Moc08g10020 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g10020
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	phosphatidylinositol-glycan biosynthesis class F protein
Location	chr8: 7404977 .. 7407911 (+)
RNA-Seq Expression	Moc08g10020
Synteny	Moc08g10020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACGGAGAAGGAGATGGCCAGTAAAGCCCCGACTGCCGTATCGATTGCGGAAGCCTTCCTCGTCCACCTGATCTGCGGCTTGGGGCTAGCCCTAGCTCTCTGGATTGCTCGATACATCTACTCCACCGATCTCATCTCCAATCCTTCTCATACTCTCTTCTTGATTTGGGTATTTTTTCTCTCAATTCTCTTCTTGATTTCGTTTCCTAATTTACTCCCTGCATTTTGAAAGATTTTTCTTCTTTCTCTCTGTTCAGGCTCTTGAGTGTCCGATTGTCATCCTTCTTTACAGCCGCTACCGTCTGGACCGAGAACAATGCTCGGTAAGGTTGACTCTCTCTTGTTTCACGATAAAGTGTATCGGTTGTTCTGAAATCAGATAAGAAATTTTGAAAAACTTGGACCATCTTCTGTGCGAATTTGCAGTACCTGAAAGCTGTTACGAGAGGCATACTTGGACTCCCTGCTGGTTAGTTGAAGAAATCTCATTACCATTTGGTACTTCTTTCGATTTTCTTTTGTTTAATTAGCTATGAGTGCTTTTGAAGTTGCGCTTCATCTGTCTTTCCAATTGAGCATTCTGAGGATCGAAATATTGTATGCGAAAATCGAAGACGTGTTAAATTCAATAACCATTTCTCGAAATGGATATTGCTCAAATTTTAGGTGAAATGAGATTTCAAATTTGGTGATGTGAGAGTATAGGTATTTTTGTTCCCATGTGCGTCCACGATCACTCTCTGGAGACTACCACGCATTTGTTTTTCTTATTCATAATGATTTGTATTTGTTTGGTTCTTCTCGCTCAACTTGTCATAATGGGATCAATGTGGCCTCTATTATGCAGGTGCTGTTATTAATGCTTTTGGAGCAATAGTTCTAGGCGCTCCCGTTGGTGCTCAGTACGTGAATAAGTGACTGAAAAGCTATATTTTCATCTCCCATGGATTCCAAATTTTGAATCAATTTTTATATTTGATTGGAAAACTTATACCAGAATTTGTACAACAAATAAGGCAAGATCTGCTCCCAGCATCAGCTGGCATTTTATTTTATCACCATTATGGGAACATTACTTTGGTTTTGGTCTTCCTTTTCCAAAATAAAGCTTAATCCAAATTGTGAGTATTTCAAGTGGAATTTTACTTGGGAAGATTAATCGACAGTATCATCAGTCATGATTTGCTGTTTTATAATTATGGATATTGATGTTCCACAGATTTAAGCTTTAAGGCTAAATCACGATAATATTAATACATTTGATACTGAAGTGTTTTTGTGTACATGGTCTGAATACTTCTTCTTGGCACAGATACTTCTCGAAGACCCTTAACTGGTCACTTGTGATGTCATTGTTCAATGTGAGTAAGCTATTCTTAAGATATTTCCTTTGATGACTATATCTCTAACTATATTGTTTCAATTTCTTCCACCTTTTTGAATCCTGTTATATAGTCATTGGCTGTTGTTTTACATCTTTGTGAGATGGATAATGGGACCTATATTTTCTTTTTTGGTTTTCTCAGGTTATGGTTTTAACCCTTTAAATTAGTTAATGACTATTATGAGATTCATGGAAGTGGAGCTTTCTCATATCTGGATATTTTTTCAGTTTGTTTTGAGGATTATTATCATCGTATTTACTTTCATGGTAAATGTTGTGCTGTGTTAGAAACCCTTCCCCTTCTCGTATGGATATGTTAGTCCTTTATTGATAGTGGTTTTTGCTCGTCTTGTAGATCGTGCCTTCAGCATGTGTCTTTGGTTCATCATGGATGGACTGGCAGCGCCTATTTGCTTACACAAAGTAAGGCTCCTGATGTGTTATTTATCTCTCTTTACTTGACGTGGATGCATTCTTCTACTAGCAAAATATTTAGTTTTTAACAAAGGTAAATACAAACTCAGAATAAAATGTTTATGTAAAGGTAGTCGATGCTGTGCAATTGCAGGCCCAATGGAACTATTGATCATATGATATGCATTCCAGCACATGGGGCCATTATTGGAGCCTGGTTTGGCGCATGGCCCATGCCACTTGATTGGGAAAGGCCTTGGCAGGTACTATTTTTCCATACTGCACTTGTGTTTTTGCCTATAAAGCTCGGCCAACAAACCTCATTGACATTAAATAGTGTCATTTGATAATCTCTTGTTTCAAAGTTGATGAACTATAAAATGGTACGTCTGGGCATCCCAAAAAAAATAATGAGTCAAGTTTACTTACCTCTAAAATGTGATCTAAGCCGATAAAAAGTTATATGATAGAGATTAGTTTTTACTGCATCTATTCGTGACTTCTAGGCATTCAAAGACAAAATAATGAGTAGAGTCCACTTATCTATAACTTGTGAACTTAGCTGATACATACGATAGTACTCAATTCTTTTCGGAACTTATTCATAAATGACAATTCACTACATTTTTTCATGTGACCATGCTGTTATATTGATTTGAATCAGATTTTGGGATATGGTATCAATTTATGTCTATTTGCTACCCATATAGTTCCTTTGACAATTCCATACTTAATGGTTGTCGCCTCTTGCTCTAGTGCTCCTAATCTCGATGGTCTTGATGTGGCATTAGATTAGGTAAATTATGTTTTGAATAAATCCTAAGACCAACTAGAATGCTCTCCTATTACAAACAATAAAGAAACTAACAGAGTTAGATAGGAGTGACATCAAATAAACCAAAGTCCCTTGTGTGTGTGAAAATGTTAAACCAATCATGTAAATTCAGGTTATTTTTCTTAGTTCTCTTGCATTGCATCTATGCGAACTCTATTAAATAAAGATCACTTTAATATGCAGGAGTGGCCAATATGTGTGAGTTACGGGGCAGTAATTGGGTACTCGACCTCAATGGTAGCATCCTTGGCTTTTTCACGTGTTCGAAGTGGCTTGCAGCACGTTAAAGGAGACTAA

mRNA sequence

ATGGCGACGGAGAAGGAGATGGCCAGTAAAGCCCCGACTGCCGTATCGATTGCGGAAGCCTTCCTCGTCCACCTGATCTGCGGCTTGGGGCTAGCCCTAGCTCTCTGGATTGCTCGATACATCTACTCCACCGATCTCATCTCCAATCCTTCTCATACTCTCTTCTTGATTTGGGCTCTTGAGTGTCCGATTGTCATCCTTCTTTACAGCCGCTACCGTCTGGACCGAGAACAATGCTCGTACCTGAAAGCTGTTACGAGAGGCATACTTGGACTCCCTGCTGGTGCTGTTATTAATGCTTTTGGAGCAATAGTTCTAGGCGCTCCCGTTGGTGCTCAATACTTCTCGAAGACCCTTAACTGGTCACTTGTGATGTCATTGTTCAATATCGTGCCTTCAGCATGTGTCTTTGGTTCATCATGGATGGACTGGCAGCGCCTATTTGCTTACACAAAGCCCAATGGAACTATTGATCATATGATATGCATTCCAGCACATGGGGCCATTATTGGAGCCTGGTTTGGCGCATGGCCCATGCCACTTGATTGGGAAAGGCCTTGGCAGGAGTGGCCAATATGTGTGAGTTACGGGGCAGTAATTGGGTACTCGACCTCAATGGTAGCATCCTTGGCTTTTTCACGTGTTCGAAGTGGCTTGCAGCACGTTAAAGGAGACTAA

Coding sequence (CDS)

ATGGCGACGGAGAAGGAGATGGCCAGTAAAGCCCCGACTGCCGTATCGATTGCGGAAGCCTTCCTCGTCCACCTGATCTGCGGCTTGGGGCTAGCCCTAGCTCTCTGGATTGCTCGATACATCTACTCCACCGATCTCATCTCCAATCCTTCTCATACTCTCTTCTTGATTTGGGCTCTTGAGTGTCCGATTGTCATCCTTCTTTACAGCCGCTACCGTCTGGACCGAGAACAATGCTCGTACCTGAAAGCTGTTACGAGAGGCATACTTGGACTCCCTGCTGGTGCTGTTATTAATGCTTTTGGAGCAATAGTTCTAGGCGCTCCCGTTGGTGCTCAATACTTCTCGAAGACCCTTAACTGGTCACTTGTGATGTCATTGTTCAATATCGTGCCTTCAGCATGTGTCTTTGGTTCATCATGGATGGACTGGCAGCGCCTATTTGCTTACACAAAGCCCAATGGAACTATTGATCATATGATATGCATTCCAGCACATGGGGCCATTATTGGAGCCTGGTTTGGCGCATGGCCCATGCCACTTGATTGGGAAAGGCCTTGGCAGGAGTGGCCAATATGTGTGAGTTACGGGGCAGTAATTGGGTACTCGACCTCAATGGTAGCATCCTTGGCTTTTTCACGTGTTCGAAGTGGCTTGCAGCACGTTAAAGGAGACTAA

Protein sequence

MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD

Homology

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BLAST of Moc08g10020 vs. NCBI nr
Match: XP_022153416.1 (phosphatidylinositol-glycan biosynthesis class F protein [Momordica charantia])

HSP 1 Score: 465.7 bits (1197), Expect = 2.3e-127
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL 60
           MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL
Sbjct: 1   MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL 60

Query: 61  ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN 120
           ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN
Sbjct: 61  ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN 120

Query: 121 WSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP 180
           WSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP
Sbjct: 121 WSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP 180

Query: 181 LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD
Sbjct: 181 LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 225

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BLAST of Moc08g10020 vs. NCBI nr
Match: XP_022949007.1 (phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita moschata])

HSP 1 Score: 399.4 bits (1025), Expect = 2.0e-107
Identity = 195/226 (86.28%), Postives = 205/226 (90.71%), Query Frame = 0


Query: 1   MATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWA 60
           M TE KEMASKA  +VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI  
Sbjct: 1   METEKKEMASKASPSVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 61  LECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTL 120
           +ECPIVILLYSRYR DREQCSYLKAV RGILGL AGA+INAFGAIVLGAP+GAQY  KTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAVARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMS F++VPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFSVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           PLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVK D
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

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BLAST of Moc08g10020 vs. NCBI nr
Match: XP_038904140.1 (phosphatidylinositol-glycan biosynthesis class F protein [Benincasa hispida])

HSP 1 Score: 398.3 bits (1022), Expect = 4.5e-107
Identity = 190/222 (85.59%), Postives = 203/222 (91.44%), Query Frame = 0


Query: 4   EKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECP 63
           +KEMA +A   +SI+EAF +HLIC LGLALA WIARYIYSTDLIS+PS TLFLI A+ECP
Sbjct: 5   KKEMACEASIPISISEAFFLHLICALGLALAFWIARYIYSTDLISDPSRTLFLILAIECP 64

Query: 64  IVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSL 123
           IVILLYSRYR DR+QCSYLKAV RG+LGLPAGAVINAFGAIVLGAPVGAQYF KTLNWSL
Sbjct: 65  IVILLYSRYRTDRQQCSYLKAVARGLLGLPAGAVINAFGAIVLGAPVGAQYFVKTLNWSL 124

Query: 124 VMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW 183
           VMSLFNIVPSACVFGSSW DWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW
Sbjct: 125 VMSLFNIVPSACVFGSSWRDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184

Query: 184 ERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           ERPWQEWPICVSYGA++GYS +MVASL FS +R G QHVK D
Sbjct: 185 ERPWQEWPICVSYGAILGYSIAMVASLVFSHLR-GSQHVKRD 225

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BLAST of Moc08g10020 vs. NCBI nr
Match: XP_022998495.1 (phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita maxima])

HSP 1 Score: 395.6 bits (1015), Expect = 2.9e-106
Identity = 193/226 (85.40%), Postives = 202/226 (89.38%), Query Frame = 0


Query: 1   MATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWA 60
           M TE KEM SKA   VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI  
Sbjct: 1   METEKKEMTSKASPPVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 61  LECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTL 120
           +ECPIVILLYSRYR DREQCSYLKA+ RGILGL AGA+INAFGAIVLGAP+GAQY  KTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAMARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMS F +VPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFGVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           PLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVK D
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

[Close]

BLAST of Moc08g10020 vs. NCBI nr
Match: XP_023524788.1 (phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 395.6 bits (1015), Expect = 2.9e-106
Identity = 193/226 (85.40%), Postives = 203/226 (89.82%), Query Frame = 0


Query: 1   MATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWA 60
           M TE KEM SKA   VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI  
Sbjct: 1   METEKKEMTSKASPPVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 61  LECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTL 120
           +ECPIVILLYSRYR DREQCSYLKAV RGILGL AGA+INAFGAIVLGAP+GAQY  KTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAVARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMS F++VPSACVFGSSWMDWQRLFAYT+PNGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFSVVPSACVFGSSWMDWQRLFAYTEPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           PLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVK D
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

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BLAST of Moc08g10020 vs. ExPASy Swiss-Prot
Match: Q9Y7P2 (Uncharacterized protein C1450.15 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC1450.15 PE=3 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 1.3e-16
Identity = 58/171 (33.92%), Postives = 86/171 (50.29%), Query Frame = 0


Query: 40  YIYSTDLISNPSHTL---FLIWALECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGA 99
           Y+    LI NP   L   F IW +   + I + S     R   +  K +  G   +  G+
Sbjct: 326 YLSLNCLIENPYRMLRNTFPIWFIMQTLQIYIQS----PRPPLT-PKRLLAGAASMLIGS 385

Query: 100 VINAFGAIVLGAPVGAQYFSKTLNWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGT 159
           ++ +F  +  GAP+    F  T   +L +S+F + P A     +   WQR F   K    
Sbjct: 386 LLISFILVAFGAPL-LHDFHLTYFCALTLSVFTVYPLASTLAFNTEQWQR-FLTLKSFNV 445

Query: 160 IDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMV 208
           I  M  + + G IIGAWFGA+P+PLDW+RPWQ WPI +  GA +GY+ + +
Sbjct: 446 IGSM-QLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLGYAFAAI 488

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BLAST of Moc08g10020 vs. ExPASy Swiss-Prot
Match: O09101 (Phosphatidylinositol-glycan biosynthesis class F protein OS=Mus musculus OX=10090 GN=Pigf PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.2e-16
Identity = 39/106 (36.79%), Postives = 60/106 (56.60%), Query Frame = 0


Query: 104 IVLGAPVGAQYFSKTLNWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICI 163
           ++ GAP+  +   +T  +++V+S F  VP  C+ G +   W R+F+        ++ + I
Sbjct: 99  VLYGAPL-IELVLETFLFAVVLSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQI 158

Query: 164 PAHGAIIGAWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMVAS 210
               +  GAW GA+P+PLDWERPWQ WPI  + GA  GY   +V S
Sbjct: 159 TTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFGYVAGLVIS 203

[Close]

BLAST of Moc08g10020 vs. ExPASy Swiss-Prot
Match: Q07326 (Phosphatidylinositol-glycan biosynthesis class F protein OS=Homo sapiens OX=9606 GN=PIGF PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-15
Identity = 53/203 (26.11%), Postives = 95/203 (46.80%), Query Frame = 0


Query: 17  IAEAFLVHLICGLGLALALWI-ARYIYSTDLISNPSHTLFLIWALECPIVILLY------ 76
           I      HL+C   + L+++I + ++ +  ++      L +       + ++LY      
Sbjct: 6   IKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYLVVKPN 65

Query: 77  ---SRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSLVMS 136
               R  L  +   +LK     ++   +  VI     ++ GAP+  +   +T  +++++S
Sbjct: 66  TSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVI----FVLYGAPL-IELALETFLFAVILS 125

Query: 137 LFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDWERP 196
            F  VP  C+ G +   W R+F+        ++ + I    + +GAW GA P+PLDWERP
Sbjct: 126 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP 185

Query: 197 WQEWPICVSYGAVIGYSTSMVAS 210
           WQ WPI  + GA  GY   +V S
Sbjct: 186 WQVWPISCTLGATFGYVAGLVIS 203

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BLAST of Moc08g10020 vs. ExPASy Swiss-Prot
Match: Q6BHK4 (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=GPI11 PE=3 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 9.6e-12
Identity = 40/113 (35.40%), Postives = 61/113 (53.98%), Query Frame = 0


Query: 104 IVLGAPVGAQYFSKTLNWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTID----H 163
           I+LGAP+ A +  +T   S+ +SL    PS  ++     D++ L  +   +G  +    +
Sbjct: 127 ILLGAPL-ASHLKETYLLSIHLSLLIFYPSLVLYK---YDYKSLVKFLDADGVYNAIAKN 186

Query: 164 MICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMVASLAF 213
            I + A  A+IG WFG  P+PLDW+R WQ+WPI +  GA IG     +A   F
Sbjct: 187 QILLSAVLAVIGTWFGVIPIPLDWDRDWQQWPITLLTGAYIGSFVGGIACFLF 235

[Close]

BLAST of Moc08g10020 vs. ExPASy Swiss-Prot
Match: Q6C741 (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=GPI11 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-11
Identity = 56/226 (24.78%), Postives = 97/226 (42.92%), Query Frame = 0


Query: 1   MATEKEMASKA--PTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHT----- 60
           MAT K   +KA  P  V    + ++ ++ G    L L   R+I+S  +  +P+       
Sbjct: 1   MATRKAAKTKANGPAHVPSGPSQVLAVVYG---TLLLVYLRFIFSAGITHDPAKVMTQAL 60

Query: 61  --LFLIWALECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVG 120
             L L+    C +V+      ++  +  + + A    +        +  FG +VL     
Sbjct: 61  PGLLLLHMGYCVVVLGNKPGRKIGTDVSTAMIAAALSVF-----FSVIIFGLLVLFGAPA 120

Query: 121 AQYFSKTLNWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIG 180
                 T   ++ MS+  ++P    +      W  + A  +P   +DH+        +IG
Sbjct: 121 ISLVHNTFVCAMHMSILAVLPLFFTYHLDSKVWADIIAMRRP---LDHVYAASV-CTLIG 180

Query: 181 AWFGAWPMPLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRS 218
           AW GA P+P DW+RPWQ+WPI +  GA +GY    +  +A    +S
Sbjct: 181 AWLGAIPIPYDWDRPWQQWPITILAGAYLGYFVGTLGGIALELTKS 214

[Close]

BLAST of Moc08g10020 vs. ExPASy TrEMBL
Match: A0A6J1DKK8 (phosphatidylinositol-glycan biosynthesis class F protein OS=Momordica charantia OX=3673 GN=LOC111020928 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-127
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL 60
           MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL
Sbjct: 1   MATEKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWAL 60

Query: 61  ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN 120
           ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN
Sbjct: 61  ECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLN 120

Query: 121 WSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP 180
           WSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP
Sbjct: 121 WSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMP 180

Query: 181 LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD
Sbjct: 181 LDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 225

[Close]

BLAST of Moc08g10020 vs. ExPASy TrEMBL
Match: A0A6J1GAT8 (phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita moschata OX=3662 GN=LOC111452477 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 9.7e-108
Identity = 195/226 (86.28%), Postives = 205/226 (90.71%), Query Frame = 0


Query: 1   MATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWA 60
           M TE KEMASKA  +VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI  
Sbjct: 1   METEKKEMASKASPSVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 61  LECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTL 120
           +ECPIVILLYSRYR DREQCSYLKAV RGILGL AGA+INAFGAIVLGAP+GAQY  KTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAVARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMS F++VPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFSVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           PLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVK D
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

[Close]

BLAST of Moc08g10020 vs. ExPASy TrEMBL
Match: A0A6J1KCP2 (phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita maxima OX=3661 GN=LOC111493110 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.4e-106
Identity = 193/226 (85.40%), Postives = 202/226 (89.38%), Query Frame = 0


Query: 1   MATE-KEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWA 60
           M TE KEM SKA   VSIAEAF VHLICGLGLALA  IAR++YS DLIS+PS TLFLI  
Sbjct: 1   METEKKEMTSKASPPVSIAEAFFVHLICGLGLALAFLIARHVYSADLISDPSRTLFLILI 60

Query: 61  LECPIVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTL 120
           +ECPIVILLYSRYR DREQCSYLKA+ RGILGL AGA+INAFGAIVLGAP+GAQY  KTL
Sbjct: 61  VECPIVILLYSRYRTDREQCSYLKAMARGILGLLAGAIINAFGAIVLGAPIGAQYLLKTL 120

Query: 121 NWSLVMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180
           NWSLVMS F +VPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM
Sbjct: 121 NWSLVMSSFGVVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPM 180

Query: 181 PLDWERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           PLDWERPWQEWPICVSYGAVIGYS +MVASLAFS  RSGLQHVK D
Sbjct: 181 PLDWERPWQEWPICVSYGAVIGYSIAMVASLAFSHARSGLQHVKRD 226

[Close]

BLAST of Moc08g10020 vs. ExPASy TrEMBL
Match: A0A5A7T0F5 (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00080 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 179/222 (80.63%), Postives = 195/222 (87.84%), Query Frame = 0


Query: 4   EKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECP 63
           +KEMASK+ T +SI EAF +HLI  LGLALA WIA YI+STDLIS+PS TLF I  ++ P
Sbjct: 5   KKEMASKSSTPISIPEAFFLHLISTLGLALAFWIAHYIFSTDLISDPSRTLFFILVVQSP 64

Query: 64  IVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSL 123
           IVILLYSRYR DR QCSYLKAV RG+LGLP GA+INAFGAIVLGAP+GAQYF KTLNWSL
Sbjct: 65  IVILLYSRYRTDRHQCSYLKAVARGLLGLPTGAIINAFGAIVLGAPIGAQYFLKTLNWSL 124

Query: 124 VMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW 183
           VMSLFNIVPSACVFGSSW+DWQRLFAYTKP GTIDHMICIPAHGAIIGAWFGAWPMPLDW
Sbjct: 125 VMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184

Query: 184 ERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           ERPWQEWPICV+YGA++GYS +M ASL  S  R GLQHVK D
Sbjct: 185 ERPWQEWPICVTYGAILGYSIAMAASLVLSHQR-GLQHVKRD 225

[Close]

BLAST of Moc08g10020 vs. ExPASy TrEMBL
Match: A0A0A0L9Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710220 PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 8.2e-99
Identity = 177/222 (79.73%), Postives = 193/222 (86.94%), Query Frame = 0


Query: 4   EKEMASKAPTAVSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECP 63
           +KEMAS + T +SI EAF +HLI  L LALA WIA YI+ST LIS+PS TLFLI  ++ P
Sbjct: 5   KKEMASTSSTPISIPEAFFLHLISALALALAFWIAHYIFSTHLISDPSLTLFLILVVQSP 64

Query: 64  IVILLYSRYRLDREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSL 123
           IVILLYSRYR DR QCSY KAV RG+LGLPAGA+INAFGAIVLGAP+GAQYF KTLNWSL
Sbjct: 65  IVILLYSRYRTDRHQCSYFKAVARGLLGLPAGAIINAFGAIVLGAPIGAQYFLKTLNWSL 124

Query: 124 VMSLFNIVPSACVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDW 183
           VMSLFNIVPSACVFGSSW+DWQRLFAYTKP GTIDHMICIPAHGAIIGAWFGAWPMPLDW
Sbjct: 125 VMSLFNIVPSACVFGSSWIDWQRLFAYTKPIGTIDHMICIPAHGAIIGAWFGAWPMPLDW 184

Query: 184 ERPWQEWPICVSYGAVIGYSTSMVASLAFSRVRSGLQHVKGD 226
           ERPWQEWPICV+YGA++GYS +M ASL  S  R GLQHVK D
Sbjct: 185 ERPWQEWPICVTYGAILGYSIAMAASLVLSHQR-GLQHVKRD 225

[Close]

BLAST of Moc08g10020 vs. TAIR 10
Match: AT1G16040.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic process; LOCATED IN: integral to membrane, endoplasmic reticulum membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GPI biosynthesis protein Pig-F (InterPro:IPR009580); Has 280 Blast hits to 280 proteins in 133 species: Archae - 0; Bacteria - 0; Metazoa - 113; Fungi - 111; Plants - 44; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). )

HSP 1 Score: 246.5 bits (628), Expect = 2.0e-65
Identity = 111/196 (56.63%), Postives = 146/196 (74.49%), Query Frame = 0


Query: 15  VSIAEAFLVHLICGLGLALALWIARYIYSTDLISNPSHTLFLIWALECPIVILLYSRYRL 74
           +S   AF V++I GL L     + R  YS DLIS+P+ TL L+W +E PIV+++YS +R 
Sbjct: 18  ISTWGAFAVYVITGLFLIFGFHVVRNKYSVDLISDPTLTLRLLWIIEFPIVVIIYSLFRR 77

Query: 75  DREQCSYLKAVTRGILGLPAGAVINAFGAIVLGAPVGAQYFSKTLNWSLVMSLFNIVPSA 134
           + E+CSY +AV R ++GL AGA+INA GA+ LGAP+G Q  SKT++WS +MS+F +VP+ 
Sbjct: 78  NPEKCSYFRAVGRSLVGLIAGALINALGAVSLGAPIGMQSLSKTIHWSFLMSVFTVVPAT 137

Query: 135 CVFGSSWMDWQRLFAYTKPNGTIDHMICIPAHGAIIGAWFGAWPMPLDWERPWQEWPICV 194
            V G+SW+DW R+FA  KP G I+HM+ +PA+GAIIG WFGAWPMPLDWERPWQEWPICV
Sbjct: 138 AVLGASWIDWHRIFASLKPIGIIEHMLLVPAYGAIIGGWFGAWPMPLDWERPWQEWPICV 197

Query: 195 SYGAVIGYSTSMVASL 211
            YGA+ GY    + SL
Sbjct: 198 CYGAIGGYIGGQMVSL 213

The following BLAST results are available for this feature:

BLAST of Moc08g10020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153416.1	2.3e-127	100.00	phosphatidylinositol-glycan biosynthesis class F protein [Momordica charantia]	[more]
XP_022949007.1	2.0e-107	86.28	phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita moschata]	[more]
XP_038904140.1	4.5e-107	85.59	phosphatidylinositol-glycan biosynthesis class F protein [Benincasa hispida]	[more]
XP_022998495.1	2.9e-106	85.40	phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita maxima]	[more]
XP_023524788.1	2.9e-106	85.40	phosphatidylinositol-glycan biosynthesis class F protein [Cucurbita pepo subsp. ...	[more]

BLAST of Moc08g10020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y7P2	1.3e-16	33.92	Uncharacterized protein C1450.15 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]
O09101	2.2e-16	36.79	Phosphatidylinositol-glycan biosynthesis class F protein OS=Mus musculus OX=1009...	[more]
Q07326	1.4e-15	26.11	Phosphatidylinositol-glycan biosynthesis class F protein OS=Homo sapiens OX=9606...	[more]
Q6BHK4	9.6e-12	35.40	Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Debaryomyces hans...	[more]
Q6C741	1.3e-11	24.78	Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Yarrowia lipolyti...	[more]

BLAST of Moc08g10020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DKK8	1.1e-127	100.00	phosphatidylinositol-glycan biosynthesis class F protein OS=Momordica charantia ...	[more]
A0A6J1GAT8	9.7e-108	86.28	phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita moschata O...	[more]
A0A6J1KCP2	1.4e-106	85.40	phosphatidylinositol-glycan biosynthesis class F protein OS=Cucurbita maxima OX=...	[more]
A0A5A7T0F5	1.1e-100	80.63	Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Cucumis melo var....	[more]
A0A0A0L9Y3	8.2e-99	79.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G710220 PE=3 SV=1	[more]

BLAST of Moc08g10020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G16040.1	2.0e-65	56.63	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic p...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009580	GPI biosynthesis protein Pig-F	PFAM	PF06699	PIG-F	coord: 39..209 e-value: 3.7E-41 score: 141.3
None	No IPR available	PANTHER	PTHR43157:SF41	BNAA09G56460D PROTEIN	coord: 10..215
None	No IPR available	PANTHER	PTHR43157	PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED	coord: 10..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g10020.1	Moc08g10020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006506	GPI anchor biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051377	mannose-ethanolamine phosphotransferase activity