Moc06g05250 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc06g05250
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Diacylglycerol kinase
Location	chr6: 3814381 .. 3817228 (-)
RNA-Seq Expression	Moc06g05250
Synteny	Moc06g05250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCACAGCCGACGACGTTACTATTGCTGCTCGCTCCTCCTTAATCGACTCCTTGAGGGGCTGTGGCTTGTCCGGGATCCCAATCGACAAGGCCCACCTCAAGGCCAACCTCATCATGCCGCAGTATCTCCGCTTGGCCCTGCGGGATTCAATTCGCTTCAAAGATCCCACCGCCAAAATCACTACGCCGGAGAACGTCCTGCCGCCGGAGGCTCCTCTGGTCGTCTTCATTAATCCGCGTAGCGGTGGACGCCATGGCCAGAAGCTCAAGCAGCGCCTCCAGCACTTAATCAGCGACGAACAGGTCTATTGTTTTGTTTGTTTCACTACTTGCTATGAAATTGCGCATTCCGATACCCCTTTTGTCTAAAAATCAAAATTTGCCCCAAACTTGGAAAAGTAGACTTTTTGACCTTTTTATTCACGTGTGGCCGTACGTGTCGGGGTTGGAACTTGGATCTTCCTCTGTTTTTCGTGGATATTTTTTTGAGTTCAACGATTGTGGAGTGAGCCCTAAATTTAAGGGAGGTTATTGATTGACGTCTTCTTACCGTCTTCTTGTTAGTTCACTGAGTCAGCATCGCAACTCCAATGCGCGTGTCGCGTCTTGTTTGTTGTTTAAATATTTCAACCAATTAAAAAAAATATAAATAAATAAGATTCTTCAGGCTACGCTTACGACGGTATTATTTTTAATTTTGTATTGAGAAAGTTGGAATTTGGTGTATTGGGCAATAAAATTTGACGCTTTTGCTACAGAGAGTGGATTAGATTGACGTTAAAGTTTTGTGTTTGTTGGTTGCAGGTTTTCGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTACGGACTGCGTTGCCTGGAAATCTTGTCTGATCTTGGCGACGCTTGTGCCAAACAAACTCGTCAAAAGTTAAGGGTCATGGTACGTTTCTTTATTTCTCCTTTCCACATTTTTGAATTAATTCTATTAGAAAAAGAAGGAAAAAAAACATGAACTGGGCAGGTGGCAGGAGGTGATGGCACTGTCGGTTGGGTACTGGGAAGTCTTGCAGAACTTCACAAACAAGATCGCAGCCCTGTTCTTCCAGTTGGAATTATCCCTCTGGGTACTGGAAATGACCTCTCCAGGAGTTTTGGCTGGGTGAGTTTTTGTAATTTCAACACAAATTTTTGCATGAAGTGATCATCGTTTATTTCAATGGACATTGGTTTTTGGTTTGATCAGGGAGGTTCATTTCCTTTTGCCTGGAAATACGCCGTTAAAAGATCTCTCCACAGAGCTACCTCTGGCCAAGTTCGTTCTTTGGATAGGTGGGAATTCCAATTCTTTCAATGGCATTGCTTCTTGAATAGTAAAAGGCGTTTTTGAGTTGTTTCAAACTTGCTATTCTCACGGCGCAGTTGGCATGTTTCGCTGTCCATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTCTGAAGCAGGTTACACAAGAAAGTGATCTGAATGAGCCTCCTGTTCATGTAAATTATTTACATTTTCACACTACAAATTTTGCATTATATTCTCATTTCAGTCACGATTCAAAATCCATAATCCCATGGCTGCAGGATGACAAAGGAGATTTACCAACAAAACTGGCTTGCTATGAAGGAGTGTTTTACAATTACTTTAGTATAGGTAGGCGCTGTACTCACTTGTGTCTGCATGATCAATCAAACTGTAGAGTATTCGTTCACAGGTTTAATTAATTGATTCTACTATTTCACAAATGCAGGAATGGATGCTCAAGTTGCTTACGGCTTCCACCATTTGCGTAACAAAAGACCTTACCTTGCTCAAGGTCCAGTGACAAATAAGGTCCAGACCATCTGTTTAGCCATTGACGAAAGAATTGCATTCAGGACTAGTGGTTGTTTTGTTGTTTAATAAGCTTACACGTGCTTTCTTTTGTTTGTGACAGCTGATTTACTCTGGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAAAGATCCAAATTTGAGGTGATAATATTTATATTTAATGAAAACCCAGTAGAATACATCGGTTTTCTTCCAGTTTCTAAGCACAGAAAGCTTCTCTGGTTGTAGGGGCCTTAAGAACATCCTTAGAATACACATCAAAAAGGTCAATTGCTCGGACTGGGAAAAGATTCCAGTTCCTTCCAGGTAGAATTACTCTAGTAACAGTACAGTAACCATGAAACTATGCTTTTATGTAGAAATTTAAGCTTGTTGAGGACATTAATTGATGTTCTTGTTTCGATTGGCTGTAGTGTTAGGTCTATTGTTGCTTTAAACCTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCCCAAGCCAGAGTACTTGGAGAAGGTACCCACTAAAATTTCCCTTGATGAACAAGTTGTTGTTCTGTAATTTGCTTCTTTTTGGCATTTGATATTTATTTGGTAGCAGAGAGGGTTTGTGGAGGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGGCTAAAACAAGGATGGCACACATCATTTGTTATGGTGGATATAATCTCTGCCAAACACATTGCCCAGGTACTTCAAATTAAACGCATTCAGACATAGTTCTTCTCTTGAAGTTGAATCTAACTCCACGATTCTAGGGTGTTTGATTGAACTGTTGCATAATATTTTCAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGGAAGAAGGCATGTCTGCAGATGGATGGCGAGCCATGGAAGCAGCAAATAAGCAAGGACTCCTCGACGTTCGTTGAAATCAAGAGGGTTCCTTTCCAATCACTAATGATCAACGGGGACTAA

mRNA sequence

ATGGATTCCACAGCCGACGACGTTACTATTGCTGCTCGCTCCTCCTTAATCGACTCCTTGAGGGGCTGTGGCTTGTCCGGGATCCCAATCGACAAGGCCCACCTCAAGGCCAACCTCATCATGCCGCAGTATCTCCGCTTGGCCCTGCGGGATTCAATTCGCTTCAAAGATCCCACCGCCAAAATCACTACGCCGGAGAACGTCCTGCCGCCGGAGGCTCCTCTGGTCGTCTTCATTAATCCGCGTAGCGGTGGACGCCATGGCCAGAAGCTCAAGCAGCGCCTCCAGCACTTAATCAGCGACGAACAGGTTTTCGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTACGGACTGCGTTGCCTGGAAATCTTGTCTGATCTTGGCGACGCTTGTGCCAAACAAACTCGTCAAAAGTTAAGGGTCATGGTGGCAGGAGGTGATGGCACTGTCGGTTGGGTACTGGGAAGTCTTGCAGAACTTCACAAACAAGATCGCAGCCCTGTTCTTCCAGTTGGAATTATCCCTCTGGGTACTGGAAATGACCTCTCCAGGAGTTTTGGCTGGGGAGGTTCATTTCCTTTTGCCTGGAAATACGCCGTTAAAAGATCTCTCCACAGAGCTACCTCTGGCCAAGTTCGTTCTTTGGATAGTTGGCATGTTTCGCTGTCCATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTCTGAAGCAGGTTACACAAGAAAGTGATCTGAATGAGCCTCCTGTTCATGATGACAAAGGAGATTTACCAACAAAACTGGCTTGCTATGAAGGAGTGTTTTACAATTACTTTAGTATAGGAATGGATGCTCAAGTTGCTTACGGCTTCCACCATTTGCGTAACAAAAGACCTTACCTTGCTCAAGGTCCAGTGACAAATAAGCTGATTTACTCTGGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAAAGATCCAAATTTGAGGGGCCTTAAGAACATCCTTAGAATACACATCAAAAAGGTCAATTGCTCGGACTGGGAAAAGATTCCAGTTCCTTCCAGTGTTAGGTCTATTGTTGCTTTAAACCTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCCCAAGCCAGAGTACTTGGAGAAGAGAGGGTTTGTGGAGGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGGCTAAAACAAGGATGGCACACATCATTTGTTATGGTGGATATAATCTCTGCCAAACACATTGCCCAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGGAAGAAGGCATGTCTGCAGATGGATGGCGAGCCATGGAAGCAGCAAATAAGCAAGGACTCCTCGACGTTCGTTGAAATCAAGAGGGTTCCTTTCCAATCACTAATGATCAACGGGGACTAA

Coding sequence (CDS)

ATGGATTCCACAGCCGACGACGTTACTATTGCTGCTCGCTCCTCCTTAATCGACTCCTTGAGGGGCTGTGGCTTGTCCGGGATCCCAATCGACAAGGCCCACCTCAAGGCCAACCTCATCATGCCGCAGTATCTCCGCTTGGCCCTGCGGGATTCAATTCGCTTCAAAGATCCCACCGCCAAAATCACTACGCCGGAGAACGTCCTGCCGCCGGAGGCTCCTCTGGTCGTCTTCATTAATCCGCGTAGCGGTGGACGCCATGGCCAGAAGCTCAAGCAGCGCCTCCAGCACTTAATCAGCGACGAACAGGTTTTCGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTACGGACTGCGTTGCCTGGAAATCTTGTCTGATCTTGGCGACGCTTGTGCCAAACAAACTCGTCAAAAGTTAAGGGTCATGGTGGCAGGAGGTGATGGCACTGTCGGTTGGGTACTGGGAAGTCTTGCAGAACTTCACAAACAAGATCGCAGCCCTGTTCTTCCAGTTGGAATTATCCCTCTGGGTACTGGAAATGACCTCTCCAGGAGTTTTGGCTGGGGAGGTTCATTTCCTTTTGCCTGGAAATACGCCGTTAAAAGATCTCTCCACAGAGCTACCTCTGGCCAAGTTCGTTCTTTGGATAGTTGGCATGTTTCGCTGTCCATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTCTGAAGCAGGTTACACAAGAAAGTGATCTGAATGAGCCTCCTGTTCATGATGACAAAGGAGATTTACCAACAAAACTGGCTTGCTATGAAGGAGTGTTTTACAATTACTTTAGTATAGGAATGGATGCTCAAGTTGCTTACGGCTTCCACCATTTGCGTAACAAAAGACCTTACCTTGCTCAAGGTCCAGTGACAAATAAGCTGATTTACTCTGGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAAAGATCCAAATTTGAGGGGCCTTAAGAACATCCTTAGAATACACATCAAAAAGGTCAATTGCTCGGACTGGGAAAAGATTCCAGTTCCTTCCAGTGTTAGGTCTATTGTTGCTTTAAACCTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCCCAAGCCAGAGTACTTGGAGAAGAGAGGGTTTGTGGAGGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGGCTAAAACAAGGATGGCACACATCATTTGTTATGGTGGATATAATCTCTGCCAAACACATTGCCCAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGGAAGAAGGCATGTCTGCAGATGGATGGCGAGCCATGGAAGCAGCAAATAAGCAAGGACTCCTCGACGTTCGTTGAAATCAAGAGGGTTCCTTTCCAATCACTAATGATCAACGGGGACTAA

Protein sequence

MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD

Homology

[Close]

BLAST of Moc06g05250 vs. NCBI nr
Match: XP_022134720.1 (diacylglycerol kinase 7-like [Momordica charantia])

HSP 1 Score: 982.6 bits (2539), Expect = 1.2e-282
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0


Query: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60
           MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA
Sbjct: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60

Query: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120
           KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL
Sbjct: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120

Query: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180
           RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT
Sbjct: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180

Query: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240
           GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ
Sbjct: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240

Query: 241 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300
           VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 241 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300

Query: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360
           VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA
Sbjct: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360

Query: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420
           LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420

Query: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD
Sbjct: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 474

[Close]

BLAST of Moc06g05250 vs. NCBI nr
Match: XP_023516209.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 848.6 bits (2191), Expect = 2.6e-242
Identity = 411/474 (86.71%), Postives = 440/474 (92.83%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTAGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 305
           +T+ESDLNE  + D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 249 LTEESDLNEAKL-DEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 308

Query: 306 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 365
           +TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV 
Sbjct: 309 LTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVT 368

Query: 366 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 425
           LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 369 LNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 428

Query: 426 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           AQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 AQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of Moc06g05250 vs. NCBI nr
Match: KAG6589732.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 847.0 bits (2187), Expect = 7.6e-242
Identity = 410/474 (86.50%), Postives = 439/474 (92.62%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT   +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 305
           +T+ESDLNE  + D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 249 LTEESDLNEAKL-DEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 308

Query: 306 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 365
           +TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV 
Sbjct: 309 LTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVT 368

Query: 366 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 425
           LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 369 LNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 428

Query: 426 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           AQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 AQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of Moc06g05250 vs. NCBI nr
Match: XP_023516208.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 846.3 bits (2185), Expect = 1.3e-241
Identity = 411/475 (86.53%), Postives = 439/475 (92.42%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTAGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPPVH-DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 305
           +T+ESDLNE     D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG
Sbjct: 249 LTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 308

Query: 306 PVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIV 365
           P+TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV
Sbjct: 309 PLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIV 368

Query: 366 ALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 425
            LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH
Sbjct: 369 TLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 428

Query: 426 IAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           IAQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 482

[Close]

BLAST of Moc06g05250 vs. NCBI nr
Match: XP_022921758.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 845.1 bits (2182), Expect = 2.9e-241
Identity = 411/474 (86.71%), Postives = 440/474 (92.83%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPT--AKIT 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT  A  +
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  TP-ENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
            P +N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 305
           +T+ESDLNE  + D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 249 LTEESDLNEAKL-DEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 308

Query: 306 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 365
           +TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV 
Sbjct: 309 LTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVT 368

Query: 366 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 425
           LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 369 LNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 428

Query: 426 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           AQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 AQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of Moc06g05250 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 3.9e-188
Identity = 311/465 (66.88%), Postives = 373/465 (80.22%), Query Frame = 0


Query: 19  SLRGCG---LSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA---KITTPENV---L 78
           ++RGCG   L+ + IDK  L+  L MP+YLR+A+RD I+ KD T     +  P      +
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 79  PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDL 138
            P AP+VVFINP+SGGRHG  LK+RLQ L+++EQVFDL +VKPHEFV+YGL CL+ L+  
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 139 GDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFG 198
           GD CA++ R+K+R+MVAGGDGTVGWVLG L ELHK  +S + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 199 WGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNE 258
           WGGSFPFAW+ A+KR+LHRAT G +  LDSW + +SMP+GE V+PP+SLK   +E+ L++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 259 PPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSG 318
               D  GD+P K   YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS 
Sbjct: 270 --ALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSS 329

Query: 319 YSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASG 378
           YSCTQGWF T C  +P LRGL+NI++IHIKK NCS+WE+I VP SVRSIV LNL+NY SG
Sbjct: 330 YSCTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSG 389

Query: 379 RNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIE 438
           R+PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR E
Sbjct: 390 RHPWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFE 449

Query: 439 IRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           +RGG  K A LQMDGEPWKQ +  D STFVEIK+VPFQSLMING+
Sbjct: 450 LRGGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

[Close]

BLAST of Moc06g05250 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 1.9e-182
Identity = 308/479 (64.30%), Postives = 376/479 (78.50%), Query Frame = 0


Query: 10  IAARSSLIDS--LRGCGLSG---IPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT 69
           +A+R S  DS  +RGCGL+    + +DK  L+  L+MP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSA---I 75

Query: 70  PENVL---------PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEF 129
           P+++L          P AP+VVFINP SGGRHG  LK+RLQ L+S+EQVFDL +VKPHEF
Sbjct: 76  PDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEF 135

Query: 130 VQYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGI 189
           V+YGL CLE ++  GD CAK+ R +LR+MVAGGDGTVGWVLG L EL+K  +S + PVG+
Sbjct: 136 VRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGV 195

Query: 190 IPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPP 249
           IPLGTGNDLSRSFGWGGSFPFAW+ AVKR+LHRA+ G V  LDSW + +SMP+GE V+PP
Sbjct: 196 IPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPP 255

Query: 250 HSLKQVTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPY 309
           +SLK   +E++L++    D   D P     YEGVFYNY SIGMDAQVAYGFHHLRN +PY
Sbjct: 256 YSLKP-AEENELDQG--LDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPY 315

Query: 310 LAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSV 369
           LAQGP++NK+IYS + C+QGWF T C  DP LRGL+NI++IHIKKVNCS WE+I VP +V
Sbjct: 316 LAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNV 375

Query: 370 RSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDII 429
           RSIVALNLH+Y SG +PWG  KP+YLEKRGFVEAH DDGL+EIFG KQGWH SFVM ++I
Sbjct: 376 RSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELI 435

Query: 430 SAKHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           SAKHIAQAAA+R E+RGG  + A LQMDGEPWKQ +S + STFVEIK+VP+QSLMIN +
Sbjct: 436 SAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

[Close]

BLAST of Moc06g05250 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 5.1e-180
Identity = 294/456 (64.47%), Postives = 369/456 (80.92%), Query Frame = 0


Query: 25  LSGIPIDKAHLKANLIMPQYLRLALRDSIRFK----DPTAKITTP--ENVLPPEAPLVVF 84
           L+ I + KA L+  +++PQYLR+A+RD I  K    D ++ +  P   N L PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 85  INPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLGDACAKQTR 144
           +NP+SGGR G  +K+RLQ+LIS+EQV+DL +VKP+EF++YGL CLE  +  GD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 145 QKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 204
           +K+R++VAGGDGTVGWVLG L EL+ Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 205 KYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNEPPVHDDKGD 264
           K A+KR+LHRA+   +  LDSW++ ++MP+GE V+PP+SLK  TQE  +++    + +G+
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLK-ATQECYIDQN--LEIEGE 274

Query: 265 LPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFF 324
           +P     YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYSGY C+QGWF 
Sbjct: 275 IPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFL 334

Query: 325 TSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKP 384
           T C  DP LRGLKNI+ +HIKK++ S+WEK+PVP SVR++VALNLH+Y SGRNPWG  K 
Sbjct: 335 THCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQ 394

Query: 385 EYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKA 444
           +YLEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A
Sbjct: 395 DYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDA 454

Query: 445 CLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
            +QMDGEPWKQ +++D STFV+IKRVP QSL++ GD
Sbjct: 455 FMQMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

[Close]

BLAST of Moc06g05250 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 3.0e-71
Identity = 155/426 (36.38%), Postives = 224/426 (52.58%), Query Frame = 0


Query: 57  DPTAKITTPENVLPPEA-----------PLVVFINPRSGGRHGQKLKQRLQHLISDEQVF 116
           +P      P+ +L P++           P+VVFIN RSGG+ G  L +  + L++  QVF
Sbjct: 17  EPLTDYCIPDYILNPDSEQVLVDQAPCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVF 76

Query: 117 DLLDVKPHEFVQYGLRC-LEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHK 176
           DL +  P E V + L C  E L   GD  A Q +  LR++VAGGDGT  W+LG +++L K
Sbjct: 77  DLSEEAP-EKVLHRLYCNFEKLKSNGDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDL-K 136

Query: 177 QDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSL 236
               P  P+  +PLGTGN+L  SFGWG   P   + AVK  L +    +  ++DSWH+ +
Sbjct: 137 LSHPP--PIATVPLGTGNNLPFSFGWGKKNPTTDQEAVKSFLGQVKKAREMNIDSWHIIM 196

Query: 237 SMPAGEK--------VEPPHSL---KQVTQESDLNEPPVHDDKGDLPTKLACYEGVFYNY 296
            M A ++        +E PHSL    +V+    LN    H            Y G F+NY
Sbjct: 197 RMRAPQEGPCEPIAPLELPHSLHAFHRVSGSDSLNMEGYH-----------TYRGGFWNY 256

Query: 297 FSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNI 356
           FS+GMDAQV+Y FH  R + P   +  +TN+  Y+     QGWF  S +  P+ R +  +
Sbjct: 257 FSMGMDAQVSYEFHSERKRNPEKFKNQLTNQSTYAKLGLKQGWFAASLT-HPSSRNIAQL 316

Query: 357 LRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADD 416
            ++ I K     WE++ +P S+RSIV LNL +++ G NPWG P    ++ R       DD
Sbjct: 317 AKVRIMKRPGGQWEELKIPRSIRSIVCLNLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDD 376

Query: 417 GLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISK 460
           GL+E+ G +  WH   ++        +AQA  IR E   G  +   +++DGEPWKQ + K
Sbjct: 377 GLIEVVGFRDAWHGLVLLAPNGHGTRLAQAHRIRFEFHKGAAEHTFMRIDGEPWKQPLPK 426

[Close]

BLAST of Moc06g05250 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 8.6e-71
Identity = 149/412 (36.17%), Postives = 225/412 (54.61%), Query Frame = 0


Query: 57  DPTAKITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFV 116
           +P A+I+       PE P++VFIN +SGG+ G +L    + L++D+QVFDL    P + +
Sbjct: 33  EPEAQISC---TTAPENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVETPDKVL 92

Query: 117 QYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGII 176
           Q     LE L D  D+ A + R KL+++VAGGDGT GW+LG +++L+  +     P+  +
Sbjct: 93  QRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSNPP---PIATV 152

Query: 177 PLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEK----- 236
           PLGTGN+L  +FGWG   P   + +V+  L +  + +   +D+W + + M   ++     
Sbjct: 153 PLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKEGSCDI 212

Query: 237 -VEPPHSLKQVTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLR 296
            ++ PHSL ++      N    H            Y G F+NYFS+GMDAQV+Y FH  R
Sbjct: 213 TLKLPHSLPRIFPSDQENMEGYH-----------TYRGGFWNYFSLGMDAQVSYAFHSQR 272

Query: 297 NKRPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIP 356
              P   +  + N+  Y   SCTQGWFF S    P+ + +  + +I I   N   W  + 
Sbjct: 273 KLHPERFKNQLVNQSTYLKLSCTQGWFFASLF-HPSSQNIAKLAKIQICDRN-GQWNDLH 332

Query: 357 VPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFV 416
           +P S+RSIV LNL +++ G NPWG P P+    R       DDGL+EI G +  WH   +
Sbjct: 333 IPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLIL 392

Query: 417 MVDIISAKHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQI-SKDSSTFVEI 462
           +        +AQA  +R+E + G  K A +++DGEPWKQ + S D +  VEI
Sbjct: 393 LSPNGHGTRLAQANRVRLEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

[Close]

BLAST of Moc06g05250 vs. ExPASy TrEMBL
Match: A0A6J1BZ41 (Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111006923 PE=3 SV=1)

HSP 1 Score: 982.6 bits (2539), Expect = 5.6e-283
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0


Query: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60
           MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA
Sbjct: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60

Query: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120
           KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL
Sbjct: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120

Query: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180
           RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT
Sbjct: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180

Query: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240
           GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ
Sbjct: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240

Query: 241 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300
           VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 241 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300

Query: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360
           VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA
Sbjct: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360

Query: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420
           LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420

Query: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD
Sbjct: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 474

[Close]

BLAST of Moc06g05250 vs. ExPASy TrEMBL
Match: A0A6J1E4R5 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.4e-241
Identity = 411/474 (86.71%), Postives = 440/474 (92.83%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPT--AKIT 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT  A  +
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  TP-ENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
            P +N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 305
           +T+ESDLNE  + D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 249 LTEESDLNEAKL-DEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 308

Query: 306 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 365
           +TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV 
Sbjct: 309 LTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVT 368

Query: 366 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 425
           LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 369 LNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 428

Query: 426 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           AQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 AQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of Moc06g05250 vs. ExPASy TrEMBL
Match: A0A6J1E1C6 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 7.0e-241
Identity = 411/475 (86.53%), Postives = 439/475 (92.42%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPT--AKIT 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT  A  +
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  TP-ENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
            P +N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPPVH-DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 305
           +T+ESDLNE     D+KGDLPTKLA YEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG
Sbjct: 249 LTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 308

Query: 306 PVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIV 365
           P+TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV
Sbjct: 309 PLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIV 368

Query: 366 ALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 425
            LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH
Sbjct: 369 TLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 428

Query: 426 IAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           IAQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 482

[Close]

BLAST of Moc06g05250 vs. ExPASy TrEMBL
Match: A0A6J1JHE1 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.0e-239
Identity = 405/474 (85.44%), Postives = 437/474 (92.19%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLA+RDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKDPTAGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  LPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+ QDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGE+VEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERVEPPHSLK- 248

Query: 246 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 305
           +T+ESDLNE  + D+KGDLPTKLA Y GVFYNYFSIGMDAQVAYGFHHLRNKRP LA+GP
Sbjct: 249 LTEESDLNEAKL-DEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNKRPKLARGP 308

Query: 306 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 365
           +TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV 
Sbjct: 309 LTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVT 368

Query: 366 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 425
           LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 369 LNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 428

Query: 426 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           AQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 AQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of Moc06g05250 vs. ExPASy TrEMBL
Match: A0A6J1JJR6 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.5e-238
Identity = 405/475 (85.26%), Postives = 436/475 (91.79%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLA+RDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKDPTAGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  LPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+ QDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGE+VEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERVEPPHSLK- 248

Query: 246 VTQESDLNEPPVH-DDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 305
           +T+ESDLNE     D+KGDLPTKLA Y GVFYNYFSIGMDAQVAYGFHHLRNKRP LA+G
Sbjct: 249 LTEESDLNEAVRKLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNKRPKLARG 308

Query: 306 PVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIV 365
           P+TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV
Sbjct: 309 PLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIV 368

Query: 366 ALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 425
            LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH
Sbjct: 369 TLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 428

Query: 426 IAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           IAQAAAIRIEIRGGKRKKA LQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 482

[Close]

BLAST of Moc06g05250 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 659.1 bits (1699), Expect = 2.7e-189
Identity = 311/465 (66.88%), Postives = 373/465 (80.22%), Query Frame = 0


Query: 19  SLRGCG---LSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA---KITTPENV---L 78
           ++RGCG   L+ + IDK  L+  L MP+YLR+A+RD I+ KD T     +  P      +
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 79  PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDL 138
            P AP+VVFINP+SGGRHG  LK+RLQ L+++EQVFDL +VKPHEFV+YGL CL+ L+  
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 139 GDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFG 198
           GD CA++ R+K+R+MVAGGDGTVGWVLG L ELHK  +S + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 199 WGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNE 258
           WGGSFPFAW+ A+KR+LHRAT G +  LDSW + +SMP+GE V+PP+SLK   +E+ L++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 259 PPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSG 318
               D  GD+P K   YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS 
Sbjct: 270 --ALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSS 329

Query: 319 YSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASG 378
           YSCTQGWF T C  +P LRGL+NI++IHIKK NCS+WE+I VP SVRSIV LNL+NY SG
Sbjct: 330 YSCTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSG 389

Query: 379 RNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIE 438
           R+PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR E
Sbjct: 390 RHPWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFE 449

Query: 439 IRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           +RGG  K A LQMDGEPWKQ +  D STFVEIK+VPFQSLMING+
Sbjct: 450 LRGGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

[Close]

BLAST of Moc06g05250 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.3e-183
Identity = 308/479 (64.30%), Postives = 376/479 (78.50%), Query Frame = 0


Query: 10  IAARSSLIDS--LRGCGLSG---IPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT 69
           +A+R S  DS  +RGCGL+    + +DK  L+  L+MP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSA---I 75

Query: 70  PENVL---------PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEF 129
           P+++L          P AP+VVFINP SGGRHG  LK+RLQ L+S+EQVFDL +VKPHEF
Sbjct: 76  PDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEF 135

Query: 130 VQYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGI 189
           V+YGL CLE ++  GD CAK+ R +LR+MVAGGDGTVGWVLG L EL+K  +S + PVG+
Sbjct: 136 VRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGV 195

Query: 190 IPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPP 249
           IPLGTGNDLSRSFGWGGSFPFAW+ AVKR+LHRA+ G V  LDSW + +SMP+GE V+PP
Sbjct: 196 IPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPP 255

Query: 250 HSLKQVTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPY 309
           +SLK   +E++L++    D   D P     YEGVFYNY SIGMDAQVAYGFHHLRN +PY
Sbjct: 256 YSLKP-AEENELDQG--LDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPY 315

Query: 310 LAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSV 369
           LAQGP++NK+IYS + C+QGWF T C  DP LRGL+NI++IHIKKVNCS WE+I VP +V
Sbjct: 316 LAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNV 375

Query: 370 RSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDII 429
           RSIVALNLH+Y SG +PWG  KP+YLEKRGFVEAH DDGL+EIFG KQGWH SFVM ++I
Sbjct: 376 RSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELI 435

Query: 430 SAKHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
           SAKHIAQAAA+R E+RGG  + A LQMDGEPWKQ +S + STFVEIK+VP+QSLMIN +
Sbjct: 436 SAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

[Close]

BLAST of Moc06g05250 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 632.1 bits (1629), Expect = 3.6e-181
Identity = 294/456 (64.47%), Postives = 369/456 (80.92%), Query Frame = 0


Query: 25  LSGIPIDKAHLKANLIMPQYLRLALRDSIRFK----DPTAKITTP--ENVLPPEAPLVVF 84
           L+ I + KA L+  +++PQYLR+A+RD I  K    D ++ +  P   N L PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 85  INPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLGDACAKQTR 144
           +NP+SGGR G  +K+RLQ+LIS+EQV+DL +VKP+EF++YGL CLE  +  GD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 145 QKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 204
           +K+R++VAGGDGTVGWVLG L EL+ Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 205 KYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNEPPVHDDKGD 264
           K A+KR+LHRA+   +  LDSW++ ++MP+GE V+PP+SLK  TQE  +++    + +G+
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLK-ATQECYIDQN--LEIEGE 274

Query: 265 LPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFF 324
           +P     YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYSGY C+QGWF 
Sbjct: 275 IPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFL 334

Query: 325 TSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKP 384
           T C  DP LRGLKNI+ +HIKK++ S+WEK+PVP SVR++VALNLH+Y SGRNPWG  K 
Sbjct: 335 THCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQ 394

Query: 385 EYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKA 444
           +YLEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A
Sbjct: 395 DYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDA 454

Query: 445 CLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 475
            +QMDGEPWKQ +++D STFV+IKRVP QSL++ GD
Sbjct: 455 FMQMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

[Close]

BLAST of Moc06g05250 vs. TAIR 10
Match: AT4G28130.1 (diacylglycerol kinase 6 )

HSP 1 Score: 269.2 bits (687), Expect = 6.1e-72
Identity = 149/412 (36.17%), Postives = 225/412 (54.61%), Query Frame = 0


Query: 57  DPTAKITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFV 116
           +P A+I+       PE P++VFIN +SGG+ G +L    + L++D+QVFDL    P + +
Sbjct: 33  EPEAQISC---TTAPENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVETPDKVL 92

Query: 117 QYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGII 176
           Q     LE L D  D+ A + R KL+++VAGGDGT GW+LG +++L+  +     P+  +
Sbjct: 93  QRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSNPP---PIATV 152

Query: 177 PLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEK----- 236
           PLGTGN+L  +FGWG   P   + +V+  L +  + +   +D+W + + M   ++     
Sbjct: 153 PLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKEGSCDI 212

Query: 237 -VEPPHSLKQVTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLR 296
            ++ PHSL ++      N    H            Y G F+NYFS+GMDAQV+Y FH  R
Sbjct: 213 TLKLPHSLPRIFPSDQENMEGYH-----------TYRGGFWNYFSLGMDAQVSYAFHSQR 272

Query: 297 NKRPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIP 356
              P   +  + N+  Y   SCTQGWFF S    P+ + +  + +I I   N   W  + 
Sbjct: 273 KLHPERFKNQLVNQSTYLKLSCTQGWFFASLF-HPSSQNIAKLAKIQICDRN-GQWNDLH 332

Query: 357 VPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFV 416
           +P S+RSIV LNL +++ G NPWG P P+    R       DDGL+EI G +  WH   +
Sbjct: 333 IPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLIL 392

Query: 417 MVDIISAKHIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQI-SKDSSTFVEI 462
           +        +AQA  +R+E + G  K A +++DGEPWKQ + S D +  VEI
Sbjct: 393 LSPNGHGTRLAQANRVRLEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

[Close]

BLAST of Moc06g05250 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 265.4 bits (677), Expect = 8.8e-71
Identity = 149/417 (35.73%), Postives = 220/417 (52.76%), Query Frame = 0


Query: 71  PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLG 130
           P +P++VFIN +SGG+ G +L    + L++  QVFDL    P + ++     LE L D  
Sbjct: 37  PASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD-- 96

Query: 131 DACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGW 190
           D  A+Q R+KL+++VAGGDGT GW+LG + +L K    P  P+  +PLGTGN+L  +FGW
Sbjct: 97  DDFARQIREKLKIIVAGGDGTAGWLLGVVCDL-KLSHPP--PIATVPLGTGNNLPFAFGW 156

Query: 191 GGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEK---------VEPPHSL--- 250
           G   P   + AV+  L +    +V  +D+WH+ + M   ++         +E PHSL   
Sbjct: 157 GKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSLHAF 216

Query: 251 KQVTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQ 310
            +V+   +LN+   H            + G F+NYFS+GMDAQ++Y FH  R   P   +
Sbjct: 217 HRVSPTDELNKEGCH-----------TFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFK 276

Query: 311 GPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSI 370
             + N+  Y    CTQGWF  S    P  R +  + ++ I   N   W+ + +P S+RSI
Sbjct: 277 NQLVNQSTYVKLGCTQGWFCASLF-HPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSI 336

Query: 371 VALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAK 430
           V LNL +++ G NPWG P P     RG      DDGL+E+ G +  WH   ++       
Sbjct: 337 VCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGT 396

Query: 431 HIAQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSST-FVEIKRVPFQSLMINGD 475
            +AQA  IR E   G      ++MDGEPWKQ +  D  T  VEI  +   +++   D
Sbjct: 397 RLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNMLATHD 435

The following BLAST results are available for this feature:

BLAST of Moc06g05250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134720.1	1.2e-282	100.00	diacylglycerol kinase 7-like [Momordica charantia]	[more]
XP_023516209.1	2.6e-242	86.71	diacylglycerol kinase 7-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAG6589732.1	7.6e-242	86.50	Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023516208.1	1.3e-241	86.53	diacylglycerol kinase 7-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022921758.1	2.9e-241	86.71	diacylglycerol kinase 7-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Moc06g05250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JQ95	3.9e-188	66.88	Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1	[more]
Q8VZG1	1.9e-182	64.30	Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1	[more]
Q1PDI2	5.1e-180	64.47	Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1	[more]
Q7XQT2	3.0e-71	36.38	Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV...	[more]
F4JKI3	8.6e-71	36.17	Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1	[more]

BLAST of Moc06g05250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZ41	5.6e-283	100.00	Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111006923 PE=3 SV=1	[more]
A0A6J1E4R5	1.4e-241	86.71	diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1	[more]
A0A6J1E1C6	7.0e-241	86.53	diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1	[more]
A0A6J1JHE1	5.0e-239	85.44	diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1	[more]
A0A6J1JJR6	2.5e-238	85.26	diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1	[more]

BLAST of Moc06g05250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30340.1	2.7e-189	66.88	diacylglycerol kinase 7	[more]
AT2G18730.1	1.3e-183	64.30	diacylglycerol kinase 3	[more]
AT5G57690.1	3.6e-181	64.47	diacylglycerol kinase 4	[more]
AT4G28130.1	6.1e-72	36.17	diacylglycerol kinase 6	[more]
AT2G20900.1	8.8e-71	35.73	diacylglycerol kinase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000756	Diacylglycerol kinase, accessory domain	SMART	SM00045	dagk_c4b_2	coord: 269..446 e-value: 1.6E-9 score: 37.9
IPR000756	Diacylglycerol kinase, accessory domain	PFAM	PF00609	DAGK_acc	coord: 269..446 e-value: 2.1E-42 score: 145.2
IPR001206	Diacylglycerol kinase, catalytic domain	SMART	SM00046	dagk_c4a_7	coord: 74..220 e-value: 7.0E-27 score: 105.3
IPR001206	Diacylglycerol kinase, catalytic domain	PFAM	PF00781	DAGK_cat	coord: 74..213 e-value: 4.4E-30 score: 103.9
IPR001206	Diacylglycerol kinase, catalytic domain	PROSITE	PS50146	DAGK	coord: 70..227 score: 29.788589
None	No IPR available	GENE3D	2.60.200.40		coord: 265..452 e-value: 1.5E-6 score: 30.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 228..254
None	No IPR available	PANTHER	PTHR11255:SF96	DIACYLGLYCEROL KINASE	coord: 3..473
IPR016961	Diacylglycerol kinase, plant	PIRSF	PIRSF030829	DAG_kinase_plant	coord: 2..474 e-value: 2.2E-209 score: 694.0
IPR017438	Inorganic polyphosphate/ATP-NAD kinase, N-terminal	GENE3D	3.40.50.10330		coord: 72..223 e-value: 3.0E-23 score: 83.8
IPR037607	Diacylglycerol kinase	PANTHER	PTHR11255	DIACYLGLYCEROL KINASE	coord: 3..473
IPR016064	NAD kinase/diacylglycerol kinase-like domain superfamily	SUPERFAMILY	111331	NAD kinase/diacylglycerol kinase-like	coord: 72..448

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc06g05250.1	Moc06g05250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0016310	phosphorylation
biological_process	GO:0007205	protein kinase C-activating G protein-coupled receptor signaling pathway
biological_process	GO:0007165	signal transduction
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004143	diacylglycerol kinase activity
molecular_function	GO:0003951	NAD+ kinase activity
molecular_function	GO:0016301	kinase activity