Moc08g09620 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g09620
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionUbiquitin-conjugating enzyme E2 34
Locationchr8: 7188435 .. 7188997 (+)
RNA-Seq ExpressionMoc08g09620
SyntenyMoc08g09620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAATCTGTTTCTCCTGATCTCGAGATGGATGATCCTGGTTTTGATGAATTTGATCCTCGCCTTAGCTTTTCCAAGGTGATTCACTCCCCTCTTAGATTACTCTGCCATTTTCATTTCTTAACGAAACGTGTTTGTAATTTTACCAGTTCTTGGAAGAAGCAAAACATCACGCCACAGTACAAGATTTCAAAGCCTCAAAGTCAACAGAAGAAGAAGCAGGAAGGAAATGGTTGGGGCAAGAGAAAAAGAGCAAGAAATCATGGAAAACCACCATCTTCTCGTGGTTGAAAAGTGACAAAAAGAGCAAGCCTCTGCCCAAACCAGAAATAAATCATCCTCACACATCCAGTAAAAGACTTGTTCATGTTTCTGGTCCGATTTACACCGGAGCTATGACCACCGACGGCAGACCCCGCCGCCGCCCGACGTCTGGACCGATCGCTAGTCTCTTCAACCCCAGTATGAGGACGGAGATGGAGATCCCTTATATGTGTCTCCACCAGCTTACCAGTCCCAATTCTAGTCATAATTATGGTCCAATTTATCTGGTTACATAG

mRNA sequence

ATGAACAAATCTGTTTCTCCTGATCTCGAGATGGATGATCCTGGTTTTGATGAATTTGATCCTCGCCTTAGCTTTTCCAAGTTCTTGGAAGAAGCAAAACATCACGCCACAGTACAAGATTTCAAAGCCTCAAAGTCAACAGAAGAAGAAGCAGGAAGGAAATGGTTGGGGCAAGAGAAAAAGAGCAAGAAATCATGGAAAACCACCATCTTCTCGTGGTTGAAAAGTGACAAAAAGAGCAAGCCTCTGCCCAAACCAGAAATAAATCATCCTCACACATCCAGTAAAAGACTTGTTCATGTTTCTGGTCCGATTTACACCGGAGCTATGACCACCGACGGCAGACCCCGCCGCCGCCCGACGTCTGGACCGATCGCTAGTCTCTTCAACCCCAGTATGAGGACGGAGATGGAGATCCCTTATATGTGTCTCCACCAGCTTACCAGTCCCAATTCTAGTCATAATTATGGTCCAATTTATCTGGTTACATAG

Coding sequence (CDS)

ATGAACAAATCTGTTTCTCCTGATCTCGAGATGGATGATCCTGGTTTTGATGAATTTGATCCTCGCCTTAGCTTTTCCAAGTTCTTGGAAGAAGCAAAACATCACGCCACAGTACAAGATTTCAAAGCCTCAAAGTCAACAGAAGAAGAAGCAGGAAGGAAATGGTTGGGGCAAGAGAAAAAGAGCAAGAAATCATGGAAAACCACCATCTTCTCGTGGTTGAAAAGTGACAAAAAGAGCAAGCCTCTGCCCAAACCAGAAATAAATCATCCTCACACATCCAGTAAAAGACTTGTTCATGTTTCTGGTCCGATTTACACCGGAGCTATGACCACCGACGGCAGACCCCGCCGCCGCCCGACGTCTGGACCGATCGCTAGTCTCTTCAACCCCAGTATGAGGACGGAGATGGAGATCCCTTATATGTGTCTCCACCAGCTTACCAGTCCCAATTCTAGTCATAATTATGGTCCAATTTATCTGGTTACATAG

Protein sequence

MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEKKSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRPTSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT
Homology
BLAST of Moc08g09620 vs. NCBI nr
Match: XP_022152626.1 (uncharacterized protein LOC111020302 [Momordica charantia])

HSP 1 Score: 332.4 bits (851), Expect = 2.2e-87
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0

Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK
Sbjct: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120
           KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP
Sbjct: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT
Sbjct: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 163

BLAST of Moc08g09620 vs. NCBI nr
Match: XP_038895377.1 (uncharacterized protein LOC120083617 [Benincasa hispida])

HSP 1 Score: 251.5 bits (641), Expect = 4.9e-63
Identity = 128/163 (78.53%), Postives = 139/163 (85.28%), Query Frame = 0

Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KSVSPD EMDDPGFD+ D  L+FSKFL+EAK HA  QDFKA  STEEE GRKWL QEK
Sbjct: 33  MMKSVSPDYEMDDPGFDDIDLSLNFSKFLKEAKRHAIEQDFKA--STEEETGRKWLAQEK 92

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120
           KSKKSWK T+FSWLKS+KK+KP+PKPE N PHT +KR VHVSGPIYTGA TTD RPRR P
Sbjct: 93  KSKKSWKNTLFSWLKSEKKTKPMPKPEKN-PHTPNKRRVHVSGPIYTGATTTDSRPRRHP 152

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNPSMRT+MEIPYM LHQL +PNS+H YGPIYLVT
Sbjct: 153 MSGPIASLFNPSMRTDMEIPYMYLHQLNTPNSNHIYGPIYLVT 192

BLAST of Moc08g09620 vs. NCBI nr
Match: XP_011651731.1 (uncharacterized protein LOC101209455 [Cucumis sativus] >KGN58479.1 hypothetical protein Csa_002575 [Cucumis sativus])

HSP 1 Score: 244.2 bits (622), Expect = 7.8e-61
Identity = 124/163 (76.07%), Postives = 140/163 (85.89%), Query Frame = 0

Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KS+S D EM++PGFD+FD  L+FSKFLEEAKHHAT Q FKA+ S EEE  RKWL QEK
Sbjct: 1   MKKSISLDYEMENPGFDDFDIGLNFSKFLEEAKHHATQQHFKAA-SIEEETERKWLAQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120
           K+KKSWK T+FSWLKSDKKSK LPKPE N PHT +KR V+VSGPIY+ AMT DGRPRRRP
Sbjct: 61  KNKKSWKNTLFSWLKSDKKSKSLPKPEKN-PHTPNKRRVYVSGPIYSEAMTIDGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNP+MRTE EIPYMCLHQL++PN++ NYGPIYLVT
Sbjct: 121 MSGPIASLFNPTMRTETEIPYMCLHQLSAPNTNQNYGPIYLVT 161

BLAST of Moc08g09620 vs. NCBI nr
Match: KAG6606678.1 (hypothetical protein SDJN03_00020, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036398.1 hypothetical protein SDJN02_00015 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 238.0 bits (606), Expect = 5.6e-59
Identity = 122/153 (79.74%), Postives = 129/153 (84.31%), Query Frame = 0

Query: 11  MDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEKKSKKSWKTTI 70
           MDDP FD+FDPR +FSKFLEEAKHHA  +DFKA  ST+EE GRKWL Q KKSKKSWK T+
Sbjct: 1   MDDPCFDDFDPRTNFSKFLEEAKHHAKEEDFKA--STQEETGRKWLAQHKKSKKSWKNTL 60

Query: 71  FSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRPTSGPIASLFN 130
           FSWL SDK SK LPK E N  HTS+KR VHVSGPIYTGA T DGRPR RPTSGPIASLFN
Sbjct: 61  FSWLNSDKTSKSLPKLERN-SHTSNKRRVHVSGPIYTGATTVDGRPRHRPTSGPIASLFN 120

Query: 131 PSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           PSMRTEMEIPYMCLHQLTSP  +H YGPIYLVT
Sbjct: 121 PSMRTEMEIPYMCLHQLTSPIPNHYYGPIYLVT 150

BLAST of Moc08g09620 vs. NCBI nr
Match: XP_022949073.1 (uncharacterized protein LOC111452535 [Cucurbita moschata])

HSP 1 Score: 234.2 bits (596), Expect = 8.1e-58
Identity = 121/153 (79.08%), Postives = 128/153 (83.66%), Query Frame = 0

Query: 11  MDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEKKSKKSWKTTI 70
           MDDP FD+FDPR +FSKFLEEAKHHA  +DFKA  ST+EE GRKWL Q KKSKKSWK T+
Sbjct: 1   MDDPCFDDFDPRTNFSKFLEEAKHHAKEEDFKA--STQEETGRKWLAQHKKSKKSWKNTL 60

Query: 71  FSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRPTSGPIASLFN 130
           FSWL SDK SK LPK E N  HTS+KR VHVSG IYTGA T DGRPR RPTSGPIASLFN
Sbjct: 61  FSWLNSDKTSKSLPKLERN-SHTSNKRRVHVSGLIYTGATTVDGRPRHRPTSGPIASLFN 120

Query: 131 PSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           PSMRTEMEIPYMCLHQLTSP  +H YGPIYLVT
Sbjct: 121 PSMRTEMEIPYMCLHQLTSPIPNHYYGPIYLVT 150

BLAST of Moc08g09620 vs. ExPASy TrEMBL
Match: A0A6J1DIA9 (uncharacterized protein LOC111020302 OS=Momordica charantia OX=3673 GN=LOC111020302 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.1e-87
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0

Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK
Sbjct: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120
           KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP
Sbjct: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT
Sbjct: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 163

BLAST of Moc08g09620 vs. ExPASy TrEMBL
Match: A0A0A0LC20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646660 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.8e-61
Identity = 124/163 (76.07%), Postives = 140/163 (85.89%), Query Frame = 0

Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KS+S D EM++PGFD+FD  L+FSKFLEEAKHHAT Q FKA+ S EEE  RKWL QEK
Sbjct: 1   MKKSISLDYEMENPGFDDFDIGLNFSKFLEEAKHHATQQHFKAA-SIEEETERKWLAQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120
           K+KKSWK T+FSWLKSDKKSK LPKPE N PHT +KR V+VSGPIY+ AMT DGRPRRRP
Sbjct: 61  KNKKSWKNTLFSWLKSDKKSKSLPKPEKN-PHTPNKRRVYVSGPIYSEAMTIDGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNP+MRTE EIPYMCLHQL++PN++ NYGPIYLVT
Sbjct: 121 MSGPIASLFNPTMRTETEIPYMCLHQLSAPNTNQNYGPIYLVT 161

BLAST of Moc08g09620 vs. ExPASy TrEMBL
Match: A0A6J1GB15 (uncharacterized protein LOC111452535 OS=Cucurbita moschata OX=3662 GN=LOC111452535 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 3.9e-58
Identity = 121/153 (79.08%), Postives = 128/153 (83.66%), Query Frame = 0

Query: 11  MDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEKKSKKSWKTTI 70
           MDDP FD+FDPR +FSKFLEEAKHHA  +DFKA  ST+EE GRKWL Q KKSKKSWK T+
Sbjct: 1   MDDPCFDDFDPRTNFSKFLEEAKHHAKEEDFKA--STQEETGRKWLAQHKKSKKSWKNTL 60

Query: 71  FSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRPTSGPIASLFN 130
           FSWL SDK SK LPK E N  HTS+KR VHVSG IYTGA T DGRPR RPTSGPIASLFN
Sbjct: 61  FSWLNSDKTSKSLPKLERN-SHTSNKRRVHVSGLIYTGATTVDGRPRHRPTSGPIASLFN 120

Query: 131 PSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
           PSMRTEMEIPYMCLHQLTSP  +H YGPIYLVT
Sbjct: 121 PSMRTEMEIPYMCLHQLTSPIPNHYYGPIYLVT 150

BLAST of Moc08g09620 vs. ExPASy TrEMBL
Match: A0A5D3D6L0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001220 PE=4 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 4.3e-57
Identity = 121/163 (74.23%), Postives = 136/163 (83.44%), Query Frame = 0

Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KS S D EM++ GFD+FD  L+FS FLEEAKHHAT Q  KA+ S EEE GRK L QEK
Sbjct: 1   MKKSDSLDYEMENTGFDDFDIGLNFSMFLEEAKHHATRQHLKAA-SIEEETGRKRLAQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120
           K+KKSWK T+FSWLKSDKKSK LPKPE N PHT +KR V+VSGP+Y+ A TT+GRPRRRP
Sbjct: 61  KNKKSWKNTLFSWLKSDKKSKSLPKPEKN-PHTPNKRRVYVSGPMYSEATTTNGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNPSMRTE EIPYMCL QL++PNS+HNYGPIYLVT
Sbjct: 121 MSGPIASLFNPSMRTETEIPYMCLQQLSAPNSNHNYGPIYLVT 161

BLAST of Moc08g09620 vs. ExPASy TrEMBL
Match: A0A1S3CDK1 (uncharacterized protein LOC103499209 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499209 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 5.6e-57
Identity = 121/163 (74.23%), Postives = 136/163 (83.44%), Query Frame = 0

Query: 1   MNKSVSPDLEMDDPGFDEFDPRLSFSKFLEEAKHHATVQDFKASKSTEEEAGRKWLGQEK 60
           M KS S D EM++ GFD+FD  L+FS FLEEAKHHAT Q  KA+ S EEE GRK L QEK
Sbjct: 1   MKKSDSLDYEMENTGFDDFDIGLNFSMFLEEAKHHATRQHLKAA-SIEEERGRKRLAQEK 60

Query: 61  KSKKSWKTTIFSWLKSDKKSKPLPKPEINHPHTSSKRLVHVSGPIYTGAMTTDGRPRRRP 120
           K+KKSWK T+FSWLKSDKKSK LPKPE N PHT +KR V+VSGP+Y+ A TT+GRPRRRP
Sbjct: 61  KNKKSWKNTLFSWLKSDKKSKSLPKPEKN-PHTPNKRRVYVSGPMYSEATTTNGRPRRRP 120

Query: 121 TSGPIASLFNPSMRTEMEIPYMCLHQLTSPNSSHNYGPIYLVT 164
            SGPIASLFNPSMRTE EIPYMCL QL++PNS+HNYGPIYLVT
Sbjct: 121 MSGPIASLFNPSMRTETEIPYMCLQQLSAPNSNHNYGPIYLVT 161

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152626.12.2e-87100.00uncharacterized protein LOC111020302 [Momordica charantia][more]
XP_038895377.14.9e-6378.53uncharacterized protein LOC120083617 [Benincasa hispida][more]
XP_011651731.17.8e-6176.07uncharacterized protein LOC101209455 [Cucumis sativus] >KGN58479.1 hypothetical ... [more]
KAG6606678.15.6e-5979.74hypothetical protein SDJN03_00020, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022949073.18.1e-5879.08uncharacterized protein LOC111452535 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DIA91.1e-87100.00uncharacterized protein LOC111020302 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A0A0LC203.8e-6176.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646660 PE=4 SV=1[more]
A0A6J1GB153.9e-5879.08uncharacterized protein LOC111452535 OS=Cucurbita moschata OX=3662 GN=LOC1114525... [more]
A0A5D3D6L04.3e-5774.23Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CDK15.6e-5774.23uncharacterized protein LOC103499209 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..129
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..96
NoneNo IPR availablePANTHERPTHR35488OS05G0358900 PROTEIN-RELATEDcoord: 1..163
NoneNo IPR availablePANTHERPTHR35488:SF4SUBFAMILY NOT NAMEDcoord: 1..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g09620.1Moc08g09620.1mRNA