Moc06g12290 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g12290
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	isoleucine N-monooxygenase 2-like
Location	chr6: 9371208 .. 9373007 (-)
RNA-Seq Expression	Moc06g12290
Synteny	Moc06g12290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAAGATGCGCAAAGTACTTCACAAATACTGAATCCCTCCACCCTCCGTCAAATGCTTGCTCATAGAACCCACGAAGCCGACACTGTTCTACGCTACATTTTCAGCCTCACCAAGTCCGAAGGTGGTGCGGTGGTCAACTTGAGAAGCATAACGCTTCATTATTGTGGCAACGTTGTCAGAAGAATGCTATTCAATAGAAGATACTACGATACCGGCCGGGAAGATAGAGGGCCAACATTTGAAGAAGAAGAGCACCTTCAAGCATTACTAACATTGCTTATGCATGTCAGTGCCTTTTCTGTGTCGGATTTGATACCTTGTTTGATCTCGACAAACGTCAGAAGATTGTAAAAGAAGGCTTGGAAATGGTGAGAAAGCATGATGAACCGATTGTAGATGAGAGAGTGCAACAACGGAAAGATGGGAATAGGAAAGAGATTCAAGATTTTCTTGATATCTTTATCTCGCTTAGAGATGAAAATGGAAAATCGTTGCTATCGATCGAAGAGATTAAAGCTGAAATCACGGTGAGAATTCTTGTCACACATACAAGAATAATATTTATTTAGGTACACTCATTTTTCACCGACTAAACTTTTTTTACATGCAGAAGAGAGAGAGAAAGGGAAAAAAGGATATATTTTGTATTTAAAAAAAACAGTAAAATCGACAAATTTTTATTGGATAAAACTTGCGGTTACTATAGCTAGGTAAGAAAAGTTTACTCCCATTTTTCTCTCTCTTTCCTTCATACATTAAATCTCTCTTATTCATCAACTTTTTAATAGAAAGTACTTTAGGTCTTAAGTAGTGGTGACCGGTCTTAAGTAGTGGTGACCATCATCAAATGCTTGACAACGCAGAAAAAGTAATTCAAAAACAGAACACTTTTTTAAAAAAGTAATGAGCTGTCAAATTTTCAGTGGTGATCAAGATAGAGATAGATTCTTTATCATTGGAACCTAAGTAAGAAGATGTCTACTTATCGATAAACACCTAATTAAGTTTTATATGTCTAATTTGTTGGTGGTCTACTACAAGCTAGCACTAAAGTATTTTTCGAATACAATTATCAGTAAAATTTCATGTGATAGATAAGTTGTTAGCTCTATATATGCATGCATATGTCAACTTAACTTCAACCTTCTCATCGTTCATAATAATAGTGTTAAGACAGCTCATTAAAAGTGTTTCCGGATGGTTGACACATGACATTAGTTTGGTGACTCAGTTAATTTTAGTTTAACAATAGTTTTGTAATTATTGGTTTTTGGAGAATCATTACAGAGAAATTGTTCGTAGGAGTTTTTTAGCTTTCTTGAGAAATATATAAGCCTCTCTGTATTGATCATTTCATTTTTAATGAGAAAGAGGTAGAAGAAAGCTTTTCTCCTGTAATCCATGTTCATTTTATAATTTGTCTAATAGGAGCTACAACGGCAACAATTGACAATCCTTCAAATGTTGTTGAATGGGCAATGGTAAAGTTACTCAATCAACCAAAAATTCTCCAAAAAGTAATCGAAGAACTAGACAGGATTGTTGGAAGAGATAGACTTGTTTAAGAATCTATATCCCACAACTTAAGTATCTTATTGTTTGTGTTAGAGAATCTTTTCGACTCCATTTTCACCGTTCAATGTCCCTCATGTCTCAAATTCTGATACCATTGTAGCTGGCTACTTTATCCCAAAAGGTAGCCAAGTCCTATTAAGTCGAATTGGTCTCGGCCGAAATCCGAAAAGTTTGGGAGGATCCGATGCGATTCAATCCAGAACGCCATCTCAAGAATAA

mRNA sequence

ATGGAAGAAGATGCGCAAAGTACTTCACAAATACTGAATCCCTCCACCCTCCGTCAAATGCTTGCTCATAGAACCCACGAAGCCGACACTGTTCTACGCTACATTTTCAGCCTCACCAAGTCCGAAGGTGGTGCGGTGGTCAACTTGAGAAGCATAACGCTTCATTATTGTGGCAACGTTTGCCTTTTCTGTGTCGGATTTGATACCTTGTTTGATCTCGACAAACGTCAGAAGATTGTAAAAGAAGGCTTGGAAATGGTGAGAAAGCATGATGAACCGATTGTAGATGAGAGAGTGCAACAACGGAAAGATGGGAATAGGAAAGAGATTCAAGATTTTCTTGATATCTTTATCTCGCTTAGAGATGAAAATGGAAAATCGTTGCTATCGATCGAAGAGATTAAAGCTGAAATCACGAGAATCTTTTCGACTCCATTTTCACCGTTCAATGTCCCTCATGTCTCAAATTCTGATACCATTGTAGCTGGCTACTTTATCCCAAAAGGTAGCCAAGTCCTATTAAGTCGAATTGGTCTCGGCCGAAATCCGAAAAGTTTGGGAGGATCCGATGCGATTCAATCCAGAACGCCATCTCAAGAATAA

Coding sequence (CDS)

ATGGAAGAAGATGCGCAAAGTACTTCACAAATACTGAATCCCTCCACCCTCCGTCAAATGCTTGCTCATAGAACCCACGAAGCCGACACTGTTCTACGCTACATTTTCAGCCTCACCAAGTCCGAAGGTGGTGCGGTGGTCAACTTGAGAAGCATAACGCTTCATTATTGTGGCAACGTTTGCCTTTTCTGTGTCGGATTTGATACCTTGTTTGATCTCGACAAACGTCAGAAGATTGTAAAAGAAGGCTTGGAAATGGTGAGAAAGCATGATGAACCGATTGTAGATGAGAGAGTGCAACAACGGAAAGATGGGAATAGGAAAGAGATTCAAGATTTTCTTGATATCTTTATCTCGCTTAGAGATGAAAATGGAAAATCGTTGCTATCGATCGAAGAGATTAAAGCTGAAATCACGAGAATCTTTTCGACTCCATTTTCACCGTTCAATGTCCCTCATGTCTCAAATTCTGATACCATTGTAGCTGGCTACTTTATCCCAAAAGGTAGCCAAGTCCTATTAAGTCGAATTGGTCTCGGCCGAAATCCGAAAAGTTTGGGAGGATCCGATGCGATTCAATCCAGAACGCCATCTCAAGAATAA

Protein sequence

MEEDAQSTSQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNVCLFCVGFDTLFDLDKRQKIVKEGLEMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRIFSTPFSPFNVPHVSNSDTIVAGYFIPKGSQVLLSRIGLGRNPKSLGGSDAIQSRTPSQE

Homology

[Close]

BLAST of Moc06g12290 vs. NCBI nr
Match: XP_022154369.1 (phenylalanine N-monooxygenase-like [Momordica charantia])

HSP 1 Score: 186.4 bits (472), Expect = 2.4e-43
Identity = 108/176 (61.36%), Postives = 119/176 (67.61%), Query Frame = 0


Query: 8   TSQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNVCLFCV-- 67
           TSQILNPSTL QMLAHRTHEADT+LRYIFSLTKS+G AVVNLRS+TLHYCGNV    +  
Sbjct: 160 TSQILNPSTLHQMLAHRTHEADTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFN 219

Query: 68  ----------GFDTL------------------------------FDLDKRQKIVKEGLE 127
                     G  T                               FD+DK QKIVKEGL+
Sbjct: 220 RRYYGAGQEDGGPTFEEEEHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLK 279

Query: 128 MVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRI 142
           +VRKHDEPIVDERVQQ K G RKEI+DFLDIFI+L+ ENGKSLLSIEEIKAEIT +
Sbjct: 280 VVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITEL 335

[Close]

BLAST of Moc06g12290 vs. NCBI nr
Match: XP_022154340.1 (isoleucine N-monooxygenase 2-like [Momordica charantia])

HSP 1 Score: 181.0 bits (458), Expect = 9.9e-42
Identity = 120/282 (42.55%), Postives = 149/282 (52.84%), Query Frame = 0


Query: 8   TSQILNPST-LRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNV---CLF 67
           TS+IL+PST L Q+L HRT+EA  +LRYIF LT    GAVVNLRSIT HYCGNV    LF
Sbjct: 121 TSEILSPSTDLHQILGHRTNEAHILLRYIFKLTAGGAGAVVNLRSITQHYCGNVIRRSLF 180

Query: 68  CVGF----------------------------------DTL-----FDLDKRQKIVKEGL 127
              +                                  D +     FDLD   KI+K+ L
Sbjct: 181 NTRYYGKGSEDGGPSFEEEEHNQALLTILKHINAFSISDFMPCLKPFDLDGHGKIMKQAL 240

Query: 128 EMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRI--- 185
           E++RK+DEPI++ERVQQ KDG R+ ++D LDI ISL+DE+G+SLLSI+EIKA+IT +   
Sbjct: 241 EVLRKYDEPIINERVQQWKDGKREGVEDILDILISLKDESGESLLSIDEIKAQITELQIA 300

[Close]

BLAST of Moc06g12290 vs. NCBI nr
Match: XP_023516703.1 (isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 177.9 bits (450), Expect = 8.4e-41
Identity = 116/282 (41.13%), Postives = 143/282 (50.71%), Query Frame = 0


Query: 8   TSQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNV------- 67
           TS+I NPSTL +ML HR  EADT+LRY+FS T +  G VVN+RSIT HYCGN+       
Sbjct: 140 TSEIFNPSTLHRMLGHRNTEADTLLRYVFSQTSNRDGGVVNVRSITQHYCGNIMRRMIFN 199

Query: 68  ----------------------CLFC-------------VGFDTLFDLDKRQKIVKEGLE 127
                                  LF              V +   FDLD  QK++K+ L 
Sbjct: 200 KRYYGKGREDGGPTFEEEEHDQALFTMIEYIYAFSISDFVTWLKPFDLDGHQKVMKKALN 259

Query: 128 MVRKHDEPIVDERVQQRKDGNRKEIQ-DFLDIFISLRDENGKSLLSIEEIKAEITRI--- 185
           +V+K+DEPI+DERVQ+ KDG  + ++ D LDI ISL+D+N K LLS+ EIKA+IT +   
Sbjct: 260 IVQKYDEPIIDERVQRWKDGKMEGVKDDILDILISLKDKNAKPLLSVAEIKAQITELQIA 319

[Close]

BLAST of Moc06g12290 vs. NCBI nr
Match: XP_022148952.1 (isoleucine N-monooxygenase 2-like [Momordica charantia])

HSP 1 Score: 177.2 bits (448), Expect = 1.4e-40
Identity = 117/285 (41.05%), Postives = 146/285 (51.23%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFSL----TKSEGGAVVNLRSITLHYCGNVC--- 68
           S+I  PSTL QML HRT+EADT+LRYIFSL    T +EGGAVVN+ S+T HYCGN+    
Sbjct: 157 SEIFCPSTLHQMLGHRTNEADTLLRYIFSLTTTNTNNEGGAVVNVGSVTQHYCGNIIRRM 216

Query: 69  -----------------------------------LFCVGFDTL-----FDLDKRQKIVK 128
                                               F +  D +     FDLD  +KI+K
Sbjct: 217 LFNRRYYGKGREDGGPSFEEEEHNQALLTILRHVNAFSIS-DFMPCLKPFDLDGHEKIMK 276

Query: 129 EGLEMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRI 185
             L+ + K+DEPI++ERVQQ KDG R+  +D LDI IS++D NGKSLL+IEEIKA+IT +
Sbjct: 277 RALKALWKYDEPIINERVQQWKDGKREGAEDILDILISIKDGNGKSLLTIEEIKAQITEL 336

[Close]

BLAST of Moc06g12290 vs. NCBI nr
Match: XP_038880438.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 176.8 bits (447), Expect = 1.9e-40
Identity = 118/282 (41.84%), Postives = 143/282 (50.71%), Query Frame = 0


Query: 8   TSQILNPSTLRQMLAHRTHEADTVLRYIFSLT-KSEGGAVVNLRSITLHYCGNVCLFCV- 67
           TS+ILNPSTL +ML  RT EADT+LRYIF+ T  + GGAV+NLRSIT HYCGN+    + 
Sbjct: 161 TSKILNPSTLHRMLGQRTAEADTLLRYIFTQTNNNRGGAVINLRSITQHYCGNILRQMLF 220

Query: 68  -----------GFDTL------------------------------FDLDKRQKIVKEGL 127
                      G  T                               FDLD  QKI+K  L
Sbjct: 221 NRRYYGKGREDGGPTFEEEEHVQALLTIVKHIHAFSISDFMPCLKSFDLDGHQKIMKNAL 280

Query: 128 EMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRI--- 185
            +++K+DEPI+DERVQ+ KD   KE++D LDI ISL+DEN  SLLSIEEIKA+IT +   
Sbjct: 281 NILQKYDEPIIDERVQEWKDKKIKEVEDILDILISLKDENTNSLLSIEEIKAQITDLQIA 340

[Close]

BLAST of Moc06g12290 vs. ExPASy Swiss-Prot
Match: A0A068Q7V0 (Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.3e-25
Identity = 85/283 (30.04%), Postives = 121/283 (42.76%), Query Frame = 0


Query: 8   TSQILNPSTLRQMLAHRTHEADTVLRYIFS-LTKSEGGAVVNLRSITLHYCGNVCLFCV- 67
           TS++L+P   + +   R  EAD ++RY+F+     EG  +V+LR  T HYC NV    + 
Sbjct: 150 TSELLSPMRHKWLTDKRIEEADHLVRYVFNQCNNDEGSGIVDLRLATQHYCANVIKRMIF 209

Query: 68  ------------------------GFDTL-----------------FDLDKRQKIVKEGL 127
                                    FD L                  DLD  +KI+K+ +
Sbjct: 210 NQRYFTEEMKDGGPSVEEQNYVNAVFDMLRYIYAFSASDYISCLRGLDLDGHEKIIKDCI 269

Query: 128 EMVRKHDEPIVDERVQQ-RKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRIFS 185
           ++ RK  +P+++ER+++ +K G  K   D LDI ISL+D +G+ LLS +EIK ++  +  
Sbjct: 270 KLTRKRQDPVIEERIREHQKLGGNKVPVDLLDILISLKDASGQPLLSTDEIKGQVNEMIM 329

[Close]

BLAST of Moc06g12290 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 116.3 bits (290), Expect = 3.9e-25
Identity = 90/284 (31.69%), Postives = 125/284 (44.01%), Query Frame = 0


Query: 8   TSQILNPSTLRQMLAHRTHEADTVLRYIFSL-TKSEGGAV-VNLRSITLHYCGNVC---- 67
           TS+I+ PS    +   RT EAD + RY+++L TK+  G V V++R +  HYCGNV     
Sbjct: 171 TSEIICPSRHAWLHDKRTDEADNLTRYVYNLATKAATGDVAVDVRHVARHYCGNVIRRLM 230

Query: 68  ----------------------------------LFCVGFDTL-----FDLDKRQKIVKE 127
                                              FCV  D L      DLD  +KIVKE
Sbjct: 231 FNRRYFGEPQADGGPGPMEVLHMDAVFTSLGLLYAFCVS-DYLPWLRGLDLDGHEKIVKE 290

Query: 128 GLEMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKA------ 185
               V +  + ++D+R +Q K G R+E++DFLD+ I+L+D  G  LL+IEE+KA      
Sbjct: 291 ANVAVNRLHDTVIDDRWRQWKSGERQEMEDFLDVLITLKDAQGNPLLTIEEVKAQSQDIT 350

[Close]

BLAST of Moc06g12290 vs. ExPASy Swiss-Prot
Match: O81346 (Tryptophan N-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP79B2 PE=1 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 7.4e-24
Identity = 82/281 (29.18%), Postives = 122/281 (43.42%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNVC------- 68
           ++++ P+  R +   R+ E D +  +++++ K+ G   V+ R +T HYCGN         
Sbjct: 160 TELVCPARHRWLHQKRSEENDHLTAWVYNMVKNSGS--VDFRFMTRHYCGNAIKKLMFGT 219

Query: 69  --------------------------------LFCVG----FDTLFDLDKRQKIVKEGLE 128
                                            FC+       T  DL+  +KI++E   
Sbjct: 220 RTFSKNTAPDGGPTVEDVEHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSA 279

Query: 129 MVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIK---------- 185
           ++ K+ +PI+DER++  ++G R +I+DFLDIFIS++DE G  LL+ +EIK          
Sbjct: 280 IMDKYHDPIIDERIKMWREGKRTQIEDFLDIFISIKDEQGNPLLTADEIKPTIKELVMAA 339

[Close]

BLAST of Moc06g12290 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.3e-23
Identity = 84/283 (29.68%), Postives = 120/283 (42.40%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFSLT-KSEGGA--VVNLRSITLHYCGNVC---- 68
           S + +  + + ML  RT EAD ++RYI + + K+ G A  V++LR     Y GNV     
Sbjct: 145 SHVTSKKSFQMMLQKRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMM 204

Query: 69  --------------------------LFC-------------VGFDTLFDLDKRQKIVKE 128
                                     LF              V +    DL+  +K+V  
Sbjct: 205 FGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSN 264

Query: 129 GLEMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRIF 184
            +  V K+++P VDER+ Q ++G  KE QDFLD+FI  +D +GK  LS EEIKA++T + 
Sbjct: 265 AMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTELM 324

[Close]

BLAST of Moc06g12290 vs. ExPASy Swiss-Prot
Match: Q501D8 (Tryptophan N-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP79B3 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.3e-23
Identity = 83/281 (29.54%), Postives = 123/281 (43.77%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNVC------- 68
           ++I+ P+  R +  +R  E D +  +++++ K+     V+LR +T HYCGN         
Sbjct: 162 TEIVCPARHRWLHDNRAEETDHLTAWLYNMVKN--SEPVDLRFVTRHYCGNAIKRLMFGT 221

Query: 69  --------------------------------LFCVG----FDTLFDLDKRQKIVKEGLE 128
                                            FC+       T  DL+  +KI++E   
Sbjct: 222 RTFSEKTEADGGPTLEDIEHMDAMFEGLGFTFAFCISDYLPMLTGLDLNGHEKIMRESSA 281

Query: 129 MVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIK---------- 185
           ++ K+ +PI+DER++  ++G R +I+DFLDIFIS++DE G+ LL+ +EIK          
Sbjct: 282 IMDKYHDPIIDERIKMWREGKRTQIEDFLDIFISIKDEAGQPLLTADEIKPTIKELVMAA 341

[Close]

BLAST of Moc06g12290 vs. ExPASy TrEMBL
Match: A0A6J1DLV4 (phenylalanine N-monooxygenase-like OS=Momordica charantia OX=3673 GN=LOC111021650 PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.1e-43
Identity = 108/176 (61.36%), Postives = 119/176 (67.61%), Query Frame = 0


Query: 8   TSQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNVCLFCV-- 67
           TSQILNPSTL QMLAHRTHEADT+LRYIFSLTKS+G AVVNLRS+TLHYCGNV    +  
Sbjct: 160 TSQILNPSTLHQMLAHRTHEADTLLRYIFSLTKSKGAAVVNLRSVTLHYCGNVIRRVLFN 219

Query: 68  ----------GFDTL------------------------------FDLDKRQKIVKEGLE 127
                     G  T                               FD+DK QKIVKEGL+
Sbjct: 220 RRYYGAGQEDGGPTFEEEEHIQALLALLKYINAFSVSDFIPCLRPFDVDKHQKIVKEGLK 279

Query: 128 MVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRI 142
           +VRKHDEPIVDERVQQ K G RKEI+DFLDIFI+L+ ENGKSLLSIEEIKAEIT +
Sbjct: 280 VVRKHDEPIVDERVQQWKYGKRKEIEDFLDIFIALKYENGKSLLSIEEIKAEITEL 335

[Close]

BLAST of Moc06g12290 vs. ExPASy TrEMBL
Match: A0A6J1DJC5 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021624 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 4.8e-42
Identity = 120/282 (42.55%), Postives = 149/282 (52.84%), Query Frame = 0


Query: 8   TSQILNPST-LRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNV---CLF 67
           TS+IL+PST L Q+L HRT+EA  +LRYIF LT    GAVVNLRSIT HYCGNV    LF
Sbjct: 121 TSEILSPSTDLHQILGHRTNEAHILLRYIFKLTAGGAGAVVNLRSITQHYCGNVIRRSLF 180

Query: 68  CVGF----------------------------------DTL-----FDLDKRQKIVKEGL 127
              +                                  D +     FDLD   KI+K+ L
Sbjct: 181 NTRYYGKGSEDGGPSFEEEEHNQALLTILKHINAFSISDFMPCLKPFDLDGHGKIMKQAL 240

Query: 128 EMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRI--- 185
           E++RK+DEPI++ERVQQ KDG R+ ++D LDI ISL+DE+G+SLLSI+EIKA+IT +   
Sbjct: 241 EVLRKYDEPIINERVQQWKDGKREGVEDILDILISLKDESGESLLSIDEIKAQITELQIA 300

[Close]

BLAST of Moc06g12290 vs. ExPASy TrEMBL
Match: A0A6J1D6G3 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017495 PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 6.9e-41
Identity = 117/285 (41.05%), Postives = 146/285 (51.23%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFSL----TKSEGGAVVNLRSITLHYCGNVC--- 68
           S+I  PSTL QML HRT+EADT+LRYIFSL    T +EGGAVVN+ S+T HYCGN+    
Sbjct: 157 SEIFCPSTLHQMLGHRTNEADTLLRYIFSLTTTNTNNEGGAVVNVGSVTQHYCGNIIRRM 216

Query: 69  -----------------------------------LFCVGFDTL-----FDLDKRQKIVK 128
                                               F +  D +     FDLD  +KI+K
Sbjct: 217 LFNRRYYGKGREDGGPSFEEEEHNQALLTILRHVNAFSIS-DFMPCLKPFDLDGHEKIMK 276

Query: 129 EGLEMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRI 185
             L+ + K+DEPI++ERVQQ KDG R+  +D LDI IS++D NGKSLL+IEEIKA+IT +
Sbjct: 277 RALKALWKYDEPIINERVQQWKDGKREGAEDILDILISIKDGNGKSLLTIEEIKAQITEL 336

[Close]

BLAST of Moc06g12290 vs. ExPASy TrEMBL
Match: A0A6J1H8D3 (LOW QUALITY PROTEIN: isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461430 PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.5e-40
Identity = 117/280 (41.79%), Postives = 144/280 (51.43%), Query Frame = 0


Query: 10  QILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNV---CLFCVG 69
           +ILNPSTL +ML HR  EAD +LRYIFS T + GGAVVN+RSIT HYCGN+    LF   
Sbjct: 126 EILNPSTLHRMLGHRDTEADNLLRYIFSQTSNRGGAVVNVRSITQHYCGNIIRRMLFNQR 185

Query: 70  F----------------------------------DTL-----FDLDKRQKIVKEGLEMV 129
           +                                  D L     FDLD  QK++K  L +V
Sbjct: 186 YYGKGREDGGPTFEEEEHNQALLTILRHVNAFSISDFLPWLKPFDLDGHQKVMKNALNVV 245

Query: 130 RKHDEPIVDERVQQRKDGNRKEIQ-DFLDIFISLRDENGKSLLSIEEIKAEITRI----- 185
           +K+DEPI+D+RVQ+ +D   +E++ D LDI ISL+DEN K LLS+ EIKA+IT +     
Sbjct: 246 QKYDEPIIDKRVQKWEDEKLEEVKDDILDILISLKDENAKPLLSVAEIKAQITELQIATI 305

[Close]

BLAST of Moc06g12290 vs. ExPASy TrEMBL
Match: A0A6J1H9V7 (isoleucine N-monooxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461429 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 2.0e-40
Identity = 118/281 (41.99%), Postives = 142/281 (50.53%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNV-------- 68
           S+ILN STL +ML HR  EADT+LRYIFS T +  GAVVNLRSIT HYCGN+        
Sbjct: 141 SEILNSSTLHRMLGHRNAEADTLLRYIFSQTSNRDGAVVNLRSITQHYCGNIIRRMLFNK 200

Query: 69  ---------------------CLFCV-----GFDTL--------FDLDKRQKIVKEGLEM 128
                                 L  +      F T         FDLD  QKI+K  L +
Sbjct: 201 RYYGKGREDGGPTFEEEEHNQALLTILKHVNAFSTSDFMPWLKPFDLDGHQKIMKNALNV 260

Query: 129 VRKHDEPIVDERVQQRKDGNRKEI-QDFLDIFISLRDENGKSLLSIEEIKAEITRI---- 185
           V+K+DEPI+DERVQ+ KDG  + + +D L+I ISL+DEN K LLS+ EIKA+IT +    
Sbjct: 261 VQKYDEPIIDERVQRWKDGKMEGVKEDILNILISLKDENAKPLLSVAEIKAQITELQIAT 320

[Close]

BLAST of Moc06g12290 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 117.1 bits (292), Expect = 1.6e-26
Identity = 83/270 (30.74%), Postives = 120/270 (44.44%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFS-LTKSEGG----AVVNLRSITLHYCGNVC-- 68
           S + +  + + ML  RT EAD ++RYI +   K+ G     AV++LR +   Y GNV   
Sbjct: 147 SHVTSKKSFKLMLEKRTEEADNLVRYINNRCVKNRGNGNGLAVIDLRFVVRQYSGNVARK 206

Query: 69  ----------------------------LFC-------------VGFDTLFDLDKRQKIV 128
                                       LF              V +    DL+  +K+V
Sbjct: 207 MMFGIRHFGKGSEDESGPGSEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVV 266

Query: 129 KEGLEMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITR 184
            + +  V K+++P VDER+ Q ++G  KE QDFLD+FI  +D NGK  LS EEIKA++T 
Sbjct: 267 ADAMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLDMFIMAKDTNGKPTLSEEEIKAQVTE 326

[Close]

BLAST of Moc06g12290 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 112.1 bits (279), Expect = 5.3e-25
Identity = 82/281 (29.18%), Postives = 122/281 (43.42%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNVC------- 68
           ++++ P+  R +   R+ E D +  +++++ K+ G   V+ R +T HYCGN         
Sbjct: 160 TELVCPARHRWLHQKRSEENDHLTAWVYNMVKNSGS--VDFRFMTRHYCGNAIKKLMFGT 219

Query: 69  --------------------------------LFCVG----FDTLFDLDKRQKIVKEGLE 128
                                            FC+       T  DL+  +KI++E   
Sbjct: 220 RTFSKNTAPDGGPTVEDVEHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSA 279

Query: 129 MVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIK---------- 185
           ++ K+ +PI+DER++  ++G R +I+DFLDIFIS++DE G  LL+ +EIK          
Sbjct: 280 IMDKYHDPIIDERIKMWREGKRTQIEDFLDIFISIKDEQGNPLLTADEIKPTIKELVMAA 339

[Close]

BLAST of Moc06g12290 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 111.3 bits (277), Expect = 9.0e-25
Identity = 83/281 (29.54%), Postives = 123/281 (43.77%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAVVNLRSITLHYCGNVC------- 68
           ++I+ P+  R +  +R  E D +  +++++ K+     V+LR +T HYCGN         
Sbjct: 162 TEIVCPARHRWLHDNRAEETDHLTAWLYNMVKN--SEPVDLRFVTRHYCGNAIKRLMFGT 221

Query: 69  --------------------------------LFCVG----FDTLFDLDKRQKIVKEGLE 128
                                            FC+       T  DL+  +KI++E   
Sbjct: 222 RTFSEKTEADGGPTLEDIEHMDAMFEGLGFTFAFCISDYLPMLTGLDLNGHEKIMRESSA 281

Query: 129 MVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIK---------- 185
           ++ K+ +PI+DER++  ++G R +I+DFLDIFIS++DE G+ LL+ +EIK          
Sbjct: 282 IMDKYHDPIIDERIKMWREGKRTQIEDFLDIFISIKDEAGQPLLTADEIKPTIKELVMAA 341

[Close]

BLAST of Moc06g12290 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 111.3 bits (277), Expect = 9.0e-25
Identity = 84/283 (29.68%), Postives = 120/283 (42.40%), Query Frame = 0


Query: 9   SQILNPSTLRQMLAHRTHEADTVLRYIFSLT-KSEGGA--VVNLRSITLHYCGNVC---- 68
           S + +  + + ML  RT EAD ++RYI + + K+ G A  V++LR     Y GNV     
Sbjct: 139 SHVTSKKSFQMMLQKRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMM 198

Query: 69  --------------------------LFC-------------VGFDTLFDLDKRQKIVKE 128
                                     LF              V +    DL+  +K+V  
Sbjct: 199 FGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSN 258

Query: 129 GLEMVRKHDEPIVDERVQQRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAEITRIF 184
            +  V K+++P VDER+ Q ++G  KE QDFLD+FI  +D +GK  LS EEIKA++T + 
Sbjct: 259 AMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTELM 318

[Close]

BLAST of Moc06g12290 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 85.5 bits (210), Expect = 5.3e-17
Identity = 72/285 (25.26%), Postives = 122/285 (42.81%), Query Frame = 0


Query: 8   TSQILNPSTLRQMLAHRTHEADTVLRYIFSLTKSEGGAV---VNLRSITLHYCGNV---- 67
           T+++++P+TL + L +R  EAD ++ Y+++L +   G+V   +N+R   L YC  V    
Sbjct: 140 TTKLMSPTTLSKTLGYRNIEADNIVTYVYNLCQL--GSVRKPINVRDTILTYCHAVMMRM 199

Query: 68  --------------------------------CLFC------VGFDTLFDLDKRQKIVKE 127
                                           C F       + F   +++DK +  V+E
Sbjct: 200 MFGQRHFDEVVENGGLGPKEKEHMDAIYLALDCFFSFNLTNYIPFLRGWNVDKAETEVRE 259

Query: 128 GLEMVRKHDEPIVDERVQ-QRKDGNRKEIQDFLDIFISLRDENGKSLLSIEEIKAE---- 185
            + ++   ++PI+ ER+   RK G ++  +D+LDI I+L+D+ G  L + +EI+A+    
Sbjct: 260 AVHIINICNDPIIQERIHLWRKKGGKQMEEDWLDILITLKDDQGMHLFTFDEIRAQCKEI 319

The following BLAST results are available for this feature:

BLAST of Moc06g12290 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154369.1	2.4e-43	61.36	phenylalanine N-monooxygenase-like [Momordica charantia]	[more]
XP_022154340.1	9.9e-42	42.55	isoleucine N-monooxygenase 2-like [Momordica charantia]	[more]
XP_023516703.1	8.4e-41	41.13	isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022148952.1	1.4e-40	41.05	isoleucine N-monooxygenase 2-like [Momordica charantia]	[more]
XP_038880438.1	1.9e-40	41.84	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]

BLAST of Moc06g12290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q7V0	2.3e-25	30.04	Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1...	[more]
Q43135	3.9e-25	31.69	Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3	[more]
O81346	7.4e-24	29.18	Tryptophan N-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP79B2 PE=1 SV=...	[more]
Q9FLC8	1.3e-23	29.68	Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV...	[more]
Q501D8	1.3e-23	29.54	Tryptophan N-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP79B3 PE=1 SV=...	[more]

BLAST of Moc06g12290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLV4	1.1e-43	61.36	phenylalanine N-monooxygenase-like OS=Momordica charantia OX=3673 GN=LOC11102165...	[more]
A0A6J1DJC5	4.8e-42	42.55	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021624...	[more]
A0A6J1D6G3	6.9e-41	41.05	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017495...	[more]
A0A6J1H8D3	1.5e-40	41.79	LOW QUALITY PROTEIN: isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=...	[more]
A0A6J1H9V7	2.0e-40	41.99	isoleucine N-monooxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461429 ...	[more]

BLAST of Moc06g12290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35917.1	1.6e-26	30.74	cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene	[more]
AT4G39950.1	5.3e-25	29.18	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT2G22330.1	9.0e-25	29.54	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT5G05260.1	9.0e-25	29.68	cytochrome p450 79a2	[more]
AT1G58260.1	5.3e-17	25.26	cytochrome p450 79c2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 5..143 e-value: 1.6E-7 score: 32.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 144..186 e-value: 1.3E-6 score: 29.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 9..184
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 145..184 e-value: 7.8E-6 score: 24.9
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 144..184
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 70..141 coord: 8..61
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 8..61
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 70..141
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 144..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g12290.1	Moc06g12290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen