFIKAIADVTSKKGGVICAAD-QRGRTQLKMVVLCYSKDGATMIDCPDNVSSSC 124
           Q     + +  I   T K  G+ C  + + G++QL  +VLC  KDGAT+IDCP  VS++C
Sbjct: 151 QNLAIQNIMAPIQRTTKKYPGIRCNKNVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTC 210

Query: 125 PDKFLW 130
              F+W
Sbjct: 211 AKSFVW 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143591.13.9e-74100.00ribonuclease 1-like [Momordica charantia][more]
XP_038899255.13.5e-5979.85ribonuclease MC-like [Benincasa hispida][more]
XP_004149779.24.0e-4767.16ribonuclease MC [Cucumis sativus][more]
KGN45018.24.0e-4767.16hypothetical protein Csa_016393 [Cucumis sativus][more]
XP_022934593.12.8e-1634.59ribonuclease MC-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P235401.2e-0930.17Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1[more]
Q7M4381.0e-0830.58Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1CQQ91.9e-74100.00ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111013449 PE=3 SV=1[more]
A0A0A0K4L12.0e-4767.16Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G351900 PE=3 SV=1[more]
A0A6J1F8371.4e-1634.59Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1[more]
A0A6J1EXD52.3e-1633.83Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1[more]
A0A0A0LAN65.2e-1637.30Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 1..115
e-value: 2.8E-17
score: 63.5
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 2..124
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 1..130
e-value: 1.3E-25
score: 92.7
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 1..129
NoneNo IPR availablePANTHERPTHR11240:SF49RIBONUCLEASE 3coord: 2..124
NoneNo IPR availableCDDcd00374RNase_T2coord: 1..126
e-value: 1.32277E-25
score: 93.6747

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g00660.1Moc09g00660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0016020 membrane
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding