Moc06g01530 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc06g01530 vs. NCBI nr
Match: KAG6587481.1 (hypothetical protein SDJN03_16046, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 280.0 bits (715), Expect = 1.3e-71
Identity = 131/162 (80.86%), Postives = 141/162 (87.04%), Query Frame = 0

Query: 2   MTRTDAEEPPCEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGRW 61
           M    AEEPPCEADK+N+LLPTTSCGC R+SCFGSRR  A GPSWWERIRAS+VH EGRW
Sbjct: 1   MAYAHAEEPPCEADKSNSLLPTTSCGCSRLSCFGSRRTTAGGPSWWERIRASEVHNEGRW 60

Query: 62  WTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS--SGATRPGKFQYDPLSYAMNFDEG 121
           W RGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS  + A RPGKFQYDPLSYAMNFDEG
Sbjct: 61  WARGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSGATAAARPGKFQYDPLSYAMNFDEG 120

Query: 122 SREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKD 162
           S +IGELDDDIDDF+GFRNFS+RYASIP  +K+ G  V GKD
Sbjct: 121 SGQIGELDDDIDDFSGFRNFSARYASIPGSLKTAGTTVLGKD 162

BLAST of Moc06g01530 vs. NCBI nr
Match: XP_022928159.1 (uncharacterized protein LOC111435061 [Cucurbita moschata] >XP_023530985.1 uncharacterized protein LOC111793374 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 276.9 bits (707), Expect = 1.1e-70
Identity = 132/163 (80.98%), Postives = 142/163 (87.12%), Query Frame = 0

Query: 2   MTRTDAEEPP-CEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGR 61
           M    AEEPP CEADK+N+LLPTTSCGC R+SCFGSRR AA GPSWWERIRAS+VH EGR
Sbjct: 1   MAYAHAEEPPSCEADKSNSLLPTTSCGCSRLSCFGSRRTAAGGPSWWERIRASEVHNEGR 60

Query: 62  WWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS--SGATRPGKFQYDPLSYAMNFDE 121
           WW RGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS  + A RPGKFQYDPLSYAMNFDE
Sbjct: 61  WWARGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSGATAAARPGKFQYDPLSYAMNFDE 120

Query: 122 GSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKD 162
           GS +IGELDDDIDDF+GFRNFS+RYASIP  +K+ G  V GKD
Sbjct: 121 GSGQIGELDDDIDDFSGFRNFSARYASIPGSLKTAGTTVLGKD 163

BLAST of Moc06g01530 vs. NCBI nr
Match: XP_023003975.1 (uncharacterized protein LOC111497423 [Cucurbita maxima])

HSP 1 Score: 276.9 bits (707), Expect = 1.1e-70
Identity = 132/163 (80.98%), Postives = 142/163 (87.12%), Query Frame = 0

Query: 2   MTRTDAEEPP-CEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGR 61
           M    AEEPP CEADK+N+LLPTTSC C R+SCFGSRR AA GPSWWERIRAS+VH EGR
Sbjct: 1   MAYAHAEEPPSCEADKSNSLLPTTSCSCSRLSCFGSRRTAAGGPSWWERIRASEVHNEGR 60

Query: 62  WWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS--SGATRPGKFQYDPLSYAMNFDE 121
           WW RGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS  + A RPGKFQYDPLSYAMNFDE
Sbjct: 61  WWARGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSGATAAARPGKFQYDPLSYAMNFDE 120

Query: 122 GSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKD 162
           GS +IGELDDDIDDF+GFRNFS+RYASIP  +K+GG  V GKD
Sbjct: 121 GSGQIGELDDDIDDFSGFRNFSARYASIPGSLKTGGTTVLGKD 163

BLAST of Moc06g01530 vs. NCBI nr
Match: XP_038878924.1 (uncharacterized protein LOC120071012 [Benincasa hispida])

HSP 1 Score: 276.6 bits (706), Expect = 1.5e-70
Identity = 133/166 (80.12%), Postives = 143/166 (86.14%), Query Frame = 0

Query: 2   MTRTDA-EEPPCEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGR 61
           M  TD  EE PCEADK+N+LLPT  CGCFR+SCFGSRR A  GPSWWER+RASQVH EGR
Sbjct: 1   MAFTDVEEESPCEADKSNSLLPTARCGCFRLSCFGSRRVATVGPSWWERLRASQVHSEGR 60

Query: 62  WWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS---SGATRPGKFQYDPLSYAMNFD 121
           WW RGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS    G  R GKFQYDPLSYAMNFD
Sbjct: 61  WWARGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSGGGGGGVRAGKFQYDPLSYAMNFD 120

Query: 122 EGSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKDVS 164
           EGSR+IGELDDDIDDFNG+RNFS+RYASIPAP+K+GG  +  KDVS
Sbjct: 121 EGSRQIGELDDDIDDFNGYRNFSARYASIPAPLKTGGTII--KDVS 164

BLAST of Moc06g01530 vs. NCBI nr
Match: KAG7021468.1 (putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 275.4 bits (703), Expect = 3.3e-70
Identity = 130/158 (82.28%), Postives = 141/158 (89.24%), Query Frame = 0

Query: 7   AEEPP-CEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGRWWTRG 66
           +EEPP CEADK+N+LLPTTSCGC R+SCFGSRR AA GPSWWERIRAS+VH EGRWW RG
Sbjct: 161 SEEPPSCEADKSNSLLPTTSCGCSRLSCFGSRRTAAGGPSWWERIRASEVHNEGRWWARG 220

Query: 67  VRVLLKLREWSEIVAGPRWKTFIRRFNRNRS--SGATRPGKFQYDPLSYAMNFDEGSREI 126
           VRVLLKLREWSEIVAGPRWKTFIRRFNRNRS  + A RPGKFQYDPLSYAMNFDEGS +I
Sbjct: 221 VRVLLKLREWSEIVAGPRWKTFIRRFNRNRSGATAAARPGKFQYDPLSYAMNFDEGSGQI 280

Query: 127 GELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKD 162
           GELDDDIDDF+GFRNFS+RYASIP  +K+ G  V GKD
Sbjct: 281 GELDDDIDDFSGFRNFSARYASIPGSLKTAGTTVLGKD 318

BLAST of Moc06g01530 vs. ExPASy TrEMBL
Match: A0A6J1KTA4 (uncharacterized protein LOC111497423 OS=Cucurbita maxima OX=3661 GN=LOC111497423 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 5.4e-71
Identity = 132/163 (80.98%), Postives = 142/163 (87.12%), Query Frame = 0

Query: 2   MTRTDAEEPP-CEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGR 61
           M    AEEPP CEADK+N+LLPTTSC C R+SCFGSRR AA GPSWWERIRAS+VH EGR
Sbjct: 1   MAYAHAEEPPSCEADKSNSLLPTTSCSCSRLSCFGSRRTAAGGPSWWERIRASEVHNEGR 60

Query: 62  WWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS--SGATRPGKFQYDPLSYAMNFDE 121
           WW RGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS  + A RPGKFQYDPLSYAMNFDE
Sbjct: 61  WWARGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSGATAAARPGKFQYDPLSYAMNFDE 120

Query: 122 GSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKD 162
           GS +IGELDDDIDDF+GFRNFS+RYASIP  +K+GG  V GKD
Sbjct: 121 GSGQIGELDDDIDDFSGFRNFSARYASIPGSLKTGGTTVLGKD 163

BLAST of Moc06g01530 vs. ExPASy TrEMBL
Match: A0A6J1EK25 (uncharacterized protein LOC111435061 OS=Cucurbita moschata OX=3662 GN=LOC111435061 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 5.4e-71
Identity = 132/163 (80.98%), Postives = 142/163 (87.12%), Query Frame = 0

Query: 2   MTRTDAEEPP-CEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGR 61
           M    AEEPP CEADK+N+LLPTTSCGC R+SCFGSRR AA GPSWWERIRAS+VH EGR
Sbjct: 1   MAYAHAEEPPSCEADKSNSLLPTTSCGCSRLSCFGSRRTAAGGPSWWERIRASEVHNEGR 60

Query: 62  WWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS--SGATRPGKFQYDPLSYAMNFDE 121
           WW RGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS  + A RPGKFQYDPLSYAMNFDE
Sbjct: 61  WWARGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSGATAAARPGKFQYDPLSYAMNFDE 120

Query: 122 GSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKD 162
           GS +IGELDDDIDDF+GFRNFS+RYASIP  +K+ G  V GKD
Sbjct: 121 GSGQIGELDDDIDDFSGFRNFSARYASIPGSLKTAGTTVLGKD 163

BLAST of Moc06g01530 vs. ExPASy TrEMBL
Match: A0A6J1E188 (uncharacterized protein LOC111429865 OS=Cucurbita moschata OX=3662 GN=LOC111429865 PE=4 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 2.3e-69
Identity = 129/165 (78.18%), Postives = 142/165 (86.06%), Query Frame = 0

Query: 2   MTRTDAEE-PPCEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGR 61
           M   DAEE PPCE D +N+LLPTT CGCFR+SC   RR  A GPSWWERIRASQVH EGR
Sbjct: 1   MAYPDAEEPPPCEVDNSNSLLPTTRCGCFRLSCLEFRRAVAVGPSWWERIRASQVHSEGR 60

Query: 62  WWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS--SGATRPGKFQYDPLSYAMNFDE 121
           WW RGVRV+LK+REWSEIVAGPRWKTFIRRFNRNR+  +GA RPGKFQYDPLSYAMNFDE
Sbjct: 61  WWARGVRVILKIREWSEIVAGPRWKTFIRRFNRNRNGGAGAGRPGKFQYDPLSYAMNFDE 120

Query: 122 GSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKDVS 164
           GSR+IGELDDD DDFNG+RNFS+RYASIPAP+K+GG    GKD+S
Sbjct: 121 GSRQIGELDDDNDDFNGYRNFSARYASIPAPLKTGGTT--GKDIS 163

BLAST of Moc06g01530 vs. ExPASy TrEMBL
Match: A0A0A0LUB2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G005570 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 5.1e-69
Identity = 130/167 (77.84%), Postives = 139/167 (83.23%), Query Frame = 0

Query: 2   MTRTDAEE--PPCEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEG 61
           M  TD EE  P C+ADK+N+LLPT  CGCFR+SCFGSRR A  GPSWWERIRASQVH EG
Sbjct: 1   MAFTDVEEESPCCDADKSNSLLPTARCGCFRLSCFGSRRVATVGPSWWERIRASQVHNEG 60

Query: 62  RWWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSSG------------ATRPGKFQY 121
           RWWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRS G              R GKFQY
Sbjct: 61  RWWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSGGCGGCGSGGGGGSGVRAGKFQY 120

Query: 122 DPLSYAMNFDEGSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGG 155
           DPLSYAMNFDEGSR+IGELDDDIDDFNG+RNFS+RYASIPAP+K+GG
Sbjct: 121 DPLSYAMNFDEGSRQIGELDDDIDDFNGYRNFSARYASIPAPLKTGG 167

BLAST of Moc06g01530 vs. ExPASy TrEMBL
Match: A0A5D3BK38 (Putative Serine/arginine repetitive matrix protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001870 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.9e-68
Identity = 130/171 (76.02%), Postives = 141/171 (82.46%), Query Frame = 0

Query: 3   TRTDAEEPP-CEADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGRW 62
           T  D EE P CEADK+N+LLPT  CGCFR+SCFGSRR A  GPSWWERIRASQVH EGRW
Sbjct: 4   TDVDQEESPCCEADKSNSLLPTARCGCFRLSCFGSRRVATVGPSWWERIRASQVHSEGRW 63

Query: 63  WTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSSG---------ATRPGKFQYDPLSY 122
           W RGV+V+LKLREWSEIVAGPRWKTFIRRFNRNRS G           R GKFQYDPLSY
Sbjct: 64  WARGVKVILKLREWSEIVAGPRWKTFIRRFNRNRSGGCGGGGGGGSGVRAGKFQYDPLSY 123

Query: 123 AMNFDEGSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKDVS 164
           AMNFDEGSR+IGELDDD+DDFNG+RNFS+RYASIPAP+K+GG     KDVS
Sbjct: 124 AMNFDEGSRQIGELDDDMDDFNGYRNFSARYASIPAPLKTGGT----KDVS 170

BLAST of Moc06g01530 vs. TAIR 10
Match: AT3G48020.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 11 plant structures; EXPRESSED DURING: LP.04 four leaves visible, 4 anthesis; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G62865.1); Has 82 Blast hits to 82 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 82; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 110.2 bits (274), Expect = 1.7e-24
Identity = 64/147 (43.54%), Postives = 81/147 (55.10%), Query Frame = 0

Query: 13  EADKTNALLPTTSCGCFRVSCFGSRRPAAAGPSWWERIRASQVHGEGRWWTRGVRVLLKL 72
           E  + N LL  +SC    V             SWW+RI  +  H E RWW   VR  LK+
Sbjct: 2   EVGQANPLLGESSCCSTTVK-----------SSWWQRIHRNN-HQEPRWW---VRAFLKI 61

Query: 73  REWSEIVAGPRWKTFIRRFNRN--RSSGATRPGKFQYDPLSYAMNFDEGSREIGELDDDI 132
           REWSEIVAGPRWKTFIRRFNR+  R        KF+YDP+SY ++F++  ++    DDD 
Sbjct: 62  REWSEIVAGPRWKTFIRRFNRDPRRGQDWDDSDKFRYDPVSYTLSFEDEDKD----DDDE 121

Query: 133 DDFNGFRNFSSRYASIPAPMKSGGAAV 158
               G R+FS RYAS+P       A +
Sbjct: 122 AGVGGVRSFSMRYASVPVASGKSPAVI 129

BLAST of Moc06g01530 vs. TAIR 10
Match: AT5G62865.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G48020.1). )

HSP 1 Score: 109.4 bits (272), Expect = 2.9e-24
Identity = 67/131 (51.15%), Postives = 78/131 (59.54%), Query Frame = 0

Query: 26  CGCFRVSCFGSRRPAAAGPSWWERIRA------SQVHG-EGRWWTRGVRVLLKLREWSEI 85
           C CF  S   SR   A G S W RIR       S  HG E RWW   +R  LK+REWSEI
Sbjct: 20  CCCF-PSFRRSRSSTAVGYSSWGRIRTVDDSNHSGDHGDEPRWW---IRASLKIREWSEI 79

Query: 86  VAGPRWKTFIRRFNRNRSSGA--TRPGKFQYDPLSYAMNFDEGSREIGELDDDIDDFNGF 145
           VAGPRWKTFIRRFNR+   G       KFQYDPLSY++NFD+   E     D+     G 
Sbjct: 80  VAGPRWKTFIRRFNRDPRRGRDWDASEKFQYDPLSYSLNFDDDDEE-----DEYVGLGGL 139

Query: 146 RNFSSRYASIP 148
           R+FS+R+AS+P
Sbjct: 140 RSFSTRFASVP 141

BLAST of Moc06g01530 vs. TAIR 10
Match: AT3G01430.1 (BEST Arabidopsis thaliana protein match is: NHL domain-containing protein (TAIR:AT5G14890.1); Has 98 Blast hits to 98 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 98; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 107.5 bits (267), Expect = 1.1e-23
Identity = 65/180 (36.11%), Postives = 96/180 (53.33%), Query Frame = 0

Query: 10  PPCEADKTN----ALLPTTSCGCFRVSCFGSRRPAAAGPS-WWERI-RASQVHGEGRWWT 69
           P  E D T+    AL     C CF + C  S +P+  G S WW+RI    ++  + RWW 
Sbjct: 7   PIAEVDATDDMHEALFAKRGC-CFLMPCLASSQPSTRGGSVWWQRITTVDKLEPDERWWI 66

Query: 70  RGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSSG---------------------ATRP 129
           RG R   ++REWSE+VAGPRWKT+IRRF R+   G                     ++  
Sbjct: 67  RGWR---RMREWSELVAGPRWKTYIRRFGRSNCCGGGGGRVGNSSGGCGGGAMPNRSSDQ 126

Query: 130 GKFQYDPLSYAMNFDEGSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKDV 163
           GKF+YD LSY++NFD+G+ + G  DD+      +R++S R+A+   P+ +  +  F  D+
Sbjct: 127 GKFRYDQLSYSLNFDDGN-QTGHFDDEFP----YRDYSMRFAAPSLPVSTKCSIDFDSDI 177

BLAST of Moc06g01530 vs. TAIR 10
Match: AT5G14890.1 (NHL domain-containing protein )

HSP 1 Score: 106.7 bits (265), Expect = 1.9e-23
Identity = 64/166 (38.55%), Postives = 92/166 (55.42%), Query Frame = 0

Query: 10  PPCEADKTN----ALLPTTSCGCFRVSCFGSRRPAAA-GPSWWERIR-ASQVHGEGRWWT 69
           P  E D T+    A+     C CF + C GS +P+   G  WW+RIR   ++  + RWW 
Sbjct: 590 PISEMDATDEMHEAMFAKRGC-CFILPCLGSSQPSGPNGSVWWQRIRTVDKLEPDERWWV 649

Query: 70  RGVRVLLKLREWSEIVAGPRWKTFIRRFNRNR------SSGATRPG--KFQYDPLSYAMN 129
            G    +K+REWSEIVAGP+WKTFIRRF RN         G  RP    F+YD  SY++N
Sbjct: 650 SG---WMKMREWSEIVAGPKWKTFIRRFGRNHCCNGGIDGGCNRPEHVSFRYDSWSYSLN 709

Query: 130 FDEGSREIGELDDDIDDFNGFRNFSSRYASIPAPMKSGGAAVFGKD 162
           FD+G ++ G  +D+      +R++S R+A+   P+ +  +  F  D
Sbjct: 710 FDDG-KQTGHFEDEFP----YRDYSMRFAAPSLPVSTKCSIDFDND 746

BLAST of Moc06g01530 vs. TAIR 10
Match: AT5G25240.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 50.8 bits (120), Expect = 1.2e-06
Identity = 28/66 (42.42%), Postives = 36/66 (54.55%), Query Frame = 0

Query: 54  QVHGEGRWWTRGVRVLLKLREWSEIVAGPRWKTFIRRFNRNRSSGATRPGKFQYDPLSYA 113
           Q    G W   G   L  L+E SE +AGP+WK FIR F+  R     R   F YD  +Y+
Sbjct: 51  QEERRGNW---GSEKLKGLKEISEKIAGPKWKNFIRSFSSGRKK-MRRDVDFTYDLKNYS 110

Query: 114 MNFDEG 120
           +NFD+G
Sbjct: 111 LNFDDG 112