Moc08g36310 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g36310
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionTHO complex subunit 4D-like
Locationchr8: 26924789 .. 26928093 (-)
RNA-Seq ExpressionMoc08g36310
SyntenyMoc08g36310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGTGGGAAAAGTGGTGGGCTGGGCGGCTGCAACAAGAGGGAGGGAGAGAAGAAGAAGGAGAAAGGGAAGGTAGGGTTTTGGTTTCTTTTAATTTTTTTTTCTTTTTCCCTTTTCGTTTTCCTTTCTTTTCTTTTTTTACCAAAAGAAATAATAAACCAAAATAATGTACTCAAGACATTAAACAATAGCGTAAATCTTCATCCATTTTTGTTAAACCAATAATAAAATCAAAAAGGAAAAATTGATTTACAAAAACTTTGAATTAAGATTTAATTAAAGATCTCTTTCCAAAAATTTGAAAACTTAATTTATCATAATTCGATAAAATTAAACTTAATTTTAATTTATCCAAAATTAGAACTCTAAATTTAGAAACTATAAGAATTTAAATATCAGCTTAGGAAAAAATCAAGAACGCGGGGCGTTACAAACTACCCAAAAAAAACTTTCTTCCTCGAAAGTTCGAGATCGCACTACTCTAAATGTCGGGGCATTACAAAAGTCTGTCACAATTTCAATCTAATTGGAGAGAGTCATTTCAATTTCATCTCTAAACTATATGGGGGATTGCGAACGACAAACCTACGGAGCTCAGTGATGGCAATGAGGCTGGGCGAAAAATGCAATTTATTTACTATTTAAAGAAATTGTAAAAACCTTTAGTTTGGTCATTATTTTAAAAAAACAAAAATTTTAAAACTTTTTAATTTAAAAACAAGAGTATACTTTTATAAATAACATATCTCCATAATTAGGAAATTCCACTTTTCTCGACTCTTTCTCAAAAAAGTCGAGAATTGTAATATTTGAACAAAAAAATTAAAAATAATAGCGTCCAACAATTTATTAAAATATTCGTATAAAGTGTATTATGCCGACTTTTTTCTAAAAATAGTCGGGAGTGGTAATTTAAAAAAAAAATTAAGAATAAACTTTGTCCAACTATATATTAAAATAGTCCGGAATATAAACCTCTCCCAAAATTTCAAAACCCGTCAAAAGTGGTAATATATAAAAACAAAATTTTTATCGGAAAAAACTCATAATTCTTGTATCGTTGGCAATTGAATATATGAATTTGGATCATCAAGGTAACGAATCTTGAACAACGGCACGAGGAAGATGATGTTTGGTGTGTCCATAATTGAAGTCCATTGGGCTAAACTCGGGACCGAGTAGGAAGGGGACAATTATGCATGGTAAGAAGCAGATCACTATACCAAATACAATTTTCTACTTATTTCATGAGTATTTGGGGAAACTATTAATCATAAATCTATACTCGATACAACCGGAGGATCCCGAGTAACTAATATCATCCATAAAACTACAGTTTTTCCGACTGAAATTAAACGTGCTTAAAATCATTTGTGACACTTTTTGTATGTCGTGGATTCTCAAGATGGTTTAGGTAACTGTCACAGAATACTTGAAATTTGTTTATTTTTATTGACAATTATAAAATGTCATAAAAGATGAAAATTTTCGTAAATTGTCGTTTTTTTCCGCCAAAATCGCGCCTCCACCATTTTGTGACAATTTTTCACTGTCATTGCAAATTCGGTTTTCATGACAGTTTTTAACCGTCATAAAACTTACAAAATCCGAAAAATAACGTTTTTTCCCGTCAAAAACGCGCCCCCATCATTTATGAAGTTTTTTTTTCTCCAAACTTTTTTACTGTTCACAACTGTGATGGAAGACCACCTTTCTACCGGTGAGCTTTGTTGAAGATGGATGTTTTTGTTGAAGATGGATGTTGAGTAGGACGATGAGGTAATTTTCAAGTAATATTGAATACAGTTTGATACATCAAATGAAGTATTGATTTATTAACTTCCATGCAAGTAACGTGTGATGTTTATCTTACCTTTCTGCAGGACTTCTTTTTTTATCATTCTATTTCTTCTTATACAAACCAATGGCTACGCCTGTGGATATCTCACGAGAGGCTGTGATAAAGAAGAATAACCGTAAGAAGCTCAGAGCACGACGTAGGGCCCGTTGTGGGCGTGGAGCAGGTGGGTCTTTTAATGGTGGAAGAGTAGTGATAGGGTCGGTTCGTAATCTCAACATAAATGCACGACCATCTGCTTGCTCAATTAGCTTTAAACTGTCGATATTTAATGCATATATATGCTTGAGAACCTGATATTCCTTGAAAATACATGCTCATGGGGATCTCAAATAACATTACTATAGTAACTCTAAAAGAAGATGATCAGTAAAATTGTTGTTCTATGGAGAATCTCATTGCGGCTCACTCTCTTGTCGGAATCGTCATCTGGTGTATTTGTACCATTATCTTTGCAAATATTGAATACATGTGGGTTATATGGGTAACTTTCAAAGGACTGTCAAGAGTGGAGAATTATATTATTGTCGATTAATAAGCTGATATGTGTTTTCCCCATCTGGATTCTTATCAGCATGCTTTCATCAGCCCCCACGCAGAATGAAGAATGTTCAATGGCAGCATGATTTACTTGAAGACAGTCTTAGAGCTTCAGGGATTTCTGGAATTGAAATTGGCACAAAGTTGTACGTTTCCGACTTGAATTTTGGGGTGACCAAAGAAGACATAATGGTACAACTTAAGCCATATATATTTGACTAAAGATGATTTCGATTAATATTCTCTTGTAACCTTACGGTGAACTCAGGAGTTGTTCTCTGCTGTTGGAGACCTGAAAAGATTTGCAATTCATTATGACAAAAATGGCCGTCCATGTGTAAGTTCCTACACTATCTTTTTGTAAAACTTTCCAGTAATGTGTTGCTGTTTGGCTTTTTAGTTTTTCCCATATCAAGTATCAATGTTATTTTTGTAGTATAGACTCACAAAAATGGAGTGAGAGAATTCGAACTCATGATCTTTTGGTCAGAAATAGGTGTTAATTACCACTGAGCTGAGCTAAGTTCATATTGTTGATACTAGATTACATGTTATGAATTCAAAAACCAACTTCTGCTGAATGTAGGTTTAAACCCGTTGGTTTGGTAAGTTCTTTAGATTTACTTGTTCACCCCTAATTCATATTTAGTTTCTTTTAAGTTGACATGGAATTTATCCGCAAATATGATAATTGTTGTACTTGGGCTTAAAATGTGTTCTTAATTGGATTATGGATGGTTCTCTTAACAAGCTTTAGTTGGCTGATAAACTTTCCATTGTTCATTAAAGGGCTTGGCAGAGGTGGTATATACTCGTAGAAGTGATGCATTTGCTGCTCAGAAGCGCTATAACAATGTGTTACTGGATGGGAAGCCATTGAAGATTGAAATTCTTGGTAACAACTAA

mRNA sequence

ATGCTGTGGGAAAAGTGGTGGGCTGGGCGGCTGCAACAAGAGGGAGGGAGAGAAGAAGAAGGAGAAAGGGAAGGACTTCTTTTTTTATCATTCTATTTCTTCTTATACAAACCAATGGCTACGCCTGTGGATATCTCACGAGAGGCTGTGATAAAGAAGAATAACCGTAAGAAGCTCAGAGCACGACGTAGGGCCCGTTGTGGGCGTGGAGCAGCATGCTTTCATCAGCCCCCACGCAGAATGAAGAATGTTCAATGGCAGCATGATTTACTTGAAGACAGTCTTAGAGCTTCAGGGATTTCTGGAATTGAAATTGGCACAAAGTTGTACGTTTCCGACTTGAATTTTGGGGTGACCAAAGAAGACATAATGGAGTTGTTCTCTGCTGTTGGAGACCTGAAAAGATTTGCAATTCATTATGACAAAAATGGCCGTCCATGTGGCTTGGCAGAGGTGGTATATACTCGTAGAAGTGATGCATTTGCTGCTCAGAAGCGCTATAACAATGTGTTACTGGATGGGAAGCCATTGAAGATTGAAATTCTTGGTAACAACTAA

Coding sequence (CDS)

ATGCTGTGGGAAAAGTGGTGGGCTGGGCGGCTGCAACAAGAGGGAGGGAGAGAAGAAGAAGGAGAAAGGGAAGGACTTCTTTTTTTATCATTCTATTTCTTCTTATACAAACCAATGGCTACGCCTGTGGATATCTCACGAGAGGCTGTGATAAAGAAGAATAACCGTAAGAAGCTCAGAGCACGACGTAGGGCCCGTTGTGGGCGTGGAGCAGCATGCTTTCATCAGCCCCCACGCAGAATGAAGAATGTTCAATGGCAGCATGATTTACTTGAAGACAGTCTTAGAGCTTCAGGGATTTCTGGAATTGAAATTGGCACAAAGTTGTACGTTTCCGACTTGAATTTTGGGGTGACCAAAGAAGACATAATGGAGTTGTTCTCTGCTGTTGGAGACCTGAAAAGATTTGCAATTCATTATGACAAAAATGGCCGTCCATGTGGCTTGGCAGAGGTGGTATATACTCGTAGAAGTGATGCATTTGCTGCTCAGAAGCGCTATAACAATGTGTTACTGGATGGGAAGCCATTGAAGATTGAAATTCTTGGTAACAACTAA

Protein sequence

MLWEKWWAGRLQQEGGREEEGEREGLLFLSFYFFLYKPMATPVDISREAVIKKNNRKKLRARRRARCGRGAACFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN
Homology
BLAST of Moc08g36310 vs. NCBI nr
Match: XP_022143315.1 (THO complex subunit 4D-like [Momordica charantia])

HSP 1 Score: 272.3 bits (695), Expect = 3.0e-69
Identity = 144/178 (80.90%), Postives = 144/178 (80.90%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGA------------------------AC- 98
           MATPVDISREAVIKKNNRKKLRARRRARCGRGA                        AC 
Sbjct: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS 60

Query: 99  ------FHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF 158
                    PPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF
Sbjct: 61  ISFKLSIFNPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF 120

Query: 159 SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN
Sbjct: 121 SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 178

BLAST of Moc08g36310 vs. NCBI nr
Match: XP_022143379.1 (THO complex subunit 4D-like [Momordica charantia])

HSP 1 Score: 233.0 bits (593), Expect = 2.0e-57
Identity = 122/174 (70.11%), Postives = 133/174 (76.44%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAA-------------------------- 98
           MATP+D+S E +IKKNNR+KLRAR RAR GRGA                           
Sbjct: 1   MATPLDMSLEDMIKKNNREKLRARGRARRGRGAGGSFNGGRVVIGSIRRGPLSINTRPSA 60

Query: 99  -CFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVG 158
               +PPRRMKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +G
Sbjct: 61  FSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEIG 120

Query: 159 DLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           DLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIEILG+N
Sbjct: 121 DLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEILGDN 174

BLAST of Moc08g36310 vs. NCBI nr
Match: XP_022935328.1 (THO complex subunit 4D-like [Cucurbita moschata] >XP_022935329.1 THO complex subunit 4D-like [Cucurbita moschata] >XP_022935330.1 THO complex subunit 4D-like [Cucurbita moschata])

HSP 1 Score: 232.6 bits (592), Expect = 2.7e-57
Identity = 122/175 (69.71%), Postives = 133/175 (76.00%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAA-------------------------- 98
           MATP+D+S E VIKKNNR+KLRAR RAR GRGA                           
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 99  --CFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 158
                +PPRRMKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 159 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

BLAST of Moc08g36310 vs. NCBI nr
Match: KAG6581189.1 (THO complex subunit 4C, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 232.6 bits (592), Expect = 2.7e-57
Identity = 122/175 (69.71%), Postives = 133/175 (76.00%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAA-------------------------- 98
           MATP+D+S E VIKKNNR+KLRAR RAR GRGA                           
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGKAVVIGSVRRGPLGINARPS 60

Query: 99  --CFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 158
                +PPRRMKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 159 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

BLAST of Moc08g36310 vs. NCBI nr
Match: XP_023527122.1 (THO complex subunit 4D [Cucurbita pepo subsp. pepo])

HSP 1 Score: 232.6 bits (592), Expect = 2.7e-57
Identity = 122/175 (69.71%), Postives = 133/175 (76.00%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAA-------------------------- 98
           MATP+D+S E VIKKNNR+KLRAR RAR GRGA                           
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 99  --CFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 158
                +PPRRMKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 159 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

BLAST of Moc08g36310 vs. ExPASy Swiss-Prot
Match: Q6NQ72 (THO complex subunit 4D OS=Arabidopsis thaliana OX=3702 GN=ALY4 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.9e-37
Identity = 77/142 (54.23%), Postives = 101/142 (71.13%), Query Frame = 0

Query: 60  RARRRARCGRGAA----------------CFHQPPRRMKNVQWQHDLLEDSLRASGISGI 119
           R R R   GRGA                   ++P RR++++ WQ  L ED LRA+G SG+
Sbjct: 30  RGRGRGGGGRGAGPARRGPLAVNARPSSFTINKPVRRVRSLPWQSGLFEDGLRAAGASGV 89

Query: 120 EIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAA 179
           E+GT+L+V++L+ GVT EDI ELFS +G+++R+AIHYDKNGRP G AEVVY RRSDAF A
Sbjct: 90  EVGTRLHVTNLDQGVTNEDIRELFSEIGEVERYAIHYDKNGRPSGTAEVVYPRRSDAFQA 149

Query: 180 QKRYNNVLLDGKPLKIEILGNN 186
            K+YNNVLLDG+P+++EILG N
Sbjct: 150 LKKYNNVLLDGRPMRLEILGGN 171

BLAST of Moc08g36310 vs. ExPASy Swiss-Prot
Match: Q94EH8 (THO complex subunit 4C OS=Arabidopsis thaliana OX=3702 GN=ALY3 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-35
Identity = 67/109 (61.47%), Postives = 91/109 (83.49%), Query Frame = 0

Query: 79  RRMKNVQW--QHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRF 138
           RR +++ W  Q+DL E++LRA G+SG+E+GT +Y+++L+ GVT EDI EL++ +G+LKR+
Sbjct: 77  RRKRSLPWQNQNDLYEETLRAVGVSGVEVGTTVYITNLDQGVTNEDIRELYAEIGELKRY 136

Query: 139 AIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           AIHYDKNGRP G AEVVY RRSDA  A ++YNNVLLDG+P+K+EILG N
Sbjct: 137 AIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDGRPMKLEILGGN 185

BLAST of Moc08g36310 vs. ExPASy Swiss-Prot
Match: Q8L773 (THO complex subunit 4A OS=Arabidopsis thaliana OX=3702 GN=ALY1 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.4e-29
Identity = 73/168 (43.45%), Postives = 98/168 (58.33%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAACFHQPPRR-----------------M 98
           M+T +D+S + +I KN  +K R       G G+     P RR                  
Sbjct: 1   MSTGLDMSLDDMIAKN--RKSRGGAGPARGTGSGSGPGPTRRNNPNRKSTRSAPYQSAKA 60

Query: 99  KNVQWQHDLL----EDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRFA 158
               W HD+     ED       +GIE GTKLY+S+L++GV  EDI ELF+ VG+LKR+ 
Sbjct: 61  PESTWGHDMFSDRSEDHRSGRSSAGIETGTKLYISNLDYGVMNEDIKELFAEVGELKRYT 120

Query: 159 IHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           +H+D++GR  G AEVVY+RR DA AA K+YN+V LDGKP+KIEI+G N
Sbjct: 121 VHFDRSGRSKGTAEVVYSRRGDALAAVKKYNDVQLDGKPMKIEIVGTN 166

BLAST of Moc08g36310 vs. ExPASy Swiss-Prot
Match: Q8L719 (THO complex subunit 4B OS=Arabidopsis thaliana OX=3702 GN=ALY2 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.5e-28
Identity = 68/134 (50.75%), Postives = 91/134 (67.91%), Query Frame = 0

Query: 66  RCGRGAACFHQPPRRMK--NVQWQHDLL--EDSLRAS----------GISGIEIGTKLYV 125
           R G   A + +P ++ +  +  WQ+D+   + S+ A+          G S IE GTKLY+
Sbjct: 53  RVGARTAPYSRPIQQQQAHDAMWQNDVFATDASVAAAFGHHQTAVVGGGSSIETGTKLYI 112

Query: 126 SDLNFGVTKEDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVL 185
           S+L++GV+ EDI ELFS VGDLKR+ IHYD++GR  G AEVV++RR DA AA KRYNNV 
Sbjct: 113 SNLDYGVSNEDIKELFSEVGDLKRYGIHYDRSGRSKGTAEVVFSRRGDALAAVKRYNNVQ 172

BLAST of Moc08g36310 vs. ExPASy Swiss-Prot
Match: Q3T0I4 (THO complex subunit 4 OS=Bos taurus OX=9913 GN=ALYREF PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 2.9e-22
Identity = 50/115 (43.48%), Postives = 77/115 (66.96%), Query Frame = 0

Query: 68  GRGAACFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF 127
           GR     +  P+++ + +WQHDL +      G +G+E G KL VS+L+FGV+  DI ELF
Sbjct: 70  GRNRPAPYSRPKQLPD-KWQHDLFDSGF--GGGAGVETGGKLLVSNLDFGVSDADIQELF 129

Query: 128 SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEIL 183
           +  G LK+ A+HYD++GR  G A+V + R++DA  A K+YN V LDG+P+ I+++
Sbjct: 130 AEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLV 181

BLAST of Moc08g36310 vs. ExPASy TrEMBL
Match: A0A6J1CNH0 (THO complex subunit 4D-like OS=Momordica charantia OX=3673 GN=LOC111013215 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.5e-69
Identity = 144/178 (80.90%), Postives = 144/178 (80.90%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGA------------------------AC- 98
           MATPVDISREAVIKKNNRKKLRARRRARCGRGA                        AC 
Sbjct: 1   MATPVDISREAVIKKNNRKKLRARRRARCGRGAGGSFNGGRVVIGSVRNLNINARPSACS 60

Query: 99  ------FHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF 158
                    PPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF
Sbjct: 61  ISFKLSIFNPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELF 120

Query: 159 SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN
Sbjct: 121 SAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 178

BLAST of Moc08g36310 vs. ExPASy TrEMBL
Match: A0A6J1CP51 (THO complex subunit 4D-like OS=Momordica charantia OX=3673 GN=LOC111013262 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 9.9e-58
Identity = 122/174 (70.11%), Postives = 133/174 (76.44%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAA-------------------------- 98
           MATP+D+S E +IKKNNR+KLRAR RAR GRGA                           
Sbjct: 1   MATPLDMSLEDMIKKNNREKLRARGRARRGRGAGGSFNGGRVVIGSIRRGPLSINTRPSA 60

Query: 99  -CFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVG 158
               +PPRRMKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +G
Sbjct: 61  FSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEIG 120

Query: 159 DLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           DLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIEILG+N
Sbjct: 121 DLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEILGDN 174

BLAST of Moc08g36310 vs. ExPASy TrEMBL
Match: A0A6J1J564 (THO complex subunit 4D-like OS=Cucurbita maxima OX=3661 GN=LOC111481464 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.3e-57
Identity = 122/175 (69.71%), Postives = 133/175 (76.00%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAA-------------------------- 98
           MATP+D+S E VIKKNNR+KLRAR RAR GRGA                           
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 99  --CFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 158
                +PPRRMKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 159 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

BLAST of Moc08g36310 vs. ExPASy TrEMBL
Match: A0A6J1FAB9 (THO complex subunit 4D-like OS=Cucurbita moschata OX=3662 GN=LOC111442248 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.3e-57
Identity = 122/175 (69.71%), Postives = 133/175 (76.00%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAA-------------------------- 98
           MATP+D+S E VIKKNNR+KLRAR RAR GRGA                           
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 99  --CFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 158
                +PPRRMKNVQWQHDL EDSLRASGISGIEIGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 159 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

BLAST of Moc08g36310 vs. ExPASy TrEMBL
Match: A0A1S3C452 (THO complex subunit 4D OS=Cucumis melo OX=3656 GN=LOC103496802 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 2.4e-56
Identity = 119/175 (68.00%), Postives = 131/175 (74.86%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAA-------------------------- 98
           M TP+D+S E VIKKNNR+KLRAR RAR GRGA                           
Sbjct: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60

Query: 99  --CFHQPPRRMKNVQWQHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAV 158
                +PP RMKNVQWQHDL EDSLRASGISGI+IGTKLYVS+L++GVTKEDI ELFS +
Sbjct: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 159 GDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           GDLKRFAIHYDKNGRP G AEVVYTRRSDAFAA KRYNNVLLDGKP+KIE+LG+N
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN 175

BLAST of Moc08g36310 vs. TAIR 10
Match: AT5G37720.1 (ALWAYS EARLY 4 )

HSP 1 Score: 157.1 bits (396), Expect = 1.3e-38
Identity = 77/142 (54.23%), Postives = 101/142 (71.13%), Query Frame = 0

Query: 60  RARRRARCGRGAA----------------CFHQPPRRMKNVQWQHDLLEDSLRASGISGI 119
           R R R   GRGA                   ++P RR++++ WQ  L ED LRA+G SG+
Sbjct: 30  RGRGRGGGGRGAGPARRGPLAVNARPSSFTINKPVRRVRSLPWQSGLFEDGLRAAGASGV 89

Query: 120 EIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAA 179
           E+GT+L+V++L+ GVT EDI ELFS +G+++R+AIHYDKNGRP G AEVVY RRSDAF A
Sbjct: 90  EVGTRLHVTNLDQGVTNEDIRELFSEIGEVERYAIHYDKNGRPSGTAEVVYPRRSDAFQA 149

Query: 180 QKRYNNVLLDGKPLKIEILGNN 186
            K+YNNVLLDG+P+++EILG N
Sbjct: 150 LKKYNNVLLDGRPMRLEILGGN 171

BLAST of Moc08g36310 vs. TAIR 10
Match: AT5G37720.2 (ALWAYS EARLY 4 )

HSP 1 Score: 157.1 bits (396), Expect = 1.3e-38
Identity = 77/142 (54.23%), Postives = 101/142 (71.13%), Query Frame = 0

Query: 60  RARRRARCGRGAA----------------CFHQPPRRMKNVQWQHDLLEDSLRASGISGI 119
           R R R   GRGA                   ++P RR++++ WQ  L ED LRA+G SG+
Sbjct: 30  RGRGRGGGGRGAGPARRGPLAVNARPSSFTINKPVRRVRSLPWQSGLFEDGLRAAGASGV 89

Query: 120 EIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRFAIHYDKNGRPCGLAEVVYTRRSDAFAA 179
           E+GT+L+V++L+ GVT EDI ELFS +G+++R+AIHYDKNGRP G AEVVY RRSDAF A
Sbjct: 90  EVGTRLHVTNLDQGVTNEDIRELFSEIGEVERYAIHYDKNGRPSGTAEVVYPRRSDAFQA 149

Query: 180 QKRYNNVLLDGKPLKIEILGNN 186
            K+YNNVLLDG+P+++EILG N
Sbjct: 150 LKKYNNVLLDGRPMRLEILGGN 171

BLAST of Moc08g36310 vs. TAIR 10
Match: AT1G66260.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.2e-36
Identity = 67/109 (61.47%), Postives = 91/109 (83.49%), Query Frame = 0

Query: 79  RRMKNVQW--QHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRF 138
           RR +++ W  Q+DL E++LRA G+SG+E+GT +Y+++L+ GVT EDI EL++ +G+LKR+
Sbjct: 77  RRKRSLPWQNQNDLYEETLRAVGVSGVEVGTTVYITNLDQGVTNEDIRELYAEIGELKRY 136

Query: 139 AIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           AIHYDKNGRP G AEVVY RRSDA  A ++YNNVLLDG+P+K+EILG N
Sbjct: 137 AIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDGRPMKLEILGGN 185

BLAST of Moc08g36310 vs. TAIR 10
Match: AT1G66260.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.2e-36
Identity = 67/109 (61.47%), Postives = 91/109 (83.49%), Query Frame = 0

Query: 79  RRMKNVQW--QHDLLEDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRF 138
           RR +++ W  Q+DL E++LRA G+SG+E+GT +Y+++L+ GVT EDI EL++ +G+LKR+
Sbjct: 77  RRKRSLPWQNQNDLYEETLRAVGVSGVEVGTTVYITNLDQGVTNEDIRELYAEIGELKRY 136

Query: 139 AIHYDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           AIHYDKNGRP G AEVVY RRSDA  A ++YNNVLLDG+P+K+EILG N
Sbjct: 137 AIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDGRPMKLEILGGN 185

BLAST of Moc08g36310 vs. TAIR 10
Match: AT5G59950.3 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 129.8 bits (325), Expect = 2.3e-30
Identity = 73/166 (43.98%), Postives = 98/166 (59.04%), Query Frame = 0

Query: 39  MATPVDISREAVIKKNNRKKLRARRRARCGRGAACFHQPPRR---------------MKN 98
           M+T +D+S + +I KN  +K R       G G+     P RR                  
Sbjct: 1   MSTGLDMSLDDMIAKN--RKSRGGAGPARGTGSGSGPGPTRRNNPNRKSTRSAPYQSAPE 60

Query: 99  VQWQHDLL----EDSLRASGISGIEIGTKLYVSDLNFGVTKEDIMELFSAVGDLKRFAIH 158
             W HD+     ED       +GIE GTKLY+S+L++GV  EDI ELF+ VG+LKR+ +H
Sbjct: 61  STWGHDMFSDRSEDHRSGRSSAGIETGTKLYISNLDYGVMNEDIKELFAEVGELKRYTVH 120

Query: 159 YDKNGRPCGLAEVVYTRRSDAFAAQKRYNNVLLDGKPLKIEILGNN 186
           +D++GR  G AEVVY+RR DA AA K+YN+V LDGKP+KIEI+G N
Sbjct: 121 FDRSGRSKGTAEVVYSRRGDALAAVKKYNDVQLDGKPMKIEIVGTN 164

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143315.13.0e-6980.90THO complex subunit 4D-like [Momordica charantia][more]
XP_022143379.12.0e-5770.11THO complex subunit 4D-like [Momordica charantia][more]
XP_022935328.12.7e-5769.71THO complex subunit 4D-like [Cucurbita moschata] >XP_022935329.1 THO complex sub... [more]
KAG6581189.12.7e-5769.71THO complex subunit 4C, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023527122.12.7e-5769.71THO complex subunit 4D [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q6NQ721.9e-3754.23THO complex subunit 4D OS=Arabidopsis thaliana OX=3702 GN=ALY4 PE=1 SV=1[more]
Q94EH81.7e-3561.47THO complex subunit 4C OS=Arabidopsis thaliana OX=3702 GN=ALY3 PE=1 SV=1[more]
Q8L7735.4e-2943.45THO complex subunit 4A OS=Arabidopsis thaliana OX=3702 GN=ALY1 PE=1 SV=1[more]
Q8L7193.5e-2850.75THO complex subunit 4B OS=Arabidopsis thaliana OX=3702 GN=ALY2 PE=1 SV=1[more]
Q3T0I42.9e-2243.48THO complex subunit 4 OS=Bos taurus OX=9913 GN=ALYREF PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CNH01.5e-6980.90THO complex subunit 4D-like OS=Momordica charantia OX=3673 GN=LOC111013215 PE=4 ... [more]
A0A6J1CP519.9e-5870.11THO complex subunit 4D-like OS=Momordica charantia OX=3673 GN=LOC111013262 PE=4 ... [more]
A0A6J1J5641.3e-5769.71THO complex subunit 4D-like OS=Cucurbita maxima OX=3661 GN=LOC111481464 PE=4 SV=... [more]
A0A6J1FAB91.3e-5769.71THO complex subunit 4D-like OS=Cucurbita moschata OX=3662 GN=LOC111442248 PE=4 S... [more]
A0A1S3C4522.4e-5668.00THO complex subunit 4D OS=Cucumis melo OX=3656 GN=LOC103496802 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G37720.11.3e-3854.23ALWAYS EARLY 4 [more]
AT5G37720.21.3e-3854.23ALWAYS EARLY 4 [more]
AT1G66260.11.2e-3661.47RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT1G66260.21.2e-3661.47RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT5G59950.32.3e-3043.98RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 108..180
e-value: 3.8E-16
score: 69.6
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 109..178
e-value: 4.2E-11
score: 42.5
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 107..184
score: 13.532493
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 104..183
e-value: 1.8E-18
score: 68.5
NoneNo IPR availablePANTHERPTHR19965RNA AND EXPORT FACTOR BINDING PROTEINcoord: 39..185
NoneNo IPR availablePANTHERPTHR19965:SF33THO COMPLEX SUBUNIT 4Ccoord: 39..185
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 101..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g36310.1Moc08g36310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding