Moc05g05220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g05220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	ADP-ribosylation factor-like protein 5
Location	chr5: 3631129 .. 3632575 (+)
RNA-Seq Expression	Moc05g05220
Synteny	Moc05g05220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGGCTTTCATATCAAGATTCTGGTTCATGTTATTTCCGGCGAAAGAATACAAGATCGTGGTCGTAGGGCTGGATAATGCCGGAAAGACGACGACTCTTTACAAATTGCACCTGGGAGAGGTTGTTACTACCCATCCTACTGTTGGAAGCAATGTTGAAGAACTTGTGTACAAGAATATTCGATTCGAGGTGCAGTATTTTGCCTTCTTCTTATTCTATTTTGTTTATGAATGCTCCTTGTTTGTTGCTCATCACATTCCATCGATTGGGGATTCTTTTCCGGCATCAATTTTATTCTAGCATTCTATTCGAGCATTGGTTTGTAAGGTTTTGCACTGTGATTCTTGAACTCTACGGCTTCGTTGGTGTATTGTTGCCCCTTGATTTTGTGAGTCGGTGTATTTGGTAGATGCAAAAGACTACCCGACTCAACCATAGAAACGAAAATGTGGTGCGCAAGAAATAGTAAAGCGTGTGTTGTCTGTAAACTTGAAACTTAGCGTGCAAAATACAAAATTAGGCGTGATAGTATAAAGCAACATATTCCCTTGTCATCTTCCTGGAGGCTAGTTCTGTAAAACCCAGGCTTTGATAAGATATGTTTCGTAAAGAGCTTTTATCGGAACCTTCCTGAAATCCCACACAATTTGCAGCTTTTTTAAGGGAAATTTGGGTGGATTAGGTTTATAATTGATTTGAGTAACTTAGCCGTCAAGAAGAAATGGATTCAAACCACGATGACCACGTACCTTGGATTTAATATCCCATTAGTTTTTTTGTCAACCTATCGTAGTAGGGTCAAGTGGTTAGTCGAGGTGTACGTAAGCTAGTCCGGAGACACGAATATTAAAAAAAATGATTTGAGTAAGTTCTCCACATAATTGGGCTGGTTTGTTTTCCAAACAATGGAACTGGAAAGCAGAGTTTTATCTGGAAAAGTATATACTGGCTTATTCAAAGACCAGATATATTATAAACTTTGCACAAAACCCGTCTCAATTTTTCATCTTGGAAGGCGAAACTGAACTTTTCCCTCTGATGCTGAGTAACTAAACCTGTCTTTTTTTGGCTATGTTTTCTATAGGTATGGGATCTTGGTGGGCAAGACAGGCTTAGGACATCATGGGCAACATATTATCGCGGTACCCATGCAGTAATTGTGGTGATAGACAGTACAGATAGAGCAAGAATCACCATTATGAAAGATGAGCTCTTCGGACTACTTGGCCACGAAGATCTTCAGCAATCTGTGGTGCTCGTTTTTGCAAACAAACAGGATCTCAAGGATGCCATGACCCCAGTTGAAATCACCGATGCCCTTTCACTTCACAGCATCAAGAATCACGACTGGCACATCCAAGCCTGCTCTGCCCTCACCGGAGACGGTCTATACGATGGTCTCGGGTGGATTGCTCAGCGAGTTACTGGTAAAGCACCCAGTTGA

mRNA sequence

ATGGGGGCTTTCATATCAAGATTCTGGTTCATGTTATTTCCGGCGAAAGAATACAAGATCGTGGTCGTAGGGCTGGATAATGCCGGAAAGACGACGACTCTTTACAAATTGCACCTGGGAGAGGTTGTTACTACCCATCCTACTGTTGGAAGCAATGTTGAAGAACTTGTGTACAAGAATATTCGATTCGAGGTATGGGATCTTGGTGGGCAAGACAGGCTTAGGACATCATGGGCAACATATTATCGCGGTACCCATGCAGTAATTGTGGTGATAGACAGTACAGATAGAGCAAGAATCACCATTATGAAAGATGAGCTCTTCGGACTACTTGGCCACGAAGATCTTCAGCAATCTGTGGTGCTCGTTTTTGCAAACAAACAGGATCTCAAGGATGCCATGACCCCAGTTGAAATCACCGATGCCCTTTCACTTCACAGCATCAAGAATCACGACTGGCACATCCAAGCCTGCTCTGCCCTCACCGGAGACGGTCTATACGATGGTCTCGGGTGGATTGCTCAGCGAGTTACTGGTAAAGCACCCAGTTGA

Coding sequence (CDS)

ATGGGGGCTTTCATATCAAGATTCTGGTTCATGTTATTTCCGGCGAAAGAATACAAGATCGTGGTCGTAGGGCTGGATAATGCCGGAAAGACGACGACTCTTTACAAATTGCACCTGGGAGAGGTTGTTACTACCCATCCTACTGTTGGAAGCAATGTTGAAGAACTTGTGTACAAGAATATTCGATTCGAGGTATGGGATCTTGGTGGGCAAGACAGGCTTAGGACATCATGGGCAACATATTATCGCGGTACCCATGCAGTAATTGTGGTGATAGACAGTACAGATAGAGCAAGAATCACCATTATGAAAGATGAGCTCTTCGGACTACTTGGCCACGAAGATCTTCAGCAATCTGTGGTGCTCGTTTTTGCAAACAAACAGGATCTCAAGGATGCCATGACCCCAGTTGAAATCACCGATGCCCTTTCACTTCACAGCATCAAGAATCACGACTGGCACATCCAAGCCTGCTCTGCCCTCACCGGAGACGGTCTATACGATGGTCTCGGGTGGATTGCTCAGCGAGTTACTGGTAAAGCACCCAGTTGA

Protein sequence

MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKNIRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSVVLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGKAPS

Homology

[Close]

BLAST of Moc05g05220 vs. NCBI nr
Match: XP_022138154.1 (ADP-ribosylation factor-like protein 5 [Momordica charantia] >XP_022138155.1 ADP-ribosylation factor-like protein 5 [Momordica charantia])

HSP 1 Score: 379.0 bits (972), Expect = 2.3e-101
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV
Sbjct: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK
Sbjct: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180

Query: 181 APS 184
           APS
Sbjct: 181 APS 183

[Close]

BLAST of Moc05g05220 vs. NCBI nr
Match: XP_008438599.1 (PREDICTED: ADP-ribosylation factor-like protein 5 [Cucumis melo])

HSP 1 Score: 370.2 bits (949), Expect = 1.1e-98
Identity = 178/183 (97.27%), Postives = 181/183 (98.91%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQ+RLRTSWATYYRGTHAVI VIDSTDRARITIMKDELF LLGHEDLQQ+V
Sbjct: 61  IRFEVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARITIMKDELFRLLGHEDLQQAV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQ+VTGK
Sbjct: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK 180

Query: 181 APS 184
           APS
Sbjct: 181 APS 183

[Close]

BLAST of Moc05g05220 vs. NCBI nr
Match: KAA0049318.1 (ADP-ribosylation factor-like protein 5 [Cucumis melo var. makuwa] >TYK17240.1 ADP-ribosylation factor-like protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 370.2 bits (949), Expect = 1.1e-98
Identity = 178/183 (97.27%), Postives = 181/183 (98.91%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 63  MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 122

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQ+RLRTSWATYYRGTHAVI VIDSTDRARITIMKDELF LLGHEDLQQ+V
Sbjct: 123 IRFEVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARITIMKDELFRLLGHEDLQQAV 182

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQ+VTGK
Sbjct: 183 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK 242

Query: 181 APS 184
           APS
Sbjct: 243 APS 245

[Close]

BLAST of Moc05g05220 vs. NCBI nr
Match: XP_022924719.1 (ADP-ribosylation factor-like protein 5 [Cucurbita moschata] >XP_022980057.1 ADP-ribosylation factor-like protein 5 [Cucurbita maxima] >XP_023527196.1 ADP-ribosylation factor-like protein 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 368.2 bits (944), Expect = 4.0e-98
Identity = 178/183 (97.27%), Postives = 180/183 (98.36%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAF+SRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFMSRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMK+ELF LLGHEDLQQSV
Sbjct: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKEELFRLLGHEDLQQSV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQ VTGK
Sbjct: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQHVTGK 180

Query: 181 APS 184
           A S
Sbjct: 181 ATS 183

[Close]

BLAST of Moc05g05220 vs. NCBI nr
Match: KAG5230669.1 (ADP-ribosylation factor family protein [Salix suchowensis])

HSP 1 Score: 368.2 bits (944), Expect = 4.0e-98
Identity = 176/183 (96.17%), Postives = 179/183 (97.81%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAF+SRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFVSRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDR RI+IMKDELFGLLGH+DLQ SV
Sbjct: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRDRISIMKDELFGLLGHDDLQHSV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTP EITDALSLHSIKNHDWHIQAC ALTGDGLYDGLGWIAQRVTGK
Sbjct: 121 VLVFANKQDLKDAMTPAEITDALSLHSIKNHDWHIQACCALTGDGLYDGLGWIAQRVTGK 180

Query: 181 APS 184
           APS
Sbjct: 181 APS 183

[Close]

BLAST of Moc05g05220 vs. ExPASy Swiss-Prot
Match: Q55AD9 (ADP-ribosylation factor-like protein 5 OS=Dictyostelium discoideum OX=44689 GN=arl5 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 7.0e-61
Identity = 110/180 (61.11%), Postives = 142/180 (78.89%), Query Frame = 0


Query: 1   MGAFISRF---WFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELV 60
           MG+ +S F   W   F   EYK+++VGL+ AGKTTTLYKL L EVV+T PTVGSN+EE V
Sbjct: 1   MGSSLSIFASIWNRFFNNAEYKVIIVGLNAAGKTTTLYKLLLDEVVSTTPTVGSNLEEFV 60

Query: 61  YKNIRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQ 120
           Y+NIR  +WDLGGQD LR++W  YY  T AVI+VIDSTDRAR+ ++K+ELF +L HE+L+
Sbjct: 61  YRNIRLLMWDLGGQDLLRSTWNQYYINTQAVILVIDSTDRARVNLIKEELFKMLAHENLK 120

Query: 121 QSVVLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRV 178
           +S++L++ANKQDLKDAM+P E++  LSLHSIK+HD+HIQAC ALTG GL  GL W+   +
Sbjct: 121 KSIILIYANKQDLKDAMSPTELSTLLSLHSIKDHDYHIQACCALTGQGLESGLDWLVSHI 180

[Close]

BLAST of Moc05g05220 vs. ExPASy Swiss-Prot
Match: Q9Y689 (ADP-ribosylation factor-like protein 5A OS=Homo sapiens OX=9606 GN=ARL5A PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.8e-56
Identity = 101/177 (57.06%), Postives = 134/177 (75.71%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MG   +R W  LF  +E+K+++VGLDNAGKTT LY+  + EVV T PT+GSNVEE+V  N
Sbjct: 1   MGILFTRIW-RLFNHQEHKVIIVGLDNAGKTTILYQFSMNEVVHTSPTIGSNVEEIVINN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
            RF +WD+GGQ+ LR+SW TYY  T  VIVV+DSTDR RI++ ++EL+ +L HEDL+++ 
Sbjct: 61  TRFLMWDIGGQESLRSSWNTYYTNTEFVIVVVDSTDRERISVTREELYKMLAHEDLRKAG 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRV 178
           +L+FANKQD+K+ MT  EI+  L L SIK+H WHIQAC ALTG+GL  GL W+  R+
Sbjct: 121 LLIFANKQDVKECMTVAEISQFLKLTSIKDHQWHIQACCALTGEGLCQGLEWMMSRL 176

[Close]

BLAST of Moc05g05220 vs. ExPASy Swiss-Prot
Match: P51646 (ADP-ribosylation factor-like protein 5A OS=Rattus norvegicus OX=10116 GN=Arl5a PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.8e-56
Identity = 101/177 (57.06%), Postives = 134/177 (75.71%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MG   +R W  LF  +E+K+++VGLDNAGKTT LY+  + EVV T PT+GSNVEE+V  N
Sbjct: 1   MGILFTRIW-RLFNHQEHKVIIVGLDNAGKTTILYQFSMNEVVHTSPTIGSNVEEIVVNN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
            RF +WD+GGQ+ LR+SW TYY  T  VIVV+DSTDR RI++ ++EL+ +L HEDL+++ 
Sbjct: 61  TRFLMWDIGGQESLRSSWNTYYTNTEFVIVVVDSTDRERISVTREELYKMLAHEDLRKAG 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRV 178
           +L+FANKQD+K+ MT  EI+  L L SIK+H WHIQAC ALTG+GL  GL W+  R+
Sbjct: 121 LLIFANKQDVKECMTVAEISQFLKLTSIKDHQWHIQACCALTGEGLCQGLEWMMSRL 176

[Close]

BLAST of Moc05g05220 vs. ExPASy Swiss-Prot
Match: Q80ZU0 (ADP-ribosylation factor-like protein 5A OS=Mus musculus OX=10090 GN=Arl5a PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 3.0e-56
Identity = 101/177 (57.06%), Postives = 133/177 (75.14%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MG   +R W  LF  +E+K+++VGLDNAGKTT LY+  + EVV T PT+GSNVEE+V  N
Sbjct: 1   MGILFTRIW-RLFNHQEHKVIIVGLDNAGKTTILYQFSMNEVVHTSPTIGSNVEEIVVNN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
            RF +WD+GGQ+ LR SW TYY  T  VIVV+DSTDR RI++ ++EL+ +L HEDL+++ 
Sbjct: 61  TRFLMWDIGGQESLRPSWNTYYTNTEFVIVVVDSTDRERISVTREELYKMLAHEDLRKAG 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRV 178
           +L+FANKQD+K+ MT  EI+  L L SIK+H WHIQAC ALTG+GL  GL W+  R+
Sbjct: 121 LLIFANKQDVKECMTVAEISQFLKLTSIKDHQWHIQACCALTGEGLCQGLEWMMSRL 176

[Close]

BLAST of Moc05g05220 vs. ExPASy Swiss-Prot
Match: Q2KJ96 (ADP-ribosylation factor-like protein 5A OS=Bos taurus OX=9913 GN=ARL5A PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 4.0e-56
Identity = 101/177 (57.06%), Postives = 133/177 (75.14%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MG   +R W  LF  +E+K+++VGLDNAGKTT LY+  + EVV T PT+GSNVEE+V  N
Sbjct: 1   MGILFTRIW-RLFNHQEHKVIIVGLDNAGKTTILYQFSMNEVVHTSPTIGSNVEEIVINN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
            RF +WD+GGQ+ LR+SW TYY  T  VIVV+DSTDR RI++ ++EL+ +L HEDL+++ 
Sbjct: 61  TRFLMWDIGGQESLRSSWNTYYTNTEFVIVVVDSTDRERISVTREELYKMLAHEDLRKAG 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRV 178
           +L+FANKQD+K  MT  EI+  L L SIK+H WHIQAC ALTG+GL  GL W+  R+
Sbjct: 121 LLIFANKQDVKGCMTVAEISQFLKLTSIKDHQWHIQACCALTGEGLCQGLEWMMSRL 176

[Close]

BLAST of Moc05g05220 vs. ExPASy TrEMBL
Match: A0A6J1C994 (ADP-ribosylation factor-like protein 5 OS=Momordica charantia OX=3673 GN=LOC111009394 PE=3 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.1e-101
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV
Sbjct: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK
Sbjct: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180

Query: 181 APS 184
           APS
Sbjct: 181 APS 183

[Close]

BLAST of Moc05g05220 vs. ExPASy TrEMBL
Match: A0A5A7U254 (ADP-ribosylation factor-like protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00910 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.1e-99
Identity = 178/183 (97.27%), Postives = 181/183 (98.91%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 63  MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 122

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQ+RLRTSWATYYRGTHAVI VIDSTDRARITIMKDELF LLGHEDLQQ+V
Sbjct: 123 IRFEVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARITIMKDELFRLLGHEDLQQAV 182

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQ+VTGK
Sbjct: 183 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK 242

Query: 181 APS 184
           APS
Sbjct: 243 APS 245

[Close]

BLAST of Moc05g05220 vs. ExPASy TrEMBL
Match: A0A1S3AWS5 (ADP-ribosylation factor-like protein 5 OS=Cucumis melo OX=3656 GN=LOC103483660 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.1e-99
Identity = 178/183 (97.27%), Postives = 181/183 (98.91%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQ+RLRTSWATYYRGTHAVI VIDSTDRARITIMKDELF LLGHEDLQQ+V
Sbjct: 61  IRFEVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARITIMKDELFRLLGHEDLQQAV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQ+VTGK
Sbjct: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK 180

Query: 181 APS 184
           APS
Sbjct: 181 APS 183

[Close]

BLAST of Moc05g05220 vs. ExPASy TrEMBL
Match: A0A6J1IQD8 (ADP-ribosylation factor-like protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111479573 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.9e-98
Identity = 178/183 (97.27%), Postives = 180/183 (98.36%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAF+SRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFMSRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMK+ELF LLGHEDLQQSV
Sbjct: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKEELFRLLGHEDLQQSV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQ VTGK
Sbjct: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQHVTGK 180

Query: 181 APS 184
           A S
Sbjct: 181 ATS 183

[Close]

BLAST of Moc05g05220 vs. ExPASy TrEMBL
Match: A0A6J1EA03 (ADP-ribosylation factor-like protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111432135 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.9e-98
Identity = 178/183 (97.27%), Postives = 180/183 (98.36%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAF+SRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFMSRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMK+ELF LLGHEDLQQSV
Sbjct: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKEELFRLLGHEDLQQSV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQ VTGK
Sbjct: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQHVTGK 180

Query: 181 APS 184
           A S
Sbjct: 181 ATS 183

[Close]

BLAST of Moc05g05220 vs. TAIR 10
Match: AT3G22950.1 (ADP-ribosylation factor C1 )

HSP 1 Score: 350.1 bits (897), Expect = 1.1e-96
Identity = 167/183 (91.26%), Postives = 175/183 (95.63%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAF+SRFWFM+FPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFMSRFWFMMFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARI+ MKDEL  LLGHEDLQ SV
Sbjct: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARISFMKDELARLLGHEDLQNSV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           +LVFANKQDLKDAMTP EITDAL+LHSIKNHDWHIQA  A+TG+GLYDGLGWIAQ+VTGK
Sbjct: 121 ILVFANKQDLKDAMTPAEITDALNLHSIKNHDWHIQASCAVTGEGLYDGLGWIAQKVTGK 180

Query: 181 APS 184
           A S
Sbjct: 181 ATS 183

[Close]

BLAST of Moc05g05220 vs. TAIR 10
Match: AT3G22950.2 (ADP-ribosylation factor C1 )

HSP 1 Score: 350.1 bits (897), Expect = 1.1e-96
Identity = 167/183 (91.26%), Postives = 175/183 (95.63%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MGAF+SRFWFM+FPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN
Sbjct: 1   MGAFMSRFWFMMFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARI+ MKDEL  LLGHEDLQ SV
Sbjct: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARISFMKDELARLLGHEDLQNSV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           +LVFANKQDLKDAMTP EITDAL+LHSIKNHDWHIQA  A+TG+GLYDGLGWIAQ+VTGK
Sbjct: 121 ILVFANKQDLKDAMTPAEITDALNLHSIKNHDWHIQASCAVTGEGLYDGLGWIAQKVTGK 180

Query: 181 APS 184
           A S
Sbjct: 181 ATS 183

[Close]

BLAST of Moc05g05220 vs. TAIR 10
Match: AT1G70490.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 206.1 bits (523), Expect = 2.5e-53
Identity = 94/181 (51.93%), Postives = 129/181 (71.27%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MG   ++ +  LF  KE +I++VGLD AGKTT LYKL LGE+VTT PT+G NVE + YKN
Sbjct: 1   MGLSFAKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           I F VWD+GGQD++R  W  Y++ T  +I V+DS DR R+   +DEL  +L  ++L+ +V
Sbjct: 61  ISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           +LVFANKQDL +AM   EITD L LHS++   W+IQ+  A +G+GLY+GL W++  + GK
Sbjct: 121 LLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIAGK 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]

BLAST of Moc05g05220 vs. TAIR 10
Match: AT1G23490.1 (ADP-ribosylation factor 1 )

HSP 1 Score: 206.1 bits (523), Expect = 2.5e-53
Identity = 94/181 (51.93%), Postives = 129/181 (71.27%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MG   ++ +  LF  KE +I++VGLD AGKTT LYKL LGE+VTT PT+G NVE + YKN
Sbjct: 1   MGLSFAKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           I F VWD+GGQD++R  W  Y++ T  +I V+DS DR R+   +DEL  +L  ++L+ +V
Sbjct: 61  ISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           +LVFANKQDL +AM   EITD L LHS++   W+IQ+  A +G+GLY+GL W++  + GK
Sbjct: 121 LLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIAGK 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]

BLAST of Moc05g05220 vs. TAIR 10
Match: AT1G70490.3 (Ras-related small GTP-binding family protein )

HSP 1 Score: 206.1 bits (523), Expect = 2.5e-53
Identity = 94/181 (51.93%), Postives = 129/181 (71.27%), Query Frame = 0


Query: 1   MGAFISRFWFMLFPAKEYKIVVVGLDNAGKTTTLYKLHLGEVVTTHPTVGSNVEELVYKN 60
           MG   ++ +  LF  KE +I++VGLD AGKTT LYKL LGE+VTT PT+G NVE + YKN
Sbjct: 1   MGLSFAKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKN 60

Query: 61  IRFEVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARITIMKDELFGLLGHEDLQQSV 120
           I F VWD+GGQD++R  W  Y++ T  +I V+DS DR R+   +DEL  +L  ++L+ +V
Sbjct: 61  ISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAV 120

Query: 121 VLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQRVTGK 180
           +LVFANKQDL +AM   EITD L LHS++   W+IQ+  A +G+GLY+GL W++  + GK
Sbjct: 121 LLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIAGK 180

Query: 181 A 182
           A
Sbjct: 181 A 181

The following BLAST results are available for this feature:

BLAST of Moc05g05220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138154.1	2.3e-101	100.00	ADP-ribosylation factor-like protein 5 [Momordica charantia] >XP_022138155.1 ADP...	[more]
XP_008438599.1	1.1e-98	97.27	PREDICTED: ADP-ribosylation factor-like protein 5 [Cucumis melo]	[more]
KAA0049318.1	1.1e-98	97.27	ADP-ribosylation factor-like protein 5 [Cucumis melo var. makuwa] >TYK17240.1 AD...	[more]
XP_022924719.1	4.0e-98	97.27	ADP-ribosylation factor-like protein 5 [Cucurbita moschata] >XP_022980057.1 ADP-...	[more]
KAG5230669.1	4.0e-98	96.17	ADP-ribosylation factor family protein [Salix suchowensis]	[more]

BLAST of Moc05g05220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q55AD9	7.0e-61	61.11	ADP-ribosylation factor-like protein 5 OS=Dictyostelium discoideum OX=44689 GN=a...	[more]
Q9Y689	1.8e-56	57.06	ADP-ribosylation factor-like protein 5A OS=Homo sapiens OX=9606 GN=ARL5A PE=1 SV...	[more]
P51646	1.8e-56	57.06	ADP-ribosylation factor-like protein 5A OS=Rattus norvegicus OX=10116 GN=Arl5a P...	[more]
Q80ZU0	3.0e-56	57.06	ADP-ribosylation factor-like protein 5A OS=Mus musculus OX=10090 GN=Arl5a PE=1 S...	[more]
Q2KJ96	4.0e-56	57.06	ADP-ribosylation factor-like protein 5A OS=Bos taurus OX=9913 GN=ARL5A PE=2 SV=1	[more]

BLAST of Moc05g05220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C994	1.1e-101	100.00	ADP-ribosylation factor-like protein 5 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5A7U254	5.1e-99	97.27	ADP-ribosylation factor-like protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3AWS5	5.1e-99	97.27	ADP-ribosylation factor-like protein 5 OS=Cucumis melo OX=3656 GN=LOC103483660 P...	[more]
A0A6J1IQD8	1.9e-98	97.27	ADP-ribosylation factor-like protein 5 OS=Cucurbita maxima OX=3661 GN=LOC1114795...	[more]
A0A6J1EA03	1.9e-98	97.27	ADP-ribosylation factor-like protein 5 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of Moc05g05220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22950.1	1.1e-96	91.26	ADP-ribosylation factor C1	[more]
AT3G22950.2	1.1e-96	91.26	ADP-ribosylation factor C1	[more]
AT1G70490.1	2.5e-53	51.93	Ras-related small GTP-binding family protein	[more]
AT1G23490.1	2.5e-53	51.93	ADP-ribosylation factor 1	[more]
AT1G70490.3	2.5e-53	51.93	Ras-related small GTP-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006689	Small GTPase superfamily, ARF/SAR type	PRINTS	PR00328	SAR1GTPBP	coord: 74..99 score: 31.45 coord: 19..42 score: 53.68 coord: 119..140 score: 27.27 coord: 47..71 score: 44.47
IPR006689	Small GTPase superfamily, ARF/SAR type	SMART	SM00178	sar_sub_1	coord: 1..177 e-value: 5.5E-19 score: 79.1
IPR006689	Small GTPase superfamily, ARF/SAR type	PFAM	PF00025	Arf	coord: 14..176 e-value: 1.7E-64 score: 216.5
None	No IPR available	SMART	SM00175	rab_sub_5	coord: 18..180 e-value: 1.3E-4 score: -52.1
None	No IPR available	SMART	SM00177	arf_sub_2	coord: 1..181 e-value: 3.4E-77 score: 272.4
None	No IPR available	PANTHER	PTHR11711:SF369	ADP-RIBOSYLATION FACTOR C1	coord: 1..183
None	No IPR available	PANTHER	PTHR11711	ADP RIBOSYLATION FACTOR-RELATED	coord: 1..183
None	No IPR available	PROSITE	PS51417	ARF	coord: 11..177 score: 22.715645
None	No IPR available	PROSITE	PS51419	RAB	coord: 1..183 score: 9.055016
None	No IPR available	CDD	cd04153	Arl5_Arl8	coord: 3..176 e-value: 1.47793E-119 score: 333.547
IPR005225	Small GTP-binding protein domain	TIGRFAM	TIGR00231	TIGR00231	coord: 16..145 e-value: 5.1E-22 score: 76.4
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 6..182 e-value: 6.0E-67 score: 226.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 15..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g05220.1	Moc05g05220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:1903292	protein localization to Golgi membrane
biological_process	GO:0016192	vesicle-mediated transport
cellular_component	GO:0005802	trans-Golgi network
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding