Moc05g09320 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g09320
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionAMP dependent ligase
Locationchr5: 7237319 .. 7238983 (-)
RNA-Seq ExpressionMoc05g09320
SyntenyMoc05g09320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATCTGAAGCCAAGCCCTGCAAATTCCTCTCCTCTTACGCCAATTGGCTTCCTCGACAGAGCCGCCACCGTCTACGGCGACTGCCCTTCAGTCATCTACGGCTCCACTGCCTACACGTGGTCCCAGACCCACCGTCGATGTCTTCAACTCGCTTCTTCCCTCTCATCCAACGGCATCCACAGGGGCCACGTCGTCTCCGTCGTCGCTCCCAACACCCCCGCCATGTACGAGCTCCAGTTCGCCGTTCCGATGGCCGGCGCCGTCCTCAACAACATCAATCTCCGCCTCGACGCTCGTACCATCTCTATCCTCCTCCGCCATAGCGAATCCAAGCTCGTCTTCGTCGATCAAGCCTCTTGCTCCCTTATCTCCGACGCCTTGGCCTTGTTTCCGCCGGAAGCCAGACGGCCGGCGCTCGTCCTGATCGCCGATGACGCTGCGGCGGAGGAGTCGTCTTCTTCACCGGTTTCCGGAGATTTCGTCGACACGTATGAGGGAATGGTGGAGAAAGGGAATCCAGAGTTCCGGTGGATTCGGCCGGCGAGTGATTGGGATCCGATTGTTTTGAATTACACTTCGGGAACGACGTCGTCGCCGAAAGGAGTGGTCCATTGCCACAGAGGAGTTTTCATGGTAGCTCTGGATTCGCTTCTAGAATGGGGCGTCCCTAAGCAATCAGTTTATTTATGGACTTTGCCGATCTTCCACGCCAATGGATGGTGCTTCCCGTGGGGAATCGCCGCCATAGGCGGCACAAATGTCTGTCTCAGAAAATTCGACGCGCCATTGATTTTTCGCCTCATTCATCGGCACAACGTGACGCACATGTGCGGAGCTCCAGTGGTGCTCAATATGCTCACGAACTCCCCGAAGAATAAGCCGCTCGATCGGCCAGTGAAGATCCTCACCGCCGGCGCGCCGCCGCCTGCAGCGGTTCTGTTCCGCACCGAATCGTTGGGTTTCGATGTGAGTCATGGGTACGGACTAACAGAGACCGCCGGGCTGGTGGTGGTGTGCTCGTGGAAGCCGCAGTGGAAGCGGCTACCGGCGGCGGAGAGAGCGCGGTTGAAGGCGCGACAGGGGGTGAGAACGATGGCAATAACAGAGGCGGACGTTGTGGATCCGGAGACTGGCAAGAGCGTGAAACGCGACGGATCGTCAATAGGAGAGATCGTTCTAAAAGGTGGATCGATCATGCTCGGTTACCTCAAAGATCCGGAAGCGACATCCAAATCGATGACCGAGGAGGGGTGGTTCTTCACAGGAGACGTCGGCGTGATGCACGGCGACGGGTATTTGGAGATCAAGGACCGATCGAAGGACGTGATCATAAGCGGAGGAGAGAACCTGAGCAGCGTGGAGGTAGAATCGGTACTGTACACAAATCCGGCGGTGAACGAGGCGGCGGTGGTGGCGCGACCGGATGAGTTCTGGGGGGAGACGCCGTGCGCGTTCGTGAGCTTGAGAGAAGGATTGAGTCGGAGGCCGACGGAGGAAGAGATAATTGAGTACTGCAGAGGGAAGCTGCCGCGGTTTATGGTGCCGAAGACGGTGGTTTTCAAGGCAGAACTGCCGAAGACGTCGACGGGGAAGATCAAGAAGTTCGTGCTTAGGGAGATTGCCAAGTCGATGGGCCCTGCACGGGCGAGTCGCTTGTAG

mRNA sequence

ATGGAAGATCTGAAGCCAAGCCCTGCAAATTCCTCTCCTCTTACGCCAATTGGCTTCCTCGACAGAGCCGCCACCGTCTACGGCGACTGCCCTTCAGTCATCTACGGCTCCACTGCCTACACGTGGTCCCAGACCCACCGTCGATGTCTTCAACTCGCTTCTTCCCTCTCATCCAACGGCATCCACAGGGGCCACGTCGTCTCCGTCGTCGCTCCCAACACCCCCGCCATGTACGAGCTCCAGTTCGCCGTTCCGATGGCCGGCGCCGTCCTCAACAACATCAATCTCCGCCTCGACGCTCGTACCATCTCTATCCTCCTCCGCCATAGCGAATCCAAGCTCGTCTTCGTCGATCAAGCCTCTTGCTCCCTTATCTCCGACGCCTTGGCCTTGTTTCCGCCGGAAGCCAGACGGCCGGCGCTCGTCCTGATCGCCGATGACGCTGCGGCGGAGGAGTCGTCTTCTTCACCGGTTTCCGGAGATTTCGTCGACACGTATGAGGGAATGGTGGAGAAAGGGAATCCAGAGTTCCGGTGGATTCGGCCGGCGAGTGATTGGGATCCGATTGTTTTGAATTACACTTCGGGAACGACGTCGTCGCCGAAAGGAGTGGTCCATTGCCACAGAGGAGTTTTCATGGTAGCTCTGGATTCGCTTCTAGAATGGGGCGTCCCTAAGCAATCAGTTTATTTATGGACTTTGCCGATCTTCCACGCCAATGGATGGTGCTTCCCGTGGGGAATCGCCGCCATAGGCGGCACAAATGTCTGTCTCAGAAAATTCGACGCGCCATTGATTTTTCGCCTCATTCATCGGCACAACGTGACGCACATGTGCGGAGCTCCAGTGGTGCTCAATATGCTCACGAACTCCCCGAAGAATAAGCCGCTCGATCGGCCAGTGAAGATCCTCACCGCCGGCGCGCCGCCGCCTGCAGCGGTTCTGTTCCGCACCGAATCGTTGGGTTTCGATGTGAGTCATGGGTACGGACTAACAGAGACCGCCGGGCTGGTGGTGGTGTGCTCGTGGAAGCCGCAGTGGAAGCGGCTACCGGCGGCGGAGAGAGCGCGGTTGAAGGCGCGACAGGGGGTGAGAACGATGGCAATAACAGAGGCGGACGTTGTGGATCCGGAGACTGGCAAGAGCGTGAAACGCGACGGATCGTCAATAGGAGAGATCGTTCTAAAAGGTGGATCGATCATGCTCGGTTACCTCAAAGATCCGGAAGCGACATCCAAATCGATGACCGAGGAGGGGTGGTTCTTCACAGGAGACGTCGGCGTGATGCACGGCGACGGGTATTTGGAGATCAAGGACCGATCGAAGGACGTGATCATAAGCGGAGGAGAGAACCTGAGCAGCGTGGAGGTAGAATCGGTACTGTACACAAATCCGGCGGTGAACGAGGCGGCGGTGGTGGCGCGACCGGATGAGTTCTGGGGGGAGACGCCGTGCGCGTTCGTGAGCTTGAGAGAAGGATTGAGTCGGAGGCCGACGGAGGAAGAGATAATTGAGTACTGCAGAGGGAAGCTGCCGCGGTTTATGGTGCCGAAGACGGTGGTTTTCAAGGCAGAACTGCCGAAGACGTCGACGGGGAAGATCAAGAAGTTCGTGCTTAGGGAGATTGCCAAGTCGATGGGCCCTGCACGGGCGAGTCGCTTGTAG

Coding sequence (CDS)

ATGGAAGATCTGAAGCCAAGCCCTGCAAATTCCTCTCCTCTTACGCCAATTGGCTTCCTCGACAGAGCCGCCACCGTCTACGGCGACTGCCCTTCAGTCATCTACGGCTCCACTGCCTACACGTGGTCCCAGACCCACCGTCGATGTCTTCAACTCGCTTCTTCCCTCTCATCCAACGGCATCCACAGGGGCCACGTCGTCTCCGTCGTCGCTCCCAACACCCCCGCCATGTACGAGCTCCAGTTCGCCGTTCCGATGGCCGGCGCCGTCCTCAACAACATCAATCTCCGCCTCGACGCTCGTACCATCTCTATCCTCCTCCGCCATAGCGAATCCAAGCTCGTCTTCGTCGATCAAGCCTCTTGCTCCCTTATCTCCGACGCCTTGGCCTTGTTTCCGCCGGAAGCCAGACGGCCGGCGCTCGTCCTGATCGCCGATGACGCTGCGGCGGAGGAGTCGTCTTCTTCACCGGTTTCCGGAGATTTCGTCGACACGTATGAGGGAATGGTGGAGAAAGGGAATCCAGAGTTCCGGTGGATTCGGCCGGCGAGTGATTGGGATCCGATTGTTTTGAATTACACTTCGGGAACGACGTCGTCGCCGAAAGGAGTGGTCCATTGCCACAGAGGAGTTTTCATGGTAGCTCTGGATTCGCTTCTAGAATGGGGCGTCCCTAAGCAATCAGTTTATTTATGGACTTTGCCGATCTTCCACGCCAATGGATGGTGCTTCCCGTGGGGAATCGCCGCCATAGGCGGCACAAATGTCTGTCTCAGAAAATTCGACGCGCCATTGATTTTTCGCCTCATTCATCGGCACAACGTGACGCACATGTGCGGAGCTCCAGTGGTGCTCAATATGCTCACGAACTCCCCGAAGAATAAGCCGCTCGATCGGCCAGTGAAGATCCTCACCGCCGGCGCGCCGCCGCCTGCAGCGGTTCTGTTCCGCACCGAATCGTTGGGTTTCGATGTGAGTCATGGGTACGGACTAACAGAGACCGCCGGGCTGGTGGTGGTGTGCTCGTGGAAGCCGCAGTGGAAGCGGCTACCGGCGGCGGAGAGAGCGCGGTTGAAGGCGCGACAGGGGGTGAGAACGATGGCAATAACAGAGGCGGACGTTGTGGATCCGGAGACTGGCAAGAGCGTGAAACGCGACGGATCGTCAATAGGAGAGATCGTTCTAAAAGGTGGATCGATCATGCTCGGTTACCTCAAAGATCCGGAAGCGACATCCAAATCGATGACCGAGGAGGGGTGGTTCTTCACAGGAGACGTCGGCGTGATGCACGGCGACGGGTATTTGGAGATCAAGGACCGATCGAAGGACGTGATCATAAGCGGAGGAGAGAACCTGAGCAGCGTGGAGGTAGAATCGGTACTGTACACAAATCCGGCGGTGAACGAGGCGGCGGTGGTGGCGCGACCGGATGAGTTCTGGGGGGAGACGCCGTGCGCGTTCGTGAGCTTGAGAGAAGGATTGAGTCGGAGGCCGACGGAGGAAGAGATAATTGAGTACTGCAGAGGGAAGCTGCCGCGGTTTATGGTGCCGAAGACGGTGGTTTTCAAGGCAGAACTGCCGAAGACGTCGACGGGGAAGATCAAGAAGTTCGTGCTTAGGGAGATTGCCAAGTCGATGGGCCCTGCACGGGCGAGTCGCTTGTAG

Protein sequence

MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNGIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVLREIAKSMGPARASRL
Homology
BLAST of Moc05g09320 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 965.7 bits (2495), Expect = 1.7e-277
Identity = 469/557 (84.20%), Postives = 512/557 (91.92%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNT AMYEL FAVPMAGAVLN INLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPV---SGDFVDTYEGMVEKGNPEF 180
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSS+       DFVD+YE MV+KG+P F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
           +WIRPAS+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
           HANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP N+PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
           DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAAERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
           LKARQGVRT+A+  ADV+DPETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K+MT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
           EFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLREIAKSMGPARASRL 555
           VLRE+AKSMGPA  SRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of Moc05g09320 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 962.2 bits (2486), Expect = 1.9e-276
Identity = 469/561 (83.60%), Postives = 511/561 (91.09%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNTPAMYEL FAVPMAGAVLN INLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS-------GDFVDTYEGMVEKG 180
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSSS  S        DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 240
           +P F+WIRP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 300
           LP+FHANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP 
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 360
           N+PLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAA
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 420
           ERARLKARQGVRT+A+  ADV+ PETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 SMTEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
           +MT EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 540
           ARPDEFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 IKKFVLREIAKSMGPARASRL 555
           I+KFVLRE+AKSMGPA  SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of Moc05g09320 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 962.2 bits (2486), Expect = 1.9e-276
Identity = 469/561 (83.60%), Postives = 512/561 (91.27%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           MEDLKP+PANSSPLTPIGFL+RAA VYGDC S+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNT AMYEL FAVPMAGAVLN INLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS-------GDFVDTYEGMVEKG 180
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSSS  S        DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 181 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 240
           +P F+WIRPAS+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 241 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 300
           LP+FHANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP 
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 360
           N+PLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAA
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 420
           +RARLKARQGVRT+A+  ADV+DPETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 SMTEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
           +MT EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 540
           ARPDEFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540

Query: 541 IKKFVLREIAKSMGPARASRL 555
           I+KFVLRE+AKSMGPA  SRL
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561

BLAST of Moc05g09320 vs. NCBI nr
Match: XP_038877068.1 (probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida])

HSP 1 Score: 951.0 bits (2457), Expect = 4.4e-273
Identity = 461/558 (82.62%), Postives = 510/558 (91.40%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           MEDLKPS ANSSPLTPIGFL+RA+ +YGD PS+IY ST YTWS+T+RRCL++ASSLSS G
Sbjct: 28  MEDLKPSLANSSPLTPIGFLERASVIYGDSPSIIYNSTTYTWSETYRRCLRVASSLSSIG 87

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           I +G+VVSV+APNTP MYELQFAVPMAGAVLN INLRLDARTISILLRH ESKL+F+DQA
Sbjct: 88  IQKGYVVSVIAPNTPPMYELQFAVPMAGAVLNCINLRLDARTISILLRHCESKLIFIDQA 147

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEE----SSSSPVSGDFVDTYEGMVEKGNPE 180
           SC LISDALALFPPE +RP LVLIADD A E+    SSSS + G+FVD+YE MV+KG+ E
Sbjct: 148 SCGLISDALALFPPETKRPLLVLIADDTAVEDSLSSSSSSVIGGEFVDSYEEMVQKGDDE 207

Query: 181 FRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPI 240
           FRWIRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPKQSVYLWTLP+
Sbjct: 208 FRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFAVTLDSLIEWGVPKQSVYLWTLPM 267

Query: 241 FHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKP 300
           FHANGW FP+GIAA+GGTNVC+RKFDA LIF LIHRH+VTH+CGAPVVLNMLTNSP N+P
Sbjct: 268 FHANGWSFPFGIAAVGGTNVCIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPDNQP 327

Query: 301 LDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERA 360
           L+RPVKILTAGAPPPAAVLFRTESLGFDV HGYGLTET GLV+ CSWK +W RLPA ERA
Sbjct: 328 LERPVKILTAGAPPPAAVLFRTESLGFDVGHGYGLTETGGLVIYCSWKSKWNRLPATERA 387

Query: 361 RLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMT 420
           RLKARQGVRT+A++E DV+D ETGKSVKRDG+SIGEIVL+GGS+MLGY KDPEAT K+MT
Sbjct: 388 RLKARQGVRTLAMSEVDVLDSETGKSVKRDGASIGEIVLRGGSMMLGYFKDPEATKKAMT 447

Query: 421 EEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 480
           +EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP
Sbjct: 448 DEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 507

Query: 481 DEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKK 540
           DEFWGETPCAFVSLREGL+R PTEEEI+EYCR KLP+FMVPKTVVF  +LPKTSTGKI+K
Sbjct: 508 DEFWGETPCAFVSLREGLTRLPTEEEIMEYCREKLPKFMVPKTVVFMEKLPKTSTGKIQK 567

Query: 541 FVLREIAKSMGPARASRL 555
           F+LRE AKSMGPAR SRL
Sbjct: 568 FLLREKAKSMGPARTSRL 585

BLAST of Moc05g09320 vs. NCBI nr
Match: XP_008463175.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046215.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18981.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 938.7 bits (2425), Expect = 2.3e-269
Identity = 454/555 (81.80%), Postives = 508/555 (91.53%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           M+ LKP+PANSSPLTPI FL+RA+ VY D PS+IY ST++TWSQT+ RCL+LASSLSS G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           IH+GHVVSV++PNTP MYELQF+VPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 180
           S +LISDAL LFPPE++RP LVLIADDAA E+S+SS  + GDFVD YE M++KG+PEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
           IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 300
           NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTH+CGAPVVLNMLTNSP N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 360
           PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 361 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEG 420
           ARQGVRT+A+ EADV+D ETGKSVKRDG SIGEIVL+GGS+MLGY KDPEAT K+MTEEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 421 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
           WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 540
           WGETPCAFVSLREGLSR PTEEEIIEYCRGKL  FMVPKTVVF  +LPKTSTGKI+KF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 541 REIAKSMGPARASRL 555
           RE+AKSMGP+R SRL
Sbjct: 541 REMAKSMGPSRKSRL 555

BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 1.1e-245
Identity = 395/551 (71.69%), Postives = 483/551 (87.66%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           MEDLKP PA+SSPLTP+GFL+RAATVYGDC SV+Y + +YTWSQTHRRCL LASS++S G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           I  GHVVSV+APN P MYEL FAVPMAGA+LN +NLRLDARTISILL HSESKL+FVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRWI 180
           S  LI +A+ALFP +A  P LV +AD+  +E  +SS +  +F  +Y+ ++++G+P+F+W+
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADE--SESGNSSELGKEFFCSYKDLIDRGDPDFKWV 180

Query: 181 RPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHAN 240
            P S+WDP++LNYTSGTTSSPKGVVHCHRG+F++ +DSL++WGVPKQ VYLWTLP+FHAN
Sbjct: 181 MPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHAN 240

Query: 241 GWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDRP 300
           GW +PWG+AA+GGTN+CLRKFD+ +I+ +I RH VTHMCGAPVVLNML+N+P ++PL   
Sbjct: 241 GWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKTT 300

Query: 301 VKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLKA 360
           V+I+TAGAPPP+AVLFRTESLGF VSHGYGLTETAGLVV C+WK +W  LPA ERARLK+
Sbjct: 301 VQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARLKS 360

Query: 361 RQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEGW 420
           RQGV T+  T+ DVVDP TG +VKRDGS++GE+VL+GGS+MLGYLKDPE T+KSMT +GW
Sbjct: 361 RQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTADGW 420

Query: 421 FFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEFW 480
           F+TGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVES+LY++P + EAAVVARPDEFW
Sbjct: 421 FYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDEFW 480

Query: 481 GETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVLR 540
           GETPCAFVSL++GL+++PTE+EI+EYCR KLPR+MVPKTVVFK ELPKTSTGK++KF+LR
Sbjct: 481 GETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFILR 540

Query: 541 EIAKSMGPARA 552
           ++A+ MG A A
Sbjct: 541 DMARGMGSATA 549

BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 3.9e-232
Identity = 389/557 (69.84%), Postives = 468/557 (84.02%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 60
           ME +KP  ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSG--DFVDTYEGMVEKGNPEF 180
            S  L  +A+++   +   P LV IAD    EE   + V+    F  TY+ ++ +G+ +F
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK  VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
           HANGW +PWGIAA+GGTNVCLRKFDAPLI+RLI  H VTHMCGAPVVLNML+ + + +PL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
           +RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTETAGL V C+WKPQW RLPA++RAR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
           LKARQGVRT+  TE DVVDPE+G+SV+R+G ++GEIV++G SIMLGYLKDP  T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-K 420

Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
            FWGETPCAFVSL+ GL++RPTE E+IEYCR K+P++MVPKTV F  ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 541 VLREIAKSMGPARASRL 555
           VLREIAK MG  R SR+
Sbjct: 541 VLREIAKKMGTTRLSRM 552

BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 1.5e-228
Identity = 378/550 (68.73%), Postives = 464/550 (84.36%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 60
           ME++KP  ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS--GDFVDTYEGMVEKGNPEF 180
            S  L  +A+++   +   P LV+IAD    EE   + V+    F  TY+ ++E+G+P F
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK  VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
           H+NGW  PWGIAA+GGTNVCLRKFDAPLI+RLI  H VTHMCGAPVVLNML+ + +++PL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
           + PV ILTAG+PPPA VL R ES+GF +SHGYGLTETAG++V C+WKP+W  LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
           LKARQGVRT+  TE DVVDPE+G SV+R+G ++GEIV++G S+MLGYLKDP  T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-K 420

Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
           EFWGETPCAFVSL+ G S +PTEEE++EYCR K+P++MVPKTV F  ELPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 541 VLREIAKSMG 548
           VLR+IAK MG
Sbjct: 541 VLRDIAKKMG 545

BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 3.0e-224
Identity = 380/554 (68.59%), Postives = 453/554 (81.77%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSS-N 60
           ME+LKP  ANS PLTP+GFL+RAATVYGDC SV+Y   +YTWSQTH RCL++AS + S  
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
           G+ +G VVSVVAPN PAMYEL FAVPMAG VLNNIN RL+A TIS++LRHSESKLVFVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPE-FR 180
            S  L+ DA++LFP     P LVLIAD+   E  +       FV  YE +VEKG+ + F+
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGENLTVED-REHFVCCYEDLVEKGDDKTFK 180

Query: 181 WIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFH 240
           W+RP S+WDPIVLNYTSGTTSSPKGVVH HR VF+V LDSL++WGVPKQ VYLWTLP+FH
Sbjct: 181 WVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMFH 240

Query: 241 ANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLD 300
           ANGW + WGIAA+GGTN+CLR+FD  +IF LI RH VTHMC AP+VLNML+NSP  +PL 
Sbjct: 241 ANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPLP 300

Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARL 360
            PV ++T GAPPPAAVL RTESLGF +SHGYG+TE  G+VV C+WK +W RLPA E+ARL
Sbjct: 301 NPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQARL 360

Query: 361 KARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEE 420
           K+RQGVRT A+ E DVVDP +G SVKRDG ++GEIVLKG SIMLGYLK+  AT+K +  +
Sbjct: 361 KSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIRAD 420

Query: 421 GWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
           GWF+TGD+ VMH DGYLEIKDRSKDVIISGGEN+SSVEVES LY++PAV+EAAVVA PDE
Sbjct: 421 GWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACPDE 480

Query: 481 FWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFV 540
           +WGETP AFV+L++G+  RPTE+EI+EYCR KL  FMVPK VVF+ +LPKTSTGKI+KF 
Sbjct: 481 YWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQKFT 540

Query: 541 LREIAKSMGPARAS 553
           L+EI K+MG + +S
Sbjct: 541 LKEIVKTMGYSSSS 553

BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 1.9e-218
Identity = 369/548 (67.34%), Postives = 446/548 (81.39%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYG-STAYTWSQTHRRCLQLASSLSSN 60
           MEDLKPS ANS PLT +GFL+RAATVYGDC S++YG ST YTW +T+ RCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++ NTP MYELQF+VPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRW 180
               L  +A+ +       P LVLIA++   E  +       F   Y  ++ +GNP+F+W
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFKW 180

Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
           IRP S+WDPIV+NYTSGTTSSPKGVVHCHRG+F++ LDSL +W VPK  VYLWTLPIFHA
Sbjct: 181 IRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFHA 240

Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK-NKPLD 300
           NGW +PWGIAA+GGTNVC+RK  AP I+ LI  H VTHM GAP+VL +L+ S + ++PL 
Sbjct: 241 NGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPLK 300

Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARL 360
            PV  LTAG+ PPA VL R ESLGF VSHGYGLTETAG++V C+WKP W RLPA+++A+L
Sbjct: 301 SPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQL 360

Query: 361 KARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEE 420
           K+RQGVRT+  +E DVVDPE+G+SV+RDG ++GEIVL+G SIMLGYLK+P  T  S  + 
Sbjct: 361 KSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-KN 420

Query: 421 GWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
           GWFFTGD+GV+HGDGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPDE
Sbjct: 421 GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFV 540
           FWGETPCAFVSL+ GL+R+PT++EIIEYC+ K+PR+M PKTV F  ELPKTSTGKI K +
Sbjct: 481 FWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKSL 540

Query: 541 LREIAKSM 547
           L+EIAK+M
Sbjct: 541 LKEIAKNM 544

BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 8.3e-278
Identity = 469/557 (84.20%), Postives = 512/557 (91.92%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNT AMYEL FAVPMAGAVLN INLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPV---SGDFVDTYEGMVEKGNPEF 180
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSS+       DFVD+YE MV+KG+P F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
           +WIRPAS+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
           HANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP N+PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
           DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAAERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
           LKARQGVRT+A+  ADV+DPETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K+MT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
           EFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLREIAKSMGPARASRL 555
           VLRE+AKSMGPA  SRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 9.2e-277
Identity = 469/561 (83.60%), Postives = 511/561 (91.09%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           I RG VVSVV+PNTPAMYEL FAVPMAGAVLN INLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS-------GDFVDTYEGMVEKG 180
           SC LISDALALFPPE +RP L+LIAD+AAAE+SSSS  S        DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 240
           +P F+WIRP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 300
           LP+FHANGW FPWGIAA+GGTNVC+RKFDA  IF LIHR++VTH+CGAPVVLNMLTNSP 
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 360
           N+PLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK  W RLPAA
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 420
           ERARLKARQGVRT+A+  ADV+ PETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 SMTEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
           +MT EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 540
           ARPDEFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 IKKFVLREIAKSMGPARASRL 555
           I+KFVLRE+AKSMGPA  SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match: A0A5A7TXV0 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00770 PE=4 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 454/555 (81.80%), Postives = 508/555 (91.53%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           M+ LKP+PANSSPLTPI FL+RA+ VY D PS+IY ST++TWSQT+ RCL+LASSLSS G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           IH+GHVVSV++PNTP MYELQF+VPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 180
           S +LISDAL LFPPE++RP LVLIADDAA E+S+SS  + GDFVD YE M++KG+PEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
           IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 300
           NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTH+CGAPVVLNMLTNSP N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 360
           PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 361 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEG 420
           ARQGVRT+A+ EADV+D ETGKSVKRDG SIGEIVL+GGS+MLGY KDPEAT K+MTEEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 421 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
           WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 540
           WGETPCAFVSLREGLSR PTEEEIIEYCRGKL  FMVPKTVVF  +LPKTSTGKI+KF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 541 REIAKSMGPARASRL 555
           RE+AKSMGP+R SRL
Sbjct: 541 REMAKSMGPSRKSRL 555

BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match: A0A1S3CIM6 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501383 PE=4 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 454/555 (81.80%), Postives = 508/555 (91.53%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           M+ LKP+PANSSPLTPI FL+RA+ VY D PS+IY ST++TWSQT+ RCL+LASSLSS G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           IH+GHVVSV++PNTP MYELQF+VPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 180
           S +LISDAL LFPPE++RP LVLIADDAA E+S+SS  + GDFVD YE M++KG+PEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
           IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 300
           NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTH+CGAPVVLNMLTNSP N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 360
           PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 361 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEG 420
           ARQGVRT+A+ EADV+D ETGKSVKRDG SIGEIVL+GGS+MLGY KDPEAT K+MTEEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 421 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
           WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 540
           WGETPCAFVSLREGLSR PTEEEIIEYCRGKL  FMVPKTVVF  +LPKTSTGKI+KF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 541 REIAKSMGPARASRL 555
           RE+AKSMGP+R SRL
Sbjct: 541 REMAKSMGPSRKSRL 555

BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match: A0A0A0KT33 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G317890 PE=4 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 8.7e-267
Identity = 451/555 (81.26%), Postives = 503/555 (90.63%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
           ME LKP+PANSSPLTPIGFL+RA+ +Y D PS+IY ST +TWSQT+RRCL+LASSLSS G
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
           IH+GHVVSV+APNTP MYELQFAVPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 180
           S  LISDALALFPPEA+RP LVLI+DDAA E+S+SS  + GD VD YE MV+KG+PEFRW
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFRW 201

Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
           IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 202 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 261

Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 300
           NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTHMCGAPVVLNMLTNSP N+PL+R
Sbjct: 262 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLER 321

Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 360
           PVKILTAGAPPPAAVL RTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 322 PVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARLK 381

Query: 361 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEG 420
           ARQGVRT+A+ EADV+D  TGK V+RDG SIGEIV++GGS+MLGY KDPEAT K+MTEEG
Sbjct: 382 ARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEEG 441

Query: 421 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
           WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 442 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501

Query: 481 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 540
           WGETPCAFVSLREGL+R PTEEEIIEYC+GKL  FMVPKTVVF  +LPKTSTGKI+KF+L
Sbjct: 502 WGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 561

Query: 541 REIAKSMGPARASRL 555
           RE+AKSMG +  SRL
Sbjct: 562 REMAKSMGCSPKSRL 576

BLAST of Moc05g09320 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 805.4 bits (2079), Expect = 2.8e-233
Identity = 389/557 (69.84%), Postives = 468/557 (84.02%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 60
           ME +KP  ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSG--DFVDTYEGMVEKGNPEF 180
            S  L  +A+++   +   P LV IAD    EE   + V+    F  TY+ ++ +G+ +F
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK  VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
           HANGW +PWGIAA+GGTNVCLRKFDAPLI+RLI  H VTHMCGAPVVLNML+ + + +PL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
           +RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTETAGL V C+WKPQW RLPA++RAR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
           LKARQGVRT+  TE DVVDPE+G+SV+R+G ++GEIV++G SIMLGYLKDP  T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-K 420

Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
            FWGETPCAFVSL+ GL++RPTE E+IEYCR K+P++MVPKTV F  ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 541 VLREIAKSMGPARASRL 555
           VLREIAK MG  R SR+
Sbjct: 541 VLREIAKKMGTTRLSRM 552

BLAST of Moc05g09320 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 793.5 bits (2048), Expect = 1.1e-229
Identity = 378/550 (68.73%), Postives = 464/550 (84.36%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 60
           ME++KP  ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS--GDFVDTYEGMVEKGNPEF 180
            S  L  +A+++   +   P LV+IAD    EE   + V+    F  TY+ ++E+G+P F
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK  VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
           H+NGW  PWGIAA+GGTNVCLRKFDAPLI+RLI  H VTHMCGAPVVLNML+ + +++PL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
           + PV ILTAG+PPPA VL R ES+GF +SHGYGLTETAG++V C+WKP+W  LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
           LKARQGVRT+  TE DVVDPE+G SV+R+G ++GEIV++G S+MLGYLKDP  T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-K 420

Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
            GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
           EFWGETPCAFVSL+ G S +PTEEE++EYCR K+P++MVPKTV F  ELPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 541 VLREIAKSMG 548
           VLR+IAK MG
Sbjct: 541 VLRDIAKKMG 545

BLAST of Moc05g09320 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 760.0 bits (1961), Expect = 1.3e-219
Identity = 369/548 (67.34%), Postives = 446/548 (81.39%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYG-STAYTWSQTHRRCLQLASSLSSN 60
           MEDLKPS ANS PLT +GFL+RAATVYGDC S++YG ST YTW +T+ RCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
           GI R  VVSV++ NTP MYELQF+VPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRW 180
               L  +A+ +       P LVLIA++   E  +       F   Y  ++ +GNP+F+W
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFKW 180

Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
           IRP S+WDPIV+NYTSGTTSSPKGVVHCHRG+F++ LDSL +W VPK  VYLWTLPIFHA
Sbjct: 181 IRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFHA 240

Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK-NKPLD 300
           NGW +PWGIAA+GGTNVC+RK  AP I+ LI  H VTHM GAP+VL +L+ S + ++PL 
Sbjct: 241 NGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPLK 300

Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARL 360
            PV  LTAG+ PPA VL R ESLGF VSHGYGLTETAG++V C+WKP W RLPA+++A+L
Sbjct: 301 SPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQL 360

Query: 361 KARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEE 420
           K+RQGVRT+  +E DVVDPE+G+SV+RDG ++GEIVL+G SIMLGYLK+P  T  S  + 
Sbjct: 361 KSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-KN 420

Query: 421 GWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
           GWFFTGD+GV+HGDGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPDE
Sbjct: 421 GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFV 540
           FWGETPCAFVSL+ GL+R+PT++EIIEYC+ K+PR+M PKTV F  ELPKTSTGKI K +
Sbjct: 481 FWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKSL 540

Query: 541 LREIAKSM 547
           L+EIAK+M
Sbjct: 541 LKEIAKNM 544

BLAST of Moc05g09320 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 731.5 bits (1887), Expect = 5.1e-211
Identity = 350/552 (63.41%), Postives = 445/552 (80.62%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIY-GSTAYTWSQTHRRCLQLASSLSSN 60
           ME L P P+NS+PLT +GFLDRAA+VYGDCPS+++  +T +TWS+TH RCL++AS+L+S+
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  --GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFV 120
             GI+RG VVSVV PN P++YELQFAVPM+GA+LNNIN RLDA  +S+LLRHSESKLVFV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQASCSLISDALALFPPEARRPALVLIADDAAAEESSSSP-VSGDFVDTYEGMVEKGNPE 180
           D  S S++ +A++ F  +  +P LVL+ DD   E+ S SP  + DF+DTY+G++E+G+  
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAASDFLDTYQGVMERGDSR 180

Query: 181 FRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPI 240
           F+WIRP ++W P++LNYTSGTTSSPKGVV  HR +FM+ + SLL+W  P + VYLWTLP+
Sbjct: 181 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 240

Query: 241 FHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKP 300
           FHANGW + WG AA+G TNVC R+ DAP I+ LI +H+VTHMC AP+VLNMLTN P  KP
Sbjct: 241 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 300

Query: 301 LDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERA 360
           L  PV+++TAGAPPPAA++ R E+LGF+V HGYGLTET G VV C+WK +W  L   ERA
Sbjct: 301 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 360

Query: 361 RLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMT 420
           RLK+RQGVRT+   E DV DP TGKSV+ DG S+GEIVLKGGS+MLGY KDPE T+  M 
Sbjct: 361 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 420

Query: 421 EEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 480
           E+GWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLYTNP V EAAVVA+P
Sbjct: 421 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 480

Query: 481 DEFWGETPCAFVSLREGLSRR--PTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKI 540
           D+ WGETPCAFVSL+   +     TE EI E+C+ +LP++MVP+ V+F+ ELPKTSTGKI
Sbjct: 481 DKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGKI 540

Query: 541 KKFVLREIAKSM 547
           +KF+LR++AKS+
Sbjct: 541 QKFLLRQMAKSL 549

BLAST of Moc05g09320 vs. TAIR 10
Match: AT1G21530.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 729.2 bits (1881), Expect = 2.5e-210
Identity = 348/553 (62.93%), Postives = 444/553 (80.29%), Query Frame = 0

Query: 1   MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIY-GSTAYTWSQTHRRCLQLASSLSSN 60
           ME L P P+NS+PLT +GFLDRAA+VYGDCPS+++  +T +TWS+TH RCL++AS+L+S+
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  --GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFV 120
             GI +G VVSVV PN P++YELQFAVPM+GA+LNNIN RLDA  +S+LLRHSES+LVFV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEF 180
           D  S SL+ +A++LF  +  +P LVL+ DD   + SS+S    DF+DTYE ++E+GN  F
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLLDDDQENDSSSAS----DFLDTYEEIMERGNSRF 180

Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP ++W P+VLNYTSGTTSSPKGVV  HR +FM+ + SLL+W VP + VYLWTLP+F
Sbjct: 181 KWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTLPMF 240

Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
           HANGW + WG AA+G TN+C R+ DAP I+ LI +HNVTHMC AP+VLNML N P + PL
Sbjct: 241 HANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLSTPL 300

Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
             PV+++T+GAPPPA ++ R ESLGF+VSH YGLTET+G VV C+WKP+W  L   ERAR
Sbjct: 301 KNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLERAR 360

Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
           LK+RQGVRT+  TE DV D +TGKSVK DG S+GEIV +G S+MLGY KDP+ T+  M E
Sbjct: 361 LKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAACMRE 420

Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
           +GWF++GD+GV+H DGYLEIKDRSKDVII GGEN+SS E+E+VLYTNP V EAAVVA+PD
Sbjct: 421 DGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKPD 480

Query: 481 EFWGETPCAFVSLR---EGLSRRP-TEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 540
           + WGETPCAFVSL+    G    P TE EI E+C+ KLP++MVP+ V+F+ ELPKTSTGK
Sbjct: 481 KMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTGK 540

Query: 541 IKKFVLREIAKSM 547
           I+KF+LR++AK++
Sbjct: 541 IQKFLLRQMAKTL 548

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022939278.11.7e-27784.20probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.... [more]
XP_022993747.11.9e-27683.60probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima][more]
XP_023550909.11.9e-27683.60probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo][more]
XP_038877068.14.4e-27382.62probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida][more]
XP_008463175.12.3e-26981.80PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA004... [more]
Match NameE-valueIdentityDescription
M4IQQ51.1e-24571.692-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1[more]
Q9FFE63.9e-23269.84Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FFE91.5e-22868.73Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 S... [more]
M4ISH23.0e-22468.59Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1[more]
Q9LQS11.9e-21867.34Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1FL768.3e-27884.20probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JTQ29.2e-27783.60probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5A7TXV01.1e-26981.80Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CIM61.1e-26981.80probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KT338.7e-26781.26AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G317890 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G16370.12.8e-23369.84acyl activating enzyme 5 [more]
AT5G16340.11.1e-22968.73AMP-dependent synthetase and ligase family protein [more]
AT1G75960.11.3e-21967.34AMP-dependent synthetase and ligase family protein [more]
AT1G21540.15.1e-21163.41AMP-dependent synthetase and ligase family protein [more]
AT1G21530.22.5e-21062.93AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 21..447
e-value: 7.9E-93
score: 311.3
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 3..442
e-value: 1.8E-99
score: 335.1
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 456..533
e-value: 1.0E-23
score: 84.1
NoneNo IPR availableGENE3D3.30.300.30coord: 443..552
e-value: 9.4E-30
score: 105.3
NoneNo IPR availablePANTHERPTHR43859:SF50LONG-CHAIN ACYL-COA SYNTHETASEcoord: 2..549
NoneNo IPR availablePANTHERPTHR43859ACYL-ACTIVATING ENZYMEcoord: 2..549
NoneNo IPR availableCDDcd12118ttLC_FACS_AEE21_likecoord: 12..541
e-value: 0.0
score: 828.093
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 11..545
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 191..202

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g09320.1Moc05g09320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016874 ligase activity