Moc05g27540 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g27540
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	NADH-cytochrome b5 reductase
Location	chr5: 19898350 .. 19903698 (+)
RNA-Seq Expression	Moc05g27540
Synteny	Moc05g27540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATCTTACACACGCTCGACTTTGAGGTTCTTGTCAGTTTGGGAGTGGTCTTGGCTACCTTTGGTCTTGGTGCCGTCTTCATCGTTTTGTCCAGAAAACGCGCATCCAGAGGTTTCGCCTCCTTCTCATTTGATTCTTCGAAATGGGATCTCTGTTTTCTGGTTTGATTTGTTGGGTGTTTTCAATTCCTGATGGATGGTTTGATGTTTTCATTCGTGGGTCGTTACAATCTATGCTGATGGTTTCATGGACTGTTATTTATTTTTCCTCTTGATGTTTTAATGGATATATGGCACGTCTGCAATTTGAAATTAGATTCTGTTTTAGTTTTTATTTCTACTCTAGTCATTTTTACTCTTTCCGGCTGGAAAGTGAGTGAATGGTGTGGAATAAGTGTTTGTTGAAAAGTCTGTATAGCGAGTCTAGCGTAGAAGGGGTAGCATTCATGGGAAAAGTCAAGTGCTTCACTGGGCTTTTGGACGTGCTTAAATTTCAAAATAGCTGATTGAACTAAAATTCAAATGGCCATGGAGAGTTTCTTCATTTGCGTTGAGCTCTTGGTCAATATGAAATGGTAGATGTTATGGAAAATATGCGTTTTCTAGAACTCAAAGTTCTTGGTTCTTTGCTTGTCTTGTCTCTTGTGGGAATTTTAACACCTATGATGTTATCTCAGTAGCAGCCCCCCTGATGAGACAAGGTTGCAGTAGGAATGGTCTTTAACACTTGATTTTGCACATTCCATTATTCAAACTTCGGATTAATTGTAGGAACAGTGTTAGTCGGTGAACTTTGTGGTAGAAACGGCTCTAGTTGAGGATTCTATTCATCAATAAAGCATATGTGGTGACATAGGTCCAAAAGATCTTCCCCTTTTTTTTGTGACTTCAATAGCGTGTTCTCTGCAAACATGAGTGTAAGGTTTTAAATATTTTATCCTGGACATGCTCAAATTTAGGGAAGATTTGATAAGAGTTTTTCCCCCCTTCTCTTCATAAAGGTTTTTGAGTTGTATTTTGCTTCCCTCTCCATTCTGGTTACATGTTTATATGGAAGTAATTCTTTACTCTCCTTTTTGTCATAGAGGCTGCTTTCTTTTACAAACCGGTTCTTTGGTAGTCTTATGACACATTTGATCAAAAGGAGTAGGAGGATCTTTGATTACAAACTATCGGAGCCTTTTCTTGTAGTTATTGTTTCTTACTTCATGTTGGCGTGTCTTTGGTATAGAATCCTGTACTTTAATTCCTCTTAATGAAAGCAGTTTTTTTGAGAGGAAAAAGAAACACCTTGGTTAGGCTTACTGCTTTAGAGCTATTTGCCTGGCTTCTGCGGTTAATACTTAGTTTGTGTGTTTCTAGACTTTTCAAATCCTTGACCAGTGCTTTTAATGATCAACGTACCTCCTATACTAACATTCTCTTCTGGTTGTCATGGTTTTGTTCAGAGATAATTTAGGAACCATATTTTTTCTTGATTCCTATCTAAAAAATTTTCTTTTTCTGCATTCTATTTCTTTATTTATTTACTTTTTTCTAACGTGGATTGGCTCATATGTTCCATGTTGTTTGTAACTACAGCCTGCTTGGATCCAGAGAAGTTTAAAGAGTTTAAGCTTGTTAAGCGTACGAAGCTGAGCCATAATGTGGCGAAGTTCACTTTTATACTTCCCCGTCCCAATTCAATTCTTGGTCTTCCTATTGGACAACATATAAGTTGCAGGTTTCATTTCATCCAATATATTTAGTTTCCCAGTCATCATCTTGATAGTTTTATATACAGTTATGATACTTAGCACACCTAATATTTGGTTTATCTGTCCTGCCTTGTAACATCTCGCTCTCTCAAAAATTATGAGCAGGGGAAAGGATAGCCAGGGTGAAGAGGTTATTAAACCTTACACGCCAACTACTTTGGACTCTGATGTTGGATATTTTGAACTAGTCATAAAGGTACATATATAAATATGCCCACAGGTTGGTGATGGCACATTGATTTTTATTATTACAGTGCCCATTCTTATCTGAATGTGTGTATGAAATTTAGATGTATCCCCAAGGCAGGATGTCACATCATTTTCGAGAAATGCGTATCGGGGACCATCTTGCTGTGAAGGGACCAAAGGTAATTGCTTACTTGCAGAGAAATTATGATGACAGTTTTAGAATGTTCTATTATTCTACGTACTGCATGTGCCCAATGGCCACATGATTTCATATTATTAATCAGTTTTGTGAGAAGCTCTAGATCCAAATTTCTCCATATCACCTTGAATGTTATGATGCTAGTAACCAAGAAGTAATGCGGAAGTTTTGTTTCAGTGACTTGTGAATTTCCATTAATCTGGATTGAACACAATTGTGATTGTGATCTACATTATGTTTTAGAGTATTAAAATTGTTAATCTAAAGAATGCTTTATCCTCTCGGTCCTGATTACTTTTTGTGTCATATTTTTTCTATTAAGATATTGTCTTGTTCATGACAAAGGAGACATGTAGCGTTATTGTGTTTTTGAAGTCTTCACATAATTCTATATGCATCTATGTAGCATTATTGTGTTTTTGATTTCCACACAGACTTCAACAACACCTTACTAGCTCTTGGAAAAATTGATCTTGCTTTCACCAAAGAGTTAACTGAAAAGCAGCCAATAGGGTCTAACTTCCACACCCTTTTACCACCTCTAGAAGACAAAGAAACACCTTCCAACAATTGAGAAACTTAACATTACTAGGGCCTAGTTCAGAATTTATCACCATGGTTCTCTAATAACTTGTGTTCTTTATAGAATTTTGAATGCTTCGAATGGTGCCTAGTTTAGCTATTTATGTACTTTATTTATTGTTGCAGGGACGTTTTAAGTATCAACCTGGTCAAGTTAGAGCATTTGGGATGCTTGCTGGAGGCTCTGGCATTACACCTATGTACCAAGTATATCAAAAAAAATAAATTTTATGGAATATGTTTTTCCTAATCATTTAAAATCTTTGCTGCATTATGTTGTGTGCCATTTTATCTTGCCAAGTTTGAAAACAATAGGAAGCTCTCTCATTCTTACTTTTTGTTTTAGGTTGCTCGTGCTATATTAGAAAACCCGAAAGACGAGACTAAGGTTCACCTTATTTACGCCAATGTTACATACGAAGATATTCTTTTGAAGGTAATTTTTTGTAAAAGCTTTATATCATTTTATTGTCCTTCCATTTCCGATACTTATGTTTTTGTTGTTGTTGTTTGTGCTTGTAATGTACCATGACCACGACCACGAGTTTGTGAAGCTTGGAAAAAATGTCTTTCCCTTTTGCTGACAGTACACTTGCTAGAACAGATAATCTATATTTCAACTCGAACCTATTTGATTAATTACTTCACTTTTTCATGCATTCTCCATTTTTTAGTGTAATTATTTTAACTTTTCACTCTTGCTGTATGGTGATTACACTTCGGTTTTAATGAAAAAAAATACACGTCGGTATTTTCACAGGAGGAATTGGATATTCTTGCCACTAGATACCCTGATCGCTTCAAGATCTACTATGTGTTGAACCAGGTCAGTTAAAAGTTTTAAAATTTAGTAGTAGTCCAATATCAATAGCAAGAGATCTTAATTTTAAATCTCTGCAATATTACTTTCCTTGCGTCGTATTAACAATCCAAATGTTTAGTCTTGTGGATCAAACTAATATCAATATTAGGTTCCATCTCAAAATCAATTGGCAAGGAGAGAGATAACCCATGTATATTATAAACTTTTTTACGTTCTTTATTTTTTCAATATGGGATCCTTAACATGCCCCTTAAGATAGTGGCTCTTTTGAGCCATACCAATCTTGGGCAAATTCTATTTTTTTTTTTGGAACGGACGCTCGTTTGAGCTTTTGGGCTATGATACCATATTAGTATTCATGAGGTGTCATCTCAAAACCAATTGATAATGAGAGAAGTAACCCATGTATATTACAAACCTTTGTTAAATTATAAATTTTTGTTAAGTCTCTATTTTTTAAAAATGTGGGATCCTTAACAACGGATGAGGAAGGGTACTAAGAATATACTAAATGGTTTTGTTTGTTAGAAGTTTTGTGTCATGTTTAATGTTACTACTGATGCCTAAGTTCTTTGACAATGGAAACAGCCTCCTGAAGTTTGGGATGGTGGTGTTGGCTTTGTGTCCAAGGAAATGATTCAAGCGCATTGCCCTGCACCTGCTCCTGGCATTCAGGTATATTTTTCCCTTGAAAGTTTTAAGTTCTATTATAGATCCCTAAATTTTGGCGTTTATTTTTCTTTGGTCCTTCAATTTTTAAAACATCGTCTTAGTCTTTGAATTTTGTATAAAATGATAAAATCATTTTAGTCATTCTACCTAATCAATAAATATCTTTGTAATCAATAGATATCTATATAAACAGTGTCTGAAGTCACGTCTATATTAGGTTTTTTTCTCTTTAGTTCAAGAAAATGTGACATGCGGAGATCTAAGAAGCATGAACACTTCAATTTGGGTAACGTGCCCGTATCGGACACTCCAATACTCTAACACTTATCAAAAACACTTGTTAGCTCAATAGATGTGCGTTGTACACTAGTTGTACAAAGTAAACATGAATTCAACACTGGTTAAAAGCACATCGAACACTTGTTAAGCATAAGAAACAGATATATCTAGTAATATAGGACAAAATAATAAAATTTGAGAGCAAAATATATAAAATCATTTTTTAAGTATAAAAAGGAATAAGCTTATTGATTTTGAATTTCCTTCTTGTATGAAAATGATAGATACCTAAAATTGTACGTTTTTATAAATGTGTCCTTGTCATGTCCGTGTCTTAGATTTTTAGAAAATAATATATCACCGTGTCTGTGTGGTGTCGCATCTGTGTCTCACGTTCGTATTTGTACTTCTTAGGCAGAGACTGAACCTTCGACCTTAAGGAAAGTACCAAGTACCTTATCCACTAAGTATAGTAGTAGGTTAGGTGTTGGGTTTTTAAATGTATTTAGGTTCTTATTAAATAATTAATAAAATATTAATGGTAAAATGCTTTTTATGAAGTTTATAAACTAAAATATAACCTTTGAAATTTCAGGACTAAAACATTTCATTTAAAAATTCAAGAAACAGACATAATAAGATTTAAACTTTGAGTTTTCTTCAAAGTATAACTCTTACGATAGACTCTCTTCCGAAAATCAGAATCGCAATTAATTCATCTCTAACATTTTTTTCTTGATCAATGTGTTTAGATTCTACGGTGCGGTCCGCCGCCGATGAACAAGGCCATGGCTGCCCACCTTGAGGCTCTTGGATATACGCCTGAAATGCAGTTTCAGTTCTAA

mRNA sequence

ATGGAGATCTTACACACGCTCGACTTTGAGGTTCTTGTCAGTTTGGGAGTGGTCTTGGCTACCTTTGGTCTTGGTGCCGTCTTCATCGTTTTGTCCAGAAAACGCGCATCCAGAGCCTGCTTGGATCCAGAGAAGTTTAAAGAGTTTAAGCTTGTTAAGCGTACGAAGCTGAGCCATAATGTGGCGAAGTTCACTTTTATACTTCCCCGTCCCAATTCAATTCTTGGTCTTCCTATTGGACAACATATAAGTTGCAGGGGAAAGGATAGCCAGGGTGAAGAGGTTATTAAACCTTACACGCCAACTACTTTGGACTCTGATGTTGGATATTTTGAACTAGTCATAAAGATGTATCCCCAAGGCAGGATGTCACATCATTTTCGAGAAATGCGTATCGGGGACCATCTTGCTGTGAAGGGACCAAAGGGACGTTTTAAGTATCAACCTGGTCAAGTTAGAGCATTTGGGATGCTTGCTGGAGGCTCTGGCATTACACCTATGTACCAAGTTGCTCGTGCTATATTAGAAAACCCGAAAGACGAGACTAAGGTTCACCTTATTTACGCCAATGTTACATACGAAGATATTCTTTTGAAGGAGGAATTGGATATTCTTGCCACTAGATACCCTGATCGCTTCAAGATCTACTATGTGTTGAACCAGCCTCCTGAAGTTTGGGATGGTGGTGTTGGCTTTGTGTCCAAGGAAATGATTCAAGCGCATTGCCCTGCACCTGCTCCTGGCATTCAGATTCTACGGTGCGGTCCGCCGCCGATGAACAAGGCCATGGCTGCCCACCTTGAGGCTCTTGGATATACGCCTGAAATGCAGTTTCAGTTCTAA

Coding sequence (CDS)

ATGGAGATCTTACACACGCTCGACTTTGAGGTTCTTGTCAGTTTGGGAGTGGTCTTGGCTACCTTTGGTCTTGGTGCCGTCTTCATCGTTTTGTCCAGAAAACGCGCATCCAGAGCCTGCTTGGATCCAGAGAAGTTTAAAGAGTTTAAGCTTGTTAAGCGTACGAAGCTGAGCCATAATGTGGCGAAGTTCACTTTTATACTTCCCCGTCCCAATTCAATTCTTGGTCTTCCTATTGGACAACATATAAGTTGCAGGGGAAAGGATAGCCAGGGTGAAGAGGTTATTAAACCTTACACGCCAACTACTTTGGACTCTGATGTTGGATATTTTGAACTAGTCATAAAGATGTATCCCCAAGGCAGGATGTCACATCATTTTCGAGAAATGCGTATCGGGGACCATCTTGCTGTGAAGGGACCAAAGGGACGTTTTAAGTATCAACCTGGTCAAGTTAGAGCATTTGGGATGCTTGCTGGAGGCTCTGGCATTACACCTATGTACCAAGTTGCTCGTGCTATATTAGAAAACCCGAAAGACGAGACTAAGGTTCACCTTATTTACGCCAATGTTACATACGAAGATATTCTTTTGAAGGAGGAATTGGATATTCTTGCCACTAGATACCCTGATCGCTTCAAGATCTACTATGTGTTGAACCAGCCTCCTGAAGTTTGGGATGGTGGTGTTGGCTTTGTGTCCAAGGAAATGATTCAAGCGCATTGCCCTGCACCTGCTCCTGGCATTCAGATTCTACGGTGCGGTCCGCCGCCGATGAACAAGGCCATGGCTGCCCACCTTGAGGCTCTTGGATATACGCCTGAAATGCAGTTTCAGTTCTAA

Protein sequence

MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHNVAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQGRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKDETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQAHCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF

Homology

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BLAST of Moc05g27540 vs. NCBI nr
Match: XP_022152062.1 (NADH--cytochrome b5 reductase 1-like [Momordica charantia])

HSP 1 Score: 572.8 bits (1475), Expect = 1.6e-159
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN
Sbjct: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD
Sbjct: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA
Sbjct: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF
Sbjct: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 280

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BLAST of Moc05g27540 vs. NCBI nr
Match: XP_023540168.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 495.7 bits (1275), Expect = 2.6e-136
Identity = 240/280 (85.71%), Postives = 254/280 (90.71%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           MEIL  LD +VL+ L   L     GA F+V SRK ASRACLDPE FKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLIGLATALVAIVAGAFFLVSSRKSASRACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILP+ +SILGLPIGQH+SCRGKDSQGEEVIK YTP TLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMR+GD LAVKGPKG FKYQPGQVRAFGMLAGGSGITPMYQVAR+ILENP D
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGQVRAFGMLAGGSGITPMYQVARSILENPND 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           +TK+HLIYANVT EDILLKEELD+LA RYPDRFKIYYVLNQPP+VWDGGVGFVSKEMI+A
Sbjct: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPA  IQILRCGPPPMNKAMAAHL+ALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

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BLAST of Moc05g27540 vs. NCBI nr
Match: XP_038903314.1 (NADH--cytochrome b5 reductase 1-like [Benincasa hispida])

HSP 1 Score: 495.7 bits (1275), Expect = 2.6e-136
Identity = 238/280 (85.00%), Postives = 258/280 (92.14%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           ME+L  LD +VL+ L   L    +GAVF+  SRK ASRACLDPEKFKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLAAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILP+P+SILGLPIGQH+SCRGKDSQGEEVIK YTPTTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKPDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMRIGD LAVKGPKGRFKY+PGQVRAFGMLAGGSGITPMYQV+RAILENP D
Sbjct: 121 GRMSHHFREMRIGDVLAVKGPKGRFKYEPGQVRAFGMLAGGSGITPMYQVSRAILENPND 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           +TK++LIYANVT EDILLK+ELD+LA RYPDRFK+YYVLNQPPEVWDGGVGFVSKEMI+A
Sbjct: 181 KTKLYLIYANVTLEDILLKDELDLLAKRYPDRFKVYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPA  I+ILRCGPPPMNKAMA HL+ALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMADHLDALGYSPEMQFQF 280

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BLAST of Moc05g27540 vs. NCBI nr
Match: XP_008443189.1 (PREDICTED: NADH--cytochrome b5 reductase 1-like isoform X1 [Cucumis melo] >XP_008443190.1 PREDICTED: NADH--cytochrome b5 reductase 1-like isoform X2 [Cucumis melo] >ADN33982.1 NADH:cytochrome b5 reductase [Cucumis melo subsp. melo] >TYK25477.1 NADH--cytochrome b5 reductase 1-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 494.2 bits (1271), Expect = 7.4e-136
Identity = 239/280 (85.36%), Postives = 256/280 (91.43%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           ME+L  LD +VL+ L   L    +GAVF+  SRK ASRACLDPEKFKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILP+ +SILGLPIGQH+SCRGKDSQGEEVIK YTPTTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMR+GD LAVKGPKGRFKYQPGQV AFGMLAGGSGITPMYQV+RAILENP+D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           +TKVHLIYANVT EDILLKEELD+LA RYPDRFKIYYVLNQPPEVWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPA  I+ILRCGPPPMNKAM  HL+ALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of Moc05g27540 vs. NCBI nr
Match: XP_004136613.1 (NADH--cytochrome b5 reductase 1 isoform X1 [Cucumis sativus] >KGN59392.1 hypothetical protein Csa_001811 [Cucumis sativus])

HSP 1 Score: 493.4 bits (1269), Expect = 1.3e-135
Identity = 238/280 (85.00%), Postives = 255/280 (91.07%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           ME+L  LD +VL+ L   L    +GAVF+  SRK ASRACLDPEKFKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLAAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILP+ +SILGLPIGQH+SCRGKDSQGEEVIK YTPTTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMR+G+ LAVKGPKGRFKYQPGQV AFGMLAGGSGITPMYQV+RAILENP D
Sbjct: 121 GRMSHHFREMRLGESLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPDD 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           +TKVHLIYANVT EDILLKEELD+LA RYPDRFKIYYVLNQPPEVWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPA  I+ILRCGPPPMNKAM  HL+ALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

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BLAST of Moc05g27540 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 8.3e-130
Identity = 224/279 (80.29%), Postives = 244/279 (87.46%), Query Frame = 0


Query: 2   EILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHNV 61
           E L TLD ++L+ + V     G GA + + S K+  R CLDPE FKEFKLVKR +LSHNV
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKK-RRVCLDPENFKEFKLVKRHQLSHNV 63

Query: 62  AKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQG 121
           AKF F LP   S+LGLPIGQHISCRGKD QGE+VIKPYTPTTLDSDVG FELVIKMYPQG
Sbjct: 64  AKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQG 123

Query: 122 RMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKDE 181
           RMSHHFREMR+GDHLAVKGPKGRFKYQPGQ RAFGMLAGGSGITPM+QVARAILENP D+
Sbjct: 124 RMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTDK 183

Query: 182 TKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQAH 241
           TKVHLIYANVTY+DILLKEEL+ L T YP++FKI+YVLNQPPEVWDGGVGFVSKEMIQ H
Sbjct: 184 TKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQTH 243

Query: 242 CPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           CPAPA  IQILRCGPPPMNKAMAA+LEALGY+PEMQFQF
Sbjct: 244 CPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of Moc05g27540 vs. ExPASy Swiss-Prot
Match: Q54NC1 (NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 8.7e-71
Identity = 134/282 (47.52%), Postives = 188/282 (66.67%), Query Frame = 0


Query: 11  VLVSLGVVLATFGLGAVFIVLS------------RKRASRACLDPEKFKEFKLVKRTKLS 70
           +LV +G V    G+  VF + S            + +     LDP+++++F+L ++  ++
Sbjct: 7   ILVIIGSVALAAGVKYVFTLTSGSNKDKKGGEAEKGKQVEKALDPQEYRKFQLKEKFIVN 66

Query: 71  HNVAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMY 130
           HN   F F LP  + ILGLPIGQHIS R     G+EV +PYTP + D + GYF+L+IK+Y
Sbjct: 67  HNTRIFRFALPNEDDILGLPIGQHISLRAVVG-GKEVYRPYTPISSDEERGYFDLLIKVY 126

Query: 131 PQGRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENP 190
            +G MS +   M IGD + VKGPKG+F YQP   ++ GMLAGG+GITPM QV +AIL+NP
Sbjct: 127 EKGAMSGYVDNMFIGDSIEVKGPKGKFNYQPNMRKSIGMLAGGTGITPMLQVIKAILKNP 186

Query: 191 KDETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMI 250
            D+T++ L++ N+T EDILLK+ELD LA ++P +FK+YYVLN PP+ W  GVGFVSKE+I
Sbjct: 187 SDKTEISLVFGNITEEDILLKKELDELAEKHP-QFKVYYVLNNPPKGWTQGVGFVSKEII 246

Query: 251 QAHCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           ++  P+P+    ++ CGPP MNKAM  HLE +G+     F F
Sbjct: 247 ESRLPSPSDQTMVIMCGPPMMNKAMTGHLETIGFNESNIFTF 286

[Close]

BLAST of Moc05g27540 vs. ExPASy Swiss-Prot
Match: Q6BUX2 (NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.5e-70
Identity = 130/264 (49.24%), Postives = 177/264 (67.05%), Query Frame = 0


Query: 9   FEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHNVAKFTFIL 68
           F +  ++  ++++  +   F  LSRK A    L P  F++F L+++T++SHN   + F L
Sbjct: 9   FIIFATVAAIISS-AVAYYFFQLSRKNA--PVLKPNDFQKFPLIEKTRVSHNTCVYRFGL 68

Query: 69  PRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQGRMSHHFR 128
           PR    LGLPIGQHI+  G     +EV++ YTP + D ++GYF+L+IK Y  G +S H  
Sbjct: 69  PRSTDRLGLPIGQHIAI-GATINDKEVVRSYTPISTDDELGYFDLLIKAYENGNISRHVE 128

Query: 129 EMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKDETKVHLIY 188
             +IG+ + ++GPKG F Y PG V +FGM+AGG+GITPMYQ+  AIL NP+D+TKV L+Y
Sbjct: 129 SKKIGETIDIRGPKGFFTYTPGMVESFGMIAGGTGITPMYQILTAILRNPEDKTKVSLVY 188

Query: 189 ANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQAHCPAPAPG 248
           ANVT +DILLKEEL+ +A  +PDRFKIYYVLN PPE W GGVGFV+ E++  H P  +  
Sbjct: 189 ANVTEDDILLKEELNKMAREHPDRFKIYYVLNTPPENWTGGVGFVTPEIMDKHLPKASEA 248

Query: 249 IQILRCGPPPMNKAMAAHLEALGY 273
             +L CGPPPM  AM      LGY
Sbjct: 249 TNLLLCGPPPMISAMKKAAVGLGY 268

[Close]

BLAST of Moc05g27540 vs. ExPASy Swiss-Prot
Match: Q4PGW7 (NADH-cytochrome b5 reductase 1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.9e-70
Identity = 131/267 (49.06%), Postives = 185/267 (69.29%), Query Frame = 0


Query: 6   TLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHNVAKFT 65
           T   E+ + L  V+   G   V +    ++  +  L+P ++++++L+++ KLS N A + 
Sbjct: 44  TNPMELNIVLAFVVGLIGSVVVLLYFDSQKI-KPVLNPTQWQQYRLMEKQKLSDNTALYR 103

Query: 66  FILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQGRMSH 125
           F LPR N+ILGLPIGQHIS +  +  G+ V++ YTPT+ D D G+F+LV+K Y QG +S 
Sbjct: 104 FKLPRSNNILGLPIGQHISVQA-NMGGKTVVRSYTPTSSDDDHGFFDLVVKSYEQGNVSK 163

Query: 126 HFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKDETKVH 185
           +   M+IGD L+VKGPKG+ +Y PG  R  GM+AGG+G+TP  Q+ RA L+NP D+T++ 
Sbjct: 164 YIGSMKIGDLLSVKGPKGQMRYAPGLSRHIGMIAGGTGLTPCLQIIRAALKNPADKTQID 223

Query: 186 LIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQAHCPAP 245
            IYANV   DILLK+ELD LA ++ D+F+I Y LN+ PE W GGVGFV+KE ++ + P P
Sbjct: 224 FIYANVKETDILLKDELDELALKHKDQFRISYFLNEAPEGWKGGVGFVTKEALEKNLPKP 283

Query: 246 APGIQILRCGPPPMNKAMAAHLEALGY 273
           A  I++L CGPPPM KAM  HLEALGY
Sbjct: 284 ANDIKVLMCGPPPMIKAMTGHLEALGY 308

[Close]

BLAST of Moc05g27540 vs. ExPASy Swiss-Prot
Match: A1DHW1 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 262.7 bits (670), Expect = 4.8e-69
Identity = 129/242 (53.31%), Postives = 167/242 (69.01%), Query Frame = 0


Query: 38  RACLDPEKFKEFKLVKRTKLSHNVAKFTFILPRPNSILGLPIGQHISCRGK-DSQGEEVI 97
           R  L+P +F+EF L ++T +SHNV  + F LPRP  ILGLPIGQHIS     + Q +EV+
Sbjct: 54  RKVLNPTEFQEFVLKEKTDISHNVCIYRFALPRPADILGLPIGQHISLAATIEGQPKEVV 113

Query: 98  KPYTPTTLDSDVGYFELVIKMYPQGRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFG 157
           + YTP + D++ GYF+L++K YPQG +S +   ++IGD + V+GPKG   Y P   R  G
Sbjct: 114 RSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIGDTMKVRGPKGAMVYTPNMCRHIG 173

Query: 158 MLAGGSGITPMYQVARAILEN-----PKDETKVHLIYANVTYEDILLKEELDILATRYPD 217
           M+AGG+GITPM Q+ +A++ N       D TK+ LI+ANV  +DILLKEELD LA   PD
Sbjct: 174 MIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIFANVNPDDILLKEELDKLAAEDPD 233

Query: 218 RFKIYYVLNQPPEVWDGGVGFVSKEMIQAHCPAPAPGIQILRCGPPPMNKAMAAHLEALG 274
            F IYYVLN PP+ W GGVGFV+ EMI+ H PAPA  ++IL CGPPPM  AM    E+LG
Sbjct: 234 -FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASDVKILLCGPPPMISAMKKATESLG 293

[Close]

BLAST of Moc05g27540 vs. ExPASy TrEMBL
Match: A0A6J1DF63 (NADH-cytochrome b5 reductase OS=Momordica charantia OX=3673 GN=LOC111019868 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.0e-160
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN
Sbjct: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD
Sbjct: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA
Sbjct: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF
Sbjct: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 280

[Close]

BLAST of Moc05g27540 vs. ExPASy TrEMBL
Match: A0A1S3B6Z6 (NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103486847 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.6e-136
Identity = 239/280 (85.36%), Postives = 256/280 (91.43%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           ME+L  LD +VL+ L   L    +GAVF+  SRK ASRACLDPEKFKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILP+ +SILGLPIGQH+SCRGKDSQGEEVIK YTPTTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMR+GD LAVKGPKGRFKYQPGQV AFGMLAGGSGITPMYQV+RAILENP+D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           +TKVHLIYANVT EDILLKEELD+LA RYPDRFKIYYVLNQPPEVWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPA  I+ILRCGPPPMNKAM  HL+ALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of Moc05g27540 vs. ExPASy TrEMBL
Match: A0A5D3DPB8 (NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006230 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.6e-136
Identity = 239/280 (85.36%), Postives = 256/280 (91.43%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           ME+L  LD +VL+ L   L    +GAVF+  SRK ASRACLDPEKFKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILP+ +SILGLPIGQH+SCRGKDSQGEEVIK YTPTTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMR+GD LAVKGPKGRFKYQPGQV AFGMLAGGSGITPMYQV+RAILENP+D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           +TKVHLIYANVT EDILLKEELD+LA RYPDRFKIYYVLNQPPEVWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPA  I+ILRCGPPPMNKAM  HL+ALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of Moc05g27540 vs. ExPASy TrEMBL
Match: E5GBY3 (NADH-cytochrome b5 reductase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.6e-136
Identity = 239/280 (85.36%), Postives = 256/280 (91.43%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           ME+L  LD +VL+ L   L    +GAVF+  SRK ASRACLDPEKFKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILP+ +SILGLPIGQH+SCRGKDSQGEEVIK YTPTTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMR+GD LAVKGPKGRFKYQPGQV AFGMLAGGSGITPMYQV+RAILENP+D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           +TKVHLIYANVT EDILLKEELD+LA RYPDRFKIYYVLNQPPEVWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPA  I+ILRCGPPPMNKAM  HL+ALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of Moc05g27540 vs. ExPASy TrEMBL
Match: A0A0A0LFC1 (NADH-cytochrome b5 reductase OS=Cucumis sativus OX=3659 GN=Csa_3G816050 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.1e-136
Identity = 238/280 (85.00%), Postives = 255/280 (91.07%), Query Frame = 0


Query: 1   MEILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHN 60
           ME+L  LD +VL+ L   L    +GAVF+  SRK ASRACLDPEKFKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLAAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQ 120
           VAKFTFILP+ +SILGLPIGQH+SCRGKDSQGEEVIK YTPTTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKD 180
           GRMSHHFREMR+G+ LAVKGPKGRFKYQPGQV AFGMLAGGSGITPMYQV+RAILENP D
Sbjct: 121 GRMSHHFREMRLGESLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPDD 180

Query: 181 ETKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQA 240
           +TKVHLIYANVT EDILLKEELD+LA RYPDRFKIYYVLNQPPEVWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           HCPAPA  I+ILRCGPPPMNKAM  HL+ALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of Moc05g27540 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 464.5 bits (1194), Expect = 5.9e-131
Identity = 224/279 (80.29%), Postives = 244/279 (87.46%), Query Frame = 0


Query: 2   EILHTLDFEVLVSLGVVLATFGLGAVFIVLSRKRASRACLDPEKFKEFKLVKRTKLSHNV 61
           E L TLD ++L+ + V     G GA + + S K+  R CLDPE FKEFKLVKR +LSHNV
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKK-RRVCLDPENFKEFKLVKRHQLSHNV 63

Query: 62  AKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGYFELVIKMYPQG 121
           AKF F LP   S+LGLPIGQHISCRGKD QGE+VIKPYTPTTLDSDVG FELVIKMYPQG
Sbjct: 64  AKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQG 123

Query: 122 RMSHHFREMRIGDHLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKDE 181
           RMSHHFREMR+GDHLAVKGPKGRFKYQPGQ RAFGMLAGGSGITPM+QVARAILENP D+
Sbjct: 124 RMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTDK 183

Query: 182 TKVHLIYANVTYEDILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQAH 241
           TKVHLIYANVTY+DILLKEEL+ L T YP++FKI+YVLNQPPEVWDGGVGFVSKEMIQ H
Sbjct: 184 TKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQTH 243

Query: 242 CPAPAPGIQILRCGPPPMNKAMAAHLEALGYTPEMQFQF 281
           CPAPA  IQILRCGPPPMNKAMAA+LEALGY+PEMQFQF
Sbjct: 244 CPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of Moc05g27540 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 211.5 bits (537), Expect = 9.0e-55
Identity = 115/279 (41.22%), Postives = 170/279 (60.93%), Query Frame = 0


Query: 21  TFGLGAVFIVLSRKRAS-RACLDPEKFKEFKLVKRTKLSHNVAKFTFILPRPNSILGLPI 80
           T G   V++  +++    +  L+P+K+ EFKL    ++SHN   F F    P++ LGL +
Sbjct: 52  TSGNNLVYLDQAKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSFD-PSAELGLHV 111

Query: 81  GQHISCR---GKDSQGEE--VIKPYTPTTLDSDVGYFELVIKMYPQGRMSHHFREMRIGD 140
              +  R   G +++G+   VI+PYTP +     GYF+L+IK+YP G+MS HF  ++ GD
Sbjct: 112 ASCLLTRAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGD 171

Query: 141 HLAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMYQVARAILENPKDETKVHLIYANVTYE 200
            L VKGP  +FKY P   +  GM+AGGSGITPM QV  AI++NP+D T++ L+YANV+ +
Sbjct: 172 VLEVKGPVEKFKYSPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPD 231

Query: 201 DILLKEELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQAHCPAPAPGIQILRC 260
           DILLK++LD+L   +P+  KI+Y ++ P + W GGVG++SK+M     P P     IL C
Sbjct: 232 DILLKQKLDVLQANHPN-LKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVC 291

Query: 261 GPPPMNKAMAAH-------------LEALGYTPEMQFQF 281
           GPP M + ++               L+ LGYT EM F+F
Sbjct: 292 GPPGMMEHISGGKAPDWSQGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of Moc05g27540 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 185.7 bits (470), Expect = 5.3e-47
Identity = 104/261 (39.85%), Postives = 157/261 (60.15%), Query Frame = 0


Query: 39  ACLDPEKFKEFKLVKRTKLSHNVAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKP 98
           A ++P      +LV++T +SH+V KF F LP  + +LGLP+G+HI      +  +  ++ 
Sbjct: 655 ALVNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND-KLCLRA 714

Query: 99  YTPTTLDSDVGYFELVIKMY-----PQ----GRMSHHFREMRIGDHLAVKGPKGRFKY-- 158
           YTP++    VGYFELV+K+Y     P+    G MS +   + IG  L +KGP G  +Y  
Sbjct: 715 YTPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLG 774

Query: 159 --------QPGQVRAFGMLAGGSGITPMYQVARAILENPKDETKVHLIYANVTYEDILLK 218
                   +P       MLAGG+GITP+YQ+ +AIL++P+DET++++IYAN T EDILL+
Sbjct: 775 KGSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLR 834

Query: 219 EELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQAHCPAPAPGIQI-LRCGPPP 278
           EELD  A +YPDR K++YV+    E W    GF+S+ +++ H P    G  + + CGPPP
Sbjct: 835 EELDGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPP 894

[Close]

BLAST of Moc05g27540 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 176.4 bits (446), Expect = 3.2e-44
Identity = 93/255 (36.47%), Postives = 155/255 (60.78%), Query Frame = 0


Query: 39  ACLDPEKFKEFKLVKRTKLSHNVAKFTFILPRPNSILGLPIGQHISCRGKDSQGEEVIKP 98
           A ++P +    +L+++T +SH+V KF F LP  +  LGLP+G+H+     +   +  ++ 
Sbjct: 655 ALVNPREKIPVRLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCA-NINDKLCLRA 714

Query: 99  YTPTTLDSDVGYFELVIKMY-----PQ----GRMSHHFREMRIGDHLAVKGPKGRFKY-- 158
           YTPT+    VG+ +LV+K+Y     P+    G MS H   + IG  + +KGP G  +Y  
Sbjct: 715 YTPTSAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKG 774

Query: 159 --------QPGQVRAFGMLAGGSGITPMYQVARAILENPKDETKVHLIYANVTYEDILLK 218
                   +P   +   MLAGG+GITP+YQ+ ++IL +P+DET+++++YAN T +DIL++
Sbjct: 775 KGNFLVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVR 834

Query: 219 EELDILATRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIQAHCPAPAPGIQI-LRCGPPP 273
           EEL+  A+++ +R KI+YV+    E W    GF+++ +++ H P    G  + L CGPPP
Sbjct: 835 EELEGWASKHKERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPP 894

The following BLAST results are available for this feature:

BLAST of Moc05g27540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152062.1	1.6e-159	100.00	NADH--cytochrome b5 reductase 1-like [Momordica charantia]	[more]
XP_023540168.1	2.6e-136	85.71	NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_038903314.1	2.6e-136	85.00	NADH--cytochrome b5 reductase 1-like [Benincasa hispida]	[more]
XP_008443189.1	7.4e-136	85.36	PREDICTED: NADH--cytochrome b5 reductase 1-like isoform X1 [Cucumis melo] >XP_00...	[more]
XP_004136613.1	1.3e-135	85.00	NADH--cytochrome b5 reductase 1 isoform X1 [Cucumis sativus] >KGN59392.1 hypothe...	[more]

BLAST of Moc05g27540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNT1	8.3e-130	80.29	NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=...	[more]
Q54NC1	8.7e-71	47.52	NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE...	[more]
Q6BUX2	1.5e-70	49.24	NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS...	[more]
Q4PGW7	1.9e-70	49.06	NADH-cytochrome b5 reductase 1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=23...	[more]
A1DHW1	4.8e-69	53.31	NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3...	[more]

BLAST of Moc05g27540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DF63	8.0e-160	100.00	NADH-cytochrome b5 reductase OS=Momordica charantia OX=3673 GN=LOC111019868 PE=3...	[more]
A0A1S3B6Z6	3.6e-136	85.36	NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103486847 PE=3 SV=1	[more]
A0A5D3DPB8	3.6e-136	85.36	NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
E5GBY3	3.6e-136	85.36	NADH-cytochrome b5 reductase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A0A0LFC1	6.1e-136	85.00	NADH-cytochrome b5 reductase OS=Cucumis sativus OX=3659 GN=Csa_3G816050 PE=3 SV=...	[more]

BLAST of Moc05g27540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17770.1	5.9e-131	80.29	NADH:cytochrome B5 reductase 1	[more]
AT5G20080.1	9.0e-55	41.22	FAD/NAD(P)-binding oxidoreductase	[more]
AT1G37130.1	5.3e-47	39.85	nitrate reductase 2	[more]
AT1G77760.1	3.2e-44	36.47	nitrate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 97..104 score: 44.79 coord: 133..147 score: 48.06 coord: 194..205 score: 64.06 coord: 250..258 score: 59.49 coord: 156..175 score: 66.67 coord: 75..86 score: 57.47
IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	PRINTS	PR00371	FPNCR	coord: 156..175 score: 37.99 coord: 250..258 score: 37.16 coord: 194..205 score: 43.58
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 49..147 e-value: 5.1E-32 score: 110.2
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 157..263 e-value: 4.1E-30 score: 104.6
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 36..137 e-value: 8.1E-35 score: 121.3
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..280
None	No IPR available	PANTHER	PTHR19370:SF203	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..280
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 49..280 e-value: 6.04327E-116 score: 330.683
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 138..280 e-value: 2.8E-43 score: 149.1
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 136..276
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 44..148 score: 14.267059
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 41..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g27540.1	Moc05g27540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0016491	oxidoreductase activity