Moc08g34430 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g34430
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	PMEI domain-containing protein
Location	chr8: 25173933 .. 25174808 (+)
RNA-Seq Expression	Moc08g34430
Synteny	Moc08g34430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTCAATAATGGCGTCATTACTAATTTCTTCCTCTTCCTCTTCCTCGCCCTTTCTTCCTTCTCTGCCAATGCCATTTGCGTCCCTCGCAACTCCTCCCAGTTTGTCTCGCCTGCCGCTTCCCTGATACACTCCTCCCCTCCTCCGCCACAATCGCCGCCGCAATCCCCTCCGCAATCGCCGCCGCAATCACCACCGCAAGCACCGCCGCAATCACCGCCGCAGTCACCGCCACAAGCACCGCCACAGTCTCCCCCACCGCAGAAGCAGTCGCCACCTCCTACACCGGTTTCCGACGATGATATCATCGGAAACGTGGAAGGCCCTCGAGCGGAAACCATGTTCGATGATATGATCGAGGCGATGTCGGAGATTCCGGAATTTGTACCAACGACGACGGAATGGAATGGGACGGATCCAATCGATCTCAGCAAGATCTGCAGCAACACGGACAACCCTCAGCTCTGTACCAAATCGATTTCAACTCACCTCAAGCCAGGAACGAACCAAATCGATCCTGTTTCGGCGCTGAAGACCGAAATTGGGGAATCGATTATCAAGGTGCAGAATGCAATGGAAAGAGCGCGCGAACTCCGCAAAGATCCATCGACATCGAAACTCGCAAACGTGTGCTTAGCTACTTGCCTTGACACTTACAATTTGGCGATCTTTGACCTAAAAAAAGTATTGGAATCGATCGAACATCACGACGCTGGTACAATGGAGAGCTACCTCAGCGCCGTACTCTCCAATATCGGGACCTGCGATGATTCTTTTGCAGAGATGGGCTTGGTTTCGCCATTGGATAATCTTGCAATCGAGCTGAACAAGTTCGCAAGCAATTGCTTGGCGATTTCCAAAATGCTTCTGTAA

mRNA sequence

ATGAAGCTCAATAATGGCGTCATTACTAATTTCTTCCTCTTCCTCTTCCTCGCCCTTTCTTCCTTCTCTGCCAATGCCATTTGCGTCCCTCGCAACTCCTCCCAGTTTGTCTCGCCTGCCGCTTCCCTGATACACTCCTCCCCTCCTCCGCCACAATCGCCGCCGCAATCCCCTCCGCAATCGCCGCCGCAATCACCACCGCAAGCACCGCCGCAATCACCGCCGCAGTCACCGCCACAAGCACCGCCACAGTCTCCCCCACCGCAGAAGCAGTCGCCACCTCCTACACCGGTTTCCGACGATGATATCATCGGAAACGTGGAAGGCCCTCGAGCGGAAACCATGTTCGATGATATGATCGAGGCGATGTCGGAGATTCCGGAATTTGTACCAACGACGACGGAATGGAATGGGACGGATCCAATCGATCTCAGCAAGATCTGCAGCAACACGGACAACCCTCAGCTCTGTACCAAATCGATTTCAACTCACCTCAAGCCAGGAACGAACCAAATCGATCCTGTTTCGGCGCTGAAGACCGAAATTGGGGAATCGATTATCAAGGTGCAGAATGCAATGGAAAGAGCGCGCGAACTCCGCAAAGATCCATCGACATCGAAACTCGCAAACGTGTGCTTAGCTACTTGCCTTGACACTTACAATTTGGCGATCTTTGACCTAAAAAAAGTATTGGAATCGATCGAACATCACGACGCTGGTACAATGGAGAGCTACCTCAGCGCCGTACTCTCCAATATCGGGACCTGCGATGATTCTTTTGCAGAGATGGGCTTGGTTTCGCCATTGGATAATCTTGCAATCGAGCTGAACAAGTTCGCAAGCAATTGCTTGGCGATTTCCAAAATGCTTCTGTAA

Coding sequence (CDS)

ATGAAGCTCAATAATGGCGTCATTACTAATTTCTTCCTCTTCCTCTTCCTCGCCCTTTCTTCCTTCTCTGCCAATGCCATTTGCGTCCCTCGCAACTCCTCCCAGTTTGTCTCGCCTGCCGCTTCCCTGATACACTCCTCCCCTCCTCCGCCACAATCGCCGCCGCAATCCCCTCCGCAATCGCCGCCGCAATCACCACCGCAAGCACCGCCGCAATCACCGCCGCAGTCACCGCCACAAGCACCGCCACAGTCTCCCCCACCGCAGAAGCAGTCGCCACCTCCTACACCGGTTTCCGACGATGATATCATCGGAAACGTGGAAGGCCCTCGAGCGGAAACCATGTTCGATGATATGATCGAGGCGATGTCGGAGATTCCGGAATTTGTACCAACGACGACGGAATGGAATGGGACGGATCCAATCGATCTCAGCAAGATCTGCAGCAACACGGACAACCCTCAGCTCTGTACCAAATCGATTTCAACTCACCTCAAGCCAGGAACGAACCAAATCGATCCTGTTTCGGCGCTGAAGACCGAAATTGGGGAATCGATTATCAAGGTGCAGAATGCAATGGAAAGAGCGCGCGAACTCCGCAAAGATCCATCGACATCGAAACTCGCAAACGTGTGCTTAGCTACTTGCCTTGACACTTACAATTTGGCGATCTTTGACCTAAAAAAAGTATTGGAATCGATCGAACATCACGACGCTGGTACAATGGAGAGCTACCTCAGCGCCGTACTCTCCAATATCGGGACCTGCGATGATTCTTTTGCAGAGATGGGCTTGGTTTCGCCATTGGATAATCTTGCAATCGAGCTGAACAAGTTCGCAAGCAATTGCTTGGCGATTTCCAAAATGCTTCTGTAA

Protein sequence

MKLNNGVITNFFLFLFLALSSFSANAICVPRNSSQFVSPAASLIHSSPPPPQSPPQSPPQSPPQSPPQAPPQSPPQSPPQAPPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAETMFDDMIEAMSEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDPVSALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEHHDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL

Homology

[Close]

BLAST of Moc08g34430 vs. NCBI nr
Match: XP_022155317.1 (uncharacterized protein LOC111022449 [Momordica charantia])

HSP 1 Score: 350.5 bits (898), Expect = 1.4e-92
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 115 MFDDMIEAMSEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDP 174
           MFDDMIEAMSEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDP
Sbjct: 1   MFDDMIEAMSEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDP 60

Query: 175 VSALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESI 234
           VSALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESI
Sbjct: 61  VSALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESI 120

Query: 235 EHHDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
           EHHDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL
Sbjct: 121 EHHDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 177

[Close]

BLAST of Moc08g34430 vs. NCBI nr
Match: KAG7036636.1 (hypothetical protein SDJN02_00256, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 164.9 bits (416), Expect = 1.1e-36
Identity = 130/288 (45.14%), Postives = 176/288 (61.11%), Query Frame = 0


Query: 7   VITNFFLFLFLALSS--FSANAICVPRNSSQFVSPAASLIHSSPPPPQSPPQSPPQSPPQ 66
           +I    +FL LALSS  F AN +CVPRNS+ F SP  SLI     P QS    P  S   
Sbjct: 48  IINGLNVFLLLALSSLFFPANTVCVPRNSAPFGSPVDSLIAPQASPSQSSSPQPSVSTSS 107

Query: 67  SPPQAPPQSPPQSPPQA-PPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAETMFDDMIEAM 126
           SP   P QSP  S   A   Q       SP       D    + +  ++E       ++ 
Sbjct: 108 SP---PAQSPTDSEDSALNSQESYSGALSPSDMEFPFDSYSSSEKSEKSEASEASSPQSP 167

Query: 127 SEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDPVSALKTEIG 186
           S  P    ++++ + T P DL +IC  T NPQLCTKSIS+H+    ++++P SALKTEI 
Sbjct: 168 SSTPPTSASSSD-DSTVPTDLEEICDVTSNPQLCTKSISSHI--DESEVNPTSALKTEIE 227

Query: 187 ESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEHHDAGTME 246
           ESI +V+ A+ + + LRK  S SK+   C  TCL+T+++AI+DL+  LESIE  D GTME
Sbjct: 228 ESIKEVRKAVTKLKSLRK--SASKVEISCYDTCLETFDMAIYDLQSGLESIETKDTGTME 287

Query: 247 SYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
           S L+AV+S++ TCDD+F EMG+ SPLDNL+ +++K+ASNCLAISK+LL
Sbjct: 288 SVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 327

[Close]

BLAST of Moc08g34430 vs. NCBI nr
Match: KAG6606934.1 (hypothetical protein SDJN03_00276, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 164.9 bits (416), Expect = 1.1e-36
Identity = 129/288 (44.79%), Postives = 177/288 (61.46%), Query Frame = 0


Query: 7   VITNFFLFLFLALSS--FSANAICVPRNSSQFVSPAASLIHSSPPPPQSPPQSPPQSPPQ 66
           +I    +FL LALSS  F AN +CVPRNS+ F SP  SLI     P QS   S PQ    
Sbjct: 48  IINGLNVFLLLALSSLFFPANTVCVPRNSAPFGSPVDSLIAPQASPSQS---SSPQPSVS 107

Query: 67  SPPQAPPQSPPQSPPQA-PPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAETMFDDMIEAM 126
           + P  P QSP  S   A   Q       SP       D    + +  ++E       ++ 
Sbjct: 108 TSPSPPAQSPTDSEDSALNSQESYSGALSPSDMEFPFDSYSSSEKSEKSEASEASSPQSP 167

Query: 127 SEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDPVSALKTEIG 186
           S  P    ++++ + T P DL +IC  T +PQLCTKSIS+H+    ++++P SALKTEI 
Sbjct: 168 SSTPPTSASSSD-DSTVPTDLEEICDVTSDPQLCTKSISSHI--DESEVNPTSALKTEIE 227

Query: 187 ESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEHHDAGTME 246
           ESI +V+ A+ + + LRK  S SK+   C  TCL+T+++AI+DL+  LESIE  D GTME
Sbjct: 228 ESIKEVRKAVTKLKSLRK--SASKVEISCYDTCLETFDMAIYDLQSGLESIETKDTGTME 287

Query: 247 SYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
           S L+AV+S++ TCDD+F EMG+ SPLDNL+ +++K+ASNCLAISK+LL
Sbjct: 288 SVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 327

[Close]

BLAST of Moc08g34430 vs. NCBI nr
Match: XP_022949037.1 (pectinesterase inhibitor 10 [Cucurbita moschata])

HSP 1 Score: 164.1 bits (414), Expect = 1.8e-36
Identity = 132/291 (45.36%), Postives = 177/291 (60.82%), Query Frame = 0


Query: 7   VITNFFLFLFLALSS--FSANAICVPRNSSQFVSPAASLIHSSPPPPQSPPQSPPQSPPQ 66
           +I    +FL LALSS  F AN +CVPRNS+ F SP  SLI     P  SP QS   S PQ
Sbjct: 48  IINGLNVFLLLALSSLFFPANTVCVPRNSAPFGSPVDSLI----APQASPSQS---SSPQ 107

Query: 67  SPPQAPPQSPPQSPPQAPPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAETMFDDMIEAMS 126
                 P  P QSP  +   +   Q+        SD      +E P       +  EA S
Sbjct: 108 PSVSTSPSPPAQSPTNSEDSALNSQESYSDALSPSD------MEFPFDSYSSSEKSEASS 167

Query: 127 -EIPEFVPTTTEWNGTD---PIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDPVSALKT 186
            + P   P T+  +  D   P DL +IC  T +PQLCTKSIS+H+    ++++P SALKT
Sbjct: 168 PQSPSSTPPTSASSSDDSTVPTDLEEICDVTSDPQLCTKSISSHI--DESEVNPTSALKT 227

Query: 187 EIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEHHDAG 246
           EI ESI +V+ A+ + + LRK  S SK+   C  TCL+T+++AI+DL+  LESI+  D G
Sbjct: 228 EIEESIKEVRKAVTKLKSLRK--SASKVEISCYDTCLETFDMAIYDLQSGLESIDTKDTG 287

Query: 247 TMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
           TMES L+AV+S++ TCDD+F EMG+ SPLDNL+ +++K+ASNCLAISK+LL
Sbjct: 288 TMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 321

[Close]

BLAST of Moc08g34430 vs. NCBI nr
Match: XP_022998626.1 (pectinesterase inhibitor 10 [Cucurbita maxima])

HSP 1 Score: 162.5 bits (410), Expect = 5.3e-36
Identity = 130/295 (44.07%), Postives = 180/295 (61.02%), Query Frame = 0


Query: 1   MKLNNGVITNFFLFLFLALSSFSANAICVPRNSSQFVSPAASLIHSSPPPPQSPPQSPPQ 60
           M+L NG+  N FL L L+   F AN +CVPRNS    +P  SL+ S   P  SP QS   
Sbjct: 46  MELINGL--NVFLLLALSSLFFPANTVCVPRNS----APFGSLVDSFVAPQASPSQS--- 105

Query: 61  SPPQSPPQAPPQSPPQSPPQAPPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAETMFDDMI 120
           S PQ      P  P QSP  +   +   Q+        SD +   +      ++   +  
Sbjct: 106 SSPQPSISISPSPPAQSPTDSEDSALNSQESDSDALSPSDMEFPFDSYSSSEKSEASEAS 165

Query: 121 EAMS-EIPEFVPTTTEWNGTD---PIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDPVS 180
           EA S + P   P T+  + +D   P DL +IC  T +PQLCTKSIS+H+    ++++P S
Sbjct: 166 EASSPQSPSSTPPTSASSSSDSTVPTDLEEICDVTSDPQLCTKSISSHI--DESEVNPTS 225

Query: 181 ALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEH 240
           ALKTEI ESI +V+ A+ + + LRK  S SK+   C  TCL+T+++AIFDL+  LESI+ 
Sbjct: 226 ALKTEIEESIKEVRKAVTKLKLLRK--SASKVEIACYDTCLETFDMAIFDLQSGLESIDT 285

Query: 241 HDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
            D GTMES L+AV+S++ TCDD+F EMG+ SPLDNL+ +++K+ASNCLAISK+LL
Sbjct: 286 KDTGTMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 327

[Close]

BLAST of Moc08g34430 vs. ExPASy TrEMBL
Match: A0A6J1DP11 (uncharacterized protein LOC111022449 OS=Momordica charantia OX=3673 GN=LOC111022449 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 6.7e-93
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 115 MFDDMIEAMSEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDP 174
           MFDDMIEAMSEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDP
Sbjct: 1   MFDDMIEAMSEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDP 60

Query: 175 VSALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESI 234
           VSALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESI
Sbjct: 61  VSALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESI 120

Query: 235 EHHDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
           EHHDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL
Sbjct: 121 EHHDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 177

[Close]

BLAST of Moc08g34430 vs. ExPASy TrEMBL
Match: A0A6J1GAW7 (pectinesterase inhibitor 10 OS=Cucurbita moschata OX=3662 GN=LOC111452501 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 8.9e-37
Identity = 132/291 (45.36%), Postives = 177/291 (60.82%), Query Frame = 0


Query: 7   VITNFFLFLFLALSS--FSANAICVPRNSSQFVSPAASLIHSSPPPPQSPPQSPPQSPPQ 66
           +I    +FL LALSS  F AN +CVPRNS+ F SP  SLI     P  SP QS   S PQ
Sbjct: 48  IINGLNVFLLLALSSLFFPANTVCVPRNSAPFGSPVDSLI----APQASPSQS---SSPQ 107

Query: 67  SPPQAPPQSPPQSPPQAPPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAETMFDDMIEAMS 126
                 P  P QSP  +   +   Q+        SD      +E P       +  EA S
Sbjct: 108 PSVSTSPSPPAQSPTNSEDSALNSQESYSDALSPSD------MEFPFDSYSSSEKSEASS 167

Query: 127 -EIPEFVPTTTEWNGTD---PIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDPVSALKT 186
            + P   P T+  +  D   P DL +IC  T +PQLCTKSIS+H+    ++++P SALKT
Sbjct: 168 PQSPSSTPPTSASSSDDSTVPTDLEEICDVTSDPQLCTKSISSHI--DESEVNPTSALKT 227

Query: 187 EIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEHHDAG 246
           EI ESI +V+ A+ + + LRK  S SK+   C  TCL+T+++AI+DL+  LESI+  D G
Sbjct: 228 EIEESIKEVRKAVTKLKSLRK--SASKVEISCYDTCLETFDMAIYDLQSGLESIDTKDTG 287

Query: 247 TMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
           TMES L+AV+S++ TCDD+F EMG+ SPLDNL+ +++K+ASNCLAISK+LL
Sbjct: 288 TMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 321

[Close]

BLAST of Moc08g34430 vs. ExPASy TrEMBL
Match: A0A6J1KAN1 (pectinesterase inhibitor 10 OS=Cucurbita maxima OX=3661 GN=LOC111493211 PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 2.6e-36
Identity = 130/295 (44.07%), Postives = 180/295 (61.02%), Query Frame = 0


Query: 1   MKLNNGVITNFFLFLFLALSSFSANAICVPRNSSQFVSPAASLIHSSPPPPQSPPQSPPQ 60
           M+L NG+  N FL L L+   F AN +CVPRNS    +P  SL+ S   P  SP QS   
Sbjct: 46  MELINGL--NVFLLLALSSLFFPANTVCVPRNS----APFGSLVDSFVAPQASPSQS--- 105

Query: 61  SPPQSPPQAPPQSPPQSPPQAPPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAETMFDDMI 120
           S PQ      P  P QSP  +   +   Q+        SD +   +      ++   +  
Sbjct: 106 SSPQPSISISPSPPAQSPTDSEDSALNSQESDSDALSPSDMEFPFDSYSSSEKSEASEAS 165

Query: 121 EAMS-EIPEFVPTTTEWNGTD---PIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDPVS 180
           EA S + P   P T+  + +D   P DL +IC  T +PQLCTKSIS+H+    ++++P S
Sbjct: 166 EASSPQSPSSTPPTSASSSSDSTVPTDLEEICDVTSDPQLCTKSISSHI--DESEVNPTS 225

Query: 181 ALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEH 240
           ALKTEI ESI +V+ A+ + + LRK  S SK+   C  TCL+T+++AIFDL+  LESI+ 
Sbjct: 226 ALKTEIEESIKEVRKAVTKLKLLRK--SASKVEIACYDTCLETFDMAIFDLQSGLESIDT 285

Query: 241 HDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
            D GTMES L+AV+S++ TCDD+F EMG+ SPLDNL+ +++K+ASNCLAISK+LL
Sbjct: 286 KDTGTMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 327

[Close]

BLAST of Moc08g34430 vs. ExPASy TrEMBL
Match: A0A0A0KWP8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 3.9e-32
Identity = 123/291 (42.27%), Postives = 169/291 (58.08%), Query Frame = 0


Query: 3   LNNGVITNFFLFLFLALSSFSANAICVPRNSSQFVSPAASLIHSSPPPPQSPPQSPPQSP 62
           +NN +   F L  F ++ SF A A+CVPRN+S   S A   +      PQ  PQ  PQSP
Sbjct: 4   INNSIHNVFLLLAFSSVFSF-AVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQSP 63

Query: 63  PQSPPQAPPQ--SPPQSPPQAPPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAETMFDDMI 122
           P       P+  SP  SP Q    S   + +S      +++D +G + G   +    D+I
Sbjct: 64  PAVTVSEEPEAASPETSPSQPSSASESEEAESEEAASETEEDDLGLLGGGLDQLHSADLI 123

Query: 123 EAMSEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDPVSALKT 182
              S +   V T+          L  IC  T NPQLC  SIS+H++ GT ++DP SALKT
Sbjct: 124 ---SSVTSSVTTS----------LVDICDVTSNPQLCKTSISSHIE-GT-KVDPASALKT 183

Query: 183 EIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEHHDAG 242
           EI ESI +V  A+     LRKD + S+    C  TCL+ + +AI DLK  +ESI   DAG
Sbjct: 184 EIDESIKEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAG 243

Query: 243 TMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
            MES L+AV++++ TCDD+FAEMG+ SPLD+L+ +++K+ASNCLAISK+LL
Sbjct: 244 RMESVLTAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 278

[Close]

BLAST of Moc08g34430 vs. ExPASy TrEMBL
Match: A0A5A7TT72 (Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001500 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 4.0e-29
Identity = 122/291 (41.92%), Postives = 166/291 (57.04%), Query Frame = 0


Query: 1   MKLNNGVITNFFLFLFLALSSFSANAICVPRNSSQFVSPAASLIHSSPPPPQSPPQSPPQ 60
           M+L N  I N FL L  +   FSA AICVP N +        +  ++ PP      SP  
Sbjct: 1   MELINNNINNVFLLLAFSSVFFSAIAICVPLNIN-----ITDVESTAIPPLLEQTVSPQV 60

Query: 61  SPPQSPPQAPPQSPPQSPPQAPPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAETMFDDMI 120
           +P QSPP     S P  P    PQ+    + S     +SD++  G +      +  D +I
Sbjct: 61  APDQSPPAVTTVSQP--PEATSPQTSTSDQSS--SNEISDEENSGLLGLDYLHS--DGLI 120

Query: 121 EAMSEIPEFVPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTNQIDPVSALKT 180
              +              T   +L +IC  T NPQLC  SIS+H++ GT ++DP SALKT
Sbjct: 121 STAA-------------STVKTNLVEICDVTSNPQLCKTSISSHIE-GT-KVDPASALKT 180

Query: 181 EIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEHHDAG 240
           EI ESI +V  A+     LRKD S S+    C  TCL+ +++AI DLK  +ESI+  DAG
Sbjct: 181 EIEESIKEVTKAIATLNSLRKDSSASETEIACYDTCLENFDMAIEDLKAGVESIDARDAG 240

Query: 241 TMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNKFASNCLAISKMLL 292
            MES L+AVL+++ TCDD+FAEMG+ SPLDNL+ +++K+ASNCLAISK+LL
Sbjct: 241 RMESVLTAVLTDLTTCDDTFAEMGVDSPLDNLSTKMSKYASNCLAISKLLL 265

[Close]

BLAST of Moc08g34430 vs. TAIR 10
Match: AT1G10770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-11
Identity = 43/144 (29.86%), Postives = 69/144 (47.92%), Query Frame = 0


Query: 147 ICSNTDNPQLCTKSISTHLKPGTNQIDPVSALKTEIGESIIKVQNAMERARELRKDPSTS 206
           ICS+T  P LC   +     P       + AL+ +   ++       E AR         
Sbjct: 37  ICSHTAYPSLCRPLVKRVTSPRKATHRTIQALEAKTKLAL------AETAR--------F 96

Query: 207 KLANVCLATCLDTYNLAIFDLKKVLESIEHHDAGTMESYLSAVLSNIGTCDDSFAEMGLV 266
           K  N  ++TC +T   A+++L    +SI   D   M +YL+A +S+ G C D F E   V
Sbjct: 97  KNGNQAVSTCYETLGDAVYNLASARKSIRKRDVPAMNTYLTAAVSDYGACVDGFIETQQV 156

Query: 267 SPLDNLAIELNKFASNCLAISKML 291
           + + N  ++L K +SNCLA+S ++
Sbjct: 157 NAIQNAVVDLRKISSNCLALSTLI 166

[Close]

BLAST of Moc08g34430 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 4.5e-09
Identity = 66/242 (27.27%), Postives = 108/242 (44.63%), Query Frame = 0


Query: 80  QAPPQSPPPQKQSPP-----------PTPVSDDDIIGNVEGPRA--------ETMFDDMI 139
           Q P  SP P    PP           P+P S+D   G+   P A        +   D   
Sbjct: 37  QVPASSPDPSLIEPPVSSISVVPASAPSPTSEDINTGSNLAPTAAVSAPGPSQEDIDINF 96

Query: 140 EAMSEIPEFVPTTTEWNGTD----PIDLS--KICSNTDNPQLCTKSISTHLKP------G 199
           ++++ I +  P     N  D     ID +   +C NTD    C  +I   L+       G
Sbjct: 97  DSVTNIADLAPKFEHINKFDFSSMKIDTTAKDLCKNTDYNNECIAAILPDLQKQAEGEGG 156

Query: 200 TNQIDPVSALKTEIGESIIKVQNA-MERARELRKDPSTSKLANVCLATCLDTYNLAIFDL 259
              I P   ++ E  ES++K  NA ++ A+ + +D  T       ++ C++ Y+  I  L
Sbjct: 157 DGGIAPKDVMRME-AESLLKKANATLDYAKRVVEDRKTPMPVKEAMSVCVENYDSLISGL 216

Query: 260 KKVLESIEHHDAGTMESYLSAVLSNIGTCDDSFAEMGLV-SPLDNLAIELNKFASNCLAI 289
           +    ++E  D G ++S LSA +S++ TC D+F ++  V SP  +L   + K  SN LA+
Sbjct: 217 EDAKMAMEEGDYGRLDSVLSAAISDVSTCSDNFVDIPDVDSPTASLDDLMKKLCSNVLAM 276

[Close]

BLAST of Moc08g34430 vs. TAIR 10
Match: AT1G02550.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.0e-08
Identity = 63/223 (28.25%), Postives = 97/223 (43.50%), Query Frame = 0


Query: 73  SPPQSPPQAPPQSPPPQKQSPPPTPVSDDDIIGNVEGPRAE---TMFDDMIEAMSEIPEF 132
           S P S  +      P    SP   P +D D+  N      +       D+      +P+F
Sbjct: 19  SSPSSSTRIISSIVPSAAPSPAVAPTTDGDVDENDFSAFTQWNILNLTDLKSTFKNLPDF 78

Query: 133 VPTTTEWNGTDPIDLSKICSNTDNPQLCTKSISTHLKPGTN-QIDPVSALKTEIGESIIK 192
                      P  +  +CSNTD    C  SI   L+     +  P+  L+ E+     K
Sbjct: 79  SKLNISSLHVSPA-VGSVCSNTDYAAECIVSILPLLRDFRKFEPKPIDVLRMEMSALYEK 138

Query: 193 VQNAMERARELRKDPSTSKLANVCLATCLDTYNLAIFDLKKVLESIEHHDAGTMESYLSA 252
               ++ A+ L  D ST +     L  C+D Y   + DLK    +++  D   +ES +SA
Sbjct: 139 ANATLDLAKRLIVDKSTPRDVADVLDLCVDNYESLLDDLKDASVAVDDGDFERLESVVSA 198

Query: 253 VLSNIGTCDDSFAEMG-LVSPLDNLAIELNKFASNCLAISKML 291
            ++++ TC D+FAE   L SP+ N+   L K  SN LAIS+M+
Sbjct: 199 AIADVVTCSDAFAESSELESPMANVDDFLKKLCSNVLAISQMI 240

[Close]

BLAST of Moc08g34430 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.0e-05
Identity = 75/252 (29.76%), Postives = 113/252 (44.84%), Query Frame = 0


Query: 47  SPPPPQSPPQSPPQSPPQSPPQAPPQSPPQSPPQAPPQSPPPQKQSPPPTPVSDDDIIGN 106
           S P P      P  SP  SP +    SP  S P+AP  S  P+    PP  +   DI   
Sbjct: 34  STPAPAPEVMKPIASPANSPAEIYTDSPVAS-PEAPADSFSPKN---PPVEI---DIYSP 93

Query: 107 VEGPRAETMFDDM-IEAMSEIPEFVPTTTEWNG--TDPI---DLSKICSNTDNPQLCTKS 166
              P A     +   E+ S  P  +  ++        P+   ++  IC  TDNP LC  S
Sbjct: 94  AASPEAPVASPEAPAESSSAAPSGIKVSSSPKSLLNPPLLSPEIKTICGKTDNPPLCESS 153

Query: 167 ISTHLKPGTNQIDP--VSALKTEIGESIIKVQNAMERARELRKDPSTSKLANVCLATCLD 226
           +S  L P   Q+ P   S L   I  SI   + AM            + +  V  + C +
Sbjct: 154 VSPLLTP---QLKPNTSSVLILAIQASITATKAAM------------AIVEKVDASDCQE 213

Query: 227 TYNLAIFDLKKVLESIEHHDAGTMESYLSAVLSNIGTCDDSFAEMGLVSPLDNLAIELNK 286
            Y+ A+ +L+  + +++  D  T+ + LSA +++  TC+D F E G  +PL  +A +L K
Sbjct: 214 LYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPLAYVADKLTK 263

Query: 287 FASNCLAISKML 291
             SNCLAIS ++
Sbjct: 274 MVSNCLAISTLI 263

The following BLAST results are available for this feature:

BLAST of Moc08g34430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155317.1	1.4e-92	100.00	uncharacterized protein LOC111022449 [Momordica charantia]	[more]
KAG7036636.1	1.1e-36	45.14	hypothetical protein SDJN02_00256, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6606934.1	1.1e-36	44.79	hypothetical protein SDJN03_00276, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022949037.1	1.8e-36	45.36	pectinesterase inhibitor 10 [Cucurbita moschata]	[more]
XP_022998626.1	5.3e-36	44.07	pectinesterase inhibitor 10 [Cucurbita maxima]	[more]

BLAST of Moc08g34430 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc08g34430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DP11	6.7e-93	100.00	uncharacterized protein LOC111022449 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1GAW7	8.9e-37	45.36	pectinesterase inhibitor 10 OS=Cucurbita moschata OX=3662 GN=LOC111452501 PE=4 S...	[more]
A0A6J1KAN1	2.6e-36	44.07	pectinesterase inhibitor 10 OS=Cucurbita maxima OX=3661 GN=LOC111493211 PE=4 SV=...	[more]
A0A0A0KWP8	3.9e-32	42.27	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 S...	[more]
A0A5A7TT72	4.0e-29	41.92	Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Moc08g34430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G10770.1	1.7e-11	29.86	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47340.1	4.5e-09	27.27	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G02550.1	1.0e-08	28.25	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G36659.1	1.0e-05	29.76	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 139..286 e-value: 4.0E-28 score: 109.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 145..286 e-value: 1.0E-20 score: 74.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 144..290 e-value: 3.1E-23 score: 80.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 144..290 e-value: 2.9E-27 score: 97.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 145..290
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 44..95
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 41..108
None	No IPR available	PANTHER	PTHR31080:SF68	PECTINESTERASE INHIBITOR-LIKE	coord: 125..289
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 125..289
None	No IPR available	CDD	cd15800	PMEI-like_2	coord: 143..290 e-value: 1.01966E-49 score: 159.833

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g34430.1	Moc08g34430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity