Moc06g38360 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc06g38360 vs. NCBI nr
Match: XP_038906749.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 830.9 bits (2145), Expect = 5.6e-237
Identity = 394/458 (86.03%), Postives = 428/458 (93.45%), Query Frame = 0

Query: 9   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 68
           +AVI+LALVSLATV+G+KPCP +N  E PAI+CRK++AVLT+FG VGDGVTSNTHAFRQA
Sbjct: 18  SAVIILALVSLATVEGKKPCPTTNYLEVPAISCRKHTAVLTDFGGVGDGVTSNTHAFRQA 77

Query: 69  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 128
           IEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HK A ILASQDESEWPLVA+LPS
Sbjct: 78  IEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKYATILASQDESEWPLVAVLPS 137

Query: 129 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 188
           YGRGRDAPGGR+SSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHK+ELNVTRPYLIE+M
Sbjct: 138 YGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKDELNVTRPYLIEMM 197

Query: 189 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 248
           YSDQIQIS+LTLVNSPSWFVHPIYS NVII+GLTILAPIDSPNTDGIDPDSC+NTRIEDC
Sbjct: 198 YSDQIQISDLTLVNSPSWFVHPIYSRNVIIRGLTILAPIDSPNTDGIDPDSCSNTRIEDC 257

Query: 249 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 308
           FIVSGDDCIAVKSGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 309 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 368
           +VT INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDP A+P
Sbjct: 318 NVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPTAVP 377

Query: 369 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 428
           EI NINYRDVVAENVTY+ARLEGISGDPFTDIC++NV IGLT KPKKLQWNCT+VEGFSS
Sbjct: 378 EITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSS 437

Query: 429 GVVPPPCAPLAKPAEGVGCAFPTDRLPIEEFPLKTCSI 467
            V+PPPCAPLAK A+  GC FP D LPIE+  LKTC +
Sbjct: 438 DVMPPPCAPLAKAAKSGGCYFPEDELPIEKVQLKTCFV 475

BLAST of Moc06g38360 vs. NCBI nr
Match: XP_023532553.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 824.7 bits (2129), Expect = 4.0e-235
Identity = 394/457 (86.21%), Postives = 424/457 (92.78%), Query Frame = 0

Query: 9   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 68
           +AVILLA VSL +VDG+KPCP S+  EFPAI+CRK++AVLT+FG VGDGVTSNT+AFR+A
Sbjct: 18  SAVILLAFVSLGSVDGKKPCPFSSYLEFPAISCRKHTAVLTDFGGVGDGVTSNTNAFREA 77

Query: 69  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 128
           +EHL  LA  GGAQL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWPLV +LPS
Sbjct: 78  MEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWPLVEVLPS 137

Query: 129 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 188
           YGRGRDAPGGRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFH+NELNVTRPY+IEIM
Sbjct: 138 YGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHRNELNVTRPYMIEIM 197

Query: 189 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 248
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC NTRIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDC 257

Query: 249 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 308
           FIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 309 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 368
           +VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTG+YKSHPD +FDP A+P
Sbjct: 318 NVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGNYKSHPDEKFDPKALP 377

Query: 369 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 428
           EIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++NV IGLT KPKKLQWNCTNVEG+SS
Sbjct: 378 EIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTAKPKKLQWNCTNVEGYSS 437

Query: 429 GVVPPPCAPLAKP-AEGVGCAFPTDRLPIEEFPLKTC 465
            VVPPPCAPLAK      GC FP DRLPIE+  L TC
Sbjct: 438 DVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDVKLMTC 474

BLAST of Moc06g38360 vs. NCBI nr
Match: XP_022996001.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 817.0 bits (2109), Expect = 8.4e-233
Identity = 392/457 (85.78%), Postives = 422/457 (92.34%), Query Frame = 0

Query: 9   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 68
           +AVILL LVSLA+VDG+KPCP S+  EF AI+CRK++AVLT+FG VGDGVTSNT+AFR+A
Sbjct: 18  SAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDFGGVGDGVTSNTNAFREA 77

Query: 69  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 128
           IEHL  LA  GG+QL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWPLV +LPS
Sbjct: 78  IEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWPLVEVLPS 137

Query: 129 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 188
           YGRGRDAPGGRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHKNE NVTRPY+IEIM
Sbjct: 138 YGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNEFNVTRPYMIEIM 197

Query: 189 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 248
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC NTRIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDC 257

Query: 249 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 308
           FIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 309 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 368
           +VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTG+YKSHPD +FDP A+P
Sbjct: 318 NVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGNYKSHPDEKFDPKALP 377

Query: 369 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 428
           EIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++NV IGLT KPKKLQWNCTNVEG+SS
Sbjct: 378 EIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTAKPKKLQWNCTNVEGYSS 437

Query: 429 GVVPPPCAPLAKP-AEGVGCAFPTDRLPIEEFPLKTC 465
            VVP PCAPLAK      GC FP D+LPIE+  L TC
Sbjct: 438 DVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTC 474

BLAST of Moc06g38360 vs. NCBI nr
Match: XP_008437531.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative polygalacturonase [Cucumis melo var. makuwa] >TYK05998.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 815.5 bits (2105), Expect = 2.5e-232
Identity = 390/458 (85.15%), Postives = 424/458 (92.58%), Query Frame = 0

Query: 9   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 68
           +AVILLALVSLATV+G++ CP +N  + PAINCR+++AVLT+FG VGDGVTSNT AFR+A
Sbjct: 18  SAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDFGGVGDGVTSNTQAFRRA 77

Query: 69  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 128
           IEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA ILASQDESEWP VAILPS
Sbjct: 78  IEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATILASQDESEWPQVAILPS 137

Query: 129 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 188
           YG GRDAPGGR+SSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHK ELN+TRPY+IEI+
Sbjct: 138 YGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKGELNLTRPYMIEIL 197

Query: 189 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 248
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC+N RIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNIRIEDC 257

Query: 249 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 308
           FIVSGDDCIAVKSGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 309 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 368
           +VT INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+P
Sbjct: 318 NVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAALP 377

Query: 369 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 428
           EI NINYRDVVAENVTY+ARLEGISGDPFT+IC++NV IGLT KPKKLQWNCT+VEG SS
Sbjct: 378 EITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGISS 437

Query: 429 GVVPPPCAPLAKPAEGVGCAFPTDRLPIEEFPLKTCSI 467
            VVPPPCAPLAK A+  GC FP D+LPIE+  LKTC +
Sbjct: 438 DVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFV 474

BLAST of Moc06g38360 vs. NCBI nr
Match: XP_022957618.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 815.5 bits (2105), Expect = 2.5e-232
Identity = 392/464 (84.48%), Postives = 424/464 (91.38%), Query Frame = 0

Query: 6   QVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSN 65
           Q++AA    VILLALVSL +VDG+K CP S+  EFPAI+CRK++AVLT+FG +GDGVTSN
Sbjct: 11  QITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDFGGIGDGVTSN 70

Query: 66  THAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWP 125
           T+AFR+A+EHL  LA  GGAQL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWP
Sbjct: 71  TNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWP 130

Query: 126 LVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTR 185
           LV +LPSYGRGRDAP GRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHKNE NVTR
Sbjct: 131 LVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNEFNVTR 190

Query: 186 PYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCT 245
           PY+IEIMYS+QIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC 
Sbjct: 191 PYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCI 250

Query: 246 NTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGG 305
           NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGG
Sbjct: 251 NTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGG 310

Query: 306 IRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPE 365
           IR+VRIE+VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTGDYKSHPD +
Sbjct: 311 IRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGDYKSHPDEK 370

Query: 366 FDPAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCT 425
           FDP A+PEIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++N+ IGLT K KKLQWNCT
Sbjct: 371 FDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNIKIGLTAKQKKLQWNCT 430

Query: 426 NVEGFSSGVVPPPCAPLAKP-AEGVGCAFPTDRLPIEEFPLKTC 465
           NVEG+SS VVPPPCAPLAK      GC FP DRLPIE+  L TC
Sbjct: 431 NVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDLKLMTC 474

BLAST of Moc06g38360 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 7.3e-179
Identity = 300/471 (63.69%), Postives = 365/471 (77.49%), Query Frame = 0

Query: 4   FGQVSAAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTH 63
           F  VS  V ++ L+S++  + RK        E+ AI+CR +SA L +FG VGDG T NT 
Sbjct: 22  FQVVSVLVAVVLLLSVSRGECRKG-RILEALEYSAISCRAHSASLVDFGGVGDGQTLNTK 81

Query: 64  AFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLV 123
           AF+ A+  L    + GGAQL+VPAGKWLTGSF+LTSHFTLFLH+DA +LASQD S+WP++
Sbjct: 82  AFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWPVI 141

Query: 124 AILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPY 183
             LPSYGRGRDA  GR++SLI+GTNLTDV+ITG+NGTIDGQG  WW +FH  +L  TRPY
Sbjct: 142 KPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPY 201

Query: 184 LIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNT 243
           LIE+MYS  IQISNLTL+NSPSW VHP+YS N++IQG+TILAP+ SPNTDGI+PDSCTNT
Sbjct: 202 LIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNT 261

Query: 244 RIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIR 303
           RIEDC+IVSGDDC+AVKSGWD+YGI +GMPT+ L+IRRLTCISP SA IALGSEMSGGI+
Sbjct: 262 RIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQ 321

Query: 304 DVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFD 363
           DVR ED+ AIN++S +RIKT  GRGG+VKDI+VR M + TMK+ FWMTG+Y SH D  +D
Sbjct: 322 DVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYD 381

Query: 364 PAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNV 423
           P A P I+ INYRD+VAENV+ AARLEGI  DPFT IC++NVTI L  K KK+ W CT+V
Sbjct: 382 PKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDV 441

Query: 424 EGFSSGVVPPPCAPLAK--PAEGVGCAFPTDRLPIEEFPLKTCSISTCSFP 473
           EG SSGV P PC+ L    P +   C FP + LPI+   L+ CS     +P
Sbjct: 442 EGISSGVTPTPCSTLPDQGPEKTSLCNFPAESLPIDTVELQKCSYGINYYP 491

BLAST of Moc06g38360 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.3e-30
Identity = 116/403 (28.78%), Postives = 179/403 (44.42%), Query Frame = 0

Query: 48  LTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKW-------LTGSFNLTSH 107
           +T+FGAVGDGVT ++ AF +A E +   +  G  Q  VPAG           GS   T  
Sbjct: 26  VTQFGAVGDGVTDDSQAFLKAWEAV--CSGTGDGQFVVPAGMTFMLQPLKFQGSCKSTPV 85

Query: 108 FTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGT 167
           F   L K                 + PS G  +    G     I  T++  +VI G +G 
Sbjct: 86  FVQMLGK----------------LVAPSKGNWK----GDKDQWILFTDIEGLVIEG-DGE 145

Query: 168 IDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQG 227
           I+GQGS WW+  HK     +RP  ++    + +++S LT ++SP   +H    + V I  
Sbjct: 146 INGQGSSWWE--HKG----SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISS 205

Query: 228 LTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIR 287
           L I AP  SPNTDGID  + +N  I+DC I +GDDCIA+ SG           T ++ I 
Sbjct: 206 LRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNIHIS 265

Query: 288 RLTCISPDSAGIALGSEMSGG----IRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFV 347
            + C      GI++GS    G    + +V +++     T +  RIKT +G  G+ + I  
Sbjct: 266 GIDC--GPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITF 325

Query: 348 RRMFLSTMKY-----VFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEG 407
             + L  ++       F+  GD  +  D +     + ++   N+  +      Y      
Sbjct: 326 NGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNF--IGTSKSEYGVDFRC 384

Query: 408 ISGDPFTDICVTNVTI--GLTKKPKKLQWNCTNVEGFSSGVVP 433
               P T+I + ++ I    +   +  Q  C NV G S+  VP
Sbjct: 386 SERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVP 384

BLAST of Moc06g38360 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-28
Identity = 121/408 (29.66%), Postives = 174/408 (42.65%), Query Frame = 0

Query: 50  EFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGS---FNLTSHFTLFLH 109
           ++GAVGDG   +T AF               A L VPA K    +   F       L   
Sbjct: 63  QYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLSFK 122

Query: 110 KDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGS 169
            D  I+A  D + W    I   + +                 LTD  + G  G IDGQG 
Sbjct: 123 VDGTIVAQPDPARWKNSKIWLQFAQ-----------------LTDFNLMG-TGVIDGQGQ 182

Query: 170 YWWDKFHK--------NELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVII 229
            WW    K        N+ N  RP  I+I YS  + +  LTL+NSP + +       V I
Sbjct: 183 QWWAGQCKVVNGRTVCNDRN--RPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKI 242

Query: 230 QGLTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLL 289
           QGL I AP DSPNTDGID  +     IE C I +GDDCIA+ +G           + ++ 
Sbjct: 243 QGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG-----------SSNIT 302

Query: 290 IRRLTCISPDSAGIALGS----EMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDI 349
           I+ L C      GI++GS         +  V +     I+TQ+ +RIKT +G  G    I
Sbjct: 303 IKDLIC--GPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYI 362

Query: 350 FVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAA-RLEGIS 409
               + +   +    +   Y +      +  +  +I+ + Y+++   + T AA +L    
Sbjct: 363 TYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSD 422

Query: 410 GDPFTDICVTNVTIGLTK-KPKKLQWNCT--NVEGFSSGVVPPPCAPL 439
             P T I ++NV++ LT  KP     +C   N  GF SG + P C  L
Sbjct: 423 SVPCTGIQLSNVSLKLTSGKPA----SCVDKNARGFYSGRLIPTCKNL 430

BLAST of Moc06g38360 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 7.6e-27
Identity = 117/407 (28.75%), Postives = 177/407 (43.49%), Query Frame = 0

Query: 51  FGAVGDGVTSNTHAFRQAIEHLKPLAAAG--GAQLFVPAGKWLTGSFNLT------SHFT 110
           +GAVGDG   +T AF +        AA     A   VPA K    + NL        HF+
Sbjct: 65  YGAVGDGKHDSTDAFEKTWN-----AACNKLSAVFLVPANKKFVVN-NLVFYGPCQPHFS 124

Query: 111 LFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTID 170
             +  D  I A  D ++W    I   + R                 LTD  + G  G ID
Sbjct: 125 FKV--DGTIAAYPDPAKWKNSKIWMHFAR-----------------LTDFNLMG-TGVID 184

Query: 171 GQGSYWWDKFHKNELNVT------RPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNV 230
           GQG+ WW    K     T      RP  I+I +S  + +  LTL NSP + +       V
Sbjct: 185 GQGNRWWSDQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGV 244

Query: 231 IIQGLTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEH 290
            IQG+ I AP DSPNTDGID  +     IE C I +GDDC+AV +G           + +
Sbjct: 245 KIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG-----------SSN 304

Query: 291 LLIRRLTCISPDSAGIALGSEMSGGIRD----VRIEDVTAINTQSAVRIKTARGRGGFVK 350
           + I+ LTC      G+++GS   G  R     V ++    I+TQ+ +RIKT +G  G   
Sbjct: 305 ITIKDLTC--GPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLAS 364

Query: 351 DIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAA-RLEG 410
            I    + +   +    +   Y +      +  +  +I+++ ++++   + T AA +L  
Sbjct: 365 HITYENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMC 424

Query: 411 ISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPL 439
               P ++I ++NV + LT        N  N  G+ +  + P C  L
Sbjct: 425 SDSVPCSNIKLSNVFLKLTSGKVATCVN-KNANGYYTNPLNPSCKSL 431

BLAST of Moc06g38360 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 2.9e-26
Identity = 106/399 (26.57%), Postives = 174/399 (43.61%), Query Frame = 0

Query: 48  LTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGK-WLTGSFNLTS--HFTLF 107
           +++FGA GDG T +T AF  A +  K  ++ G   L VP G  +L  S  LT   +  L 
Sbjct: 70  VSDFGAKGDGKTDDTQAFVNAWK--KACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILT 129

Query: 108 LHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQ 167
           +     + ASQ  S++  ++              ++       NL+  V  G+ G +DG 
Sbjct: 130 VQIFGTLSASQKRSDYKDIS--------------KWIMFDGVNNLS--VDGGDTGVVDGN 189

Query: 168 GSYWWD---KFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGL 227
           G  WW    K +K +     P  +    S  + + NL + N+    +     SNV +  +
Sbjct: 190 GETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNV 249

Query: 228 TILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRR 287
            + AP DSPNTDGI   +  N R+ +  I +GDDCI+++SG           ++++ I  
Sbjct: 250 VVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQNVQIND 309

Query: 288 LTCISPDSAGIALGS----EMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVR 347
           +TC      GI++GS         +  V ++      T + VRIKT +G  G   +I  +
Sbjct: 310 ITC--GPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQ 369

Query: 348 RMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISGD-P 407
            + +  +K    +  DY        + +A+ ++KN+ YRD+   + +  A     S + P
Sbjct: 370 NIQMDNVKNPIIIDQDYCDKSKCTTEKSAV-QVKNVVYRDISGTSASENAITFNCSKNYP 429

Query: 408 FTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPC 436
              I +  V I      K  +  CTN      G V P C
Sbjct: 430 CQGIVLDRVNI------KGGKATCTNANVVDKGAVLPQC 430

BLAST of Moc06g38360 vs. ExPASy TrEMBL
Match: A0A6J1K3G8 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 4.1e-233
Identity = 392/457 (85.78%), Postives = 422/457 (92.34%), Query Frame = 0

Query: 9   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 68
           +AVILL LVSLA+VDG+KPCP S+  EF AI+CRK++AVLT+FG VGDGVTSNT+AFR+A
Sbjct: 18  SAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDFGGVGDGVTSNTNAFREA 77

Query: 69  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 128
           IEHL  LA  GG+QL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWPLV +LPS
Sbjct: 78  IEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWPLVEVLPS 137

Query: 129 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 188
           YGRGRDAPGGRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHKNE NVTRPY+IEIM
Sbjct: 138 YGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNEFNVTRPYMIEIM 197

Query: 189 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 248
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC NTRIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDC 257

Query: 249 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 308
           FIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 309 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 368
           +VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTG+YKSHPD +FDP A+P
Sbjct: 318 NVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGNYKSHPDEKFDPKALP 377

Query: 369 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 428
           EIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++NV IGLT KPKKLQWNCTNVEG+SS
Sbjct: 378 EIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTAKPKKLQWNCTNVEGYSS 437

Query: 429 GVVPPPCAPLAKP-AEGVGCAFPTDRLPIEEFPLKTC 465
            VVP PCAPLAK      GC FP D+LPIE+  L TC
Sbjct: 438 DVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTC 474

BLAST of Moc06g38360 vs. ExPASy TrEMBL
Match: A0A5D3C461 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00950 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.2e-232
Identity = 390/458 (85.15%), Postives = 424/458 (92.58%), Query Frame = 0

Query: 9   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 68
           +AVILLALVSLATV+G++ CP +N  + PAINCR+++AVLT+FG VGDGVTSNT AFR+A
Sbjct: 18  SAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDFGGVGDGVTSNTQAFRRA 77

Query: 69  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 128
           IEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA ILASQDESEWP VAILPS
Sbjct: 78  IEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATILASQDESEWPQVAILPS 137

Query: 129 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 188
           YG GRDAPGGR+SSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHK ELN+TRPY+IEI+
Sbjct: 138 YGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKGELNLTRPYMIEIL 197

Query: 189 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 248
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC+N RIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNIRIEDC 257

Query: 249 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 308
           FIVSGDDCIAVKSGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 309 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 368
           +VT INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+P
Sbjct: 318 NVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAALP 377

Query: 369 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 428
           EI NINYRDVVAENVTY+ARLEGISGDPFT+IC++NV IGLT KPKKLQWNCT+VEG SS
Sbjct: 378 EITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGISS 437

Query: 429 GVVPPPCAPLAKPAEGVGCAFPTDRLPIEEFPLKTCSI 467
            VVPPPCAPLAK A+  GC FP D+LPIE+  LKTC +
Sbjct: 438 DVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFV 474

BLAST of Moc06g38360 vs. ExPASy TrEMBL
Match: A0A6J1H0R5 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.2e-232
Identity = 392/464 (84.48%), Postives = 424/464 (91.38%), Query Frame = 0

Query: 6   QVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSN 65
           Q++AA    VILLALVSL +VDG+K CP S+  EFPAI+CRK++AVLT+FG +GDGVTSN
Sbjct: 11  QITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDFGGIGDGVTSN 70

Query: 66  THAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWP 125
           T+AFR+A+EHL  LA  GGAQL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWP
Sbjct: 71  TNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWP 130

Query: 126 LVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTR 185
           LV +LPSYGRGRDAP GRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHKNE NVTR
Sbjct: 131 LVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNEFNVTR 190

Query: 186 PYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCT 245
           PY+IEIMYS+QIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC 
Sbjct: 191 PYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCI 250

Query: 246 NTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGG 305
           NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGG
Sbjct: 251 NTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGG 310

Query: 306 IRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPE 365
           IR+VRIE+VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTGDYKSHPD +
Sbjct: 311 IRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGDYKSHPDEK 370

Query: 366 FDPAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCT 425
           FDP A+PEIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++N+ IGLT K KKLQWNCT
Sbjct: 371 FDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNIKIGLTAKQKKLQWNCT 430

Query: 426 NVEGFSSGVVPPPCAPLAKP-AEGVGCAFPTDRLPIEEFPLKTC 465
           NVEG+SS VVPPPCAPLAK      GC FP DRLPIE+  L TC
Sbjct: 431 NVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDLKLMTC 474

BLAST of Moc06g38360 vs. ExPASy TrEMBL
Match: A0A1S3AUV5 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.2e-232
Identity = 390/458 (85.15%), Postives = 424/458 (92.58%), Query Frame = 0

Query: 9   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 68
           +AVILLALVSLATV+G++ CP +N  + PAINCR+++AVLT+FG VGDGVTSNT AFR+A
Sbjct: 18  SAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDFGGVGDGVTSNTQAFRRA 77

Query: 69  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 128
           IEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA ILASQDESEWP VAILPS
Sbjct: 78  IEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATILASQDESEWPQVAILPS 137

Query: 129 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 188
           YG GRDAPGGR+SSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHK ELN+TRPY+IEI+
Sbjct: 138 YGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKGELNLTRPYMIEIL 197

Query: 189 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 248
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC+N RIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNIRIEDC 257

Query: 249 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 308
           FIVSGDDCIAVKSGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 309 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 368
           +VT INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+P
Sbjct: 318 NVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAALP 377

Query: 369 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 428
           EI NINYRDVVAENVTY+ARLEGISGDPFT+IC++NV IGLT KPKKLQWNCT+VEG SS
Sbjct: 378 EITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGISS 437

Query: 429 GVVPPPCAPLAKPAEGVGCAFPTDRLPIEEFPLKTCSI 467
            VVPPPCAPLAK A+  GC FP D+LPIE+  LKTC +
Sbjct: 438 DVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFV 474

BLAST of Moc06g38360 vs. ExPASy TrEMBL
Match: A0A0A0KNF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 4.8e-226
Identity = 379/460 (82.39%), Postives = 421/460 (91.52%), Query Frame = 0

Query: 9   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 68
           +AVILLAL+SLATV+G++ CP +N  + PAI+CRK++A++T+FG VGDGVTSNT AFR+A
Sbjct: 18  SAVILLALLSLATVEGKRSCP-TNYLQVPAISCRKHTALITDFGGVGDGVTSNTQAFRRA 77

Query: 69  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQ--DESEWPLVAIL 128
           IEHL  LAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA+IL SQ  +ESEWP VA+L
Sbjct: 78  IEHLSSLAADGGAQLTVPPGKWLTGSFNLTSHFTLFVHKDAKILGSQVENESEWPQVALL 137

Query: 129 PSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIE 188
           PSYG GRDAPGGR+SSLI+GTNLTDVVITGNNGTIDGQG+YWWDKFHK ELN+TRPYLIE
Sbjct: 138 PSYGVGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKGELNLTRPYLIE 197

Query: 189 IMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIE 248
           IMYSDQIQIS+LTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC+NTRIE
Sbjct: 198 IMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIE 257

Query: 249 DCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVR 308
           DCFIVSGDDCIAVKSGWDQYGI+FGMPTE L+I+RLTCISPDSAG+ALGSEMSGGIR+VR
Sbjct: 258 DCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGVALGSEMSGGIRNVR 317

Query: 309 IEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAA 368
           IE+ T INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA
Sbjct: 318 IENFTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAA 377

Query: 369 IPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGF 428
           +PEI NINYRDVVAENVTY+ARLEGISGDPFT+IC++NV IGLT  PKKLQWNCT+VEGF
Sbjct: 378 LPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTATPKKLQWNCTDVEGF 437

Query: 429 SSGVVPPPCAPLAKPAEGVGCAFPTDRLPIEEFPLKTCSI 467
           SS VVPPPCAPLAK  +  GC FP D+LPIE   LKTC +
Sbjct: 438 SSDVVPPPCAPLAKAGKSGGCDFPEDQLPIERVQLKTCFV 476

BLAST of Moc06g38360 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-185
Identity = 302/434 (69.59%), Postives = 358/434 (82.49%), Query Frame = 0

Query: 35  EFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGS 94
           EF A+NCRK+SA+LT+FGAVGDG TSNT AFR AI  L  +A  GGAQL VP GKWLTGS
Sbjct: 34  EFSALNCRKHSAILTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGS 93

Query: 95  FNLTSHFTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPG-GRFSSLIYGTNLTDVV 154
           FNLTSHFTLF+ + A ILASQDESEWP++A LPSYG+GRD  G GRF+SLI GTNLTDVV
Sbjct: 94  FNLTSHFTLFIQRGATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVV 153

Query: 155 ITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYS 214
           ITGNNGTI+GQG YWWDKF K +  +TRPYLIEI++S  IQISN+TL++SPSW +HP+Y 
Sbjct: 154 ITGNNGTINGQGQYWWDKFKKKQFKITRPYLIEILFSKNIQISNITLIDSPSWNIHPVYC 213

Query: 215 SNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMP 274
           ++VI++ +T+LAP+  PNTDGI+PDSCTNT IEDC+IVSGDDCIAVKSGWDQYGI+FGMP
Sbjct: 214 NSVIVKSVTVLAPVTVPNTDGINPDSCTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMP 273

Query: 275 TEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKD 334
           T+ L IRRLTCISP SAG+ALGSEMSGGI+DVRIEDVT  NT+SA+RIKTA GRG +VKD
Sbjct: 274 TQQLSIRRLTCISPKSAGVALGSEMSGGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVKD 333

Query: 335 IFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGIS 394
           I+ RR+ + TMKYVFWM+G+Y SHPD  FDP A+PEI NINYRD+ AENVT +A L+GI 
Sbjct: 334 IYARRITMKTMKYVFWMSGNYGSHPDEGFDPKALPEITNINYRDMTAENVTMSASLDGID 393

Query: 395 GDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK---PAEGVGCAFP 454
            DPFT IC++NVTI L  K KK+QWNCT+V G +S V P PC+ L +    A+ V CAFP
Sbjct: 394 KDPFTGICISNVTIALAAKAKKMQWNCTDVAGVTSRVTPEPCSLLPEKKAQAKNVDCAFP 453

Query: 455 TDRLPIEEFPLKTC 465
           +D +PIE   LK C
Sbjct: 454 SDLIPIESVVLKKC 467

BLAST of Moc06g38360 vs. TAIR 10
Match: AT4G23500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-185
Identity = 304/432 (70.37%), Postives = 355/432 (82.18%), Query Frame = 0

Query: 35  EFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGS 94
           E+ AI+CR YSA L EFGAVGDGVTSNT AFR A+  L   A  GG+ LFVPAG+WLTG+
Sbjct: 57  EYSAISCRAYSASLDEFGAVGDGVTSNTAAFRDAVSQLSRFADYGGSLLFVPAGRWLTGN 116

Query: 95  FNLTSHFTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVI 154
           FNLTSHFTLFLH+DA ILASQ+ES++ ++  LPSYGRGRD  GGRF SL++G+NLTDVVI
Sbjct: 117 FNLTSHFTLFLHRDAVILASQEESDYEVIEPLPSYGRGRDTDGGRFISLLFGSNLTDVVI 176

Query: 155 TGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSS 214
           TG NGTIDGQG  WW KF + EL  TRPYLIEIM+SD IQISNLT +NSPSW +HP+YSS
Sbjct: 177 TGENGTIDGQGEPWWGKFKRGELKYTRPYLIEIMHSDGIQISNLTFLNSPSWHIHPVYSS 236

Query: 215 NVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPT 274
           N+ IQGLTILAP+  PNTDGI+PDSCTNTRIEDC+IVSGDDCIAVKSGWDQYGI +GMPT
Sbjct: 237 NIYIQGLTILAPVTVPNTDGINPDSCTNTRIEDCYIVSGDDCIAVKSGWDQYGINYGMPT 296

Query: 275 EHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDI 334
           + LLIRRLTCISPDSA IALGSEMSGGI DVR ED+ AIN++S +RIKTA GRGG+VKD+
Sbjct: 297 KQLLIRRLTCISPDSAVIALGSEMSGGIEDVRAEDIVAINSESGIRIKTAIGRGGYVKDV 356

Query: 335 FVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISG 394
           +VR M + TMKYVFWMTG Y SHPD  +DP A+P I+NINY+D+VAENVT  A+L GISG
Sbjct: 357 YVRGMTMMTMKYVFWMTGSYGSHPDDHYDPKALPVIQNINYQDMVAENVTMPAQLAGISG 416

Query: 395 DPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPC--APLAKPAEGVGCAFPTD 454
           D FT IC++NVTI L+KKPKK+ WNCT+V G++SGV P PC   P  +P   V C FP D
Sbjct: 417 DQFTGICISNVTITLSKKPKKVLWNCTDVSGYTSGVTPQPCQLLPEKQPGTVVPCNFPED 476

Query: 455 RLPIEEFPLKTC 465
            +PI+E  L+ C
Sbjct: 477 PIPIDEVKLQRC 488

BLAST of Moc06g38360 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 643.3 bits (1658), Expect = 1.6e-184
Identity = 306/469 (65.25%), Postives = 373/469 (79.53%), Query Frame = 0

Query: 7   VSAAVILLALVSLATV-DGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAF 66
           ++  V ++ L +L T+  GR+   +    E+ AI CR +SA +TE+G VGDG T NT AF
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAF 64

Query: 67  RQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAI 126
           + A++HL   ++ GGAQLFVPAGKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P++  
Sbjct: 65  QSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKA 124

Query: 127 LPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLI 186
           LPSYGRGRDA GGRF+SLI+GTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLI
Sbjct: 125 LPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLI 184

Query: 187 EIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRI 246
           E+M+SD IQISNLT ++SPSW +HP+YSSN+I++G+TI+AP+ SPNTDGI+PDSCTNTRI
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRI 244

Query: 247 EDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDV 306
           EDC+I+SGDDCIAVKSGWD+YGI FGMPT+HL+IRRLTCISP SA IALGSEMSGGI DV
Sbjct: 245 EDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDV 304

Query: 307 RIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPA 366
           R ED+TA  T+S VRIKTA GRG FVK+I+V+ M L TMK+VFWMTG+YK+H D  +DP 
Sbjct: 305 RAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPH 364

Query: 367 AIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEG 426
           A+PEI  INYRD+VAENV+ A RLEGISGDPFT IC++N TI +  K KK  W C++VEG
Sbjct: 365 ALPEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEG 424

Query: 427 FSSGVVPPPCAPL---------AKPAEGVGCAFPTDRLPIEEFPLKTCS 466
            +SGV P PC  L          K  +G GC FPTD L I+   LKTCS
Sbjct: 425 VTSGVDPKPCDLLDGQESETTKKKMIDG-GCDFPTDVLEIDNVELKTCS 472

BLAST of Moc06g38360 vs. TAIR 10
Match: AT3G61490.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 643.3 bits (1658), Expect = 1.6e-184
Identity = 306/469 (65.25%), Postives = 373/469 (79.53%), Query Frame = 0

Query: 7   VSAAVILLALVSLATV-DGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAF 66
           ++  V ++ L +L T+  GR+   +    E+ AI CR +SA +TE+G VGDG T NT AF
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAF 64

Query: 67  RQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAI 126
           + A++HL   ++ GGAQLFVPAGKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P++  
Sbjct: 65  QSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKA 124

Query: 127 LPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLI 186
           LPSYGRGRDA GGRF+SLI+GTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLI
Sbjct: 125 LPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLI 184

Query: 187 EIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRI 246
           E+M+SD IQISNLT ++SPSW +HP+YSSN+I++G+TI+AP+ SPNTDGI+PDSCTNTRI
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRI 244

Query: 247 EDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDV 306
           EDC+I+SGDDCIAVKSGWD+YGI FGMPT+HL+IRRLTCISP SA IALGSEMSGGI DV
Sbjct: 245 EDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDV 304

Query: 307 RIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPA 366
           R ED+TA  T+S VRIKTA GRG FVK+I+V+ M L TMK+VFWMTG+YK+H D  +DP 
Sbjct: 305 RAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPH 364

Query: 367 AIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEG 426
           A+PEI  INYRD+VAENV+ A RLEGISGDPFT IC++N TI +  K KK  W C++VEG
Sbjct: 365 ALPEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEG 424

Query: 427 FSSGVVPPPCAPL---------AKPAEGVGCAFPTDRLPIEEFPLKTCS 466
            +SGV P PC  L          K  +G GC FPTD L I+   LKTCS
Sbjct: 425 VTSGVDPKPCDLLDGQESETTKKKMIDG-GCDFPTDVLEIDNVELKTCS 472

BLAST of Moc06g38360 vs. TAIR 10
Match: AT3G61490.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 643.3 bits (1658), Expect = 1.6e-184
Identity = 306/469 (65.25%), Postives = 373/469 (79.53%), Query Frame = 0

Query: 7   VSAAVILLALVSLATV-DGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAF 66
           ++  V ++ L +L T+  GR+   +    E+ AI CR +SA +TE+G VGDG T NT AF
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAF 64

Query: 67  RQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAI 126
           + A++HL   ++ GGAQLFVPAGKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P++  
Sbjct: 65  QSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKA 124

Query: 127 LPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLI 186
           LPSYGRGRDA GGRF+SLI+GTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLI
Sbjct: 125 LPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLI 184

Query: 187 EIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRI 246
           E+M+SD IQISNLT ++SPSW +HP+YSSN+I++G+TI+AP+ SPNTDGI+PDSCTNTRI
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRI 244

Query: 247 EDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDV 306
           EDC+I+SGDDCIAVKSGWD+YGI FGMPT+HL+IRRLTCISP SA IALGSEMSGGI DV
Sbjct: 245 EDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDV 304

Query: 307 RIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPA 366
           R ED+TA  T+S VRIKTA GRG FVK+I+V+ M L TMK+VFWMTG+YK+H D  +DP 
Sbjct: 305 RAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPH 364

Query: 367 AIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEG 426
           A+PEI  INYRD+VAENV+ A RLEGISGDPFT IC++N TI +  K KK  W C++VEG
Sbjct: 365 ALPEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEG 424

Query: 427 FSSGVVPPPCAPL---------AKPAEGVGCAFPTDRLPIEEFPLKTCS 466
            +SGV P PC  L          K  +G GC FPTD L I+   LKTCS
Sbjct: 425 VTSGVDPKPCDLLDGQESETTKKKMIDG-GCDFPTDVLEIDNVELKTCS 472