Moc05g07640 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g07640
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionProtein-serine/threonine kinase
Locationchr5: 5601055 .. 5604056 (+)
RNA-Seq ExpressionMoc05g07640
SyntenyMoc05g07640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTAAGAAACTCTGCGAGACCTTCCCCAAGAGCCTGATCGACGAGGTCCAGAAATGGGGTTGTATGAAACAGACGGGGGTCAGTTTGCGATACATGATGGAGTTCGGGTCGAAGCCGACTGAGAAGAATTTGCTCATTTCTGCCCAGTTTCTTCACAAGGAGCTTCCCATTCGGATTGCCAGAAGAGCTATTGAACTCGAGAATCTTCCCTATGGCCTCTCTGCGAAACCTGCTGTTTTGAAGGTTGGTTTTCACCCCTTTTATCTGCTTTGCATTTCCCTGAATTGGTTGTGTTTTCTTTTCTTTTTTTTTTTTGAAATCTCGAATGTATGAGGAATTTCTGGCAGTGGGCAATTTCGAATATCTTTAATGGATATAATGTTTCTTGTGTCTGGCAGAGAAGGTTTCATCATTTCACTTCATTGGCTTCTGATTGTGGTTTCTGATCATCACTTTTTTCAGCCCCTTTCTCTGTTGGAAATTTACTTGTTTGTTTCAGATTTCCAATTTTGGATCCTAATCTTTTCATGCTGGATTTATTTATTCATTAAGGGCAGTTGGCAAAATTCTGTGCAGTTATTTAATTCTATTCATAGCTATTGTAGAAGATTCCTTTTTGACTTGAACATCCTAAAGTGCTTTTGCTGGTTAACAGTTTTCTGCTGGTGATTGCGTTTTGCTTGCTTTTAAGAGTTCGAATCTTTGTTGTATTTTATATGAAGGCTATATTTAAGACATATTTTGCCTCAGGAAAGTACTTGCATGCATAATGAAATGCTTGGATGTAGTAGGGATTTGTTTGGGAAATCTTTTCAGAAAAGTTTCTGCTGTTTGGAATGAATTTCTGGAACAATGTTTGTTGGCAATTGTTTCTTAAAGTTGTTCTAGAAACTGCCTGTGTTTCAGAAAAATGAAGTTATTAGTCATGTCAAAGTCACATGCTTGTGAATAGTGATCTCAATGTTCTCAATAATATGAACTGTTACAGATTATTTAATCACAATCTTCAAGAACGATTTTGAAATTTGAAAGCAGAAAACTGTTTTTGAAACAGTTTCCCAAACTTGGCCTATTAGTTTCTTATTTGAGTTTCACAGTGCATGTAAAGGCAGGATTCTATCTAAGTAATTTTTAGTACTACCATTTACTTTTGAAGTTGTGTTTCAGTTTTCCAGGGATACTGCGTTTACTTTTTTTTTTTTTTTTCTTTAGGTTCGTGACTGGTACTTGGATTCTTTCCGTGACCTTCGGTCTTTTCCCGAGATAAAGAGTTCAGATGATGAACAAGAGTTCACTCAAATGATAAAGGCAATCAAGGTGAGACATAATAATGTGGTTCCAACGATGGCTTTAGGTGTTCAACAACTGAAGAAAAGCCTGGGTCCAAGCAATATTGGGCATCAAGATCTTCGGGAGATTCATCAGTTTTTGGACCGTTTTTACATGTCAAGAATTGGGATTCGTATGCTCATTGGTTAGTTGAATTTCATGTGCCTTAGTCAGAAGTCCCTGAGTCTCGATCGTTTCTGTCTTCCCCACTTCCTTCTCTATTCTGTCTATGTACTTTTAAGTTCTCTTGAAGTGGGGTATGGGAATATTCAAATCTTACTATTCTTTTATGTTTTTCAAAGGCTTAATACGTCGTTGACAAAATTCGATTGTAACTCAGGGCAGCACGTGGAATTGCACAATCCCAATCCCCCTCCTGACTGTGTAGGATATATACATACAAAAATGTCACCTATGGAGGTAGCACAGAGTGCCAGTGAGGATGCTCGTGCGATCTGTTTACGTGAGTATGGCAGTGCCCCTAATATCAAAATCTATGGAGATCCTAATTTTACATTTCCGTAAGTTCTTCGCTCTGACTCCATGTGTTGCTTACGATAAACGCTTCAGCTTTAAACTTTATTCACTCTGTTCTGAGTGTTTCTGATCTATGCCAATTTCTCTGAGTGTGGGTGACTTGTGGCCTTGTGGCATGCATGATGTTGGGGCAACTGCTCTCTGTTCTGTTCTGGTCTTACATATTCAGTTCTTATATCGTTTACTTTTCCAGTTATGTTCCAACACACTTGCAACTTATGGTCTTTGAGTTGGTTAAGAACTCGTTACGTGCCGTGCAAGAGCGTTTCATGGATTCTGATAAAGTTTATCCTCCTGTTAGAATCATAGTTGCTGATGGAATTGAGGATGTCACCATTAAGGTACTGCTCTTGTGCATTCCCAAAGTTAAGGAACTTCATTTCTCTATGCAGTTTTCAAATAAGTGTGCCTTCTACTGACTTCGCTAGGTATCCGATGAGGGCGGTGGCATACCCCGAAGCGGCCTCCCCAAAATTTTCACTTACCTTTACACCACTGCCAGAAACCCGCTGGATGAACATCCGGATTTGGGTACAGCAGATTTGGTGACAATGGCTGGATATGGCTATGGCCTTCCAATAAGCCGTCTGTATGCTCGCTATTTTGGTGGGGATCTGCAAGTGATCTCCATGGAAGGATATGGTAAGTACATTCTGATTGTTTTAGTCATGTGAGTAGTAGTTTCCAAAATATTACTTTTAGTCATGTCGTCGTTTTTCGGTTCCTTCATTGATTGGTCTTTCCTCCCCAATTAGTATCTGTTGAAATCGAATCATGTACAGTTCTCACATCCATATCACTGATTGTCATTCATTCTACTTATTTGCATAATTAAATTGCCTTTGGTAGTTGGGGACGGGGACAGAGACAGGACGTTTTACTTTCTTTTAGACTCCATCAATTGCAGAGTTGATGGGAAGTATATCCACGAGTCTATGGTAGTAGTAGCTCAGCCGAATTCAAATGATAATTGTTGCCAACGTCTTGCTTCTCTGTGCAAATTCACGGTTGATCAATATGCTTGTTTTTTCGGGCCGATAACTAATGTTGTTATTGAACTACTGTAAACAGGAACAGATGCATATCTTCACCTGTCTCGTCTGGGGGATTCACAAGAGCCTCTGCCGTGA

mRNA sequence

ATGGCGGCTAAGAAACTCTGCGAGACCTTCCCCAAGAGCCTGATCGACGAGGTCCAGAAATGGGGTTGTATGAAACAGACGGGGGTCAGTTTGCGATACATGATGGAGTTCGGGTCGAAGCCGACTGAGAAGAATTTGCTCATTTCTGCCCAGTTTCTTCACAAGGAGCTTCCCATTCGGATTGCCAGAAGAGCTATTGAACTCGAGAATCTTCCCTATGGCCTCTCTGCGAAACCTGCTGTTTTGAAGGTTCGTGACTGGTACTTGGATTCTTTCCGTGACCTTCGGTCTTTTCCCGAGATAAAGAGTTCAGATGATGAACAAGAGTTCACTCAAATGATAAAGGCAATCAAGGTGAGACATAATAATGTGGTTCCAACGATGGCTTTAGGTGTTCAACAACTGAAGAAAAGCCTGGGTCCAAGCAATATTGGGCATCAAGATCTTCGGGAGATTCATCAGTTTTTGGACCGTTTTTACATGTCAAGAATTGGGATTCGTATGCTCATTGGGCAGCACGTGGAATTGCACAATCCCAATCCCCCTCCTGACTGTGTAGGATATATACATACAAAAATGTCACCTATGGAGGTAGCACAGAGTGCCAGTGAGGATGCTCGTGCGATCTGTTTACGTGAGTATGGCAGTGCCCCTAATATCAAAATCTATGGAGATCCTAATTTTACATTTCCTTATGTTCCAACACACTTGCAACTTATGGTCTTTGAGTTGGTTAAGAACTCGTTACGTGCCGTGCAAGAGCGTTTCATGGATTCTGATAAAGTTTATCCTCCTGTTAGAATCATAGTTGCTGATGGAATTGAGGATGTCACCATTAAGGTATCCGATGAGGGCGGTGGCATACCCCGAAGCGGCCTCCCCAAAATTTTCACTTACCTTTACACCACTGCCAGAAACCCGCTGGATGAACATCCGGATTTGGGTACAGCAGATTTGGTGACAATGGCTGGATATGGCTATGGCCTTCCAATAAGCCGTCTGTATGCTCGCTATTTTGGTGGGGATCTGCAAGTGATCTCCATGGAAGGATATGGAACAGATGCATATCTTCACCTGTCTCGTCTGGGGGATTCACAAGAGCCTCTGCCGTGA

Coding sequence (CDS)

ATGGCGGCTAAGAAACTCTGCGAGACCTTCCCCAAGAGCCTGATCGACGAGGTCCAGAAATGGGGTTGTATGAAACAGACGGGGGTCAGTTTGCGATACATGATGGAGTTCGGGTCGAAGCCGACTGAGAAGAATTTGCTCATTTCTGCCCAGTTTCTTCACAAGGAGCTTCCCATTCGGATTGCCAGAAGAGCTATTGAACTCGAGAATCTTCCCTATGGCCTCTCTGCGAAACCTGCTGTTTTGAAGGTTCGTGACTGGTACTTGGATTCTTTCCGTGACCTTCGGTCTTTTCCCGAGATAAAGAGTTCAGATGATGAACAAGAGTTCACTCAAATGATAAAGGCAATCAAGGTGAGACATAATAATGTGGTTCCAACGATGGCTTTAGGTGTTCAACAACTGAAGAAAAGCCTGGGTCCAAGCAATATTGGGCATCAAGATCTTCGGGAGATTCATCAGTTTTTGGACCGTTTTTACATGTCAAGAATTGGGATTCGTATGCTCATTGGGCAGCACGTGGAATTGCACAATCCCAATCCCCCTCCTGACTGTGTAGGATATATACATACAAAAATGTCACCTATGGAGGTAGCACAGAGTGCCAGTGAGGATGCTCGTGCGATCTGTTTACGTGAGTATGGCAGTGCCCCTAATATCAAAATCTATGGAGATCCTAATTTTACATTTCCTTATGTTCCAACACACTTGCAACTTATGGTCTTTGAGTTGGTTAAGAACTCGTTACGTGCCGTGCAAGAGCGTTTCATGGATTCTGATAAAGTTTATCCTCCTGTTAGAATCATAGTTGCTGATGGAATTGAGGATGTCACCATTAAGGTATCCGATGAGGGCGGTGGCATACCCCGAAGCGGCCTCCCCAAAATTTTCACTTACCTTTACACCACTGCCAGAAACCCGCTGGATGAACATCCGGATTTGGGTACAGCAGATTTGGTGACAATGGCTGGATATGGCTATGGCCTTCCAATAAGCCGTCTGTATGCTCGCTATTTTGGTGGGGATCTGCAAGTGATCTCCATGGAAGGATATGGAACAGATGCATATCTTCACCTGTCTCGTCTGGGGGATTCACAAGAGCCTCTGCCGTGA

Protein sequence

MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIRIARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVRHNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPNPPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLMVFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYLYTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLSRLGDSQEPLP
Homology
BLAST of Moc05g07640 vs. NCBI nr
Match: XP_022138237.1 (pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Momordica charantia])

HSP 1 Score: 759.6 bits (1960), Expect = 1.3e-215
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM
Sbjct: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. NCBI nr
Match: XP_004147906.3 (pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucumis sativus] >KAE8649559.1 hypothetical protein Csa_018139 [Cucumis sativus])

HSP 1 Score: 718.4 bits (1853), Expect = 3.2e-203
Identity = 348/370 (94.05%), Postives = 361/370 (97.57%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCE+F KSLID+VQKWGCMKQTGVSLRYMMEFGSKPT KNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCESFSKSLIDDVQKWGCMKQTGVSLRYMMEFGSKPTAKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLS KPAVLKVRDWYLDSFRDLRSFPEIK+SDDE+EFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYLDSFRDLRSFPEIKNSDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVPTMALGVQQLKK L  +N+G+Q+L EIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVQQLKKGLRLNNVGYQNLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPDCVGYIHTKMSP++VA+SASEDARAICLREYGSAPNIKIYGDP+FTFPYVPTHL LM
Sbjct: 181 PPPDCVGYIHTKMSPVKVAESASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQERFMDSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTA+NPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. NCBI nr
Match: XP_022979822.1 (pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 716.5 bits (1848), Expect = 1.2e-202
Identity = 347/370 (93.78%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCETF KSLIDEVQKWGCMKQTGVSLRYMMEFG KPT KNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGLKPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLS KPAVLKVRDWYLDSFRDLR+FPEIK+ DDE+EFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYLDSFRDLRAFPEIKNPDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVP MALGVQQLKKS+   NIG+QDL EIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPMMALGVQQLKKSIALDNIGYQDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPDCVGYIHTKMSP++VA+SASEDARAICLREYGSAPNIKIYGDP+FTFPYVPTHLQLM
Sbjct: 181 PPPDCVGYIHTKMSPVKVAESASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLQLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQERFMDSDKV PPV+IIVADGIEDVTIKVSDEGGGIPRSGLP+IFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVKIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTA+NPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. NCBI nr
Match: XP_038894097.1 (pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial-like isoform X1 [Benincasa hispida])

HSP 1 Score: 714.9 bits (1844), Expect = 3.5e-202
Identity = 349/370 (94.32%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCETF KSLIDEVQKWGCMKQTGVSLRYMMEFGSKPT KNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLS KPAVLKVRDWY+DSFRDLRSFPEIKSSDDE+EFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVPTMALGV+QLKK LG +NIG  DL EIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVRQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPD VGYIHTKMSP+EVAQSASEDARAICLREYGSAPNIKIYGDP+FTFPYVPTHL LM
Sbjct: 181 PPPDRVGYIHTKMSPVEVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQERFMDSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLP+IFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTA++PLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQ+ISMEGYGTDAYLHLS
Sbjct: 301 YTTAKDPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQMISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. NCBI nr
Match: XP_023528572.1 (pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucurbita pepo subsp. pepo] >KAG6582145.1 [Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018544.1 [Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 714.5 bits (1843), Expect = 4.6e-202
Identity = 346/370 (93.51%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCETF KSLIDEVQKWGCMKQTGVSLRYMMEFG KPT KNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGLKPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLS KPAVLKVRDWYLDSFRDLR+FPEIK+ DDE+EFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYLDSFRDLRAFPEIKNPDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVP MALGVQQLKKS+   +IG+QDL EIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPMMALGVQQLKKSIALDSIGYQDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPDCVGYIHTKMSP++VA+SASEDARAICLREYGSAPNIKIYGDP+FTFPYVPTHLQLM
Sbjct: 181 PPPDCVGYIHTKMSPVKVAESASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLQLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQERFMDSDKV PPV+IIVADGIEDVTIKVSDEGGGIPRSGLP+IFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVKIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTA+NPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. ExPASy Swiss-Prot
Match: Q9SBJ1 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PDK PE=1 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 8.5e-183
Identity = 311/371 (83.83%), Postives = 338/371 (91.11%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MA KK CE FPKSLI++V KWGCMKQTGVSLRYMMEFGSKPTE+NLLISAQFLHKELPIR
Sbjct: 1   MAVKKACEMFPKSLIEDVHKWGCMKQTGVSLRYMMEFGSKPTERNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           +ARRAIEL+ LPYGLS KPAVLKVRDWYL+SFRD+R+FPEIK S DE++FTQMIKA+KVR
Sbjct: 61  VARRAIELQTLPYGLSDKPAVLKVRDWYLESFRDMRAFPEIKDSGDEKDFTQMIKAVKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVP MALGV QLKK +   N     L EIHQFLDRFY+SRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPMMALGVNQLKKGMNSGN-----LDEIHQFLDRFYLSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PP   VGYIHTKMSPMEVA++ASEDAR+IC REYGSAP I IYGDP+FTFPYVPTHL LM
Sbjct: 181 PPLHTVGYIHTKMSPMEVARNASEDARSICFREYGSAPEINIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           ++ELVKNSLRAVQERF+DSD+V PP+RIIVADGIEDVTIKVSDEGGGI RSGLP+IFTYL
Sbjct: 241 MYELVKNSLRAVQERFVDSDRVAPPIRIIVADGIEDVTIKVSDEGGGIARSGLPRIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADL-VTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHL 360
           Y+TARNPL+E  DLG AD+ VTMAGYGYGLPISRLYARYFGGDLQ+ISMEGYGTDAYLHL
Sbjct: 301 YSTARNPLEEDVDLGIADVPVTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHL 360

Query: 361 SRLGDSQEPLP 371
           SRLGDSQEPLP
Sbjct: 361 SRLGDSQEPLP 366

BLAST of Moc05g07640 vs. ExPASy Swiss-Prot
Match: Q9P6P9 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pkp1 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.3e-70
Identity = 163/426 (38.26%), Postives = 237/426 (55.63%), Query Frame = 0

Query: 12  KSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIRIARRAIELENL 71
           K+L ++V       QTG+SL+ ++ FG  PT   L  +  FL  ELPIR+ARR  +L+NL
Sbjct: 6   KTLQEKVNLLAQYPQTGLSLKQLVYFGKNPTPGTLFRAGLFLRDELPIRLARRIQDLQNL 65

Query: 72  PYGLSAKPAVLKVRDWYLDSFRDL------------------RSFPEIKSS--DDE---- 131
              L +   +  V+  Y  S  ++                   + P+ +SS  D E    
Sbjct: 66  SPMLRSMKRISSVKAAYGRSMEEIIELKGVELPKCLPKHARYHNAPKWRSSLMDSEILHN 125

Query: 132 ---------------------------------------QEFTQMIKAIKVRHNNVVPTM 191
                                                    F  ++  I+ RH+NV   +
Sbjct: 126 PSLANTHLDSSKGRYFETDFSDQDNGVDCNWPESLLKFNSNFAYLLNTIRTRHDNVAVEI 185

Query: 192 ALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPNPPPDCVGY 251
           AL +Q+ ++        +Q    I  FLDRFYMSRIGIRML+GQ++ L +  P  + VG 
Sbjct: 186 ALDIQEYRRKT------NQIDNSIQIFLDRFYMSRIGIRMLLGQYIALVSEPPRENYVGV 245

Query: 252 IHTKMSPMEVAQSASEDARAICLREYG--SAPNIKIYGDPNFTFPYVPTHLQLMVFELVK 311
           I T+ +  ++ + A+E+A+ IC   YG   AP I+I  DP+    YV +HL   VFE++K
Sbjct: 246 ISTRANIYQIIEGAAENAKYICRLAYGLFEAPEIQIICDPSLEMMYVESHLNHAVFEILK 305

Query: 312 NSLRA-VQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYLYTTAR 370
           NSLRA V+   +DSD  +PP+++IVA G ED+TIK+SDEGGGI R  +P +++Y++TTA 
Sbjct: 306 NSLRATVEFHGVDSD-FFPPIKVIVAKGQEDITIKISDEGGGISRRNIPLVWSYMFTTAS 365

BLAST of Moc05g07640 vs. ExPASy Swiss-Prot
Match: Q63065 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial OS=Rattus norvegicus OX=10116 GN=Pdk1 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.2e-66
Identity = 151/357 (42.30%), Postives = 213/357 (59.66%), Query Frame = 0

Query: 29  VSLRYMMEFGS-KPTEKNLLISAQFLHKELPIRIARRAIELENLPYGLSAKPAVLKVRDW 88
           +S++  ++FGS    EK    S  FL +ELP+R+A    E+  LP  L   P+V  V+ W
Sbjct: 57  LSMKQFLDFGSVNACEKT---SFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSW 116

Query: 89  YLDSFRDLRSFPEIKSSDDEQ---EFTQMIKAIKVRHNNVVPTMALGVQQLKKSLGPSNI 148
           Y+ S ++L  F + KS++D +   EFT  +  I+ RHN+V+PTMA GV + K+S G   +
Sbjct: 117 YIQSLQELLDFKD-KSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPV 176

Query: 149 GHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPNPPPD---CVGYIHTKMSPMEVAQS 208
             Q+   +  FLDRFYMSRI IRML+ QH  L      P     +G I+     +EV + 
Sbjct: 177 TSQN---VQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKD 236

Query: 209 ASEDARAICLREYGSAP-------NIKIYGDPNFTFPYVPTHLQLMVFELVKNSLRAVQE 268
             E+AR +C   Y ++P       N K  G P     YVP+HL  MVFEL KN++RA  E
Sbjct: 237 GYENARRLCDLYYVNSPELELEELNAKSPGQP-IQVVYVPSHLYHMVFELFKNAMRATME 296

Query: 269 RFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYLYTTARNPLDEHPDL 328
              D   VYPP+++ V  G ED+T+K+SD GGG+P   + ++F Y+Y+TA  P  E    
Sbjct: 297 HHADKG-VYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVE---- 356

Query: 329 GTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLSRLG-DSQEPLP 371
            T+  V +AG+GYGLPISRLYA+YF GDL++ S+EGYGTDA +++  L  +S E LP
Sbjct: 357 -TSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESIERLP 399

BLAST of Moc05g07640 vs. ExPASy Swiss-Prot
Match: Q8BFP9 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial OS=Mus musculus OX=10090 GN=Pdk1 PE=1 SV=2)

HSP 1 Score: 253.8 bits (647), Expect = 2.9e-66
Identity = 149/356 (41.85%), Postives = 214/356 (60.11%), Query Frame = 0

Query: 29  VSLRYMMEFGS-KPTEKNLLISAQFLHKELPIRIARRAIELENLPYGLSAKPAVLKVRDW 88
           +S++  ++FGS    EK    S  FL +ELP+R+A    E+  LP  L   P+V  V+ W
Sbjct: 57  LSMKQFLDFGSVNACEKT---SFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSW 116

Query: 89  YLDSFRDLRSFPEIKSSDDEQ---EFTQMIKAIKVRHNNVVPTMALGVQQLKKSLGPSNI 148
           Y+ S ++L  F + KS++D +   EFT  +  I+ RHN+V+PTMA GV + K+S G   +
Sbjct: 117 YIQSLQELLDFKD-KSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPV 176

Query: 149 GHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPNPPPD---CVGYIHTKMSPMEVAQS 208
             Q+   +  FLDRFYMSRI IRML+ QH  L      P     +G I+     +EV + 
Sbjct: 177 TSQN---VQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKD 236

Query: 209 ASEDARAICLREYGSAPNIKI----YGDPNFTFP--YVPTHLQLMVFELVKNSLRAVQER 268
             E+AR +C   Y ++P +++       P  T    YVP+HL  MVFEL KN++RA  E 
Sbjct: 237 GYENARRLCDLYYVNSPELELEELNAKSPGQTIQVVYVPSHLYHMVFELFKNAMRATMEH 296

Query: 269 FMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYLYTTARNPLDEHPDLG 328
             D   VYPP+++ V  G ED+T+K+SD GGG+P   + ++F Y+Y+TA  P  E     
Sbjct: 297 HADKG-VYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVE----- 356

Query: 329 TADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLSRLG-DSQEPLP 371
           T+  V +AG+GYGLPISRLYA+YF GDL++ S+EGYGTDA +++  L  +S E LP
Sbjct: 357 TSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESVERLP 399

BLAST of Moc05g07640 vs. ExPASy Swiss-Prot
Match: P91622 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Pdk PE=2 SV=2)

HSP 1 Score: 249.2 bits (635), Expect = 7.2e-65
Identity = 146/352 (41.48%), Postives = 207/352 (58.81%), Query Frame = 0

Query: 29  VSLRYMMEFGSKPTEKNLLISAQFLHKELPIRIARRAIELENLPYGLSAKPAVLKVRDWY 88
           +S++  M+FG    EK   I   FL KELP+R+A    E+  LP  L    +V +V  WY
Sbjct: 33  LSIKQFMDFGQNACEKKSYI---FLRKELPVRLANIMKEIALLPDNLLHTRSVSEVSSWY 92

Query: 89  LDSFRDLRSFPEIKSSDDE-QEFTQMIKAIKVRHNNVVPTMALGVQQLKKSLGPSNIGHQ 148
           + SF D+  + + + + D  Q+F   +  I+ RHN+VV TMA GV ++K++ G   +   
Sbjct: 93  VKSFEDVLVYEKAEPTHDNLQKFVADLDLIRNRHNDVVQTMAQGVIEMKENEG-GQVDAP 152

Query: 149 DLREIHQFLDRFYMSRIGIRMLIGQHVELH--NPNPPPDCVGYIHTKMSPMEVAQSASED 208
               I  FLDR YMSRI IRMLI QH  L   NP+     +G +       +V + A E+
Sbjct: 153 TESSIQYFLDRLYMSRISIRMLINQHTLLFGGNPHAGGRHIGCLDPACDLSDVVRDAYEN 212

Query: 209 ARAICLREYGSAPNIKIY---GDPNFTFP----YVPTHLQLMVFELVKNSLRAVQERF-M 268
           AR +C + Y ++P ++I     +P    P    YVP+HL  M+FEL KNS+RAV E    
Sbjct: 213 ARFLCDQYYLTSPALEIQQHSSEPGDNLPIRTVYVPSHLYYMLFELFKNSMRAVVEHHGH 272

Query: 269 DSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYLYTTARNPLDEHPDLGTA 328
           D++   PP+++ +  G ED+ +K+SD+GGGIPRS   ++F Y+Y+TA  P     DL T 
Sbjct: 273 DNNDTLPPLKVAICKGKEDICVKISDQGGGIPRSQTDQLFKYMYSTAPQP--SKSDLHT- 332

Query: 329 DLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLSRLGDSQEPL 370
             V +AGYGYGLPISRLYARYF GD+ ++S EG+GTDA ++L  L D    L
Sbjct: 333 --VPLAGYGYGLPISRLYARYFHGDIVLLSCEGFGTDAIIYLKALSDEANEL 375

BLAST of Moc05g07640 vs. ExPASy TrEMBL
Match: A0A6J1CAI4 (Protein-serine/threonine kinase OS=Momordica charantia OX=3673 GN=LOC111009461 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 6.1e-216
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM
Sbjct: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. ExPASy TrEMBL
Match: A0A6J1IPR6 (Protein-serine/threonine kinase OS=Cucurbita maxima OX=3661 GN=LOC111479408 PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 5.9e-203
Identity = 347/370 (93.78%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCETF KSLIDEVQKWGCMKQTGVSLRYMMEFG KPT KNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGLKPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLS KPAVLKVRDWYLDSFRDLR+FPEIK+ DDE+EFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYLDSFRDLRAFPEIKNPDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVP MALGVQQLKKS+   NIG+QDL EIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPMMALGVQQLKKSIALDNIGYQDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPDCVGYIHTKMSP++VA+SASEDARAICLREYGSAPNIKIYGDP+FTFPYVPTHLQLM
Sbjct: 181 PPPDCVGYIHTKMSPVKVAESASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLQLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQERFMDSDKV PPV+IIVADGIEDVTIKVSDEGGGIPRSGLP+IFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVKIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTA+NPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. ExPASy TrEMBL
Match: A0A0A0L2H2 (Protein-serine/threonine kinase OS=Cucumis sativus OX=3659 GN=Csa_4G304770 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 7.7e-203
Identity = 347/370 (93.78%), Postives = 361/370 (97.57%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCE+F KSLID+VQKWGCMKQTGVSLRYMMEFGSKPT KNLLISAQFL+KELPIR
Sbjct: 1   MAAKKLCESFSKSLIDDVQKWGCMKQTGVSLRYMMEFGSKPTAKNLLISAQFLYKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLS KPAVLKVRDWYLDSFRDLRSFPEIK+SDDE+EFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYLDSFRDLRSFPEIKNSDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVPTMALGVQQLKK L  +N+G+Q+L EIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVQQLKKGLRLNNVGYQNLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPDCVGYIHTKMSP++VA+SASEDARAICLREYGSAPNIKIYGDP+FTFPYVPTHL LM
Sbjct: 181 PPPDCVGYIHTKMSPVKVAESASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQERFMDSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTA+NPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. ExPASy TrEMBL
Match: A0A6J1GY59 (Protein-serine/threonine kinase OS=Cucurbita moschata OX=3662 GN=LOC111457914 PE=3 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 5.0e-202
Identity = 345/370 (93.24%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCETF KSLIDEVQKWGCMKQTGVSLRYMMEFG KPT +NLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGLKPTPRNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLS KPAVLKVRDWYLDSFRDLR+FPEIK+ DDE+EFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYLDSFRDLRAFPEIKNPDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVP MALGVQQLKKS+   +IG+QDL EIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPMMALGVQQLKKSIALDSIGYQDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPDCVGYIHTKMSP++VA+SASEDARAICLREYGSAPNIKIYGDP+FTFPYVPTHLQLM
Sbjct: 181 PPPDCVGYIHTKMSPVKVAESASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLQLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQERFMDSDKV PPV+IIVADGIEDVTIKVSDEGGGIPRSGLP+IFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVKIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTA+NPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. ExPASy TrEMBL
Match: A0A0A0L984 (Protein-serine/threonine kinase OS=Cucumis sativus OX=3659 GN=Csa_3G171100 PE=3 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.2e-200
Identity = 344/370 (92.97%), Postives = 356/370 (96.22%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MAAKKLCETF K+LIDEVQKWGCMKQTGVSLRYMMEFGSKPT KNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKTLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           IARRAIELENLPYGLS KPAVLKVRDWY+DSFRDLRSFPEIKSSDDE+EFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSMKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVPTMALGV+QLKK LG +N+G  DL EIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVKQLKKGLGLNNVGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PPPDCVGYIHTKMSP+ VAQSASEDARAICLREYGSAP+IKIYGDP+FTFPYVPTHL LM
Sbjct: 181 PPPDCVGYIHTKMSPVNVAQSASEDARAICLREYGSAPDIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           VFELVKNSLRAVQE FMDSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLP+IFTYL
Sbjct: 241 VFELVKNSLRAVQESFMDSDKVPPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTA+ PL+EHPDLGT DLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKEPLEEHPDLGTTDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of Moc05g07640 vs. TAIR 10
Match: AT3G06483.1 (pyruvate dehydrogenase kinase )

HSP 1 Score: 641.0 bits (1652), Expect = 6.0e-184
Identity = 311/371 (83.83%), Postives = 338/371 (91.11%), Query Frame = 0

Query: 1   MAAKKLCETFPKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTEKNLLISAQFLHKELPIR 60
           MA KK CE FPKSLI++V KWGCMKQTGVSLRYMMEFGSKPTE+NLLISAQFLHKELPIR
Sbjct: 1   MAVKKACEMFPKSLIEDVHKWGCMKQTGVSLRYMMEFGSKPTERNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSAKPAVLKVRDWYLDSFRDLRSFPEIKSSDDEQEFTQMIKAIKVR 120
           +ARRAIEL+ LPYGLS KPAVLKVRDWYL+SFRD+R+FPEIK S DE++FTQMIKA+KVR
Sbjct: 61  VARRAIELQTLPYGLSDKPAVLKVRDWYLESFRDMRAFPEIKDSGDEKDFTQMIKAVKVR 120

Query: 121 HNNVVPTMALGVQQLKKSLGPSNIGHQDLREIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVVP MALGV QLKK +   N     L EIHQFLDRFY+SRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPMMALGVNQLKKGMNSGN-----LDEIHQFLDRFYLSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDCVGYIHTKMSPMEVAQSASEDARAICLREYGSAPNIKIYGDPNFTFPYVPTHLQLM 240
           PP   VGYIHTKMSPMEVA++ASEDAR+IC REYGSAP I IYGDP+FTFPYVPTHL LM
Sbjct: 181 PPLHTVGYIHTKMSPMEVARNASEDARSICFREYGSAPEINIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQERFMDSDKVYPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300
           ++ELVKNSLRAVQERF+DSD+V PP+RIIVADGIEDVTIKVSDEGGGI RSGLP+IFTYL
Sbjct: 241 MYELVKNSLRAVQERFVDSDRVAPPIRIIVADGIEDVTIKVSDEGGGIARSGLPRIFTYL 300

Query: 301 YTTARNPLDEHPDLGTADL-VTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHL 360
           Y+TARNPL+E  DLG AD+ VTMAGYGYGLPISRLYARYFGGDLQ+ISMEGYGTDAYLHL
Sbjct: 301 YSTARNPLEEDVDLGIADVPVTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHL 360

Query: 361 SRLGDSQEPLP 371
           SRLGDSQEPLP
Sbjct: 361 SRLGDSQEPLP 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138237.11.3e-215100.00pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Momordica ch... [more]
XP_004147906.33.2e-20394.05pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucumis sati... [more]
XP_022979822.11.2e-20293.78pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucurbita ma... [more]
XP_038894097.13.5e-20294.32pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial-like isoform ... [more]
XP_023528572.14.6e-20293.51pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucurbita pe... [more]
Match NameE-valueIdentityDescription
Q9SBJ18.5e-18383.83[Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Arabidop... [more]
Q9P6P93.3e-7038.26[Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Schizosa... [more]
Q630652.2e-6642.30[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial O... [more]
Q8BFP92.9e-6641.85[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial O... [more]
P916227.2e-6541.48[Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Drosophi... [more]
Match NameE-valueIdentityDescription
A0A6J1CAI46.1e-216100.00Protein-serine/threonine kinase OS=Momordica charantia OX=3673 GN=LOC111009461 P... [more]
A0A6J1IPR65.9e-20393.78Protein-serine/threonine kinase OS=Cucurbita maxima OX=3661 GN=LOC111479408 PE=3... [more]
A0A0A0L2H27.7e-20393.78Protein-serine/threonine kinase OS=Cucumis sativus OX=3659 GN=Csa_4G304770 PE=3 ... [more]
A0A6J1GY595.0e-20293.24Protein-serine/threonine kinase OS=Cucurbita moschata OX=3662 GN=LOC111457914 PE... [more]
A0A0A0L9841.2e-20092.97Protein-serine/threonine kinase OS=Cucumis sativus OX=3659 GN=Csa_3G171100 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT3G06483.16.0e-18483.83pyruvate dehydrogenase kinase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004358Signal transduction histidine kinase-related protein, C-terminalPRINTSPR00344BCTRLSENSORcoord: 347..360
score: 28.11
coord: 277..291
score: 40.34
coord: 323..341
score: 35.59
IPR003594Histidine kinase/HSP90-like ATPaseSMARTSM00387HKATPase_4coord: 232..363
e-value: 1.0E-13
score: 61.6
IPR003594Histidine kinase/HSP90-like ATPasePFAMPF02518HATPase_ccoord: 234..360
e-value: 1.8E-14
score: 54.2
IPR036784Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamilyGENE3D1.20.140.20coord: 6..182
e-value: 4.0E-64
score: 217.7
IPR036784Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamilySUPERFAMILY69012alpha-ketoacid dehydrogenase kinase, N-terminal domaincoord: 16..180
IPR018955Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase, N-terminalPFAMPF10436BCDHK_Adom3coord: 28..190
e-value: 7.0E-57
score: 191.8
IPR036890Histidine kinase/HSP90-like ATPase superfamilyGENE3D3.30.565.10coord: 192..369
e-value: 1.0E-52
score: 180.0
IPR036890Histidine kinase/HSP90-like ATPase superfamilySUPERFAMILY55874ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinasecoord: 189..359
IPR039028PDK/BCKDK protein kinasePANTHERPTHR11947PYRUVATE DEHYDROGENASE KINASEcoord: 10..370
NoneNo IPR availablePANTHERPTHR11947:SF42BNAA05G30880D PROTEINcoord: 10..370
NoneNo IPR availableCDDcd16929HATPase_PDK-likecoord: 194..360
e-value: 1.78582E-77
score: 234.157
IPR005467Histidine kinase domainPROSITEPS50109HIS_KINcoord: 234..363
score: 18.234234

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g07640.1Moc05g07640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0010906 regulation of glucose metabolic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0005524 ATP binding
molecular_function GO:0009927 histidine phosphotransfer kinase activity
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0004740 pyruvate dehydrogenase (acetyl-transferring) kinase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0016772 transferase activity, transferring phosphorus-containing groups