Moc08g40150 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g40150
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	(R)-mandelonitrile lyase 1-like
Location	chr8: 30767912 .. 30769356 (+)
RNA-Seq Expression	Moc08g40150
Synteny	Moc08g40150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTTTTCTCTTGTTCATCATTTTCTTTATCGGACTCTTGATCTCTATCAACACCCAACCCAATCAAGGTAAAATCCCTCTCTCCAACTCTGGATTCATCTTATACAAAAATTAAGGAACCATTTCGTCTTTTATTTTTCTTTTTTTGGAAAAACTACTCCATTGAAGTATGTGAGAAACATAACAAAAGAAAGTTTCTTATTAACAAAAAAAGTATGTGAGAAACATATCGCACCTTTTGTTCTTTAACTTATTTTTCATATCTTTTGTAAACACACTTTCTTATTAGCAAGATTTCAGAAGCAAAAACAAAGCTTTTAAAAGCTATACCTAATTGTTGTCATTGCCAAATTCGCTCACTCTTTCCTCTACCTATTTCGTGAAAGGGACTACCTACTTCGCGTGACAGTGAAAGGGGAGAGCCAAACCGGGAAAATACTTGACCCCTCAGCAATGAGGAGGACATTGAATTTGGGGTGAAAAATTAAGTAGTGTTAGGTTTAAAAATTAAAGTTTGGGCCACTTTTAATGTTGAACTCAAACGTTGATCTATAATGTCGAGTCTAACCCGACATTGAAATTTAATTTATTTTTTTATTTTTATTTTTTATTTTTTACATTGTCCGTCATTATTTCTTCCATATTTATTTTTTATCAGTAAACTCACATTTGTTGAAGTTAGATTTCAAAACGGTTTTAAAAGAGAAAGATATAACAAACAAACAACTAAGAATAAAATAGTTATCCAATCGTGTCCTAAATTTTCAAATTTTTTTCTCTAATTTGTTCCATCTCTTTAAATTTTTAGAATTGATAAACTACATATAACAAAAGTTAGAACATAGTATGATTTTGTACTCCTTCCTCTAAAAATTTCAAACTCCATTGAACACAGTCTCATTATTTTAAACTTTTTGTTCAGATGCTAGCTATATGAAGTTTGTGTACAATGCCAGTAGTCTACCAGAAAAAGAAGAATATGACTACATAATAATAGGGGGAGGGACTGCAGGCTGCCCATTAGCCGCAACTTTGTCAAGAAATTTCTCTGTCCTCCTCGAAAGAGGCAGTGTCCCCAACAAATATCTCTCTGTACTGAGCGAACAAGGTCTACAAAACGTTTTCACTGTGGAGGATGACGGCAAAAATCCCTTCCAGCGGTTCATCTCTGAGGAGGGTGTAGAGAACCTAAGAGGGCGGATCCTTGGCGGTAGTAGCATGATCAACGGCGGCGTCTACTCGAGAGCAAATCCAGAGTTCTTCAGAACTCAGGGCGTGGAATGGGACATGGAAATGGTGGAGAAGGCGTACCAATGGGTGGAACAGGCTTTGGTTTCGCGCCCGAGTTTAGTTCCTTGGCAATCTGCTTTTAAAAGTGCTTTGGTGGAAAGTGGTGTTGTCCATGATAATGTATTCGATTTGAGAGATTGTGGGGACTAA

mRNA sequence

ATGGCTTCTTTTCTCTTGTTCATCATTTTCTTTATCGGACTCTTGATCTCTATCAACACCCAACCCAATCAAGATGCTAGCTATATGAAGTTTGTGTACAATGCCAGTAGTCTACCAGAAAAAGAAGAATATGACTACATAATAATAGGGGGAGGGACTGCAGGCTGCCCATTAGCCGCAACTTTGTCAAGAAATTTCTCTGTCCTCCTCGAAAGAGGCAGTGTCCCCAACAAATATCTCTCTGTACTGAGCGAACAAGGTCTACAAAACGTTTTCACTGTGGAGGATGACGGCAAAAATCCCTTCCAGCGGTTCATCTCTGAGGAGGGTGTAGAGAACCTAAGAGGGCGGATCCTTGGCGGTAGTAGCATGATCAACGGCGGCGTCTACTCGAGAGCAAATCCAGAGTTCTTCAGAACTCAGGGCGTGGAATGGGACATGGAAATGGTGGAGAAGGCGTACCAATGGGTGGAACAGGCTTTGGTTTCGCGCCCGAGTTTAGTTCCTTGGCAATCTGCTTTTAAAAGTGCTTTGGTGGAAAGTGGTGTTGTCCATGATAATGTATTCGATTTGAGAGATTGTGGGGACTAA

Coding sequence (CDS)

ATGGCTTCTTTTCTCTTGTTCATCATTTTCTTTATCGGACTCTTGATCTCTATCAACACCCAACCCAATCAAGATGCTAGCTATATGAAGTTTGTGTACAATGCCAGTAGTCTACCAGAAAAAGAAGAATATGACTACATAATAATAGGGGGAGGGACTGCAGGCTGCCCATTAGCCGCAACTTTGTCAAGAAATTTCTCTGTCCTCCTCGAAAGAGGCAGTGTCCCCAACAAATATCTCTCTGTACTGAGCGAACAAGGTCTACAAAACGTTTTCACTGTGGAGGATGACGGCAAAAATCCCTTCCAGCGGTTCATCTCTGAGGAGGGTGTAGAGAACCTAAGAGGGCGGATCCTTGGCGGTAGTAGCATGATCAACGGCGGCGTCTACTCGAGAGCAAATCCAGAGTTCTTCAGAACTCAGGGCGTGGAATGGGACATGGAAATGGTGGAGAAGGCGTACCAATGGGTGGAACAGGCTTTGGTTTCGCGCCCGAGTTTAGTTCCTTGGCAATCTGCTTTTAAAAGTGCTTTGGTGGAAAGTGGTGTTGTCCATGATAATGTATTCGATTTGAGAGATTGTGGGGACTAA

Protein sequence

MASFLLFIIFFIGLLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLSRNFSVLLERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGSSMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESGVVHDNVFDLRDCGD

Homology

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BLAST of Moc08g40150 vs. NCBI nr
Match: XP_022929724.1 ((R)-mandelonitrile lyase 1-like [Cucurbita moschata])

HSP 1 Score: 275.8 bits (704), Expect = 2.9e-70
Identity = 140/201 (69.65%), Postives = 166/201 (82.59%), Query Frame = 0


Query: 1   MASFLLFIIFFIG-----LLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAG 60
           M +FLL ++ F+      LLIS NT+PNQD SYMKFV++ +  PEKEEYDYII+GGGTAG
Sbjct: 6   MPTFLLLVLLFLSHSQLLLLISTNTRPNQDISYMKFVHDGNDFPEKEEYDYIIVGGGTAG 65

Query: 61  CPLAATLSRNFSVLL-ERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENL 120
           CPLAATLS+NFS LL ERGS PNKY SVL EQ L NVFTVEDDGKNP+ RF S+EGV+NL
Sbjct: 66  CPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPYNRFTSKEGVQNL 125

Query: 121 RGRILGGSSMINGGVYSRANPEFFRTQ-GVE-WDMEMVEKAYQWVEQALVSRPSLVPWQS 180
           RGR+LGG+SMINGGVYS+ N +FF+TQ GV+ +DMEMVEKAY+WVE+ALV RPSL  WQ+
Sbjct: 126 RGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRPSLNAWQN 185

Query: 181 AFKSALVESGVVHDNVFDLRD 194
           AF+  LVE G+V DN F+LRD
Sbjct: 186 AFRRILVEGGIVPDNGFELRD 206

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BLAST of Moc08g40150 vs. NCBI nr
Match: XP_023546914.1 ((R)-mandelonitrile lyase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 273.9 bits (699), Expect = 1.1e-69
Identity = 141/201 (70.15%), Postives = 164/201 (81.59%), Query Frame = 0


Query: 1   MASFLLFIIFFIG-----LLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAG 60
           M +FLL I+ F+      L IS NTQPNQD SYMKFV + +  PEKEEYDYII+GGGTAG
Sbjct: 6   MPTFLLLILLFLSHSQLLLSISTNTQPNQDISYMKFVRDGNDFPEKEEYDYIIVGGGTAG 65

Query: 61  CPLAATLSRNFSVLL-ERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENL 120
           CPLAATLS+NFS LL ERGS PNKY SVL EQ L NVFTVEDDGKNP+ RF S+EGV+NL
Sbjct: 66  CPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPYNRFTSKEGVQNL 125

Query: 121 RGRILGGSSMINGGVYSRANPEFFRTQ-GVE-WDMEMVEKAYQWVEQALVSRPSLVPWQS 180
           RGR+LGG+SMINGGVYS+ N +FF+TQ GV+ +DMEMVEKAY+WVE+ALV RPSL  WQ+
Sbjct: 126 RGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRPSLNAWQN 185

Query: 181 AFKSALVESGVVHDNVFDLRD 194
           AF+  LVE G+V DN F+LRD
Sbjct: 186 AFRRILVEGGIVPDNGFELRD 206

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BLAST of Moc08g40150 vs. NCBI nr
Match: XP_022997400.1 ((R)-mandelonitrile lyase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 273.9 bits (699), Expect = 1.1e-69
Identity = 140/201 (69.65%), Postives = 164/201 (81.59%), Query Frame = 0


Query: 1   MASFLLFIIFFIG-----LLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAG 60
           M +FLL I+  +      LLIS NTQPN+D SYMKFV + +  PEKEEYDYII+GGGTAG
Sbjct: 6   MPTFLLLILLLLSHSQLRLLISTNTQPNEDISYMKFVRDGNDFPEKEEYDYIIVGGGTAG 65

Query: 61  CPLAATLSRNFSVLL-ERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENL 120
           CPLAATLS+NFS LL ERGS PNKY SVL EQ L NVFTVEDDGKNPF RF+S+EGV+NL
Sbjct: 66  CPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPFNRFVSKEGVQNL 125

Query: 121 RGRILGGSSMINGGVYSRANPEFFRTQ-GVE-WDMEMVEKAYQWVEQALVSRPSLVPWQS 180
           RGR+LGG+SMINGGVYS+ N +FF+TQ GV+ +DMEMVEKAY+WVE+ALV RP L  WQ+
Sbjct: 126 RGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRPRLNAWQN 185

Query: 181 AFKSALVESGVVHDNVFDLRD 194
           AF+  LVE G+V DN F+LRD
Sbjct: 186 AFRRVLVEGGIVPDNGFELRD 206

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BLAST of Moc08g40150 vs. NCBI nr
Match: XP_031736478.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61117.1 hypothetical protein Csa_021176 [Cucumis sativus])

HSP 1 Score: 271.6 bits (693), Expect = 5.5e-69
Identity = 135/190 (71.05%), Postives = 154/190 (81.05%), Query Frame = 0


Query: 4   FLLFIIFFIGLLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLS 63
           F L  IF +GL+ S +T PNQD SYMKFVY AS L E EEYDYIIIGGGTAGCPLAATLS
Sbjct: 12  FTLISIFQLGLIAS-HTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGTAGCPLAATLS 71

Query: 64  RNFSV-LLERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGS 123
             FSV LLERG+ PNKY SVL+EQGL N F  EDDG+NPFQ FISE+GVENLRGR+LGG 
Sbjct: 72  SKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLGGG 131

Query: 124 SMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESG 183
           SMIN G YSR + +FF T GV+WD E+VEKAY+WVE+ +VS+PSL PWQ+AF+SAL+E G
Sbjct: 132 SMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGG 191

Query: 184 VVHDNVFDLR 193
           V HD  FDLR
Sbjct: 192 VGHDKGFDLR 200

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BLAST of Moc08g40150 vs. NCBI nr
Match: XP_008451922.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10909.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 271.2 bits (692), Expect = 7.2e-69
Identity = 135/190 (71.05%), Postives = 155/190 (81.58%), Query Frame = 0


Query: 4   FLLFIIFFIGLLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLS 63
           F L  IF +G +IS +  PNQD SYMKFV+ AS L E EEYDYIIIGGGTAGCPLAATLS
Sbjct: 8   FTLISIFQLG-VISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAATLS 67

Query: 64  RNFSV-LLERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGS 123
             FSV LLERG+ PNKY SVL+EQGL N F  EDDG+NPFQ FISE+GVENLRGR+LGG 
Sbjct: 68  SKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLGGG 127

Query: 124 SMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESG 183
           SMIN G YSR + EFF T GV+WDME+VEKAY+WVE+ +VS+PSL PWQ+AF+SAL+E G
Sbjct: 128 SMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGG 187

Query: 184 VVHDNVFDLR 193
           V HDN FDL+
Sbjct: 188 VGHDNGFDLK 196

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BLAST of Moc08g40150 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 7.7e-50
Identity = 92/174 (52.87%), Postives = 124/174 (71.26%), Query Frame = 0


Query: 19  NTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLSRNFSVL-LERGSVPN 78
           NT    D  Y+KFVYNA  L  +  YDYII+GGGT+GCPLAATLS N+SVL LERG++  
Sbjct: 30  NTSSEHDFGYLKFVYNAVDLELEGSYDYIIVGGGTSGCPLAATLSANYSVLVLERGTIAT 89

Query: 79  KYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGSSMINGGVYSRANPEF 138
           +Y + L+  G       +DDGK P +RF+SE+G++N+R RILGG+++IN GVY+RAN  F
Sbjct: 90  EYPNTLTVDGFAYNLQQQDDGKTPVERFVSEDGIDNVRSRILGGTTIINAGVYARANESF 149

Query: 139 FRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESGVVHDNVFDL 192
           +   GVEWD+++V +AY+WVE A+V +PS   WQS   +A +E+GV  DN F L
Sbjct: 150 YNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQSWQSITGTAFLEAGVHPDNGFGL 203

[Close]

BLAST of Moc08g40150 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.0e-49
Identity = 96/197 (48.73%), Postives = 135/197 (68.53%), Query Frame = 0


Query: 1   MASFLLFIIFFIGLL-----ISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAG 60
           M++ LL +  F+ LL      S+ T  N D SY++F Y+A+ L  +  YDY+I+GGGT+G
Sbjct: 6   MSAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSG 65

Query: 61  CPLAATLSRNFSVL-LERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENL 120
           CPLAATLS  + VL LERGS+P  Y +VL+  G       EDDGK P +RF+SE+G++N+
Sbjct: 66  CPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 121 RGRILGGSSMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAF 180
           RGR+LGG+SMIN GVY+RAN   +   GV+WDM++V K Y+WVE  +V +P+  PWQS  
Sbjct: 126 RGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVT 185

Query: 181 KSALVESGVVHDNVFDL 192
            +A +E+GV  ++ F L
Sbjct: 186 GTAFLEAGVDPNHGFSL 202

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BLAST of Moc08g40150 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 8.5e-49
Identity = 89/176 (50.57%), Postives = 123/176 (69.89%), Query Frame = 0


Query: 17  SINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLSRNFSVL-LERGSV 76
           S+    + D SY+ FVY+A+    +  YDYII+GGGTAGCPLAATLS N+SVL LERGS+
Sbjct: 27  SLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSL 86

Query: 77  PNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGSSMINGGVYSRANP 136
           P +Y ++L   G       EDDGK P +RF+SE+G++N+RGR+LGG+SMIN GVY RAN 
Sbjct: 87  PTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANT 146

Query: 137 EFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESGVVHDNVFDL 192
            FF   G+EWDM++V + Y+WVE  +V  P    WQ+   +A +E+G++ +N F +
Sbjct: 147 SFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSV 202

[Close]

BLAST of Moc08g40150 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 9.4e-48
Identity = 88/168 (52.38%), Postives = 120/168 (71.43%), Query Frame = 0


Query: 25  DASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLSRNFSVL-LERGSVPNKYLSVL 84
           D SY++FVY+A+    +  YDYII+GGGTAGCPLAATLS N+SVL LERG++P +Y ++L
Sbjct: 36  DFSYLEFVYDANDTELEGTYDYIIVGGGTAGCPLAATLSANYSVLVLERGTLPTEYPNLL 95

Query: 85  SEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGSSMINGGVYSRANPEFFRTQGV 144
           +  G       EDDG+ P +RF+S +G++N+RGR+LGG+SMIN GVY RAN  FF   G+
Sbjct: 96  TSDGFIYNLQQEDDGQTPVERFVSGDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGI 155

Query: 145 EWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESGVVHDNVFDL 192
           EWDM++V K Y WVE  +V +P    WQ+   +A +E G++ DN F L
Sbjct: 156 EWDMDLVNKTYDWVEDTIVFKPDFQFWQNLTGTAFLEVGILPDNGFSL 203

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BLAST of Moc08g40150 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 2.7e-47
Identity = 91/197 (46.19%), Postives = 133/197 (67.51%), Query Frame = 0


Query: 1   MASFLLFIIFFI-----GLLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAG 60
           M++ LL +  F+       + S+ T  + D SY+ F Y+A+ L  +  YDY+I+GGGT+G
Sbjct: 6   MSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSG 65

Query: 61  CPLAATLSRNFSVL-LERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENL 120
           CPLAATLS  + VL LERGS+P  Y +VL+  G       EDDGK P +RF+SE+G++N+
Sbjct: 66  CPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 121 RGRILGGSSMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAF 180
           RGR+LGG+S+IN GVY+RAN   +   GV+WDM++V + Y+WVE  +V +P+   WQS  
Sbjct: 126 RGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVT 185

Query: 181 KSALVESGVVHDNVFDL 192
           K+A +E+GV  ++ F L
Sbjct: 186 KTAFLEAGVHPNHGFSL 202

[Close]

BLAST of Moc08g40150 vs. ExPASy TrEMBL
Match: A0A6J1EPL5 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436223 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.4e-70
Identity = 140/201 (69.65%), Postives = 166/201 (82.59%), Query Frame = 0


Query: 1   MASFLLFIIFFIG-----LLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAG 60
           M +FLL ++ F+      LLIS NT+PNQD SYMKFV++ +  PEKEEYDYII+GGGTAG
Sbjct: 6   MPTFLLLVLLFLSHSQLLLLISTNTRPNQDISYMKFVHDGNDFPEKEEYDYIIVGGGTAG 65

Query: 61  CPLAATLSRNFSVLL-ERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENL 120
           CPLAATLS+NFS LL ERGS PNKY SVL EQ L NVFTVEDDGKNP+ RF S+EGV+NL
Sbjct: 66  CPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPYNRFTSKEGVQNL 125

Query: 121 RGRILGGSSMINGGVYSRANPEFFRTQ-GVE-WDMEMVEKAYQWVEQALVSRPSLVPWQS 180
           RGR+LGG+SMINGGVYS+ N +FF+TQ GV+ +DMEMVEKAY+WVE+ALV RPSL  WQ+
Sbjct: 126 RGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRPSLNAWQN 185

Query: 181 AFKSALVESGVVHDNVFDLRD 194
           AF+  LVE G+V DN F+LRD
Sbjct: 186 AFRRILVEGGIVPDNGFELRD 206

[Close]

BLAST of Moc08g40150 vs. ExPASy TrEMBL
Match: A0A6J1K9H9 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492330 PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 5.3e-70
Identity = 140/201 (69.65%), Postives = 164/201 (81.59%), Query Frame = 0


Query: 1   MASFLLFIIFFIG-----LLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAG 60
           M +FLL I+  +      LLIS NTQPN+D SYMKFV + +  PEKEEYDYII+GGGTAG
Sbjct: 6   MPTFLLLILLLLSHSQLRLLISTNTQPNEDISYMKFVRDGNDFPEKEEYDYIIVGGGTAG 65

Query: 61  CPLAATLSRNFSVLL-ERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENL 120
           CPLAATLS+NFS LL ERGS PNKY SVL EQ L NVFTVEDDGKNPF RF+S+EGV+NL
Sbjct: 66  CPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPFNRFVSKEGVQNL 125

Query: 121 RGRILGGSSMINGGVYSRANPEFFRTQ-GVE-WDMEMVEKAYQWVEQALVSRPSLVPWQS 180
           RGR+LGG+SMINGGVYS+ N +FF+TQ GV+ +DMEMVEKAY+WVE+ALV RP L  WQ+
Sbjct: 126 RGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRPRLNAWQN 185

Query: 181 AFKSALVESGVVHDNVFDLRD 194
           AF+  LVE G+V DN F+LRD
Sbjct: 186 AFRRVLVEGGIVPDNGFELRD 206

[Close]

BLAST of Moc08g40150 vs. ExPASy TrEMBL
Match: A0A0A0LK79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055050 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 2.7e-69
Identity = 135/190 (71.05%), Postives = 154/190 (81.05%), Query Frame = 0


Query: 4   FLLFIIFFIGLLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLS 63
           F L  IF +GL+ S +T PNQD SYMKFVY AS L E EEYDYIIIGGGTAGCPLAATLS
Sbjct: 12  FTLISIFQLGLIAS-HTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGTAGCPLAATLS 71

Query: 64  RNFSV-LLERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGS 123
             FSV LLERG+ PNKY SVL+EQGL N F  EDDG+NPFQ FISE+GVENLRGR+LGG 
Sbjct: 72  SKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLGGG 131

Query: 124 SMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESG 183
           SMIN G YSR + +FF T GV+WD E+VEKAY+WVE+ +VS+PSL PWQ+AF+SAL+E G
Sbjct: 132 SMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGG 191

Query: 184 VVHDNVFDLR 193
           V HD  FDLR
Sbjct: 192 VGHDKGFDLR 200

[Close]

BLAST of Moc08g40150 vs. ExPASy TrEMBL
Match: A0A5D3CL00 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00890 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 3.5e-69
Identity = 135/190 (71.05%), Postives = 155/190 (81.58%), Query Frame = 0


Query: 4   FLLFIIFFIGLLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLS 63
           F L  IF +G +IS +  PNQD SYMKFV+ AS L E EEYDYIIIGGGTAGCPLAATLS
Sbjct: 8   FTLISIFQLG-VISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAATLS 67

Query: 64  RNFSV-LLERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGS 123
             FSV LLERG+ PNKY SVL+EQGL N F  EDDG+NPFQ FISE+GVENLRGR+LGG 
Sbjct: 68  SKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLGGG 127

Query: 124 SMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESG 183
           SMIN G YSR + EFF T GV+WDME+VEKAY+WVE+ +VS+PSL PWQ+AF+SAL+E G
Sbjct: 128 SMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGG 187

Query: 184 VVHDNVFDLR 193
           V HDN FDL+
Sbjct: 188 VGHDNGFDLK 196

[Close]

BLAST of Moc08g40150 vs. ExPASy TrEMBL
Match: A0A1S3BSP4 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 3.5e-69
Identity = 135/190 (71.05%), Postives = 155/190 (81.58%), Query Frame = 0


Query: 4   FLLFIIFFIGLLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLS 63
           F L  IF +G +IS +  PNQD SYMKFV+ AS L E EEYDYIIIGGGTAGCPLAATLS
Sbjct: 8   FTLISIFQLG-VISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAATLS 67

Query: 64  RNFSV-LLERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGS 123
             FSV LLERG+ PNKY SVL+EQGL N F  EDDG+NPFQ FISE+GVENLRGR+LGG 
Sbjct: 68  SKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLGGG 127

Query: 124 SMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESG 183
           SMIN G YSR + EFF T GV+WDME+VEKAY+WVE+ +VS+PSL PWQ+AF+SAL+E G
Sbjct: 128 SMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLEGG 187

Query: 184 VVHDNVFDLR 193
           V HDN FDL+
Sbjct: 188 VGHDNGFDLK 196

[Close]

BLAST of Moc08g40150 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 181.8 bits (460), Expect = 5.3e-46
Identity = 92/189 (48.68%), Postives = 126/189 (66.67%), Query Frame = 0


Query: 6   LFIIFFIGLLISINTQP--NQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLS 65
           L ++  +G++   N +P  N+   +M+F+ NA+    ++ YDYII+GGGTAGCPLAATLS
Sbjct: 14  LVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLS 73

Query: 66  RNFSV-LLERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILGGS 125
           ++F V LLERG VP    +V+S  G     T  ++  +P Q FISEEGV N RGR+LGGS
Sbjct: 74  QSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGS 133

Query: 126 SMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESG 185
           S IN G YSRA+ +FF   G+ WD+  V ++Y+WVE+A+V RP L  WQ+A + AL+E G
Sbjct: 134 SAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVG 193

Query: 186 VVHDNVFDL 192
           V   N F L
Sbjct: 194 VHPFNGFTL 202

[Close]

BLAST of Moc08g40150 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 145.2 bits (365), Expect = 5.5e-35
Identity = 86/187 (45.99%), Postives = 116/187 (62.03%), Query Frame = 0


Query: 5   LLFIIFFIGLLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLSR 64
           L++I  F   L+ + T          F+ +A+  P+   +DYIIIGGGTAGC LAATLS+
Sbjct: 43  LVYISIFFFFLVKLET---------TFMKDATLAPKNASFDYIIIGGGTAGCALAATLSQ 102

Query: 65  NFSVL-LERGSVPNKYLSVLSEQGLQNVFTVEDDGKNPF-QRFISEEGVENLRGRILGGS 124
           N SVL LERG  P  Y +  +     +V T+ ++  N + Q FISE+GV N R R+LGG 
Sbjct: 103 NASVLVLERGGSP--YENPTATDMGNSVNTLLNNTPNSWSQLFISEDGVYNTRPRVLGGG 162

Query: 125 SMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVESG 184
           S+INGG YSRA  ++   +  EW+ME VE AY+WVE+ LV  P ++ WQ AFK  L+E+G
Sbjct: 163 SVINGGFYSRAGNDY--VEEAEWEMEEVEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAG 216

Query: 185 VVHDNVF 190
              DN F
Sbjct: 223 ESPDNGF 216

[Close]

BLAST of Moc08g40150 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.5e-32
Identity = 81/189 (42.86%), Postives = 111/189 (58.73%), Query Frame = 0


Query: 4   FLLFIIFFIGLLISINTQPNQDASYMKFVYNASSLPEKEEYDYIIIGGGTAGCPLAATLS 63
           F+   +F    + S +  PN       F+ +A+  P    YDYIIIGGGTAGCPLAATLS
Sbjct: 11  FVALSLFLHSPICSSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLS 70

Query: 64  RNFSV-LLERGSVP--NKYLSVLSEQGLQNVFTVEDDGKNPFQRFISEEGVENLRGRILG 123
           +N SV LLERG  P  N  ++ LS  G       E    +P QRF+SE+GV N R R+LG
Sbjct: 71  QNASVLLLERGDSPYNNPNITRLSAFGAALSDLSE---SSPSQRFVSEDGVINARARVLG 130

Query: 124 GSSMINGGVYSRANPEFFRTQGVEWDMEMVEKAYQWVEQALVSRPSLVPWQSAFKSALVE 183
           G S +N G Y+RA  ++ R  G  WD  +  ++YQWVE  +  +P +  WQ+A +  L+E
Sbjct: 131 GGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLE 189

Query: 184 SGVVHDNVF 190
           +G+V +N F
Sbjct: 191 AGIVPNNGF 189

[Close]

BLAST of Moc08g40150 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 135.6 bits (340), Expect = 4.4e-32
Identity = 75/157 (47.77%), Postives = 99/157 (63.06%), Query Frame = 0


Query: 36  SSLPEKEEYDYIIIGGGTAGCPLAATLSRNFSVL-LERGSVP--NKYLSVLSEQGLQNVF 95
           SS  +   YDYI+IGGGTAGCPLAATLS+NFSVL LERG VP  N  +S L      ++ 
Sbjct: 55  SSNGQDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIG 114

Query: 96  TVEDDGKNPFQRFISEEGVENLRGRILGGSSMINGGVYSRANPEFFRTQGVEWDMEMVEK 155
             +    +  Q F+S +GV N R R+LGG S IN G YSRA+  F +  G  WD ++V++
Sbjct: 115 LADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKE 174

Query: 156 AYQWVEQALVSRPSLVPWQSAFKSALVESGVVHDNVF 190
           +Y WVE+ +V +P L  WQ A + +L+E GV   N F
Sbjct: 175 SYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGF 206

[Close]

BLAST of Moc08g40150 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 135.6 bits (340), Expect = 4.4e-32
Identity = 75/157 (47.77%), Postives = 99/157 (63.06%), Query Frame = 0


Query: 36  SSLPEKEEYDYIIIGGGTAGCPLAATLSRNFSVL-LERGSVP--NKYLSVLSEQGLQNVF 95
           SS  +   YDYI+IGGGTAGCPLAATLS+NFSVL LERG VP  N  +S L      ++ 
Sbjct: 55  SSNGQDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIG 114

Query: 96  TVEDDGKNPFQRFISEEGVENLRGRILGGSSMINGGVYSRANPEFFRTQGVEWDMEMVEK 155
             +    +  Q F+S +GV N R R+LGG S IN G YSRA+  F +  G  WD ++V++
Sbjct: 115 LADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKE 174

Query: 156 AYQWVEQALVSRPSLVPWQSAFKSALVESGVVHDNVF 190
           +Y WVE+ +V +P L  WQ A + +L+E GV   N F
Sbjct: 175 SYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGF 206

The following BLAST results are available for this feature:

BLAST of Moc08g40150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022929724.1	2.9e-70	69.65	(R)-mandelonitrile lyase 1-like [Cucurbita moschata]	[more]
XP_023546914.1	1.1e-69	70.15	(R)-mandelonitrile lyase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997400.1	1.1e-69	69.65	(R)-mandelonitrile lyase 1-like isoform X1 [Cucurbita maxima]	[more]
XP_031736478.1	5.5e-69	71.05	(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61117.1 hypothetical prote...	[more]
XP_008451922.1	7.2e-69	71.05	PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mand...	[more]

BLAST of Moc08g40150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82784	7.7e-50	52.87	(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1	[more]
P52706	1.0e-49	48.73	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
P52707	8.5e-49	50.57	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
O50048	9.4e-48	52.38	(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1	[more]
Q945K2	2.7e-47	46.19	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]

BLAST of Moc08g40150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EPL5	1.4e-70	69.65	(R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436223 PE...	[more]
A0A6J1K9H9	5.3e-70	69.65	(R)-mandelonitrile lyase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A0A0LK79	2.7e-69	71.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055050 PE=3 SV=1	[more]
A0A5D3CL00	3.5e-69	71.05	(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3BSP4	3.5e-69	71.05	(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=...	[more]

BLAST of Moc08g40150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	5.3e-46	48.68	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51930.1	5.5e-35	45.99	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	1.5e-32	42.86	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	4.4e-32	47.77	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.2	4.4e-32	47.77	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 43..162 e-value: 4.7E-50 score: 172.9
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 27..185
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 44..158 e-value: 2.1E-9 score: 37.2
None	No IPR available	GENE3D	3.30.410.40		coord: 163..192 e-value: 4.7E-50 score: 172.9
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 4..193
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 4..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g40150.1	Moc08g40150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors