Moc05g27060 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc05g27060 vs. NCBI nr
Match: XP_022155139.1 (uncharacterized protein LOC111022280 [Momordica charantia])

HSP 1 Score: 793.9 bits (2049), Expect = 1.1e-225
Identity = 440/613 (71.78%), Postives = 468/613 (76.35%), Query Frame = 0

Query: 126 PGAPGEKGASSMQPGDREPIPNDGGVDYSLRDNDLRKYLTQKKKRASREPEDSPSYSREF 185
           PGAPGEKGA S+QPG+REPIPND GVDYSLRDNDLRK+LT KKK+AS EPEDS SYSREF
Sbjct: 5   PGAPGEKGAPSIQPGNREPIPNDEGVDYSLRDNDLRKHLTDKKKKASWEPEDSLSYSREF 64

Query: 186 SNSNLKAQSKYKPLAPEAVITREEFDLMKHKFDEQVEALKARCEKKDCSFDNGDLGESPF 245
           SNSNLKAQSKYKPL PEAVI REEFDLMKH+FDEQVEALKARCEKK+  FD+ DLGESPF
Sbjct: 65  SNSNLKAQSKYKPLIPEAVINREEFDLMKHRFDEQVEALKARCEKKESPFDDDDLGESPF 124

Query: 246 TADILEAPIPPKFKTPAMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCRVFQIALTGSA 305
           T+DI+EAPIPPKFKTP MKPYDGSKDPKDYVEVFEGLMDFQAATDAIKC  FQIALTGSA
Sbjct: 125 TSDIMEAPIPPKFKTPTMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCLAFQIALTGSA 184

Query: 306 RL-------------------------------KTATHLATIRQREGETLREYVTRFQEE 365
           RL                               KTATHLATIRQ+E ETL   +      
Sbjct: 185 RLWCRRLPARSISTYSQLRKEFIGQFSFRHYDRKTATHLATIRQKEDETLTVKLG----- 244

Query: 366 QLKEAPATFAEVLQKAKKVIDGQELLRTKTGQPEKQVVQRKLSQDKRRTDVSRPYERYTP 425
             +EAPATFAEVLQ AKKVIDGQELLRTKT +PEKQ+ Q++LSQ KR+ D          
Sbjct: 245 --EEAPATFAEVLQNAKKVIDGQELLRTKTDRPEKQIDQKRLSQKKRKDD---------- 304

Query: 426 TTIPVSEILTNIEDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHRDHGHNTSSCWELKRQI 485
                    +  +D G      R E  R +        Y R           CWELKRQI
Sbjct: 305 ---------SKSKDKGSSSSGSRTEYRRSESGPSRSRPYER-----------CWELKRQI 364

Query: 486 EDLIQDGYFKKFVGKPRSNSVEKMEERKRSRTPSRREDRPAVINTIFGGPSGGQSRNKRN 545
           EDLIQD YFKKFVGKPRSNSVEK EERKRSRTP RREDRPAVINTIFGGPSGGQ  NKR 
Sbjct: 365 EDLIQDSYFKKFVGKPRSNSVEKKEERKRSRTPPRREDRPAVINTIFGGPSGGQFENKRK 424

Query: 546 ELARAARRKVCIIREQRPTFPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGA 605
           ELA  ARRKV IIREQ+PT  ITF   DLE VHLPHNDALVIAPLIDHV+VRRVLVDGGA
Sbjct: 425 ELACEARRKVSIIREQKPTCSITFKDTDLEGVHLPHNDALVIAPLIDHVLVRRVLVDGGA 484

Query: 606 SANILSLLTYLTLGWTRSQLKKSLTPLVGFSGESITPEGCIDLPVTFGQDKTQVTKMAEF 665
           SANILSL TYL L  TRSQLKKS TPLVGFS ES++PEGCIDLPVT GQD TQVT+MAEF
Sbjct: 485 SANILSLPTYLALRGTRSQLKKSPTPLVGFSAESVSPEGCIDLPVTIGQDSTQVTQMAEF 544

Query: 666 VVIDGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTPHGVGIVRGEQTASRECYASALKG 708
           VVIDGR AYNAIF RPIIHSF+AVPS LHQVLKYSTP+GVG VRGEQ  SRECYASALK 
Sbjct: 545 VVIDGRLAYNAIFERPIIHSFQAVPSILHQVLKYSTPNGVGTVRGEQKTSRECYASALKR 580

BLAST of Moc05g27060 vs. NCBI nr
Match: XP_022152854.1 (uncharacterized protein LOC111020479 [Momordica charantia])

HSP 1 Score: 750.4 bits (1936), Expect = 1.5e-212
Identity = 416/601 (69.22%), Postives = 444/601 (73.88%), Query Frame = 0

Query: 191 KAQSKYKPLAPEAVITREEFDLMKHKFDEQVEALKARCEKKDCSFDNGDLGESPFTADIL 250
           KA+S Y P+ P  VITREEFD +K KFD QVEALKARCEKK+ SFD+GDLGE  F++DIL
Sbjct: 64  KAESSYNPITP-GVITREEFDQLKSKFDAQVEALKARCEKKESSFDDGDLGELSFSSDIL 123

Query: 251 EAPIPPKFKTPAMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCRVFQIALTGSARL--- 310
           EA IPPKFKTP MKPYDGSKDPKDYVEVFE LMDFQAATDAIKC  FQIALTGSARL   
Sbjct: 124 EALIPPKFKTPTMKPYDGSKDPKDYVEVFESLMDFQAATDAIKCCAFQIALTGSARLWYR 183

Query: 311 ----------------------------KTATHLATIRQREGETLREYVTRFQEEQLK-- 370
                                       KT THLATIRQ+EGETLREYVTRF EEQLK  
Sbjct: 184 RLPARLISTYSQLRKEFISQFSSRHYDRKTPTHLATIRQKEGETLREYVTRFPEEQLKVA 243

Query: 371 --------------------------EAPATFAEVLQKAKKVIDGQELLRTKTGQPEKQV 430
                                     EAPATFAEVLQK KKVIDGQELLRTKTG+PEK +
Sbjct: 244 HCSDDSAMCYFLTGLADETLTVKLREEAPATFAEVLQKTKKVIDGQELLRTKTGRPEKNI 303

Query: 431 VQRKLSQDKRRTD-------------------------VSRPYERYTPTTIPVSEILTNI 490
            Q +  +DK + D                          SRPYE YTPTTIP+ EILTNI
Sbjct: 304 DQGRAGKDKGKADSKSRDKGPSSSSSRVDYRRSNSSHNQSRPYEHYTPTTIPIFEILTNI 363

Query: 491 EDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHRDHGHNTSSCWELKRQIEDLIQDGYFKKF 550
           E++GMEKLLKRPEKLRGDPEKRN DKYCRFHRDHGHNTS+ WELKRQIEDLIQDGYFKKF
Sbjct: 364 EETGMEKLLKRPEKLRGDPEKRNTDKYCRFHRDHGHNTSNYWELKRQIEDLIQDGYFKKF 423

Query: 551 VGKPRSNSVEKMEERKRSRTPSRREDRPAVINTIFGGPSGGQSRNKRNELARAARRKVCI 610
           VGKPRSNSVEK EERKR RTP RR+DRPAVI             NK+ ELAR ARR+VCI
Sbjct: 424 VGKPRSNSVEKKEERKRLRTPPRRDDRPAVI-------------NKKKELAREARREVCI 483

Query: 611 IREQRPTFPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLLTYLT 670
           IREQRPT  I F+ ADLE VHLPHNDALVIAPLID V+VRR+LVDGGASANILSL TYL 
Sbjct: 484 IREQRPTSSIAFNHADLEGVHLPHNDALVIAPLIDLVLVRRILVDGGASANILSLSTYLA 543

Query: 671 LGWTRSQLKKSLTPLVGFSGESITPEGCIDLPVTFGQDKTQVTKMAEFVVIDGRSAYNAI 708
           LGWTRSQLKKS TPLVGFSGESI+ EGCIDLPV+  QD TQVT+MAEFVVIDGRSAYNAI
Sbjct: 544 LGWTRSQLKKSPTPLVGFSGESISLEGCIDLPVSIRQDDTQVTQMAEFVVIDGRSAYNAI 603

BLAST of Moc05g27060 vs. NCBI nr
Match: XP_022152854.1 (uncharacterized protein LOC111020479 [Momordica charantia])

HSP 1 Score: 47.0 bits (110), Expect = 7.9e-01
Identity = 45/149 (30.20%), Postives = 66/149 (44.30%), Query Frame = 0

Query: 1   MVHPANSANTTEQRGVNADNGPQRDLGARIVEDQVRAGQEGDLPRRFARHANQELPLAHP 60
           MV PANS NT ++R + A++G QR++GA +VE Q       +   R AR     LP AHP
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEPLCRSARITTPVLPPAHP 60

Query: 61  KPSKANRGRGGTSRKTSQRANQEADPEALSTLQRELDDMCHRLRTMEEMYADATRANRTA 120
           KPSKA       +  T     +E   +  S    +++ +  R    E  + D      + 
Sbjct: 61  KPSKA---ESSYNPITPGVITREEFDQLKSKFDAQVEALKARCEKKESSFDDGDLGELSF 120

Query: 121 SPSIAPG-APGEKGASSMQPGDREPIPND 149
           S  I     P +    +M+P D    P D
Sbjct: 121 SSDILEALIPPKFKTPTMKPYDGSKDPKD 146

HSP 2 Score: 747.3 bits (1928), Expect = 1.2e-211
Identity = 399/551 (72.41%), Postives = 446/551 (80.94%), Query Frame = 0

Query: 187 NSNLKAQSKYKPLAPEAVITREEFDLMKHKFDEQVEALKARCEKKDCSFDNGDLGESPFT 246
           +SN +A+S + P  P+ VITREEFD ++ K + QVEALKA+CE+K+   ++GDLGESPFT
Sbjct: 2   SSNQQAESSHNPATPDGVITREEFDQLRGKLNAQVEALKAKCEQKEGPLNDGDLGESPFT 61

Query: 247 ADILEAPIPPKFKTPAMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCRVFQIALTGSAR 306
           +D+LEA        P +K YDGSKDPKDYVEVFEGLMDFQAA+DAIKCR FQIALTGSAR
Sbjct: 62  SDVLEA--------PTVKSYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR 121

Query: 307 L-KTATHLATIRQREGETLREYVTRFQEEQL-----KEAPATFAEVLQKAKKVIDGQELL 366
           L      L   +  +   +  ++T   +E L     KEAPATFAEVLQKAKKVIDGQELL
Sbjct: 122 LWFQEDQLKVAQSSDDSAMCYFLTGLADEALTVKLGKEAPATFAEVLQKAKKVIDGQELL 181

Query: 367 RTKTGQPEKQVVQRKLSQDKR------------------RTDV-----SRPYERYTPTTI 426
           RTKTG+PE+ + + +  +D++                  R  V     SRPYER+TPTTI
Sbjct: 182 RTKTGRPERGIDRGRSGKDEKADLKSKDKGSFSSGRAEFRRAVNGPTRSRPYERFTPTTI 241

Query: 427 PVSEILTNIEDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHRDHGHNTSSCWELKRQIEDL 486
           P+SEILTNIE+SGMEKLLKRPEKLRG PE+RNKDKYCRFHR+H HNTS  WELKRQIEDL
Sbjct: 242 PISEILTNIEESGMEKLLKRPEKLRGAPERRNKDKYCRFHREHDHNTSDRWELKRQIEDL 301

Query: 487 IQDGYFKKFVGKPRSNSVEKMEERKRSRTPSRREDRPAVINTIFGGPSGGQSRNKRNELA 546
           IQD YFKKFVGKPR++S EK EERK SRTP RR DRPAVINTIFGGPSGGQS +KR ELA
Sbjct: 302 IQDDYFKKFVGKPRTSSAEKKEERKLSRTPLRRIDRPAVINTIFGGPSGGQSGHKRKELA 361

Query: 547 RAARRKVCIIREQRPTFPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASAN 606
           RAARR+VCIIREQRPT PITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVD G SAN
Sbjct: 362 RAARREVCIIREQRPTCPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDEGVSAN 421

Query: 607 ILSLLTYLTLGWTRSQLKKSLTPLVGFSGESITPEGCIDLPVTFGQDKTQVTKMAEFVVI 666
           I+SLLTYL LGWTRSQLKKS TPLVGFS ES+ PEGCIDLPVT G D+TQVT+MAEFVVI
Sbjct: 422 IVSLLTYLALGWTRSQLKKSTTPLVGFSRESVIPEGCIDLPVTLGHDQTQVTQMAEFVVI 481

Query: 667 DGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTPHGVGIVRGEQTASRECYASALKGSSV 709
           DGRSAYNAIFGRPIIHSFRA+PSTLHQVLKYSTP+GVG+VRGEQ ASRECYASALKGSSV
Sbjct: 482 DGRSAYNAIFGRPIIHSFRAIPSTLHQVLKYSTPNGVGMVRGEQIASRECYASALKGSSV 541

BLAST of Moc05g27060 vs. NCBI nr
Match: XP_022137317.1 (uncharacterized protein LOC111008813 [Momordica charantia])

HSP 1 Score: 672.9 bits (1735), Expect = 2.9e-189
Identity = 363/529 (68.62%), Postives = 399/529 (75.43%), Query Frame = 0

Query: 190 LKAQSKYKPLAPEAVITREEFDLMKHKFDEQVEALKARCEKKDCSFDNGDLGESPFTADI 249
           +KA+S   P  P  VITREEFD ++ + D QVEALKA+CE+K+   ++GDLGESPFT+D+
Sbjct: 2   VKAESSRNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDV 61

Query: 250 LEAPIPPKFKTPAMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCRVFQIALTGSARL-- 309
           LEAPIPPKFK P +KPYDGSKDPKDYVEVFE LMDFQAA+DAIKCR F+IALTGSARL  
Sbjct: 62  LEAPIPPKFKAPTVKPYDGSKDPKDYVEVFESLMDFQAASDAIKCRAFRIALTGSARLWY 121

Query: 310 -----------------------------KTATHLATIRQREGETLREYVTRFQEEQLK- 369
                                        KTATHLATIRQ+EGETLREYVTRFQEEQLK 
Sbjct: 122 RRLPAXSISTYSQLRREFLAXFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKV 181

Query: 370 ---------------------------EAPATFAEVLQKAKKVIDGQELLRTKTGQPEKQ 429
                                      EAPATFAEVLQKAKKVIDGQELLRTKTG+PE++
Sbjct: 182 AHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERK 241

Query: 430 VVQRKLSQDKRRTDV------------------------SRPYERYTPTTIPVSEILTNI 489
           + + +  +D    D                         SRPYER+TPTTIP+SEILTNI
Sbjct: 242 IGRGRSGKDIENADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNI 301

Query: 490 EDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHRDHGHNTSSCWELKRQIEDLIQDGYFKKF 549
           E+SGMEKLLKRPEKLRG PE+R+KDKYCRFHR+HGHNTS  WELKRQIE+LIQDGYFKKF
Sbjct: 302 EESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKRQIENLIQDGYFKKF 361

Query: 550 VGKPRSNSVEKMEERKRSRTPSRREDRPAVINTIFGGPSGGQSRNKRNELARAARRKVCI 609
           VGKPR++S EK EERKRSRTP RR DRPAVINTIFGGPSGGQS  KR ELARAARR+VCI
Sbjct: 362 VGKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGRKRKELARAARREVCI 421

Query: 610 IREQRPTFPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLLTYLT 636
           IREQRPT PITFD ADLEEVHLPHNDALVIAPLIDHVVV RVLVDGG SANILSL TYL 
Sbjct: 422 IREQRPTCPITFDGADLEEVHLPHNDALVIAPLIDHVVVXRVLVDGGTSANILSLPTYLA 481

BLAST of Moc05g27060 vs. NCBI nr
Match: XP_022152110.1 (uncharacterized protein LOC111019899 [Momordica charantia])

HSP 1 Score: 597.4 bits (1539), Expect = 1.6e-166
Identity = 313/390 (80.26%), Postives = 336/390 (86.15%), Query Frame = 0

Query: 336 LKEAPATFAEVLQKAKKVIDGQELLRTKTGQPEKQVVQRKL---SQDK----------RR 395
           ++EAPATFAEVLQKAKKVIDGQELLRTK GQ             S+DK          RR
Sbjct: 18  VEEAPATFAEVLQKAKKVIDGQELLRTKIGQGRSGKDMENTDPKSKDKGSFSNGRAEYRR 77

Query: 396 TD----VSRPYERYTPTTIPVSEILTNIEDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHR 455
            +     SRPYER+TPTTIP+SEILTNIE+SGMEKLLKRPEKLRG PE+R+KDKYCRFHR
Sbjct: 78  AENGPTRSRPYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHR 137

Query: 456 DHGHNTSSCWELKRQIEDLIQDGYFKKFVGKPRSNSVEKMEERKRSRTPSRREDRPAVIN 515
           +HGHNTS  WELK QIEDLIQDGYFKKFVGKPR++S EK EERKRSRTP RR DRPAVIN
Sbjct: 138 EHGHNTSDYWELKSQIEDLIQDGYFKKFVGKPRTSSAEKKEERKRSRTPPRRTDRPAVIN 197

Query: 516 TIFGGPSGGQSRNKRNELARAARRKVCIIREQRPTFPITFDSADLEEVHLPHNDALVIAP 575
           TIFGGPSGGQS +KR +LARAARR+VCIIREQRPT PITFD ADL EVHLPHNDALVIAP
Sbjct: 198 TIFGGPSGGQSGHKRKKLARAARREVCIIREQRPTCPITFDXADLXEVHLPHNDALVIAP 257

Query: 576 LIDHVVVRRVLVDGGASANILSLLTYLTLGWTRSQLKKSLTPLVGFSGESITPEGCIDLP 635
           LIDHVVVRRVLVDGGASANILSL TYL LGWTRSQLKKS TPLVGFSGES+ PEGCIDLP
Sbjct: 258 LIDHVVVRRVLVDGGASANILSLPTYLALGWTRSQLKKSPTPLVGFSGESVVPEGCIDLP 317

Query: 636 VTFGQDKTQVTKMAEFVVIDGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTPHGVGIVR 695
           VT GQD+T+VT+MAEFVV+DGRSAYNAIFGRPIIHSFRA+PSTLHQVLKYSTP+GVG VR
Sbjct: 318 VTLGQDQTRVTQMAEFVVVDGRSAYNAIFGRPIIHSFRAIPSTLHQVLKYSTPNGVGTVR 377

Query: 696 GEQTASRECYASALKGSSVCALEDQASGDG 709
           GEQTASRECYAS LKG+SVCALE   S DG
Sbjct: 378 GEQTASRECYASXLKGTSVCALETLTSRDG 407

BLAST of Moc05g27060 vs. ExPASy TrEMBL
Match: A0A6J1DPC9 (uncharacterized protein LOC111022280 OS=Momordica charantia OX=3673 GN=LOC111022280 PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 5.6e-226
Identity = 440/613 (71.78%), Postives = 468/613 (76.35%), Query Frame = 0

Query: 126 PGAPGEKGASSMQPGDREPIPNDGGVDYSLRDNDLRKYLTQKKKRASREPEDSPSYSREF 185
           PGAPGEKGA S+QPG+REPIPND GVDYSLRDNDLRK+LT KKK+AS EPEDS SYSREF
Sbjct: 5   PGAPGEKGAPSIQPGNREPIPNDEGVDYSLRDNDLRKHLTDKKKKASWEPEDSLSYSREF 64

Query: 186 SNSNLKAQSKYKPLAPEAVITREEFDLMKHKFDEQVEALKARCEKKDCSFDNGDLGESPF 245
           SNSNLKAQSKYKPL PEAVI REEFDLMKH+FDEQVEALKARCEKK+  FD+ DLGESPF
Sbjct: 65  SNSNLKAQSKYKPLIPEAVINREEFDLMKHRFDEQVEALKARCEKKESPFDDDDLGESPF 124

Query: 246 TADILEAPIPPKFKTPAMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCRVFQIALTGSA 305
           T+DI+EAPIPPKFKTP MKPYDGSKDPKDYVEVFEGLMDFQAATDAIKC  FQIALTGSA
Sbjct: 125 TSDIMEAPIPPKFKTPTMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCLAFQIALTGSA 184

Query: 306 RL-------------------------------KTATHLATIRQREGETLREYVTRFQEE 365
           RL                               KTATHLATIRQ+E ETL   +      
Sbjct: 185 RLWCRRLPARSISTYSQLRKEFIGQFSFRHYDRKTATHLATIRQKEDETLTVKLG----- 244

Query: 366 QLKEAPATFAEVLQKAKKVIDGQELLRTKTGQPEKQVVQRKLSQDKRRTDVSRPYERYTP 425
             +EAPATFAEVLQ AKKVIDGQELLRTKT +PEKQ+ Q++LSQ KR+ D          
Sbjct: 245 --EEAPATFAEVLQNAKKVIDGQELLRTKTDRPEKQIDQKRLSQKKRKDD---------- 304

Query: 426 TTIPVSEILTNIEDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHRDHGHNTSSCWELKRQI 485
                    +  +D G      R E  R +        Y R           CWELKRQI
Sbjct: 305 ---------SKSKDKGSSSSGSRTEYRRSESGPSRSRPYER-----------CWELKRQI 364

Query: 486 EDLIQDGYFKKFVGKPRSNSVEKMEERKRSRTPSRREDRPAVINTIFGGPSGGQSRNKRN 545
           EDLIQD YFKKFVGKPRSNSVEK EERKRSRTP RREDRPAVINTIFGGPSGGQ  NKR 
Sbjct: 365 EDLIQDSYFKKFVGKPRSNSVEKKEERKRSRTPPRREDRPAVINTIFGGPSGGQFENKRK 424

Query: 546 ELARAARRKVCIIREQRPTFPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGA 605
           ELA  ARRKV IIREQ+PT  ITF   DLE VHLPHNDALVIAPLIDHV+VRRVLVDGGA
Sbjct: 425 ELACEARRKVSIIREQKPTCSITFKDTDLEGVHLPHNDALVIAPLIDHVLVRRVLVDGGA 484

Query: 606 SANILSLLTYLTLGWTRSQLKKSLTPLVGFSGESITPEGCIDLPVTFGQDKTQVTKMAEF 665
           SANILSL TYL L  TRSQLKKS TPLVGFS ES++PEGCIDLPVT GQD TQVT+MAEF
Sbjct: 485 SANILSLPTYLALRGTRSQLKKSPTPLVGFSAESVSPEGCIDLPVTIGQDSTQVTQMAEF 544

Query: 666 VVIDGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTPHGVGIVRGEQTASRECYASALKG 708
           VVIDGR AYNAIF RPIIHSF+AVPS LHQVLKYSTP+GVG VRGEQ  SRECYASALK 
Sbjct: 545 VVIDGRLAYNAIFERPIIHSFQAVPSILHQVLKYSTPNGVGTVRGEQKTSRECYASALKR 580

BLAST of Moc05g27060 vs. ExPASy TrEMBL
Match: A0A6J1DHB3 (uncharacterized protein LOC111020479 OS=Momordica charantia OX=3673 GN=LOC111020479 PE=4 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 7.0e-213
Identity = 416/601 (69.22%), Postives = 444/601 (73.88%), Query Frame = 0

Query: 191 KAQSKYKPLAPEAVITREEFDLMKHKFDEQVEALKARCEKKDCSFDNGDLGESPFTADIL 250
           KA+S Y P+ P  VITREEFD +K KFD QVEALKARCEKK+ SFD+GDLGE  F++DIL
Sbjct: 64  KAESSYNPITP-GVITREEFDQLKSKFDAQVEALKARCEKKESSFDDGDLGELSFSSDIL 123

Query: 251 EAPIPPKFKTPAMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCRVFQIALTGSARL--- 310
           EA IPPKFKTP MKPYDGSKDPKDYVEVFE LMDFQAATDAIKC  FQIALTGSARL   
Sbjct: 124 EALIPPKFKTPTMKPYDGSKDPKDYVEVFESLMDFQAATDAIKCCAFQIALTGSARLWYR 183

Query: 311 ----------------------------KTATHLATIRQREGETLREYVTRFQEEQLK-- 370
                                       KT THLATIRQ+EGETLREYVTRF EEQLK  
Sbjct: 184 RLPARLISTYSQLRKEFISQFSSRHYDRKTPTHLATIRQKEGETLREYVTRFPEEQLKVA 243

Query: 371 --------------------------EAPATFAEVLQKAKKVIDGQELLRTKTGQPEKQV 430
                                     EAPATFAEVLQK KKVIDGQELLRTKTG+PEK +
Sbjct: 244 HCSDDSAMCYFLTGLADETLTVKLREEAPATFAEVLQKTKKVIDGQELLRTKTGRPEKNI 303

Query: 431 VQRKLSQDKRRTD-------------------------VSRPYERYTPTTIPVSEILTNI 490
            Q +  +DK + D                          SRPYE YTPTTIP+ EILTNI
Sbjct: 304 DQGRAGKDKGKADSKSRDKGPSSSSSRVDYRRSNSSHNQSRPYEHYTPTTIPIFEILTNI 363

Query: 491 EDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHRDHGHNTSSCWELKRQIEDLIQDGYFKKF 550
           E++GMEKLLKRPEKLRGDPEKRN DKYCRFHRDHGHNTS+ WELKRQIEDLIQDGYFKKF
Sbjct: 364 EETGMEKLLKRPEKLRGDPEKRNTDKYCRFHRDHGHNTSNYWELKRQIEDLIQDGYFKKF 423

Query: 551 VGKPRSNSVEKMEERKRSRTPSRREDRPAVINTIFGGPSGGQSRNKRNELARAARRKVCI 610
           VGKPRSNSVEK EERKR RTP RR+DRPAVI             NK+ ELAR ARR+VCI
Sbjct: 424 VGKPRSNSVEKKEERKRLRTPPRRDDRPAVI-------------NKKKELAREARREVCI 483

Query: 611 IREQRPTFPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLLTYLT 670
           IREQRPT  I F+ ADLE VHLPHNDALVIAPLID V+VRR+LVDGGASANILSL TYL 
Sbjct: 484 IREQRPTSSIAFNHADLEGVHLPHNDALVIAPLIDLVLVRRILVDGGASANILSLSTYLA 543

Query: 671 LGWTRSQLKKSLTPLVGFSGESITPEGCIDLPVTFGQDKTQVTKMAEFVVIDGRSAYNAI 708
           LGWTRSQLKKS TPLVGFSGESI+ EGCIDLPV+  QD TQVT+MAEFVVIDGRSAYNAI
Sbjct: 544 LGWTRSQLKKSPTPLVGFSGESISLEGCIDLPVSIRQDDTQVTQMAEFVVIDGRSAYNAI 603

BLAST of Moc05g27060 vs. ExPASy TrEMBL
Match: A0A6J1DHB3 (uncharacterized protein LOC111020479 OS=Momordica charantia OX=3673 GN=LOC111020479 PE=4 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 3.8e-01
Identity = 45/149 (30.20%), Postives = 66/149 (44.30%), Query Frame = 0

Query: 1   MVHPANSANTTEQRGVNADNGPQRDLGARIVEDQVRAGQEGDLPRRFARHANQELPLAHP 60
           MV PANS NT ++R + A++G QR++GA +VE Q       +   R AR     LP AHP
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEPLCRSARITTPVLPPAHP 60

Query: 61  KPSKANRGRGGTSRKTSQRANQEADPEALSTLQRELDDMCHRLRTMEEMYADATRANRTA 120
           KPSKA       +  T     +E   +  S    +++ +  R    E  + D      + 
Sbjct: 61  KPSKA---ESSYNPITPGVITREEFDQLKSKFDAQVEALKARCEKKESSFDDGDLGELSF 120

Query: 121 SPSIAPG-APGEKGASSMQPGDREPIPND 149
           S  I     P +    +M+P D    P D
Sbjct: 121 SSDILEALIPPKFKTPTMKPYDGSKDPKD 146

HSP 2 Score: 747.3 bits (1928), Expect = 6.0e-212
Identity = 399/551 (72.41%), Postives = 446/551 (80.94%), Query Frame = 0

Query: 187 NSNLKAQSKYKPLAPEAVITREEFDLMKHKFDEQVEALKARCEKKDCSFDNGDLGESPFT 246
           +SN +A+S + P  P+ VITREEFD ++ K + QVEALKA+CE+K+   ++GDLGESPFT
Sbjct: 2   SSNQQAESSHNPATPDGVITREEFDQLRGKLNAQVEALKAKCEQKEGPLNDGDLGESPFT 61

Query: 247 ADILEAPIPPKFKTPAMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCRVFQIALTGSAR 306
           +D+LEA        P +K YDGSKDPKDYVEVFEGLMDFQAA+DAIKCR FQIALTGSAR
Sbjct: 62  SDVLEA--------PTVKSYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR 121

Query: 307 L-KTATHLATIRQREGETLREYVTRFQEEQL-----KEAPATFAEVLQKAKKVIDGQELL 366
           L      L   +  +   +  ++T   +E L     KEAPATFAEVLQKAKKVIDGQELL
Sbjct: 122 LWFQEDQLKVAQSSDDSAMCYFLTGLADEALTVKLGKEAPATFAEVLQKAKKVIDGQELL 181

Query: 367 RTKTGQPEKQVVQRKLSQDKR------------------RTDV-----SRPYERYTPTTI 426
           RTKTG+PE+ + + +  +D++                  R  V     SRPYER+TPTTI
Sbjct: 182 RTKTGRPERGIDRGRSGKDEKADLKSKDKGSFSSGRAEFRRAVNGPTRSRPYERFTPTTI 241

Query: 427 PVSEILTNIEDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHRDHGHNTSSCWELKRQIEDL 486
           P+SEILTNIE+SGMEKLLKRPEKLRG PE+RNKDKYCRFHR+H HNTS  WELKRQIEDL
Sbjct: 242 PISEILTNIEESGMEKLLKRPEKLRGAPERRNKDKYCRFHREHDHNTSDRWELKRQIEDL 301

Query: 487 IQDGYFKKFVGKPRSNSVEKMEERKRSRTPSRREDRPAVINTIFGGPSGGQSRNKRNELA 546
           IQD YFKKFVGKPR++S EK EERK SRTP RR DRPAVINTIFGGPSGGQS +KR ELA
Sbjct: 302 IQDDYFKKFVGKPRTSSAEKKEERKLSRTPLRRIDRPAVINTIFGGPSGGQSGHKRKELA 361

Query: 547 RAARRKVCIIREQRPTFPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASAN 606
           RAARR+VCIIREQRPT PITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVD G SAN
Sbjct: 362 RAARREVCIIREQRPTCPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDEGVSAN 421

Query: 607 ILSLLTYLTLGWTRSQLKKSLTPLVGFSGESITPEGCIDLPVTFGQDKTQVTKMAEFVVI 666
           I+SLLTYL LGWTRSQLKKS TPLVGFS ES+ PEGCIDLPVT G D+TQVT+MAEFVVI
Sbjct: 422 IVSLLTYLALGWTRSQLKKSTTPLVGFSRESVIPEGCIDLPVTLGHDQTQVTQMAEFVVI 481

Query: 667 DGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTPHGVGIVRGEQTASRECYASALKGSSV 709
           DGRSAYNAIFGRPIIHSFRA+PSTLHQVLKYSTP+GVG+VRGEQ ASRECYASALKGSSV
Sbjct: 482 DGRSAYNAIFGRPIIHSFRAIPSTLHQVLKYSTPNGVGMVRGEQIASRECYASALKGSSV 541

BLAST of Moc05g27060 vs. ExPASy TrEMBL
Match: A0A6J1C7X5 (uncharacterized protein LOC111008813 OS=Momordica charantia OX=3673 GN=LOC111008813 PE=4 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 1.4e-189
Identity = 363/529 (68.62%), Postives = 399/529 (75.43%), Query Frame = 0

Query: 190 LKAQSKYKPLAPEAVITREEFDLMKHKFDEQVEALKARCEKKDCSFDNGDLGESPFTADI 249
           +KA+S   P  P  VITREEFD ++ + D QVEALKA+CE+K+   ++GDLGESPFT+D+
Sbjct: 2   VKAESSRNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDV 61

Query: 250 LEAPIPPKFKTPAMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKCRVFQIALTGSARL-- 309
           LEAPIPPKFK P +KPYDGSKDPKDYVEVFE LMDFQAA+DAIKCR F+IALTGSARL  
Sbjct: 62  LEAPIPPKFKAPTVKPYDGSKDPKDYVEVFESLMDFQAASDAIKCRAFRIALTGSARLWY 121

Query: 310 -----------------------------KTATHLATIRQREGETLREYVTRFQEEQLK- 369
                                        KTATHLATIRQ+EGETLREYVTRFQEEQLK 
Sbjct: 122 RRLPAXSISTYSQLRREFLAXFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKV 181

Query: 370 ---------------------------EAPATFAEVLQKAKKVIDGQELLRTKTGQPEKQ 429
                                      EAPATFAEVLQKAKKVIDGQELLRTKTG+PE++
Sbjct: 182 AHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERK 241

Query: 430 VVQRKLSQDKRRTDV------------------------SRPYERYTPTTIPVSEILTNI 489
           + + +  +D    D                         SRPYER+TPTTIP+SEILTNI
Sbjct: 242 IGRGRSGKDIENADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNI 301

Query: 490 EDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHRDHGHNTSSCWELKRQIEDLIQDGYFKKF 549
           E+SGMEKLLKRPEKLRG PE+R+KDKYCRFHR+HGHNTS  WELKRQIE+LIQDGYFKKF
Sbjct: 302 EESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKRQIENLIQDGYFKKF 361

Query: 550 VGKPRSNSVEKMEERKRSRTPSRREDRPAVINTIFGGPSGGQSRNKRNELARAARRKVCI 609
           VGKPR++S EK EERKRSRTP RR DRPAVINTIFGGPSGGQS  KR ELARAARR+VCI
Sbjct: 362 VGKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGRKRKELARAARREVCI 421

Query: 610 IREQRPTFPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLLTYLT 636
           IREQRPT PITFD ADLEEVHLPHNDALVIAPLIDHVVV RVLVDGG SANILSL TYL 
Sbjct: 422 IREQRPTCPITFDGADLEEVHLPHNDALVIAPLIDHVVVXRVLVDGGTSANILSLPTYLA 481

BLAST of Moc05g27060 vs. ExPASy TrEMBL
Match: A0A6J1DD03 (uncharacterized protein LOC111019899 OS=Momordica charantia OX=3673 GN=LOC111019899 PE=4 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 7.6e-167
Identity = 313/390 (80.26%), Postives = 336/390 (86.15%), Query Frame = 0

Query: 336 LKEAPATFAEVLQKAKKVIDGQELLRTKTGQPEKQVVQRKL---SQDK----------RR 395
           ++EAPATFAEVLQKAKKVIDGQELLRTK GQ             S+DK          RR
Sbjct: 18  VEEAPATFAEVLQKAKKVIDGQELLRTKIGQGRSGKDMENTDPKSKDKGSFSNGRAEYRR 77

Query: 396 TD----VSRPYERYTPTTIPVSEILTNIEDSGMEKLLKRPEKLRGDPEKRNKDKYCRFHR 455
            +     SRPYER+TPTTIP+SEILTNIE+SGMEKLLKRPEKLRG PE+R+KDKYCRFHR
Sbjct: 78  AENGPTRSRPYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHR 137

Query: 456 DHGHNTSSCWELKRQIEDLIQDGYFKKFVGKPRSNSVEKMEERKRSRTPSRREDRPAVIN 515
           +HGHNTS  WELK QIEDLIQDGYFKKFVGKPR++S EK EERKRSRTP RR DRPAVIN
Sbjct: 138 EHGHNTSDYWELKSQIEDLIQDGYFKKFVGKPRTSSAEKKEERKRSRTPPRRTDRPAVIN 197

Query: 516 TIFGGPSGGQSRNKRNELARAARRKVCIIREQRPTFPITFDSADLEEVHLPHNDALVIAP 575
           TIFGGPSGGQS +KR +LARAARR+VCIIREQRPT PITFD ADL EVHLPHNDALVIAP
Sbjct: 198 TIFGGPSGGQSGHKRKKLARAARREVCIIREQRPTCPITFDXADLXEVHLPHNDALVIAP 257

Query: 576 LIDHVVVRRVLVDGGASANILSLLTYLTLGWTRSQLKKSLTPLVGFSGESITPEGCIDLP 635
           LIDHVVVRRVLVDGGASANILSL TYL LGWTRSQLKKS TPLVGFSGES+ PEGCIDLP
Sbjct: 258 LIDHVVVRRVLVDGGASANILSLPTYLALGWTRSQLKKSPTPLVGFSGESVVPEGCIDLP 317

Query: 636 VTFGQDKTQVTKMAEFVVIDGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTPHGVGIVR 695
           VT GQD+T+VT+MAEFVV+DGRSAYNAIFGRPIIHSFRA+PSTLHQVLKYSTP+GVG VR
Sbjct: 318 VTLGQDQTRVTQMAEFVVVDGRSAYNAIFGRPIIHSFRAIPSTLHQVLKYSTPNGVGTVR 377

Query: 696 GEQTASRECYASALKGSSVCALEDQASGDG 709
           GEQTASRECYAS LKG+SVCALE   S DG
Sbjct: 378 GEQTASRECYASXLKGTSVCALETLTSRDG 407