Moc08g02170 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g02170
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionMethyltransferase
Locationchr8: 1474107 .. 1477508 (+)
RNA-Seq ExpressionMoc08g02170
SyntenyMoc08g02170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAAAGCCTTTGCACCGAGGAGCTTCTGGGGCAAGGATTTGTGAGGGCGGCCATGACTTGGATAGAGCTGAAAACGGAGATTTGGATGACAGAGATTTTTCAAATCATAGTGACATTTTGTGTAGGTTTCCATTTGGATTATCTTCACCCAAAACAACTCCTTCTAAATATGGTTGTCACGAGAATAGCTTCTTGTCTGATTCATTCATAGCGGGAACTCTAAGAAGTCGGCACAAGTTGGCCAAACAAATTTGGAACTTTAGCATACCGATGATTGTACTCGTAATTATCTTTGGATTATTTTGGTGGTCTGTATCCATTTCAATCAAATCCAGACTTCACACTGTTCGTAGTTATGATAAAATACAAGAACAGGTTGTTTTGTACCTTTCTGAATTGGGGAGCTTGCTCTTGGTCCTTCAAGGTTGAAAGAATTGGAATTCTGTTCTCAAGATATTGAAAATCACGTTCCCTGTTTTCGTGTTTCGGATAACCCTGGCTCGGGGTATTCTGATCGTTATTGTGCGCGGGAACCAAAGCAAAATTGCATTGTGCTACCTCCTGTGAAGTACAGGATTCCTCTTAGATGGCCCAGCGGAAGAGATGTAATCTGGTTTGCAAACGTAAGGATTACAGCTGAGGAAGTCCTCTCTTCAGGAAGCTTGACCAAGAGGTTTGTGTACATTAACAATTCTCGTCTATTTAGTCGGTATGACCGTCCTCCGAATATTAACAATAGTTTTGTTTTTACAGAATGATGATGCTGGAGGAAGATCAGATTTCCTTCCGTTCCGACTCTTCCATGTTTGAAGGCATAGAAGATTACTCCCACCAAATTGCAGGGATGATTGGGCTAAGAAATGTATCTAATTTCATACAAGCAGGAGTAAGTAATTCTATTTTTAGATCCAGTAAATTTACTATATTCTGTTTGCTGTCAGCCTGTTGTGTTTTCCCAATTAACTCAAATCATAAAGACTTATTTTGTTCAACCTTGTTCAAGTTTGAAAGAGTTGATGCGTGGAACTTTGCATTTTAACTATGGATGATCATGCTGTTCCTAACATTTTTGCAGATAAGAACTATTCTCGATATTGGATGTGGTTATGGTAGCTTTGGAGCTCATCTCTTTTCGAAACAGCTTTTAACCATGTGCATGGCAAATTATGAAACATCAGGCAGTCAAGTTCAACTAACACTCGAACGAGGTCTTCCAGCAATGTTAGGTTCTTTTGCTTCAAAACAGTTGCCATATCCGTCTCTCTCCTTTGATATGGTTCATTGTGCGCAATGCGGTATTGATTGGGACCTACAAGGTATCTCTCAAAATTTAATACTTCTCATTATATTATTGTAAGTTACACTTCTACATAAATCTTCAAAATCCAATATAATTTATTTTACATATCCATTTTTTCCAACTTACCACATATAAATCTTCCCTTTCGGATTTATTCTTTGAGGTGAAGAATCTTTTTCGAAAAATAGCTCAATAATGGTGGTGGCACAATTTTTCAACTTGTTAACATCACATCTTTTAGTACTTTCAATCAGTCTGCAAATTTGGTTGTATTCCTACTATGGTTTCTGGTCTCTTCCTCCCATCAAGCAAACTCATTTAGAAAGGCAAGCGAGTTCATTGTGTAAACTTTGTGTTATAGGCATGAAGCACGAAATACTTTAGTTTTAGAATCATGTTCTTGACTGGAACATGGATCTCAATAGCTTCTCATTGAACATATCAATTGATCGCGTTGTACAGATGGTATCTATTTAATCGAAGTCGATAGAGTTTTAAGGCCGGGGGGATATTTTGTTTGGACATCACCACTTACCAATGCTGAGAGCTTCCTCCACAACAAAACCAATCAGAAAAGGTGGAACACTATTCGTGATTTTACCGAGGGTCTTTGCTGGGAAATGTTGTCACAACTAGAGAAAACCGTGGTATGGAAAAAAACTAGTGAATCAAGCTGTTATCGTTTGAGGTACACACCATTTATAGAGATCAATTGCTGATCATAAAATTTGTTAATAGCCAAGTTATCCTGATCTTTTGCTATATTCTGCTTGCAATTTTAGGAAATCTGGTTCAGGTCCATCCCTATGTGGTAAAGGGCATTATGTTGAATCTCCATATTATCGACCTCTCGAAGACTGCATCGGTGGAACAAAAAGCTCTAGGTGGATTCCTGTTGAAGAGAGGACATGGCCTTCTAGAGCTAACTTAAACAAGAGTGAACTTGCAGTATATGGTAATAATTTTCTTCTGATATTGATGATTATGTTACTAAGGATGAAGATTTTAACTTAAATGTTTTTATTAGGTGTACAATGGGAAGAATTTGCTGAAGATTCCATCAAATGGAAAATGGCTGTCAATGATTATTGGCCTCTTATGTCGCCACTGATATTCTCAGATCACCCGAAGCGACCCGGGGATGATGACCCTCTGCCCCCATATAACATGCTGCGTAACGTGCTAGACATGAATGCACGGTTTGGAGGTTTTAATTCTGCCCTGTTGGAATCTGGCAAGGATGTATGGGTCATGAACACAGTTCCAACTACTGGATCTAATCACCTTCCGTTGATTGTCGATAGAGGCTTTATCGGTGTATTACATGATTGGTAATGCTACCTTGTCCGATGCCTTTGGATGCGAAGAACGCATTATGATTCGAGCATAGTTTACCTGTTCCTTCCTGTTTATTAATTTATTTTTCCCCTTTCAGGTGTGAAGCATTCCCTTCATATCCTAGAACATATGATATGGTGCATGCAGAGGGTATATTGTCCCTTGAAGCCCTTCGACATCGGTGCACCATGCTAGATATGTTAACTGAGATCGACCGGTTACTTCGTCCCGAGGTAACCAAATAAAAATTTAATGATTAAAACGCTATACTTATAATATAGTTGGGTCATTGTTTGGGTGGATACATTAAAGTGACAAAATAGGATTCCCAAATCTTATTCTTCATCATCATATCATATCTGCTAGTAATTTTGTGTGTAAACTTGAAGTTTTTAATTAGCAACTAATCTTAAATGAACAACAGCCATTCAGGTAATCAACATTTTGATCTGCTGAGATTCTCCAACCTACCTTCCCTTCTGTTTAATCTCTCCCTCTGTCAGATCTGAAATCACTCTAATTTCTTCATTACATCAAATCATCATGTTAAATCAGATGCTGGTTTTTATGTAAGCTAATGTCTTTATGGTTGACTTCAGGGTTGGGTGATAATACATGACACGACAAATCTCATTGAATCGGCTAGAACGCTAACGACACAGCTGAAGTGGGATGCCCGAGTTATCGAGATCGAGAGCGACAATGACAAAAGCCTGCTGGTCTGCCAGAAACCATTCTTAAAGAATATAGCAACTTCCTAG

mRNA sequence

ATGTCAAAGCCTTTGCACCGAGGAGCTTCTGGGGCAAGGATTTGTGAGGGCGGCCATGACTTGGATAGAGCTGAAAACGGAGATTTGGATGACAGAGATTTTTCAAATCATAGTGACATTTTGTGTAGGTTTCCATTTGGATTATCTTCACCCAAAACAACTCCTTCTAAATATGGTTGTCACGAGAATAGCTTCTTGTCTGATTCATTCATAGCGGGAACTCTAAGAAGTCGGCACAAGTTGAAAGAATTGGAATTCTGTTCTCAAGATATTGAAAATCACGTTCCCTGTTTTCGTGTTTCGGATAACCCTGGCTCGGGGTATTCTGATCGTTATTGTGCGCGGGAACCAAAGCAAAATTGCATTGTGCTACCTCCTGTGAAGTACAGGATTCCTCTTAGATGGCCCAGCGGAAGAGATGTAATCTGGTTTGCAAACGTAAGGATTACAGCTGAGGAAGTCCTCTCTTCAGGAAGCTTGACCAAGAGAATGATGATGCTGGAGGAAGATCAGATTTCCTTCCGTTCCGACTCTTCCATGTTTGAAGGCATAGAAGATTACTCCCACCAAATTGCAGGGATGATTGGGCTAAGAAATGTATCTAATTTCATACAAGCAGGAATAAGAACTATTCTCGATATTGGATGTGGTTATGGTAGCTTTGGAGCTCATCTCTTTTCGAAACAGCTTTTAACCATGTGCATGGCAAATTATGAAACATCAGGCAGTCAAGTTCAACTAACACTCGAACGAGGTCTTCCAGCAATGTTAGGTTCTTTTGCTTCAAAACAGTTGCCATATCCGTCTCTCTCCTTTGATATGGTTCATTGTGCGCAATGCGGTATTGATTGGGACCTACAAGATGGTATCTATTTAATCGAAGTCGATAGAGTTTTAAGGCCGGGGGGATATTTTGTTTGGACATCACCACTTACCAATGCTGAGAGCTTCCTCCACAACAAAACCAATCAGAAAAGGTGGAACACTATTCGTGATTTTACCGAGGGTCTTTGCTGGGAAATGTTGTCACAACTAGAGAAAACCGTGGTATGGAAAAAAACTAGTGAATCAAGCTGTTATCGTTTGAGGAAATCTGGTTCAGGTCCATCCCTATGTGGTAAAGGGCATTATGTTGAATCTCCATATTATCGACCTCTCGAAGACTGCATCGGTGGAACAAAAAGCTCTAGGTGGATTCCTGTTGAAGAGAGGACATGGCCTTCTAGAGCTAACTTAAACAAGAGTGAACTTGCAGTATATGGTGTACAATGGGAAGAATTTGCTGAAGATTCCATCAAATGGAAAATGGCTGTCAATGATTATTGGCCTCTTATGTCGCCACTGATATTCTCAGATCACCCGAAGCGACCCGGGGATGATGACCCTCTGCCCCCATATAACATGCTGCGTAACGTGCTAGACATGAATGCACGGTTTGGAGGTTTTAATTCTGCCCTGTTGGAATCTGGCAAGGATGTATGGGTCATGAACACAGTTCCAACTACTGGATCTAATCACCTTCCGTTGATTGTCGATAGAGGCTTTATCGGTGTATTACATGATTGGTGTGAAGCATTCCCTTCATATCCTAGAACATATGATATGGTGCATGCAGAGGGTATATTGTCCCTTGAAGCCCTTCGACATCGGTGCACCATGCTAGATATGTTAACTGAGATCGACCGGTTACTTCGTCCCGAGGGTTGGGTGATAATACATGACACGACAAATCTCATTGAATCGGCTAGAACGCTAACGACACAGCTGAAGTGGGATGCCCGAGTTATCGAGATCGAGAGCGACAATGACAAAAGCCTGCTGGTCTGCCAGAAACCATTCTTAAAGAATATAGCAACTTCCTAG

Coding sequence (CDS)

ATGTCAAAGCCTTTGCACCGAGGAGCTTCTGGGGCAAGGATTTGTGAGGGCGGCCATGACTTGGATAGAGCTGAAAACGGAGATTTGGATGACAGAGATTTTTCAAATCATAGTGACATTTTGTGTAGGTTTCCATTTGGATTATCTTCACCCAAAACAACTCCTTCTAAATATGGTTGTCACGAGAATAGCTTCTTGTCTGATTCATTCATAGCGGGAACTCTAAGAAGTCGGCACAAGTTGAAAGAATTGGAATTCTGTTCTCAAGATATTGAAAATCACGTTCCCTGTTTTCGTGTTTCGGATAACCCTGGCTCGGGGTATTCTGATCGTTATTGTGCGCGGGAACCAAAGCAAAATTGCATTGTGCTACCTCCTGTGAAGTACAGGATTCCTCTTAGATGGCCCAGCGGAAGAGATGTAATCTGGTTTGCAAACGTAAGGATTACAGCTGAGGAAGTCCTCTCTTCAGGAAGCTTGACCAAGAGAATGATGATGCTGGAGGAAGATCAGATTTCCTTCCGTTCCGACTCTTCCATGTTTGAAGGCATAGAAGATTACTCCCACCAAATTGCAGGGATGATTGGGCTAAGAAATGTATCTAATTTCATACAAGCAGGAATAAGAACTATTCTCGATATTGGATGTGGTTATGGTAGCTTTGGAGCTCATCTCTTTTCGAAACAGCTTTTAACCATGTGCATGGCAAATTATGAAACATCAGGCAGTCAAGTTCAACTAACACTCGAACGAGGTCTTCCAGCAATGTTAGGTTCTTTTGCTTCAAAACAGTTGCCATATCCGTCTCTCTCCTTTGATATGGTTCATTGTGCGCAATGCGGTATTGATTGGGACCTACAAGATGGTATCTATTTAATCGAAGTCGATAGAGTTTTAAGGCCGGGGGGATATTTTGTTTGGACATCACCACTTACCAATGCTGAGAGCTTCCTCCACAACAAAACCAATCAGAAAAGGTGGAACACTATTCGTGATTTTACCGAGGGTCTTTGCTGGGAAATGTTGTCACAACTAGAGAAAACCGTGGTATGGAAAAAAACTAGTGAATCAAGCTGTTATCGTTTGAGGAAATCTGGTTCAGGTCCATCCCTATGTGGTAAAGGGCATTATGTTGAATCTCCATATTATCGACCTCTCGAAGACTGCATCGGTGGAACAAAAAGCTCTAGGTGGATTCCTGTTGAAGAGAGGACATGGCCTTCTAGAGCTAACTTAAACAAGAGTGAACTTGCAGTATATGGTGTACAATGGGAAGAATTTGCTGAAGATTCCATCAAATGGAAAATGGCTGTCAATGATTATTGGCCTCTTATGTCGCCACTGATATTCTCAGATCACCCGAAGCGACCCGGGGATGATGACCCTCTGCCCCCATATAACATGCTGCGTAACGTGCTAGACATGAATGCACGGTTTGGAGGTTTTAATTCTGCCCTGTTGGAATCTGGCAAGGATGTATGGGTCATGAACACAGTTCCAACTACTGGATCTAATCACCTTCCGTTGATTGTCGATAGAGGCTTTATCGGTGTATTACATGATTGGTGTGAAGCATTCCCTTCATATCCTAGAACATATGATATGGTGCATGCAGAGGGTATATTGTCCCTTGAAGCCCTTCGACATCGGTGCACCATGCTAGATATGTTAACTGAGATCGACCGGTTACTTCGTCCCGAGGGTTGGGTGATAATACATGACACGACAAATCTCATTGAATCGGCTAGAACGCTAACGACACAGCTGAAGTGGGATGCCCGAGTTATCGAGATCGAGAGCGACAATGACAAAAGCCTGCTGGTCTGCCAGAAACCATTCTTAAAGAATATAGCAACTTCCTAG

Protein sequence

MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGCHENSFLSDSFIAGTLRSRHKLKELEFCSQDIENHVPCFRVSDNPGSGYSDRYCAREPKQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSDSSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMANYETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDRVLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSESSCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSELAVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNARFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVHAEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIEIESDNDKSLLVCQKPFLKNIATS
Homology
BLAST of Moc08g02170 vs. NCBI nr
Match: XP_022132200.1 (probable pectin methyltransferase QUA2 [Momordica charantia])

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 617/683 (90.34%), Postives = 617/683 (90.34%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC 60
           MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC
Sbjct: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC 60

Query: 61  HENSFLSDSFIAGTLRSRHK---------------------------------------- 120
           HENSFLSDSFIAGTLRSRHK                                        
Sbjct: 61  HENSFLSDSFIAGTLRSRHKLAKQIXNFSIPMIVLVIIFGLFWWSVSISIKSRLHTVRSY 120

Query: 121 -----------------------LKELEFCSQDIENHVPCFRVSDNPGSGYSDRYCAREP 180
                                  LKELEFC QDIENHVPCF VSDNPGSGYSDRYCAREP
Sbjct: 121 DKIQEQVVLYLSXIGELALGPSRLKELEFCXQDIENHVPCFXVSDNPGSGYSDRYCAREP 180

Query: 181 KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD 240
           KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD
Sbjct: 181 KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD 240

Query: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN 300
           SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN
Sbjct: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN 300

Query: 301 YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR 360
           YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR
Sbjct: 301 YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR 360

Query: 361 VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES 420
           VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES
Sbjct: 361 VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES 420

Query: 421 SCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL 480
           SCYR RKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL
Sbjct: 421 SCYRXRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL 480

Query: 481 AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA 540
           AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA
Sbjct: 481 AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA 540

Query: 541 RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH 600
           RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH
Sbjct: 541 RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH 600

Query: 601 AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE 621
           AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE
Sbjct: 601 AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE 660

BLAST of Moc08g02170 vs. NCBI nr
Match: XP_023546073.1 (probable pectin methyltransferase QUA2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1057.0 bits (2732), Expect = 6.4e-305
Identity = 510/683 (74.67%), Postives = 549/683 (80.38%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC 60
           M KPLHRG SG RICEG  DL+  E GD D +  S  SD + R                 
Sbjct: 1   MLKPLHRGVSGTRICEGSRDLNGTEKGDFDAKCSSYESDEMFR----------------- 60

Query: 61  HENSFLSDSFIAGTLRSRHK---------------------------------------- 120
               FLSDSF+ GTLR++HK                                        
Sbjct: 61  ----FLSDSFVLGTLRNQHKLAKQMLSFSIPLIVLVIFFGLFWWSVSISTSSRLHAARSY 120

Query: 121 -----------------------LKELEFCSQDIENHVPCFRVSDNPGSGYSDRYCAREP 180
                                  L+ELEFCSQD ENHVPCF   +NP SGY+DR C  EP
Sbjct: 121 DKIQEQVVLYLSEIGELALGPSRLRELEFCSQDFENHVPCFSARENPDSGYADRSCELEP 180

Query: 181 KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD 240
           KQNCIV PPVKYR+PLRWP GRDVIWFANV++TA+EVLSSGS+TKRMMM+EE+QISFRSD
Sbjct: 181 KQNCIVPPPVKYRVPLRWPIGRDVIWFANVKMTADEVLSSGSMTKRMMMMEEEQISFRSD 240

Query: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN 300
           SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMC+AN
Sbjct: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCIAN 300

Query: 301 YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR 360
           YETSGSQVQLTLERGLPAML SFASKQLPYPS SFDMVHCAQCGIDWDL+DGIYL EVDR
Sbjct: 301 YETSGSQVQLTLERGLPAMLSSFASKQLPYPSFSFDMVHCAQCGIDWDLKDGIYLTEVDR 360

Query: 361 VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES 420
           VLRPGGYFVW SP+ +A+SFLHNKTN K+WN +RDFTE LCWEMLSQL+KTVVWKKTS+S
Sbjct: 361 VLRPGGYFVWMSPIADAQSFLHNKTNLKKWNAVRDFTESLCWEMLSQLDKTVVWKKTSKS 420

Query: 421 SCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL 480
           SCY  RK  SGP LC KGHYVESPYYRPLE+CIGGTKSSRW PVE+RTWPSRANLNKS+L
Sbjct: 421 SCYHSRKPSSGPPLCRKGHYVESPYYRPLENCIGGTKSSRWFPVEQRTWPSRANLNKSKL 480

Query: 481 AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA 540
           AVYGVQWE+FAEDS+KWKMAVNDYWPLMSPLIFSDHPKRPGDDDP PPYNMLRNVLDMNA
Sbjct: 481 AVYGVQWEDFAEDSMKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPSPPYNMLRNVLDMNA 540

Query: 541 RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH 600
           +FGGFNSALLESGKDVWVMN VPTTGSNHLPLIVDRGF+GVLHDWCEAFP+YPRTYDMVH
Sbjct: 541 QFGGFNSALLESGKDVWVMNAVPTTGSNHLPLIVDRGFLGVLHDWCEAFPTYPRTYDMVH 600

Query: 601 AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE 621
           A+GILSLEALRHRC+MLD+L+EIDRLLRPEGWVIIHDTTNLIESAR LTTQLKWDARVIE
Sbjct: 601 ADGILSLEALRHRCSMLDLLSEIDRLLRPEGWVIIHDTTNLIESARMLTTQLKWDARVIE 660

BLAST of Moc08g02170 vs. NCBI nr
Match: XP_022961962.1 (probable pectin methyltransferase QUA2 [Cucurbita moschata] >XP_022961963.1 probable pectin methyltransferase QUA2 [Cucurbita moschata])

HSP 1 Score: 1053.1 bits (2722), Expect = 9.2e-304
Identity = 509/683 (74.52%), Postives = 549/683 (80.38%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC 60
           M KPLHRG SG RICEG  DL+  E GD D +  S  SD + R                 
Sbjct: 1   MLKPLHRGVSGTRICEGSRDLNGTEKGDFDAKCSSYESDEMFR----------------- 60

Query: 61  HENSFLSDSFIAGTLRSRHK---------------------------------------- 120
               FLSDSF+ GTLR++HK                                        
Sbjct: 61  ----FLSDSFVLGTLRNQHKLAKQMLSFSIPLIVIVIFFGLFWWSVSISTSSRLHAARSY 120

Query: 121 -----------------------LKELEFCSQDIENHVPCFRVSDNPGSGYSDRYCAREP 180
                                  L+ELEFCSQD ENHVPCF   +NP SGY+DR C  EP
Sbjct: 121 DKIQEQVVLYLSEIGELALGPSRLRELEFCSQDFENHVPCFSARENPDSGYADRSCELEP 180

Query: 181 KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD 240
           KQNCIV PPVKYR+PLRWP GRDVIWFANV++TA+EVLSSGS+TKRMMM+EE+QISFRSD
Sbjct: 181 KQNCIVPPPVKYRVPLRWPIGRDVIWFANVKMTADEVLSSGSMTKRMMMMEEEQISFRSD 240

Query: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN 300
           SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMC+AN
Sbjct: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCVAN 300

Query: 301 YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR 360
           YETSGSQVQLTLERGLPAML +FASKQLPYPS SFDMVHCAQCGIDWDL+DGIYL EVDR
Sbjct: 301 YETSGSQVQLTLERGLPAMLSTFASKQLPYPSFSFDMVHCAQCGIDWDLKDGIYLTEVDR 360

Query: 361 VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES 420
           VLRPGGYFVWTSP+ +A+SFLHNKTN K+WN +RDFTE LCWEMLSQL+KTVVWKKTS+S
Sbjct: 361 VLRPGGYFVWTSPIADAQSFLHNKTNLKKWNAVRDFTESLCWEMLSQLDKTVVWKKTSKS 420

Query: 421 SCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL 480
           SCY  RK  SGP LC KGHYVESPYYRPLE+CIGGTKSSRW PVE+RTWPSRANLNKS+L
Sbjct: 421 SCYHSRKPRSGPPLCSKGHYVESPYYRPLENCIGGTKSSRWFPVEQRTWPSRANLNKSKL 480

Query: 481 AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA 540
           AVYGVQ E+FAEDS+KWKMAVNDYWPLMSPLIFSDHPKRPGDDDP PPYNMLRNVLDMNA
Sbjct: 481 AVYGVQREDFAEDSMKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPTPPYNMLRNVLDMNA 540

Query: 541 RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH 600
           +FGGFNSALLESGKDVWVMN VPTTGSNHLPLIVDRGF+GVLHDWCEAFP+YPRTYDMVH
Sbjct: 541 QFGGFNSALLESGKDVWVMNAVPTTGSNHLPLIVDRGFLGVLHDWCEAFPTYPRTYDMVH 600

Query: 601 AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE 621
           A+GILSLEALRHRC+MLD+L+EIDRLLRPEGWVIIHDTTNLIESAR LTTQLKWDARVIE
Sbjct: 601 ADGILSLEALRHRCSMLDLLSEIDRLLRPEGWVIIHDTTNLIESARMLTTQLKWDARVIE 660

BLAST of Moc08g02170 vs. NCBI nr
Match: XP_022997075.1 (probable pectin methyltransferase QUA2 [Cucurbita maxima])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.3e-302
Identity = 505/683 (73.94%), Postives = 547/683 (80.09%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC 60
           M KPLHRG SG RICEG HDL+  E GD D +  S  SD + R                 
Sbjct: 1   MLKPLHRGVSGTRICEGSHDLNGTEKGDFDVKGSSYESDEMFR----------------- 60

Query: 61  HENSFLSDSFIAGTLRSRHK---------------------------------------- 120
               FLSDSF+ GTLR++HK                                        
Sbjct: 61  ----FLSDSFVLGTLRNQHKLAKQMLNFSIPLIVLVIFFGLFWWSVSISTSSRLHAARSY 120

Query: 121 -----------------------LKELEFCSQDIENHVPCFRVSDNPGSGYSDRYCAREP 180
                                  L+ELEFCSQD ENHVPCF   +NP SG +DR C  EP
Sbjct: 121 DKIQEQVVLYLSEIGELALGPSRLRELEFCSQDFENHVPCFSARENPDSGSADRSCEVEP 180

Query: 181 KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD 240
           KQNCIV PPVKYR+PLRWP GRDVIWFANV++TA+EVLSSGS+TKRMMM+EE+QISFRSD
Sbjct: 181 KQNCIVPPPVKYRVPLRWPIGRDVIWFANVKMTADEVLSSGSMTKRMMMMEEEQISFRSD 240

Query: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN 300
           SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMC+AN
Sbjct: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCIAN 300

Query: 301 YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR 360
           YETSGSQVQLTLERGLPAML SFASKQLPYPS SFDMVHCAQCGIDWDL+DGIYL EVDR
Sbjct: 301 YETSGSQVQLTLERGLPAMLSSFASKQLPYPSFSFDMVHCAQCGIDWDLKDGIYLTEVDR 360

Query: 361 VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES 420
           VLRPGGYFVW SP+ +A+SFLHNKTN K+WN +RDFT+ LCWEMLSQL+KTVVWKKTS+S
Sbjct: 361 VLRPGGYFVWMSPIADAQSFLHNKTNLKKWNAVRDFTKSLCWEMLSQLDKTVVWKKTSKS 420

Query: 421 SCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL 480
           SCY  RK  SGP LC KGHYVESPYYRPLE+CIGGTKSSRW PVE+RTWPSRANLNKS+L
Sbjct: 421 SCYHSRKPSSGPPLCNKGHYVESPYYRPLENCIGGTKSSRWFPVEQRTWPSRANLNKSKL 480

Query: 481 AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA 540
           AVYGVQWE+FAEDS+KWKMAVNDYWPLMSPLIFSDHPKRPG+DDP PPYNMLRNVLDMNA
Sbjct: 481 AVYGVQWEDFAEDSMKWKMAVNDYWPLMSPLIFSDHPKRPGEDDPTPPYNMLRNVLDMNA 540

Query: 541 RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH 600
           +FGGFNSALLESGKDVWVMN VPTTGSNHLPLIVDRGF+GVLHDWCEAFP+YPRTYDMVH
Sbjct: 541 QFGGFNSALLESGKDVWVMNAVPTTGSNHLPLIVDRGFLGVLHDWCEAFPTYPRTYDMVH 600

Query: 601 AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE 621
           A+ ILSLEA+RHRC+MLD+L+EIDRLLRPEGWVIIHD TNLIESAR LTTQLKWDARVIE
Sbjct: 601 ADSILSLEAVRHRCSMLDLLSEIDRLLRPEGWVIIHDATNLIESARRLTTQLKWDARVIE 660

BLAST of Moc08g02170 vs. NCBI nr
Match: KAG6598691.1 (putative pectin methyltransferase QUA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.5e-295
Identity = 472/541 (87.25%), Postives = 507/541 (93.72%), Query Frame = 0

Query: 80  KLKELEFCSQDIENHVPCFRVSDNPGSGYSDRYCAREPKQNCIVLPPVKYRIPLRWPSGR 139
           +L+ELEFCSQD ENHVPCF   +NP SGY+DR C  EPKQNCIV PPVKYR+PLRWP GR
Sbjct: 119 RLRELEFCSQDFENHVPCFSARENPDSGYADRSCELEPKQNCIVPPPVKYRVPLRWPIGR 178

Query: 140 DVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSDSSMFEGIEDYSHQIAGMIGLRN 199
           DVIWFANV++TA+EVLSSGS+TKRMMM+EE+QISFRSDSSMFEGIEDYSHQIAGMIGLRN
Sbjct: 179 DVIWFANVKMTADEVLSSGSMTKRMMMMEEEQISFRSDSSMFEGIEDYSHQIAGMIGLRN 238

Query: 200 VSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMANYETSGSQVQLTLERGLPAMLGS 259
           VSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMC+ANYETSGSQVQLTLERGLPAML S
Sbjct: 239 VSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCVANYETSGSQVQLTLERGLPAMLSS 298

Query: 260 FASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDRVLRPGGYFVWTSPLTNAESFLH 319
           FASKQLPYPS SFDMVHCAQCGIDWDL+DGIYL EVDRVLRPGGYFVW SP+ +A+SFLH
Sbjct: 299 FASKQLPYPSFSFDMVHCAQCGIDWDLKDGIYLTEVDRVLRPGGYFVWMSPIADAQSFLH 358

Query: 320 NKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSESSCYRLRKSGSGPSLCGKGHYVE 379
           NKTN K+WN +RDFTE LCWEMLSQL+KTVVWKKTS+SSCY  RK  SGP LC KGHYVE
Sbjct: 359 NKTNLKKWNAVRDFTESLCWEMLSQLDKTVVWKKTSKSSCYHSRKPRSGPPLCSKGHYVE 418

Query: 380 SPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSELAVYGVQWEEFAEDSIKWKMAVN 439
           SPYYRPLE+CIGGTKSSRW PVE+RTWPSRANLNKS+LAVYGVQWE+FAEDS+KWKMAVN
Sbjct: 419 SPYYRPLENCIGGTKSSRWFPVEQRTWPSRANLNKSKLAVYGVQWEDFAEDSMKWKMAVN 478

Query: 440 DYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNARFGGFNSALLESGKDVWVMNTV 499
           DYWPLMSPLIFSDHPKRPGDDDP PPYNMLRNVLDMNA+FGGFNSALLESGK+VWVMN V
Sbjct: 479 DYWPLMSPLIFSDHPKRPGDDDPTPPYNMLRNVLDMNAQFGGFNSALLESGKNVWVMNAV 538

Query: 500 PTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVHAEGILSLEALRHRCTMLDMLTE 559
           PTTGSNHLPLIVDRGF+GVLHDWCEAFP+YPRTYDMVHA+GILSLEALRHRC+MLD+L+E
Sbjct: 539 PTTGSNHLPLIVDRGFLGVLHDWCEAFPTYPRTYDMVHADGILSLEALRHRCSMLDLLSE 598

Query: 560 IDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIEIESDNDKSLLVCQKPFLKNIAT 619
           IDRLLRPEGWVIIHD TNLIESAR LTTQLKWDARVIE +S+ DK LLVCQKPFLK IA 
Sbjct: 599 IDRLLRPEGWVIIHDATNLIESARMLTTQLKWDARVIESDSNMDKRLLVCQKPFLKKIAN 658

Query: 620 S 621
           S
Sbjct: 659 S 659

BLAST of Moc08g02170 vs. ExPASy Swiss-Prot
Match: Q9C9Q8 (Probable pectin methyltransferase QUA2 OS=Arabidopsis thaliana OX=3702 GN=QUA2 PE=1 SV=2)

HSP 1 Score: 825.9 bits (2132), Expect = 3.1e-238
Identity = 400/688 (58.14%), Postives = 492/688 (71.51%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFS-NHSDILCRFPFGLSSPKTTPSKY- 60
           MS PL RG SG R+ +   DL  ++  D  +R  S  ++++  RFPFG      + SK+ 
Sbjct: 1   MSMPLQRGISGVRVSDSSDDLRDSQMKDKTERARSTENNNLTLRFPFGFLFSNQSSSKHG 60

Query: 61  GCHENSFLSDSFIAGTLRSRHKL------------------------------------- 120
           G  EN F +D + A   RSRH+L                                     
Sbjct: 61  GGGENGFSADPYSA---RSRHRLMLLFLKISLVLIVVIALAGSFWWTISISTSSRGHVYH 120

Query: 121 --------------------------KELEFCSQDIENHVPCFRVSDNPGSGYS-----D 180
                                     KELE+C+ + EN VPCF VS+N   GYS     D
Sbjct: 121 NYRRLQEQLVSDLWDIGEISLGPNRWKELEYCNIESENFVPCFNVSENLALGYSNGDEND 180

Query: 181 RYCAREPKQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEED 240
           R+C    KQ C+ LPPVKYR+PLRWP+G+D+IW +NV+ITA+EV+SSGS+TKRMMM+E+D
Sbjct: 181 RFCGPGSKQECLELPPVKYRVPLRWPTGKDIIWHSNVKITAQEVVSSGSITKRMMMMEDD 240

Query: 241 QISFRSDSSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQL 300
           QISFRS S M + +EDYSHQIA MIG++   NFI+AG+RTILDIGCGYGSFGAHL SKQ+
Sbjct: 241 QISFRSASPMSDEVEDYSHQIAEMIGIKK-DNFIEAGVRTILDIGCGYGSFGAHLLSKQI 300

Query: 301 LTMCMANYETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGI 360
           LTMC+ANYE SGSQVQLTLERGLPAM+GSF SKQLPYPSLSFDM+HC +CGIDWD +DG+
Sbjct: 301 LTMCIANYEASGSQVQLTLERGLPAMIGSFISKQLPYPSLSFDMLHCLRCGIDWDQKDGL 360

Query: 361 YLIEVDRVLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVV 420
            L+E+DRVL+PGGYFVWTSPLTN      NK + KRWN + DF E +CW +L+Q ++TVV
Sbjct: 361 LLVEIDRVLKPGGYFVWTSPLTNP----RNKDHLKRWNFVHDFAESICWTLLNQQDETVV 420

Query: 421 WKKTSESSCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERT-WPSR 480
           WKKT  + CY  RK G GPS+C KGH VESPYYRPL+ CIGGT+S RWIP+E RT WPSR
Sbjct: 421 WKKTINTKCYSSRKPGVGPSVCTKGHDVESPYYRPLQMCIGGTRSRRWIPIEGRTRWPSR 480

Query: 481 ANLNKSELAVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNML 540
           +N+NK+EL++YG+  E   ED+  WK+ V +YW L+SPLIFSDHPKRPGD+DP PPYNML
Sbjct: 481 SNMNKTELSLYGLHPEVLGEDAENWKITVREYWSLLSPLIFSDHPKRPGDEDPSPPYNML 540

Query: 541 RNVLDMNARFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSY 600
           RNVLDMNA+FGG NSALLE+ K VWVMN VPT G NHLP+I+DRGF+GVLH+WCE FP+Y
Sbjct: 541 RNVLDMNAQFGGLNSALLEARKSVWVMNVVPTAGPNHLPMILDRGFVGVLHNWCEPFPTY 600

Query: 601 PRTYDMVHAEGILSLEALRHR--CTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTT 616
           PRTYD+VHA+ +LSL+  + R  C ++D+ TEIDRLLRPEGWVII DT  L+E AR   T
Sbjct: 601 PRTYDLVHADNLLSLQTSQPRKTCLLIDIFTEIDRLLRPEGWVIIRDTAQLVEKARETIT 660

BLAST of Moc08g02170 vs. ExPASy Swiss-Prot
Match: Q8GYW9 (Probable methyltransferase PMT4 OS=Arabidopsis thaliana OX=3702 GN=At1g13860 PE=2 SV=2)

HSP 1 Score: 636.3 bits (1640), Expect = 3.5e-181
Identity = 294/540 (54.44%), Postives = 397/540 (73.52%), Query Frame = 0

Query: 79  HKLKELEFCSQDIENHVPCFRVSDNPGSGYSDRYC--AREPKQNCIVLPPVKYRIPLRWP 138
           ++LKE   C ++ +N+VPC+ V++      SDR C  ARE ++ C+V PP  Y+IPLRWP
Sbjct: 76  NRLKEFPLCGKERDNYVPCYNVTE------SDRNCEFARE-EERCLVRPPRDYKIPLRWP 135

Query: 139 SGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSDSSM-FEGIEDYSHQIAGMI 198
            GRD+IW  NV+IT ++ LSSG++TKR+M+LEE+QI+F SD  + F+G++DY+ QIA MI
Sbjct: 136 VGRDIIWTGNVKITKDQFLSSGTMTKRLMLLEENQITFHSDDGLIFDGVKDYAFQIAEMI 195

Query: 199 GLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMANYETSGSQVQLTLERGLPA 258
           GL + + F QAGIRT+LDIGCG+GSFGAHL S  ++ +C+A YETSGSQVQL LERGLPA
Sbjct: 196 GLGSDTEFPQAGIRTVLDIGCGFGSFGAHLVSLNVMPICIAEYETSGSQVQLALERGLPA 255

Query: 259 MLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDRVLRPGGYFVWTSPLTNAE 318
           M+G+F SKQLPYP+LSFDMVHCAQCGI WD++D + L+EVDRVL+PGGYFV TSP + A+
Sbjct: 256 MIGNFFSKQLPYPALSFDMVHCAQCGITWDIKDAMLLLEVDRVLKPGGYFVLTSPTSKAQ 315

Query: 319 SFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSESSCYRLRKSGSGPSLCGKG 378
               +         + + ++ +CW +  Q ++T +W+KT++ +CY  R   S P +C   
Sbjct: 316 GNSPDTKKTSISTRVDELSKKICWSLSGQQDETFLWQKTADPNCYSSRSQASIP-VCKDD 375

Query: 379 HYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSELAVYGVQWEEFAEDSIKWK 438
             V  PYY PL  CI GTKS RWIP++ R+  S  +L  SEL ++G++ EEF ED   W+
Sbjct: 376 DSV--PYYHPLVPCISGTKSKRWIPIQNRSRASGTSL--SELEIHGIKPEEFDEDIQVWR 435

Query: 439 MAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNARFGGFNSALLESGKDVWV 498
            A+ +YW L++PLIFSDHPKRPGD+DP+PP+ M+RN +DMNAR+G  N ALL  GK VWV
Sbjct: 436 SALKNYWSLLTPLIFSDHPKRPGDEDPVPPFYMIRNAMDMNARYGNLNQALLNQGKSVWV 495

Query: 499 MNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVHAEGILSLEALRHRCTMLD 558
           MN VP    N LP+I+DRGF G LHDWCE FP+YPRTYDM+HA  +L+      RC+++D
Sbjct: 496 MNVVPVKARNTLPIILDRGFTGALHDWCEPFPTYPRTYDMLHANELLT-HLSSERCSLMD 555

Query: 559 MLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIEIESDNDKSLLVCQKPFLK 616
           +  E+DR+LRPEGWV++ D   +IE ARTL  +++W+ARVI+I+  +D+ LLVCQKP LK
Sbjct: 556 LFLEMDRILRPEGWVVLSDKLGVIEMARTLAARVRWEARVIDIQDGSDQRLLVCQKPLLK 602

BLAST of Moc08g02170 vs. ExPASy Swiss-Prot
Match: Q3EC77 (Probable methyltransferase PMT5 OS=Arabidopsis thaliana OX=3702 GN=At2g03480 PE=2 SV=2)

HSP 1 Score: 620.5 bits (1599), Expect = 2.0e-176
Identity = 287/542 (52.95%), Postives = 389/542 (71.77%), Query Frame = 0

Query: 81  LKELEFCSQDIENHVPCFRVSDNPGSGYS-----DRYCARE-PKQNCIVLPPVKYRIPLR 140
           LKE  FC ++ E++VPC+ ++ N  +G       DR+C  E  K+ C+V PP  Y+IPLR
Sbjct: 83  LKEFPFCGKERESYVPCYNITGNLLAGLQEGEELDRHCEFEREKERCVVRPPRDYKIPLR 142

Query: 141 WPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRS-DSSMFEGIEDYSHQIAG 200
           WP GRD+IW  NV+IT ++ LSSG++T R+M+LEE+QI+F S D  +F+G++DY+ QIA 
Sbjct: 143 WPLGRDIIWSGNVKITKDQFLSSGTVTTRLMLLEENQITFHSEDGLVFDGVKDYARQIAE 202

Query: 201 MIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMANYETSGSQVQLTLERGL 260
           MIGL + + F QAG+RT+LDIGCG+GSFGAHL S +L+ +C+A YE +GSQVQL LERGL
Sbjct: 203 MIGLGSDTEFAQAGVRTVLDIGCGFGSFGAHLVSLKLMPICIAEYEATGSQVQLALERGL 262

Query: 261 PAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDRVLRPGGYFVWTSPLTN 320
           PAM+G+F SKQLPYP+LSFDMVHCAQCG  WD++D + L+EVDRVL+PGGYFV TSP   
Sbjct: 263 PAMIGNFFSKQLPYPALSFDMVHCAQCGTTWDIKDAMLLLEVDRVLKPGGYFVLTSPTNK 322

Query: 321 AESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSESSCYRLRKSGSGPSLCG 380
           A+  L +         + + ++ +CW + +Q ++T +W+KTS+SSCY  R   S P LC 
Sbjct: 323 AQGNLPDTKKTSISTRVNELSKKICWSLTAQQDETFLWQKTSDSSCYSSRSQASIP-LCK 382

Query: 381 KGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSELAVYGVQWEEFAEDSIK 440
            G  V  PYY PL  CI GT S RWI ++ R+  + A    + L ++G            
Sbjct: 383 DGDSV--PYYHPLVPCISGTTSKRWISIQNRS--AVAGTTSAGLEIHG------------ 442

Query: 441 WKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNARFGGFNSALLESGKDV 500
            K A+ +YW L++PLIFSDHPKRPGD+DPLPP+NM+RNV+DM+ARFG  N+ALL+ GK  
Sbjct: 443 -KSALKNYWSLLTPLIFSDHPKRPGDEDPLPPFNMIRNVMDMHARFGNLNAALLDEGKSA 502

Query: 501 WVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVHAEGILSLEALRHRCTM 560
           WVMN VP    N LP+I+DRGF GVLHDWCE FP+YPRTYDM+HA  +L+      RC++
Sbjct: 503 WVMNVVPVNARNTLPIILDRGFAGVLHDWCEPFPTYPRTYDMLHANELLT-HLSSERCSL 562

Query: 561 LDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIEIESDNDKSLLVCQKPF 616
           +D+  E+DR+LRPEGWV++ D   +IE AR L  +++W+ARVI+++  +D+ LLVCQKPF
Sbjct: 563 MDLFLEMDRILRPEGWVVLSDKVGVIEMARALAARVRWEARVIDLQDGSDQRLLVCQKPF 605

BLAST of Moc08g02170 vs. ExPASy Swiss-Prot
Match: Q8VZV7 (Probable methyltransferase PMT9 OS=Arabidopsis thaliana OX=3702 GN=At5g14430 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.5e-102
Identity = 194/507 (38.26%), Postives = 288/507 (56.80%), Query Frame = 0

Query: 116 EPKQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFR 175
           E + NC+V PPV Y+IPLRWP  RD +W AN+  T    L+     +  M++  D+I+F 
Sbjct: 122 ERRFNCLVPPPVGYKIPLRWPVSRDEVWKANIPHTH---LAQEKSDQNWMVVNGDKINFP 181

Query: 176 SDSSMF-EGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMC 235
              + F  G + Y   +A M+            IR +LD+GCG  SFGA+L S  ++ M 
Sbjct: 182 GGGTHFHNGADKYIVSLAQMLKFPGDKLNNGGSIRNVLDVGCGVASFGAYLLSHDIIAMS 241

Query: 236 MANYETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIE 295
           +A  +   +Q+Q  LERG+P+ LG   +K+LPYPS SF++ HC++C IDW  +DGI L+E
Sbjct: 242 LAPNDVHQNQIQFALERGIPSTLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLE 301

Query: 296 VDRVLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKT 355
           +DR+LRPGGYFV++SP    E++ H+  N+K  N + D  + +CW+++++ +++V+W K 
Sbjct: 302 LDRLLRPGGYFVYSSP----EAYAHDPENRKIGNAMHDLFKRMCWKVVAKRDQSVIWGKP 361

Query: 356 SESSCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIG----GTKSSRWIPVEERTWPSRA 415
             +SCY  R  G  P LC  G   ++ +   ++ CI          RW  +    WP R 
Sbjct: 362 ISNSCYLKRDPGVLPPLCPSGDDPDATWNVSMKACISPYSVRMHKERWSGLV--PWPRRL 421

Query: 416 NLNKSELAVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLR 475
                 L   GV  E+F ED+  W++ V +YW L+ P++                 N +R
Sbjct: 422 TAPPPRLEEIGVTPEQFREDTETWRLRVIEYWKLLKPMV---------------QKNSIR 481

Query: 476 NVLDMNARFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYP 535
           NV+DM++  GGF +AL  + KDVWVMN +P   S  + +I DRG IG  HDWCEAF +YP
Sbjct: 482 NVMDMSSNLGGFAAAL--NDKDVWVMNVMPVQSSPRMKIIYDRGLIGATHDWCEAFDTYP 541

Query: 536 RTYDMVHAEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLK 595
           RT+D++HA    + E     C+  D+L E+DR+LRPEG+VII DTT+ I   +   T LK
Sbjct: 542 RTFDLIHAWNTFT-ETQARGCSFEDLLIEMDRILRPEGFVIIRDTTDNISYIKKYLTLLK 601

Query: 596 WDARVIEIE------SDNDKSLLVCQK 612
           WD    E        S  D+ +L+ +K
Sbjct: 602 WDKWSTETTPKGDPLSTKDEIVLIARK 601

BLAST of Moc08g02170 vs. ExPASy Swiss-Prot
Match: Q8H118 (Probable methyltransferase PMT1 OS=Arabidopsis thaliana OX=3702 GN=At3g23300 PE=2 SV=2)

HSP 1 Score: 365.2 bits (936), Expect = 1.5e-99
Identity = 190/517 (36.75%), Postives = 293/517 (56.67%), Query Frame = 0

Query: 110 DRYC-AREPKQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLE 169
           +R+C   E + NC++ PP  Y+IP++WP  RD +W  N+  T    L+     +  M+++
Sbjct: 114 ERHCPPPERRFNCLIPPPPGYKIPIKWPKSRDEVWKVNIPHTH---LAHEKSDQNWMVVK 173

Query: 170 EDQISFRSDSSMFE-GIEDYSHQIAGMIGLRNVSNFIQAG--IRTILDIGCGYGSFGAHL 229
            ++I+F    + F  G + Y   +A M+   N  N +  G  +RT LD+GCG  SFG +L
Sbjct: 174 GEKINFPGGGTHFHYGADKYIASMANMLNFPN--NVLNNGGRLRTFLDVGCGVASFGGYL 233

Query: 230 FSKQLLTMCMANYETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWD 289
            + +++TM +A  +   +Q+Q  LERG+PA LG   +K+LPYPS SF++ HC++C IDW 
Sbjct: 234 LASEIMTMSLAPNDVHQNQIQFALERGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWL 293

Query: 290 LQDGIYLIEVDRVLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQL 349
            +DGI L+E+DRVLRPGGYF ++SP    E++  ++ + + W  +      +CW + ++ 
Sbjct: 294 QRDGILLLELDRVLRPGGYFAYSSP----EAYAQDEEDLRIWREMSALVGRMCWTIAAKR 353

Query: 350 EKTVVWKKTSESSCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCI-------GGTKSSRW 409
            +TV+W+K   + CY  R+ G+ P LC      ++ Y   +E CI         TK S  
Sbjct: 354 NQTVIWQKPLTNDCYLGREPGTQPPLCNSDSDPDAVYGVNMEACITQYSDHDHKTKGSGL 413

Query: 410 IPVEERTWPSRANLNKSELAVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPG 469
            P     WP+R       LA +G   + F +D+  W+  V+ YW L+SP I SD      
Sbjct: 414 AP-----WPARLTSPPPRLADFGYSTDIFEKDTETWRQRVDTYWDLLSPKIQSD------ 473

Query: 470 DDDPLPPYNMLRNVLDMNARFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGV 529
                     +RN++DM A  G F +AL E  KDVWVMN VP  G N L LI DRG +G 
Sbjct: 474 ---------TVRNIMDMKASMGSFAAALKE--KDVWVMNVVPEDGPNTLKLIYDRGLMGA 533

Query: 530 LHDWCEAFPSYPRTYDMVHAEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNL 589
           +H WCEAF +YPRTYD++HA  I+S +  +  C+  D+L E+DR+LRP G+++I D  ++
Sbjct: 534 VHSWCEAFSTYPRTYDLLHAWDIIS-DIKKRGCSAEDLLLEMDRILRPSGFILIRDKQSV 593

Query: 590 IESARTLTTQLKWDA----RVIEIESDNDKSLLVCQK 612
           ++  +     L W+A       E + D+D  +L+ QK
Sbjct: 594 VDLVKKYLKALHWEAVETKTASESDQDSDNVILIVQK 598

BLAST of Moc08g02170 vs. ExPASy TrEMBL
Match: A0A6J1BRS5 (Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111005117 PE=3 SV=1)

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 617/683 (90.34%), Postives = 617/683 (90.34%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC 60
           MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC
Sbjct: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC 60

Query: 61  HENSFLSDSFIAGTLRSRHK---------------------------------------- 120
           HENSFLSDSFIAGTLRSRHK                                        
Sbjct: 61  HENSFLSDSFIAGTLRSRHKLAKQIXNFSIPMIVLVIIFGLFWWSVSISIKSRLHTVRSY 120

Query: 121 -----------------------LKELEFCSQDIENHVPCFRVSDNPGSGYSDRYCAREP 180
                                  LKELEFC QDIENHVPCF VSDNPGSGYSDRYCAREP
Sbjct: 121 DKIQEQVVLYLSXIGELALGPSRLKELEFCXQDIENHVPCFXVSDNPGSGYSDRYCAREP 180

Query: 181 KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD 240
           KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD
Sbjct: 181 KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD 240

Query: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN 300
           SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN
Sbjct: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN 300

Query: 301 YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR 360
           YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR
Sbjct: 301 YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR 360

Query: 361 VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES 420
           VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES
Sbjct: 361 VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES 420

Query: 421 SCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL 480
           SCYR RKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL
Sbjct: 421 SCYRXRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL 480

Query: 481 AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA 540
           AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA
Sbjct: 481 AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA 540

Query: 541 RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH 600
           RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH
Sbjct: 541 RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH 600

Query: 601 AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE 621
           AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE
Sbjct: 601 AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE 660

BLAST of Moc08g02170 vs. ExPASy TrEMBL
Match: A0A6J1HDD0 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462579 PE=3 SV=1)

HSP 1 Score: 1053.1 bits (2722), Expect = 4.4e-304
Identity = 509/683 (74.52%), Postives = 549/683 (80.38%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC 60
           M KPLHRG SG RICEG  DL+  E GD D +  S  SD + R                 
Sbjct: 1   MLKPLHRGVSGTRICEGSRDLNGTEKGDFDAKCSSYESDEMFR----------------- 60

Query: 61  HENSFLSDSFIAGTLRSRHK---------------------------------------- 120
               FLSDSF+ GTLR++HK                                        
Sbjct: 61  ----FLSDSFVLGTLRNQHKLAKQMLSFSIPLIVIVIFFGLFWWSVSISTSSRLHAARSY 120

Query: 121 -----------------------LKELEFCSQDIENHVPCFRVSDNPGSGYSDRYCAREP 180
                                  L+ELEFCSQD ENHVPCF   +NP SGY+DR C  EP
Sbjct: 121 DKIQEQVVLYLSEIGELALGPSRLRELEFCSQDFENHVPCFSARENPDSGYADRSCELEP 180

Query: 181 KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD 240
           KQNCIV PPVKYR+PLRWP GRDVIWFANV++TA+EVLSSGS+TKRMMM+EE+QISFRSD
Sbjct: 181 KQNCIVPPPVKYRVPLRWPIGRDVIWFANVKMTADEVLSSGSMTKRMMMMEEEQISFRSD 240

Query: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN 300
           SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMC+AN
Sbjct: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCVAN 300

Query: 301 YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR 360
           YETSGSQVQLTLERGLPAML +FASKQLPYPS SFDMVHCAQCGIDWDL+DGIYL EVDR
Sbjct: 301 YETSGSQVQLTLERGLPAMLSTFASKQLPYPSFSFDMVHCAQCGIDWDLKDGIYLTEVDR 360

Query: 361 VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES 420
           VLRPGGYFVWTSP+ +A+SFLHNKTN K+WN +RDFTE LCWEMLSQL+KTVVWKKTS+S
Sbjct: 361 VLRPGGYFVWTSPIADAQSFLHNKTNLKKWNAVRDFTESLCWEMLSQLDKTVVWKKTSKS 420

Query: 421 SCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL 480
           SCY  RK  SGP LC KGHYVESPYYRPLE+CIGGTKSSRW PVE+RTWPSRANLNKS+L
Sbjct: 421 SCYHSRKPRSGPPLCSKGHYVESPYYRPLENCIGGTKSSRWFPVEQRTWPSRANLNKSKL 480

Query: 481 AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA 540
           AVYGVQ E+FAEDS+KWKMAVNDYWPLMSPLIFSDHPKRPGDDDP PPYNMLRNVLDMNA
Sbjct: 481 AVYGVQREDFAEDSMKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPTPPYNMLRNVLDMNA 540

Query: 541 RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH 600
           +FGGFNSALLESGKDVWVMN VPTTGSNHLPLIVDRGF+GVLHDWCEAFP+YPRTYDMVH
Sbjct: 541 QFGGFNSALLESGKDVWVMNAVPTTGSNHLPLIVDRGFLGVLHDWCEAFPTYPRTYDMVH 600

Query: 601 AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE 621
           A+GILSLEALRHRC+MLD+L+EIDRLLRPEGWVIIHDTTNLIESAR LTTQLKWDARVIE
Sbjct: 601 ADGILSLEALRHRCSMLDLLSEIDRLLRPEGWVIIHDTTNLIESARMLTTQLKWDARVIE 660

BLAST of Moc08g02170 vs. ExPASy TrEMBL
Match: A0A6J1K8J6 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111492106 PE=3 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 6.4e-303
Identity = 505/683 (73.94%), Postives = 547/683 (80.09%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFSNHSDILCRFPFGLSSPKTTPSKYGC 60
           M KPLHRG SG RICEG HDL+  E GD D +  S  SD + R                 
Sbjct: 1   MLKPLHRGVSGTRICEGSHDLNGTEKGDFDVKGSSYESDEMFR----------------- 60

Query: 61  HENSFLSDSFIAGTLRSRHK---------------------------------------- 120
               FLSDSF+ GTLR++HK                                        
Sbjct: 61  ----FLSDSFVLGTLRNQHKLAKQMLNFSIPLIVLVIFFGLFWWSVSISTSSRLHAARSY 120

Query: 121 -----------------------LKELEFCSQDIENHVPCFRVSDNPGSGYSDRYCAREP 180
                                  L+ELEFCSQD ENHVPCF   +NP SG +DR C  EP
Sbjct: 121 DKIQEQVVLYLSEIGELALGPSRLRELEFCSQDFENHVPCFSARENPDSGSADRSCEVEP 180

Query: 181 KQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSD 240
           KQNCIV PPVKYR+PLRWP GRDVIWFANV++TA+EVLSSGS+TKRMMM+EE+QISFRSD
Sbjct: 181 KQNCIVPPPVKYRVPLRWPIGRDVIWFANVKMTADEVLSSGSMTKRMMMMEEEQISFRSD 240

Query: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMAN 300
           SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMC+AN
Sbjct: 241 SSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCIAN 300

Query: 301 YETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDR 360
           YETSGSQVQLTLERGLPAML SFASKQLPYPS SFDMVHCAQCGIDWDL+DGIYL EVDR
Sbjct: 301 YETSGSQVQLTLERGLPAMLSSFASKQLPYPSFSFDMVHCAQCGIDWDLKDGIYLTEVDR 360

Query: 361 VLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSES 420
           VLRPGGYFVW SP+ +A+SFLHNKTN K+WN +RDFT+ LCWEMLSQL+KTVVWKKTS+S
Sbjct: 361 VLRPGGYFVWMSPIADAQSFLHNKTNLKKWNAVRDFTKSLCWEMLSQLDKTVVWKKTSKS 420

Query: 421 SCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSEL 480
           SCY  RK  SGP LC KGHYVESPYYRPLE+CIGGTKSSRW PVE+RTWPSRANLNKS+L
Sbjct: 421 SCYHSRKPSSGPPLCNKGHYVESPYYRPLENCIGGTKSSRWFPVEQRTWPSRANLNKSKL 480

Query: 481 AVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNA 540
           AVYGVQWE+FAEDS+KWKMAVNDYWPLMSPLIFSDHPKRPG+DDP PPYNMLRNVLDMNA
Sbjct: 481 AVYGVQWEDFAEDSMKWKMAVNDYWPLMSPLIFSDHPKRPGEDDPTPPYNMLRNVLDMNA 540

Query: 541 RFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVH 600
           +FGGFNSALLESGKDVWVMN VPTTGSNHLPLIVDRGF+GVLHDWCEAFP+YPRTYDMVH
Sbjct: 541 QFGGFNSALLESGKDVWVMNAVPTTGSNHLPLIVDRGFLGVLHDWCEAFPTYPRTYDMVH 600

Query: 601 AEGILSLEALRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIE 621
           A+ ILSLEA+RHRC+MLD+L+EIDRLLRPEGWVIIHD TNLIESAR LTTQLKWDARVIE
Sbjct: 601 ADSILSLEAVRHRCSMLDLLSEIDRLLRPEGWVIIHDATNLIESARRLTTQLKWDARVIE 660

BLAST of Moc08g02170 vs. ExPASy TrEMBL
Match: A0A067H089 (Methyltransferase OS=Citrus sinensis OX=2711 GN=CISIN_1g005417mg PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 3.6e-269
Identity = 457/697 (65.57%), Postives = 530/697 (76.04%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHD------LDRAENGDLDDRDFSNHSDILCRFPFGLSS--PK 60
           MS+PL RG SG RI   GHD       +R E  DL+    ++ S +  RFPF   S    
Sbjct: 1   MSRPLLRGVSGMRISGNGHDPWDLEMKERNEKEDLEKDHSTDQSYLALRFPFRFLSHDNN 60

Query: 61  TTPSKYGCHENSFLSDSFIAGTLRSRH--------------------------------- 120
           T+PSKYG  EN F SD F +GT RSR                                  
Sbjct: 61  TSPSKYGISENGFTSDIFSSGTPRSRQNFTMLFLKFSLIAIVFLALTGSFWWTISISTSS 120

Query: 121 ------------------------------KLKELEFCSQDIENHVPCFRVSDNPGSGYS 180
                                         + K+LEFCS+D EN+VPCF  S N   GYS
Sbjct: 121 RGHIYHGYRRLQEQLVSDLWDIGEISLGSSRSKDLEFCSEDFENYVPCFNESRNLALGYS 180

Query: 181 -----DRYCAREPKQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRM 240
                DR+C +E KQ+C+VLPPVKYRIPLRWP+GRDVIW +NV+ITA+EVLSSGSLTKRM
Sbjct: 181 NGDEVDRHCGQELKQHCLVLPPVKYRIPLRWPTGRDVIWVSNVKITAQEVLSSGSLTKRM 240

Query: 241 MMLEEDQISFRSDSSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAH 300
           MMLEE+QISFRS S +F+G+EDYSHQIA MIGLRN SNFI AG+RTILDIGCGYGSFGAH
Sbjct: 241 MMLEEEQISFRSASLIFDGVEDYSHQIAEMIGLRNESNFILAGVRTILDIGCGYGSFGAH 300

Query: 301 LFSKQLLTMCMANYETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDW 360
           LFSK+LLTMC+ANYE SGSQVQLTLERGLPAM+GSFASKQLPYPSLSFDM+HCA+CG+DW
Sbjct: 301 LFSKELLTMCIANYEASGSQVQLTLERGLPAMIGSFASKQLPYPSLSFDMLHCARCGVDW 360

Query: 361 DLQDGIYLIEVDRVLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQ 420
           D +DGI L+EVDRVL+PGGYFVWTSPLTN ++FL NK NQKRWN +RDF E LCWE++SQ
Sbjct: 361 DQKDGILLLEVDRVLKPGGYFVWTSPLTNPQAFLRNKENQKRWNFVRDFVENLCWELVSQ 420

Query: 421 LEKTVVWKKTSESSCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEE- 480
            ++TVVWKKTS++SCY  RK GSGPS+C KG+ VESPYYRPL+ CIGGT++ RWIP+EE 
Sbjct: 421 QDETVVWKKTSKASCYSSRKPGSGPSICSKGNDVESPYYRPLQPCIGGTRNRRWIPIEER 480

Query: 481 RTWPSRANLNKSELAVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPL 540
           R WPSRANLNK+ELAVYGV  EEFAED+  WK AV ++W L+SPLIFSDHPKRPGD+DP 
Sbjct: 481 RNWPSRANLNKNELAVYGVHPEEFAEDTENWKTAVGNFWSLLSPLIFSDHPKRPGDEDPS 540

Query: 541 PPYNMLRNVLDMNARFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWC 600
           PPYNM+RNVLDMNA FGGFNSALLE GK VWVMN VPT G+NHLP+I+DRGF+GVLHDWC
Sbjct: 541 PPYNMVRNVLDMNAHFGGFNSALLEKGKSVWVMNVVPTIGTNHLPMILDRGFVGVLHDWC 600

Query: 601 EAFPSYPRTYDMVHAEGILSLEA-LRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESA 620
           EAFP+YPRTYD+VHAEG+LSLE+  RHRC+ LD+ TEIDR+LRPEGWVII DT  LIESA
Sbjct: 601 EAFPTYPRTYDLVHAEGLLSLESGHRHRCSTLDIFTEIDRILRPEGWVIIRDTARLIESA 660

BLAST of Moc08g02170 vs. ExPASy TrEMBL
Match: V4T270 (Methyltransferase OS=Citrus clementina OX=85681 GN=CICLE_v10000463mg PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.8e-268
Identity = 456/697 (65.42%), Postives = 530/697 (76.04%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHD------LDRAENGDLDDRDFSNHSDILCRFPFGLSS--PK 60
           MS+PL RG SG RI   GHD       +R E  DL+    ++ S +  RFPF   S    
Sbjct: 1   MSRPLLRGVSGMRISGNGHDPWDLEMKERNEKEDLEKDHSTDQSYLALRFPFRFLSHDNN 60

Query: 61  TTPSKYGCHENSFLSDSFIAGTLRSRH--------------------------------- 120
           T+PSKYG  EN F SD F +GT RSR                                  
Sbjct: 61  TSPSKYGISENGFTSDIFSSGTPRSRQNFTMLFLKFSLIAIVFLALTGSFWWTISISTSS 120

Query: 121 ------------------------------KLKELEFCSQDIENHVPCFRVSDNPGSGYS 180
                                         + K+LEFCS+D EN+VPCF  S N   GYS
Sbjct: 121 RGHIYHGYRRLQEQLVSDLWDIGEISLGSSRSKDLEFCSEDFENYVPCFNESRNLALGYS 180

Query: 181 -----DRYCAREPKQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRM 240
                DR+C +E KQ+C+VLPPVKYRIPLRWP+GRDVIW +NV+ITA+EVLSSGSLTKRM
Sbjct: 181 NGDEVDRHCGQELKQHCLVLPPVKYRIPLRWPTGRDVIWVSNVKITAQEVLSSGSLTKRM 240

Query: 241 MMLEEDQISFRSDSSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAH 300
           MMLEE+QISFRS S +F+G+EDYSHQIA MIGLRN SNFI AG+RTILDIGCGYGSFGAH
Sbjct: 241 MMLEEEQISFRSASLIFDGVEDYSHQIAEMIGLRNESNFILAGVRTILDIGCGYGSFGAH 300

Query: 301 LFSKQLLTMCMANYETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDW 360
           LFSK+LLTMC+ANYE SGSQVQLTLERGLPAM+GSFASKQLPYPSLSFDM+HCA+CG+DW
Sbjct: 301 LFSKELLTMCIANYEASGSQVQLTLERGLPAMIGSFASKQLPYPSLSFDMLHCARCGVDW 360

Query: 361 DLQDGIYLIEVDRVLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQ 420
           D +DGI L+EVDRVL+PGGYFVWTSPLTN ++FL NK NQKRWN +RDF E LCWE++SQ
Sbjct: 361 DQKDGILLLEVDRVLKPGGYFVWTSPLTNPQAFLRNKENQKRWNFVRDFVENLCWELVSQ 420

Query: 421 LEKTVVWKKTSESSCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEE- 480
            ++TVVWKKTS++SCY  RK GSGPS+C KG+ VESPYYRPL+ CIGGT++ RWIP+EE 
Sbjct: 421 QDETVVWKKTSKASCYSSRKPGSGPSICSKGNDVESPYYRPLQPCIGGTRNRRWIPIEER 480

Query: 481 RTWPSRANLNKSELAVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPL 540
           R WPSRANLNK+ELAVYGV  EEFAED+  WK AV ++W L+SPLIFSDHPKRPGD+DP 
Sbjct: 481 RNWPSRANLNKNELAVYGVHPEEFAEDTENWKTAVGNFWSLLSPLIFSDHPKRPGDEDPS 540

Query: 541 PPYNMLRNVLDMNARFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWC 600
           PPYNM+RNVLDMNA FGGFNSALLE GK VWVMN VPT G+N+LP+I+DRGF+GVLHDWC
Sbjct: 541 PPYNMVRNVLDMNAHFGGFNSALLEKGKSVWVMNVVPTIGTNYLPMILDRGFVGVLHDWC 600

Query: 601 EAFPSYPRTYDMVHAEGILSLEA-LRHRCTMLDMLTEIDRLLRPEGWVIIHDTTNLIESA 620
           EAFP+YPRTYD+VHAEG+LSLE+  RHRC+ LD+ TEIDR+LRPEGWVII DT  LIESA
Sbjct: 601 EAFPTYPRTYDLVHAEGLLSLESGHRHRCSTLDIFTEIDRILRPEGWVIIRDTARLIESA 660

BLAST of Moc08g02170 vs. TAIR 10
Match: AT1G78240.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 825.9 bits (2132), Expect = 2.2e-239
Identity = 400/688 (58.14%), Postives = 492/688 (71.51%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFS-NHSDILCRFPFGLSSPKTTPSKY- 60
           MS PL RG SG R+ +   DL  ++  D  +R  S  ++++  RFPFG      + SK+ 
Sbjct: 1   MSMPLQRGISGVRVSDSSDDLRDSQMKDKTERARSTENNNLTLRFPFGFLFSNQSSSKHG 60

Query: 61  GCHENSFLSDSFIAGTLRSRHKL------------------------------------- 120
           G  EN F +D + A   RSRH+L                                     
Sbjct: 61  GGGENGFSADPYSA---RSRHRLMLLFLKISLVLIVVIALAGSFWWTISISTSSRGHVYH 120

Query: 121 --------------------------KELEFCSQDIENHVPCFRVSDNPGSGYS-----D 180
                                     KELE+C+ + EN VPCF VS+N   GYS     D
Sbjct: 121 NYRRLQEQLVSDLWDIGEISLGPNRWKELEYCNIESENFVPCFNVSENLALGYSNGDEND 180

Query: 181 RYCAREPKQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEED 240
           R+C    KQ C+ LPPVKYR+PLRWP+G+D+IW +NV+ITA+EV+SSGS+TKRMMM+E+D
Sbjct: 181 RFCGPGSKQECLELPPVKYRVPLRWPTGKDIIWHSNVKITAQEVVSSGSITKRMMMMEDD 240

Query: 241 QISFRSDSSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQL 300
           QISFRS S M + +EDYSHQIA MIG++   NFI+AG+RTILDIGCGYGSFGAHL SKQ+
Sbjct: 241 QISFRSASPMSDEVEDYSHQIAEMIGIKK-DNFIEAGVRTILDIGCGYGSFGAHLLSKQI 300

Query: 301 LTMCMANYETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGI 360
           LTMC+ANYE SGSQVQLTLERGLPAM+GSF SKQLPYPSLSFDM+HC +CGIDWD +DG+
Sbjct: 301 LTMCIANYEASGSQVQLTLERGLPAMIGSFISKQLPYPSLSFDMLHCLRCGIDWDQKDGL 360

Query: 361 YLIEVDRVLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVV 420
            L+E+DRVL+PGGYFVWTSPLTN      NK + KRWN + DF E +CW +L+Q ++TVV
Sbjct: 361 LLVEIDRVLKPGGYFVWTSPLTNP----RNKDHLKRWNFVHDFAESICWTLLNQQDETVV 420

Query: 421 WKKTSESSCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERT-WPSR 480
           WKKT  + CY  RK G GPS+C KGH VESPYYRPL+ CIGGT+S RWIP+E RT WPSR
Sbjct: 421 WKKTINTKCYSSRKPGVGPSVCTKGHDVESPYYRPLQMCIGGTRSRRWIPIEGRTRWPSR 480

Query: 481 ANLNKSELAVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNML 540
           +N+NK+EL++YG+  E   ED+  WK+ V +YW L+SPLIFSDHPKRPGD+DP PPYNML
Sbjct: 481 SNMNKTELSLYGLHPEVLGEDAENWKITVREYWSLLSPLIFSDHPKRPGDEDPSPPYNML 540

Query: 541 RNVLDMNARFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSY 600
           RNVLDMNA+FGG NSALLE+ K VWVMN VPT G NHLP+I+DRGF+GVLH+WCE FP+Y
Sbjct: 541 RNVLDMNAQFGGLNSALLEARKSVWVMNVVPTAGPNHLPMILDRGFVGVLHNWCEPFPTY 600

Query: 601 PRTYDMVHAEGILSLEALRHR--CTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTT 616
           PRTYD+VHA+ +LSL+  + R  C ++D+ TEIDRLLRPEGWVII DT  L+E AR   T
Sbjct: 601 PRTYDLVHADNLLSLQTSQPRKTCLLIDIFTEIDRLLRPEGWVIIRDTAQLVEKARETIT 660

BLAST of Moc08g02170 vs. TAIR 10
Match: AT1G78240.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 825.9 bits (2132), Expect = 2.2e-239
Identity = 400/688 (58.14%), Postives = 492/688 (71.51%), Query Frame = 0

Query: 1   MSKPLHRGASGARICEGGHDLDRAENGDLDDRDFS-NHSDILCRFPFGLSSPKTTPSKY- 60
           MS PL RG SG R+ +   DL  ++  D  +R  S  ++++  RFPFG      + SK+ 
Sbjct: 1   MSMPLQRGISGVRVSDSSDDLRDSQMKDKTERARSTENNNLTLRFPFGFLFSNQSSSKHG 60

Query: 61  GCHENSFLSDSFIAGTLRSRHKL------------------------------------- 120
           G  EN F +D + A   RSRH+L                                     
Sbjct: 61  GGGENGFSADPYSA---RSRHRLMLLFLKISLVLIVVIALAGSFWWTISISTSSRGHVYH 120

Query: 121 --------------------------KELEFCSQDIENHVPCFRVSDNPGSGYS-----D 180
                                     KELE+C+ + EN VPCF VS+N   GYS     D
Sbjct: 121 NYRRLQEQLVSDLWDIGEISLGPNRWKELEYCNIESENFVPCFNVSENLALGYSNGDEND 180

Query: 181 RYCAREPKQNCIVLPPVKYRIPLRWPSGRDVIWFANVRITAEEVLSSGSLTKRMMMLEED 240
           R+C    KQ C+ LPPVKYR+PLRWP+G+D+IW +NV+ITA+EV+SSGS+TKRMMM+E+D
Sbjct: 181 RFCGPGSKQECLELPPVKYRVPLRWPTGKDIIWHSNVKITAQEVVSSGSITKRMMMMEDD 240

Query: 241 QISFRSDSSMFEGIEDYSHQIAGMIGLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQL 300
           QISFRS S M + +EDYSHQIA MIG++   NFI+AG+RTILDIGCGYGSFGAHL SKQ+
Sbjct: 241 QISFRSASPMSDEVEDYSHQIAEMIGIKK-DNFIEAGVRTILDIGCGYGSFGAHLLSKQI 300

Query: 301 LTMCMANYETSGSQVQLTLERGLPAMLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGI 360
           LTMC+ANYE SGSQVQLTLERGLPAM+GSF SKQLPYPSLSFDM+HC +CGIDWD +DG+
Sbjct: 301 LTMCIANYEASGSQVQLTLERGLPAMIGSFISKQLPYPSLSFDMLHCLRCGIDWDQKDGL 360

Query: 361 YLIEVDRVLRPGGYFVWTSPLTNAESFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVV 420
            L+E+DRVL+PGGYFVWTSPLTN      NK + KRWN + DF E +CW +L+Q ++TVV
Sbjct: 361 LLVEIDRVLKPGGYFVWTSPLTNP----RNKDHLKRWNFVHDFAESICWTLLNQQDETVV 420

Query: 421 WKKTSESSCYRLRKSGSGPSLCGKGHYVESPYYRPLEDCIGGTKSSRWIPVEERT-WPSR 480
           WKKT  + CY  RK G GPS+C KGH VESPYYRPL+ CIGGT+S RWIP+E RT WPSR
Sbjct: 421 WKKTINTKCYSSRKPGVGPSVCTKGHDVESPYYRPLQMCIGGTRSRRWIPIEGRTRWPSR 480

Query: 481 ANLNKSELAVYGVQWEEFAEDSIKWKMAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNML 540
           +N+NK+EL++YG+  E   ED+  WK+ V +YW L+SPLIFSDHPKRPGD+DP PPYNML
Sbjct: 481 SNMNKTELSLYGLHPEVLGEDAENWKITVREYWSLLSPLIFSDHPKRPGDEDPSPPYNML 540

Query: 541 RNVLDMNARFGGFNSALLESGKDVWVMNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSY 600
           RNVLDMNA+FGG NSALLE+ K VWVMN VPT G NHLP+I+DRGF+GVLH+WCE FP+Y
Sbjct: 541 RNVLDMNAQFGGLNSALLEARKSVWVMNVVPTAGPNHLPMILDRGFVGVLHNWCEPFPTY 600

Query: 601 PRTYDMVHAEGILSLEALRHR--CTMLDMLTEIDRLLRPEGWVIIHDTTNLIESARTLTT 616
           PRTYD+VHA+ +LSL+  + R  C ++D+ TEIDRLLRPEGWVII DT  L+E AR   T
Sbjct: 601 PRTYDLVHADNLLSLQTSQPRKTCLLIDIFTEIDRLLRPEGWVIIRDTAQLVEKARETIT 660

BLAST of Moc08g02170 vs. TAIR 10
Match: AT1G13860.4 (QUASIMODO2 LIKE 1 )

HSP 1 Score: 636.3 bits (1640), Expect = 2.5e-182
Identity = 294/540 (54.44%), Postives = 397/540 (73.52%), Query Frame = 0

Query: 79  HKLKELEFCSQDIENHVPCFRVSDNPGSGYSDRYC--AREPKQNCIVLPPVKYRIPLRWP 138
           ++LKE   C ++ +N+VPC+ V++      SDR C  ARE ++ C+V PP  Y+IPLRWP
Sbjct: 76  NRLKEFPLCGKERDNYVPCYNVTE------SDRNCEFARE-EERCLVRPPRDYKIPLRWP 135

Query: 139 SGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSDSSM-FEGIEDYSHQIAGMI 198
            GRD+IW  NV+IT ++ LSSG++TKR+M+LEE+QI+F SD  + F+G++DY+ QIA MI
Sbjct: 136 VGRDIIWTGNVKITKDQFLSSGTMTKRLMLLEENQITFHSDDGLIFDGVKDYAFQIAEMI 195

Query: 199 GLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMANYETSGSQVQLTLERGLPA 258
           GL + + F QAGIRT+LDIGCG+GSFGAHL S  ++ +C+A YETSGSQVQL LERGLPA
Sbjct: 196 GLGSDTEFPQAGIRTVLDIGCGFGSFGAHLVSLNVMPICIAEYETSGSQVQLALERGLPA 255

Query: 259 MLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDRVLRPGGYFVWTSPLTNAE 318
           M+G+F SKQLPYP+LSFDMVHCAQCGI WD++D + L+EVDRVL+PGGYFV TSP + A+
Sbjct: 256 MIGNFFSKQLPYPALSFDMVHCAQCGITWDIKDAMLLLEVDRVLKPGGYFVLTSPTSKAQ 315

Query: 319 SFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSESSCYRLRKSGSGPSLCGKG 378
               +         + + ++ +CW +  Q ++T +W+KT++ +CY  R   S P +C   
Sbjct: 316 GNSPDTKKTSISTRVDELSKKICWSLSGQQDETFLWQKTADPNCYSSRSQASIP-VCKDD 375

Query: 379 HYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSELAVYGVQWEEFAEDSIKWK 438
             V  PYY PL  CI GTKS RWIP++ R+  S  +L  SEL ++G++ EEF ED   W+
Sbjct: 376 DSV--PYYHPLVPCISGTKSKRWIPIQNRSRASGTSL--SELEIHGIKPEEFDEDIQVWR 435

Query: 439 MAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNARFGGFNSALLESGKDVWV 498
            A+ +YW L++PLIFSDHPKRPGD+DP+PP+ M+RN +DMNAR+G  N ALL  GK VWV
Sbjct: 436 SALKNYWSLLTPLIFSDHPKRPGDEDPVPPFYMIRNAMDMNARYGNLNQALLNQGKSVWV 495

Query: 499 MNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVHAEGILSLEALRHRCTMLD 558
           MN VP    N LP+I+DRGF G LHDWCE FP+YPRTYDM+HA  +L+      RC+++D
Sbjct: 496 MNVVPVKARNTLPIILDRGFTGALHDWCEPFPTYPRTYDMLHANELLT-HLSSERCSLMD 555

Query: 559 MLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIEIESDNDKSLLVCQKPFLK 616
           +  E+DR+LRPEGWV++ D   +IE ARTL  +++W+ARVI+I+  +D+ LLVCQKP LK
Sbjct: 556 LFLEMDRILRPEGWVVLSDKLGVIEMARTLAARVRWEARVIDIQDGSDQRLLVCQKPLLK 602

BLAST of Moc08g02170 vs. TAIR 10
Match: AT1G13860.1 (QUASIMODO2 LIKE 1 )

HSP 1 Score: 636.3 bits (1640), Expect = 2.5e-182
Identity = 294/540 (54.44%), Postives = 397/540 (73.52%), Query Frame = 0

Query: 79  HKLKELEFCSQDIENHVPCFRVSDNPGSGYSDRYC--AREPKQNCIVLPPVKYRIPLRWP 138
           ++LKE   C ++ +N+VPC+ V++      SDR C  ARE ++ C+V PP  Y+IPLRWP
Sbjct: 76  NRLKEFPLCGKERDNYVPCYNVTE------SDRNCEFARE-EERCLVRPPRDYKIPLRWP 135

Query: 139 SGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSDSSM-FEGIEDYSHQIAGMI 198
            GRD+IW  NV+IT ++ LSSG++TKR+M+LEE+QI+F SD  + F+G++DY+ QIA MI
Sbjct: 136 VGRDIIWTGNVKITKDQFLSSGTMTKRLMLLEENQITFHSDDGLIFDGVKDYAFQIAEMI 195

Query: 199 GLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMANYETSGSQVQLTLERGLPA 258
           GL + + F QAGIRT+LDIGCG+GSFGAHL S  ++ +C+A YETSGSQVQL LERGLPA
Sbjct: 196 GLGSDTEFPQAGIRTVLDIGCGFGSFGAHLVSLNVMPICIAEYETSGSQVQLALERGLPA 255

Query: 259 MLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDRVLRPGGYFVWTSPLTNAE 318
           M+G+F SKQLPYP+LSFDMVHCAQCGI WD++D + L+EVDRVL+PGGYFV TSP + A+
Sbjct: 256 MIGNFFSKQLPYPALSFDMVHCAQCGITWDIKDAMLLLEVDRVLKPGGYFVLTSPTSKAQ 315

Query: 319 SFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSESSCYRLRKSGSGPSLCGKG 378
               +         + + ++ +CW +  Q ++T +W+KT++ +CY  R   S P +C   
Sbjct: 316 GNSPDTKKTSISTRVDELSKKICWSLSGQQDETFLWQKTADPNCYSSRSQASIP-VCKDD 375

Query: 379 HYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSELAVYGVQWEEFAEDSIKWK 438
             V  PYY PL  CI GTKS RWIP++ R+  S  +L  SEL ++G++ EEF ED   W+
Sbjct: 376 DSV--PYYHPLVPCISGTKSKRWIPIQNRSRASGTSL--SELEIHGIKPEEFDEDIQVWR 435

Query: 439 MAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNARFGGFNSALLESGKDVWV 498
            A+ +YW L++PLIFSDHPKRPGD+DP+PP+ M+RN +DMNAR+G  N ALL  GK VWV
Sbjct: 436 SALKNYWSLLTPLIFSDHPKRPGDEDPVPPFYMIRNAMDMNARYGNLNQALLNQGKSVWV 495

Query: 499 MNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVHAEGILSLEALRHRCTMLD 558
           MN VP    N LP+I+DRGF G LHDWCE FP+YPRTYDM+HA  +L+      RC+++D
Sbjct: 496 MNVVPVKARNTLPIILDRGFTGALHDWCEPFPTYPRTYDMLHANELLT-HLSSERCSLMD 555

Query: 559 MLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIEIESDNDKSLLVCQKPFLK 616
           +  E+DR+LRPEGWV++ D   +IE ARTL  +++W+ARVI+I+  +D+ LLVCQKP LK
Sbjct: 556 LFLEMDRILRPEGWVVLSDKLGVIEMARTLAARVRWEARVIDIQDGSDQRLLVCQKPLLK 602

BLAST of Moc08g02170 vs. TAIR 10
Match: AT1G13860.3 (QUASIMODO2 LIKE 1 )

HSP 1 Score: 636.3 bits (1640), Expect = 2.5e-182
Identity = 294/540 (54.44%), Postives = 397/540 (73.52%), Query Frame = 0

Query: 79  HKLKELEFCSQDIENHVPCFRVSDNPGSGYSDRYC--AREPKQNCIVLPPVKYRIPLRWP 138
           ++LKE   C ++ +N+VPC+ V++      SDR C  ARE ++ C+V PP  Y+IPLRWP
Sbjct: 76  NRLKEFPLCGKERDNYVPCYNVTE------SDRNCEFARE-EERCLVRPPRDYKIPLRWP 135

Query: 139 SGRDVIWFANVRITAEEVLSSGSLTKRMMMLEEDQISFRSDSSM-FEGIEDYSHQIAGMI 198
            GRD+IW  NV+IT ++ LSSG++TKR+M+LEE+QI+F SD  + F+G++DY+ QIA MI
Sbjct: 136 VGRDIIWTGNVKITKDQFLSSGTMTKRLMLLEENQITFHSDDGLIFDGVKDYAFQIAEMI 195

Query: 199 GLRNVSNFIQAGIRTILDIGCGYGSFGAHLFSKQLLTMCMANYETSGSQVQLTLERGLPA 258
           GL + + F QAGIRT+LDIGCG+GSFGAHL S  ++ +C+A YETSGSQVQL LERGLPA
Sbjct: 196 GLGSDTEFPQAGIRTVLDIGCGFGSFGAHLVSLNVMPICIAEYETSGSQVQLALERGLPA 255

Query: 259 MLGSFASKQLPYPSLSFDMVHCAQCGIDWDLQDGIYLIEVDRVLRPGGYFVWTSPLTNAE 318
           M+G+F SKQLPYP+LSFDMVHCAQCGI WD++D + L+EVDRVL+PGGYFV TSP + A+
Sbjct: 256 MIGNFFSKQLPYPALSFDMVHCAQCGITWDIKDAMLLLEVDRVLKPGGYFVLTSPTSKAQ 315

Query: 319 SFLHNKTNQKRWNTIRDFTEGLCWEMLSQLEKTVVWKKTSESSCYRLRKSGSGPSLCGKG 378
               +         + + ++ +CW +  Q ++T +W+KT++ +CY  R   S P +C   
Sbjct: 316 GNSPDTKKTSISTRVDELSKKICWSLSGQQDETFLWQKTADPNCYSSRSQASIP-VCKDD 375

Query: 379 HYVESPYYRPLEDCIGGTKSSRWIPVEERTWPSRANLNKSELAVYGVQWEEFAEDSIKWK 438
             V  PYY PL  CI GTKS RWIP++ R+  S  +L  SEL ++G++ EEF ED   W+
Sbjct: 376 DSV--PYYHPLVPCISGTKSKRWIPIQNRSRASGTSL--SELEIHGIKPEEFDEDIQVWR 435

Query: 439 MAVNDYWPLMSPLIFSDHPKRPGDDDPLPPYNMLRNVLDMNARFGGFNSALLESGKDVWV 498
            A+ +YW L++PLIFSDHPKRPGD+DP+PP+ M+RN +DMNAR+G  N ALL  GK VWV
Sbjct: 436 SALKNYWSLLTPLIFSDHPKRPGDEDPVPPFYMIRNAMDMNARYGNLNQALLNQGKSVWV 495

Query: 499 MNTVPTTGSNHLPLIVDRGFIGVLHDWCEAFPSYPRTYDMVHAEGILSLEALRHRCTMLD 558
           MN VP    N LP+I+DRGF G LHDWCE FP+YPRTYDM+HA  +L+      RC+++D
Sbjct: 496 MNVVPVKARNTLPIILDRGFTGALHDWCEPFPTYPRTYDMLHANELLT-HLSSERCSLMD 555

Query: 559 MLTEIDRLLRPEGWVIIHDTTNLIESARTLTTQLKWDARVIEIESDNDKSLLVCQKPFLK 616
           +  E+DR+LRPEGWV++ D   +IE ARTL  +++W+ARVI+I+  +D+ LLVCQKP LK
Sbjct: 556 LFLEMDRILRPEGWVVLSDKLGVIEMARTLAARVRWEARVIDIQDGSDQRLLVCQKPLLK 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132200.10.0e+0090.34probable pectin methyltransferase QUA2 [Momordica charantia][more]
XP_023546073.16.4e-30574.67probable pectin methyltransferase QUA2 [Cucurbita pepo subsp. pepo][more]
XP_022961962.19.2e-30474.52probable pectin methyltransferase QUA2 [Cucurbita moschata] >XP_022961963.1 prob... [more]
XP_022997075.11.3e-30273.94probable pectin methyltransferase QUA2 [Cucurbita maxima][more]
KAG6598691.13.5e-29587.25putative pectin methyltransferase QUA2, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
Q9C9Q83.1e-23858.14Probable pectin methyltransferase QUA2 OS=Arabidopsis thaliana OX=3702 GN=QUA2 P... [more]
Q8GYW93.5e-18154.44Probable methyltransferase PMT4 OS=Arabidopsis thaliana OX=3702 GN=At1g13860 PE=... [more]
Q3EC772.0e-17652.95Probable methyltransferase PMT5 OS=Arabidopsis thaliana OX=3702 GN=At2g03480 PE=... [more]
Q8VZV75.5e-10238.26Probable methyltransferase PMT9 OS=Arabidopsis thaliana OX=3702 GN=At5g14430 PE=... [more]
Q8H1181.5e-9936.75Probable methyltransferase PMT1 OS=Arabidopsis thaliana OX=3702 GN=At3g23300 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1BRS50.0e+0090.34Methyltransferase OS=Momordica charantia OX=3673 GN=LOC111005117 PE=3 SV=1[more]
A0A6J1HDD04.4e-30474.52Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462579 PE=3 SV=1[more]
A0A6J1K8J66.4e-30373.94Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111492106 PE=3 SV=1[more]
A0A067H0893.6e-26965.57Methyltransferase OS=Citrus sinensis OX=2711 GN=CISIN_1g005417mg PE=3 SV=1[more]
V4T2701.8e-26865.42Methyltransferase OS=Citrus clementina OX=85681 GN=CICLE_v10000463mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G78240.12.2e-23958.14S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G78240.22.2e-23958.14S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G13860.42.5e-18254.44QUASIMODO2 LIKE 1 [more]
AT1G13860.12.5e-18254.44QUASIMODO2 LIKE 1 [more]
AT1G13860.32.5e-18254.44QUASIMODO2 LIKE 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 108..611
e-value: 9.8E-139
score: 463.2
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePANTHERPTHR10108SAM-DEPENDENT METHYLTRANSFERASEcoord: 66..616
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 175..357
e-value: 1.3E-12
score: 49.4
NoneNo IPR availablePANTHERPTHR10108:SF899PECTIN METHYLTRANSFERASE QUA2-RELATEDcoord: 66..616
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 471..585
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 205..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g02170.1Moc08g02170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0048731 system development
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity