Moc05g29840 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g29840
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr5: 22197577 .. 22199827 (-)
RNA-Seq ExpressionMoc05g29840
SyntenyMoc05g29840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAGCACTGAGCTTAAGAGGTAAATTACACCCACACAACTATTTGGCTTTCCTTCTGAATTTTTCTTCTCTTTAGTTCCTCTATCTCCAAAAAAAAAATATATATATTTTTTTTCTATATTCTTTTTTAGGTATTTACATAAATAGCATAAAATAAATTTAATATGTAAGTTGCCAGAACTTCTCAATTTTTAAAAATATAGCAGAAGTGTGAATTTTCTAATTAGTGCGTTTTATATTATGTTTATTTGATAATACCAATTTATTTTATGCTATTTAGGTAAACGTTTTGAATATTTAAATAGTTTTACTTATATTGATATTTTGGGATGTGTGTGAGAATAAATGATCTTTTTATATCATTGAAGAAGTATACTAATAATATGCTTATTCTTGTATCATTGATGACGTCAGCGGTCGGAAAATTTTCTTTTTTTATCTCTCTATACAAACCGACCGTTTAACCGACTGTTCGAACGGTCGGTTTGACCGGTTTTTAAGAGTAGAGAACTGTACTTGTGTGTCTGAAATGCAAGGAAGATCGAAAGAAGGAAGAAAGAGAAAAAATGGTAGAGAGAGAAACGAAAAAGAGAAGAAGGAATGAGATAAAAAGGAAGGACTACTCAAGCGCGACGTAGGTTAGGTGGGATAGAAAAGATGGTAAAAAAGTATAGTATAATAATAAATAAATATTATATATAACTCACGTGAATCACGTGAAATTGATTTTTTTCTCTTTTATATTTTTCGACCCACGTGGCTTTCTATTTTTTTTCTCCTCCTCTTTACTCTTACATCTTTCTTTTTTTTTCACTCTCAGTTGTCATTTATCCTCTCGTTTCTCTCTGTGCAGTTCTCTCTCTCGAGGAAGAAATTTTAGATTTTTTTTCTAAATAAATCGATTCCTCATTCGATTTCTTTAATATTGGAAGAGCCGACCGACCAACTAGTCCATGTTTTATAGAGAAAAACTGACATCGACCGACATCGGTTCGATAGATTTTAGTCGGTCGGATCGATTTTTTGACTTGGTTTCATCAGCCCTAATACCTTGTATAAAATACATCACCAATATACATGCCTGTGCCATTTTTATAAGATATTTTATTTTGTGATGACATTTTTCATCCAAATAATTAGATGATTAGTAAATAATAACCTATGTATATTATTGTGGATTGTGGGATTATTTATTTTTATTTTTATTTCATTTCTTTTCATCACAATTTTAACGTAATATCTTCACTCATAATTTTTTTGAAATGTATCCATAAATTTGTATTAAAGGTTTTCGTGTATTATGTATAAAATTCATAAAATTTATGTTAGAACATTTCCAAAGTACAGTAGAATCAAATCGAACATTTTAAAGTATAGAAGCTAAAATAAATAAAGTTGAAAGTATAGTAATCAAAATTAACCGAAACAAAAAATAAGTAGAGCTCCAATGTGTTACAGGCTAGAGGGAAAGGTAGCATTGATAACTGGTGGAGCCAGTGGCATTGGCGAATGCACGGCAAGAGTTTTTGCCCACCATGGTGCCAAAGTAGTGATTGCAGACATCCAAGACGACCTTGGCCACTCAGTTTGCAAGGTACTTGGCTCCTCCAACTCCCTCTATGTCCACTGTGACGTCACAGACGAATCCCACATAAAAAACGCCATCCACAAGGCCATCGAAGCCTTTGGAAAGTTGGACATCATGTTCAACAACGCAGGCATAGCTGACGCCAGCAAACCGCGAATCATCGACAATGAGAAAGAAGATTTCGAACGAGTGCTCAGCATAAACGTCACCGGAGTTTTCCTCGGCATCAAACACGCAGCTCAAGCAATGATCCCGACGAGAAGCGGAAGCATAATATCAACTGCAAGTGTGGCCTCATATGTTGGAGGTGCAGCCTCACATGCCTATACTTGCTCAAAGCACGCTGTGGTTGGGTTAACCAAAAATGCTGCCGTTGAGTTAGGCCAATTTGGGATTAGGGTTAATTGTTTATCTCCATATGCATTGGCCACACCTTTGGCTACAAAGTTTGTAGGGTTGGATAATGAGGAGTTTGAGAATTCAATGAACTCTAAGGCAAATTTAAAGGGTGTGACGCTTAAAGCTGAAGATGTGGCCAATGCAGCTTTGTTTTTGGCAAGCGATGAAGCGAAGTACGTGAGCGGACACAATCTATTGATCGATGGAGGTTTTGGCATCGTTAATTCGTCTATTCAAATATTTGAATACCCACAAGTTTAG

mRNA sequence

ATGGCAAGCACTGAGCTTAAGAGGCTAGAGGGAAAGGTAGCATTGATAACTGGTGGAGCCAGTGGCATTGGCGAATGCACGGCAAGAGTTTTTGCCCACCATGGTGCCAAAGTAGTGATTGCAGACATCCAAGACGACCTTGGCCACTCAGTTTGCAAGGTACTTGGCTCCTCCAACTCCCTCTATGTCCACTGTGACGTCACAGACGAATCCCACATAAAAAACGCCATCCACAAGGCCATCGAAGCCTTTGGAAAGTTGGACATCATGTTCAACAACGCAGGCATAGCTGACGCCAGCAAACCGCGAATCATCGACAATGAGAAAGAAGATTTCGAACGAGTGCTCAGCATAAACGTCACCGGAGTTTTCCTCGGCATCAAACACGCAGCTCAAGCAATGATCCCGACGAGAAGCGGAAGCATAATATCAACTGCAAGTGTGGCCTCATATGTTGGAGGTGCAGCCTCACATGCCTATACTTGCTCAAAGCACGCTGTGGTTGGGTTAACCAAAAATGCTGCCGTTGAGTTAGGCCAATTTGGGATTAGGGTTAATTGTTTATCTCCATATGCATTGGCCACACCTTTGGCTACAAAGTTTGTAGGGTTGGATAATGAGGAGTTTGAGAATTCAATGAACTCTAAGGCAAATTTAAAGGGTGTGACGCTTAAAGCTGAAGATGTGGCCAATGCAGCTTTGTTTTTGGCAAGCGATGAAGCGAAGTACGTGAGCGGACACAATCTATTGATCGATGGAGGTTTTGGCATCGTTAATTCGTCTATTCAAATATTTGAATACCCACAAGTTTAG

Coding sequence (CDS)

ATGGCAAGCACTGAGCTTAAGAGGCTAGAGGGAAAGGTAGCATTGATAACTGGTGGAGCCAGTGGCATTGGCGAATGCACGGCAAGAGTTTTTGCCCACCATGGTGCCAAAGTAGTGATTGCAGACATCCAAGACGACCTTGGCCACTCAGTTTGCAAGGTACTTGGCTCCTCCAACTCCCTCTATGTCCACTGTGACGTCACAGACGAATCCCACATAAAAAACGCCATCCACAAGGCCATCGAAGCCTTTGGAAAGTTGGACATCATGTTCAACAACGCAGGCATAGCTGACGCCAGCAAACCGCGAATCATCGACAATGAGAAAGAAGATTTCGAACGAGTGCTCAGCATAAACGTCACCGGAGTTTTCCTCGGCATCAAACACGCAGCTCAAGCAATGATCCCGACGAGAAGCGGAAGCATAATATCAACTGCAAGTGTGGCCTCATATGTTGGAGGTGCAGCCTCACATGCCTATACTTGCTCAAAGCACGCTGTGGTTGGGTTAACCAAAAATGCTGCCGTTGAGTTAGGCCAATTTGGGATTAGGGTTAATTGTTTATCTCCATATGCATTGGCCACACCTTTGGCTACAAAGTTTGTAGGGTTGGATAATGAGGAGTTTGAGAATTCAATGAACTCTAAGGCAAATTTAAAGGGTGTGACGCTTAAAGCTGAAGATGTGGCCAATGCAGCTTTGTTTTTGGCAAGCGATGAAGCGAAGTACGTGAGCGGACACAATCTATTGATCGATGGAGGTTTTGGCATCGTTAATTCGTCTATTCAAATATTTGAATACCCACAAGTTTAG

Protein sequence

MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGHNLLIDGGFGIVNSSIQIFEYPQV
Homology
BLAST of Moc05g29840 vs. NCBI nr
Match: XP_022144617.1 (secoisolariciresinol dehydrogenase-like [Momordica charantia])

HSP 1 Score: 527.7 bits (1358), Expect = 5.8e-146
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0

Query: 1   MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS 60
           MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS
Sbjct: 1   MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS 60

Query: 61  LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV 120
           LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV
Sbjct: 61  LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV 120

Query: 121 TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 180
           TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ
Sbjct: 121 TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 180

Query: 181 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 240
           FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE
Sbjct: 181 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 240

Query: 241 AKYVSGHNLLIDGGFGIVNSSIQIFEYPQV 271
           AKYVSGHNLLIDGGFGIVNSSIQIFEYPQV
Sbjct: 241 AKYVSGHNLLIDGGFGIVNSSIQIFEYPQV 270

BLAST of Moc05g29840 vs. NCBI nr
Match: XP_004136701.3 (secoisolariciresinol dehydrogenase isoform X1 [Cucumis sativus])

HSP 1 Score: 441.4 bits (1134), Expect = 5.5e-120
Identity = 223/277 (80.51%), Postives = 246/277 (88.81%), Query Frame = 0

Query: 1   MASTEL-------KRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVC- 60
           MAS E+       +RLEGKVALITGGASGIGECTA++F HHGAKVVIADIQDDLGH++C 
Sbjct: 1   MASNEIISTTTWQRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCA 60

Query: 61  KVLGSSNSLYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDF 120
            VLGS+NSLYVHCDVT+ES ++ A+  A+E FGKLDIM NNAGIAD SKPRIIDN+K DF
Sbjct: 61  NVLGSTNSLYVHCDVTEESQVQEAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDF 120

Query: 121 ERVLSINVTGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTK 180
           +RVLSINVTGVFLGIKHAAQAMIP ++GSI+STASVASY GG+ASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLSINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYTGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSP+AL TPLATKFVGLD  EFE  M SKANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPFALVTPLATKFVGLDGPEFEKIMGSKANLKGVTLKAEDVANA 240

Query: 241 ALFLASDEAKYVSGHNLLIDGGFGIVNSSIQIFEYPQ 270
           ALFLASDE++YVSGHNL IDGGF IVN +I IF+YPQ
Sbjct: 241 ALFLASDESRYVSGHNLFIDGGFSIVNPNIDIFQYPQ 277

BLAST of Moc05g29840 vs. NCBI nr
Match: XP_023891279.1 (secoisolariciresinol dehydrogenase-like [Quercus suber])

HSP 1 Score: 441.4 bits (1134), Expect = 5.5e-120
Identity = 221/268 (82.46%), Postives = 243/268 (90.67%), Query Frame = 0

Query: 1   MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS 60
           + S   +RLEGKVALITGGASGIGE TA+VF HHGAKVVIADIQDDLGHSV + LGSSN+
Sbjct: 6   LISAVTRRLEGKVALITGGASGIGEFTAKVFVHHGAKVVIADIQDDLGHSVIEALGSSNA 65

Query: 61  LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV 120
           LYVHCDVTDE+ IKNA+ KA+  +GKLDIMFNNAGIAD +K RIIDNEK DFERVLS+NV
Sbjct: 66  LYVHCDVTDEAQIKNAVDKAVATYGKLDIMFNNAGIADENKARIIDNEKADFERVLSVNV 125

Query: 121 TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 180
           TGVFLGIKHA+QAMIP RSG IIST+S++SYVGGAAS+AYTCSKHAV+GLTKNAAVELGQ
Sbjct: 126 TGVFLGIKHASQAMIPARSGCIISTSSISSYVGGAASYAYTCSKHAVLGLTKNAAVELGQ 185

Query: 181 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 240
           FGIRVNCLSPYALATPLATKFVG+D+E  EN MNS ANLKGVTLKAEDVANAALFLASDE
Sbjct: 186 FGIRVNCLSPYALATPLATKFVGVDDETLENMMNSLANLKGVTLKAEDVANAALFLASDE 245

Query: 241 AKYVSGHNLLIDGGFGIVNSSIQIFEYP 269
           +KYVSGHNLLIDGGF IVN S  +F+YP
Sbjct: 246 SKYVSGHNLLIDGGFSIVNPSFNMFQYP 273

BLAST of Moc05g29840 vs. NCBI nr
Match: XP_038903548.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 440.7 bits (1132), Expect = 9.4e-120
Identity = 218/269 (81.04%), Postives = 247/269 (91.82%), Query Frame = 0

Query: 2   ASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCK-VLGSSNS 61
           A+T  +RLEGKVALITGGASGIGECTA++F HHGAKVVIAD+QDDLGH++C  +LG +NS
Sbjct: 9   AATGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADVQDDLGHALCNTILGPTNS 68

Query: 62  LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV 121
           LYVHCDVTDES ++ A+  A++ FGKLDIM NNAGIAD SKPRIIDNEK DF+RVLS+NV
Sbjct: 69  LYVHCDVTDESQVQAAVVAAVKTFGKLDIMMNNAGIADPSKPRIIDNEKHDFDRVLSVNV 128

Query: 122 TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 181
           TGVFLGIKHAAQAMIP ++GSI+STASVASYVGG+ASHAYTCSKHAVVGLTKNAAVELGQ
Sbjct: 129 TGVFLGIKHAAQAMIPAKTGSILSTASVASYVGGSASHAYTCSKHAVVGLTKNAAVELGQ 188

Query: 182 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 241
           FGIRVNCLSPYAL TPLATKF  L+ EEFE +MNS ANLKGVTLKA+DVANAALFLASDE
Sbjct: 189 FGIRVNCLSPYALVTPLATKFTRLEGEEFEMAMNSMANLKGVTLKAQDVANAALFLASDE 248

Query: 242 AKYVSGHNLLIDGGFGIVNSSIQIFEYPQ 270
           ++YVSGHNLLIDGGFGIVN +I+IF+YP+
Sbjct: 249 SRYVSGHNLLIDGGFGIVNPNIKIFQYPE 277

BLAST of Moc05g29840 vs. NCBI nr
Match: XP_031737878.1 (secoisolariciresinol dehydrogenase isoform X2 [Cucumis sativus])

HSP 1 Score: 440.3 bits (1131), Expect = 1.2e-119
Identity = 220/265 (83.02%), Postives = 241/265 (90.94%), Query Frame = 0

Query: 6   LKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVC-KVLGSSNSLYVH 65
           L RLEGKVALITGGASGIGECTA++F HHGAKVVIADIQDDLGH++C  VLGS+NSLYVH
Sbjct: 9   LIRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCANVLGSTNSLYVH 68

Query: 66  CDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVF 125
           CDVT+ES ++ A+  A+E FGKLDIM NNAGIAD SKPRIIDN+K DF+RVLSINVTGVF
Sbjct: 69  CDVTEESQVQEAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDFDRVLSINVTGVF 128

Query: 126 LGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIR 185
           LGIKHAAQAMIP ++GSI+STASVASY GG+ASHAYTCSKHAVVGLTKNAAVELGQFGIR
Sbjct: 129 LGIKHAAQAMIPAKTGSILSTASVASYTGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR 188

Query: 186 VNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYV 245
           VNCLSP+AL TPLATKFVGLD  EFE  M SKANLKGVTLKAEDVANAALFLASDE++YV
Sbjct: 189 VNCLSPFALVTPLATKFVGLDGPEFEKIMGSKANLKGVTLKAEDVANAALFLASDESRYV 248

Query: 246 SGHNLLIDGGFGIVNSSIQIFEYPQ 270
           SGHNL IDGGF IVN +I IF+YPQ
Sbjct: 249 SGHNLFIDGGFSIVNPNIDIFQYPQ 273

BLAST of Moc05g29840 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 2.7e-101
Identity = 181/263 (68.82%), Postives = 218/263 (82.89%), Query Frame = 0

Query: 7   KRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCD 66
           +RLEGKVALITGGASGIGE TA++F+ HGAKV IAD+QD+LGHSV + +G+SNS Y+HCD
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCD 72

Query: 67  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 126
           VT+E  +KNA+   +  +GKLDIMF+NAGI+D ++PRIIDNEK DFERV S+NVTGVFL 
Sbjct: 73  VTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFLC 132

Query: 127 IKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 186
           +KHAA+ MIP RSG+IISTAS++S +GG +SHAY  SKHAV+GLT+N AVELGQFGIRVN
Sbjct: 133 MKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVN 192

Query: 187 CLSPYALATPLATKFVGLDN-EEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVS 246
           CLSP+ L T L  KF G+ N EEFEN +N   NLKG     EDVANAAL+LASDEAKYVS
Sbjct: 193 CLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASDEAKYVS 252

Query: 247 GHNLLIDGGFGIVNSSIQIFEYP 269
           GHNL IDGGF + NS I++F+YP
Sbjct: 253 GHNLFIDGGFSVCNSVIKVFQYP 275

BLAST of Moc05g29840 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 5.6e-83
Identity = 159/264 (60.23%), Postives = 198/264 (75.00%), Query Frame = 0

Query: 3   STELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLY 62
           S + ++L GKVA+ITGGASGIG CTAR+F  HGA+VV+ADIQD+LG S+   LG   S Y
Sbjct: 9   SADARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSY 68

Query: 63  VHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTG 122
           VHCDVT+E  +  A+  A+  FGKLD+MFNNAG++     R+ +  KEDFERVL++N+ G
Sbjct: 69  VHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVG 128

Query: 123 VFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFG 182
            FLG KHAA+ M P R GSIISTAS++S V GAASHAYT SKHA+VG T+NAA ELG+ G
Sbjct: 129 PFLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHG 188

Query: 183 IRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGV-TLKAEDVANAALFLASDEA 242
           IRVNC+SP  +ATPLA   +G+D+E  E  M + ANLKG   LKA+D+A AALFLASD+ 
Sbjct: 189 IRVNCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDG 248

Query: 243 KYVSGHNLLIDGGFGIVNSSIQIF 266
           +YVSG NL +DGG  +VNSS   F
Sbjct: 249 RYVSGQNLRVDGGLSVVNSSFGFF 272

BLAST of Moc05g29840 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 6.4e-71
Identity = 136/259 (52.51%), Postives = 179/259 (69.11%), Query Frame = 0

Query: 3   STELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSL- 62
           ++   RL+ KVA+ITGGA GIGE TA++F  +GAKVVIADI DD G  VC  +GS + + 
Sbjct: 8   ASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVIS 67

Query: 63  YVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVT 122
           +VHCDVT +  ++N +   I   GKLDIMF N G+   +   I++   EDF+RV+ INV 
Sbjct: 68  FVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVY 127

Query: 123 GVFLGIKHAAQAMIPTRSGSIISTASVASYVGG-AASHAYTCSKHAVVGLTKNAAVELGQ 182
           G FL  KHAA+ MIP + GSI+ TAS++S+  G   SH YT +KHAV+GLT +   ELGQ
Sbjct: 128 GAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQ 187

Query: 183 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 242
            GIRVNC+SPY +A+PL T   G+D+   E   +  ANLKG+ L+AEDVA+A  +LA DE
Sbjct: 188 HGIRVNCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKGILLRAEDVADAVAYLAGDE 247

Query: 243 AKYVSGHNLLIDGGFGIVN 260
           +KYVSG NL+IDGG+   N
Sbjct: 248 SKYVSGLNLVIDGGYTRTN 266

BLAST of Moc05g29840 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 6.4e-71
Identity = 142/266 (53.38%), Postives = 182/266 (68.42%), Query Frame = 0

Query: 8   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSS-NSLYVHCD 67
           RLEGKVAL+TGGASGIGE  AR+F  HGAK+ I D+QD+LG  V + LG   ++ Y HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 68  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 127
           VT E  ++ A+    E +G +DIM NNAGI       I D +  +F++V  INV GVFLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 128 IKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 187
           +KHAA+ MIP   GSI+S ASV+S + GA  H YT +KHAVVGLTK+ A ELG+ GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 188 CLSPYALATPLATKFVGLDNEE------FENSMNSKANLKGVTLKAEDVANAALFLASDE 247
           C+SPYA+ T L+  ++     +      F   + S ANLKGV L   DVA A L+LA++E
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLATEE 241

Query: 248 AKYVSGHNLLIDGGFGIVNSSIQIFE 267
           +KYVSG NL+IDGGF I N ++Q+FE
Sbjct: 242 SKYVSGLNLVIDGGFSIANHTLQVFE 267

BLAST of Moc05g29840 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.4e-70
Identity = 135/254 (53.15%), Postives = 177/254 (69.69%), Query Frame = 0

Query: 8   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSL-YVHCD 67
           RL+ KVA+ITGGA GIGE TA++F  +GAKVVIADI DD G  VC  +GS + + +VHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 68  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 127
           VT +  ++N +   I   GKLDIMF N G+   +   I++   EDF+RV+ INV G FL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 128 IKHAAQAMIPTRSGSIISTASVASYVGG-AASHAYTCSKHAVVGLTKNAAVELGQFGIRV 187
            KHAA+ MIP + GSI+ TAS++S+  G   SH YT +KHAV+GLT +   ELG++GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRV 192

Query: 188 NCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVS 247
           NC+SPY +A+PL T   G+D+   E   +  ANLKG  L+AEDVA+A  +LA DE+KYVS
Sbjct: 193 NCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKGTLLRAEDVADAVAYLAGDESKYVS 252

Query: 248 GHNLLIDGGFGIVN 260
           G NL+IDGG+   N
Sbjct: 253 GLNLVIDGGYTRTN 266

BLAST of Moc05g29840 vs. ExPASy TrEMBL
Match: A0A6J1CU62 (secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111014261 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 2.8e-146
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0

Query: 1   MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS 60
           MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS
Sbjct: 1   MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS 60

Query: 61  LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV 120
           LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV
Sbjct: 61  LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV 120

Query: 121 TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 180
           TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ
Sbjct: 121 TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 180

Query: 181 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 240
           FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE
Sbjct: 181 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 240

Query: 241 AKYVSGHNLLIDGGFGIVNSSIQIFEYPQV 271
           AKYVSGHNLLIDGGFGIVNSSIQIFEYPQV
Sbjct: 241 AKYVSGHNLLIDGGFGIVNSSIQIFEYPQV 270

BLAST of Moc05g29840 vs. ExPASy TrEMBL
Match: A0A7N2N7S8 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 7.8e-120
Identity = 220/268 (82.09%), Postives = 244/268 (91.04%), Query Frame = 0

Query: 1   MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS 60
           + S   +RLEGKVALITGGASGIGE TA+VF HHGAKVVIADIQD+LGHSV + LGSSN+
Sbjct: 6   LISAVTRRLEGKVALITGGASGIGEFTAKVFVHHGAKVVIADIQDELGHSVIEALGSSNA 65

Query: 61  LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV 120
           LYVHCDVTDE+ IKNA+ KA+  +GKLDIMFNNAGIAD +K RIIDNEK DFERVLS+NV
Sbjct: 66  LYVHCDVTDEAQIKNAVDKAVATYGKLDIMFNNAGIADENKVRIIDNEKADFERVLSVNV 125

Query: 121 TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 180
           TGVFLGIKHA+QAMIP RSGSIIST+S++SYVGGAAS++YTCSKHAVVGLTKNAAVELGQ
Sbjct: 126 TGVFLGIKHASQAMIPARSGSIISTSSISSYVGGAASYSYTCSKHAVVGLTKNAAVELGQ 185

Query: 181 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 240
           FGIRVNCLSPYALATPLATKFVG+D+E  EN MNS ANLKGVTLKAEDVANAALFLASDE
Sbjct: 186 FGIRVNCLSPYALATPLATKFVGVDDETLENVMNSLANLKGVTLKAEDVANAALFLASDE 245

Query: 241 AKYVSGHNLLIDGGFGIVNSSIQIFEYP 269
           ++YVSGHNLLIDGGF IVN S  +F+YP
Sbjct: 246 SRYVSGHNLLIDGGFSIVNPSFNMFQYP 273

BLAST of Moc05g29840 vs. ExPASy TrEMBL
Match: A0A7N2M6N8 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 5.0e-119
Identity = 219/268 (81.72%), Postives = 243/268 (90.67%), Query Frame = 0

Query: 1   MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS 60
           + S   +RLEGKVALITGGASGIGE TA+VF HHGAKVVIADIQD+LGHSV +  GSSN+
Sbjct: 6   LISAVTRRLEGKVALITGGASGIGEFTAKVFVHHGAKVVIADIQDELGHSVIEAHGSSNA 65

Query: 61  LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV 120
           LYVHCDVTDE+ IKNA+ KA+  +GKLDIMFNNAGIAD +K RIIDNEK DFERVLS+NV
Sbjct: 66  LYVHCDVTDEAQIKNAVDKAVATYGKLDIMFNNAGIADENKVRIIDNEKADFERVLSVNV 125

Query: 121 TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 180
           TGVFLGIKHA+QAMIP RSGSIIST+S++SYVGGAAS++YTCSKHAVVGLTKNAAVELGQ
Sbjct: 126 TGVFLGIKHASQAMIPARSGSIISTSSISSYVGGAASYSYTCSKHAVVGLTKNAAVELGQ 185

Query: 181 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 240
           FGIRVNCLSPYALATPLATKFVG+D+E  EN MNS ANLKGVTLKAEDVANAALFLASDE
Sbjct: 186 FGIRVNCLSPYALATPLATKFVGVDDETLENVMNSLANLKGVTLKAEDVANAALFLASDE 245

Query: 241 AKYVSGHNLLIDGGFGIVNSSIQIFEYP 269
           ++YVSGHNLLIDGGF IVN S  +F+YP
Sbjct: 246 SRYVSGHNLLIDGGFSIVNPSFNMFQYP 273

BLAST of Moc05g29840 vs. ExPASy TrEMBL
Match: A0A6J1J123 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111481719 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.1e-118
Identity = 217/270 (80.37%), Postives = 243/270 (90.00%), Query Frame = 0

Query: 2   ASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVL--GSSN 61
           A T  +RLEGKVA+ITGGASGIGECTA++F HHGAKVVIADIQDDLG ++C     G +N
Sbjct: 8   APTAPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQALCATALGGPTN 67

Query: 62  SLYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSIN 121
            LYVHCDVTDE+ IK A+  A+EAFGKLDIM NNAGIAD SKPRIIDN KEDFERVL+IN
Sbjct: 68  CLYVHCDVTDEAQIKAAVEAAVEAFGKLDIMMNNAGIADPSKPRIIDNHKEDFERVLNIN 127

Query: 122 VTGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELG 181
           VTGVFLGIKHAAQAMIP ++GSIISTAS+ASYVGG+ASHAYTCSKHAVVGLTKNAAVELG
Sbjct: 128 VTGVFLGIKHAAQAMIPVKTGSIISTASLASYVGGSASHAYTCSKHAVVGLTKNAAVELG 187

Query: 182 QFGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASD 241
           QFGIRVNCLSP+A+ TPLAT+FVGL+ EEFE +MNS ANLKGVTL+ EDVANAALFLASD
Sbjct: 188 QFGIRVNCLSPFAMVTPLATRFVGLEGEEFETAMNSMANLKGVTLRTEDVANAALFLASD 247

Query: 242 EAKYVSGHNLLIDGGFGIVNSSIQIFEYPQ 270
           E++YVSGHNLLIDGGFGI N +I+IFEYP+
Sbjct: 248 ESRYVSGHNLLIDGGFGITNPNIKIFEYPE 277

BLAST of Moc05g29840 vs. ExPASy TrEMBL
Match: W9RXQ4 (Momilactone A synthase OS=Morus notabilis OX=981085 GN=L484_018868 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.5e-118
Identity = 214/269 (79.55%), Postives = 244/269 (90.71%), Query Frame = 0

Query: 1   MASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS 60
           + S   +RLEGKVALITGGASGIGECTA+VFAHHGAK+VIADIQDDLGHSVC+ LG +NS
Sbjct: 6   LLSAVSRRLEGKVALITGGASGIGECTAKVFAHHGAKLVIADIQDDLGHSVCESLGQTNS 65

Query: 61  LYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINV 120
            YVHC+VTDESH++NA+ +A++ +G+LDIMFNNAGIAD +K RIID+EK DFERV+S+NV
Sbjct: 66  KYVHCNVTDESHVENAVDEAVKTYGRLDIMFNNAGIADENKARIIDSEKADFERVMSVNV 125

Query: 121 TGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 180
           TGVFLGIKHAAQAMIP+RSGSIISTAS++SYVGGAASHAY CSKHAV+GLTKNAAVELGQ
Sbjct: 126 TGVFLGIKHAAQAMIPSRSGSIISTASISSYVGGAASHAYCCSKHAVIGLTKNAAVELGQ 185

Query: 181 FGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 240
           FGIRVN LSPYALATPLATKFVGLD+E  EN MNS  NLK VTLKAEDVANAAL+LASDE
Sbjct: 186 FGIRVNSLSPYALATPLATKFVGLDDEGLENLMNSLGNLKHVTLKAEDVANAALYLASDE 245

Query: 241 AKYVSGHNLLIDGGFGIVNSSIQIFEYPQ 270
            +Y+SGHNLLIDGGF IVN S  +F+YP+
Sbjct: 246 GRYISGHNLLIDGGFSIVNPSFNMFQYPK 274

BLAST of Moc05g29840 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 5.6e-70
Identity = 143/276 (51.81%), Postives = 178/276 (64.49%), Query Frame = 0

Query: 2   ASTELKRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVL----GS 61
           +S   +RL GKVALITGGA+GIGE   R+F  HGAKV I D+QDDLG  VCK L      
Sbjct: 11  SSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESK 70

Query: 62  SNSLYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLS 121
             + ++H DV  E  I NA+  A++ FG LDI+ NNAG+  A  P I +    +FE    
Sbjct: 71  ETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFD 130

Query: 122 INVTGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVE 181
           +NV G FL +KHAA+ MIP + GSI+S  SV   VGG   H+Y  SKHAV+GLT++ A E
Sbjct: 131 VNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAE 190

Query: 182 LGQFGIRVNCLSPYALATPLATKFVGLDNEE--------FENSMNSKANLKGVTLKAEDV 241
           LGQ GIRVNC+SPYA+AT LA     L  EE        F N   + ANLKGV L  +DV
Sbjct: 191 LGQHGIRVNCVSPYAVATKLA--LAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDV 250

Query: 242 ANAALFLASDEAKYVSGHNLLIDGGFGIVNSSIQIF 266
           ANA LFLASD+++Y+SG NL+IDGGF   N S ++F
Sbjct: 251 ANAVLFLASDDSRYISGDNLMIDGGFTCTNHSFKVF 284

BLAST of Moc05g29840 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 253.1 bits (645), Expect = 2.6e-67
Identity = 136/264 (51.52%), Postives = 181/264 (68.56%), Query Frame = 0

Query: 7   KRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS----LY 66
           KRLEGKVA+ITGGA GIG+ T  +FA HGA VVIAD+ +  G S+ K L S  +     +
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 67  VHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPR--IIDNEKEDFERVLSINV 126
           + CDV+ E+ ++N ++  +  +G+LDI+FNNAG+    K    I+D + ++F+ V+ +NV
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 127 TGVFLGIKHAAQAMIPTR-SGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELG 186
            GV LG+KH A+AMI     G IISTASVA  +GG   HAYT SKHA+VGLTKNAA ELG
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 187 QFGIRVNCLSPYALATPLATK---------FVGLDNEEFENSMNSKANLKGVTLKAEDVA 246
           ++GIRVNC+SP+ +AT +                D EE E  + S ANLKG TL+A D+A
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIA 269

Query: 247 NAALFLASDEAKYVSGHNLLIDGG 255
            AAL+LASDE+KYV+GHNL++DGG
Sbjct: 270 EAALYLASDESKYVNGHNLVVDGG 293

BLAST of Moc05g29840 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 3.5e-64
Identity = 121/253 (47.83%), Postives = 175/253 (69.17%), Query Frame = 0

Query: 7   KRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCD 66
           KRL+GK+ +ITGGASGIG  + R+F  HGA+VVI D+QD+LG +V   +G   + Y HCD
Sbjct: 4   KRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCD 63

Query: 67  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 126
           VT+E+ ++NA+   +E +GKLD++F+NAG+ +     I+D    + +R ++IN+ G    
Sbjct: 64  VTNETEVENAVKFTVEKYGKLDVLFSNAGVIEPF-VSILDLNLNELDRTIAINLRGTAAF 123

Query: 127 IKHAAQAMIPTR-SGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRV 186
           IKHAA+AM+     GSI+ T SVA+ + G A H YT SKH ++GL K+A+  LG++GIRV
Sbjct: 124 IKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRV 183

Query: 187 NCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVS 246
           N ++P+ +ATPL      ++    E + ++ ANLKG+ LKA  VA AALFLASDE+ YVS
Sbjct: 184 NGVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESAYVS 243

Query: 247 GHNLLIDGGFGIV 259
           G NL +DGG+ +V
Sbjct: 244 GQNLAVDGGYSVV 255

BLAST of Moc05g29840 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-62
Identity = 127/254 (50.00%), Postives = 166/254 (65.35%), Query Frame = 0

Query: 7   KRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCD 66
           K+LEGKVALITGGASG+G+ TA  F  HGA+VVIAD+  + G    K LG S + +V CD
Sbjct: 39  KKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELG-SEAEFVRCD 98

Query: 67  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDN-EKEDFERVLSINVTGVFL 126
           VT E+ I  A+   +E +GKLD+M+NNAGI     P  I   +  +FERV+ INV GV  
Sbjct: 99  VTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFGVVS 158

Query: 127 GIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRV 186
           GIKHAA+ MIP RSG I+ T+SVA   GG A H+YT SK    G+ K+AA EL + G+R+
Sbjct: 159 GIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVRI 218

Query: 187 NCLSPYALATPLATK-----FVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDE 246
           NC+SP  +ATPL        F  +  E+   ++     LKG   +  DVA AAL+LAS++
Sbjct: 219 NCISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELKGAECEEADVAKAALYLASND 278

Query: 247 AKYVSGHNLLIDGG 255
            KYV+GHNL++DGG
Sbjct: 279 GKYVTGHNLVVDGG 291

BLAST of Moc05g29840 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 236.5 bits (602), Expect = 2.5e-62
Identity = 128/259 (49.42%), Postives = 168/259 (64.86%), Query Frame = 0

Query: 7   KRLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCD 66
           ++LEGKVALITGGASGIG+ TA  F  HGAKV+IADIQ  +G    + LG S   Y  CD
Sbjct: 76  RKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELGPS-CAYFPCD 135

Query: 67  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 126
           VT ES I NA+  A+    KLDIM+NNAGI   + P I+D +   F++V++ NV GV  G
Sbjct: 136 VTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVINTNVRGVMAG 195

Query: 127 IKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 186
           IKHAA+ MIP  SGSII   SV   +GG A H Y+ SK AV+G+ ++ A EL +  IRVN
Sbjct: 196 IKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRIRVN 255

Query: 187 CLSPYALATPLATK-----FVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEA 246
           C+SP+A+ T          + G+D+      + S   L G   +  DVANAA++LASD++
Sbjct: 256 CISPFAITTSFVMDEMRQIYPGVDDSRLIQIVQSTGVLNGEVCEPTDVANAAVYLASDDS 315

Query: 247 KYVSGHNLLIDGGFGIVNS 261
           KYV+GHNL++DGGF  V +
Sbjct: 316 KYVNGHNLVVDGGFTTVKT 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144617.15.8e-146100.00secoisolariciresinol dehydrogenase-like [Momordica charantia][more]
XP_004136701.35.5e-12080.51secoisolariciresinol dehydrogenase isoform X1 [Cucumis sativus][more]
XP_023891279.15.5e-12082.46secoisolariciresinol dehydrogenase-like [Quercus suber][more]
XP_038903548.19.4e-12081.04secoisolariciresinol dehydrogenase-like [Benincasa hispida][more]
XP_031737878.11.2e-11983.02secoisolariciresinol dehydrogenase isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q94KL72.7e-10168.82Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
Q7FAE15.6e-8360.23Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ... [more]
A3F5F06.4e-7152.51Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ... [more]
F1SWA06.4e-7153.38Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Q94KL82.4e-7053.15Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
Match NameE-valueIdentityDescription
A0A6J1CU622.8e-146100.00secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A7N2N7S87.8e-12082.09Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A7N2M6N85.0e-11981.72Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A6J1J1231.1e-11880.37secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111481... [more]
W9RXQ41.5e-11879.55Momilactone A synthase OS=Morus notabilis OX=981085 GN=L484_018868 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52340.15.6e-7051.81NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G51680.12.6e-6751.52NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47140.13.5e-6447.83NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G26770.11.1e-6250.00NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G03140.12.5e-6249.42NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 140..148
score: 46.12
coord: 85..96
score: 51.1
coord: 160..179
score: 37.39
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 219..239
score: 34.88
coord: 134..150
score: 33.53
coord: 160..179
score: 42.87
coord: 181..198
score: 37.48
coord: 13..30
score: 49.89
coord: 85..96
score: 58.12
NoneNo IPR availableGENE3D3.40.50.720coord: 1..265
e-value: 5.1E-83
score: 280.2
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 18..255
e-value: 1.6E-69
score: 234.0
NoneNo IPR availablePIRSRPIRSR000094-2PIRSR000094-2coord: 8..260
e-value: 2.4E-24
score: 83.7
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 15..208
e-value: 9.5E-20
score: 68.8
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 8..260
e-value: 2.4E-24
score: 83.7
NoneNo IPR availablePANTHERPTHR43180:SF50SECOISOLARICIRESINOL DEHYDROGENASE-LIKEcoord: 3..267
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 3..267
NoneNo IPR availableCDDcd05326secoisolariciresinol-DH_like_SDR_ccoord: 8..255
e-value: 4.57326E-123
score: 349.062
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 147..175
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 10..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g29840.1Moc05g29840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity