Moc08g37250 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g37250
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Reverse transcriptase domain-containing protein
Location	chr8: 27741825 .. 27743168 (+)
RNA-Seq Expression	Moc08g37250
Synteny	Moc08g37250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACCCCCTAGGTAATAGTATCGTGCGCCTTCGAGGTAACATCAGGGAAATTTCTTAGTTATTTGGTGAATCGACAAGGCACGAATGTAGACCCAGCTAAAACAAAAGCTGTGCTCGATATGCCAGAGCCAACCTCAGCAAAAGAGTTTAAGTCATTTTTAGGAAAAACTTCCTACTTGAGAAGGTTCTTACCAGCCTGCGCTACCCTCCCCGCACGTGCCGATGTCCGCCTCGCTCCTCTATTTTCTTTTAGGCATATTCCACTAATTCCTTATTTTAAGTAAGGTATCATTCCGCCCCTATGAAATAGCACATTTTTTCGCAGGTGGATCTACCTCTCGTCTGAGATTTATGATATTTATTGCTTTAACACGTCCCGCTTTCGGGCCTACTGTCCTACGTATGCTTCCCTCTCCCGCTCTTCAAGCCTAAAAGCGCTCTTGGAATTAAGCACCTTTCCAATGCCTAATGCAGCTCTTGCTCTTTTTTTCTTTTTTGAAAACAGCTCCTTCGAGAAGAGAACTTTGTATTTTGGTAATTCTCTAGGAGTCATGTTTAACCTTGAATGCTATTAATAAATTCTACAGCAATAGTCCCTCGGACTCGGAAAGTAATAACGAAAATGGCTAACCCAATAGCTGATTCCACAGCTGCCACCGTTGGAACCGGGGAACACATTCACTACTGGGCATTGACATCTTAATGCGTTGCAATCTGGACATTGATCGATCTTTATGCTTCCCAGCAGCTATACAGGGAAACACGGGATGGAAGGCGGGCGGAAAAGGCTTGGCTTTGTTTCATAGAGTGATGTGAAACCTCCACAAAGAAAAAACTTCTTGGACTTCCTTATCCGTATGGCCGGCCAATCCGTCACAAACGGAGCGGGGCCAATTTCTCGTACTTGCTCAGTGTGCCCCCCTTTCGTCGAGTGCCCCGCCCGGTTCACTGGGCTGTTGGCCTACCAAGCACTTGCCCGCTGCTCCTGCCGCCTGCTTGGCGGGCTCTGCGCTCGTTATCCTTACTGTTCTCTCTGCTGGGGCCCCCCATGGCTCTAGCCATAAGCTATGGCAGCTCCAGCTCGCAACCACAGAGGCTCAGTGGTCAGGTCTGCCCCCATTCGAATTACGTTAGGAGGTCTTTCGCTTTTGTCAGATCTAGAGAGATACTACGAGTCCTCTGTCCCAGATTCCATCGCTGCGGCCAATTTCGAGACCCCGTTCCTGTAGGAGGTTTCTCCTACACTCCCATCAATACTGGCCGTCCAGCGAATGCAGAGTCAAGTGTAGCTCCTGACATCACGGAAAAGACAAAGCAGGGAATAGCTTCAACGGCGAAATGA

mRNA sequence

ATGAACCCCCTAGACCCAGCTAAAACAAAAGCTGTGCTCGATATGCCAGAGCCAACCTCAGCAAAAGAGTTTAAGTCATTTTTAGGAAAAACTTCCTACTTGAGAAGGTTCTTACCAGCCTGCGCTACCCTCCCCGCACGTGCCGATGTCCGCCTCGCTCCTCTATTTTCTTTTAGGCATATTCCACTAATTCCTTATTTTAACTGCCACCGTTGGAACCGGGGAACACATTCACTACTGGGCATTGACATCTTAATGCGTTGCAATCTGGACATTGATCGATCTTTATGCTTCCCAGCAGCTATACAGGGAAACACGGGATGGAAGGCGGGCGGAAAAGGCTTGGCTTTGTTTCATAGAGTGATTGTGCCCCCCTTTCGTCGAGTGCCCCGCCCGGTTCACTGGGCTGTTGGCCTACCAAGCACTTGCCCGCTGCTCCTGCCGCCTGCTTGGCGGGCTCTGCGCTCGTTATCCTTACTGTTCTCTCTGCTGGGGCCCCCCATGGCTCTAGCCATAAGCTATGGCAGCTCCAGCTCGCAACCACAGAGGCTCAGTGGTCAGGTCTGCCCCCATTCGAATTACGTTAGGAGGTCTTTCGCTTTTGTCAGATCTAGAGAGATACTACGAGTCCTCTGTCCCAGATTCCATCGCTGCGGCCAATTTCGAGACCCCGTTCCTGTAGGAGGTTTCTCCTACACTCCCATCAATACTGGCCGTCCAGCGAATGCAGAGTCAAGTGTAGCTCCTGACATCACGGAAAAGACAAAGCAGGGAATAGCTTCAACGGCGAAATGA

Coding sequence (CDS)

ATGAACCCCCTAGACCCAGCTAAAACAAAAGCTGTGCTCGATATGCCAGAGCCAACCTCAGCAAAAGAGTTTAAGTCATTTTTAGGAAAAACTTCCTACTTGAGAAGGTTCTTACCAGCCTGCGCTACCCTCCCCGCACGTGCCGATGTCCGCCTCGCTCCTCTATTTTCTTTTAGGCATATTCCACTAATTCCTTATTTTAACTGCCACCGTTGGAACCGGGGAACACATTCACTACTGGGCATTGACATCTTAATGCGTTGCAATCTGGACATTGATCGATCTTTATGCTTCCCAGCAGCTATACAGGGAAACACGGGATGGAAGGCGGGCGGAAAAGGCTTGGCTTTGTTTCATAGAGTGATTGTGCCCCCCTTTCGTCGAGTGCCCCGCCCGGTTCACTGGGCTGTTGGCCTACCAAGCACTTGCCCGCTGCTCCTGCCGCCTGCTTGGCGGGCTCTGCGCTCGTTATCCTTACTGTTCTCTCTGCTGGGGCCCCCCATGGCTCTAGCCATAAGCTATGGCAGCTCCAGCTCGCAACCACAGAGGCTCAGTGGTCAGGTCTGCCCCCATTCGAATTACGTTAGGAGGTCTTTCGCTTTTGTCAGATCTAGAGAGATACTACGAGTCCTCTGTCCCAGATTCCATCGCTGCGGCCAATTTCGAGACCCCGTTCCTGTAGGAGGTTTCTCCTACACTCCCATCAATACTGGCCGTCCAGCGAATGCAGAGTCAAGTGTAGCTCCTGACATCACGGAAAAGACAAAGCAGGGAATAGCTTCAACGGCGAAATGA

Protein sequence

MNPLDPAKTKAVLDMPEPTSAKEFKSFLGKTSYLRRFLPACATLPARADVRLAPLFSFRHIPLIPYFNCHRWNRGTHSLLGIDILMRCNLDIDRSLCFPAAIQGNTGWKAGGKGLALFHRVIVPPFRRVPRPVHWAVGLPSTCPLLLPPAWRALRSLSLLFSLLGPPMALAISYGSSSSQPQRLSGQVCPHSNYVRRSFAFVRSREILRVLCPRFHRCGQFRDPVPVGGFSYTPINTGRPANAESSVAPDITEKTKQGIASTAK

Homology

[Close]

BLAST of Moc08g37250 vs. NCBI nr
Match: KAF1898875.1 (hypothetical protein Lal_00018996 [Lupinus albus])

HSP 1 Score: 118.2 bits (295), Expect = 1.0e-22
Identity = 74/114 (64.91%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 117 LFHRVIVPPFRRVPRPVHWAVGLPSTCPLLLPPA--WRALRSLSLLFSLLG-PPMALAIS 176
           L + + VPPFRRVP PVHWAVGLPSTC LLLPPA   RA RSLSLLFS+ G PP+ALAIS
Sbjct: 97  LSYLLSVPPFRRVPHPVHWAVGLPSTCLLLLPPAKRKRAERSLSLLFSMSGPPPIALAIS 156

Query: 177 YGSSSSQPQ--------RLSGQVCPHSNYVRRSFAFVRSREILRVLCPRFHRCG 220
           YGSSSS+PQ         LSGQ            AF RSREILRVL PRFHR G
Sbjct: 157 YGSSSSKPQGPAPRGQSGLSGQPPIRITLRYGYLAFARSREILRVLRPRFHRRG 210

[Close]

BLAST of Moc08g37250 vs. NCBI nr
Match: KAF1002588.1 (hypothetical protein AG4045_005158 [Apium graveolens])

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-13
Identity = 41/43 (95.35%), Postives = 41/43 (95.35%), Query Frame = 0


Query: 125 PFRRVPRPVHWAVGLPSTCPLLLPPAWRALRSLSLLFSLLGPP 168
           PFRRVPRPVHWAVGLPSTCPLLLPPAWRA RSLSLLFSL GPP
Sbjct: 328 PFRRVPRPVHWAVGLPSTCPLLLPPAWRAERSLSLLFSLPGPP 370

[Close]

BLAST of Moc08g37250 vs. NCBI nr
Match: KAF3949771.1 (hypothetical protein CMV_024397 [Castanea mollissima])

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-10
Identity = 49/66 (74.24%), Postives = 50/66 (75.76%), Query Frame = 0


Query: 150 AWRALRSLSLLFSLLG-PPMALAISYGSSSSQPQ--------RLSGQVCPHSNYVRRSFA 207
           A RA RSLSLLFSL G PP+ALAISYGSSSSQPQ         LSGQV  HSNYVRRSFA
Sbjct: 15  AKRAERSLSLLFSLPGPPPIALAISYGSSSSQPQGPALRGQSGLSGQVSLHSNYVRRSFA 74

[Close]

BLAST of Moc08g37250 vs. NCBI nr
Match: KAF3949772.1 (hypothetical protein CMV_024397 [Castanea mollissima])

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-10
Identity = 49/66 (74.24%), Postives = 50/66 (75.76%), Query Frame = 0


Query: 150 AWRALRSLSLLFSLLG-PPMALAISYGSSSSQPQ--------RLSGQVCPHSNYVRRSFA 207
           A RA RSLSLLFSL G PP+ALAISYGSSSSQPQ         LSGQV  HSNYVRRSFA
Sbjct: 69  AKRAERSLSLLFSLPGPPPIALAISYGSSSSQPQGPALRGQSGLSGQVSLHSNYVRRSFA 128

[Close]

BLAST of Moc08g37250 vs. NCBI nr
Match: KAG6384682.1 (hypothetical protein SASPL_155539 [Salvia splendens])

HSP 1 Score: 73.2 bits (178), Expect = 3.9e-09
Identity = 35/38 (92.11%), Postives = 35/38 (92.11%), Query Frame = 0


Query: 129 VPRPVHWAVGLPSTCPLLLPPAWRALRSLSLLFSLLGP 167
           VPRPVHWAVGLPSTCPL LPPAWRA RSLSLL SLLGP
Sbjct: 185 VPRPVHWAVGLPSTCPLPLPPAWRAERSLSLLCSLLGP 222

[Close]

BLAST of Moc08g37250 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.0e-04
Identity = 20/34 (58.82%), Postives = 25/34 (73.53%), Query Frame = 0


Query: 5   DPAKTKAVLDMPEPTSAKEFKSFLGKTSYLRRFL 39
           DP K +A+ +MP PTS KE K FLG TSY R+F+
Sbjct: 339 DPKKVRAISEMPPPTSVKELKRFLGMTSYYRKFI 372

[Close]

BLAST of Moc08g37250 vs. ExPASy TrEMBL
Match: A0A6A5PLN5 (Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00018996 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 5.1e-23
Identity = 74/114 (64.91%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 117 LFHRVIVPPFRRVPRPVHWAVGLPSTCPLLLPPA--WRALRSLSLLFSLLG-PPMALAIS 176
           L + + VPPFRRVP PVHWAVGLPSTC LLLPPA   RA RSLSLLFS+ G PP+ALAIS
Sbjct: 97  LSYLLSVPPFRRVPHPVHWAVGLPSTCLLLLPPAKRKRAERSLSLLFSMSGPPPIALAIS 156

Query: 177 YGSSSSQPQ--------RLSGQVCPHSNYVRRSFAFVRSREILRVLCPRFHRCG 220
           YGSSSS+PQ         LSGQ            AF RSREILRVL PRFHR G
Sbjct: 157 YGSSSSKPQGPAPRGQSGLSGQPPIRITLRYGYLAFARSREILRVLRPRFHRRG 210

[Close]

BLAST of Moc08g37250 vs. ExPASy TrEMBL
Match: A0A6L5BAR3 (Uncharacterized protein OS=Apium graveolens OX=4045 GN=AG4045_005158 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 7.3e-14
Identity = 41/43 (95.35%), Postives = 41/43 (95.35%), Query Frame = 0


Query: 125 PFRRVPRPVHWAVGLPSTCPLLLPPAWRALRSLSLLFSLLGPP 168
           PFRRVPRPVHWAVGLPSTCPLLLPPAWRA RSLSLLFSL GPP
Sbjct: 328 PFRRVPRPVHWAVGLPSTCPLLLPPAWRAERSLSLLFSLPGPP 370

[Close]

BLAST of Moc08g37250 vs. ExPASy TrEMBL
Match: A0A2N9EGN9 (CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS1722 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 4.7e-13
Identity = 46/62 (74.19%), Postives = 47/62 (75.81%), Query Frame = 0


Query: 166 PPMALAISYGSSSSQPQ--------RLSGQVCPHSNYVRRSFAFVRSREILRVLCPRFHR 220
           PP  LA+SYGSSSSQPQ         LSGQV PHSNYVRRSFAF RSREILRVL PRFHR
Sbjct: 89  PPSELALSYGSSSSQPQGPALRGQSGLSGQVSPHSNYVRRSFAFARSREILRVLRPRFHR 148

[Close]

BLAST of Moc08g37250 vs. ExPASy TrEMBL
Match: A0A2N9FMS5 (CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS16345 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 4.7e-13
Identity = 46/62 (74.19%), Postives = 47/62 (75.81%), Query Frame = 0


Query: 166 PPMALAISYGSSSSQPQ--------RLSGQVCPHSNYVRRSFAFVRSREILRVLCPRFHR 220
           PP  LA+SYGSSSSQPQ         LSGQV PHSNYVRRSFAF RSREILRVL PRFHR
Sbjct: 55  PPSELALSYGSSSSQPQGPALRGQSGLSGQVSPHSNYVRRSFAFARSREILRVLRPRFHR 114

[Close]

BLAST of Moc08g37250 vs. ExPASy TrEMBL
Match: A0A2Z2C4V2 (Reverse transcriptase domain-containing protein OS=Cocos nucifera OX=13894 GN=orf135c PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 5.4e-09
Identity = 35/43 (81.40%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 4   LDPAKTKAVLDMPEPTSAKEFKSFLGKTSYLRRFLPACATLPA 47
           +DPAK KAVLDMPEPTSAKE KSFLGK SYLRRFLP  A L A
Sbjct: 232 VDPAKAKAVLDMPEPTSAKELKSFLGKASYLRRFLPGLAALSA 274

The following BLAST results are available for this feature:

BLAST of Moc08g37250 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF1898875.1	1.0e-22	64.91	hypothetical protein Lal_00018996 [Lupinus albus]	[more]
KAF1002588.1	1.5e-13	95.35	hypothetical protein AG4045_005158 [Apium graveolens]	[more]
KAF3949771.1	2.0e-10	74.24	hypothetical protein CMV_024397 [Castanea mollissima]	[more]
KAF3949772.1	2.0e-10	74.24	hypothetical protein CMV_024397 [Castanea mollissima]	[more]
KAG6384682.1	3.9e-09	92.11	hypothetical protein SASPL_155539 [Salvia splendens]	[more]

BLAST of Moc08g37250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8I7P9	3.0e-04	58.82	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]

BLAST of Moc08g37250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6A5PLN5	5.1e-23	64.91	Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00018996 PE=4 SV=1	[more]
A0A6L5BAR3	7.3e-14	95.35	Uncharacterized protein OS=Apium graveolens OX=4045 GN=AG4045_005158 PE=3 SV=1	[more]
A0A2N9EGN9	4.7e-13	74.19	CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS1722...	[more]
A0A2N9FMS5	4.7e-13	74.19	CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS1634...	[more]
A0A2Z2C4V2	5.4e-09	81.40	Reverse transcriptase domain-containing protein OS=Cocos nucifera OX=13894 GN=or...	[more]

BLAST of Moc08g37250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 7..59 e-value: 1.4E-8 score: 36.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 241..264
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 4..44

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g37250.1	Moc08g37250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046034	ATP metabolic process
biological_process	GO:1902600	proton transmembrane transport
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0031090	organelle membrane
molecular_function	GO:0005524	ATP binding