Moc05g05090 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g05090
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionDNA-directed RNA polymerase V subunit 7-like
Locationchr5: 3497276 .. 3497857 (+)
RNA-Seq ExpressionMoc05g05090
SyntenyMoc05g05090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTGTGAAGTGGAACTAGCAAGAGATGTGGAAGTCATGGCTGAAAATGAAAAGAGAGATGGTTTCAACAACTTTCACAGGTACATTATAACATGTCTATTAGAAAACCTATTGAAGGAGAAGGCCAGCAGAGATCATGGCTATTTTCTTGCTGTTACTAACTTGAAAAGCATAGGCAAGGGAGAGGTTAAGAATGAGTCTCAATCTCAATGCATTTCATTCCCGGTGACGTTTAGTTGCCGAACCTTTTTGCCTTTCGAGGGGGAGATCTTGCACGGAGTTGTTCGTCACATATTTAGGCGTGGATTACTCTTAAGATGTGGACCTATCAAGTATGTGTTTCTCTCAGCTCGTAAAATGCCAACCTACCGTTACGTCGGTGGCAAAAATCCACTTTTCTCGAGTGACGAATGTGCGACAATAGAAAACGACGTGGTTGTACGTTTCAGTGTGCTTGGTGTGAGATGGATAGAGAAGAGAGGATGCATCAAAAAGGAGTTTGTGATGCTTGCTAGCTTAGAAGGTAACAACTCACTCGGTCCGATTTCTTCGTCCGAATCGGATGAGTTTGACTTGTAA

mRNA sequence

ATGTTTTGTGAAGTGGAACTAGCAAGAGATGTGGAAGTCATGGCTGAAAATGAAAAGAGAGATGGTTTCAACAACTTTCACAGGTACATTATAACATGTCTATTAGAAAACCTATTGAAGGAGAAGGCCAGCAGAGATCATGGCTATTTTCTTGCTGTTACTAACTTGAAAAGCATAGGCAAGGGAGAGGTTAAGAATGAGTCTCAATCTCAATGCATTTCATTCCCGGTGACGTTTAGTTGCCGAACCTTTTTGCCTTTCGAGGGGGAGATCTTGCACGGAGTTGTTCGTCACATATTTAGGCGTGGATTACTCTTAAGATGTGGACCTATCAAGTATGTGTTTCTCTCAGCTCGTAAAATGCCAACCTACCGTTACGTCGGTGGCAAAAATCCACTTTTCTCGAGTGACGAATGTGCGACAATAGAAAACGACGTGGTTGTACGTTTCAGTGTGCTTGGTGTGAGATGGATAGAGAAGAGAGGATGCATCAAAAAGGAGTTTGTGATGCTTGCTAGCTTAGAAGGTAACAACTCACTCGGTCCGATTTCTTCGTCCGAATCGGATGAGTTTGACTTGTAA

Coding sequence (CDS)

ATGTTTTGTGAAGTGGAACTAGCAAGAGATGTGGAAGTCATGGCTGAAAATGAAAAGAGAGATGGTTTCAACAACTTTCACAGGTACATTATAACATGTCTATTAGAAAACCTATTGAAGGAGAAGGCCAGCAGAGATCATGGCTATTTTCTTGCTGTTACTAACTTGAAAAGCATAGGCAAGGGAGAGGTTAAGAATGAGTCTCAATCTCAATGCATTTCATTCCCGGTGACGTTTAGTTGCCGAACCTTTTTGCCTTTCGAGGGGGAGATCTTGCACGGAGTTGTTCGTCACATATTTAGGCGTGGATTACTCTTAAGATGTGGACCTATCAAGTATGTGTTTCTCTCAGCTCGTAAAATGCCAACCTACCGTTACGTCGGTGGCAAAAATCCACTTTTCTCGAGTGACGAATGTGCGACAATAGAAAACGACGTGGTTGTACGTTTCAGTGTGCTTGGTGTGAGATGGATAGAGAAGAGAGGATGCATCAAAAAGGAGTTTGTGATGCTTGCTAGCTTAGAAGGTAACAACTCACTCGGTCCGATTTCTTCGTCCGAATCGGATGAGTTTGACTTGTAA

Protein sequence

MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIGKGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARKMPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSLGPISSSESDEFDL
Homology
BLAST of Moc05g05090 vs. NCBI nr
Match: XP_022147266.1 (DNA-directed RNA polymerase V subunit 7-like [Momordica charantia])

HSP 1 Score: 399.8 bits (1026), Expect = 1.3e-107
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG
Sbjct: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK
Sbjct: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL
Sbjct: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180

Query: 181 GPISSSESDEFDL 194
           GPISSSESDEFDL
Sbjct: 181 GPISSSESDEFDL 193

BLAST of Moc05g05090 vs. NCBI nr
Match: XP_022924760.1 (DNA-directed RNA polymerase V subunit 7-like [Cucurbita moschata] >XP_023528651.1 DNA-directed RNA polymerase V subunit 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 349.4 bits (895), Expect = 2.0e-92
Identity = 168/193 (87.05%), Postives = 179/193 (92.75%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MFCEVEL RDVEV  E EKRDG  N  RYIITCLLENLLKEKAS+DHGYFLAVT+LKSIG
Sbjct: 1   MFCEVELVRDVEVTVEKEKRDGV-NLQRYIITCLLENLLKEKASKDHGYFLAVTSLKSIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           KGEVKNES+SQC+SFP+TFSCRTFLP EGEILHGVVRHIFRRGLLL+CGPIKYVFLSARK
Sbjct: 61  KGEVKNESESQCVSFPITFSCRTFLPLEGEILHGVVRHIFRRGLLLKCGPIKYVFLSARK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTY YVGG+NP+FSS + ATI NDVVVRFSVLGVRW+EKRGCIKKEFVMLASLEGN SL
Sbjct: 121 MPTYHYVGGENPVFSSTQSATIGNDVVVRFSVLGVRWLEKRGCIKKEFVMLASLEGNTSL 180

Query: 181 GPISSSESDEFDL 194
           GPISSS+SDEFDL
Sbjct: 181 GPISSSDSDEFDL 192

BLAST of Moc05g05090 vs. NCBI nr
Match: KAG7018763.1 (DNA-directed RNA polymerase subunit 7-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 347.4 bits (890), Expect = 7.7e-92
Identity = 167/193 (86.53%), Postives = 178/193 (92.23%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MFCEVEL RDVEV  E EKRDG  N  RYIITCLLENLLKEKAS+DHGYFLAVT+LKSIG
Sbjct: 76  MFCEVELVRDVEVTVEKEKRDGV-NLQRYIITCLLENLLKEKASKDHGYFLAVTSLKSIG 135

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           KGEVKNES+SQC+SFP+TF CRTFLP EGEILHGVVRHIFRRGLLL+CGPIKYVFLSARK
Sbjct: 136 KGEVKNESESQCVSFPITFRCRTFLPLEGEILHGVVRHIFRRGLLLKCGPIKYVFLSARK 195

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTY YVGG+NP+FSS + ATI NDVVVRFSVLGVRW+EKRGCIKKEFVMLASLEGN SL
Sbjct: 196 MPTYHYVGGENPVFSSTQSATIGNDVVVRFSVLGVRWLEKRGCIKKEFVMLASLEGNTSL 255

Query: 181 GPISSSESDEFDL 194
           GPISSS+SDEFDL
Sbjct: 256 GPISSSDSDEFDL 267

BLAST of Moc05g05090 vs. NCBI nr
Match: KAG6582352.1 (DNA-directed RNA polymerase subunit 7-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 344.7 bits (883), Expect = 5.0e-91
Identity = 167/193 (86.53%), Postives = 178/193 (92.23%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF EVEL RDVEV  E EKRDG  N  RYIITCLLENLLKEKAS+DHGYFLAVT+LKSIG
Sbjct: 32  MFREVELVRDVEVTVEKEKRDGV-NLQRYIITCLLENLLKEKASKDHGYFLAVTSLKSIG 91

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           KGEVKNES+SQC+SFP+TFSCRTFLP EGEILHGVVRHIFRRGLLL+CGPIKYVFLSARK
Sbjct: 92  KGEVKNESESQCVSFPITFSCRTFLPLEGEILHGVVRHIFRRGLLLKCGPIKYVFLSARK 151

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTY YVGG+NP+FSS + ATI NDVVVRFSVLGVRW+EKRGCIKKEFVMLASLEGN SL
Sbjct: 152 MPTYHYVGGENPVFSSTQSATIGNDVVVRFSVLGVRWLEKRGCIKKEFVMLASLEGNTSL 211

Query: 181 GPISSSESDEFDL 194
           GPISSS+SDEFDL
Sbjct: 212 GPISSSDSDEFDL 223

BLAST of Moc05g05090 vs. NCBI nr
Match: XP_022980115.1 (DNA-directed RNA polymerase V subunit 7-like isoform X1 [Cucurbita maxima] >XP_022980117.1 DNA-directed RNA polymerase V subunit 7-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-89
Identity = 164/193 (84.97%), Postives = 176/193 (91.19%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MFCEVEL R+VEV  E EKRDG  N  RYIITCLLENLLKEKAS+DHGYFLAVT+LKSIG
Sbjct: 1   MFCEVELVRNVEVTVEKEKRDGV-NLQRYIITCLLENLLKEKASKDHGYFLAVTSLKSIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           K EVKNES+SQC+SFP+TFSCRTFLP EGEILHGVVRHIFRRGLLL+CGPIKYVFLSARK
Sbjct: 61  KEEVKNESESQCVSFPITFSCRTFLPLEGEILHGVVRHIFRRGLLLKCGPIKYVFLSARK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTY YVGG+NP+FSS + ATI NDVVVRFSVLGVRW+EKRGCI KEFVMLASLEGN SL
Sbjct: 121 MPTYHYVGGENPVFSSTQSATIGNDVVVRFSVLGVRWLEKRGCINKEFVMLASLEGNTSL 180

Query: 181 GPISSSESDEFDL 194
           GPIS S+SDEFDL
Sbjct: 181 GPISLSDSDEFDL 192

BLAST of Moc05g05090 vs. ExPASy Swiss-Prot
Match: Q6NML5 (DNA-directed RNA polymerase subunit 7-like protein OS=Arabidopsis thaliana OX=3702 GN=NRPB7L PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-39
Identity = 87/200 (43.50%), Postives = 125/200 (62.50%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF EVE+ARDV + A   K     + H+ I+  LL++L+ EKA R+HG++L +T LKSIG
Sbjct: 1   MFSEVEMARDVAICA---KHLNGQSPHQPILCRLLQDLIHEKACREHGFYLGITALKSIG 60

Query: 61  KG-----EVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVF 120
                  + +N  Q++ ++FPV+F+CRTFLP  G+IL G V+ +   G  +R GP++Y +
Sbjct: 61  NNKNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAY 120

Query: 121 LSARKMPTYRYVGG-----KNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVM 180
           LS  KMP Y YV       + P F  D+ + I   VVVRF VL VR+ E+    + ++ +
Sbjct: 121 LSLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYV 180

Query: 181 LASLEGNNSLGPISSSESDE 191
           LA+LEGN S GPIS + SDE
Sbjct: 181 LATLEGNGSFGPISLTGSDE 197

BLAST of Moc05g05090 vs. ExPASy Swiss-Prot
Match: A6QRA1 (DNA-directed RNA polymerase V subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPE7 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 6.3e-36
Identity = 77/184 (41.85%), Postives = 121/184 (65.76%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF +V+L  +V + AEN    G     R I+  LLE    +KA+++ GY++AVT L  IG
Sbjct: 1   MFLKVQLPWNVMIPAENMDAKGL-MLKRAILVELLEAFASKKATKELGYYVAVTTLDKIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           +G+++  +    + FPV FS  TF  F+GEI+HGVV  + + G+ +RCGPI+ V+LS  K
Sbjct: 61  EGKIREHTGE--VLFPVMFSGMTFKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MP Y+Y+ G+NP+F +++ + I+ +  VR  V+G++W+E    +++EF  LASLEG + L
Sbjct: 121 MPDYKYIPGENPIFMNEKTSRIQVETTVRVVVIGIKWME----VEREFQALASLEG-DYL 176

Query: 181 GPIS 185
           GP+S
Sbjct: 181 GPLS 176

BLAST of Moc05g05090 vs. ExPASy Swiss-Prot
Match: Q8LE42 (DNA-directed RNA polymerase IV subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPD7 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-29
Identity = 73/182 (40.11%), Postives = 110/182 (60.44%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF +V+L  DV + AE+   D      R I+  LLE   KEKA++D GY +  T L++IG
Sbjct: 1   MFIKVKLPWDVTIPAED--MDTGLMLQRAIVIRLLEAFSKEKATKDLGYLITPTILENIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           +G++K ++    I FPV F+   F  F+GEI+HGVV  + + G+ L+ GP + ++LS  K
Sbjct: 61  EGKIKEQTGE--IQFPVVFNGICFKMFKGEIVHGVVHKVHKTGVFLKSGPYEIIYLSHMK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MP Y ++ G+NP F +   + I+    VRF VL   W E     +K+F+ LAS++G+N L
Sbjct: 121 MPGYEFIPGENPFFMNQYMSRIQIGARVRFVVLDTEWRE----AEKDFMALASIDGDN-L 173

Query: 181 GP 183
           GP
Sbjct: 181 GP 173

BLAST of Moc05g05090 vs. ExPASy Swiss-Prot
Match: P46279 (DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.7e-13
Identity = 44/136 (32.35%), Postives = 74/136 (54.41%), Query Frame = 0

Query: 26  FHRYIITCLLENLLKE---KASRDHGYFLAVTNLKSIGKGEVKNESQSQCISFPVTFSCR 85
           F R +   L+  L+K+     S  HG+ +AVT +++IGKG +++   +  ++FPV + C 
Sbjct: 18  FGRNLRDNLVSKLMKDVEGTCSGRHGFVVAVTGIENIGKGLIRD--GTGFVTFPVKYQCV 77

Query: 86  TFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARKMP-TYRYVGGKNPLF-SSDECA 145
            F PF+GEIL  VV  + + G     GP++ +F+S   +P    +  G  P + +SD   
Sbjct: 78  VFRPFKGEILEAVVTMVNKMGFFAEAGPVQ-IFVSNHLIPDDMEFQSGDMPNYTTSDGSV 137

Query: 146 TIENDVVVRFSVLGVR 157
            I+ D  VR  ++G R
Sbjct: 138 KIQKDSEVRLKIIGTR 150

BLAST of Moc05g05090 vs. ExPASy TrEMBL
Match: A0A6J1D1U6 (DNA-directed RNA polymerase V subunit 7-like OS=Momordica charantia OX=3673 GN=LOC111016261 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 6.4e-108
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG
Sbjct: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK
Sbjct: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL
Sbjct: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180

Query: 181 GPISSSESDEFDL 194
           GPISSSESDEFDL
Sbjct: 181 GPISSSESDEFDL 193

BLAST of Moc05g05090 vs. ExPASy TrEMBL
Match: A0A6J1EFY9 (DNA-directed RNA polymerase V subunit 7-like OS=Cucurbita moschata OX=3662 GN=LOC111432163 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 9.9e-93
Identity = 168/193 (87.05%), Postives = 179/193 (92.75%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MFCEVEL RDVEV  E EKRDG  N  RYIITCLLENLLKEKAS+DHGYFLAVT+LKSIG
Sbjct: 1   MFCEVELVRDVEVTVEKEKRDGV-NLQRYIITCLLENLLKEKASKDHGYFLAVTSLKSIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           KGEVKNES+SQC+SFP+TFSCRTFLP EGEILHGVVRHIFRRGLLL+CGPIKYVFLSARK
Sbjct: 61  KGEVKNESESQCVSFPITFSCRTFLPLEGEILHGVVRHIFRRGLLLKCGPIKYVFLSARK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTY YVGG+NP+FSS + ATI NDVVVRFSVLGVRW+EKRGCIKKEFVMLASLEGN SL
Sbjct: 121 MPTYHYVGGENPVFSSTQSATIGNDVVVRFSVLGVRWLEKRGCIKKEFVMLASLEGNTSL 180

Query: 181 GPISSSESDEFDL 194
           GPISSS+SDEFDL
Sbjct: 181 GPISSSDSDEFDL 192

BLAST of Moc05g05090 vs. ExPASy TrEMBL
Match: A0A6J1IQI7 (DNA-directed RNA polymerase V subunit 7-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479602 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.0e-90
Identity = 164/193 (84.97%), Postives = 176/193 (91.19%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MFCEVEL R+VEV  E EKRDG  N  RYIITCLLENLLKEKAS+DHGYFLAVT+LKSIG
Sbjct: 1   MFCEVELVRNVEVTVEKEKRDGV-NLQRYIITCLLENLLKEKASKDHGYFLAVTSLKSIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           K EVKNES+SQC+SFP+TFSCRTFLP EGEILHGVVRHIFRRGLLL+CGPIKYVFLSARK
Sbjct: 61  KEEVKNESESQCVSFPITFSCRTFLPLEGEILHGVVRHIFRRGLLLKCGPIKYVFLSARK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTY YVGG+NP+FSS + ATI NDVVVRFSVLGVRW+EKRGCI KEFVMLASLEGN SL
Sbjct: 121 MPTYHYVGGENPVFSSTQSATIGNDVVVRFSVLGVRWLEKRGCINKEFVMLASLEGNTSL 180

Query: 181 GPISSSESDEFDL 194
           GPIS S+SDEFDL
Sbjct: 181 GPISLSDSDEFDL 192

BLAST of Moc05g05090 vs. ExPASy TrEMBL
Match: A0A1S3AWE2 (DNA-directed RNA polymerase V subunit 7-like OS=Cucumis melo OX=3656 GN=LOC103483648 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.1e-87
Identity = 162/193 (83.94%), Postives = 176/193 (91.19%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF EVEL RDVE+  E EKRD  +NF RYIITCLLENLLKEKA++DHGYFL+VT+LKSIG
Sbjct: 1   MFYEVELVRDVEITVEKEKRDA-HNFQRYIITCLLENLLKEKANKDHGYFLSVTSLKSIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           KG VKNE  SQC+SFP+TF CRTFLPFEGEILHGVVRHIF+RGLLL+CGPIKY FLSARK
Sbjct: 61  KGVVKNE--SQCVSFPITFICRTFLPFEGEILHGVVRHIFQRGLLLKCGPIKYAFLSARK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTY+YVGG+NP+FSS E ATI NDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL
Sbjct: 121 MPTYQYVGGENPVFSSKEFATIGNDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180

Query: 181 GPISSSESDEFDL 194
           GPIS S+SDEFDL
Sbjct: 181 GPISLSDSDEFDL 190

BLAST of Moc05g05090 vs. ExPASy TrEMBL
Match: A0A5A7U0C3 (DNA-directed RNA polymerase V subunit 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001070 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.1e-87
Identity = 162/193 (83.94%), Postives = 176/193 (91.19%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF EVEL RDVE+  E EKRD  +NF RYIITCLLENLLKEKA++DHGYFL+VT+LKSIG
Sbjct: 1   MFYEVELVRDVEITVEKEKRDA-HNFQRYIITCLLENLLKEKANKDHGYFLSVTSLKSIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           KG VKNE  SQC+SFP+TF CRTFLPFEGEILHGVVRHIF+RGLLL+CGPIKY FLSARK
Sbjct: 61  KGVVKNE--SQCVSFPITFICRTFLPFEGEILHGVVRHIFQRGLLLKCGPIKYAFLSARK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MPTY+YVGG+NP+FSS E ATI NDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL
Sbjct: 121 MPTYQYVGGENPVFSSKEFATIGNDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180

Query: 181 GPISSSESDEFDL 194
           GPIS S+SDEFDL
Sbjct: 181 GPISLSDSDEFDL 190

BLAST of Moc05g05090 vs. TAIR 10
Match: AT4G14520.1 (DNA-directed RNA polymerase II-related )

HSP 1 Score: 163.7 bits (413), Expect = 1.5e-40
Identity = 87/200 (43.50%), Postives = 125/200 (62.50%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF EVE+ARDV + A   K     + H+ I+  LL++L+ EKA R+HG++L +T LKSIG
Sbjct: 1   MFSEVEMARDVAICA---KHLNGQSPHQPILCRLLQDLIHEKACREHGFYLGITALKSIG 60

Query: 61  KG-----EVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVF 120
                  + +N  Q++ ++FPV+F+CRTFLP  G+IL G V+ +   G  +R GP++Y +
Sbjct: 61  NNKNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAY 120

Query: 121 LSARKMPTYRYVGG-----KNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVM 180
           LS  KMP Y YV       + P F  D+ + I   VVVRF VL VR+ E+    + ++ +
Sbjct: 121 LSLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYV 180

Query: 181 LASLEGNNSLGPISSSESDE 191
           LA+LEGN S GPIS + SDE
Sbjct: 181 LATLEGNGSFGPISLTGSDE 197

BLAST of Moc05g05090 vs. TAIR 10
Match: AT4G14520.2 (DNA-directed RNA polymerase II-related )

HSP 1 Score: 163.7 bits (413), Expect = 1.5e-40
Identity = 87/200 (43.50%), Postives = 125/200 (62.50%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF EVE+ARDV + A   K     + H+ I+  LL++L+ EKA R+HG++L +T LKSIG
Sbjct: 1   MFSEVEMARDVAICA---KHLNGQSPHQPILCRLLQDLIHEKACREHGFYLGITALKSIG 60

Query: 61  KG-----EVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVF 120
                  + +N  Q++ ++FPV+F+CRTFLP  G+IL G V+ +   G  +R GP++Y +
Sbjct: 61  NNKNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAY 120

Query: 121 LSARKMPTYRYVGG-----KNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVM 180
           LS  KMP Y YV       + P F  D+ + I   VVVRF VL VR+ E+    + ++ +
Sbjct: 121 LSLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYV 180

Query: 181 LASLEGNNSLGPISSSESDE 191
           LA+LEGN S GPIS + SDE
Sbjct: 181 LATLEGNGSFGPISLTGSDE 197

BLAST of Moc05g05090 vs. TAIR 10
Match: AT4G14520.3 (DNA-directed RNA polymerase II-related )

HSP 1 Score: 163.7 bits (413), Expect = 1.5e-40
Identity = 87/200 (43.50%), Postives = 125/200 (62.50%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF EVE+ARDV + A   K     + H+ I+  LL++L+ EKA R+HG++L +T LKSIG
Sbjct: 1   MFSEVEMARDVAICA---KHLNGQSPHQPILCRLLQDLIHEKACREHGFYLGITALKSIG 60

Query: 61  KG-----EVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVF 120
                  + +N  Q++ ++FPV+F+CRTFLP  G+IL G V+ +   G  +R GP++Y +
Sbjct: 61  NNKNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAY 120

Query: 121 LSARKMPTYRYVGG-----KNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVM 180
           LS  KMP Y YV       + P F  D+ + I   VVVRF VL VR+ E+    + ++ +
Sbjct: 121 LSLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYV 180

Query: 181 LASLEGNNSLGPISSSESDE 191
           LA+LEGN S GPIS + SDE
Sbjct: 181 LATLEGNGSFGPISLTGSDE 197

BLAST of Moc05g05090 vs. TAIR 10
Match: AT4G14660.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 152.1 bits (383), Expect = 4.4e-37
Identity = 77/184 (41.85%), Postives = 121/184 (65.76%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF +V+L  +V + AEN    G     R I+  LLE    +KA+++ GY++AVT L  IG
Sbjct: 1   MFLKVQLPWNVMIPAENMDAKGL-MLKRAILVELLEAFASKKATKELGYYVAVTTLDKIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           +G+++  +    + FPV FS  TF  F+GEI+HGVV  + + G+ +RCGPI+ V+LS  K
Sbjct: 61  EGKIREHTGE--VLFPVMFSGMTFKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MP Y+Y+ G+NP+F +++ + I+ +  VR  V+G++W+E    +++EF  LASLEG + L
Sbjct: 121 MPDYKYIPGENPIFMNEKTSRIQVETTVRVVVIGIKWME----VEREFQALASLEG-DYL 176

Query: 181 GPIS 185
           GP+S
Sbjct: 181 GPLS 176

BLAST of Moc05g05090 vs. TAIR 10
Match: AT3G22900.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 131.3 bits (329), Expect = 8.1e-31
Identity = 73/182 (40.11%), Postives = 110/182 (60.44%), Query Frame = 0

Query: 1   MFCEVELARDVEVMAENEKRDGFNNFHRYIITCLLENLLKEKASRDHGYFLAVTNLKSIG 60
           MF +V+L  DV + AE+   D      R I+  LLE   KEKA++D GY +  T L++IG
Sbjct: 1   MFIKVKLPWDVTIPAED--MDTGLMLQRAIVIRLLEAFSKEKATKDLGYLITPTILENIG 60

Query: 61  KGEVKNESQSQCISFPVTFSCRTFLPFEGEILHGVVRHIFRRGLLLRCGPIKYVFLSARK 120
           +G++K ++    I FPV F+   F  F+GEI+HGVV  + + G+ L+ GP + ++LS  K
Sbjct: 61  EGKIKEQTGE--IQFPVVFNGICFKMFKGEIVHGVVHKVHKTGVFLKSGPYEIIYLSHMK 120

Query: 121 MPTYRYVGGKNPLFSSDECATIENDVVVRFSVLGVRWIEKRGCIKKEFVMLASLEGNNSL 180
           MP Y ++ G+NP F +   + I+    VRF VL   W E     +K+F+ LAS++G+N L
Sbjct: 121 MPGYEFIPGENPFFMNQYMSRIQIGARVRFVVLDTEWRE----AEKDFMALASIDGDN-L 173

Query: 181 GP 183
           GP
Sbjct: 181 GP 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147266.11.3e-107100.00DNA-directed RNA polymerase V subunit 7-like [Momordica charantia][more]
XP_022924760.12.0e-9287.05DNA-directed RNA polymerase V subunit 7-like [Cucurbita moschata] >XP_023528651.... [more]
KAG7018763.17.7e-9286.53DNA-directed RNA polymerase subunit 7-like protein, partial [Cucurbita argyrospe... [more]
KAG6582352.15.0e-9186.53DNA-directed RNA polymerase subunit 7-like protein, partial [Cucurbita argyrospe... [more]
XP_022980115.11.2e-8984.97DNA-directed RNA polymerase V subunit 7-like isoform X1 [Cucurbita maxima] >XP_0... [more]
Match NameE-valueIdentityDescription
Q6NML52.1e-3943.50DNA-directed RNA polymerase subunit 7-like protein OS=Arabidopsis thaliana OX=37... [more]
A6QRA16.3e-3641.85DNA-directed RNA polymerase V subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPE7... [more]
Q8LE421.1e-2940.11DNA-directed RNA polymerase IV subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPD... [more]
P462799.7e-1332.35DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D1U66.4e-108100.00DNA-directed RNA polymerase V subunit 7-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1EFY99.9e-9387.05DNA-directed RNA polymerase V subunit 7-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IQI76.0e-9084.97DNA-directed RNA polymerase V subunit 7-like isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A1S3AWE22.1e-8783.94DNA-directed RNA polymerase V subunit 7-like OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A5A7U0C32.1e-8783.94DNA-directed RNA polymerase V subunit 7-like OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
AT4G14520.11.5e-4043.50DNA-directed RNA polymerase II-related [more]
AT4G14520.21.5e-4043.50DNA-directed RNA polymerase II-related [more]
AT4G14520.31.5e-4043.50DNA-directed RNA polymerase II-related [more]
AT4G14660.14.4e-3741.85RNA polymerase Rpb7-like, N-terminal domain [more]
AT3G22900.18.1e-3140.11RNA polymerase Rpb7-like, N-terminal domain [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.40.50.140coord: 88..182
e-value: 8.7E-14
score: 53.2
NoneNo IPR availablePANTHERPTHR12709:SF6DNA-DIRECTED RNA POLYMERASE SUBUNIT 7-LIKE PROTEINcoord: 1..193
IPR036898RNA polymerase Rpb7-like, N-terminal domain superfamilyGENE3D3.30.1490.120coord: 1..87
e-value: 3.6E-12
score: 48.4
IPR036898RNA polymerase Rpb7-like, N-terminal domain superfamilySUPERFAMILY88798N-terminal, heterodimerisation domain of RBP7 (RpoE)coord: 24..84
IPR045113RNA polymerase Rpb7-likePANTHERPTHR12709DNA-DIRECTED RNA POLYMERASE II, IIIcoord: 1..193
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 86..158

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g05090.1Moc05g05090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006352 DNA-templated transcription, initiation
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0003727 single-stranded RNA binding