Moc05g32020 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g32020
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Protein kinase domain-containing protein
Location	chr5: 23980386 .. 23984076 (-)
RNA-Seq Expression	Moc05g32020
Synteny	Moc05g32020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGACTCAAAGGATTATTGAATTCCCGCACAAAAATATGGATAAACGCCCAAGGAAAAGGCAGAGATTAACATGGGATATGCCTCCTCCCGTTCCTCCTCCTAAGGTATTGATTCTTGCTCTCTCGTGCAAGTACGGATTTAGAAATTTTTATTTTGTATTTTCAGACCTAGTTGTTCTAAGGATGTGGCTCTCGTAATAGTTCATTTTCCTTTTATACAATAACGAGTGCGTGTGGAATGGAGATTGAAATTCTCAAATCTTGAAATTGAAATCGACTTTTTATGGCTTTCGTCCTAATGATACTTCGGAATCAAAGCGTCCTGGTTTGACAATTCCGCACGTTTGCTTAATTTTTGACTTGTCATGAATTCAGGTTCTTCCAGCACCATACTGCGGGCAGGAATTTGGGAATGGGCAAGTTCCCAATTATGCATATCCATCTATGTATTGCAGGGGGGCACCACGGGTTGGATCTCCTCCATGGAGGCCTGATGATAAAGATGGACACTACGTATTCTCCATTGGAGAATGTTTAACCCCTCGCTGTAAGCATCTTCCAGTTCCCATTATAAGGTCATTGTCATCCCTTGTACATTTCAGACGCTTCTGGGGGAGCGTTCGGTTTTGGTCCCTGTGACAATTTCCGTTTAGGAATTTTATTTCTATTTGTTGGAATTTGTTCCTATTTTGGTAGTTAACGGATTGATCGTTGCCTCTCACAATAGATGGTGGAGGCATTTAATTATTTATTATTTTCCCCAACTTGTTTCTTGATATTTTGTCCGTATTATTGATATATTATTTCTCTTCCGTGAGCTGATATTTTTTTTTTTTTTTTGGTGCCATCTGCAGATAGAATTTTAAGCAAAATGGGTGAAGGTTAGTATTGCATCTTCAGGGACTTTTTTCCCCCCTTCTTTTCTTCTCTCTCTTTCTCTTCGGATTAGGGGATGTCAATAACATGCTTGCAATTCACTGTAAATTTGCTTTTGAGTGGAAATTCAATCCCTGTTGCATCGAATCCATTTTCTGGGTTTTATTTTTATTCATCTTTTCTTTGTCCACTTTACAAAGTTCATCGTCCATTTGTTCATCTCTGTAGGAACATTTGGGCAAGTTTTGGAATGCTTGGACAGTGAGAAAAAAGAGGTTGTAGCAATTAAAATTGTTCGCTCTATAAGTAAATATCGTGAAGCTGCCATGATCGAAATTGATGTCTTACAAAGGCTGGCCAGGCATGATATTGGAGGCACCCGGTAAGAGTTTATCCATCTTACTTCACTTTTTTGTTGAAGTTCAAAGTAAATATTAGTATAAGTGGTTTCATTCTTTGCAGTTGTGTGCAAATTCGGAATTGGTTTGACTATCGTAATCATATATGTATTGTAAGTATTTGTTAATATCTCGTGATTTGATTAATTTGGAGGGGCAATACTAGTTCCTCCATGCCTGAATATTGTGTGAGTTAAAAGAAAACTTGCGGATGAATTTCAATGGTTCTAACAGGTATTTGAGAAGCTTGGACCAAGCTTATACGATTTTCTTCGCAAAAACAGCTACCGTTCATTTCCCATTGATCTTGTTCGGGAGTTTGCCAGACAACTTCTGGAGTCTGTAGCATGTGTGTGAATAATTCTTGCAATTTTTTTACTTGATGCTTGAATAAATGTGGCATGAAGCCAAGAATTGCATATTGAAATATATCTTTTCTTATGATGACTACTGAATTGTGACACTTTTTTGTTGGAGCATGAAATATTATTCTTGTTAGATGTCTGTCTTATTGACTTTTTAAATTGTTGTCTGTAGTTATGCACGAGCTACGGCTGATTCACACTGATTTGAAGCCAGAGAATATTCTTCTTGTTTCCTCGGAGTTCATTAGAGTGCCTGACCATAAGGTATACATGGATTGCTATTGATAATCTTTGCAGTCATGCATGGGAAGGGTCATAATACTCAGTGGTTGAGATATTATGCTTGTTTTTAAAAACTGACCATTATTAGCCCTCATCCCCCCTTTTTTCCTGCATTCAAGTTTTTGTCACGTTCGGCAAAAGATGGCTCCTATTTCAAGAATCTTCCAAAGTCGGCTGCCATTAAGCTCATTGATTTTGGAAGTACCACCTCTGAACATCAAGACCACAGCTATATTGTTTCAACACGTCATTATCGGGCACCTGAAGTGATCCTGGGTACATATTTTCCCTTTTACATTATCATTGTTTATTGGTTTGCCAATGGCTATACTTTTTGCTCCCCTCGAATTACACTCTGTTGTATTGTTTGCAGGTCTAGGTTGGAATTATCCTTGCGACCTATGGAGTGTGGGATGCATACTTGTTGAACTTTGTTCTGTAAGTATCTTCTTGTAGACTAGATTAAACAATATATTGGTTTATTTATATTTTGCTTCACCCCACAGTGAAATGAAGTTGACACAACTCCAATCTCTTATGATAAAAAAAAGATTGGAAAAATATGAATTTTCTTTTTATTGTTTTCTCGTGGTTGGTGCTTTTTGGAAGAATCCTGGTAATATTTTACGTCATGAGATAATTTGTAGGACCTGACCTATAGTTCATCACGGTTTATAGTGATTAGTGGGGTATTCTGGTAATTGACTGCTACAAACTGCTGCTTTAAGAAAAGTTCAGAAAGAATGAGTTTCTTTATGTTGTCTGATTAATTTGAGGTATTTTTACAACTAGTCTGTATTTTATTTTTCCTTTTGCTTTCATAATTTTTTGCTTGCTTGCAAGCAGGGTGAGGCTCTTTTCCAAACACACGAGAACTTAGAGCATCTTGCCATGATGGAGAAGGTTTTGGGTCCATTGCCTCAACATATGATTCTGCGGGCAGAGTAAGAAATTTTGTTCTCCTTTTCTTGGGTGCTGTTCGAGTTCTTGATGAAGCTCTTATTAATTGTAATTGGTGCTTACATTTTTTCAGTCGCCGTGCTGAGAAATACTTCAGGCGGGGTGTGCAGCTCGATTGGCCCCAAGGTGCAACATCTAGAGAAAGCATGAGAGCAGTCTGGAAGTTACTGAGGCTGCCCGTAATTATTTCCACATCCATTATTTTCACTAAAATTTTAGTACTTCAAGGCATAATTCTGTGTAGTGTTCCTCTTTTGAAATGTTCTATGCAGAACCTGATAATGCAGCACGTTGATCACTCGGCTGGTGATTTAATCGATCTCCTGCAAGGCCTCTTACGCTACGATCCAGCAGAGAGGCTCAAGGCAAGGGAAGCTCTAAGACATCCTTTCTTCACTAGAGACCTAAGAAGGTGTGGTTACCCCTTGTAGTTCTCATTCACATTTTAATCAAGAGTGAGCTGCTATCAAGAGGGAGAAAGAAAGAAAGAAACTTTAAAGAAGTGATGAATTGTTTCACCAATAAGCTCATCCATCCCATCTGTTTCTTTTTTTTCCTTTTGGATTTGTCAAATTCTTGTGTAGTTAAAATCCGAACGACGATGATTTCCCCCCATTCCTTTCTCTTCCACCTGTTCATTTCTATAACATCCTATAGAATGATATGTGATCTGTTCTGGGTAAATTCCAGTTTGGCCGTGGCGGTTCTTATGCAAAATTTTACAGTATTCGTTTTTTTACATTGATGTATTCTTGCTAGTTCATTATTTCCAGGTGCTTGGGCAAAGGTTTAACCATTGGAATGTAG

mRNA sequence

ATGGAGACTCAAAGGATTATTGAATTCCCGCACAAAAATATGGATAAACGCCCAAGGAAAAGGCAGAGATTAACATGGGATATGCCTCCTCCCGTTCCTCCTCCTAAGGTTCTTCCAGCACCATACTGCGGGCAGGAATTTGGGAATGGGCAAGTTCCCAATTATGCATATCCATCTATGTATTGCAGGGGGGCACCACGGGTTGGATCTCCTCCATGGAGGCCTGATGATAAAGATGGACACTACGTATTCTCCATTGGAGAATGTTTAACCCCTCGCTATAGAATTTTAAGCAAAATGGGTGAAGGAACATTTGGGCAAGTTTTGGAATGCTTGGACAGTGAGAAAAAAGAGGTTGTAGCAATTAAAATTGTTCGCTCTATAAGTAAATATCGTGAAGCTGCCATGATCGAAATTGATGTCTTACAAAGGCTGGCCAGGCATGATATTGGAGGCACCCGTTGTGTGCAAATTCGGAATTGGTTTGACTATCGTAATCATATATGTATTGTATTTGAGAAGCTTGGACCAAGCTTATACGATTTTCTTCGCAAAAACAGCTACCGTTCATTTCCCATTGATCTTGTTCGGGAGTTTGCCAGACAACTTCTGGAGTCTGTAGCATTTATGCACGAGCTACGGCTGATTCACACTGATTTGAAGCCAGAGAATATTCTTCTTGTTTCCTCGGAGTTCATTAGAGTGCCTGACCATAAGTTTTTGTCACGTTCGGCAAAAGATGGCTCCTATTTCAAGAATCTTCCAAAGTCGGCTGCCATTAAGCTCATTGATTTTGGAAGTACCACCTCTGAACATCAAGACCACAGCTATATTGTTTCAACACGTCATTATCGGGCACCTGAAGTGATCCTGGGTCTAGGTTGGAATTATCCTTGCGACCTATGGAGTGTGGGATGCATACTTGTTGAACTTTGTTCTGGTGAGGCTCTTTTCCAAACACACGAGAACTTAGAGCATCTTGCCATGATGGAGAAGGTTTTGGGTCCATTGCCTCAACATATGATTCTGCGGGCAGATCGCCGTGCTGAGAAATACTTCAGGCGGGGTGTGCAGCTCGATTGGCCCCAAGGTGCAACATCTAGAGAAAGCATGAGAGCAGTCTGGAAGTTACTGAGGCTGCCCAACCTGATAATGCAGCACGTTGATCACTCGGCTGGTGATTTAATCGATCTCCTGCAAGGCCTCTTACGCTACGATCCAGCAGAGAGGCTCAAGGCAAGGGAAGCTCTAAGACATCCTTTCTTCACTAGAGACCTAAGAAGGTGCTTGGGCAAAGGTTTAACCATTGGAATGTAG

Coding sequence (CDS)

ATGGAGACTCAAAGGATTATTGAATTCCCGCACAAAAATATGGATAAACGCCCAAGGAAAAGGCAGAGATTAACATGGGATATGCCTCCTCCCGTTCCTCCTCCTAAGGTTCTTCCAGCACCATACTGCGGGCAGGAATTTGGGAATGGGCAAGTTCCCAATTATGCATATCCATCTATGTATTGCAGGGGGGCACCACGGGTTGGATCTCCTCCATGGAGGCCTGATGATAAAGATGGACACTACGTATTCTCCATTGGAGAATGTTTAACCCCTCGCTATAGAATTTTAAGCAAAATGGGTGAAGGAACATTTGGGCAAGTTTTGGAATGCTTGGACAGTGAGAAAAAAGAGGTTGTAGCAATTAAAATTGTTCGCTCTATAAGTAAATATCGTGAAGCTGCCATGATCGAAATTGATGTCTTACAAAGGCTGGCCAGGCATGATATTGGAGGCACCCGTTGTGTGCAAATTCGGAATTGGTTTGACTATCGTAATCATATATGTATTGTATTTGAGAAGCTTGGACCAAGCTTATACGATTTTCTTCGCAAAAACAGCTACCGTTCATTTCCCATTGATCTTGTTCGGGAGTTTGCCAGACAACTTCTGGAGTCTGTAGCATTTATGCACGAGCTACGGCTGATTCACACTGATTTGAAGCCAGAGAATATTCTTCTTGTTTCCTCGGAGTTCATTAGAGTGCCTGACCATAAGTTTTTGTCACGTTCGGCAAAAGATGGCTCCTATTTCAAGAATCTTCCAAAGTCGGCTGCCATTAAGCTCATTGATTTTGGAAGTACCACCTCTGAACATCAAGACCACAGCTATATTGTTTCAACACGTCATTATCGGGCACCTGAAGTGATCCTGGGTCTAGGTTGGAATTATCCTTGCGACCTATGGAGTGTGGGATGCATACTTGTTGAACTTTGTTCTGGTGAGGCTCTTTTCCAAACACACGAGAACTTAGAGCATCTTGCCATGATGGAGAAGGTTTTGGGTCCATTGCCTCAACATATGATTCTGCGGGCAGATCGCCGTGCTGAGAAATACTTCAGGCGGGGTGTGCAGCTCGATTGGCCCCAAGGTGCAACATCTAGAGAAAGCATGAGAGCAGTCTGGAAGTTACTGAGGCTGCCCAACCTGATAATGCAGCACGTTGATCACTCGGCTGGTGATTTAATCGATCTCCTGCAAGGCCTCTTACGCTACGATCCAGCAGAGAGGCTCAAGGCAAGGGAAGCTCTAAGACATCCTTTCTTCACTAGAGACCTAAGAAGGTGCTTGGGCAAAGGTTTAACCATTGGAATGTAG

Protein sequence

METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSMYCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVVAIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKFLSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCDLWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLDWPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHPFFTRDLRRCLGKGLTIGM

Homology

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BLAST of Moc05g32020 vs. NCBI nr
Match: XP_022145507.1 (serine/threonine-protein kinase AFC1 isoform X2 [Momordica charantia])

HSP 1 Score: 912.1 bits (2356), Expect = 1.8e-261
Identity = 438/438 (100.00%), Postives = 438/438 (100.00%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM
Sbjct: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV
Sbjct: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP
Sbjct: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420

Query: 421 FFTRDLRRCLGKGLTIGM 439
           FFTRDLRRCLGKGLTIGM
Sbjct: 421 FFTRDLRRCLGKGLTIGM 438

[Close]

BLAST of Moc05g32020 vs. NCBI nr
Match: XP_022145506.1 (serine/threonine-protein kinase AFC1 isoform X1 [Momordica charantia])

HSP 1 Score: 890.6 bits (2300), Expect = 5.6e-255
Identity = 429/433 (99.08%), Postives = 429/433 (99.08%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM
Sbjct: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV
Sbjct: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP
Sbjct: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420

Query: 421 FFTRDLRRCLGKG 434
           FFTRDLR  L  G
Sbjct: 421 FFTRDLRSSLFPG 433

[Close]

BLAST of Moc05g32020 vs. NCBI nr
Match: XP_008442583.1 (PREDICTED: serine/threonine-protein kinase AFC1 isoform X1 [Cucumis melo])

HSP 1 Score: 880.9 bits (2275), Expect = 4.4e-252
Identity = 424/443 (95.71%), Postives = 430/443 (97.07%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRIIEFPHKNMDKRPRKRQRL WDMPPPVPPPKVLP PYCGQEFGNGQVPNYAYPSM
Sbjct: 1   METQRIIEFPHKNMDKRPRKRQRLAWDMPPPVPPPKVLPPPYCGQEFGNGQVPNYAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRGAPRVGSPPWRPD+KDGHYVFSIGECLTPRY ILSKMGEGTFGQVLECLDSEKKEVV
Sbjct: 61  YCRGAPRVGSPPWRPDNKDGHYVFSIGECLTPRYTILSKMGEGTFGQVLECLDSEKKEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRS KDGSYFKNLPKSAAIKLIDFGSTT+EHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSVKDGSYFKNLPKSAAIKLIDFGSTTTEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHM+LRADRRAEKYFRRG+QLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMVLRADRRAEKYFRRGMQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQ ATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERL AREALRHP
Sbjct: 361 WPQSATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLMAREALRHP 420

Query: 421 FFTRDLRRC-----LGKGLTIGM 439
           FFTRDLR       LG+GLT+GM
Sbjct: 421 FFTRDLRSSLFPGKLGQGLTVGM 443

[Close]

BLAST of Moc05g32020 vs. NCBI nr
Match: XP_011651912.1 (serine/threonine-protein kinase AFC1 isoform X1 [Cucumis sativus])

HSP 1 Score: 879.8 bits (2272), Expect = 9.8e-252
Identity = 423/443 (95.49%), Postives = 430/443 (97.07%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRIIEFPHKNMDKRPRKRQRL WDMPPPVPPPKVLP PYCGQEFGNGQVPNYAYPSM
Sbjct: 1   METQRIIEFPHKNMDKRPRKRQRLAWDMPPPVPPPKVLPPPYCGQEFGNGQVPNYAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRGAPRVGSPPWRPD+KDGHYVFSIGECLTPRY ILSKMGEGTFGQVLECLDSEKKEVV
Sbjct: 61  YCRGAPRVGSPPWRPDNKDGHYVFSIGECLTPRYTILSKMGEGTFGQVLECLDSEKKEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRS KDGSYFKNLPKSAAIKLIDFGSTT+EHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSVKDGSYFKNLPKSAAIKLIDFGSTTTEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHM+LRADRRAEKYFRRG+QLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMVLRADRRAEKYFRRGMQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQ ATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDP+ERL AREALRHP
Sbjct: 361 WPQSATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPSERLMAREALRHP 420

Query: 421 FFTRDLRRC-----LGKGLTIGM 439
           FFTRDLR       LG+GLT+GM
Sbjct: 421 FFTRDLRSSLFPGKLGQGLTVGM 443

[Close]

BLAST of Moc05g32020 vs. NCBI nr
Match: XP_038904551.1 (serine/threonine-protein kinase AFC1 isoform X2 [Benincasa hispida])

HSP 1 Score: 879.0 bits (2270), Expect = 1.7e-251
Identity = 419/433 (96.77%), Postives = 424/433 (97.92%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRI EFPHKNMDKRPRKRQRL WDMPPP PPPKVLP PYCGQEFGNGQVPN+AYPSM
Sbjct: 1   METQRITEFPHKNMDKRPRKRQRLAWDMPPPAPPPKVLPPPYCGQEFGNGQVPNFAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRG PRVGSPPWRPD+KDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEK EVV
Sbjct: 61  YCRGGPRVGSPPWRPDNKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKNEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRS KDGSYFKNLPKSAAIKLIDFGSTT+EHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSVKDGSYFKNLPKSAAIKLIDFGSTTTEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHM+LRADRRAEKYFRRG+QLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMVLRADRRAEKYFRRGMQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERL AREALRHP
Sbjct: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLMAREALRHP 420

Query: 421 FFTRDLRRCLGKG 434
           FFTRDLRRCL KG
Sbjct: 421 FFTRDLRRCLDKG 433

[Close]

BLAST of Moc05g32020 vs. ExPASy Swiss-Prot
Match: P51566 (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=2 SV=2)

HSP 1 Score: 753.4 bits (1944), Expect = 1.4e-216
Identity = 352/429 (82.05%), Postives = 389/429 (90.68%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWD-----MPPPVPPPKVLPAPYCGQEFGNGQVPNY 60
           +ETQR +EFPH+ +DKRPRKR RLTWD     +PPP PP    P  Y G EF +G VPN+
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  AYPSMYCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSE 120
            YP+M+  G PR GSPPWRPDDKDGHYVF +G+ LTPRY+ILSKMGEGTFGQVLEC D++
Sbjct: 77  VYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNK 136

Query: 121 KKEVVAIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKL 180
            KEVVAIK++RSI+KYREAAMIEIDVLQRL RHD+GG+RCVQIRNWFDYRNHICIVFEKL
Sbjct: 137 NKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKL 196

Query: 181 GPSLYDFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRV 240
           GPSLYDFLRKNSYRSFPIDLVRE  RQLLESVA+MH+LRLIHTDLKPENILLVSSE+I++
Sbjct: 197 GPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKI 256

Query: 241 PDHKFLSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGW 300
           PD+KFLSR  KDGSYFKNLPKS+AIKLIDFGSTT EHQDH+YIVSTRHYRAPEVILG+GW
Sbjct: 257 PDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGW 316

Query: 301 NYPCDLWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRR 360
           NYPCDLWS+GCILVELCSGEALFQTHENLEHLAMME+VLGPLP HM+LRADRR+EKYFRR
Sbjct: 317 NYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRR 376

Query: 361 GVQLDWPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKARE 420
           G +LDWP+GATSR+S++AVWKL RLPNLIMQHVDHSAGDLIDLLQGLLRYDP ER KARE
Sbjct: 377 GAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKARE 436

Query: 421 ALRHPFFTR 425
           AL HPFFTR
Sbjct: 437 ALNHPFFTR 445

[Close]

BLAST of Moc05g32020 vs. ExPASy Swiss-Prot
Match: P51567 (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=1 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 1.1e-176
Identity = 300/430 (69.77%), Postives = 356/430 (82.79%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGN------GQVPN 60
           ME +R+ EFPH +MD+RPRKR RL WD+ P     +V    +CGQE GN         P+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQV--GMFCGQEIGNISSFASSGAPS 60

Query: 61  YAYPSMYCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDS 120
               S+  +G  R GSPPWR DDKDGHY+F +G+ LTPRY+I SKMGEGTFGQVLEC D 
Sbjct: 61  DNSSSLCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDR 120

Query: 121 EKKEVVAIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEK 180
           E+KE+VA+KIVR + KYREAAMIEI++LQ+L +HD GG RCVQIRNWFDYRNHICIVFEK
Sbjct: 121 ERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEK 180

Query: 181 LGPSLYDFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIR 240
           LG SLYDFLRKN+YRSFPIDLVRE   QLLE VAFMH+LR+IHTDLKPENILLVSS++++
Sbjct: 181 LGSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVK 240

Query: 241 VPDHKFLSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLG 300
           +P++K  SR  +D  Y K +PKS+AIK+IDFGSTT E QD +YIVSTRHYRAPEVILGLG
Sbjct: 241 IPEYKG-SRLQRDVCY-KRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLG 300

Query: 301 WNYPCDLWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFR 360
           W+YPCD+WSVGCI+VELC+GEALFQTHENLEHLAMME+VLGP PQ M+ + DR +EKY R
Sbjct: 301 WSYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVR 360

Query: 361 RGVQLDWPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAR 420
           RG +LDWP GATSR+S++AV KL RL NLIMQHVDHSAG+LI+++QGLLR+DP+ER+ AR
Sbjct: 361 RG-RLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAR 420

Query: 421 EALRHPFFTR 425
           EALRHPFF R
Sbjct: 421 EALRHPFFAR 425

[Close]

BLAST of Moc05g32020 vs. ExPASy Swiss-Prot
Match: P51568 (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=1 SV=2)

HSP 1 Score: 528.9 bits (1361), Expect = 5.6e-149
Identity = 262/412 (63.59%), Postives = 320/412 (77.67%), Query Frame = 0


Query: 12  KNMDK-RPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSMYCRGAPRVGS 71
           ++MDK R RKR R+TWD  P  P  K       G +                    R+ S
Sbjct: 7   ESMDKERVRKRPRMTWDEAPAEPEAKRAVIKGHGSD-------------------GRILS 66

Query: 72  PPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVVAIKIVRSISK 131
           PP R DD+DGHYVFS+ + LTPRY+ILSKMGEGTFG+VLEC D + KE VAIKI+RSI K
Sbjct: 67  PPLRDDDRDGHYVFSLRDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKK 126

Query: 132 YREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRS 191
           YR+AAMIEIDVLQ+L + D G TRCVQ++NWFDYRNHICIVFEKLGPSL+DFL++N Y +
Sbjct: 127 YRDAAMIEIDVLQKLVKSDKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSA 186

Query: 192 FPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKFLSRSAKDGSY 251
           FP+ LVR+F  QLLESVA+MHEL+L+HTDLKPENILLVSSE +++PD+K   RSA + ++
Sbjct: 187 FPLALVRDFGCQLLESVAYMHELQLVHTDLKPENILLVSSENVKLPDNK---RSAANETH 246

Query: 252 FKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCDLWSVGCILVE 311
           F+ LPKS+AIKLIDFGST  +++ H  IV TRHYR+PEVILGLGW+Y CDLWS+GCIL E
Sbjct: 247 FRCLPKSSAIKLIDFGSTVCDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFE 306

Query: 312 LCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLDWPQGATSRES 371
           LC+GEALFQTH+NLEHLAMME+ LGPLP+HM  +A R AEKYFRRG +L+WP+GA SRES
Sbjct: 307 LCTGEALFQTHDNLEHLAMMERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRES 366

Query: 372 MRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHPFF 423
           +RAV +L RL +++ +HVD++     DLL GLL YDP+ERL A EAL HPFF
Sbjct: 367 IRAVKRLDRLKDMVSKHVDNTRSRFADLLYGLLAYDPSERLTANEALDHPFF 396

[Close]

BLAST of Moc05g32020 vs. ExPASy Swiss-Prot
Match: Q10156 (Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=lkh1 PE=1 SV=3)

HSP 1 Score: 336.7 bits (862), Expect = 4.1e-91
Identity = 167/354 (47.18%), Postives = 236/354 (66.67%), Query Frame = 0


Query: 76  DDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVVAIKIVRSISKYREAA 135
           DD DGHY          RY ++  +G GTFG+V++C D       AIK+ R+I KYREA+
Sbjct: 344 DDDDGHYKVVPNSKFANRYTVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTRAIPKYREAS 403

Query: 136 MIEIDVLQRLARHD-IGGTRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPID 195
           +IE+ VLQ +A  D     +C+Q+R++FDYR HICIV +  G S++DFL+ N+Y  FP+ 
Sbjct: 404 LIELRVLQTIAHSDPTNENKCIQLRDYFDYRKHICIVTDLFGWSVFDFLKNNNYIPFPLK 463

Query: 196 LVREFARQLLESVAFMHELRLIHTDLKPENILLVS--SEFIRVPDHKFLSRSAKDGSYFK 255
            ++  ++QL +SVAF+H L L+HTDLKPEN+LLVS  S  IR+P            +Y +
Sbjct: 464 HIQMLSQQLFKSVAFLHSLGLVHTDLKPENVLLVSNASRTIRLPYR----------NYSQ 523

Query: 256 NLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCDLWSVGCILVELC 315
            +  S  I+LIDFGS T E + HS +VSTRHYRAPE+ILGLGW+YPCD+WS+GCILVEL 
Sbjct: 524 KVLNSCEIRLIDFGSATFEDEYHSSVVSTRHYRAPEIILGLGWSYPCDVWSIGCILVELF 583

Query: 316 SGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLDWPQGATSRESMR 375
           +G+ALFQTHE+ EHL MMEK+LGP  ++MI R+ R ++++F+   ++ +P   T ++S+ 
Sbjct: 584 TGQALFQTHEDSEHLCMMEKILGPFDRNMISRSSRTSQRFFKSDGKVRYPLSNTPKKSIN 643

Query: 376 AVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHPFFTRDL 427
            +  L  L  +        A  L+DLL+ +  YDP  R+ A+EAL HPFFT+ +
Sbjct: 644 YLQSLQTLEQIFAVSSPEVA-LLLDLLKKVFVYDPKRRITAKEALWHPFFTQPI 686

[Close]

BLAST of Moc05g32020 vs. ExPASy Swiss-Prot
Match: P49760 (Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.7e-89
Identity = 166/351 (47.29%), Postives = 237/351 (67.52%), Query Frame = 0


Query: 76  DDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEV-VAIKIVRSISKYREA 135
           DD +GH ++ +G+ L  RY I+S +GEGTFG+V++C+D  +    VA+KI++++ KY+EA
Sbjct: 145 DDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEA 204

Query: 136 AMIEIDVLQRLARHDIGGTR-CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPI 195
           A +EI+VL+++   D      CVQ+ +WFDY  H+CI FE LG S +DFL+ N+Y  +PI
Sbjct: 205 ARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPI 264

Query: 196 DLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKFLSRSAKDGSYFKN 255
             VR  A QL ++V F+H+ +L HTDLKPENIL V+S++    +  +     +D    + 
Sbjct: 265 HQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDY----ELTYNLEKKRD----ER 324

Query: 256 LPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCDLWSVGCILVELCS 315
             KS A++++DFGS T +H+ HS IVSTRHYRAPEVIL LGW+ PCD+WS+GCI+ E   
Sbjct: 325 SVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYV 384

Query: 316 GEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLDWPQGATSRESMRA 375
           G  LFQTH+N EHLAMME++LGP+P  MI +   R +KYF RG +LDW +  ++   +R 
Sbjct: 385 GFTLFQTHDNREHLAMMERILGPIPSRMIRKT--RKQKYFYRG-RLDWDENTSAGRYVRE 444

Query: 376 VWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHPFFTR 425
             K LR   L  +  +H    L DL++ +L Y+PA+RL   EAL+HPFF R
Sbjct: 445 NCKPLR-RYLTSEAEEHH--QLFDLIESMLEYEPAKRLTLGEALQHPFFAR 481

[Close]

BLAST of Moc05g32020 vs. ExPASy TrEMBL
Match: A0A6J1CWT0 (serine/threonine-protein kinase AFC1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014941 PE=4 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 8.7e-262
Identity = 438/438 (100.00%), Postives = 438/438 (100.00%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM
Sbjct: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV
Sbjct: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP
Sbjct: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420

Query: 421 FFTRDLRRCLGKGLTIGM 439
           FFTRDLRRCLGKGLTIGM
Sbjct: 421 FFTRDLRRCLGKGLTIGM 438

[Close]

BLAST of Moc05g32020 vs. ExPASy TrEMBL
Match: A0A6J1CWI1 (serine/threonine-protein kinase AFC1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014941 PE=4 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 2.7e-255
Identity = 429/433 (99.08%), Postives = 429/433 (99.08%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM
Sbjct: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV
Sbjct: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP
Sbjct: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420

Query: 421 FFTRDLRRCLGKG 434
           FFTRDLR  L  G
Sbjct: 421 FFTRDLRSSLFPG 433

[Close]

BLAST of Moc05g32020 vs. ExPASy TrEMBL
Match: A0A1S3B5J2 (serine/threonine-protein kinase AFC1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486413 PE=4 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.1e-252
Identity = 424/443 (95.71%), Postives = 430/443 (97.07%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRIIEFPHKNMDKRPRKRQRL WDMPPPVPPPKVLP PYCGQEFGNGQVPNYAYPSM
Sbjct: 1   METQRIIEFPHKNMDKRPRKRQRLAWDMPPPVPPPKVLPPPYCGQEFGNGQVPNYAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRGAPRVGSPPWRPD+KDGHYVFSIGECLTPRY ILSKMGEGTFGQVLECLDSEKKEVV
Sbjct: 61  YCRGAPRVGSPPWRPDNKDGHYVFSIGECLTPRYTILSKMGEGTFGQVLECLDSEKKEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRS KDGSYFKNLPKSAAIKLIDFGSTT+EHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSVKDGSYFKNLPKSAAIKLIDFGSTTTEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHM+LRADRRAEKYFRRG+QLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMVLRADRRAEKYFRRGMQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQ ATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERL AREALRHP
Sbjct: 361 WPQSATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLMAREALRHP 420

Query: 421 FFTRDLRRC-----LGKGLTIGM 439
           FFTRDLR       LG+GLT+GM
Sbjct: 421 FFTRDLRSSLFPGKLGQGLTVGM 443

[Close]

BLAST of Moc05g32020 vs. ExPASy TrEMBL
Match: A0A1S3B614 (serine/threonine-protein kinase AFC1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486413 PE=4 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.1e-251
Identity = 419/429 (97.67%), Postives = 423/429 (98.60%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRIIEFPHKNMDKRPRKRQRL WDMPPPVPPPKVLP PYCGQEFGNGQVPNYAYPSM
Sbjct: 1   METQRIIEFPHKNMDKRPRKRQRLAWDMPPPVPPPKVLPPPYCGQEFGNGQVPNYAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRGAPRVGSPPWRPD+KDGHYVFSIGECLTPRY ILSKMGEGTFGQVLECLDSEKKEVV
Sbjct: 61  YCRGAPRVGSPPWRPDNKDGHYVFSIGECLTPRYTILSKMGEGTFGQVLECLDSEKKEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRS KDGSYFKNLPKSAAIKLIDFGSTT+EHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSVKDGSYFKNLPKSAAIKLIDFGSTTTEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHM+LRADRRAEKYFRRG+QLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMVLRADRRAEKYFRRGMQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQ ATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERL AREALRHP
Sbjct: 361 WPQSATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLMAREALRHP 420

Query: 421 FFTRDLRRC 430
           FFTRDLRRC
Sbjct: 421 FFTRDLRRC 429

[Close]

BLAST of Moc05g32020 vs. ExPASy TrEMBL
Match: A0A0A0LDS4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734970 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 6.9e-251
Identity = 418/429 (97.44%), Postives = 423/429 (98.60%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGNGQVPNYAYPSM 60
           METQRIIEFPHKNMDKRPRKRQRL WDMPPPVPPPKVLP PYCGQEFGNGQVPNYAYPSM
Sbjct: 1   METQRIIEFPHKNMDKRPRKRQRLAWDMPPPVPPPKVLPPPYCGQEFGNGQVPNYAYPSM 60

Query: 61  YCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSEKKEVV 120
           YCRGAPRVGSPPWRPD+KDGHYVFSIGECLTPRY ILSKMGEGTFGQVLECLDSEKKEVV
Sbjct: 61  YCRGAPRVGSPPWRPDNKDGHYVFSIGECLTPRYTILSKMGEGTFGQVLECLDSEKKEVV 120

Query: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180
           AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY
Sbjct: 121 AIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKLGPSLY 180

Query: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240
           DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF
Sbjct: 181 DFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRVPDHKF 240

Query: 241 LSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300
           LSRS KDGSYFKNLPKSAAIKLIDFGSTT+EHQDHSYIVSTRHYRAPEVILGLGWNYPCD
Sbjct: 241 LSRSVKDGSYFKNLPKSAAIKLIDFGSTTTEHQDHSYIVSTRHYRAPEVILGLGWNYPCD 300

Query: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRRGVQLD 360
           LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHM+LRADRRAEKYFRRG+QLD
Sbjct: 301 LWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMVLRADRRAEKYFRRGMQLD 360

Query: 361 WPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAREALRHP 420
           WPQ ATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDP+ERL AREALRHP
Sbjct: 361 WPQSATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPSERLMAREALRHP 420

Query: 421 FFTRDLRRC 430
           FFTRDLRRC
Sbjct: 421 FFTRDLRRC 429

[Close]

BLAST of Moc05g32020 vs. TAIR 10
Match: AT3G53570.3 (FUS3-complementing gene 1 )

HSP 1 Score: 753.4 bits (1944), Expect = 9.9e-218
Identity = 352/429 (82.05%), Postives = 389/429 (90.68%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWD-----MPPPVPPPKVLPAPYCGQEFGNGQVPNY 60
           +ETQR +EFPH+ +DKRPRKR RLTWD     +PPP PP    P  Y G EF +G VPN+
Sbjct: 3   LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 62

Query: 61  AYPSMYCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSE 120
            YP+M+  G PR GSPPWRPDDKDGHYVF +G+ LTPRY+ILSKMGEGTFGQVLEC D++
Sbjct: 63  VYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNK 122

Query: 121 KKEVVAIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKL 180
            KEVVAIK++RSI+KYREAAMIEIDVLQRL RHD+GG+RCVQIRNWFDYRNHICIVFEKL
Sbjct: 123 NKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKL 182

Query: 181 GPSLYDFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRV 240
           GPSLYDFLRKNSYRSFPIDLVRE  RQLLESVA+MH+LRLIHTDLKPENILLVSSE+I++
Sbjct: 183 GPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKI 242

Query: 241 PDHKFLSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGW 300
           PD+KFLSR  KDGSYFKNLPKS+AIKLIDFGSTT EHQDH+YIVSTRHYRAPEVILG+GW
Sbjct: 243 PDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGW 302

Query: 301 NYPCDLWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRR 360
           NYPCDLWS+GCILVELCSGEALFQTHENLEHLAMME+VLGPLP HM+LRADRR+EKYFRR
Sbjct: 303 NYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRR 362

Query: 361 GVQLDWPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKARE 420
           G +LDWP+GATSR+S++AVWKL RLPNLIMQHVDHSAGDLIDLLQGLLRYDP ER KARE
Sbjct: 363 GAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKARE 422

Query: 421 ALRHPFFTR 425
           AL HPFFTR
Sbjct: 423 ALNHPFFTR 431

[Close]

BLAST of Moc05g32020 vs. TAIR 10
Match: AT3G53570.1 (FUS3-complementing gene 1 )

HSP 1 Score: 753.4 bits (1944), Expect = 9.9e-218
Identity = 352/429 (82.05%), Postives = 389/429 (90.68%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWD-----MPPPVPPPKVLPAPYCGQEFGNGQVPNY 60
           +ETQR +EFPH+ +DKRPRKR RLTWD     +PPP PP    P  Y G EF +G VPN+
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  AYPSMYCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSE 120
            YP+M+  G PR GSPPWRPDDKDGHYVF +G+ LTPRY+ILSKMGEGTFGQVLEC D++
Sbjct: 77  VYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNK 136

Query: 121 KKEVVAIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKL 180
            KEVVAIK++RSI+KYREAAMIEIDVLQRL RHD+GG+RCVQIRNWFDYRNHICIVFEKL
Sbjct: 137 NKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKL 196

Query: 181 GPSLYDFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRV 240
           GPSLYDFLRKNSYRSFPIDLVRE  RQLLESVA+MH+LRLIHTDLKPENILLVSSE+I++
Sbjct: 197 GPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKI 256

Query: 241 PDHKFLSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGW 300
           PD+KFLSR  KDGSYFKNLPKS+AIKLIDFGSTT EHQDH+YIVSTRHYRAPEVILG+GW
Sbjct: 257 PDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGW 316

Query: 301 NYPCDLWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRR 360
           NYPCDLWS+GCILVELCSGEALFQTHENLEHLAMME+VLGPLP HM+LRADRR+EKYFRR
Sbjct: 317 NYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRR 376

Query: 361 GVQLDWPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKARE 420
           G +LDWP+GATSR+S++AVWKL RLPNLIMQHVDHSAGDLIDLLQGLLRYDP ER KARE
Sbjct: 377 GAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKARE 436

Query: 421 ALRHPFFTR 425
           AL HPFFTR
Sbjct: 437 ALNHPFFTR 445

[Close]

BLAST of Moc05g32020 vs. TAIR 10
Match: AT3G53570.2 (FUS3-complementing gene 1 )

HSP 1 Score: 753.4 bits (1944), Expect = 9.9e-218
Identity = 352/429 (82.05%), Postives = 389/429 (90.68%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWD-----MPPPVPPPKVLPAPYCGQEFGNGQVPNY 60
           +ETQR +EFPH+ +DKRPRKR RLTWD     +PPP PP    P  Y G EF +G VPN+
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  AYPSMYCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSE 120
            YP+M+  G PR GSPPWRPDDKDGHYVF +G+ LTPRY+ILSKMGEGTFGQVLEC D++
Sbjct: 77  VYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNK 136

Query: 121 KKEVVAIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKL 180
            KEVVAIK++RSI+KYREAAMIEIDVLQRL RHD+GG+RCVQIRNWFDYRNHICIVFEKL
Sbjct: 137 NKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKL 196

Query: 181 GPSLYDFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRV 240
           GPSLYDFLRKNSYRSFPIDLVRE  RQLLESVA+MH+LRLIHTDLKPENILLVSSE+I++
Sbjct: 197 GPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKI 256

Query: 241 PDHKFLSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGW 300
           PD+KFLSR  KDGSYFKNLPKS+AIKLIDFGSTT EHQDH+YIVSTRHYRAPEVILG+GW
Sbjct: 257 PDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGW 316

Query: 301 NYPCDLWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRR 360
           NYPCDLWS+GCILVELCSGEALFQTHENLEHLAMME+VLGPLP HM+LRADRR+EKYFRR
Sbjct: 317 NYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRR 376

Query: 361 GVQLDWPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKARE 420
           G +LDWP+GATSR+S++AVWKL RLPNLIMQHVDHSAGDLIDLLQGLLRYDP ER KARE
Sbjct: 377 GAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKARE 436

Query: 421 ALRHPFFTR 425
           AL HPFFTR
Sbjct: 437 ALNHPFFTR 445

[Close]

BLAST of Moc05g32020 vs. TAIR 10
Match: AT3G53570.4 (FUS3-complementing gene 1 )

HSP 1 Score: 753.4 bits (1944), Expect = 9.9e-218
Identity = 352/429 (82.05%), Postives = 389/429 (90.68%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWD-----MPPPVPPPKVLPAPYCGQEFGNGQVPNY 60
           +ETQR +EFPH+ +DKRPRKR RLTWD     +PPP PP    P  Y G EF +G VPN+
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  AYPSMYCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDSE 120
            YP+M+  G PR GSPPWRPDDKDGHYVF +G+ LTPRY+ILSKMGEGTFGQVLEC D++
Sbjct: 77  VYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNK 136

Query: 121 KKEVVAIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEKL 180
            KEVVAIK++RSI+KYREAAMIEIDVLQRL RHD+GG+RCVQIRNWFDYRNHICIVFEKL
Sbjct: 137 NKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKL 196

Query: 181 GPSLYDFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIRV 240
           GPSLYDFLRKNSYRSFPIDLVRE  RQLLESVA+MH+LRLIHTDLKPENILLVSSE+I++
Sbjct: 197 GPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKI 256

Query: 241 PDHKFLSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLGW 300
           PD+KFLSR  KDGSYFKNLPKS+AIKLIDFGSTT EHQDH+YIVSTRHYRAPEVILG+GW
Sbjct: 257 PDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGW 316

Query: 301 NYPCDLWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFRR 360
           NYPCDLWS+GCILVELCSGEALFQTHENLEHLAMME+VLGPLP HM+LRADRR+EKYFRR
Sbjct: 317 NYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRR 376

Query: 361 GVQLDWPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKARE 420
           G +LDWP+GATSR+S++AVWKL RLPNLIMQHVDHSAGDLIDLLQGLLRYDP ER KARE
Sbjct: 377 GAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKARE 436

Query: 421 ALRHPFFTR 425
           AL HPFFTR
Sbjct: 437 ALNHPFFTR 445

[Close]

BLAST of Moc05g32020 vs. TAIR 10
Match: AT4G24740.1 (FUS3-complementing gene 2 )

HSP 1 Score: 620.9 bits (1600), Expect = 7.7e-178
Identity = 300/430 (69.77%), Postives = 356/430 (82.79%), Query Frame = 0


Query: 1   METQRIIEFPHKNMDKRPRKRQRLTWDMPPPVPPPKVLPAPYCGQEFGN------GQVPN 60
           ME +R+ EFPH +MD+RPRKR RL WD+ P     +V    +CGQE GN         P+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQV--GMFCGQEIGNISSFASSGAPS 60

Query: 61  YAYPSMYCRGAPRVGSPPWRPDDKDGHYVFSIGECLTPRYRILSKMGEGTFGQVLECLDS 120
               S+  +G  R GSPPWR DDKDGHY+F +G+ LTPRY+I SKMGEGTFGQVLEC D 
Sbjct: 61  DNSSSLCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDR 120

Query: 121 EKKEVVAIKIVRSISKYREAAMIEIDVLQRLARHDIGGTRCVQIRNWFDYRNHICIVFEK 180
           E+KE+VA+KIVR + KYREAAMIEI++LQ+L +HD GG RCVQIRNWFDYRNHICIVFEK
Sbjct: 121 ERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEK 180

Query: 181 LGPSLYDFLRKNSYRSFPIDLVREFARQLLESVAFMHELRLIHTDLKPENILLVSSEFIR 240
           LG SLYDFLRKN+YRSFPIDLVRE   QLLE VAFMH+LR+IHTDLKPENILLVSS++++
Sbjct: 181 LGSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVK 240

Query: 241 VPDHKFLSRSAKDGSYFKNLPKSAAIKLIDFGSTTSEHQDHSYIVSTRHYRAPEVILGLG 300
           +P++K  SR  +D  Y K +PKS+AIK+IDFGSTT E QD +YIVSTRHYRAPEVILGLG
Sbjct: 241 IPEYKG-SRLQRDVCY-KRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLG 300

Query: 301 WNYPCDLWSVGCILVELCSGEALFQTHENLEHLAMMEKVLGPLPQHMILRADRRAEKYFR 360
           W+YPCD+WSVGCI+VELC+GEALFQTHENLEHLAMME+VLGP PQ M+ + DR +EKY R
Sbjct: 301 WSYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVR 360

Query: 361 RGVQLDWPQGATSRESMRAVWKLLRLPNLIMQHVDHSAGDLIDLLQGLLRYDPAERLKAR 420
           RG +LDWP GATSR+S++AV KL RL NLIMQHVDHSAG+LI+++QGLLR+DP+ER+ AR
Sbjct: 361 RG-RLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAR 420

Query: 421 EALRHPFFTR 425
           EALRHPFF R
Sbjct: 421 EALRHPFFAR 425

The following BLAST results are available for this feature:

BLAST of Moc05g32020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145507.1	1.8e-261	100.00	serine/threonine-protein kinase AFC1 isoform X2 [Momordica charantia]	[more]
XP_022145506.1	5.6e-255	99.08	serine/threonine-protein kinase AFC1 isoform X1 [Momordica charantia]	[more]
XP_008442583.1	4.4e-252	95.71	PREDICTED: serine/threonine-protein kinase AFC1 isoform X1 [Cucumis melo]	[more]
XP_011651912.1	9.8e-252	95.49	serine/threonine-protein kinase AFC1 isoform X1 [Cucumis sativus]	[more]
XP_038904551.1	1.7e-251	96.77	serine/threonine-protein kinase AFC1 isoform X2 [Benincasa hispida]	[more]

BLAST of Moc05g32020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51566	1.4e-216	82.05	Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=...	[more]
P51567	1.1e-176	69.77	Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=...	[more]
P51568	5.6e-149	63.59	Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=...	[more]
Q10156	4.1e-91	47.18	Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe (strain 972 / ...	[more]
P49760	1.7e-89	47.29	Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1	[more]

BLAST of Moc05g32020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CWT0	8.7e-262	100.00	serine/threonine-protein kinase AFC1 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1CWI1	2.7e-255	99.08	serine/threonine-protein kinase AFC1 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A1S3B5J2	2.1e-252	95.71	serine/threonine-protein kinase AFC1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S3B614	3.1e-251	97.67	serine/threonine-protein kinase AFC1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0LDS4	6.9e-251	97.44	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734...	[more]

BLAST of Moc05g32020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53570.3	9.9e-218	82.05	FUS3-complementing gene 1	[more]
AT3G53570.1	9.9e-218	82.05	FUS3-complementing gene 1	[more]
AT3G53570.2	9.9e-218	82.05	FUS3-complementing gene 1	[more]
AT3G53570.4	9.9e-218	82.05	FUS3-complementing gene 1	[more]
AT4G24740.1	7.7e-178	69.77	FUS3-complementing gene 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 94..422 e-value: 6.7E-74 score: 261.5
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 94..422 e-value: 1.1E-49 score: 169.2
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 94..422 score: 39.014801
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 81..174 e-value: 3.2E-112 score: 377.1
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 148..422 e-value: 3.2E-112 score: 377.1
None	No IPR available	PIRSR	PIRSR000606-51	PIRSR000606-51	coord: 91..333 e-value: 1.4E-24 score: 84.3
None	No IPR available	PIRSR	PIRSR630220-2	PIRSR630220-2	coord: 89..241 e-value: 5.7E-6 score: 22.5
None	No IPR available	PANTHER	PTHR45646	SERINE/THREONINE-PROTEIN KINASE DOA-RELATED	coord: 1..425
None	No IPR available	PANTHER	PTHR45646:SF17	BNAA07G37640D PROTEIN	coord: 1..425
None	No IPR available	CDD	cd14134	PKc_CLK	coord: 81..422 e-value: 0.0 score: 553.712
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 215..227
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 81..427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g32020.1	Moc05g32020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity