Moc06g40130 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g40130
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Gluconokinase
Location	chr6: 31356538 .. 31357749 (-)
RNA-Seq Expression	Moc06g40130
Synteny	Moc06g40130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCGAGCATCTGAATTCCAAAGGTATGAATATTATTCATCAGTTCTCTCTTAATCTTTTTTCAAATTCATTCATCATTTTTGTTACCGATCTTCGTTTGCTTCGATTTCAATCAAGGCATGGCGATCATCGTGCTTATGGGCGTCTGTGGCTCCGGAAAGTCGTAAGTCCGTTCTTCTTCTCTCTTTAATAATCTACGCCTCGTTGATTCTTCTGCGACCTAGAATTGAATTGGATGATTAAAGTTTGAGTAGCGGAGAAAGTAATATGTTAATCAGATCGATTGAGTTAATTAGTAGATTCGATTTTGAATTTGAAGATCATTTCTGGAATTTGCATTTTTAGTGTGATTCTGTTTTACTTTTCTGTAAGCGAAGGGGAAATTTCCGAAACTGTGATGGATGAGTGAGTTGTGTGTGGTGCAGTACCATTGGAGAGATGCTGGCGAAGGCGATGAACTTGAACTTTCTTGATGCCGATGATTTCCACCCCAATTCTAACAAGGGTAGATAAATCACCAACTCTGTTTTTTTGCTTGTTTACTTCAATATAATTTTGATCATTTTGCCAAAATCACTCCCATGATTGGCATTGGTGTACAAGTTATATGAAAAAAGATCTAAATCAGCTATATTTTCTTCAACTTGAAAAATACTCGAGCAATTTAAATGGCTTTCAAAGTACTTTTTTTTAGAGTGCGTTTATAACTCGAGAATACCATTCTCAAAAGCATTTCAAAATCACTCTACGATCTTCGATCAGAACTTACACTAACACTTTAAAATTATGGGCAGAGAAAATGTCGAAAGGAATCCCTCTTTCGAACGAAGATCGACTGCCATGGCTCGAGAAGGTTCGAGACGCCTTGAAAGAGAGCATAGCTTGTAAGAAAAGTGTGGTTTTGGGGTGTTCAGCTCTTCAAAAACAGTACAGAGAGATTTTGAGATCAGCTGATCCAAATTATGAAATAGGAAGCTGTGTGGTGAAGTTTGTTCTTTTGGATGCTCCACCTGAAGTGATTGCTTACAGATTAGAGAGAAGAGCCAAAGAAGGGAACCATTTCATGTCTTCTACTCTTCTGGAATCTCAATTGGATTTGTTAGAGATTGATGATTCTGAAGGTATTTTAAAGGTTGATGCTACTCTAACTCCTCAAGTTATTGTGACTAATATTCTTAAGGTTGTTTTTGGTTCTCTTTGGTTTTGA

mRNA sequence

ATGGATTCCGAGCATCTGAATTCCAAAGGCATGGCGATCATCGTGCTTATGGGCGTCTGTGGCTCCGGAAAGTCTACCATTGGAGAGATGCTGGCGAAGGCGATGAACTTGAACTTTCTTGATGCCGATGATTTCCACCCCAATTCTAACAAGGAGAAAATGTCGAAAGGAATCCCTCTTTCGAACGAAGATCGACTGCCATGGCTCGAGAAGGTTCGAGACGCCTTGAAAGAGAGCATAGCTTGTAAGAAAAGTGTGGTTTTGGGGTGTTCAGCTCTTCAAAAACAGTACAGAGAGATTTTGAGATCAGCTGATCCAAATTATGAAATAGGAAGCTGTGTGGTGAAGTTTGTTCTTTTGGATGCTCCACCTGAAGTGATTGCTTACAGATTAGAGAGAAGAGCCAAAGAAGGGAACCATTTCATGTCTTCTACTCTTCTGGAATCTCAATTGGATTTGTTAGAGATTGATGATTCTGAAGGTATTTTAAAGGTTGATGCTACTCTAACTCCTCAAGTTATTGTGACTAATATTCTTAAGGTTGTTTTTGGTTCTCTTTGGTTTTGA

Coding sequence (CDS)

ATGGATTCCGAGCATCTGAATTCCAAAGGCATGGCGATCATCGTGCTTATGGGCGTCTGTGGCTCCGGAAAGTCTACCATTGGAGAGATGCTGGCGAAGGCGATGAACTTGAACTTTCTTGATGCCGATGATTTCCACCCCAATTCTAACAAGGAGAAAATGTCGAAAGGAATCCCTCTTTCGAACGAAGATCGACTGCCATGGCTCGAGAAGGTTCGAGACGCCTTGAAAGAGAGCATAGCTTGTAAGAAAAGTGTGGTTTTGGGGTGTTCAGCTCTTCAAAAACAGTACAGAGAGATTTTGAGATCAGCTGATCCAAATTATGAAATAGGAAGCTGTGTGGTGAAGTTTGTTCTTTTGGATGCTCCACCTGAAGTGATTGCTTACAGATTAGAGAGAAGAGCCAAAGAAGGGAACCATTTCATGTCTTCTACTCTTCTGGAATCTCAATTGGATTTGTTAGAGATTGATGATTCTGAAGGTATTTTAAAGGTTGATGCTACTCTAACTCCTCAAGTTATTGTGACTAATATTCTTAAGGTTGTTTTTGGTTCTCTTTGGTTTTGA

Protein sequence

MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIGSCVVKFVLLDAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSLWF

Homology

[Close]

BLAST of Moc06g40130 vs. NCBI nr
Match: XP_022159526.1 (uncharacterized protein LOC111025918 isoform X1 [Momordica charantia])

HSP 1 Score: 372.9 bits (956), Expect = 1.7e-99
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL 60
           MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL
Sbjct: 1   MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL 60

Query: 61  SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIGSCVVKFVLL 120
           SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIGSCVVKFVLL
Sbjct: 61  SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIGSCVVKFVLL 120

Query: 121 DAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILK 180
           DAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILK
Sbjct: 121 DAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILK 180

Query: 181 VVFGSLWF 189
           VVFGSLWF
Sbjct: 181 VVFGSLWF 188

[Close]

BLAST of Moc06g40130 vs. NCBI nr
Match: XP_022159527.1 (uncharacterized protein LOC111025918 isoform X2 [Momordica charantia] >XP_022159528.1 uncharacterized protein LOC111025918 isoform X2 [Momordica charantia])

HSP 1 Score: 316.2 bits (809), Expect = 1.9e-82
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 30  MLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRDALKESIACKKSVVLG 89
           MLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRDALKESIACKKSVVLG
Sbjct: 1   MLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRDALKESIACKKSVVLG 60

Query: 90  CSALQKQYREILRSADPNYEIGSCVVKFVLLDAPPEVIAYRLERRAKEGNHFMSSTLLES 149
           CSALQKQYREILRSADPNYEIGSCVVKFVLLDAPPEVIAYRLERRAKEGNHFMSSTLLES
Sbjct: 61  CSALQKQYREILRSADPNYEIGSCVVKFVLLDAPPEVIAYRLERRAKEGNHFMSSTLLES 120

Query: 150 QLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSLWF 189
           QLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSLWF
Sbjct: 121 QLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSLWF 159

[Close]

BLAST of Moc06g40130 vs. NCBI nr
Match: XP_023532636.1 (gluconokinase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 280.0 bits (715), Expect = 1.5e-71
Identity = 148/189 (78.31%), Postives = 163/189 (86.24%), Query Frame = 0


Query: 1   MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL 60
           MDS    S  MA IVLMGVCGSGKSTIG MLA+AM L FLDADDFHP+SNKEKMSKGIPL
Sbjct: 1   MDSHDPKSNAMA-IVLMGVCGSGKSTIGAMLAEAMGLTFLDADDFHPSSNKEKMSKGIPL 60

Query: 61  SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYE-IG-SCVVKFV 120
           S EDR+PWLEK+RD L+E +  KKSVVLGCSALQKQYR+ILRSADPNYE IG  CVVKFV
Sbjct: 61  SEEDRIPWLEKIRDTLREKMGSKKSVVLGCSALQKQYRDILRSADPNYEGIGIRCVVKFV 120

