Moc09g00520 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g00520
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr9: 536749 .. 540179 (+)
RNA-Seq ExpressionMoc09g00520
SyntenyMoc09g00520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCCAAAGAAATCCAATTTGCCAGTGATTACCTGATACTGAAGCCGGAGGAAGCAACTCTGCTGGATCTGTTTCTCTTCGTACTTCCTTTTGGTTTCAAAAATCTGAGGAAGTTGGTGGATTGTCCCCCCGAAAAAGAAGAGTCTTACAACTCCTTTGGAAACCGATGGATTATCTTCTTCTCCATTTTGTGGCAGAAAATCCTTCTCGCATTCTTAAATCAGCACAAAGGTATATTTTCATAACGAGGGTCGGGATAAAATTTTATGGAAAATACTTGGCTCCTTAATCTTTTCCTCACCATCTTTGACAACTATTTGACAACTAAGGGATAGAAATGAAAAAAATAGACTTAAAAGAAAAAAAAAAAACGAGTCTTTCTATGGTGGTGAAGACAAGATTTGATTCTTACTGATAAGGAGCCAAGTATTTTTCGAATTTTATTATACCCCTTTGGTAACATTAAAAAAACCTAAACATTTTGCAGTTAATTATATTTTTCTTATTTCTCATTTTTCGAAAACATCTTTAAAATTGTGTAGAAATTTTGAGAACGATCGGGCTCGTTTGGTAAGATTATCATTTCTTGTTTCTCGTTTTCAATTTTCTTAAAAAATCTAAACATTTTGCAACTAACTTTTTGTATAGAAATTTTGGAAACAACACAAACAAATTTTTTCTTTCTTGCTCTCATTTCTAAATTTGTTCATAAACATAAATAGAAAGGAAACATATTTTACCAAACAGACTCTAAGTTTCTTGTTTCTTGTTTTCGTCTTCAATTTGTTATAAAACATACATAATCATCTTTGAATTCATGAGTGTTGTGGCCTACAATTCTATTTTTATTGGTTAAAAATGAAGAAACAGAAATTAAAAAACGAAAACAGGTGTTTTAAGGACTGAGTTTTAGTTTTTAGTTAATTGAGTTTTGTTACGTGGGCATGCAGAAAAGCCAAAAACTGAGGATCCACCGACAGTGAACTGTAAAGATTGGAGTCTAAGTGGTCGGGATAAGAACATAAAATTAGACGACCCTTTCCAATATTATGCCATCCTCTCAGTCAGAGCTTCGGCTTTGGCGACCGAAGACTTTTCACCTCATCCTTCTGTGGTCGAGACCGTCGTCAATTCCTGCTGGAATGTAAATACAATATAATCCTTACCTCTCTCTCTTCATTTTTAATGATTTGGGTTACTAAAAAGATTCCTATAGATCGGTTAAGAGGTAAGAATTGTTAAGGTTTATGTGTTATTTAGCCGATTGATTATAATAACTAGGGGTATAAGCCTTTGGCTCGACTTAGTTTGGGAGTCAAATCGAAGCCGAACTGATAATAAACAACGTATTAACAAAAAAAATGAGTCGAATCGATAGTGATCAATATAAGATCGAGTCACGCTGAACTAACAAACCCGTCGGTTTATTGGTTTGGTCAGGCGGTTTGATTTCCACATGTCCTTGGATTTTAGGGTAGCAAACCTATTGCGTCGTACTACTTGGATCTACGGTTGGTTAGAAGAGATGGAGTGAATGGGAGGGAGGGAGGTGGGAGAGAGTCGGGAGGGATGGTTAGGAGAAAGATTCCTATTGATCGGTAAAGAAGCAAGTATAATAAGTTAATAACTCACTAGATAAAGACAGTTATTACTCCTCTTATTTCTTAATTGTTTAAAGAAAAATAAAATTGAGCTATATATGTTCGTACTAATATATCAGATTATTGTTATAAAGTAGTATAACAGATATAACTTCAATTATTATCCTCGTTGAATACTTGGGAATAGTGATGAGAACTAATGATCATGACGTTTTATTCTTTTCAATGCAGATGAAACTACTTGGATGCTACGATCTTTGGAATGGTAAGACTTTGGACTGTCACAGTCATAATAAATTTAATTAATTAATGGTCTTAAATTGATTTAACATAAATGAATGATTAATATTCTAAACATCCAATAACTTATTTTAAAATGAAATACCAATTAACTGTGACCATAATGAATCCATATTGCAAAAGACTGCCTATTAGTGGAGATGATTGTATGATTTGTGTAAGATACATAGTTTTTTTCTTTCCTTTTCTTTTATGCAACAAGTGAGGTGCGAAAAATGACAGGTCACGGTGACAATTATCGATAAACTAAGTTCATGTTTGTTGTTTTAAATTTGAACACGATGGTTTTCAGACTTCCAAGACATGGGTTCGACTCAAGTGTTTATGTTTCAGAATACTGCCACAGATCCAAACGTAACAGTGGTGGCATTTAGAGGCACCACTGACGTTTATGATATGAAGGTAGATTTGGACTTTTCCTGGTACGAGATTGCAAATGTGGGCAAAATCCACAGAGGGTTCATGGAAGCCCTTGGCCTGCAGAAGCAGCTAGGTTGGCCTAAGGAACTCGTAAAACCCGAGCACGATTATGCATATTACTTCCTCCGTCAACAACTCAGAGACATTGCCAAATCCAACGACAAGGCAAGGTTCATCATCACTGGCCATAGCTTGGGCGCAGCCCTCGCCACCCTGTTCGTCACCATCCTCGCCTTCCACGAGGAACTCGATTTGTTAGAGAAGTTAAAGGCTGTTTACACCTTTGGCCAACCCAGAGTCGGCGATGTCCAGTTTGTTCAGTTTATGGAAAACGTTAATTCAAAAGTACATAAATTCCCATATTACAGGTACGTTTACTCTATGGACGTGGTTCCCAGAATTCCTTTCGATGTCGAAGGTGATCAATGGTACAAACACTTTGGCGGATGTCTTTACTACGACTGCAACTACACTGGGGAGGTAATTATGAACCTTCGGCTGCAACCGCTCTTTTTCTCTCTTAATTAAATTATTATCCGAAGAGTATATTGGATTTGTAAAAAGAGTACAAACAAAGTTCTACATTGGTCAGGAGAGGGGACGTGGTCATGATATATAACAGAGGATAATTATCTTCATAGACATTTTAGGTGAAACCAAAAGTAAAATCGTAAGAATTATGCCCAAAGCAGACAATATCAATTAGTTTTTCAATAGAGGATGAGACGACTATTTTGTTACAATCATATTGAATAACTTCTCTTTTTTAGCTTTTTCGGGAGAGTAAACTAATGTTTTGGGTGCAATATACGCAGTTTCTGGAGGAGGAGCCAAACAAGAACTACTTGTCAATACCTTGGCTAATACCCAAATACTTGTACGCTTGGTGGGAGTTGCTAAGAAGTCTACTGCTCCCAGTAATCATGTGTAATCCGGATTACTTTGAGACTTCTCAGATTTTCTATGCCAGGGTTTTTGGGCTGTTTATTCCAGGAGCATCAGCTCACAACGTTGTGAACTATATTTACTCGGCCCGTTGGGGAAAAATAGTATGCAGAGACACCCAGAAAAAAGTAATTCCTGCAGACTATCTTGAACGCTGCTGA

mRNA sequence

ATGGGTTCCAAAGAAATCCAATTTGCCAGTGATTACCTGATACTGAAGCCGGAGGAAGCAACTCTGCTGGATCTGTTTCTCTTCGTACTTCCTTTTGGTTTCAAAAATCTGAGGAAGTTGGTGGATTGTCCCCCCGAAAAAGAAGAGTCTTACAACTCCTTTGGAAACCGATGGATTATCTTCTTCTCCATTTTGTGGCAGAAAATCCTTCTCGCATTCTTAAATCAGCACAAAGAAAAGCCAAAAACTGAGGATCCACCGACAGTGAACTGTAAAGATTGGAGTCTAAGTGGTCGGGATAAGAACATAAAATTAGACGACCCTTTCCAATATTATGCCATCCTCTCAGTCAGAGCTTCGGCTTTGGCGACCGAAGACTTTTCACCTCATCCTTCTGTGGTCGAGACCGTCGTCAATTCCTGCTGGAATATGAAACTACTTGGATGCTACGATCTTTGGAATGACTTCCAAGACATGGGTTCGACTCAAGTGTTTATGTTTCAGAATACTGCCACAGATCCAAACGTAACAGTGGTGGCATTTAGAGGCACCACTGACGTTTATGATATGAAGGTAGATTTGGACTTTTCCTGGTACGAGATTGCAAATGTGGGCAAAATCCACAGAGGGTTCATGGAAGCCCTTGGCCTGCAGAAGCAGCTAGGTTGGCCTAAGGAACTCGTAAAACCCGAGCACGATTATGCATATTACTTCCTCCGTCAACAACTCAGAGACATTGCCAAATCCAACGACAAGGCAAGGTTCATCATCACTGGCCATAGCTTGGGCGCAGCCCTCGCCACCCTGTTCGTCACCATCCTCGCCTTCCACGAGGAACTCGATTTGTTAGAGAAGTTAAAGGCTGTTTACACCTTTGGCCAACCCAGAGTCGGCGATGTCCAGTTTGTTCAGTTTATGGAAAACGTTAATTCAAAAGTACATAAATTCCCATATTACAGGTACGTTTACTCTATGGACGTGGTTCCCAGAATTCCTTTCGATGTCGAAGGTGATCAATGGTACAAACACTTTGGCGGATGTCTTTACTACGACTGCAACTACACTGGGGAGTTTCTGGAGGAGGAGCCAAACAAGAACTACTTGTCAATACCTTGGCTAATACCCAAATACTTGTACGCTTGGTGGGAGTTGCTAAGAAGTCTACTGCTCCCAGTAATCATGTGTAATCCGGATTACTTTGAGACTTCTCAGATTTTCTATGCCAGGGTTTTTGGGCTGTTTATTCCAGGAGCATCAGCTCACAACGTTGTGAACTATATTTACTCGGCCCGTTGGGGAAAAATAGTATGCAGAGACACCCAGAAAAAAGTAATTCCTGCAGACTATCTTGAACGCTGCTGA

Coding sequence (CDS)

ATGGGTTCCAAAGAAATCCAATTTGCCAGTGATTACCTGATACTGAAGCCGGAGGAAGCAACTCTGCTGGATCTGTTTCTCTTCGTACTTCCTTTTGGTTTCAAAAATCTGAGGAAGTTGGTGGATTGTCCCCCCGAAAAAGAAGAGTCTTACAACTCCTTTGGAAACCGATGGATTATCTTCTTCTCCATTTTGTGGCAGAAAATCCTTCTCGCATTCTTAAATCAGCACAAAGAAAAGCCAAAAACTGAGGATCCACCGACAGTGAACTGTAAAGATTGGAGTCTAAGTGGTCGGGATAAGAACATAAAATTAGACGACCCTTTCCAATATTATGCCATCCTCTCAGTCAGAGCTTCGGCTTTGGCGACCGAAGACTTTTCACCTCATCCTTCTGTGGTCGAGACCGTCGTCAATTCCTGCTGGAATATGAAACTACTTGGATGCTACGATCTTTGGAATGACTTCCAAGACATGGGTTCGACTCAAGTGTTTATGTTTCAGAATACTGCCACAGATCCAAACGTAACAGTGGTGGCATTTAGAGGCACCACTGACGTTTATGATATGAAGGTAGATTTGGACTTTTCCTGGTACGAGATTGCAAATGTGGGCAAAATCCACAGAGGGTTCATGGAAGCCCTTGGCCTGCAGAAGCAGCTAGGTTGGCCTAAGGAACTCGTAAAACCCGAGCACGATTATGCATATTACTTCCTCCGTCAACAACTCAGAGACATTGCCAAATCCAACGACAAGGCAAGGTTCATCATCACTGGCCATAGCTTGGGCGCAGCCCTCGCCACCCTGTTCGTCACCATCCTCGCCTTCCACGAGGAACTCGATTTGTTAGAGAAGTTAAAGGCTGTTTACACCTTTGGCCAACCCAGAGTCGGCGATGTCCAGTTTGTTCAGTTTATGGAAAACGTTAATTCAAAAGTACATAAATTCCCATATTACAGGTACGTTTACTCTATGGACGTGGTTCCCAGAATTCCTTTCGATGTCGAAGGTGATCAATGGTACAAACACTTTGGCGGATGTCTTTACTACGACTGCAACTACACTGGGGAGTTTCTGGAGGAGGAGCCAAACAAGAACTACTTGTCAATACCTTGGCTAATACCCAAATACTTGTACGCTTGGTGGGAGTTGCTAAGAAGTCTACTGCTCCCAGTAATCATGTGTAATCCGGATTACTTTGAGACTTCTCAGATTTTCTATGCCAGGGTTTTTGGGCTGTTTATTCCAGGAGCATCAGCTCACAACGTTGTGAACTATATTTACTCGGCCCGTTGGGGAAAAATAGTATGCAGAGACACCCAGAAAAAAGTAATTCCTGCAGACTATCTTGAACGCTGCTGA

Protein sequence

MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWIIFFSILWQKILLAFLNQHKEKPKTEDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDTQKKVIPADYLERC
Homology
BLAST of Moc09g00520 vs. NCBI nr
Match: XP_022152547.1 (uncharacterized protein LOC111020243 [Momordica charantia])

HSP 1 Score: 960.3 bits (2481), Expect = 5.9e-276
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII
Sbjct: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60

Query: 61  FFSILWQKILLAFLNQHKEKPKTEDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRAS 120
           FFSILWQKILLAFLNQHKEKPKTEDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRAS
Sbjct: 61  FFSILWQKILLAFLNQHKEKPKTEDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRAS 120

Query: 121 ALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVA 180
           ALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVA
Sbjct: 121 ALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVA 180

Query: 181 FRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLR 240
           FRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLR
Sbjct: 181 FRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLR 240

Query: 241 QQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDV 300
           QQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDV
Sbjct: 241 QQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDV 300

Query: 301 QFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLE 360
           QFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLE
Sbjct: 301 QFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLE 360

Query: 361 EEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASA 420
           EEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASA
Sbjct: 361 EEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASA 420

Query: 421 HNVVNYIYSARWGKIVCRDTQKKVIPADYLERC 454
           HNVVNYIYSARWGKIVCRDTQKKVIPADYLERC
Sbjct: 421 HNVVNYIYSARWGKIVCRDTQKKVIPADYLERC 453

BLAST of Moc09g00520 vs. NCBI nr
Match: XP_022139747.1 (uncharacterized protein LOC111010582 [Momordica charantia])

HSP 1 Score: 612.5 bits (1578), Expect = 3.0e-171
Identity = 294/457 (64.33%), Postives = 347/457 (75.93%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MGS+E +F+SDYLILKPEEATLL LFLF LPFG++ LR LVDCP  KE SY +FGNRW+I
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 61  FFSILWQKILLAFLNQHK-------EKPKTEDPPTVNCKDWSLSGRDKNIKL--DDPFQY 120
           F SIL QK LLA  N  K       +  KT   P VNCK+W + G +KNIKL  DD F+Y
Sbjct: 61  FISILLQKFLLAIANLIKYCKSMRGKFHKTCAAPKVNCKEWKIIGMEKNIKLADDDTFKY 120

Query: 121 YAILSVRASALATEDF-SPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNT 180
           Y  L+V AS LA +D+ SP PSVV+TVVN CW M LLGCYD WNDFQ+  +TQVFMFQNT
Sbjct: 121 YGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKMNLLGCYDFWNDFQNKATTQVFMFQNT 180

Query: 181 ATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKP 240
           ATDPNVTVVAFRG++++YD  VDL+ SWY I  +GKIH GFM+ALGLQK  GWPKEL  P
Sbjct: 181 ATDPNVTVVAFRGSSEIYDWMVDLNLSWYNIEGIGKIHGGFMQALGLQKGTGWPKELANP 240

Query: 241 EHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVY 300
           +HD+AYY LRQQLRDI KSND ARFI TGHSLG ALA LF T+LAFH++  LLEKL+AVY
Sbjct: 241 DHDFAYYTLRQQLRDIVKSNDNARFIFTGHSLGGALAVLFATLLAFHDDATLLEKLQAVY 300

Query: 301 TFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYY 360
           TFGQPRVGD +F QFMEN   K + F YYRYVYS D+VPR+PFD   + WY+HFGGC+Y+
Sbjct: 301 TFGQPRVGDRRFAQFMENTIEK-YGFKYYRYVYSSDLVPRVPFDFNDNLWYEHFGGCVYF 360

Query: 361 DCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARV 420
           +C+Y G+FLE +PNKNY S  WLI KY+ AWWEL+RSL++PVI C+ DY E       R+
Sbjct: 361 NCSYNGKFLEVQPNKNYFSPIWLISKYVSAWWELIRSLMIPVIKCSSDYLEGFSNLLLRL 420

Query: 421 FGLFIPGASAHNVVNYIYSARWGKIVCRDTQKKVIPA 448
           FGL  PGASAH  +NYI S RWGK    + +K +IPA
Sbjct: 421 FGLLFPGASAHMCLNYINSIRWGKC---NHEKTLIPA 453

BLAST of Moc09g00520 vs. NCBI nr
Match: XP_022135571.1 (uncharacterized protein LOC111007483 isoform X2 [Momordica charantia])

HSP 1 Score: 578.9 bits (1491), Expect = 3.7e-161
Identity = 294/466 (63.09%), Postives = 350/466 (75.11%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MG ++ QF++DYLILKPEEATLLDLFLF+LPFG+  LRKLVDCP EKEESY SFGNRW+I
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 61  FFSILWQKILLAFLN-QHKEKPKTED-------PPTVNCKDWSLSGRDKNIKL---DDPF 120
           F SIL QK LLA  N   K +P              VNC+DW + G+++NIKL   DD F
Sbjct: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVQTYASEVNCRDWKI-GKEENIKLLNDDDTF 120

Query: 121 QYYAILSVRASALATEDFS-PHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQ 180
            YY  ++V AS L  +D S P  SVV TVVNSCW MKLLGCYD  N F+    TQ  MFQ
Sbjct: 121 VYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCYDFENMFESKARTQATMFQ 180

Query: 181 NTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEI-ANVGKIHRGFMEALGLQK-QLGWPKE 240
           NTATDPNVTVVAF+GT  +YD  +DL+FSWY +   +G IH GFMEALGLQK  +GWPKE
Sbjct: 181 NTATDPNVTVVAFKGTNTIYDAILDLNFSWYNVDQRIGNIHSGFMEALGLQKGTIGWPKE 240

Query: 241 LVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKL 300
           L K +H +AYY+LRQQLRDIAKSNDKA+FI TGHSLG ALA LFVTIL++H E D+L KL
Sbjct: 241 LPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAILFVTILSYHNESDVLNKL 300

Query: 301 KAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGG 360
           +AVYTFGQPRVG+ QFV+FME+  SK + F YYRYVYSMD+VPRIPFD+  D+WYKHFGG
Sbjct: 301 QAVYTFGQPRVGNSQFVKFMES-TSKQYAFKYYRYVYSMDLVPRIPFDI-FDEWYKHFGG 360

Query: 361 CLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIF 420
           C+Y++C Y+G+F+E +PNKNY S  WLIPKYL+A WEL+RSL++P+I  +  YFE  +  
Sbjct: 361 CVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELIRSLMMPLIKFSLYYFEGFKTL 420

Query: 421 YARVFGLFIPGASAHNVVNYIYSARWGKI-VCRDTQKKVIPADYLE 452
             R FGL IPGASAH  VNYIY AR GK+   +D QK +I  D LE
Sbjct: 421 LLRFFGLLIPGASAHLCVNYIYLARQGKMPPLKDIQKTLISEDGLE 463

BLAST of Moc09g00520 vs. NCBI nr
Match: XP_022135562.1 (uncharacterized protein LOC111007483 isoform X1 [Momordica charantia])

HSP 1 Score: 574.7 bits (1480), Expect = 7.0e-160
Identity = 295/468 (63.03%), Postives = 351/468 (75.00%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MGS E +F++DYL LKP+EATLLDLF+F+LPFG   LRKLVDCPPEKEESY SF NRW+I
Sbjct: 1   MGSLEYEFSNDYLKLKPKEATLLDLFIFLLPFGSIKLRKLVDCPPEKEESYTSFTNRWVI 60

Query: 61  FFSILWQKILLAFLN------QHKEKPKTEDPPT----VNCKDWSLSGRDKNIKL---DD 120
           FFSIL QK L A  N         EK +T  P T    VNC+DW + G+++NIKL   DD
Sbjct: 61  FFSILSQKSLFALANLLEKWESSTEKLRTA-PQTYASEVNCRDWKI-GKEENIKLLNDDD 120

Query: 121 PFQYYAILSVRASALATEDFS-PHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFM 180
            F YY  ++V AS L  +D S P  SVV TVVNSCW MKLLGCYD  N F+    TQ  M
Sbjct: 121 TFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCYDFENMFESKARTQATM 180

Query: 181 FQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEI-ANVGKIHRGFMEALGLQK-QLGWP 240
           FQNTATDPNVTVVAF+GT  +YD  +DL+FSWY +   +G IH GFMEALGLQK  +GWP
Sbjct: 181 FQNTATDPNVTVVAFKGTNTIYDAILDLNFSWYNVDQRIGNIHSGFMEALGLQKGTIGWP 240

Query: 241 KELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLE 300
           KEL K +H +AYY+LRQQLRDIAKSNDKA+FI TGHSLG ALA LFVTIL++H E D+L 
Sbjct: 241 KELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAILFVTILSYHNESDVLN 300

Query: 301 KLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHF 360
           KL+AVYTFGQPRVG+ QFV+FME+  SK + F YYRYVYSMD+VPRIPFD+  D+WYKHF
Sbjct: 301 KLQAVYTFGQPRVGNSQFVKFMES-TSKQYAFKYYRYVYSMDLVPRIPFDI-FDEWYKHF 360

Query: 361 GGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQ 420
           GGC+Y++C Y+G+F+E +PNKNY S  WLIPKYL+A WEL+RSL++P+I  +  YFE  +
Sbjct: 361 GGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELIRSLMMPLIKFSLYYFEGFK 420

Query: 421 IFYARVFGLFIPGASAHNVVNYIYSARWGKI-VCRDTQKKVIPADYLE 452
               R FGL IPGASAH  VNYIY AR GK+   +D QK +I  D LE
Sbjct: 421 TLLLRFFGLLIPGASAHLCVNYIYLARQGKMPPLKDIQKTLISEDGLE 464

BLAST of Moc09g00520 vs. NCBI nr
Match: XP_022132571.1 (uncharacterized protein LOC111005368 isoform X1 [Momordica charantia])

HSP 1 Score: 525.4 bits (1352), Expect = 4.9e-145
Identity = 264/451 (58.54%), Postives = 324/451 (71.84%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MGS++ QF+ DYLILKPEEATLLDLFLF+LPFGF+ +RKLVDCP  KE SY S  +R II
Sbjct: 1   MGSEDHQFSDDYLILKPEEATLLDLFLFILPFGFRKIRKLVDCPAAKENSYRSIQSRLII 60

Query: 61  FFSILWQKILLAFLNQHKE-------------KPKTEDPPTVNCKDWSLSGRDKNIKL-D 120
           F SIL+QK++LA  N  K+             KP     P VNC+DW + G +KN+KL +
Sbjct: 61  FVSILFQKLVLAISNLAKKFKLMRGKLWAVSYKPYA---PKVNCRDWKIGG-EKNVKLPE 120

Query: 121 DPFQYYAILSVRASALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFM 180
           D F+YY+ L+V AS LA  D+SP P++V+TVV  CW M L+  Y   N F +   T  FM
Sbjct: 121 DNFKYYSALTVMASQLAYTDYSPQPNIVQTVVEGCWKMNLINTYKFRNGFIEKARTCAFM 180

Query: 181 FQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKE 240
           FQ T+TDPNV ++AF+GT+   D  VD++FSW  I  VG+IH GFM+ALGLQ+   WP E
Sbjct: 181 FQTTSTDPNVIILAFKGTSQCCDCAVDVNFSWCSIPGVGRIHMGFMQALGLQEAFHWPDE 240

Query: 241 LVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKL 300
           L KP  D+AYY LRQQLRDIAKSND ARFI TGHSLG ALA LFVTILA+H E D+LEKL
Sbjct: 241 LPKPNSDFAYYTLRQQLRDIAKSNDNARFIFTGHSLGGALAVLFVTILAYHNESDVLEKL 300

Query: 301 KAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQW--YKHF 360
           +AVYTFGQPRVGD +F QFMEN   K + F YYRYVYS D+V RIPF+  GD +  YKHF
Sbjct: 301 QAVYTFGQPRVGDPKFAQFMENTTKK-YDFKYYRYVYSFDLVSRIPFNC-GDFFCSYKHF 360

Query: 361 GGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQ 420
           GGC+Y+DC Y G+FLEE+PN NY    +LIP++L A WE +RSL++P++ C+ DYFE   
Sbjct: 361 GGCVYFDCCYNGKFLEEQPNNNYYWPIYLIPQFLAALWEFIRSLMIPLVKCSWDYFEGFG 420

Query: 421 IFYARVFGLFIPGASAHNVVNYIYSARWGKI 436
               RV GLFIP  SAH ++NYI S R+GKI
Sbjct: 421 NLKNRVLGLFIPATSAHMLLNYINSTRYGKI 445

BLAST of Moc09g00520 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 7.5e-64
Identity = 171/500 (34.20%), Postives = 248/500 (49.60%), Query Frame = 0

Query: 10  SDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEES-----YNSFGNRWIIFFSI 69
           S+YLI++P     +DLF + +     +  K ++ P  +E S       +  +RW+I  SI
Sbjct: 17  SNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWVIVVSI 76

Query: 70  LWQKI------------------------------LLAFLNQHK----EKPKTEDPPTVN 129
           L +KI                              LL  L Q K    E+       T+ 
Sbjct: 77  LVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTFVSTIG 136

Query: 130 --------CKDWS----LSGRDK----NIKLDDPFQYYAILSVRASALATEDFSPHPSVV 189
                    K+W+    L G D      +K++   +    L V AS LA E    +  VV
Sbjct: 137 QLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYE----NAKVV 196

Query: 190 ETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTT--DVYDMKV 249
           E VV+  W M L+   D WND+Q   STQVF+F +   D N+ V++FRGT   D  D   
Sbjct: 197 ENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWGT 256

Query: 250 DLDFSWYEIANVGKIHRGFMEALGL------------------QKQLGWPKELVKPEHDY 309
           D D+SWYE+ NVGK+H GF+EA+GL                   ++    K L+      
Sbjct: 257 DFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVERS 316

Query: 310 AYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQ 369
           AYY +R  L+ +   ++ ARF++TGHSLG ALA LF T+L  +EE +++++L  VYTFGQ
Sbjct: 317 AYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQ 376

Query: 370 PRVGDVQFVQFME-NVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCN 429
           PR+G+ +   FM+  +N  V +  Y+R VY  D+VPR+P+D +    YKHFG CL+YD  
Sbjct: 377 PRIGNREVGLFMKAQLNQPVDR--YFRVVYCNDIVPRLPYD-DKTFLYKHFGLCLFYDSF 436

Query: 430 YTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGL 434
           Y     E+EP+ N   + + I  ++ A WEL+R L +      PDY E       R+ GL
Sbjct: 437 YNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTH-GPDYKEGWFRILFRLMGL 496

BLAST of Moc09g00520 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.1e-62
Identity = 147/389 (37.79%), Postives = 201/389 (51.67%), Query Frame = 0

Query: 115 LSVRASALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDP 174
           L + AS LA E    +  VV  VVN  W M  +  Y+ WNDF+   STQVF+  +   D 
Sbjct: 190 LCMMASKLAYE----NEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDA 249

Query: 175 NVTVVAFRGTT--DVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGW--------- 234
           N+ +V+FRGT   D  D   D D+SWYEI  +GK+H GF+EALGL  +            
Sbjct: 250 NLILVSFRGTEPFDADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFV 309

Query: 235 ----------------PKELVKPEHDY--------------------------------- 294
                           P E  K    +                                 
Sbjct: 310 NNLKFANLENVHATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPER 369

Query: 295 -AYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFG 354
            AYY +R +L+ + K +  A+F++TGHSLG ALA LF  +L  HEE+D++E+L  +YT+G
Sbjct: 370 TAYYVVRSKLKRLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYG 429

Query: 355 QPRVGDVQFVQFME-NVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDC 414
           QPRVG+ Q  +FME ++   V K  Y+R VY  D+VPR+P+D      +KHFG C YY+ 
Sbjct: 430 QPRVGNRQLGRFMEAHLEHPVPK--YFRVVYCNDLVPRLPYD-NKTFLFKHFGICQYYNS 489

Query: 415 NYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFG 442
            Y  + + EEPN NY  + +L+P YL A WEL+RS  +   M   +Y E  +    R  G
Sbjct: 490 LYIEQNINEEPNPNYFGMRFLVPLYLNAGWELIRSFTMG-YMYGSEYEECWESVMLRALG 549

BLAST of Moc09g00520 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.0e-56
Identity = 132/346 (38.15%), Postives = 188/346 (54.34%), Query Frame = 0

Query: 115 LSVRASALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDP 174
           L + AS LA E    +  VVE VV   W M  +  Y   N FQD  +T  F+F +   D 
Sbjct: 172 LCIMASKLAYE----NTKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDA 231

Query: 175 NVTVVAFRGT--TDVYDMKVDLDFSWYEIANVGKIHRGFMEALGL--------------Q 234
           N+ V++FRGT    + +   D DFS   + + G +H GF+EA+GL               
Sbjct: 232 NLIVISFRGTGPFSIPNWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINT 291

Query: 235 KQLGWPKELVK-----PEH-----DYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALAT 294
           K  G   EL K     P+H     D  Y+     L+ + K +  A+F++TGHSLG ALA 
Sbjct: 292 KSPGSITELRKESEMAPDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAI 351

Query: 295 LFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVV 354
           LF  IL   +E ++L++L  VYTFGQPR+G+     FM+N      +  Y+R VY  D+V
Sbjct: 352 LFTCILEIQQETEVLDRLLNVYTFGQPRIGNYNLGYFMQN-RLNFPERRYFRVVYCNDMV 411

Query: 355 PRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSL 414
           PR+PFD +    ++HFG C+YYD  + G F +EEP++N   I   I  ++ AWWEL RS 
Sbjct: 412 PRVPFD-DVFFTFEHFGTCIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSF 471

Query: 415 LLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGK 435
           +L  +    +Y ET +    R+ GLF+PG +AH+ VNY+ S R G+
Sbjct: 472 ILNHVY-GAEYKETWESRMFRILGLFLPGVAAHSPVNYVNSVRLGR 510

BLAST of Moc09g00520 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-07
Identity = 47/156 (30.13%), Postives = 69/156 (44.23%), Query Frame = 0

Query: 179 VAFRGTTDVYDMKVDLDF--SWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAY 238
           + FRG++ + +   DL F    Y   +  K+H+GF+++ G  +      ELV        
Sbjct: 171 IVFRGSSSIRNWIADLTFVPVSYPPVSGTKVHKGFLDSYGEVQ-----NELV-------- 230

Query: 239 YFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPR 298
                 + D  K     +  +TGHSLG A A L    L +  E  L      +YT GQPR
Sbjct: 231 ----ATVLDQFKQYPSYKVAVTGHSLGGATALLCALDL-YQREEGLSSSNLFLYTQGQPR 290

Query: 299 VGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIP 333
           VGD  F  ++ +        PY R V   D+VP +P
Sbjct: 291 VGDPAFANYVVSTG-----IPYRRTVNERDIVPHLP 303

BLAST of Moc09g00520 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-07
Identity = 60/238 (25.21%), Postives = 104/238 (43.70%), Query Frame = 0

Query: 99  RDKNIKLDDPFQYYAILSVRASALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQD 158
           R+ +  L + F  +A  S  A      D +P  + +    N+C  ++      L++ F+D
Sbjct: 22  REVSQDLFNQFNLFAQYSAAAYCGKNND-APAGTNITCTGNACPEVEKADATFLYS-FED 81

Query: 159 MGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIANV---GKIHRGFMEAL 218
            G   V  F        + V++FRG+  + +   +L+F   EI ++    + H GF  + 
Sbjct: 82  SGVGDVTGFLALDNTNKLIVLSFRGSRSIENWIGNLNFDLKEINDICSGCRGHDGFTSS- 141

Query: 219 GLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILA 278
                  W               LRQ++ D  + +   R + TGHSLG ALAT+    L 
Sbjct: 142 -------WRS---------VADTLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLR 201

Query: 279 FH-EELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIP 333
            +  ++D       V+++G PRVG+  F +F+            YR  ++ D+VPR+P
Sbjct: 202 GNGYDID-------VFSYGAPRVGNRAFAEFLTVQTGGT----LYRITHTNDIVPRLP 229

BLAST of Moc09g00520 vs. ExPASy TrEMBL
Match: A0A6J1DGJ7 (uncharacterized protein LOC111020243 OS=Momordica charantia OX=3673 GN=LOC111020243 PE=4 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 2.9e-276
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII
Sbjct: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60

Query: 61  FFSILWQKILLAFLNQHKEKPKTEDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRAS 120
           FFSILWQKILLAFLNQHKEKPKTEDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRAS
Sbjct: 61  FFSILWQKILLAFLNQHKEKPKTEDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRAS 120

Query: 121 ALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVA 180
           ALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVA
Sbjct: 121 ALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVA 180

Query: 181 FRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLR 240
           FRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLR
Sbjct: 181 FRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLR 240

Query: 241 QQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDV 300
           QQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDV
Sbjct: 241 QQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDV 300

Query: 301 QFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLE 360
           QFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLE
Sbjct: 301 QFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLE 360

Query: 361 EEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASA 420
           EEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASA
Sbjct: 361 EEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASA 420

Query: 421 HNVVNYIYSARWGKIVCRDTQKKVIPADYLERC 454
           HNVVNYIYSARWGKIVCRDTQKKVIPADYLERC
Sbjct: 421 HNVVNYIYSARWGKIVCRDTQKKVIPADYLERC 453

BLAST of Moc09g00520 vs. ExPASy TrEMBL
Match: A0A6J1CEU2 (uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010582 PE=4 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.5e-171
Identity = 294/457 (64.33%), Postives = 347/457 (75.93%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MGS+E +F+SDYLILKPEEATLL LFLF LPFG++ LR LVDCP  KE SY +FGNRW+I
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 61  FFSILWQKILLAFLNQHK-------EKPKTEDPPTVNCKDWSLSGRDKNIKL--DDPFQY 120
           F SIL QK LLA  N  K       +  KT   P VNCK+W + G +KNIKL  DD F+Y
Sbjct: 61  FISILLQKFLLAIANLIKYCKSMRGKFHKTCAAPKVNCKEWKIIGMEKNIKLADDDTFKY 120

Query: 121 YAILSVRASALATEDF-SPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNT 180
           Y  L+V AS LA +D+ SP PSVV+TVVN CW M LLGCYD WNDFQ+  +TQVFMFQNT
Sbjct: 121 YGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKMNLLGCYDFWNDFQNKATTQVFMFQNT 180

Query: 181 ATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKP 240
           ATDPNVTVVAFRG++++YD  VDL+ SWY I  +GKIH GFM+ALGLQK  GWPKEL  P
Sbjct: 181 ATDPNVTVVAFRGSSEIYDWMVDLNLSWYNIEGIGKIHGGFMQALGLQKGTGWPKELANP 240

Query: 241 EHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVY 300
           +HD+AYY LRQQLRDI KSND ARFI TGHSLG ALA LF T+LAFH++  LLEKL+AVY
Sbjct: 241 DHDFAYYTLRQQLRDIVKSNDNARFIFTGHSLGGALAVLFATLLAFHDDATLLEKLQAVY 300

Query: 301 TFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYY 360
           TFGQPRVGD +F QFMEN   K + F YYRYVYS D+VPR+PFD   + WY+HFGGC+Y+
Sbjct: 301 TFGQPRVGDRRFAQFMENTIEK-YGFKYYRYVYSSDLVPRVPFDFNDNLWYEHFGGCVYF 360

Query: 361 DCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARV 420
           +C+Y G+FLE +PNKNY S  WLI KY+ AWWEL+RSL++PVI C+ DY E       R+
Sbjct: 361 NCSYNGKFLEVQPNKNYFSPIWLISKYVSAWWELIRSLMIPVIKCSSDYLEGFSNLLLRL 420

Query: 421 FGLFIPGASAHNVVNYIYSARWGKIVCRDTQKKVIPA 448
           FGL  PGASAH  +NYI S RWGK    + +K +IPA
Sbjct: 421 FGLLFPGASAHMCLNYINSIRWGKC---NHEKTLIPA 453

BLAST of Moc09g00520 vs. ExPASy TrEMBL
Match: A0A6J1C148 (uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.8e-161
Identity = 294/466 (63.09%), Postives = 350/466 (75.11%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MG ++ QF++DYLILKPEEATLLDLFLF+LPFG+  LRKLVDCP EKEESY SFGNRW+I
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 61  FFSILWQKILLAFLN-QHKEKPKTED-------PPTVNCKDWSLSGRDKNIKL---DDPF 120
           F SIL QK LLA  N   K +P              VNC+DW + G+++NIKL   DD F
Sbjct: 61  FISILSQKFLLAIANLLEKWEPMRGKFWRVQTYASEVNCRDWKI-GKEENIKLLNDDDTF 120

Query: 121 QYYAILSVRASALATEDFS-PHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQ 180
            YY  ++V AS L  +D S P  SVV TVVNSCW MKLLGCYD  N F+    TQ  MFQ
Sbjct: 121 VYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCYDFENMFESKARTQATMFQ 180

Query: 181 NTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEI-ANVGKIHRGFMEALGLQK-QLGWPKE 240
           NTATDPNVTVVAF+GT  +YD  +DL+FSWY +   +G IH GFMEALGLQK  +GWPKE
Sbjct: 181 NTATDPNVTVVAFKGTNTIYDAILDLNFSWYNVDQRIGNIHSGFMEALGLQKGTIGWPKE 240

Query: 241 LVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKL 300
           L K +H +AYY+LRQQLRDIAKSNDKA+FI TGHSLG ALA LFVTIL++H E D+L KL
Sbjct: 241 LPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAILFVTILSYHNESDVLNKL 300

Query: 301 KAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGG 360
           +AVYTFGQPRVG+ QFV+FME+  SK + F YYRYVYSMD+VPRIPFD+  D+WYKHFGG
Sbjct: 301 QAVYTFGQPRVGNSQFVKFMES-TSKQYAFKYYRYVYSMDLVPRIPFDI-FDEWYKHFGG 360

Query: 361 CLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIF 420
           C+Y++C Y+G+F+E +PNKNY S  WLIPKYL+A WEL+RSL++P+I  +  YFE  +  
Sbjct: 361 CVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELIRSLMMPLIKFSLYYFEGFKTL 420

Query: 421 YARVFGLFIPGASAHNVVNYIYSARWGKI-VCRDTQKKVIPADYLE 452
             R FGL IPGASAH  VNYIY AR GK+   +D QK +I  D LE
Sbjct: 421 LLRFFGLLIPGASAHLCVNYIYLARQGKMPPLKDIQKTLISEDGLE 463

BLAST of Moc09g00520 vs. ExPASy TrEMBL
Match: A0A6J1C321 (uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 3.4e-160
Identity = 295/468 (63.03%), Postives = 351/468 (75.00%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MGS E +F++DYL LKP+EATLLDLF+F+LPFG   LRKLVDCPPEKEESY SF NRW+I
Sbjct: 1   MGSLEYEFSNDYLKLKPKEATLLDLFIFLLPFGSIKLRKLVDCPPEKEESYTSFTNRWVI 60

Query: 61  FFSILWQKILLAFLN------QHKEKPKTEDPPT----VNCKDWSLSGRDKNIKL---DD 120
           FFSIL QK L A  N         EK +T  P T    VNC+DW + G+++NIKL   DD
Sbjct: 61  FFSILSQKSLFALANLLEKWESSTEKLRTA-PQTYASEVNCRDWKI-GKEENIKLLNDDD 120

Query: 121 PFQYYAILSVRASALATEDFS-PHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFM 180
            F YY  ++V AS L  +D S P  SVV TVVNSCW MKLLGCYD  N F+    TQ  M
Sbjct: 121 TFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCYDFENMFESKARTQATM 180

Query: 181 FQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEI-ANVGKIHRGFMEALGLQK-QLGWP 240
           FQNTATDPNVTVVAF+GT  +YD  +DL+FSWY +   +G IH GFMEALGLQK  +GWP
Sbjct: 181 FQNTATDPNVTVVAFKGTNTIYDAILDLNFSWYNVDQRIGNIHSGFMEALGLQKGTIGWP 240

Query: 241 KELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLE 300
           KEL K +H +AYY+LRQQLRDIAKSNDKA+FI TGHSLG ALA LFVTIL++H E D+L 
Sbjct: 241 KELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAILFVTILSYHNESDVLN 300

Query: 301 KLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHF 360
           KL+AVYTFGQPRVG+ QFV+FME+  SK + F YYRYVYSMD+VPRIPFD+  D+WYKHF
Sbjct: 301 KLQAVYTFGQPRVGNSQFVKFMES-TSKQYAFKYYRYVYSMDLVPRIPFDI-FDEWYKHF 360

Query: 361 GGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQ 420
           GGC+Y++C Y+G+F+E +PNKNY S  WLIPKYL+A WEL+RSL++P+I  +  YFE  +
Sbjct: 361 GGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELIRSLMMPLIKFSLYYFEGFK 420

Query: 421 IFYARVFGLFIPGASAHNVVNYIYSARWGKI-VCRDTQKKVIPADYLE 452
               R FGL IPGASAH  VNYIY AR GK+   +D QK +I  D LE
Sbjct: 421 TLLLRFFGLLIPGASAHLCVNYIYLARQGKMPPLKDIQKTLISEDGLE 464

BLAST of Moc09g00520 vs. ExPASy TrEMBL
Match: A0A6J1BSM4 (uncharacterized protein LOC111005368 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005368 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 2.4e-145
Identity = 264/451 (58.54%), Postives = 324/451 (71.84%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           MGS++ QF+ DYLILKPEEATLLDLFLF+LPFGF+ +RKLVDCP  KE SY S  +R II
Sbjct: 1   MGSEDHQFSDDYLILKPEEATLLDLFLFILPFGFRKIRKLVDCPAAKENSYRSIQSRLII 60

Query: 61  FFSILWQKILLAFLNQHKE-------------KPKTEDPPTVNCKDWSLSGRDKNIKL-D 120
           F SIL+QK++LA  N  K+             KP     P VNC+DW + G +KN+KL +
Sbjct: 61  FVSILFQKLVLAISNLAKKFKLMRGKLWAVSYKPYA---PKVNCRDWKIGG-EKNVKLPE 120

Query: 121 DPFQYYAILSVRASALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFM 180
           D F+YY+ L+V AS LA  D+SP P++V+TVV  CW M L+  Y   N F +   T  FM
Sbjct: 121 DNFKYYSALTVMASQLAYTDYSPQPNIVQTVVEGCWKMNLINTYKFRNGFIEKARTCAFM 180

Query: 181 FQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKE 240
           FQ T+TDPNV ++AF+GT+   D  VD++FSW  I  VG+IH GFM+ALGLQ+   WP E
Sbjct: 181 FQTTSTDPNVIILAFKGTSQCCDCAVDVNFSWCSIPGVGRIHMGFMQALGLQEAFHWPDE 240

Query: 241 LVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKL 300
           L KP  D+AYY LRQQLRDIAKSND ARFI TGHSLG ALA LFVTILA+H E D+LEKL
Sbjct: 241 LPKPNSDFAYYTLRQQLRDIAKSNDNARFIFTGHSLGGALAVLFVTILAYHNESDVLEKL 300

Query: 301 KAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQW--YKHF 360
           +AVYTFGQPRVGD +F QFMEN   K + F YYRYVYS D+V RIPF+  GD +  YKHF
Sbjct: 301 QAVYTFGQPRVGDPKFAQFMENTTKK-YDFKYYRYVYSFDLVSRIPFNC-GDFFCSYKHF 360

Query: 361 GGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQ 420
           GGC+Y+DC Y G+FLEE+PN NY    +LIP++L A WE +RSL++P++ C+ DYFE   
Sbjct: 361 GGCVYFDCCYNGKFLEEQPNNNYYWPIYLIPQFLAALWEFIRSLMIPLVKCSWDYFEGFG 420

Query: 421 IFYARVFGLFIPGASAHNVVNYIYSARWGKI 436
               RV GLFIP  SAH ++NYI S R+GKI
Sbjct: 421 NLKNRVLGLFIPATSAHMLLNYINSTRYGKI 445

BLAST of Moc09g00520 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 323.2 bits (827), Expect = 3.4e-88
Identity = 185/471 (39.28%), Postives = 265/471 (56.26%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESY-NSFGNRWI 60
           M    ++F + Y ++ P +A+ LDL L +      + R  +D PP+  + +  SF +RWI
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSAR-FIDSPPDTLKGFRRSFASRWI 60

Query: 61  IFFSILWQKILL------AFLNQ--------------------HKEKPKTEDPPTVNCKD 120
           +  +I  QK+L+      AF+ Q                    +    K   P   +   
Sbjct: 61  LALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATY 120

Query: 121 WSLSG-RDKNIKLDD-----PFQYYAILSVRASALATEDFSPHPSVVETVVNSCWNMKLL 180
            S  G  D+ I+LD+       +Y ++LS+ AS ++ E   P+   + +VV + W M L+
Sbjct: 121 TSFIGCSDRRIELDEKINVGSIEYKSMLSIMASKISYES-KPY---ITSVVKNTWKMDLV 180

Query: 181 GCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTT--DVYDMKVDLDFSWYEIANVG 240
           G YD +N FQ+   TQ F+F+ ++T+P++ VV+FRGT   +  D   DLD SWYE+ NVG
Sbjct: 181 GNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVG 240

Query: 241 KIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAA 300
           K+H GF  ALGLQK  GWPKE +   H YAYY +RQ LRD    N   ++I+TGHSLG A
Sbjct: 241 KVHAGFSRALGLQKD-GWPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGA 300

Query: 301 LATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSM 360
           LA LF  ILA H E +LL+KL+ +YTFGQPRVGD  F +FM+ V  K H   Y R+VY+ 
Sbjct: 301 LAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKK-HGIEYERFVYNN 360

Query: 361 DVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELL 420
           DVVPR+PFD +    YKH+G C  ++  Y G+  E+ PN NY ++ WLIP+ L   WE +
Sbjct: 361 DVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFI 420

Query: 421 RSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIV 437
           RS +L       +Y E   + + RV G+  PG S H   +Y+ S R G +V
Sbjct: 421 RSFILQ-FWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLV 463

BLAST of Moc09g00520 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.9e-86
Identity = 186/457 (40.70%), Postives = 262/457 (57.33%), Query Frame = 0

Query: 11  DYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEK-EESYNSFGNRWIIFFSILWQKI 70
           +Y +L P EAT+ DL   +      + RK +    E+ E+  + F  RWIIF SI+ QK+
Sbjct: 6   NYFVLDPREATVSDLIHLLFSSDLGD-RKFIHSSEERIEDDLSRFRRRWIIFVSIVIQKL 65

Query: 71  LLAF--------------------------LNQHKEKPKTEDPPTVNCKDWSLSGR-DKN 130
           ++ F                          +  +  K     P   +    SL+G  D+ 
Sbjct: 66  IILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLNGNLDRR 125

Query: 131 IKLDDPFQ-----YYAILSVRASALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQ 190
           ++L+   +     Y A+LS+ AS L+ E+     + V +V+++ W M LLG Y  WN +Q
Sbjct: 126 VELNPKVERGSKRYKAMLSIMASKLSYENI----NFVSSVLHNHWKMDLLGFYSCWNGYQ 185

Query: 191 DMGSTQVFMFQNTATDPNVTVVAFRGTT--DVYDMKVDLDFSWYEIANVGKIHRGFMEAL 250
              ST+V + ++T+TDPN+ +V+FRGT   D  D   DLD SWYE+ NVGKIH GFM+AL
Sbjct: 186 KQRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKAL 245

Query: 251 GLQKQLGWPKELVKPEHD-----YAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLF 310
           GLQK+ GWPKE+   E       YAYY +R+ L++I   N  ++FI+TGHSLG ALA LF
Sbjct: 246 GLQKE-GWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILF 305

Query: 311 VTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPR 370
             +L  H+E  +LE+L+ VYTFGQPRVGD +F  FM++ + K     Y RYVY  D+VPR
Sbjct: 306 TAVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKD-SLKKFDVKYERYVYCNDMVPR 365

Query: 371 IPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLL 428
           +PFD +    +KHFG CLYYD  Y G+  EEEPNKNY ++ W++PK + A WEL+RS ++
Sbjct: 366 LPFD-DKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVM 425

BLAST of Moc09g00520 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 290.4 bits (742), Expect = 2.5e-78
Identity = 187/485 (38.56%), Postives = 257/485 (52.99%), Query Frame = 0

Query: 11  DYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEK-EESYNSFGNRWIIFFSILWQKI 70
           +Y +L P EAT+ DL   +     +N RK VD   E  E     F  RWIIF SI+ QK+
Sbjct: 6   NYFVLDPREATVTDLMRLLFYSDLEN-RKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 65

Query: 71  LL----------------------------AFLNQHK------EKPKTEDPPTVNCKDWS 130
           L+                             FLN  K      EK            D  
Sbjct: 66  LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTSATFASINGNLDQK 125

Query: 131 LS-GRDKNIKLDDPFQYYAILSVRASALATEDFSPHPSVVETVVNSCWNMKLLGCYDLWN 190
           +  G  ++IK+ D  +Y  +LS+ AS LA E    +   + +V+   W M LLG Y   N
Sbjct: 126 VELGLGRSIKIGDE-RYKPLLSIMASKLAYE----NEHFIRSVLQDHWQMDLLGFYSCPN 185

Query: 191 DFQDMGSTQVFMFQNTATDPNVTVVAFRGTT--DVYDMKVDLDFSWYEIANVGKIHRGFM 250
           DF    ST+V + ++T  +PN+ VV+FRGT   +  D   DLD SW+ + NVGKIH GFM
Sbjct: 186 DFDQTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFM 245

Query: 251 EALGLQKQLGWPKELVKPE-----HDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALA 310
           +ALGL K+ GW +E+   +        AYY + +QL+++ + N  ++FI++GHSLG ALA
Sbjct: 246 KALGLPKE-GWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALA 305

Query: 311 TLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDV 370
            LF  +L  H+E ++LE+L+ VYTFGQPRVGD  F  +M++   K     Y RYVY  D+
Sbjct: 306 ILFTAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKD-KLKEFDVKYKRYVYCNDM 365

Query: 371 VPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRS 430
           VPR+PFD +    +KHFGGCLY D  Y G+  EEEPNKNY +I W+IPK + A WEL+RS
Sbjct: 366 VPRLPFD-DKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRS 425

Query: 431 LLLPVIMC--NPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDTQKKVI 451
               +I C    +Y E   +   R+  L IPG  AH    Y+  A  G    +    + +
Sbjct: 426 F---IISCWQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGNFPPQVPDSQNL 478

BLAST of Moc09g00520 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 270.0 bits (689), Expect = 3.4e-72
Identity = 156/392 (39.80%), Postives = 223/392 (56.89%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESY-NSFGNRWI 60
           M    ++F + Y ++ P +A+ LDL L +      + R  +D PP+  + +  SF +RWI
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSAR-FIDSPPDTLKGFRRSFASRWI 60

Query: 61  IFFSILWQKILL------AFLNQ--------------------HKEKPKTEDPPTVNCKD 120
           +  +I  QK+L+      AF+ Q                    +    K   P   +   
Sbjct: 61  LALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATY 120

Query: 121 WSLSG-RDKNIKLDD-----PFQYYAILSVRASALATEDFSPHPSVVETVVNSCWNMKLL 180
            S  G  D+ I+LD+       +Y ++LS+ AS ++ E   P+   + +VV + W M L+
Sbjct: 121 TSFIGCSDRRIELDEKINVGSIEYKSMLSIMASKISYES-KPY---ITSVVKNTWKMDLV 180

Query: 181 GCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTT--DVYDMKVDLDFSWYEIANVG 240
           G YD +N FQ+   TQ F+F+ ++T+P++ VV+FRGT   +  D   DLD SWYE+ NVG
Sbjct: 181 GNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVG 240

Query: 241 KIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAA 300
           K+H GF  ALGLQK  GWPKE +   H YAYY +RQ LRD    N   ++I+TGHSLG A
Sbjct: 241 KVHAGFSRALGLQKD-GWPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGA 300

Query: 301 LATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSM 358
           LA LF  ILA H E +LL+KL+ +YTFGQPRVGD  F +FM+ V  K H   Y R+VY+ 
Sbjct: 301 LAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKK-HGIEYERFVYNN 360

BLAST of Moc09g00520 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 2.2e-71
Identity = 163/471 (34.61%), Postives = 242/471 (51.38%), Query Frame = 0

Query: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60
           M S +      YLIL+PEE    +L   +     +  R +       E   +SF +RW+I
Sbjct: 2   MNSDDDDEPRGYLILRPEELRPWELVRLLFSGDIEKPRSV----DSSETEEHSFRHRWLI 61

Query: 61  FFSILWQKIL-----------------LAFLNQHK-----EKPKTEDPPTVNCKDWSLSG 120
           F S++  K+L                 L FL+ +       + +   P   +    S  G
Sbjct: 62  FVSLVLLKLLRFFSKLLALVGSALEFSLNFLSNNSFSGLFLRGEVVMPQRTSENYQSFIG 121

Query: 121 R-------DKNIKLDDPFQYYAILSVRASALATEDFSPHPSVVETVVNSCWNMKLLGCYD 180
                   D  +  +D  +YYA LS+ AS +A E    + + ++ VV + WNMK LG  D
Sbjct: 122 HLDTRVSLDMTLNREDGEKYYAALSIMASKIAYE----NAARIKHVVENHWNMKYLGLVD 181

Query: 181 LWNDFQDMGSTQVFMFQNTAT------DPNVTVVAFRGTT--DVYDMKVDLDFSWYEIAN 240
            WN++Q+  +TQ F+     T           VVAFRGT   +  D   D D +W+E+ N
Sbjct: 182 YWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPN 241

Query: 241 VGKIHRGFMEALGLQKQLGWPKE-LVKPEH--DYAYYFLRQQLRDIAKSNDKARFIITGH 300
           +G IH GFM+ALGLQ    WPKE L  P+     AYY +R  L+ +   N   +F++TGH
Sbjct: 242 IGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGH 301

Query: 301 SLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYR 360
           SLG ALA LF  +L  H E +LLE+++ VYT+GQPRVGD +F +FME    K +   YYR
Sbjct: 302 SLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEK-YNIKYYR 361

Query: 361 YVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYA 420
           +VY+ D+VPR+P+D + D  +KHFG C+YYD NY  + + E+ ++N+  +  +I     A
Sbjct: 362 FVYNNDIVPRLPYD-DKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSA 421

Query: 421 WWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSAR 432
             E +RS  + V     +Y E   +   R  G+ +PG S H   +Y+ + R
Sbjct: 422 ILEFIRSFTI-VAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATR 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152547.15.9e-276100.00uncharacterized protein LOC111020243 [Momordica charantia][more]
XP_022139747.13.0e-17164.33uncharacterized protein LOC111010582 [Momordica charantia][more]
XP_022135571.13.7e-16163.09uncharacterized protein LOC111007483 isoform X2 [Momordica charantia][more]
XP_022135562.17.0e-16063.03uncharacterized protein LOC111007483 isoform X1 [Momordica charantia][more]
XP_022132571.14.9e-14558.54uncharacterized protein LOC111005368 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
F4JFU87.5e-6434.20Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1[more]
A0A1S3ZP853.1e-6237.79Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1[more]
Q5VKJ72.0e-5638.15Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1[more]
P195151.3e-0730.13Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2[more]
O599521.3e-0725.21Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DGJ72.9e-276100.00uncharacterized protein LOC111020243 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1CEU21.5e-17164.33uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1C1481.8e-16163.09uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1C3213.4e-16063.03uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1BSM42.4e-14558.54uncharacterized protein LOC111005368 isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT1G45201.13.4e-8839.28triacylglycerol lipase-like 1 [more]
AT5G42930.11.9e-8640.70alpha/beta-Hydrolases superfamily protein [more]
AT1G56630.12.5e-7838.56alpha/beta-Hydrolases superfamily protein [more]
AT1G45201.23.4e-7239.80triacylglycerol lipase-like 1 [more]
AT5G67050.12.2e-7134.61alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 178..334
e-value: 1.0E-35
score: 122.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 109..370
e-value: 4.7E-44
score: 152.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 153..364
IPR044819Triacylglycerol lipase OBL1-likePANTHERPTHR46086ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..73
IPR044819Triacylglycerol lipase OBL1-likePANTHERPTHR46086ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 88..438
NoneNo IPR availablePANTHERPTHR46086:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..73
NoneNo IPR availablePANTHERPTHR46086:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 88..438
NoneNo IPR availableCDDcd00519Lipase_3coord: 150..357
e-value: 1.49752E-40
score: 143.001

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g00520.1Moc09g00520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0004806 triglyceride lipase activity