Moc08g34620 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g34620
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	rRNA adenine N(6)-methyltransferase
Location	chr8: 25281585 .. 25282189 (-)
RNA-Seq Expression	Moc08g34620
Synteny	Moc08g34620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGAAGTTAAGTGGAAGAGGTTTCCCTTCGAGTGGCTTATCGACCTTAGCTTGCAATTCTACTGATAAGTTAGATACTTGTAGAACTTCCCCTTGATTATATATTTGGGTATCATTGGAAGTCATGGCTGTGAATTATTTGATAACTTGAAATGTAGATTGTTGAGATTATGATTGGTGTGTTTATCATTATGATCCATAGACTGGATTGCTAGCTGGAGCCATGATAATTTCTTTCATCTTTTGAAGTCTATTCCCTGTAGGTTTAAAATATTCATATCGTTCTTCTGCTTTTATCTCGGTTTTACCCTTTCAGATACAATGGATGCTGAAGCTCAAGACGATATTTCTCCGAGGCCTCGGGTTAAAGCCTCATTTTACCTAGGGTCAGAGACACATTTGGTCAATACAGCTAATAAAGGCACTCTCTCAGAGCAGTTGGTTTCTATGAAAGAGGAGAGCATGGCTATTTTGAAGGAGTACATAACCAAACATAATGTTCCTAACTATGTGCCGGACGAACTAGCTGAAGAAGTTTCCGAGGACGACGACGAGATCCCCAAAAAGCCTCAAGTGAAGTCAAAGAAGTCTAAATTAACATAA

mRNA sequence

ATGCTGAAGTTAAGTGGAAGAGGTTTCCCTTCGAGTGGCTTATCGACCTTAGCTTGCAATTCTACTGATAAGTTTAAAATATTCATATCGTTCTTCTGCTTTTATCTCGGTTTTACCCTTTCAGATACAATGGATGCTGAAGCTCAAGACGATATTTCTCCGAGGCCTCGGGTTAAAGCCTCATTTTACCTAGGGTCAGAGACACATTTGGTCAATACAGCTAATAAAGGCACTCTCTCAGAGCAGTTGGTTTCTATGAAAGAGGAGAGCATGGCTATTTTGAAGGAGTACATAACCAAACATAATGTTCCTAACTATGTGCCGGACGAACTAGCTGAAGAAGTTTCCGAGGACGACGACGAGATCCCCAAAAAGCCTCAAGTGAAGTCAAAGAAGTCTAAATTAACATAA

Coding sequence (CDS)

ATGCTGAAGTTAAGTGGAAGAGGTTTCCCTTCGAGTGGCTTATCGACCTTAGCTTGCAATTCTACTGATAAGTTTAAAATATTCATATCGTTCTTCTGCTTTTATCTCGGTTTTACCCTTTCAGATACAATGGATGCTGAAGCTCAAGACGATATTTCTCCGAGGCCTCGGGTTAAAGCCTCATTTTACCTAGGGTCAGAGACACATTTGGTCAATACAGCTAATAAAGGCACTCTCTCAGAGCAGTTGGTTTCTATGAAAGAGGAGAGCATGGCTATTTTGAAGGAGTACATAACCAAACATAATGTTCCTAACTATGTGCCGGACGAACTAGCTGAAGAAGTTTCCGAGGACGACGACGAGATCCCCAAAAAGCCTCAAGTGAAGTCAAAGAAGTCTAAATTAACATAA

Protein sequence

MLKLSGRGFPSSGLSTLACNSTDKFKIFISFFCFYLGFTLSDTMDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYITKHNVPNYVPDELAEEVSEDDDEIPKKPQVKSKKSKLT

Homology

[Close]

BLAST of Moc08g34620 vs. NCBI nr
Match: XP_008443784.1 (PREDICTED: uncharacterized protein LOC103487289 [Cucumis melo])

HSP 1 Score: 150.2 bits (378), Expect = 1.3e-32
Identity = 77/97 (79.38%), Postives = 86/97 (88.66%), Query Frame = 0


Query: 40  LSDTMDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYIT 99
           L D+MDA A++DI  RPRVK SF LGSE HLVNTANKGTLSEQL+SMKE+SMAILKEYIT
Sbjct: 3   LPDSMDAPAEEDIPLRPRVKVSFCLGSEIHLVNTANKGTLSEQLISMKEDSMAILKEYIT 62

Query: 100 KHNVPNYVPDELAEEVSEDDDEIPKKPQVKSKKSKLT 137
           KHNVPN VPDEL EE SEDDDEI +KPQVK+KK+K+T
Sbjct: 63  KHNVPNDVPDELVEEASEDDDEITEKPQVKAKKTKVT 99

[Close]

BLAST of Moc08g34620 vs. NCBI nr
Match: KAA0035148.1 (uncharacterized protein E6C27_scaffold57G002390 [Cucumis melo var. makuwa])

HSP 1 Score: 150.2 bits (378), Expect = 1.3e-32
Identity = 77/97 (79.38%), Postives = 86/97 (88.66%), Query Frame = 0


Query: 40  LSDTMDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYIT 99
           L D+MDA A++DI  RPRVK SF LGSE HLVNTANKGTLSEQL+SMKE+SMAILKEYIT
Sbjct: 15  LPDSMDAPAEEDIPLRPRVKVSFCLGSEIHLVNTANKGTLSEQLISMKEDSMAILKEYIT 74

Query: 100 KHNVPNYVPDELAEEVSEDDDEIPKKPQVKSKKSKLT 137
           KHNVPN VPDEL EE SEDDDEI +KPQVK+KK+K+T
Sbjct: 75  KHNVPNDVPDELVEEASEDDDEITEKPQVKAKKTKVT 111

[Close]

BLAST of Moc08g34620 vs. NCBI nr
Match: TYK29223.1 (uncharacterized protein E5676_scaffold1228G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 146.4 bits (368), Expect = 1.8e-31
Identity = 75/93 (80.65%), Postives = 83/93 (89.25%), Query Frame = 0


Query: 44  MDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYITKHNV 103
           MDA A++DI  RPRVK SF LGSE HLVNTANKGTLSEQL+SMKE+SMAILKEYITKHNV
Sbjct: 1   MDAPAEEDIPLRPRVKVSFCLGSEIHLVNTANKGTLSEQLISMKEDSMAILKEYITKHNV 60

Query: 104 PNYVPDELAEEVSEDDDEIPKKPQVKSKKSKLT 137
           PN VPDEL EE SEDDDEI +KPQVK+KK+K+T
Sbjct: 61  PNDVPDELVEEASEDDDEITEKPQVKAKKTKVT 93

[Close]

BLAST of Moc08g34620 vs. NCBI nr
Match: KAG7036645.1 (hypothetical protein SDJN02_00265, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 146.0 bits (367), Expect = 2.4e-31
Identity = 79/96 (82.29%), Postives = 85/96 (88.54%), Query Frame = 0


Query: 42  DTMDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYITKH 101
           D+MDA+AQ+ IS  PRVKASF LGSE HLVNTANKGTLSEQL+SMKEESMAILKEYITKH
Sbjct: 102 DSMDAQAQEVISRSPRVKASFRLGSEKHLVNTANKGTLSEQLISMKEESMAILKEYITKH 161

Query: 102 NVPNYVPDELAEEVSE-DDDEIPKKPQVKSKKSKLT 137
           NVPN VPDEL EE SE DDDE  +KPQVKSKK+KLT
Sbjct: 162 NVPNDVPDELLEEASEDDDDETTEKPQVKSKKTKLT 197

[Close]

BLAST of Moc08g34620 vs. NCBI nr
Match: KAG6606942.1 (putative E3 ubiquitin ligase SUD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 146.0 bits (367), Expect = 2.4e-31
Identity = 79/96 (82.29%), Postives = 85/96 (88.54%), Query Frame = 0


Query: 42  DTMDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYITKH 101
           D+MDA+AQ+ IS  PRVKASF LGSE HLVNTANKGTLSEQL+SMKEESMAILKEYITKH
Sbjct: 249 DSMDAQAQEVISRSPRVKASFRLGSEKHLVNTANKGTLSEQLISMKEESMAILKEYITKH 308

Query: 102 NVPNYVPDELAEEVSE-DDDEIPKKPQVKSKKSKLT 137
           NVPN VPDEL EE SE DDDE  +KPQVKSKK+KLT
Sbjct: 309 NVPNDVPDELLEEASEDDDDETTEKPQVKSKKTKLT 344

[Close]

BLAST of Moc08g34620 vs. ExPASy TrEMBL
Match: A0A5A7SX16 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G002390 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 6.2e-33
Identity = 77/97 (79.38%), Postives = 86/97 (88.66%), Query Frame = 0


Query: 40  LSDTMDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYIT 99
           L D+MDA A++DI  RPRVK SF LGSE HLVNTANKGTLSEQL+SMKE+SMAILKEYIT
Sbjct: 15  LPDSMDAPAEEDIPLRPRVKVSFCLGSEIHLVNTANKGTLSEQLISMKEDSMAILKEYIT 74

Query: 100 KHNVPNYVPDELAEEVSEDDDEIPKKPQVKSKKSKLT 137
           KHNVPN VPDEL EE SEDDDEI +KPQVK+KK+K+T
Sbjct: 75  KHNVPNDVPDELVEEASEDDDEITEKPQVKAKKTKVT 111

[Close]

BLAST of Moc08g34620 vs. ExPASy TrEMBL
Match: A0A1S3B8U1 (uncharacterized protein LOC103487289 OS=Cucumis melo OX=3656 GN=LOC103487289 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 6.2e-33
Identity = 77/97 (79.38%), Postives = 86/97 (88.66%), Query Frame = 0


Query: 40  LSDTMDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYIT 99
           L D+MDA A++DI  RPRVK SF LGSE HLVNTANKGTLSEQL+SMKE+SMAILKEYIT
Sbjct: 3   LPDSMDAPAEEDIPLRPRVKVSFCLGSEIHLVNTANKGTLSEQLISMKEDSMAILKEYIT 62

Query: 100 KHNVPNYVPDELAEEVSEDDDEIPKKPQVKSKKSKLT 137
           KHNVPN VPDEL EE SEDDDEI +KPQVK+KK+K+T
Sbjct: 63  KHNVPNDVPDELVEEASEDDDEITEKPQVKAKKTKVT 99

[Close]

BLAST of Moc08g34620 vs. ExPASy TrEMBL
Match: A0A5D3DZN8 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1228G00090 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 8.9e-32
Identity = 75/93 (80.65%), Postives = 83/93 (89.25%), Query Frame = 0


Query: 44  MDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYITKHNV 103
           MDA A++DI  RPRVK SF LGSE HLVNTANKGTLSEQL+SMKE+SMAILKEYITKHNV
Sbjct: 1   MDAPAEEDIPLRPRVKVSFCLGSEIHLVNTANKGTLSEQLISMKEDSMAILKEYITKHNV 60

Query: 104 PNYVPDELAEEVSEDDDEIPKKPQVKSKKSKLT 137
           PN VPDEL EE SEDDDEI +KPQVK+KK+K+T
Sbjct: 61  PNDVPDELVEEASEDDDEITEKPQVKAKKTKVT 93

[Close]

BLAST of Moc08g34620 vs. ExPASy TrEMBL
Match: A0A0A0M0V3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G679550 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 2.0e-31
Identity = 75/93 (80.65%), Postives = 82/93 (88.17%), Query Frame = 0


Query: 44  MDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYITKHNV 103
           MDA A++DI  RPRVK S  LGSETHLVNTANKGTLSEQL SMKE+SMAILKEYITKHNV
Sbjct: 1   MDAPAEEDIPLRPRVKVSLCLGSETHLVNTANKGTLSEQLTSMKEDSMAILKEYITKHNV 60

Query: 104 PNYVPDELAEEVSEDDDEIPKKPQVKSKKSKLT 137
           PN VPDEL EE SEDDDEI +KPQVK+KK+K+T
Sbjct: 61  PNDVPDELVEEASEDDDEITEKPQVKAKKTKVT 93

[Close]

BLAST of Moc08g34620 vs. ExPASy TrEMBL
Match: A0A6J1G9M3 (uncharacterized protein LOC111452065 OS=Cucurbita moschata OX=3662 GN=LOC111452065 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 7.6e-31
Identity = 78/94 (82.98%), Postives = 83/94 (88.30%), Query Frame = 0


Query: 44  MDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYITKHNV 103
           MDA+AQ+ IS  PRVKASF LGSE HLVNTANKGTLSEQL+SMKEESMAILKEYITKHNV
Sbjct: 1   MDAQAQEVISRSPRVKASFRLGSEKHLVNTANKGTLSEQLISMKEESMAILKEYITKHNV 60

Query: 104 PNYVPDELAEEVSE-DDDEIPKKPQVKSKKSKLT 137
           PN VPDEL EE SE DDDE  +KPQVKSKK+KLT
Sbjct: 61  PNDVPDELLEEASEDDDDETTEKPQVKSKKTKLT 94

[Close]

BLAST of Moc08g34620 vs. TAIR 10
Match: AT1G47820.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G47813.1); Has 29 Blast hits to 29 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 29; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 68.6 bits (166), Expect = 4.6e-12
Identity = 40/95 (42.11%), Postives = 60/95 (63.16%), Query Frame = 0


Query: 44  MDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYITKHNV 103
           M+ E+   +  +  VK  F LG+E++ +++    T+ +QLVSMKEESM ILK++ITKHNV
Sbjct: 1   MEIESHVSVPGKNLVKVEFKLGTESYTIDSIKGDTVLDQLVSMKEESMKILKDFITKHNV 60

Query: 104 P-NYVPDELAEEVSEDDDE------IPKKPQVKSK 132
           P + VPD++  +  E DD+       P KP  K+K
Sbjct: 61  PDDDVPDQILSDDEEGDDDDASPVICPVKPPKKTK 95

[Close]

BLAST of Moc08g34620 vs. TAIR 10
Match: AT1G47820.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G47813.1); Has 29 Blast hits to 29 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 29; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 68.6 bits (166), Expect = 4.6e-12
Identity = 40/95 (42.11%), Postives = 60/95 (63.16%), Query Frame = 0


Query: 44  MDAEAQDDISPRPRVKASFYLGSETHLVNTANKGTLSEQLVSMKEESMAILKEYITKHNV 103
           M+ E+   +  +  VK  F LG+E++ +++    T+ +QLVSMKEESM ILK++ITKHNV
Sbjct: 1   MEIESHVSVPGKNLVKVEFKLGTESYTIDSIKGDTVLDQLVSMKEESMKILKDFITKHNV 60

Query: 104 P-NYVPDELAEEVSEDDDE------IPKKPQVKSK 132
           P + VPD++  +  E DD+       P KP  K+K
Sbjct: 61  PDDDVPDQILSDDEEGDDDDASPVICPVKPPKKTK 95

The following BLAST results are available for this feature:

BLAST of Moc08g34620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008443784.1	1.3e-32	79.38	PREDICTED: uncharacterized protein LOC103487289 [Cucumis melo]	[more]
KAA0035148.1	1.3e-32	79.38	uncharacterized protein E6C27_scaffold57G002390 [Cucumis melo var. makuwa]	[more]
TYK29223.1	1.8e-31	80.65	uncharacterized protein E5676_scaffold1228G00090 [Cucumis melo var. makuwa]	[more]
KAG7036645.1	2.4e-31	82.29	hypothetical protein SDJN02_00265, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6606942.1	2.4e-31	82.29	putative E3 ubiquitin ligase SUD1, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Moc08g34620 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc08g34620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SX16	6.2e-33	79.38	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A1S3B8U1	6.2e-33	79.38	uncharacterized protein LOC103487289 OS=Cucumis melo OX=3656 GN=LOC103487289 PE=...	[more]
A0A5D3DZN8	8.9e-32	80.65	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A0A0M0V3	2.0e-31	80.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G679550 PE=4 SV=1	[more]
A0A6J1G9M3	7.6e-31	82.98	uncharacterized protein LOC111452065 OS=Cucurbita moschata OX=3662 GN=LOC1114520...	[more]

BLAST of Moc08g34620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47820.1	4.6e-12	42.11	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G47820.2	4.6e-12	42.11	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 109..136
None	No IPR available	PANTHER	PTHR37194	T2E6.7-RELATED	coord: 44..134
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..19 score: 6.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g34620.1	Moc08g34620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0000154	rRNA modification
molecular_function	GO:0008649	rRNA methyltransferase activity