Moc05g33280 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g33280
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	selT-like protein
Location	chr5: 24856195 .. 24858314 (+)
RNA-Seq Expression	Moc05g33280
Synteny	Moc05g33280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCGCGCTCAGGTAGTTCTGTTAGGGTTGCCGATCTTTCTGCTTTTTACTGATATTGTGAACCTTTTCACTCCGCCGCCTCCTAAACCCGTCCATCGCCCTCCAGCTCGAGTTCACTACCAGCCTAAATCTCAAGCAGTCATCCAGGAACCGCCTGAATTTCCTACGGAGGTGAATGATTTCATGTTTGATTGAACTGTTCTTGATTTTTGTTGTTCTTTTGTGGTAATTCAGCGACTTAATTTTGTCGTCTCGTTTTGGAATGCAGAAACGGAGCGGAAACGGAGGAATTGGCTACGGCAGCATTGTTAATATCAACTTCTGTGTTTCTTGTTCTTACAGGTAGTTCCTCCTTTGATTTCATTTTGTGCGGAAAGCGAGTGAATCAAACGTTTATGTGATTTTCCTTTCCTACTTTTTTCTCATCGACTCTTATTTCGCTAGCTGAATTTGCAGTGAACTAACAGTGAAGTAAAACAGATATTTCAATTTCGCTACTCTCTAGTGGCTGTTGAGAATTATTATGACGAAAAAATGGTTTTACTCTTGAATTTTCACTATTGTTGTTTTTTTTTAAACCTCAGATTTTTTTTTTCTCTCCCTAAGCACAGGTTTCTTTTATTATCTTTAACTTCCTCGATTGAACAATCATTTCGGGGTCTCTACTCTCTAGTGGTTGATTATGATGATAAATTGGTTGTACTTTGGAATCTTCGCTGTTGTTGTTCCTTTAAACTTGATTTCTTAGTTTTTTTTAGCGCAAGTTTCTTTTATCATCGTTAACTTCCTCGATTGAACAGATATTAAAATTTTTCTACTCTGTAGCGGTTGTTGAGTGTCATTATAATTAAAAAAAAAAAAAAAAAAACTTGTTGAACTCTTGAATCTTCACTGTTGTTCCTCTATGAATGTATTTTTCTTGTTAAAGACACGAGTCTTAAACGAACACAGTTGCAGACCTCAATTCAGAAATAATTTGTAATAATTCCATTTGGAGCCAACTTTACTGTGTTAGGTTATCTGATTTGGAACAAGACAGAGAAATGGAAGTTCCAATGTTTCAATAGTTGACACCTGCTGCTTGAGATTTTAATTGAACGAACTTATTTATAACTTCAATAGATTATGTTTTGCAAGCTCAATTTCGTATAGGCTGAAAAGCAGGTTAATGTTGCATGCTCGCTTAGCTTTGGGTCTGGGATAAAACATGAACAAGAGTTATTCTTATTATGATAGAATTTACTTCATAATTTTATCGTTGATACCCAAGATAATATGGAAGAGAATTTCATTCGTGATTCCAAGTGGGCCAATAGACTATGCCAATGTTCTCCATGGAGTAAATATTGTATCTTCCCTAATTGTACAAAAGAAACATCTAACAGGCACTAATTGAAGGTGTTAGTTATTTTGTAACCATAATTGTCTTTCAGTCTGTGAACTCTGATATGTGCTCAAGAGATTTTTCTTTTCCCTACAAGAAATGATAAGAAGCTGTGTGCATGCATTTGATCCATACGTTTTGGGGTTTGCTCTCTTAAATGTAGTTGGTTCGATCATATTATCTCGGTTTGTGGAAATGCTTGATGTTAAACGGTTTTACACTTCTTGTGGCAGAGGTACTGCAATAACCATGAAAAATATGCTGGAGTCATCATTTCCTGGAGTTGAAGTTATTTTGGCAAATTACCCCCCACCACTCCCAAAGCGCCTTCTTAGCAAAGTAGTACCCGTTGTTCAATTTGGAATTATTGCAACAATAATGGCTGGCGAACAGATTTTTCCTAGGTTGGGGATGGCACCACCACCTTGGTACTACTCCTTGCGTGCAAATAGGTTTGGAAGCATTGCAAGCACTTGGCTGTTTGGAAATTTTATCCAGTCATTCTTACAAAGTTCCGGAGCCTTCGAAGTATATTGTGATGGTGAAATGGTAAGATTCTTTTGAAAGCTTATTCTGGTGAGACGACTGTTTATTGATAATATCCTTTTGTCTGAAGGTCTTCTCTAAGTTGAAGGAGCAGAGATTTCCGGGTGAGATTGAGTTGAGAGATCTTATTGGAAGGAAACTTTCCGAGTCCAGAATCATTGACAATTCTGGAGGCGTTTGGTCATAG

mRNA sequence

ATGGATCGCGCTCAGGTAGTTCTGTTAGGGTTGCCGATCTTTCTGCTTTTTACTGATATTGTGAACCTTTTCACTCCGCCGCCTCCTAAACCCGTCCATCGCCCTCCAGCTCGAGTTCACTACCAGCCTAAATCTCAAGCAGTCATCCAGGAACCGCCTGAATTTCCTACGGAGAAACGGAGCGGAAACGGAGGAATTGGCTACGGCAGCATTGTTAATATCAACTTCTGTGTTTCTTGTTCTTACAGAGGTACTGCAATAACCATGAAAAATATGCTGGAGTCATCATTTCCTGGAGTTGAAGTTATTTTGGCAAATTACCCCCCACCACTCCCAAAGCGCCTTCTTAGCAAAGTAGTACCCGTTGTTCAATTTGGAATTATTGCAACAATAATGGCTGGCGAACAGATTTTTCCTAGGTTGGGGATGGCACCACCACCTTGGTACTACTCCTTGCGTGCAAATAGGTTTGGAAGCATTGCAAGCACTTGGCTGTTTGGAAATTTTATCCAGTCATTCTTACAAAGTTCCGGAGCCTTCGAAGTATATTGTGATGGTGAAATGGTCTTCTCTAAGTTGAAGGAGCAGAGATTTCCGGGTGAGATTGAGTTGAGAGATCTTATTGGAAGGAAACTTTCCGAGTCCAGAATCATTGACAATTCTGGAGGCGTTTGGTCATAG

Coding sequence (CDS)

ATGGATCGCGCTCAGGTAGTTCTGTTAGGGTTGCCGATCTTTCTGCTTTTTACTGATATTGTGAACCTTTTCACTCCGCCGCCTCCTAAACCCGTCCATCGCCCTCCAGCTCGAGTTCACTACCAGCCTAAATCTCAAGCAGTCATCCAGGAACCGCCTGAATTTCCTACGGAGAAACGGAGCGGAAACGGAGGAATTGGCTACGGCAGCATTGTTAATATCAACTTCTGTGTTTCTTGTTCTTACAGAGGTACTGCAATAACCATGAAAAATATGCTGGAGTCATCATTTCCTGGAGTTGAAGTTATTTTGGCAAATTACCCCCCACCACTCCCAAAGCGCCTTCTTAGCAAAGTAGTACCCGTTGTTCAATTTGGAATTATTGCAACAATAATGGCTGGCGAACAGATTTTTCCTAGGTTGGGGATGGCACCACCACCTTGGTACTACTCCTTGCGTGCAAATAGGTTTGGAAGCATTGCAAGCACTTGGCTGTTTGGAAATTTTATCCAGTCATTCTTACAAAGTTCCGGAGCCTTCGAAGTATATTGTGATGGTGAAATGGTCTTCTCTAAGTTGAAGGAGCAGAGATTTCCGGGTGAGATTGAGTTGAGAGATCTTATTGGAAGGAAACTTTCCGAGTCCAGAATCATTGACAATTCTGGAGGCGTTTGGTCATAG

Protein sequence

MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKRSGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVVPVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAFEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS

Homology

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BLAST of Moc05g33280 vs. NCBI nr
Match: XP_022145727.1 (selT-like protein [Momordica charantia])

HSP 1 Score: 460.3 bits (1183), Expect = 9.6e-126
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60
           MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR
Sbjct: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60

Query: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120
           SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV
Sbjct: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120

Query: 121 PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180
           PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF
Sbjct: 121 PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180

Query: 181 EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS
Sbjct: 181 EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 226

[Close]

BLAST of Moc05g33280 vs. NCBI nr
Match: XP_038903844.1 (selT-like protein [Benincasa hispida] >XP_038903845.1 selT-like protein [Benincasa hispida] >XP_038903846.1 selT-like protein [Benincasa hispida])

HSP 1 Score: 416.8 bits (1070), Expect = 1.2e-112
Identity = 205/227 (90.31%), Postives = 213/227 (93.83%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKP-VHRPPARVHYQPKSQAVIQEPPEFPTEK 60
           MDR Q+VLLGLPIFLLFTDIVNLFTPPPPKP V+ PP RVHYQPKSQ+VIQEP EFPTEK
Sbjct: 1   MDRTQIVLLGLPIFLLFTDIVNLFTPPPPKPAVNHPPPRVHYQPKSQSVIQEPLEFPTEK 60

Query: 61  RSGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKV 120
           RS  GGIG GS++NINFCVSCSYRGTAI MKNMLESSFPGVEVILANYPPP PKRLLSKV
Sbjct: 61  RSVIGGIGQGSVININFCVSCSYRGTAIAMKNMLESSFPGVEVILANYPPPFPKRLLSKV 120

Query: 121 VPVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA 180
           VPVVQFGII TIMAGE IFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA
Sbjct: 121 VPVVQFGIIGTIMAGEHIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA 180

Query: 181 FEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           FEVYC+GEMVFSKLKEQRFPGEIELRDLIGRK+S SR +DNSGGVWS
Sbjct: 181 FEVYCNGEMVFSKLKEQRFPGEIELRDLIGRKVSGSRFVDNSGGVWS 227

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BLAST of Moc05g33280 vs. NCBI nr
Match: XP_022935564.1 (selT-like protein [Cucurbita moschata] >XP_023527743.1 selT-like protein [Cucurbita pepo subsp. pepo] >KAG6580468.1 SelT-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017218.1 SelT-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 416.8 bits (1070), Expect = 1.2e-112
Identity = 202/226 (89.38%), Postives = 211/226 (93.36%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60
           MDR Q+VLLGLPIFLLFTDIVNLFTPPPPKPV+ PP RVHYQPKSQ VIQEP EFPTEKR
Sbjct: 1   MDRTQIVLLGLPIFLLFTDIVNLFTPPPPKPVNHPPPRVHYQPKSQPVIQEPLEFPTEKR 60

Query: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120
           SG GGIG GS++NINFCVSCS+RGTAI MKNMLESSFPGVEVILANYPPPLPKRLLSKVV
Sbjct: 61  SGIGGIGQGSVININFCVSCSFRGTAINMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120

Query: 121 PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180
           PVVQFG+I  I AGE IFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF
Sbjct: 121 PVVQFGVIGIITAGEHIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180

Query: 181 EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           EVYC+GEMVFSKLKEQRFPGEIELRDLIGRKLS  R +DN+GGVWS
Sbjct: 181 EVYCNGEMVFSKLKEQRFPGEIELRDLIGRKLSGLRFVDNAGGVWS 226

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BLAST of Moc05g33280 vs. NCBI nr
Match: XP_022983567.1 (selT-like protein [Cucurbita maxima])

HSP 1 Score: 413.7 bits (1062), Expect = 1.0e-111
Identity = 201/226 (88.94%), Postives = 210/226 (92.92%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60
           MDR Q+VLLGLPIFLLFTDIVNLFTPPPPKPV+ PP RVHYQPKSQ VIQEP EFPTEKR
Sbjct: 1   MDRTQIVLLGLPIFLLFTDIVNLFTPPPPKPVNHPPPRVHYQPKSQPVIQEPLEFPTEKR 60

Query: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120
           SG G IG GS++NINFCVSCS+RGTAI MKNMLE SFPGVEVILANYPPPLPKRLLSKVV
Sbjct: 61  SGIGEIGQGSVININFCVSCSFRGTAINMKNMLELSFPGVEVILANYPPPLPKRLLSKVV 120

Query: 121 PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180
           PVVQFG+I  I AGE IFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF
Sbjct: 121 PVVQFGVIGIITAGEHIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180

Query: 181 EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           EVYC+GEMVFSKLKEQRFPGEIELRDLIGRKLS SR +DN+GGVWS
Sbjct: 181 EVYCNGEMVFSKLKEQRFPGEIELRDLIGRKLSGSRFVDNAGGVWS 226

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BLAST of Moc05g33280 vs. NCBI nr
Match: XP_004137713.1 (selT-like protein [Cucumis sativus] >KGN58749.1 hypothetical protein Csa_001631 [Cucumis sativus])

HSP 1 Score: 409.8 bits (1052), Expect = 1.5e-110
Identity = 202/227 (88.99%), Postives = 208/227 (91.63%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKP-VHRPPARVHYQPKSQAVIQEPPEFPTEK 60
           MDR Q+VLLGLPI LLFTDIVNLFTPPPPKP  + PP RVHYQPKSQ VIQEPPEFPTEK
Sbjct: 1   MDRTQMVLLGLPILLLFTDIVNLFTPPPPKPAANHPPPRVHYQPKSQPVIQEPPEFPTEK 60

Query: 61  RSGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKV 120
           RS  GGIG GS++NINFCVSCSYR TAITMKNMLESSFPGVEVILANYPPPLPKRLLSKV
Sbjct: 61  RSVIGGIGQGSVININFCVSCSYRSTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKV 120

Query: 121 VPVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA 180
           VP VQFGII  IMAGE IFPRLGM PPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA
Sbjct: 121 VPAVQFGIIGIIMAGEHIFPRLGMVPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA 180

Query: 181 FEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           FEVYC+GEMVFSKLKEQRFPGEIELRDLIGRKLS  R +DNSGGVWS
Sbjct: 181 FEVYCNGEMVFSKLKEQRFPGEIELRDLIGRKLSGLRFVDNSGGVWS 227

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BLAST of Moc05g33280 vs. ExPASy Swiss-Prot
Match: Q9STZ2 (SelT-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g47300 PE=2 SV=2)

HSP 1 Score: 280.8 bits (717), Expect = 1.4e-74
Identity = 138/215 (64.19%), Postives = 169/215 (78.60%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60
           MD+ Q++LLGLPIFLL +D+ NLFTPPPPK  H+ P            I E  +FP +K 
Sbjct: 1   MDKTQLILLGLPIFLLCSDLFNLFTPPPPKSQHQSPPS----------ISETLDFPAQKS 60

Query: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120
           +   G+GYG+ V INFC+SCSY+GTA++MK MLES FPG++V+LANYP P PKR+L+KVV
Sbjct: 61  T---GVGYGNTVEINFCISCSYKGTAVSMKKMLESVFPGLDVVLANYPAPAPKRILAKVV 120

Query: 121 PVVQFGIIATIMAGEQIFPRLGMA-PPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA 180
           PV Q G+I  IM GEQIFP +G+A PP WY+SLRANRFGS+ASTWL GNF+QSFLQSSGA
Sbjct: 121 PVAQVGVIGLIMGGEQIFPMIGIAQPPAWYHSLRANRFGSMASTWLLGNFLQSFLQSSGA 180

Query: 181 FEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSE 215
           FEV C+GE+VFSKLKE RFPGEIELRDLI   +++
Sbjct: 181 FEVSCNGELVFSKLKEGRFPGEIELRDLISGTMTK 202

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BLAST of Moc05g33280 vs. ExPASy Swiss-Prot
Match: Q9BN19 (Putative esophageal gland cell secretory protein 6 OS=Heterodera glycines OX=51029 GN=HSP6 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.7e-21
Identity = 49/145 (33.79%), Postives = 82/145 (56.55%), Query Frame = 0


Query: 72  VNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVVPVVQFGIIATI 131
           V  +FCVSC YR        +L   +PG+++   NYPP + + + ++V+ +V+  +I  +
Sbjct: 83  VKFSFCVSCGYRQAYEQFAQILREKYPGIDIHGENYPPGILRTVGAQVIGMVKIALIVCV 142

Query: 132 MAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAFEVYCDGEMVFS 191
           ++G   FP LG+  P ++  + +NR  +    +LF N I+  LQS+GAFE+Y + E ++S
Sbjct: 143 VSGRSPFPTLGLETPTFFQWMLSNRLSAALMLFLFSNAIEGMLQSTGAFEIYIESERIWS 202

Query: 192 KLKEQRFPGEIELRDLIGRKLSESR 217
           KL+  R P   EL   I   L+  R
Sbjct: 203 KLESGRVPSPPELFQAIDSHLAIRR 227

[Close]

BLAST of Moc05g33280 vs. ExPASy Swiss-Prot
Match: Q19892 (Thioredoxin reductase-like selenoprotein T homolog selt-1.2 OS=Caenorhabditis elegans OX=6239 GN=selt-1.2 PE=2 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.1e-14
Identity = 54/221 (24.43%), Postives = 96/221 (43.44%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60
           M R+  +++GL       D+      PP +      +R+     S+      P    E  
Sbjct: 1   MSRSGAIIIGLFFIASIFDVFRAEKEPPAE-----DSRLEDYLSSELETTAIPTVVNENS 60

Query: 61  SGNGGIGYG-----SIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRL 120
                +  G       + I +CVSC Y+          +  +PG+ +   N+ P   K  
Sbjct: 61  HSQDVVDSGFSKDLPKLTILYCVSCGYKQAFNQFYEFAKEKYPGLVIEGGNFSPDFWKGC 120

Query: 121 LSKVVPVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQ 180
           L+++V V + G+IA ++ G   F  +G   P    +   NRF      ++ GN  +S L 
Sbjct: 121 LAQIVGVAKIGLIAIVITGSNPFEYIGFGYPQILQTAHYNRFSYSLLVFMIGNLFESTLS 180

Query: 181 SSGAFEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESR 217
           S+GAFE++   + ++SK+ ++R P + E  +LI  +L   R
Sbjct: 181 STGAFEIFLGDKQIWSKISKERVPTQEEFLNLIDLQLKTIR 216

[Close]

BLAST of Moc05g33280 vs. ExPASy Swiss-Prot
Match: Q9U3N5 (Thioredoxin reductase-like selenoprotein T homolog selt-1.1 OS=Caenorhabditis elegans OX=6239 GN=selt-1.1 PE=2 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 1.0e-13
Identity = 39/142 (27.46%), Postives = 69/142 (48.59%), Query Frame = 0


Query: 72  VNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVVPVVQFGIIATI 131
           + I +CVSC Y+          +  +P + +  AN+ P L K  +++ +  V+  ++  +
Sbjct: 87  LRIFYCVSCGYKQAFDQFTTFAKEKYPNMPIEGANFAPVLWKAYVAQALSFVKMAVLVLV 146

Query: 132 MAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAFEVYCDGEMVFS 191
           + G   F R G+  P        N+  S    ++ GN ++  L S+GAFEVY   E ++S
Sbjct: 147 LGGINPFERFGLGYPQILQHAHGNKMSSCMLVFMLGNLVEQSLISTGAFEVYLGNEQIWS 206

Query: 192 KLKEQRFPGEIELRDLIGRKLS 214
           K++  R P   E   LI  +L+
Sbjct: 207 KIESGRVPSPQEFMQLIDAQLA 228

[Close]

BLAST of Moc05g33280 vs. ExPASy Swiss-Prot
Match: Q9VMV6 (Thioredoxin reductase-like selenoprotein T homolog CG3887 OS=Drosophila melanogaster OX=7227 GN=SelT PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.8e-13
Identity = 43/137 (31.39%), Postives = 71/137 (51.82%), Query Frame = 0


Query: 76  FCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVVPVVQFGIIATIMAGE 135
           +C SC YR        +L   +P ++V   NY PP     LSK++  ++  II ++++  
Sbjct: 48  YCYSCGYRKAFEDYVGLLGEKYPQIQVNGGNYDPPGLNYYLSKMIFALKIIIIVSVVSAV 107

Query: 136 QIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAFEVYCDGEMVFSKLKE 195
             F  LG+  P W+  ++AN+  +    +  GN +++ L SSGAFE+  +   V+SKL+ 
Sbjct: 108 SPFTFLGLNTPSWWSHMQANKIYACMMIFFLGNMLEAQLISSGAFEITLNDVPVWSKLQT 167

Query: 196 QRFPGEIELRDLIGRKL 213
            RFP    L  +I   L
Sbjct: 168 GRFPSPEVLFQIIDNHL 184

[Close]

BLAST of Moc05g33280 vs. ExPASy TrEMBL
Match: A0A6J1CXI9 (selT-like protein OS=Momordica charantia OX=3673 GN=LOC111015112 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 4.6e-126
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60
           MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR
Sbjct: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60

Query: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120
           SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV
Sbjct: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120

Query: 121 PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180
           PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF
Sbjct: 121 PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180

Query: 181 EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS
Sbjct: 181 EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 226

[Close]

BLAST of Moc05g33280 vs. ExPASy TrEMBL
Match: A0A6J1F5S6 (selT-like protein OS=Cucurbita moschata OX=3662 GN=LOC111442401 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 5.9e-113
Identity = 202/226 (89.38%), Postives = 211/226 (93.36%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60
           MDR Q+VLLGLPIFLLFTDIVNLFTPPPPKPV+ PP RVHYQPKSQ VIQEP EFPTEKR
Sbjct: 1   MDRTQIVLLGLPIFLLFTDIVNLFTPPPPKPVNHPPPRVHYQPKSQPVIQEPLEFPTEKR 60

Query: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120
           SG GGIG GS++NINFCVSCS+RGTAI MKNMLESSFPGVEVILANYPPPLPKRLLSKVV
Sbjct: 61  SGIGGIGQGSVININFCVSCSFRGTAINMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120

Query: 121 PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180
           PVVQFG+I  I AGE IFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF
Sbjct: 121 PVVQFGVIGIITAGEHIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180

Query: 181 EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           EVYC+GEMVFSKLKEQRFPGEIELRDLIGRKLS  R +DN+GGVWS
Sbjct: 181 EVYCNGEMVFSKLKEQRFPGEIELRDLIGRKLSGLRFVDNAGGVWS 226

[Close]

BLAST of Moc05g33280 vs. ExPASy TrEMBL
Match: A0A6J1IZR1 (selT-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482133 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.0e-112
Identity = 201/226 (88.94%), Postives = 210/226 (92.92%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60
           MDR Q+VLLGLPIFLLFTDIVNLFTPPPPKPV+ PP RVHYQPKSQ VIQEP EFPTEKR
Sbjct: 1   MDRTQIVLLGLPIFLLFTDIVNLFTPPPPKPVNHPPPRVHYQPKSQPVIQEPLEFPTEKR 60

Query: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120
           SG G IG GS++NINFCVSCS+RGTAI MKNMLE SFPGVEVILANYPPPLPKRLLSKVV
Sbjct: 61  SGIGEIGQGSVININFCVSCSFRGTAINMKNMLELSFPGVEVILANYPPPLPKRLLSKVV 120

Query: 121 PVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180
           PVVQFG+I  I AGE IFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF
Sbjct: 121 PVVQFGVIGIITAGEHIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGAF 180

Query: 181 EVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           EVYC+GEMVFSKLKEQRFPGEIELRDLIGRKLS SR +DN+GGVWS
Sbjct: 181 EVYCNGEMVFSKLKEQRFPGEIELRDLIGRKLSGSRFVDNAGGVWS 226

[Close]

BLAST of Moc05g33280 vs. ExPASy TrEMBL
Match: A0A0A0LCS3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G731110 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 7.2e-111
Identity = 202/227 (88.99%), Postives = 208/227 (91.63%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKP-VHRPPARVHYQPKSQAVIQEPPEFPTEK 60
           MDR Q+VLLGLPI LLFTDIVNLFTPPPPKP  + PP RVHYQPKSQ VIQEPPEFPTEK
Sbjct: 1   MDRTQMVLLGLPILLLFTDIVNLFTPPPPKPAANHPPPRVHYQPKSQPVIQEPPEFPTEK 60

Query: 61  RSGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKV 120
           RS  GGIG GS++NINFCVSCSYR TAITMKNMLESSFPGVEVILANYPPPLPKRLLSKV
Sbjct: 61  RSVIGGIGQGSVININFCVSCSYRSTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKV 120

Query: 121 VPVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA 180
           VP VQFGII  IMAGE IFPRLGM PPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA
Sbjct: 121 VPAVQFGIIGIIMAGEHIFPRLGMVPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA 180

Query: 181 FEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           FEVYC+GEMVFSKLKEQRFPGEIELRDLIGRKLS  R +DNSGGVWS
Sbjct: 181 FEVYCNGEMVFSKLKEQRFPGEIELRDLIGRKLSGLRFVDNSGGVWS 227

[Close]

BLAST of Moc05g33280 vs. ExPASy TrEMBL
Match: E5GBE6 (Selenoprotein t OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.2e-110
Identity = 200/227 (88.11%), Postives = 210/227 (92.51%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKP-VHRPPARVHYQPKSQAVIQEPPEFPTEK 60
           MDR ++VLLGLPIFLLFTDIVNLFTPPPPKP  + PP RVHYQPKSQ+VIQEP EFPTEK
Sbjct: 1   MDRTRIVLLGLPIFLLFTDIVNLFTPPPPKPAANHPPPRVHYQPKSQSVIQEPLEFPTEK 60

Query: 61  RSGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKV 120
           RS  GGIG GS++NINFCVSCSYRGTAI MKNMLESSFPGVEVILANYPPP PKRLLSKV
Sbjct: 61  RSVIGGIGQGSVININFCVSCSYRGTAINMKNMLESSFPGVEVILANYPPPFPKRLLSKV 120

Query: 121 VPVVQFGIIATIMAGEQIFPRLGMAPPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA 180
           VPVVQFGII  I+AGE IFPRLGM PPPWYYSLRANRFGSIASTWLFGNF+QSFLQSSGA
Sbjct: 121 VPVVQFGIIGIIVAGEHIFPRLGMVPPPWYYSLRANRFGSIASTWLFGNFVQSFLQSSGA 180

Query: 181 FEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNSGGVWS 227
           FEVYC+GEMVFSKLKEQRFPGEIELRDLIGRKLS SR +DNSGGVWS
Sbjct: 181 FEVYCNGEMVFSKLKEQRFPGEIELRDLIGRKLSGSRFVDNSGGVWS 227

[Close]

BLAST of Moc05g33280 vs. TAIR 10
Match: AT5G58640.1 (Selenoprotein, Rdx type )

HSP 1 Score: 302.4 bits (773), Expect = 3.1e-82
Identity = 150/226 (66.37%), Postives = 185/226 (81.86%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKP-VHRP--PARVHYQPKSQAVIQEPPEFPT 60
           MDRAQ++LLGLPIFL  +D+ NLFTPPPPKP  HRP  P  + +Q +  A+I E  +FP+
Sbjct: 1   MDRAQLILLGLPIFLFCSDLFNLFTPPPPKPQPHRPHQPPHIPHQHRPNAIIPETLDFPS 60

Query: 61  EKRSGNGG-IGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLL 120
           +K SG GG IGYG+ V INFCVSCSY+GTA+TMK MLE++FPG++VILANYPPP PKRLL
Sbjct: 61  QKTSGLGGAIGYGNTVEINFCVSCSYKGTAVTMKKMLETAFPGLDVILANYPPPAPKRLL 120

Query: 121 SKVVPVVQFGIIATIMAGEQIFPRLGMA-PPPWYYSLRANRFGSIASTWLFGNFIQSFLQ 180
           +KVVPVVQ G+I  I+AG++I P +G+  PP W++SLRANRFGS+ASTWL GNF+QS+LQ
Sbjct: 121 AKVVPVVQMGVIGMIVAGDRILPMIGITNPPAWFHSLRANRFGSMASTWLIGNFLQSYLQ 180

Query: 181 SSGAFEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNS 222
           SSGAFEV+C+GE VFSKLKE RFPGEIELRDLI + L+   I+  S
Sbjct: 181 SSGAFEVHCNGEPVFSKLKEGRFPGEIELRDLISKTLTRPSILTGS 226

[Close]

BLAST of Moc05g33280 vs. TAIR 10
Match: AT5G58640.2 (Selenoprotein, Rdx type )

HSP 1 Score: 298.1 bits (762), Expect = 5.9e-81
Identity = 146/225 (64.89%), Postives = 181/225 (80.44%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKP-VHRP--PARVHYQPKSQAVIQEPPEFPT 60
           MDRAQ++LLGLPIFL  +D+ NLFTPPPPKP  HRP  P  + +Q +  A+I E  +FP+
Sbjct: 1   MDRAQLILLGLPIFLFCSDLFNLFTPPPPKPQPHRPHQPPHIPHQHRPNAIIPETLDFPS 60

Query: 61  EKRSGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLS 120
           +     G IGYG+ V INFCVSCSY+GTA+TMK MLE++FPG++VILANYPPP PKRLL+
Sbjct: 61  QTSGLGGAIGYGNTVEINFCVSCSYKGTAVTMKKMLETAFPGLDVILANYPPPAPKRLLA 120

Query: 121 KVVPVVQFGIIATIMAGEQIFPRLGMA-PPPWYYSLRANRFGSIASTWLFGNFIQSFLQS 180
           KVVPVVQ G+I  I+AG++I P +G+  PP W++SLRANRFGS+ASTWL GNF+QS+LQS
Sbjct: 121 KVVPVVQMGVIGMIVAGDRILPMIGITNPPAWFHSLRANRFGSMASTWLIGNFLQSYLQS 180

Query: 181 SGAFEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSESRIIDNS 222
           SGAFEV+C+GE VFSKLKE RFPGEIELRDLI + L+   I+  S
Sbjct: 181 SGAFEVHCNGEPVFSKLKEGRFPGEIELRDLISKTLTRPSILTGS 225

[Close]

BLAST of Moc05g33280 vs. TAIR 10
Match: AT3G47300.1 (SELT-like protein precursor )

HSP 1 Score: 280.8 bits (717), Expect = 9.7e-76
Identity = 138/215 (64.19%), Postives = 169/215 (78.60%), Query Frame = 0


Query: 1   MDRAQVVLLGLPIFLLFTDIVNLFTPPPPKPVHRPPARVHYQPKSQAVIQEPPEFPTEKR 60
           MD+ Q++LLGLPIFLL +D+ NLFTPPPPK  H+ P            I E  +FP +K 
Sbjct: 1   MDKTQLILLGLPIFLLCSDLFNLFTPPPPKSQHQSPPS----------ISETLDFPAQKS 60

Query: 61  SGNGGIGYGSIVNINFCVSCSYRGTAITMKNMLESSFPGVEVILANYPPPLPKRLLSKVV 120
           +   G+GYG+ V INFC+SCSY+GTA++MK MLES FPG++V+LANYP P PKR+L+KVV
Sbjct: 61  T---GVGYGNTVEINFCISCSYKGTAVSMKKMLESVFPGLDVVLANYPAPAPKRILAKVV 120

Query: 121 PVVQFGIIATIMAGEQIFPRLGMA-PPPWYYSLRANRFGSIASTWLFGNFIQSFLQSSGA 180
           PV Q G+I  IM GEQIFP +G+A PP WY+SLRANRFGS+ASTWL GNF+QSFLQSSGA
Sbjct: 121 PVAQVGVIGLIMGGEQIFPMIGIAQPPAWYHSLRANRFGSMASTWLLGNFLQSFLQSSGA 180

Query: 181 FEVYCDGEMVFSKLKEQRFPGEIELRDLIGRKLSE 215
           FEV C+GE+VFSKLKE RFPGEIELRDLI   +++
Sbjct: 181 FEVSCNGELVFSKLKEGRFPGEIELRDLISGTMTK 202

The following BLAST results are available for this feature:

BLAST of Moc05g33280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145727.1	9.6e-126	100.00	selT-like protein [Momordica charantia]	[more]
XP_038903844.1	1.2e-112	90.31	selT-like protein [Benincasa hispida] >XP_038903845.1 selT-like protein [Beninca...	[more]
XP_022935564.1	1.2e-112	89.38	selT-like protein [Cucurbita moschata] >XP_023527743.1 selT-like protein [Cucurb...	[more]
XP_022983567.1	1.0e-111	88.94	selT-like protein [Cucurbita maxima]	[more]
XP_004137713.1	1.5e-110	88.99	selT-like protein [Cucumis sativus] >KGN58749.1 hypothetical protein Csa_001631 ...	[more]

BLAST of Moc05g33280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9STZ2	1.4e-74	64.19	SelT-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g47300 PE=2 SV=2	[more]
Q9BN19	8.7e-21	33.79	Putative esophageal gland cell secretory protein 6 OS=Heterodera glycines OX=510...	[more]
Q19892	2.1e-14	24.43	Thioredoxin reductase-like selenoprotein T homolog selt-1.2 OS=Caenorhabditis el...	[more]
Q9U3N5	1.0e-13	27.46	Thioredoxin reductase-like selenoprotein T homolog selt-1.1 OS=Caenorhabditis el...	[more]
Q9VMV6	1.8e-13	31.39	Thioredoxin reductase-like selenoprotein T homolog CG3887 OS=Drosophila melanoga...	[more]

BLAST of Moc05g33280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXI9	4.6e-126	100.00	selT-like protein OS=Momordica charantia OX=3673 GN=LOC111015112 PE=4 SV=1	[more]
A0A6J1F5S6	5.9e-113	89.38	selT-like protein OS=Cucurbita moschata OX=3662 GN=LOC111442401 PE=4 SV=1	[more]
A0A6J1IZR1	5.0e-112	88.94	selT-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482133 PE=4 SV=1	[more]
A0A0A0LCS3	7.2e-111	88.99	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G731110 PE=4 SV=1	[more]
E5GBE6	1.2e-110	88.11	Selenoprotein t OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]

BLAST of Moc05g33280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58640.1	3.1e-82	66.37	Selenoprotein, Rdx type	[more]
AT5G58640.2	5.9e-81	64.89	Selenoprotein, Rdx type	[more]
AT3G47300.1	9.7e-76	64.19	SELT-like protein precursor	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011893	Selenoprotein, Rdx-type	TIGRFAM	TIGR02174	TIGR02174	coord: 72..209 e-value: 2.4E-23 score: 80.2
IPR011893	Selenoprotein, Rdx-type	PFAM	PF10262	Rdx	coord: 72..208 e-value: 1.7E-15 score: 57.0
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 155..211 e-value: 1.4E-8 score: 36.5
None	No IPR available	PANTHER	PTHR13544:SF12	SELT-LIKE PROTEIN	coord: 1..221
IPR019389	Selenoprotein T	PANTHER	PTHR13544	SELENOPROTEIN T	coord: 1..221
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 70..212

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g33280.1	Moc05g33280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045454	cell redox homeostasis
biological_process	GO:0098869	cellular oxidant detoxification
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0004791	thioredoxin-disulfide reductase activity