Homology
BLAST of Moc05g09320 vs. NCBI nr
Match:
XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 965.7 bits (2495), Expect = 1.7e-277
Identity = 469/557 (84.20%), Postives = 512/557 (91.92%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
I RG VVSVV+PNT AMYEL FAVPMAGAVLN INLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61 IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPV---SGDFVDTYEGMVEKGNPEF 180
SC LISDALALFPPE +RP L+LIAD+AAAE+SSS+ DFVD+YE MV+KG+P F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180
Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
+WIRPAS+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240
Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
HANGW FPWGIAA+GGTNVC+RKFDA IF LIHR++VTH+CGAPVVLNMLTNSP N+PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300
Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK W RLPAAERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360
Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
LKARQGVRT+A+ ADV+DPETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K+MT
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420
Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
EFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
Query: 541 VLREIAKSMGPARASRL 555
VLRE+AKSMGPA SRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557
BLAST of Moc05g09320 vs. NCBI nr
Match:
XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])
HSP 1 Score: 962.2 bits (2486), Expect = 1.9e-276
Identity = 469/561 (83.60%), Postives = 511/561 (91.09%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
I RG VVSVV+PNTPAMYEL FAVPMAGAVLN INLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61 IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS-------GDFVDTYEGMVEKG 180
SC LISDALALFPPE +RP L+LIAD+AAAE+SSSS S DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180
Query: 181 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 240
+P F+WIRP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240
Query: 241 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 300
LP+FHANGW FPWGIAA+GGTNVC+RKFDA IF LIHR++VTH+CGAPVVLNMLTNSP
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300
Query: 301 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 360
N+PLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK W RLPAA
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360
Query: 361 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 420
ERARLKARQGVRT+A+ ADV+ PETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420
Query: 421 SMTEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
+MT EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
Query: 481 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 540
ARPDEFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540
Query: 541 IKKFVLREIAKSMGPARASRL 555
I+KFVLRE+AKSMGPA SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561
BLAST of Moc05g09320 vs. NCBI nr
Match:
XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])
HSP 1 Score: 962.2 bits (2486), Expect = 1.9e-276
Identity = 469/561 (83.60%), Postives = 512/561 (91.27%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
MEDLKP+PANSSPLTPIGFL+RAA VYGDC S+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
I RG VVSVV+PNT AMYEL FAVPMAGAVLN INLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61 IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS-------GDFVDTYEGMVEKG 180
SC LISDALALFPPE +RP L+LIAD+AAAE+SSSS S DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180
Query: 181 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 240
+P F+WIRPAS+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240
Query: 241 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 300
LP+FHANGW FPWGIAA+GGTNVC+RKFDA IF LIHR++VTH+CGAPVVLNMLTNSP
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300
Query: 301 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 360
N+PLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK W RLPAA
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360
Query: 361 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 420
+RARLKARQGVRT+A+ ADV+DPETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420
Query: 421 SMTEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
+MT EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
Query: 481 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 540
ARPDEFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540
Query: 541 IKKFVLREIAKSMGPARASRL 555
I+KFVLRE+AKSMGPA SRL
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561
BLAST of Moc05g09320 vs. NCBI nr
Match:
XP_038877068.1 (probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida])
HSP 1 Score: 951.0 bits (2457), Expect = 4.4e-273
Identity = 461/558 (82.62%), Postives = 510/558 (91.40%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
MEDLKPS ANSSPLTPIGFL+RA+ +YGD PS+IY ST YTWS+T+RRCL++ASSLSS G
Sbjct: 28 MEDLKPSLANSSPLTPIGFLERASVIYGDSPSIIYNSTTYTWSETYRRCLRVASSLSSIG 87
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
I +G+VVSV+APNTP MYELQFAVPMAGAVLN INLRLDARTISILLRH ESKL+F+DQA
Sbjct: 88 IQKGYVVSVIAPNTPPMYELQFAVPMAGAVLNCINLRLDARTISILLRHCESKLIFIDQA 147
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEE----SSSSPVSGDFVDTYEGMVEKGNPE 180
SC LISDALALFPPE +RP LVLIADD A E+ SSSS + G+FVD+YE MV+KG+ E
Sbjct: 148 SCGLISDALALFPPETKRPLLVLIADDTAVEDSLSSSSSSVIGGEFVDSYEEMVQKGDDE 207
Query: 181 FRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPI 240
FRWIRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPKQSVYLWTLP+
Sbjct: 208 FRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFAVTLDSLIEWGVPKQSVYLWTLPM 267
Query: 241 FHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKP 300
FHANGW FP+GIAA+GGTNVC+RKFDA LIF LIHRH+VTH+CGAPVVLNMLTNSP N+P
Sbjct: 268 FHANGWSFPFGIAAVGGTNVCIRKFDAALIFSLIHRHHVTHLCGAPVVLNMLTNSPDNQP 327
Query: 301 LDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERA 360
L+RPVKILTAGAPPPAAVLFRTESLGFDV HGYGLTET GLV+ CSWK +W RLPA ERA
Sbjct: 328 LERPVKILTAGAPPPAAVLFRTESLGFDVGHGYGLTETGGLVIYCSWKSKWNRLPATERA 387
Query: 361 RLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMT 420
RLKARQGVRT+A++E DV+D ETGKSVKRDG+SIGEIVL+GGS+MLGY KDPEAT K+MT
Sbjct: 388 RLKARQGVRTLAMSEVDVLDSETGKSVKRDGASIGEIVLRGGSMMLGYFKDPEATKKAMT 447
Query: 421 EEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 480
+EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP
Sbjct: 448 DEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 507
Query: 481 DEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKK 540
DEFWGETPCAFVSLREGL+R PTEEEI+EYCR KLP+FMVPKTVVF +LPKTSTGKI+K
Sbjct: 508 DEFWGETPCAFVSLREGLTRLPTEEEIMEYCREKLPKFMVPKTVVFMEKLPKTSTGKIQK 567
Query: 541 FVLREIAKSMGPARASRL 555
F+LRE AKSMGPAR SRL
Sbjct: 568 FLLREKAKSMGPARTSRL 585
BLAST of Moc05g09320 vs. NCBI nr
Match:
XP_008463175.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046215.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18981.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])
HSP 1 Score: 938.7 bits (2425), Expect = 2.3e-269
Identity = 454/555 (81.80%), Postives = 508/555 (91.53%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
M+ LKP+PANSSPLTPI FL+RA+ VY D PS+IY ST++TWSQT+ RCL+LASSLSS G
Sbjct: 1 MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
IH+GHVVSV++PNTP MYELQF+VPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61 IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 180
S +LISDAL LFPPE++RP LVLIADDAA E+S+SS + GDFVD YE M++KG+PEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180
Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240
Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 300
NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTH+CGAPVVLNMLTNSP N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300
Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 360
PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360
Query: 361 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEG 420
ARQGVRT+A+ EADV+D ETGKSVKRDG SIGEIVL+GGS+MLGY KDPEAT K+MTEEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420
Query: 421 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
Query: 481 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 540
WGETPCAFVSLREGLSR PTEEEIIEYCRGKL FMVPKTVVF +LPKTSTGKI+KF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540
Query: 541 REIAKSMGPARASRL 555
RE+AKSMGP+R SRL
Sbjct: 541 REMAKSMGPSRKSRL 555
BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match:
M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)
HSP 1 Score: 850.5 bits (2196), Expect = 1.1e-245
Identity = 395/551 (71.69%), Postives = 483/551 (87.66%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
MEDLKP PA+SSPLTP+GFL+RAATVYGDC SV+Y + +YTWSQTHRRCL LASS++S G
Sbjct: 1 MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
I GHVVSV+APN P MYEL FAVPMAGA+LN +NLRLDARTISILL HSESKL+FVD
Sbjct: 61 IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRWI 180
S LI +A+ALFP +A P LV +AD+ +E +SS + +F +Y+ ++++G+P+F+W+
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADE--SESGNSSELGKEFFCSYKDLIDRGDPDFKWV 180
Query: 181 RPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHAN 240
P S+WDP++LNYTSGTTSSPKGVVHCHRG+F++ +DSL++WGVPKQ VYLWTLP+FHAN
Sbjct: 181 MPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHAN 240
Query: 241 GWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDRP 300
GW +PWG+AA+GGTN+CLRKFD+ +I+ +I RH VTHMCGAPVVLNML+N+P ++PL
Sbjct: 241 GWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKTT 300
Query: 301 VKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLKA 360
V+I+TAGAPPP+AVLFRTESLGF VSHGYGLTETAGLVV C+WK +W LPA ERARLK+
Sbjct: 301 VQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARLKS 360
Query: 361 RQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEGW 420
RQGV T+ T+ DVVDP TG +VKRDGS++GE+VL+GGS+MLGYLKDPE T+KSMT +GW
Sbjct: 361 RQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTADGW 420
Query: 421 FFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEFW 480
F+TGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVES+LY++P + EAAVVARPDEFW
Sbjct: 421 FYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDEFW 480
Query: 481 GETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVLR 540
GETPCAFVSL++GL+++PTE+EI+EYCR KLPR+MVPKTVVFK ELPKTSTGK++KF+LR
Sbjct: 481 GETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFILR 540
Query: 541 EIAKSMGPARA 552
++A+ MG A A
Sbjct: 541 DMARGMGSATA 549
BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match:
Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)
HSP 1 Score: 805.4 bits (2079), Expect = 3.9e-232
Identity = 389/557 (69.84%), Postives = 468/557 (84.02%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 60
ME +KP ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS
Sbjct: 1 MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60
Query: 61 GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
GI R VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH SKL+FVD
Sbjct: 61 GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120
Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSG--DFVDTYEGMVEKGNPEF 180
S L +A+++ + P LV IAD EE + V+ F TY+ ++ +G+ +F
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180
Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
+WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240
Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
HANGW +PWGIAA+GGTNVCLRKFDAPLI+RLI H VTHMCGAPVVLNML+ + + +PL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300
Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
+RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTETAGL V C+WKPQW RLPA++RAR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360
Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
LKARQGVRT+ TE DVVDPE+G+SV+R+G ++GEIV++G SIMLGYLKDP T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-K 420
Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480
Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
FWGETPCAFVSL+ GL++RPTE E+IEYCR K+P++MVPKTV F ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540
Query: 541 VLREIAKSMGPARASRL 555
VLREIAK MG R SR+
Sbjct: 541 VLREIAKKMGTTRLSRM 552
BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match:
Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)
HSP 1 Score: 793.5 bits (2048), Expect = 1.5e-228
Identity = 378/550 (68.73%), Postives = 464/550 (84.36%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 60
ME++KP ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS
Sbjct: 1 MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60
Query: 61 GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
GI R VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD
Sbjct: 61 GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120
Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS--GDFVDTYEGMVEKGNPEF 180
S L +A+++ + P LV+IAD EE + V+ F TY+ ++E+G+P F
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180
Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
+WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240
Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
H+NGW PWGIAA+GGTNVCLRKFDAPLI+RLI H VTHMCGAPVVLNML+ + +++PL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300
Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
+ PV ILTAG+PPPA VL R ES+GF +SHGYGLTETAG++V C+WKP+W LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360
Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
LKARQGVRT+ TE DVVDPE+G SV+R+G ++GEIV++G S+MLGYLKDP T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-K 420
Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480
Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
EFWGETPCAFVSL+ G S +PTEEE++EYCR K+P++MVPKTV F ELPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540
Query: 541 VLREIAKSMG 548
VLR+IAK MG
Sbjct: 541 VLRDIAKKMG 545
BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match:
M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)
HSP 1 Score: 779.2 bits (2011), Expect = 3.0e-224
Identity = 380/554 (68.59%), Postives = 453/554 (81.77%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSS-N 60
ME+LKP ANS PLTP+GFL+RAATVYGDC SV+Y +YTWSQTH RCL++AS + S
Sbjct: 1 MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60
Query: 61 GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
G+ +G VVSVVAPN PAMYEL FAVPMAG VLNNIN RL+A TIS++LRHSESKLVFVDQ
Sbjct: 61 GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120
Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPE-FR 180
S L+ DA++LFP P LVLIAD+ E + FV YE +VEKG+ + F+
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGENLTVED-REHFVCCYEDLVEKGDDKTFK 180
Query: 181 WIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFH 240
W+RP S+WDPIVLNYTSGTTSSPKGVVH HR VF+V LDSL++WGVPKQ VYLWTLP+FH
Sbjct: 181 WVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMFH 240
Query: 241 ANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLD 300
ANGW + WGIAA+GGTN+CLR+FD +IF LI RH VTHMC AP+VLNML+NSP +PL
Sbjct: 241 ANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPLP 300
Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARL 360
PV ++T GAPPPAAVL RTESLGF +SHGYG+TE G+VV C+WK +W RLPA E+ARL
Sbjct: 301 NPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQARL 360
Query: 361 KARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEE 420
K+RQGVRT A+ E DVVDP +G SVKRDG ++GEIVLKG SIMLGYLK+ AT+K + +
Sbjct: 361 KSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIRAD 420
Query: 421 GWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
GWF+TGD+ VMH DGYLEIKDRSKDVIISGGEN+SSVEVES LY++PAV+EAAVVA PDE
Sbjct: 421 GWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACPDE 480
Query: 481 FWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFV 540
+WGETP AFV+L++G+ RPTE+EI+EYCR KL FMVPK VVF+ +LPKTSTGKI+KF
Sbjct: 481 YWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQKFT 540
Query: 541 LREIAKSMGPARAS 553
L+EI K+MG + +S
Sbjct: 541 LKEIVKTMGYSSSS 553
BLAST of Moc05g09320 vs. ExPASy Swiss-Prot
Match:
Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)
HSP 1 Score: 760.0 bits (1961), Expect = 1.9e-218
Identity = 369/548 (67.34%), Postives = 446/548 (81.39%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYG-STAYTWSQTHRRCLQLASSLSSN 60
MEDLKPS ANS PLT +GFL+RAATVYGDC S++YG ST YTW +T+ RCL +AS+LSS
Sbjct: 1 MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60
Query: 61 GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
GI R VVSV++ NTP MYELQF+VPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD
Sbjct: 61 GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120
Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRW 180
L +A+ + P LVLIA++ E + F Y ++ +GNP+F+W
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFKW 180
Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
IRP S+WDPIV+NYTSGTTSSPKGVVHCHRG+F++ LDSL +W VPK VYLWTLPIFHA
Sbjct: 181 IRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFHA 240
Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK-NKPLD 300
NGW +PWGIAA+GGTNVC+RK AP I+ LI H VTHM GAP+VL +L+ S + ++PL
Sbjct: 241 NGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPLK 300
Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARL 360
PV LTAG+ PPA VL R ESLGF VSHGYGLTETAG++V C+WKP W RLPA+++A+L
Sbjct: 301 SPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQL 360
Query: 361 KARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEE 420
K+RQGVRT+ +E DVVDPE+G+SV+RDG ++GEIVL+G SIMLGYLK+P T S +
Sbjct: 361 KSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-KN 420
Query: 421 GWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
GWFFTGD+GV+HGDGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPDE
Sbjct: 421 GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPDE 480
Query: 481 FWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFV 540
FWGETPCAFVSL+ GL+R+PT++EIIEYC+ K+PR+M PKTV F ELPKTSTGKI K +
Sbjct: 481 FWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKSL 540
Query: 541 LREIAKSM 547
L+EIAK+M
Sbjct: 541 LKEIAKNM 544
BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match:
A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)
HSP 1 Score: 965.7 bits (2495), Expect = 8.3e-278
Identity = 469/557 (84.20%), Postives = 512/557 (91.92%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
I RG VVSVV+PNT AMYEL FAVPMAGAVLN INLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61 IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPV---SGDFVDTYEGMVEKGNPEF 180
SC LISDALALFPPE +RP L+LIAD+AAAE+SSS+ DFVD+YE MV+KG+P F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180
Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
+WIRPAS+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240
Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
HANGW FPWGIAA+GGTNVC+RKFDA IF LIHR++VTH+CGAPVVLNMLTNSP N+PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300
Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK W RLPAAERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360
Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
LKARQGVRT+A+ ADV+DPETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K+MT
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420
Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
EFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGKI+KF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
Query: 541 VLREIAKSMGPARASRL 555
VLRE+AKSMGPA SRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557
BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match:
A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)
HSP 1 Score: 962.2 bits (2486), Expect = 9.2e-277
Identity = 469/561 (83.60%), Postives = 511/561 (91.09%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
MEDLKP+PANSSPLTPIGFL+RAA VYGDCPS+IY ST YTW++THRRCLQLASSL S G
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
I RG VVSVV+PNTPAMYEL FAVPMAGAVLN INLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61 IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS-------GDFVDTYEGMVEKG 180
SC LISDALALFPPE +RP L+LIAD+AAAE+SSSS S DFVD+YE MV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180
Query: 181 NPEFRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWT 240
+P F+WIRP S+WDPIVLNYTSGTTSSPKGVVHCHRG+F V LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240
Query: 241 LPIFHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK 300
LP+FHANGW FPWGIAA+GGTNVC+RKFDA IF LIHR++VTH+CGAPVVLNMLTNSP
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300
Query: 301 NKPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAA 360
N+PLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK W RLPAA
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360
Query: 361 ERARLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSK 420
ERARLKARQGVRT+A+ ADV+ PETGKSVKRDG+SIGEIVL+G S+MLGYLKDPEAT+K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420
Query: 421 SMTEEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
+MT EGWFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
Query: 481 ARPDEFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 540
ARPDEFWGETPCAFVSL+EGL+RRPTE EIIEYCRGKLPRFMVPKTVVF AELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540
Query: 541 IKKFVLREIAKSMGPARASRL 555
I+KFVLRE+AKSMGPA SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561
BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match:
A0A5A7TXV0 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00770 PE=4 SV=1)
HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 454/555 (81.80%), Postives = 508/555 (91.53%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
M+ LKP+PANSSPLTPI FL+RA+ VY D PS+IY ST++TWSQT+ RCL+LASSLSS G
Sbjct: 1 MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
IH+GHVVSV++PNTP MYELQF+VPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61 IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 180
S +LISDAL LFPPE++RP LVLIADDAA E+S+SS + GDFVD YE M++KG+PEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180
Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240
Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 300
NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTH+CGAPVVLNMLTNSP N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300
Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 360
PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360
Query: 361 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEG 420
ARQGVRT+A+ EADV+D ETGKSVKRDG SIGEIVL+GGS+MLGY KDPEAT K+MTEEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420
Query: 421 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
Query: 481 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 540
WGETPCAFVSLREGLSR PTEEEIIEYCRGKL FMVPKTVVF +LPKTSTGKI+KF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540
Query: 541 REIAKSMGPARASRL 555
RE+AKSMGP+R SRL
Sbjct: 541 REMAKSMGPSRKSRL 555
BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match:
A0A1S3CIM6 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501383 PE=4 SV=1)
HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 454/555 (81.80%), Postives = 508/555 (91.53%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
M+ LKP+PANSSPLTPI FL+RA+ VY D PS+IY ST++TWSQT+ RCL+LASSLSS G
Sbjct: 1 MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
IH+GHVVSV++PNTP MYELQF+VPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 61 IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 180
S +LISDAL LFPPE++RP LVLIADDAA E+S+SS + GDFVD YE M++KG+PEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180
Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240
Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 300
NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTH+CGAPVVLNMLTNSP N+PL+R
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300
Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 360
PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360
Query: 361 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEG 420
ARQGVRT+A+ EADV+D ETGKSVKRDG SIGEIVL+GGS+MLGY KDPEAT K+MTEEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420
Query: 421 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
Query: 481 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 540
WGETPCAFVSLREGLSR PTEEEIIEYCRGKL FMVPKTVVF +LPKTSTGKI+KF+L
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540
Query: 541 REIAKSMGPARASRL 555
RE+AKSMGP+R SRL
Sbjct: 541 REMAKSMGPSRKSRL 555
BLAST of Moc05g09320 vs. ExPASy TrEMBL
Match:
A0A0A0KT33 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G317890 PE=4 SV=1)
HSP 1 Score: 929.1 bits (2400), Expect = 8.7e-267
Identity = 451/555 (81.26%), Postives = 503/555 (90.63%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGSTAYTWSQTHRRCLQLASSLSSNG 60
ME LKP+PANSSPLTPIGFL+RA+ +Y D PS+IY ST +TWSQT+RRCL+LASSLSS G
Sbjct: 22 MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81
Query: 61 IHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQA 120
IH+GHVVSV+APNTP MYELQFAVPMAGA+L+ INLRLDARTIS+ LRHSESKL+FVDQA
Sbjct: 82 IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141
Query: 121 SCSLISDALALFPPEARRPALVLIADDAAAEESSSS-PVSGDFVDTYEGMVEKGNPEFRW 180
S LISDALALFPPEA+RP LVLI+DDAA E+S+SS + GD VD YE MV+KG+PEFRW
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFRW 201
Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
IRP S+WDPIV+NYTSGTTSSPKGVVH HRG+F V LDSL+EWGVPK SVYLWTLP+FHA
Sbjct: 202 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 261
Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPLDR 300
NGW FP+ IAA+GGTN+C+RKFDA LIF LI+RH+VTHMCGAPVVLNMLTNSP N+PL+R
Sbjct: 262 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLER 321
Query: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARLK 360
PVKILTAGAPPPAAVL RTESLGFDVSHGYGLTET GLV+ CSWK +W +LPA ERARLK
Sbjct: 322 PVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARLK 381
Query: 361 ARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEEG 420
ARQGVRT+A+ EADV+D TGK V+RDG SIGEIV++GGS+MLGY KDPEAT K+MTEEG
Sbjct: 382 ARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEEG 441
Query: 421 WFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480
WFFTGDVGVMH DGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 442 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501
Query: 481 WGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFVL 540
WGETPCAFVSLREGL+R PTEEEIIEYC+GKL FMVPKTVVF +LPKTSTGKI+KF+L
Sbjct: 502 WGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 561
Query: 541 REIAKSMGPARASRL 555
RE+AKSMG + SRL
Sbjct: 562 REMAKSMGCSPKSRL 576
BLAST of Moc05g09320 vs. TAIR 10
Match:
AT5G16370.1 (acyl activating enzyme 5 )
HSP 1 Score: 805.4 bits (2079), Expect = 2.8e-233
Identity = 389/557 (69.84%), Postives = 468/557 (84.02%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 60
ME +KP ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS
Sbjct: 1 MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60
Query: 61 GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
GI R VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH SKL+FVD
Sbjct: 61 GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120
Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSG--DFVDTYEGMVEKGNPEF 180
S L +A+++ + P LV IAD EE + V+ F TY+ ++ +G+ +F
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180
Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
+WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240
Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
HANGW +PWGIAA+GGTNVCLRKFDAPLI+RLI H VTHMCGAPVVLNML+ + + +PL
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300
Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
+RPV ILTAGAPPPAAVL R ES+GF +SHGYGLTETAGL V C+WKPQW RLPA++RAR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360
Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
LKARQGVRT+ TE DVVDPE+G+SV+R+G ++GEIV++G SIMLGYLKDP T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-K 420
Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480
Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
FWGETPCAFVSL+ GL++RPTE E+IEYCR K+P++MVPKTV F ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540
Query: 541 VLREIAKSMGPARASRL 555
VLREIAK MG R SR+
Sbjct: 541 VLREIAKKMGTTRLSRM 552
BLAST of Moc05g09320 vs. TAIR 10
Match:
AT5G16340.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 793.5 bits (2048), Expect = 1.1e-229
Identity = 378/550 (68.73%), Postives = 464/550 (84.36%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYGS-TAYTWSQTHRRCLQLASSLSSN 60
ME++KP ANS PLTPIGFL+RAATVYGDC S++YGS T YTW +T+ RCL++ASSLSS
Sbjct: 1 MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60
Query: 61 GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
GI R VVSV++PNTPAMYELQFAVPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD
Sbjct: 61 GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120
Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVS--GDFVDTYEGMVEKGNPEF 180
S L +A+++ + P LV+IAD EE + V+ F TY+ ++E+G+P F
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180
Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
+WIRP S+WDP+VLNYTSGTTS+PKGVVHCHRG+F++++DSL++W VPK VYLWTLPIF
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240
Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
H+NGW PWGIAA+GGTNVCLRKFDAPLI+RLI H VTHMCGAPVVLNML+ + +++PL
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300
Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
+ PV ILTAG+PPPA VL R ES+GF +SHGYGLTETAG++V C+WKP+W LPA++RAR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360
Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
LKARQGVRT+ TE DVVDPE+G SV+R+G ++GEIV++G S+MLGYLKDP T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-K 420
Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AVVARPD
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480
Query: 481 EFWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKF 540
EFWGETPCAFVSL+ G S +PTEEE++EYCR K+P++MVPKTV F ELPK+STGK+ KF
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540
Query: 541 VLREIAKSMG 548
VLR+IAK MG
Sbjct: 541 VLRDIAKKMG 545
BLAST of Moc05g09320 vs. TAIR 10
Match:
AT1G75960.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 760.0 bits (1961), Expect = 1.3e-219
Identity = 369/548 (67.34%), Postives = 446/548 (81.39%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIYG-STAYTWSQTHRRCLQLASSLSSN 60
MEDLKPS ANS PLT +GFL+RAATVYGDC S++YG ST YTW +T+ RCL +AS+LSS
Sbjct: 1 MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60
Query: 61 GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFVDQ 120
GI R VVSV++ NTP MYELQF+VPM+GA+LNNIN RLDART+S+LLRH ESKL+FVD
Sbjct: 61 GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120
Query: 121 ASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEFRW 180
L +A+ + P LVLIA++ E + F Y ++ +GNP+F+W
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFKW 180
Query: 181 IRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIFHA 240
IRP S+WDPIV+NYTSGTTSSPKGVVHCHRG+F++ LDSL +W VPK VYLWTLPIFHA
Sbjct: 181 IRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFHA 240
Query: 241 NGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPK-NKPLD 300
NGW +PWGIAA+GGTNVC+RK AP I+ LI H VTHM GAP+VL +L+ S + ++PL
Sbjct: 241 NGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPLK 300
Query: 301 RPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERARL 360
PV LTAG+ PPA VL R ESLGF VSHGYGLTETAG++V C+WKP W RLPA+++A+L
Sbjct: 301 SPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQL 360
Query: 361 KARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTEE 420
K+RQGVRT+ +E DVVDPE+G+SV+RDG ++GEIVL+G SIMLGYLK+P T S +
Sbjct: 361 KSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-KN 420
Query: 421 GWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480
GWFFTGD+GV+HGDGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPDE
Sbjct: 421 GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPDE 480
Query: 481 FWGETPCAFVSLREGLSRRPTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKIKKFV 540
FWGETPCAFVSL+ GL+R+PT++EIIEYC+ K+PR+M PKTV F ELPKTSTGKI K +
Sbjct: 481 FWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKSL 540
Query: 541 LREIAKSM 547
L+EIAK+M
Sbjct: 541 LKEIAKNM 544
BLAST of Moc05g09320 vs. TAIR 10
Match:
AT1G21540.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 731.5 bits (1887), Expect = 5.1e-211
Identity = 350/552 (63.41%), Postives = 445/552 (80.62%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIY-GSTAYTWSQTHRRCLQLASSLSSN 60
ME L P P+NS+PLT +GFLDRAA+VYGDCPS+++ +T +TWS+TH RCL++AS+L+S+
Sbjct: 1 MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60
Query: 61 --GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFV 120
GI+RG VVSVV PN P++YELQFAVPM+GA+LNNIN RLDA +S+LLRHSESKLVFV
Sbjct: 61 SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120
Query: 121 DQASCSLISDALALFPPEARRPALVLIADDAAAEESSSSP-VSGDFVDTYEGMVEKGNPE 180
D S S++ +A++ F + +P LVL+ DD E+ S SP + DF+DTY+G++E+G+
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAASDFLDTYQGVMERGDSR 180
Query: 181 FRWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPI 240
F+WIRP ++W P++LNYTSGTTSSPKGVV HR +FM+ + SLL+W P + VYLWTLP+
Sbjct: 181 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 240
Query: 241 FHANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKP 300
FHANGW + WG AA+G TNVC R+ DAP I+ LI +H+VTHMC AP+VLNMLTN P KP
Sbjct: 241 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 300
Query: 301 LDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERA 360
L PV+++TAGAPPPAA++ R E+LGF+V HGYGLTET G VV C+WK +W L ERA
Sbjct: 301 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 360
Query: 361 RLKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMT 420
RLK+RQGVRT+ E DV DP TGKSV+ DG S+GEIVLKGGS+MLGY KDPE T+ M
Sbjct: 361 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 420
Query: 421 EEGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 480
E+GWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLYTNP V EAAVVA+P
Sbjct: 421 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 480
Query: 481 DEFWGETPCAFVSLREGLSRR--PTEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGKI 540
D+ WGETPCAFVSL+ + TE EI E+C+ +LP++MVP+ V+F+ ELPKTSTGKI
Sbjct: 481 DKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGKI 540
Query: 541 KKFVLREIAKSM 547
+KF+LR++AKS+
Sbjct: 541 QKFLLRQMAKSL 549
BLAST of Moc05g09320 vs. TAIR 10
Match:
AT1G21530.2 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 729.2 bits (1881), Expect = 2.5e-210
Identity = 348/553 (62.93%), Postives = 444/553 (80.29%), Query Frame = 0
Query: 1 MEDLKPSPANSSPLTPIGFLDRAATVYGDCPSVIY-GSTAYTWSQTHRRCLQLASSLSSN 60
ME L P P+NS+PLT +GFLDRAA+VYGDCPS+++ +T +TWS+TH RCL++AS+L+S+
Sbjct: 1 MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60
Query: 61 --GIHRGHVVSVVAPNTPAMYELQFAVPMAGAVLNNINLRLDARTISILLRHSESKLVFV 120
GI +G VVSVV PN P++YELQFAVPM+GA+LNNIN RLDA +S+LLRHSES+LVFV
Sbjct: 61 SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120
Query: 121 DQASCSLISDALALFPPEARRPALVLIADDAAAEESSSSPVSGDFVDTYEGMVEKGNPEF 180
D S SL+ +A++LF + +P LVL+ DD + SS+S DF+DTYE ++E+GN F
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLLDDDQENDSSSAS----DFLDTYEEIMERGNSRF 180
Query: 181 RWIRPASDWDPIVLNYTSGTTSSPKGVVHCHRGVFMVALDSLLEWGVPKQSVYLWTLPIF 240
+WIRP ++W P+VLNYTSGTTSSPKGVV HR +FM+ + SLL+W VP + VYLWTLP+F
Sbjct: 181 KWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTLPMF 240
Query: 241 HANGWCFPWGIAAIGGTNVCLRKFDAPLIFRLIHRHNVTHMCGAPVVLNMLTNSPKNKPL 300
HANGW + WG AA+G TN+C R+ DAP I+ LI +HNVTHMC AP+VLNML N P + PL
Sbjct: 241 HANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLSTPL 300
Query: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETAGLVVVCSWKPQWKRLPAAERAR 360
PV+++T+GAPPPA ++ R ESLGF+VSH YGLTET+G VV C+WKP+W L ERAR
Sbjct: 301 KNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLERAR 360
Query: 361 LKARQGVRTMAITEADVVDPETGKSVKRDGSSIGEIVLKGGSIMLGYLKDPEATSKSMTE 420
LK+RQGVRT+ TE DV D +TGKSVK DG S+GEIV +G S+MLGY KDP+ T+ M E
Sbjct: 361 LKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAACMRE 420
Query: 421 EGWFFTGDVGVMHGDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
+GWF++GD+GV+H DGYLEIKDRSKDVII GGEN+SS E+E+VLYTNP V EAAVVA+PD
Sbjct: 421 DGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKPD 480
Query: 481 EFWGETPCAFVSLR---EGLSRRP-TEEEIIEYCRGKLPRFMVPKTVVFKAELPKTSTGK 540
+ WGETPCAFVSL+ G P TE EI E+C+ KLP++MVP+ V+F+ ELPKTSTGK
Sbjct: 481 KMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTGK 540
Query: 541 IKKFVLREIAKSM 547
I+KF+LR++AK++
Sbjct: 541 IQKFLLRQMAKTL 548
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022939278.1 | 1.7e-277 | 84.20 | probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.... | [more] |
XP_022993747.1 | 1.9e-276 | 83.60 | probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima] | [more] |
XP_023550909.1 | 1.9e-276 | 83.60 | probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo] | [more] |
XP_038877068.1 | 4.4e-273 | 82.62 | probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida] | [more] |
XP_008463175.1 | 2.3e-269 | 81.80 | PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA004... | [more] |
Match Name | E-value | Identity | Description | |
M4IQQ5 | 1.1e-245 | 71.69 | 2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1 | [more] |
Q9FFE6 | 3.9e-232 | 69.84 | Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 G... | [more] |
Q9FFE9 | 1.5e-228 | 68.73 | Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 S... | [more] |
M4ISH2 | 3.0e-224 | 68.59 | Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1 | [more] |
Q9LQS1 | 1.9e-218 | 67.34 | Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 S... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FL76 | 8.3e-278 | 84.20 | probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=... | [more] |
A0A6J1JTQ2 | 9.2e-277 | 83.60 | probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO... | [more] |
A0A5A7TXV0 | 1.1e-269 | 81.80 | Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=11... | [more] |
A0A1S3CIM6 | 1.1e-269 | 81.80 | probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103... | [more] |
A0A0A0KT33 | 8.7e-267 | 81.26 | AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G317890 PE=4 SV=1 | [more] |