Query: 121 LLDAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNI 180
           LLDAP EVIA+RLE+RAKEGNHFM STLL SQLDLL+ID SEGILKVDATL+PQ IV+ I
Sbjct: 121 LLDAPAEVIAFRLEKRAKEGNHFMPSTLLNSQLDLLQIDVSEGILKVDATLSPQAIVSRI 180

Query: 181 LKVVFGSLW 188
           +K+V G L+
Sbjct: 181 IKLVRGFLF 188

[Close]

BLAST of Moc06g40130 vs. NCBI nr
Match: XP_038874456.1 (gluconokinase [Benincasa hispida] >XP_038874457.1 gluconokinase [Benincasa hispida])

HSP 1 Score: 278.9 bits (712), Expect = 3.3e-71
Identity = 140/176 (79.55%), Postives = 157/176 (89.20%), Query Frame = 0


Query: 14  IVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVR 73
           IVLMGVCGSGKSTIGEML KA++  FLDADDFHP SNKEKMSKGIPLS+EDR+PWLEK+R
Sbjct: 3   IVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKIR 62

Query: 74  DALKESIACKKSVVLGCSALQKQYREILRSADPNY---EIGSCVVKFVLLDAPPEVIAYR 133
           D L+E+I C++SVVLGCSALQK YREILRS+D NY   E   CVVKFVLLDAP EVIA R
Sbjct: 63  DTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIASR 122

Query: 134 LERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSL 187
           LE+RAKEGNHFM STLL+SQLDLL+IDDSEGI+KVDATLTPQ IV+NI+ +VFGSL
Sbjct: 123 LEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSL 178

[Close]

BLAST of Moc06g40130 vs. NCBI nr
Match: XP_022995367.1 (gluconokinase [Cucurbita maxima])

HSP 1 Score: 275.0 bits (702), Expect = 4.8e-70
Identity = 146/186 (78.49%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 1   MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL 60
           MDS    S  MA IVLMGVCGSGKSTIG MLA+AM L FLDADDFHP+SNKEKMSKGIPL
Sbjct: 1   MDSHDPKSNAMA-IVLMGVCGSGKSTIGAMLAEAMGLTFLDADDFHPSSNKEKMSKGIPL 60

Query: 61  SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYE-IG-SCVVKFV 120
           S+EDR+PWLEK+R  L+E +  K SVVLGCSALQKQYREILRSADPNYE IG SCVVKFV
Sbjct: 61  SDEDRIPWLEKIRGTLREKMGSKNSVVLGCSALQKQYREILRSADPNYEGIGISCVVKFV 120

Query: 121 LLDAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNI 180
           LLDAP EVIA RLE+RAKEGNHFM STLL SQLDLL+ID SEGIL+VDATL+PQ IV++I
Sbjct: 121 LLDAPAEVIALRLEKRAKEGNHFMPSTLLNSQLDLLQIDVSEGILQVDATLSPQAIVSSI 180

Query: 181 LKVVFG 185
           +K+V G
Sbjct: 181 IKLVRG 185

[Close]

BLAST of Moc06g40130 vs. ExPASy Swiss-Prot
Match: Q9SLE0 (Gluconokinase OS=Arabidopsis thaliana OX=3702 GN=At2g16790 PE=2 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 7.4e-50
Identity = 99/177 (55.93%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 13  IIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKV 72
           +I +MGV G+GKSTIG+ML KA++ +FLDADDFH  SN++KM +GI LS+EDR+PWLEK+
Sbjct: 11  VIAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKI 70

Query: 73  RDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIG---SCVVKFVLLDAPPEVIAY 132
           +++L++ +   ++VVL CS+L+KQYREILR +DP+Y+ G   SC V FVLL+   EVIA 
Sbjct: 71  QESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAA 130

Query: 133 RLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSL 187
           RL++RA E  HFM  TLL+SQ DLL+ D+ E I K+   L+P+VIV  IL++V  SL
Sbjct: 131 RLQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTILEMVANSL 187

[Close]

BLAST of Moc06g40130 vs. ExPASy Swiss-Prot
Match: P39208 (Thermosensitive gluconokinase OS=Escherichia coli (strain K12) OX=83333 GN=idnK PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.0e-27
Identity = 69/154 (44.81%), Postives = 96/154 (62.34%), Query Frame = 0


Query: 15  VLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRD 74
           +LMGV GSGK+ IG  +A  ++  F+D DD HP  N +KMS+GIPLS+EDRLPWLE++ D
Sbjct: 7   ILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNIDKMSQGIPLSDEDRLPWLERLND 66

Query: 75  ALKESIACKKSVVLGCSALQKQYREILRSADPNYEIGSCVVKFVLLDAPPEVIAYRLERR 134
           A        ++  + CS+L+KQYR+ILR   P+       V F+ LD   E I  R++RR
Sbjct: 67  ASYSLYKKNETGFIVCSSLKKQYRDILRKGSPH-------VHFLWLDGDYETILARMQRR 126

Query: 135 AKEGNHFMSSTLLESQLDLLEID--DSEGILKVD 167
           A    HFM   LL+SQ + LE    D + I+++D
Sbjct: 127 A---GHFMPVALLKSQFEALERPQADEQDIVRID 150

[Close]

BLAST of Moc06g40130 vs. ExPASy Swiss-Prot
Match: B0BML1 (Probable gluconokinase OS=Xenopus tropicalis OX=8364 GN=idnk PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 74/175 (42.29%), Postives = 101/175 (57.71%), Query Frame = 0


Query: 13  IIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKV 72
           IIV+MGV GSGK+ +G  LAK +  NF DADD+HP  NKEKMS+G PL+++DR PWL ++
Sbjct: 2   IIVIMGVSGSGKTVVGSQLAKKLGWNFYDADDYHPLENKEKMSQGTPLNDQDRHPWLCEL 61

Query: 73  RDALKESIACKKSVVLGCSALQKQYREILRSAD-----PNYEIG---SCVVKFVLLDAPP 132
            + +    A  + VVL CSAL++ YR  L +        NY+     S    FV L    
Sbjct: 62  HEIMMREKALGQHVVLACSALKRAYRSTLLTGSTPHWPENYQENDDLSSDTLFVHLHGSL 121

Query: 133 EVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDS-EGILKVDATLTPQVIVTNI 179
           E+++ RL  R     HFM  TLL+SQ+D LE   + E  + +D      VIV+ I
Sbjct: 122 EILSRRLLERK---GHFMPRTLLDSQIDTLEPPSAPERFIAIDVDKDISVIVSEI 173

[Close]

BLAST of Moc06g40130 vs. ExPASy Swiss-Prot
Match: Q5FQ97 (Gluconokinase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GOX1709 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 70/181 (38.67%), Postives = 103/181 (56.91%), Query Frame = 0


Query: 3   SEHLNSKGM--AIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL 62
           +EH    G+    +V+MGV G+GK+T+   LA  +  +F + D  HP +N EKMS G PL
Sbjct: 2   TEHETQMGLKPRFLVVMGVSGTGKTTVATGLATRLGWHFQEGDALHPPANVEKMSTGQPL 61

Query: 63  SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIGSCVVKFVLL 122
           ++ DR PWL    D L+E +      VL CSAL++ YRE LR  D         ++FV +
Sbjct: 62  TDADRAPWLALCHDWLREQVKAGHGAVLTCSALKRSYREQLRGDD-------LPIEFVHI 121

Query: 123 DAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEI-DDSEGILKVDATLTPQVIVTNIL 181
           D     +A RL+RR  EG HFM ++LL SQL  LE+  D E +++V     P V++  ++
Sbjct: 122 DTSTGELADRLQRR--EG-HFMPASLLPSQLATLEVPGDDEPVIRVSGEKHPDVVLEELI 172

[Close]

BLAST of Moc06g40130 vs. ExPASy Swiss-Prot
Match: Q5T6J7 (Probable gluconokinase OS=Homo sapiens OX=9606 GN=IDNK PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 69/181 (38.12%), Postives = 100/181 (55.25%), Query Frame = 0


Query: 14  IVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVR 73
           +++MGV GSGKST+G +LA  +   F DADD+HP  N+ KM KGIPL+++DR+PWL  + 
Sbjct: 7   LLVMGVSGSGKSTVGALLASELGWKFYDADDYHPEENRRKMGKGIPLNDQDRIPWLCNLH 66

Query: 74  DALKESIACKKSVVLGCSALQKQYREILRSADPNYEI-----------GSCVVKFVLLDA 133
           D L   +A  + VVL CSAL+K YR+IL        +               +  V L  
Sbjct: 67  DILLRDVASGQRVVLACSALKKTYRDILTQGKDGVALKCEESGKEAKQAEMQLLVVHLSG 126

Query: 134 PPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDS-EGILKVDATLTPQVIVTNILKV 183
             EVI+ RL +R  EG HFM   LL+SQ + LE   + E  +++        I+  I++ 
Sbjct: 127 SFEVISGRLLKR--EG-HFMPPELLQSQFETLEPPAAPENFIQISVDKNVSEIIATIMET 184

[Close]

BLAST of Moc06g40130 vs. ExPASy TrEMBL
Match: A0A6J1E468 (Gluconokinase OS=Momordica charantia OX=3673 GN=LOC111025918 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 8.1e-100
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL 60
           MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL
Sbjct: 1   MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL 60

Query: 61  SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIGSCVVKFVLL 120
           SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIGSCVVKFVLL
Sbjct: 61  SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIGSCVVKFVLL 120

Query: 121 DAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILK 180
           DAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILK
Sbjct: 121 DAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILK 180

Query: 181 VVFGSLWF 189
           VVFGSLWF
Sbjct: 181 VVFGSLWF 188

[Close]

BLAST of Moc06g40130 vs. ExPASy TrEMBL
Match: A0A6J1DZ23 (Gluconokinase OS=Momordica charantia OX=3673 GN=LOC111025918 PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 9.0e-83
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 30  MLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRDALKESIACKKSVVLG 89
           MLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRDALKESIACKKSVVLG
Sbjct: 1   MLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVRDALKESIACKKSVVLG 60

Query: 90  CSALQKQYREILRSADPNYEIGSCVVKFVLLDAPPEVIAYRLERRAKEGNHFMSSTLLES 149
           CSALQKQYREILRSADPNYEIGSCVVKFVLLDAPPEVIAYRLERRAKEGNHFMSSTLLES
Sbjct: 61  CSALQKQYREILRSADPNYEIGSCVVKFVLLDAPPEVIAYRLERRAKEGNHFMSSTLLES 120

Query: 150 QLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSLWF 189
           QLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSLWF
Sbjct: 121 QLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSLWF 159

[Close]

BLAST of Moc06g40130 vs. ExPASy TrEMBL
Match: A0A6J1JYN8 (Gluconokinase OS=Cucurbita maxima OX=3661 GN=LOC111490938 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 2.3e-70
Identity = 146/186 (78.49%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 1   MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL 60
           MDS    S  MA IVLMGVCGSGKSTIG MLA+AM L FLDADDFHP+SNKEKMSKGIPL
Sbjct: 1   MDSHDPKSNAMA-IVLMGVCGSGKSTIGAMLAEAMGLTFLDADDFHPSSNKEKMSKGIPL 60

Query: 61  SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYE-IG-SCVVKFV 120
           S+EDR+PWLEK+R  L+E +  K SVVLGCSALQKQYREILRSADPNYE IG SCVVKFV
Sbjct: 61  SDEDRIPWLEKIRGTLREKMGSKNSVVLGCSALQKQYREILRSADPNYEGIGISCVVKFV 120

Query: 121 LLDAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNI 180
           LLDAP EVIA RLE+RAKEGNHFM STLL SQLDLL+ID SEGIL+VDATL+PQ IV++I
Sbjct: 121 LLDAPAEVIALRLEKRAKEGNHFMPSTLLNSQLDLLQIDVSEGILQVDATLSPQAIVSSI 180

Query: 181 LKVVFG 185
           +K+V G
Sbjct: 181 IKLVRG 185

[Close]

BLAST of Moc06g40130 vs. ExPASy TrEMBL
Match: A0A6J1H380 (Gluconokinase OS=Cucurbita moschata OX=3662 GN=LOC111459704 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 8.7e-70
Identity = 145/189 (76.72%), Postives = 161/189 (85.19%), Query Frame = 0


Query: 1   MDSEHLNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPL 60
           MDS    S  MA IVLMGVCGSGKSTIG MLA+AM L FLDADDFHP+SNKEKMSKGIPL
Sbjct: 1   MDSHDPKSNAMA-IVLMGVCGSGKSTIGAMLAEAMGLTFLDADDFHPSSNKEKMSKGIPL 60

Query: 61  SNEDRLPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYE-IG-SCVVKFV 120
           S+EDR+PWLEK+RD L+E +  K SVVLGCSALQKQYR+ILRSADPNYE IG  CVVKFV
Sbjct: 61  SDEDRIPWLEKIRDTLREKMGSKNSVVLGCSALQKQYRDILRSADPNYEGIGIRCVVKFV 120

Query: 121 LLDAPPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNI 180
           LLDAP EVIA RLE+RAKEGNHFM STLL SQLDLL+ID SEGIL+VDAT +PQ IV+ I
Sbjct: 121 LLDAPAEVIALRLEKRAKEGNHFMPSTLLNSQLDLLQIDVSEGILQVDATRSPQAIVSRI 180

Query: 181 LKVVFGSLW 188
           +K+V G L+
Sbjct: 181 IKLVRGFLF 188

[Close]

BLAST of Moc06g40130 vs. ExPASy TrEMBL
Match: A0A0A0KN86 (Gluconokinase OS=Cucumis sativus OX=3659 GN=Csa_5G169050 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.3e-68
Identity = 137/181 (75.69%), Postives = 158/181 (87.29%), Query Frame = 0


Query: 6   LNSKGMAIIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDR 65
           +NS  M +I+LMGVCGSGKSTIGEML KA++  FLDAD FHP SNKEKMSKGIPLS+EDR
Sbjct: 1   MNSNAM-VIILMGVCGSGKSTIGEMLGKALDFTFLDADHFHPISNKEKMSKGIPLSDEDR 60

Query: 66  LPWLEKVRDALKESIACKKSVVLGCSALQKQYREILRSADPNYE-IG---SCVVKFVLLD 125
           +PWLEK+RD L+E+IACK SVVLGCSALQK YREILRS+DPNYE +G    CVVKFVLLD
Sbjct: 61  MPWLEKIRDTLRENIACKSSVVLGCSALQKHYREILRSSDPNYEKVGITMKCVVKFVLLD 120

Query: 126 APPEVIAYRLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILKV 183
           AP EVIA RLE+RAKEGNHFM S LL+SQLDLL+I+D+EGI+KVDAT TPQ I++NIL +
Sbjct: 121 APAEVIALRLEKRAKEGNHFMPSALLKSQLDLLQIEDTEGIVKVDATQTPQAILSNILNL 180

[Close]

BLAST of Moc06g40130 vs. TAIR 10
Match: AT2G16790.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 198.4 bits (503), Expect = 5.3e-51
Identity = 99/177 (55.93%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 13  IIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKV 72
           +I +MGV G+GKSTIG+ML KA++ +FLDADDFH  SN++KM +GI LS+EDR+PWLEK+
Sbjct: 11  VIAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKI 70

Query: 73  RDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIG---SCVVKFVLLDAPPEVIAY 132
           +++L++ +   ++VVL CS+L+KQYREILR +DP+Y+ G   SC V FVLL+   EVIA 
Sbjct: 71  QESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAA 130

Query: 133 RLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSL 187
           RL++RA E  HFM  TLL+SQ DLL+ D+ E I K+   L+P+VIV  IL++V  SL
Sbjct: 131 RLQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTILEMVANSL 187

[Close]

BLAST of Moc06g40130 vs. TAIR 10
Match: AT2G16790.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 198.4 bits (503), Expect = 5.3e-51
Identity = 99/177 (55.93%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 13  IIVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKV 72
           +I +MGV G+GKSTIG+ML KA++ +FLDADDFH  SN++KM +GI LS+EDR+PWLEK+
Sbjct: 9   VIAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKI 68

Query: 73  RDALKESIACKKSVVLGCSALQKQYREILRSADPNYEIG---SCVVKFVLLDAPPEVIAY 132
           +++L++ +   ++VVL CS+L+KQYREILR +DP+Y+ G   SC V FVLL+   EVIA 
Sbjct: 69  QESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAA 128

Query: 133 RLERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSL 187
           RL++RA E  HFM  TLL+SQ DLL+ D+ E I K+   L+P+VIV  IL++V  SL
Sbjct: 129 RLQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTILEMVANSL 185

The following BLAST results are available for this feature:

BLAST of Moc06g40130 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159526.1	1.7e-99	100.00	uncharacterized protein LOC111025918 isoform X1 [Momordica charantia]	[more]
XP_022159527.1	1.9e-82	100.00	uncharacterized protein LOC111025918 isoform X2 [Momordica charantia] >XP_022159...	[more]
XP_023532636.1	1.5e-71	78.31	gluconokinase [Cucurbita pepo subsp. pepo]	[more]
XP_038874456.1	3.3e-71	79.55	gluconokinase [Benincasa hispida] >XP_038874457.1 gluconokinase [Benincasa hispi...	[more]
XP_022995367.1	4.8e-70	78.49	gluconokinase [Cucurbita maxima]	[more]

BLAST of Moc06g40130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SLE0	7.4e-50	55.93	Gluconokinase OS=Arabidopsis thaliana OX=3702 GN=At2g16790 PE=2 SV=2	[more]
P39208	1.0e-27	44.81	Thermosensitive gluconokinase OS=Escherichia coli (strain K12) OX=83333 GN=idnK ...	[more]
B0BML1	1.5e-26	42.29	Probable gluconokinase OS=Xenopus tropicalis OX=8364 GN=idnk PE=2 SV=1	[more]
Q5FQ97	1.1e-24	38.67	Gluconokinase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GOX1709 PE=1 S...	[more]
Q5T6J7	1.1e-24	38.12	Probable gluconokinase OS=Homo sapiens OX=9606 GN=IDNK PE=1 SV=1	[more]

BLAST of Moc06g40130 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E468	8.1e-100	100.00	Gluconokinase OS=Momordica charantia OX=3673 GN=LOC111025918 PE=3 SV=1	[more]
A0A6J1DZ23	9.0e-83	100.00	Gluconokinase OS=Momordica charantia OX=3673 GN=LOC111025918 PE=3 SV=1	[more]
A0A6J1JYN8	2.3e-70	78.49	Gluconokinase OS=Cucurbita maxima OX=3661 GN=LOC111490938 PE=3 SV=1	[more]
A0A6J1H380	8.7e-70	76.72	Gluconokinase OS=Cucurbita moschata OX=3662 GN=LOC111459704 PE=3 SV=1	[more]
A0A0A0KN86	1.3e-68	75.69	Gluconokinase OS=Cucumis sativus OX=3659 GN=Csa_5G169050 PE=3 SV=1	[more]

BLAST of Moc06g40130 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G16790.1	5.3e-51	55.93	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G16790.2	5.3e-51	55.93	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 7..183 e-value: 5.6E-59 score: 200.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 10..181
IPR031322	Shikimate kinase/gluconokinase	PFAM	PF01202	SKI	coord: 21..143 e-value: 3.2E-14 score: 53.3
IPR006001	Carbohydrate kinase, thermoresistant glucokinase	TIGRFAM	TIGR01313	TIGR01313	coord: 14..182 e-value: 1.9E-47 score: 159.0
IPR006001	Carbohydrate kinase, thermoresistant glucokinase	PANTHER	PTHR43442	GLUCONOKINASE-RELATED	coord: 10..182
IPR006001	Carbohydrate kinase, thermoresistant glucokinase	CDD	cd02021	GntK	coord: 13..160 e-value: 2.2581E-58 score: 178.215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g40130.1	Moc06g40130.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046177	D-gluconate catabolic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046316	gluconokinase activity
molecular_function	GO:0016301	kinase activity