Moc05g26800 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g26800
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	3'-5' exonuclease domain-containing protein
Location	chr5: 19336935 .. 19337456 (-)
RNA-Seq Expression	Moc05g26800
Synteny	Moc05g26800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCGACCACTGGATCTCCCGAATCCTCCGCTTCCACCGCCGCCGCCTCCACCGTCTCCTCGTCGGTCTCGACGTCGAGTGGCGCCCTAACTTCAATCGCTCTCGCCGGAACCCCGCCGCCACATTGCAGCTCTGCGTCGGCCGCCGATGCCTCATTTTCCAACTCATTCACGCCCCTGAAATCCCCCACTCTCTGCTCCACTTTCTCGGCGACGAAACCTTCACTTTCGTCGGCGTCGGAATCGATCAGGACGCTCGGAAGCTGTTCGACGACTACGATTTGACTGTGAACAACGCTGTCGATCTCCGGTCTATGGCTGTGGATCGGACGGGAAGGAGGGATTTGAGGAACGCAGGGCTGAAGGATTTGGCCAGAGAGATTCTTGGGAGGGAGATCGCGAAGCCGAAGGCCGTGACGATGAGCAGATGGGACGACGAATATCTAACTTCCGCTCAGGTAGCGTATGCTTCCATTGACGCGTTTCTGTCTTTTGAAATTGGAAGGTCTCTGTCCAGTTAG

mRNA sequence

ATGGTCGACCACTGGATCTCCCGAATCCTCCGCTTCCACCGCCGCCGCCTCCACCGTCTCCTCGTCGGTCTCGACGTCGAGTGGCGCCCTAACTTCAATCGCTCTCGCCGGAACCCCGCCGCCACATTGCAGCTCTGCGTCGGCCGCCGATGCCTCATTTTCCAACTCATTCACGCCCCTGAAATCCCCCACTCTCTGCTCCACTTTCTCGGCGACGAAACCTTCACTTTCGTCGGCGTCGGAATCGATCAGGACGCTCGGAAGCTGTTCGACGACTACGATTTGACTGTGAACAACGCTGTCGATCTCCGGTCTATGGCTGTGGATCGGACGGGAAGGAGGGATTTGAGGAACGCAGGGCTGAAGGATTTGGCCAGAGAGATTCTTGGGAGGGAGATCGCGAAGCCGAAGGCCGTGACGATGAGCAGATGGGACGACGAATATCTAACTTCCGCTCAGGTAGCGTATGCTTCCATTGACGCGTTTCTGTCTTTTGAAATTGGAAGGTCTCTGTCCAGTTAG

Coding sequence (CDS)

ATGGTCGACCACTGGATCTCCCGAATCCTCCGCTTCCACCGCCGCCGCCTCCACCGTCTCCTCGTCGGTCTCGACGTCGAGTGGCGCCCTAACTTCAATCGCTCTCGCCGGAACCCCGCCGCCACATTGCAGCTCTGCGTCGGCCGCCGATGCCTCATTTTCCAACTCATTCACGCCCCTGAAATCCCCCACTCTCTGCTCCACTTTCTCGGCGACGAAACCTTCACTTTCGTCGGCGTCGGAATCGATCAGGACGCTCGGAAGCTGTTCGACGACTACGATTTGACTGTGAACAACGCTGTCGATCTCCGGTCTATGGCTGTGGATCGGACGGGAAGGAGGGATTTGAGGAACGCAGGGCTGAAGGATTTGGCCAGAGAGATTCTTGGGAGGGAGATCGCGAAGCCGAAGGCCGTGACGATGAGCAGATGGGACGACGAATATCTAACTTCCGCTCAGGTAGCGTATGCTTCCATTGACGCGTTTCTGTCTTTTGAAATTGGAAGGTCTCTGTCCAGTTAG

Protein sequence

MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS

Homology

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BLAST of Moc05g26800 vs. NCBI nr
Match: XP_022147765.1 (Werner Syndrome-like exonuclease, partial [Momordica charantia])

HSP 1 Score: 350.1 bits (897), Expect = 1.1e-92
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP
Sbjct: 29  MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 88

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
           EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG
Sbjct: 89  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 148

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
           LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS
Sbjct: 149 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 201

[Close]

BLAST of Moc05g26800 vs. NCBI nr
Match: XP_030926963.1 (Werner Syndrome-like exonuclease [Quercus lobata])

HSP 1 Score: 251.9 bits (642), Expect = 4.0e-63
Identity = 121/173 (69.94%), Postives = 139/173 (80.35%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           MVD W+S I R HRRRLH L+VGLDVEWRP+FNR+  NP ATLQLCVGRRCLIFQLI+A 
Sbjct: 37  MVDSWLSDIYRIHRRRLHHLIVGLDVEWRPSFNRNIENPVATLQLCVGRRCLIFQLIYAT 96

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
             P+SL+ FLGDE FTFVGVGI  D  KL DDYDL V N VDLR +AVDR G RDL+NAG
Sbjct: 97  HFPNSLIEFLGDEDFTFVGVGIASDVEKLLDDYDLKVGNVVDLRGLAVDRLGNRDLKNAG 156

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
           LK LAR +LG E+ KP+ VTMSRWD+E+L   Q+ YA +DAFLSFEIGRSL++
Sbjct: 157 LKGLARAVLGLEVHKPRHVTMSRWDNEWLDYNQIQYACVDAFLSFEIGRSLNA 209

[Close]

BLAST of Moc05g26800 vs. NCBI nr
Match: KAF3954294.1 (hypothetical protein CMV_020340 [Castanea mollissima])

HSP 1 Score: 248.1 bits (632), Expect = 5.7e-62
Identity = 119/173 (68.79%), Postives = 138/173 (79.77%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           MVD WIS I R HRRRLH L+VGLDVEWRPNFNR+  NP A LQLCVGRRCLIFQLI+A 
Sbjct: 129 MVDSWISDIYRIHRRRLHHLIVGLDVEWRPNFNRNIENPVAILQLCVGRRCLIFQLIYAT 188

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
             P+SL+ FLGDE FTFVGVGI+ D  KL DD+DL V N VDLR +AVDR   RDL+NAG
Sbjct: 189 HFPNSLIEFLGDEAFTFVGVGIESDVEKLLDDHDLKVGNVVDLRGLAVDRLRNRDLKNAG 248

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
           LK LAR +LG E+ KP+ VT+SRWD+E+L   Q+ YA +DAFLSFEIGRSL++
Sbjct: 249 LKGLARAVLGLEVHKPRHVTLSRWDNEWLDYNQIQYACVDAFLSFEIGRSLNA 301

[Close]

BLAST of Moc05g26800 vs. NCBI nr
Match: XP_038906336.1 (Werner Syndrome-like exonuclease [Benincasa hispida])

HSP 1 Score: 247.7 bits (631), Expect = 7.5e-62
Identity = 120/173 (69.36%), Postives = 140/173 (80.92%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           +VD WIS ILR HRRRLHRL+VGLD+EWRPNFNR  R P ATLQLCVGRRCLIFQLIH  
Sbjct: 38  VVDMWISEILRIHRRRLHRLVVGLDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTS 97

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
           EIP SL+ FL D+TFTFVGVGIDQD ++L++DYDL V N VDLR +A +   R DL+NAG
Sbjct: 98  EIPESLIDFLEDDTFTFVGVGIDQDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAG 157

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
           LK L RE+LGREI KP+ VT+S+WD + L+  QV YA++DAFLSFEIGR L S
Sbjct: 158 LKILGREVLGREIDKPRNVTLSQWDRQCLSPGQVLYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Moc05g26800 vs. NCBI nr
Match: XP_004136771.1 (Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 245.7 bits (626), Expect = 2.8e-61
Identity = 120/173 (69.36%), Postives = 136/173 (78.61%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           MVD WIS ILR HRRRLHRL+VGLDVEWRPNF+R  RNP ATLQLCVGRRCLIFQLIHA 
Sbjct: 38  MVDMWISEILRIHRRRLHRLVVGLDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHAS 97

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
           E P SL+ FL D+TFTFVGVGID D  KL++DYDL V N VDLR +A D     + R AG
Sbjct: 98  ETPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAG 157

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
           LK L RE+LGREI KP+ V +SRWD ++L  AQ+ YA++DAFLSFEIGR L S
Sbjct: 158 LKTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Moc05g26800 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 7.4e-20
Identity = 53/152 (34.87%), Postives = 85/152 (55.92%), Query Frame = 0


Query: 22  VGLDVEWRPNFNRS-RRNPAATLQLCV-GRRCLIFQLIHAPEIPHSLLHFLGDETFTFVG 81
           VGLD+EWRP+F +       AT+Q+CV    C +  + H+  IP SL H + D T   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 82  VGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAV 141
           +GID D+ KLF DY +++ +  DL  +A  + G    +  GL  L   ++ +E+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTETLVCKELLKPNRI 249

Query: 142 TMSRWDDEYLTSAQVAYASIDAFLSFEIGRSL 172
            +  W+   L+  Q+ YA+ DA+ S+ + + L
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

[Close]

BLAST of Moc05g26800 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 2.0e-17
Identity = 50/152 (32.89%), Postives = 78/152 (51.32%), Query Frame = 0


Query: 21  LVGLDVEWRPNFNRSRRNPAATLQLCVGR-RCLIFQLIHAPEIPHSLLHFLGDETFTFVG 80
           +VG D+EW P +NR +    A +QLCV   +C +F +      P  L   L ++     G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 81  VGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAV 140
           VGI+ D  KL  D+D+ + N V+L  +A  +   +      L  L + +LG+++ K K++
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKL--KCTETWSLNSLVKHLLGKQLLKDKSI 197

Query: 141 TMSRWDDEYLTSAQVAYASIDAFLSFEIGRSL 172
             S W    LT  Q  YA+ DA+  F I R+L
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYRNL 227

[Close]

BLAST of Moc05g26800 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 85.5 bits (210), Expect = 6.4e-16
Identity = 46/154 (29.87%), Postives = 80/154 (51.95%), Query Frame = 0


Query: 21  LVGLDVEWRPNFNRSRRNPAATLQLCVGR-RCLIFQLIHAPEIPHSLLHFLGDETFTFVG 80
           +VG D+EW P +   +R+  A +QLCV   +C +F +      P  L   L +++    G
Sbjct: 72  VVGFDMEWPPIYKPGKRSRVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAG 131

Query: 81  VGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAV 140
           VGI+ D  KL  D+D+ + + V+L  +A ++   +      L  L + +LG+++ K K++
Sbjct: 132 VGIEGDQWKLLRDFDVKLESFVELTDVANEKL--KCAETWSLNGLVKHVLGKQLLKDKSI 191

Query: 141 TMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
             S W +  LT  Q  YA+ DA+    I + L +
Sbjct: 192 RCSNWSNFPLTEDQKLYAATDAYAGLIIYQKLGN 223

[Close]

BLAST of Moc05g26800 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 9.3e-15
Identity = 46/152 (30.26%), Postives = 73/152 (48.03%), Query Frame = 0


Query: 21  LVGLDVEWRPNFNRSRRNPAATLQLCVG-RRCLIFQLIHAPEIPHSLLHFLGDETFTFVG 80
           ++G D+EW P + + +    A +Q+CV  ++C +F +      P  L   L DE+   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 81  VGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAV 140
           VGI+ D  KL  DY+L +   ++L  MA  +   R         L + +   ++ K K+ 
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKL--RCKEKWTFNGLIKHLFKEQLYKRKSY 191

Query: 141 TMSRWDDEYLTSAQVAYASIDAFLSFEIGRSL 172
             S WD   LT  Q  YA+ DA+    I + L
Sbjct: 192 RCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

[Close]

BLAST of Moc05g26800 vs. ExPASy Swiss-Prot
Match: Q9NVH0 (Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens OX=9606 GN=EXD2 PE=1 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-13
Identity = 51/149 (34.23%), Postives = 73/149 (48.99%), Query Frame = 0


Query: 21  LVGLDVEWRPNFNRSRRNPAATLQLC-VGRRCLIFQL----IHAPEIPHSLLHFLGDETF 80
           ++G+D EW  N    + +P + LQ+      C++ +L         +P +LL  L D T 
Sbjct: 104 VLGIDCEW-VNL-EGKASPLSLLQMASPSGLCVLVRLPKLICGGKTLPRTLLDILADGTI 163

Query: 81  TFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAK 140
             VGVG  +DA KL  DY L V   +DLR +A+ +          LK LA  +L   + K
Sbjct: 164 LKVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMRQRNNLLCNGLSLKSLAETVLNFPLDK 223

Query: 141 PKAVTMSRWDDEYLTSAQVAYASIDAFLS 165
              +  S WD E LT  QV YA+ DA +S
Sbjct: 224 SLLLRCSNWDAETLTEDQVIYAARDAQIS 250

[Close]

BLAST of Moc05g26800 vs. ExPASy TrEMBL
Match: A0A6J1D121 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 5.2e-93
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP
Sbjct: 29  MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 88

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
           EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG
Sbjct: 89  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 148

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
           LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS
Sbjct: 149 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 201

[Close]

BLAST of Moc05g26800 vs. ExPASy TrEMBL
Match: A0A7N2M2Z8 (3'-5' exonuclease domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.8e-62
Identity = 119/172 (69.19%), Postives = 136/172 (79.07%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           MVD W+S I R HRRRLH L+VGLDVEWRP+FNR+  NP ATLQLCVGRRCLIFQLI+A 
Sbjct: 37  MVDSWLSDIYRIHRRRLHHLIVGLDVEWRPSFNRNIENPVATLQLCVGRRCLIFQLIYAT 96

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
             P+SL+ FLGDE FTFVGVGI  D  KL DDYDL V N VDLR +AVDR G RDL+NAG
Sbjct: 97  HFPNSLIEFLGDEDFTFVGVGIASDVEKLLDDYDLKVGNVVDLRGLAVDRLGNRDLKNAG 156

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLS 173
           LK LAR +LG E+ KP+ VTMSRWD+E+L   Q+ YA +DAFLSFEIG  +S
Sbjct: 157 LKGLARAVLGLEVHKPRHVTMSRWDNEWLDYNQIQYACVDAFLSFEIGSDVS 208

[Close]

BLAST of Moc05g26800 vs. ExPASy TrEMBL
Match: A0A0A0LHC5 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.4e-61
Identity = 120/173 (69.36%), Postives = 136/173 (78.61%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           MVD WIS ILR HRRRLHRL+VGLDVEWRPNF+R  RNP ATLQLCVGRRCLIFQLIHA 
Sbjct: 38  MVDMWISEILRIHRRRLHRLVVGLDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHAS 97

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
           E P SL+ FL D+TFTFVGVGID D  KL++DYDL V N VDLR +A D     + R AG
Sbjct: 98  ETPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAG 157

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
           LK L RE+LGREI KP+ V +SRWD ++L  AQ+ YA++DAFLSFEIGR L S
Sbjct: 158 LKTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Moc05g26800 vs. ExPASy TrEMBL
Match: A0A2I4GID7 (Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.8e-60
Identity = 118/173 (68.21%), Postives = 135/173 (78.03%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           +VD WIS I R HRRRLHRL+VGLD+EWRP+FNR  +NP ATLQLCVGRRCLIFQLIHAP
Sbjct: 37  LVDSWISDIYRIHRRRLHRLVVGLDLEWRPSFNRHVQNPVATLQLCVGRRCLIFQLIHAP 96

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
            IP SL+ FLG   FTFVGVGI  D  KL DDY L V   VDLR +AV+  G   LRNAG
Sbjct: 97  YIPQSLVDFLGQTDFTFVGVGIKSDVEKLLDDYGLEVACVVDLRLLAVEELGTTQLRNAG 156

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
           LK LA E+LG+ I KP+ +TMSRWD+E+LT AQV YA +DAFLSFEIGR L++
Sbjct: 157 LKQLAWEVLGKRIEKPRNITMSRWDNEWLTCAQVQYACLDAFLSFEIGRQLNA 209

[Close]

BLAST of Moc05g26800 vs. ExPASy TrEMBL
Match: A0A6A1WBZ0 (Werner Syndrome-like exonuclease OS=Morella rubra OX=262757 GN=CJ030_MR2G022295 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.7e-59
Identity = 115/173 (66.47%), Postives = 137/173 (79.19%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 60
           MVD WIS I R H RRLHRL+VGLDVEWRP+FNR  +NP ATLQLCVGRRCLIFQ++ AP
Sbjct: 38  MVDSWISDIYRIHSRRLHRLVVGLDVEWRPSFNRHVQNPVATLQLCVGRRCLIFQIVRAP 97

Query: 61  EIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAG 120
             P SL+ FLGD +FTFVGVGI+ D  KL DDY+L V N VDLR +  +  G R+LRNAG
Sbjct: 98  YFPDSLIEFLGDGSFTFVGVGIESDVEKLLDDYELEVANVVDLRDLVEEEWGIRELRNAG 157

Query: 121 LKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIGRSLSS 174
           LK LA E+LG++I KP+ VT+SRWD+E L+ AQV YA +DAFLSFEIGR L++
Sbjct: 158 LKQLAWEVLGKDIQKPRRVTLSRWDNERLSHAQVQYACLDAFLSFEIGRFLNA 210

[Close]

BLAST of Moc05g26800 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 99.4 bits (246), Expect = 3.1e-21
Identity = 53/152 (34.87%), Postives = 85/152 (55.92%), Query Frame = 0


Query: 22  VGLDVEWRPNFNRS-RRNPAATLQLCV-GRRCLIFQLIHAPEIPHSLLHFLGDETFTFVG 81
           VGLD+EWRP+F +       AT+Q+CV    C +  + H+  IP SL H + D T   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 82  VGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAV 141
           +GID D+ KLF DY +++ +  DL  +A  + G    +  GL  L   ++ +E+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTETLVCKELLKPNRI 249

Query: 142 TMSRWDDEYLTSAQVAYASIDAFLSFEIGRSL 172
            +  W+   L+  Q+ YA+ DA+ S+ + + L
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKDL 278

[Close]

BLAST of Moc05g26800 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.0e-21
Identity = 66/170 (38.82%), Postives = 85/170 (50.00%), Query Frame = 0


Query: 2   VDHWISRILRFHR-RRLHRLLVGLDVEWRPNFNRSRRNPAATLQLCVGRRCLIFQLIHAP 61
           +  WI  I  F R R  H L+VGLDV+W P        P   LQLCVG RCLI QL H  
Sbjct: 37  IRRWIHSIRFFSRLRSSHPLVVGLDVQWTPG---GSDPPPDILQLCVGNRCLIIQLSHCK 96

Query: 62  EIPHSLLHFLGDETFTFVGVGIDQDARKLFD-DYDLTVNNAVDLRSMAVDRTGRRDLRNA 121
            IP  L  FL DET TFVGV   QD  KL    + L +   +D+R     R     L N+
Sbjct: 97  RIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLEIWRLLDIRHYLPTR-----LLNS 156

Query: 122 GLKDLAREILG-REIAKPKAVTMSRWDDEYLTSAQVAYASIDAFLSFEIG 169
             + +  E LG + + K K + MS W    L+  Q+  AS D ++  ++G
Sbjct: 157 SFEKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIVQASDDVYVCCKLG 198

[Close]

BLAST of Moc05g26800 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 98.6 bits (244), Expect = 5.2e-21
Identity = 53/152 (34.87%), Postives = 85/152 (55.92%), Query Frame = 0


Query: 22  VGLDVEWRPNFNRS-RRNPAATLQLCV-GRRCLIFQLIHAPEIPHSLLHFLGDETFTFVG 81
           VGLD+EWRP+F +       AT+Q+CV    C +  + H+  IP SL H + D T   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 82  VGIDQDARKLFDDYDLTVNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAV 141
           +GID D+ KLF DY +++ +  DL  +A  + G    +  GL  L   ++ +E+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTETLVCKELLKPNRI 249

Query: 142 TMSRWDDEYLTSAQVAYASIDAFLSFEIGRSL 172
            +  W+   L+  Q+ YA+ DA+ S+ + + L
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

[Close]

BLAST of Moc05g26800 vs. TAIR 10
Match: AT3G12410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 3.4e-20
Identity = 62/187 (33.16%), Postives = 94/187 (50.27%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEW---------RPN--------FNRSRRNPAATL 60
           ++  WI  +L  +R   H L+VG+ V+W         RPN              NPA  L
Sbjct: 37  VISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYSDPRPNNYYADPPPIRYYSDNPADIL 96

Query: 61  QLCVGRRCLIFQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKLFD-DYDLTVNNAVD 120
           QLCVG RCLI QL +  ++P++L  FL D   TFVGV   QDA KL    + L +   +D
Sbjct: 97  QLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFVGVWNGQDAGKLARCCHQLEIGELLD 156

Query: 121 LRSMAVDRTGRRDLRNAGLKDLAREILGRE-IAKPKAVTMSRWDDEYLTSAQVAYASIDA 169
           +R    D  G R +R +  +++  E +G + +     ++MS W    L   Q+  AS+DA
Sbjct: 157 IRRYVTDSWG-RSMRRSSFEEIVEECMGYQGVMLDPEISMSDWTAYDLDLDQILQASLDA 216

[Close]

BLAST of Moc05g26800 vs. TAIR 10
Match: AT3G12460.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.2e-19
Identity = 62/190 (32.63%), Postives = 88/190 (46.32%), Query Frame = 0


Query: 1   MVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNR-------------------------- 60
           ++  WI  +   +R   H L+VG+ V+W P+                             
Sbjct: 36  VIGQWIHDVFFHNRLSSHPLVVGVGVQWTPSGYHPASPPVSYRSDSSPDSYRSDSPPVFY 95

Query: 61  SRRNPAATLQLCVGRRCLIFQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKL-FDDY 120
           S   PA TLQLCVG RC+I QL +   +P  L +FL D   TFVG+   QDA KL    +
Sbjct: 96  SSDPPADTLQLCVGNRCIIIQLRYCERVPQVLRNFLADRDNTFVGIWNSQDAGKLERSRH 155

Query: 121 DLTVNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGRE-IAKPKAVTMSRWDDEYLTSA 163
            L +   +DLR    D +GRR + N  L+ +  E LG   +   + V+MS W    L+  
Sbjct: 156 QLEIAELMDLREFVSDSSGRRSMYNYSLEKIVEENLGYPGVRLDREVSMSDWRVYNLSYD 215

The following BLAST results are available for this feature:

BLAST of Moc05g26800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147765.1	1.1e-92	100.00	Werner Syndrome-like exonuclease, partial [Momordica charantia]	[more]
XP_030926963.1	4.0e-63	69.94	Werner Syndrome-like exonuclease [Quercus lobata]	[more]
KAF3954294.1	5.7e-62	68.79	hypothetical protein CMV_020340 [Castanea mollissima]	[more]
XP_038906336.1	7.5e-62	69.36	Werner Syndrome-like exonuclease [Benincasa hispida]	[more]
XP_004136771.1	2.8e-61	69.36	Werner Syndrome-like exonuclease [Cucumis sativus]	[more]

BLAST of Moc05g26800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	7.4e-20	34.87	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q14191	2.0e-17	32.89	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O09053	6.4e-16	29.87	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
O93530	9.3e-15	30.26	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]
Q9NVH0	1.8e-13	34.23	Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens OX=9606 GN=EXD2 PE...	[more]

BLAST of Moc05g26800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D121	5.2e-93	100.00	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 ...	[more]
A0A7N2M2Z8	2.8e-62	69.19	3'-5' exonuclease domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]
A0A0A0LHC5	1.4e-61	69.36	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A2I4GID7	5.8e-60	68.21	Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 ...	[more]
A0A6A1WBZ0	1.7e-59	66.47	Werner Syndrome-like exonuclease OS=Morella rubra OX=262757 GN=CJ030_MR2G022295 ...	[more]

BLAST of Moc05g26800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13870.1	3.1e-21	34.87	Werner syndrome-like exonuclease	[more]
AT2G36110.1	4.0e-21	38.82	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.2	5.2e-21	34.87	Werner syndrome-like exonuclease	[more]
AT3G12410.1	3.4e-20	33.16	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12460.1	2.2e-19	32.63	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 3..172 e-value: 3.1E-4 score: 30.1
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 21..171 e-value: 1.8E-16 score: 60.4
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..172 e-value: 2.0E-57 score: 196.0
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 1..172
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 1..172
None	No IPR available	CDD	cd06141	WRN_exo	coord: 22..171 e-value: 7.13924E-64 score: 192.023
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 4..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g26800.1	Moc05g26800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding