Moc05g00540 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g00540
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Inhibitor against trypsin
Location	chr5: 415615 .. 419304 (+)
RNA-Seq Expression	Moc05g00540
Synteny	Moc05g00540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGCGATGTCAAGGAAAGAGCTCATGGCCGCGGCTGGTGGGATCGACCAGCGCGGCGGCGAAGGCGGTCATCGAGAGGGAGAATCCTCGCGTCAGAGCCGTCATTATAAAGGTCGGAGCCCGCCACCCTGGACTTCCGCTGCGATCGGGTTCGGGTTTGGGTCACTGAGAGGGGAATCGTTGCAAGGCCACCCACAATTGGCTAACCGCCGATCCGACCCGACGAGTCCAAAATATATAAAGCAGAGATAATATATGCAATAAATATAATATTGGCAATTAGCACCGTACTACATAAAACTTCTGCTTCTGCTTATTGGTGTACATCACCCCAATTACTAAATAAAATAATACTGCTCAATAATTATATCATTTCCCCTCGACTTTATTATTATTATTTTTTCTTTCTAAAAATGCCGGAACCATTACAGTTTACTAGTTTTATTCTTTGAGTTCTTATTCAATTAATAAATCACATCAAACTTAGCTTCAAAAATATATATATATATATATAATCAAACTCATAATCAATTCAACTCAAATTTAAATCTAATTCACTTAATTAAGAAGTATATTTGCATCAAAATATTCCGAGTATATTTATTTGTTCTAAAGTTAAAAAATGATTTGATCCATAAATTATTCAAATGGTCTCTCAAAATTACTATTTTATTAACTAAATGACACTAATTGAGATTTTATTTGATGGGTTACTGTTGTTTTTGTTTGATGTTCCTATATAGTCAGGAGCAGATTCAGATTTACTATAGATTTCCAGCCTCGCTAAATTAGTTTCTTTTTTTTTTAGGGGAATATATGTATTATTAGTTAATAATTTACTTTGAAGTAATCAAACTAACAAAGTTGCACTTTGGCGGCGCTCGGCCCTCTAATAGCTTGACTTTGGGTCTCCAATTCATAATTCTCATACTGTTTTTTCTAAAGTACATTTTTTTTTTCTTAACTTTAAATATGATAGTTAGTAAAAGTTTGAGCTAATATAAAACAGTCGTTTGTAAATTATGTACTTGTAAATTCTTTTTACTGAATTCTAAATAAAGTCTCTATATTGATATTTATTTATATTTTGTTTATGTAACAATAATTTTCTATTTGTTTGGCTACTATTAAAGGTCAAACTAATAAAGGAAAAATATCCCGTAAACAAAATCACATCATCATATATAACAAAATATATCTAGCATAAGTTAATCATGTCTGATAAAATATATCTAACTAATAAGTTAATTATGTCACTAGCAGAATAATAATTTTAATGACTAAGCATAGATTTATACTATTTATTATTAATTTTTTTTACAAATATATTTTTAAGAAAAGGAATGTAAACAGGGAAGAAAGAAATTTTTAAAATAAATTAATTAATAACTATAAAATTTGCCATTCATTAGAGAATTAGTATTATTCTTTGGACAAATTTTATTTTAATTAAAACATTATTTTTTGTTATTTTTCTAAAGGCTAAAATAAATTAGCTCAGATTTTCAACGTATTTTAATTATAATAAATTGGTTGACTAATTTATGTTCATGCTTTGAGTTCTTTCACTATTTAATACACAAAATAAAGAGCTTATCTCATCGTGTAACATATTTCATCGTAAAGTTACTATTAAAAAAATGACAATTTATTATAATTTTAAGATTTCTCTATTTTTACATTCTTTTTTTTGTCATCAAATATTTAATGGTGGTGAGAATATAAGATAAATCATTACAAGTAAGAGGTAATTATTTTCTTAAAATAAATAATCAAATTACATTTTTGTCCCTAAATTTAACAACATTTTTGTTATATATCATTTTTCCTTTTAGTACATTTTTTTGTCATATATTATATATATTGGCTATTTTGTCAAAATAGTTTAATACAACACTAGATATATATAACAATAAAATAATAATGGTCCGAAATTGAAGGTCTTGCCAAAAAAATCAATGATATGTGTATGACAACGCTCACCGTCGCCGCTTAGCTTTAAAGATAAAATGCAACCCTGCAAAATTTTATCTCAAAAAGAGAAACCTTGTTATAAAAGAAAAAAATAAGCTACAAAATTTAATTTCAAATTAAAAATATGACAGTTGTGTACTAAAAAGTGTGTGTGTATATATATATACACGACAGGTGTTTTTTTTTAGTTAAAAGTGTGACAAGTAGTGTAGATCTAAAATGAGGTGTGAACTTTGGGTATATGTTAAGTATAATCTTTCATTAAGATTGAATGTTAACCGATTTAAGTGTAGTTAAGTTATATATCCTCGGCCAAAACGAGCATAGTTCAATGGTAATTGGCATATATACCTCCAACTATGTGATCATGAGTTCGATCTCCCACCCACACATGTTGTACTAAAAAAAAGTTATATGTCCTCGACTAAAATGTTAAAAATTTACATCCTTACATTACCTGTTGGACTTAAAAAATGTTAAAAATGTTGAAATCTTCGTTCAAACATTATAACATTCTACTCTCTAAAAAAATTGTAAAATAGTTGAAAAGTATACACTTATCAATTGGAGTATTAAAAGATCTAATGACCTGAAAATTCCAAATACAACCGCTTAAATTACAAATTTAGTCATACACTAGTATCTAATAGGTGTCTAAATAAATAAATAAATAATATATATATATATATATATATATATATATATATATATATATATTTGTCTAATAAGCATTCCGAACCAACTAGACATTTTTTCAAACTCATGTATTTATTAGACACGAAATTAAAAACTCGATGTGCAACTAAACCAAAAATTAAATTTTAAATATATATAAAATATTTAAAAATATTGAATTAATTATAGAACTATTATATTAGATACATTTTTTAAATTTAAGGACTATTAAACACGATTCTAAAAATTCATAACCTAATATCGTAATTTATTGCACATATATTTTACTCTCTAGCTAATAACATTTAGAAAATAATCCCCCGAGTTAAAAGTCAAATATTTTTCTGTTGAGCTGGACGATTAAATATATATAGTTATTCGAATGGAACAAAATTATACTCAAACAATTAGATTAAGTACTATAATTAGAAAGAGGCAACACCCATCTATTGCCACCATTAAAATAAAATTCTGACCAGAGAGAAAAATATAGAAGGAAATATACTTTTGTATTTTCCAAAGAATAAAAAGCAGTTAAATTGTTTTATTTCAAAGAAAAAAATAAGCAGTTAAATTTCTAGTAATTATCAGGACAGGCCACAGGGTAAGTATTTTTCATATAAATACCCCATCTCCTAGGAAAAGAAAAACATATTAATTGAGAGACAGTCATAAAGAAGTGAGAGAAAAGATGTCTCGATGTCAAGGTATAAGTTAATTATAAACTTGATTCTGTGTGTATTATATTTGTAATTTGTGTGTTTTTTTATTTATTTTTGAAAGGTGTAATTTGTGTGCGTTTAGTGTTAATTAATTAGTTAATGTTGTTATTAATTTATGATCAGGAAAGAGCTCATGGCCGCAGCTGGTGGGATCGACCGGCGCGGCGGCGAAGGCGGTCATCGAGAGGGAGAACCCTCGCGTGAGAGCCGTCATTATAAAGGTCGGATCGGGCGCCACCAAAGACTTCCGCTGCGACCGGGTTCGGGTTTGGGTCACTGAGAGGGGAATCGTCGCAAGGCCACCCACAATTGGCTAG

mRNA sequence

ATGTCGCGATGTCAAGGAAAGAGCTCATGGCCGCGGCTGGTGGGATCGACCAGCGCGGCGGCGAAGGCGGTCATCGAGAGGGAGAATCCTCGCGTCAGAGCCGTCATTATAAAGGTCGGAGCCCGCCACCCTGGACTTCCGCTGCGATCGGGTTCGGGAAAGAGCTCATGGCCGCAGCTGGTGGGATCGACCGGCGCGGCGGCGAAGGCGGTCATCGAGAGGGAGAACCCTCGCGTGAGAGCCGTCATTATAAAGGTCGGATCGGGCGCCACCAAAGACTTCCGCTGCGACCGGGTTCGGGTTTGGGTCACTGAGAGGGGAATCGTCGCAAGGCCACCCACAATTGGCTAG

Coding sequence (CDS)

ATGTCGCGATGTCAAGGAAAGAGCTCATGGCCGCGGCTGGTGGGATCGACCAGCGCGGCGGCGAAGGCGGTCATCGAGAGGGAGAATCCTCGCGTCAGAGCCGTCATTATAAAGGTCGGAGCCCGCCACCCTGGACTTCCGCTGCGATCGGGTTCGGGAAAGAGCTCATGGCCGCAGCTGGTGGGATCGACCGGCGCGGCGGCGAAGGCGGTCATCGAGAGGGAGAACCCTCGCGTGAGAGCCGTCATTATAAAGGTCGGATCGGGCGCCACCAAAGACTTCCGCTGCGACCGGGTTCGGGTTTGGGTCACTGAGAGGGGAATCGTCGCAAGGCCACCCACAATTGGCTAG

Protein sequence

MSRCQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGARHPGLPLRSGSGKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARPPTIG

Homology

[Close]

BLAST of Moc05g00540 vs. NCBI nr
Match: 1VBW_A (Crystal Structure of Bitter Gourd Trypsin Inhibitor [Momordica charantia] >prf||2111250B trypsin inhibitor BGIT [Momordica charantia])

HSP 1 Score: 131.3 bits (329), Expect = 5.2e-27
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP
Sbjct: 5   GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 64

Query: 113 PTIG 117
           PTIG
Sbjct: 65  PTIG 68

[Close]

BLAST of Moc05g00540 vs. NCBI nr
Match: BAB32588.1 (inhibitor against trypsin, partial [Momordica charantia])

HSP 1 Score: 131.3 bits (329), Expect = 5.2e-27
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP
Sbjct: 3   GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 62

Query: 113 PTIG 117
           PTIG
Sbjct: 63  PTIG 66

[Close]

BLAST of Moc05g00540 vs. NCBI nr
Match: P24076.1 (RecName: Full=Glu S.griseus protease inhibitor; AltName: Full=BGIA [Momordica charantia] >AAB19651.1 BGIA=acidic amino acid-specific endopeptidase inhibitor [Momordica charantia L.=bitter gourd, Peptide, 68 aa] [Momordica charantia])

HSP 1 Score: 119.0 bits (297), Expect = 2.7e-23
Identity = 57/64 (89.06%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GK SWPQLVGSTGAAAKAVIERENPRVRAVI++VGS  T DFRCDRVRVWVTERGIVARP
Sbjct: 5   GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 113 PTIG 117
           P IG
Sbjct: 65  PAIG 68

[Close]

BLAST of Moc05g00540 vs. NCBI nr
Match: KVF69647.1 (Proteinase inhibitor I13, potato inhibitor I [Cynara cardunculus var. scolymus])

HSP 1 Score: 111.3 bits (277), Expect = 5.6e-21
Identity = 60/116 (51.72%), Postives = 69/116 (59.48%), Query Frame = 0


Query: 1   MSRCQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGARHPGLPLRSGSGKSSWPQL 60
           M+ C GKSSWP LVG T  AA  +IERENP V A+++               GK SWP++
Sbjct: 1   MTDCAGKSSWPELVGRTGEAAXVIIERENPLVDAIVLL-------------DGKRSWPEV 60

Query: 61  VGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARPPTIG 117
           VG  G  A   IERENPRV A +I  G+    DFRCDRVRV V  RGIV R P IG
Sbjct: 61  VGRRGEDAVXTIERENPRVDAFVILDGTSVIGDFRCDRVRVRVNSRGIVVRTPEIG 103

[Close]

BLAST of Moc05g00540 vs. NCBI nr
Match: PQP96738.1 (Proteinase inhibitor [Prunus yedoensis var. nudiflora])

HSP 1 Score: 106.7 bits (265), Expect = 1.4e-19
Identity = 56/127 (44.09%), Postives = 73/127 (57.48%), Query Frame = 0


Query: 6   GKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA----------------RHPGLPLR 65
           GK SWP +VG +   A A IEREN  VRA++I  G+                R+  +   
Sbjct: 8   GKKSWPEVVGQSGEDAAAKIERENHNVRAIVILEGSATTLDRRCDRVWVWVNRNGTVTKA 67

Query: 66  SGSGKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIV 117
              GK SWP++VG +G  A A +EREN  VRA+++  GS ATKD RCDRVRVW+   G+V
Sbjct: 68  PKVGKQSWPEVVGHSGEDAAAKVERENHNVRAIVMLEGSPATKDLRCDRVRVWINRNGVV 127

[Close]

BLAST of Moc05g00540 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 3.5e-26
Identity = 57/64 (89.06%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GK SWPQLVGSTGAAAKAVIERENPRVRAVI++VGS  T DFRCDRVRVWVTERGIVARP
Sbjct: 5   GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 113 PTIG 117
           P IG
Sbjct: 65  PAIG 68

[Close]

BLAST of Moc05g00540 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-18
Identity = 41/64 (64.06%), Postives = 52/64 (81.25%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GKSSWP LVG  G+ AKA+IER+NP V+AVI++ G+  TKDFRC+RVR+WV +RG+V  P
Sbjct: 5   GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 113 PTIG 117
           P IG
Sbjct: 65  PRIG 68

[Close]

BLAST of Moc05g00540 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 7.6e-13
Identity = 36/67 (53.73%), Postives = 43/67 (64.18%), Query Frame = 0


Query: 49  RSGSGKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGI 108
           R   GK  WP+LVG  G  A A+IERENP VR+++    SG TKDFRCDRV V V   G+
Sbjct: 2   RECPGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGV 61

Query: 109 VARPPTI 116
           V R P +
Sbjct: 62  VVRTPRV 68

[Close]

BLAST of Moc05g00540 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 7.6e-13
Identity = 34/63 (53.97%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GK++WP+LVG +G  A A +EREN  V A+++K GS  TKDFRCDRV V V + G+V   
Sbjct: 6   GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSV 65

Query: 113 PTI 116
           P I
Sbjct: 66  PHI 68

[Close]

BLAST of Moc05g00540 vs. ExPASy Swiss-Prot
Match: P09407 (Trypsin inhibitor MCI-3 OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 6.5e-12
Identity = 46/90 (51.11%), Postives = 52/90 (57.78%), Query Frame = 0


Query: 27  RENPRVRAVIIKVGARHPGLPLRSGSGKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKV 86
           RE+P VRA++I VG         SG+ K      VGSTGAAAKA++ERENPRVRA     
Sbjct: 6   REDPSVRAIVISVG---------SGATKDK----VGSTGAAAKALVERENPRVRA----- 62

Query: 87  GSGATKDFRCDRVRVWVTERGIVARPPTIG 117
                          WVTERGIVARPPTIG
Sbjct: 66  ---------------WVTERGIVARPPTIG 62

[Close]

BLAST of Moc05g00540 vs. ExPASy TrEMBL
Match: Q9AYN0 (Inhibitor against trypsin (Fragment) OS=Momordica charantia OX=3673 GN=bgit PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.5e-27
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP
Sbjct: 3   GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 62

Query: 113 PTIG 117
           PTIG
Sbjct: 63  PTIG 66

[Close]

BLAST of Moc05g00540 vs. ExPASy TrEMBL
Match: Q7M1Q1 (Trypsin inhibitor BGIT OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.5e-27
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP
Sbjct: 5   GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 64

Query: 113 PTIG 117
           PTIG
Sbjct: 65  PTIG 68

[Close]

BLAST of Moc05g00540 vs. ExPASy TrEMBL
Match: A0A103L5L1 (Proteinase inhibitor I13, potato inhibitor I OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_026716 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.7e-21
Identity = 60/116 (51.72%), Postives = 69/116 (59.48%), Query Frame = 0


Query: 1   MSRCQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGARHPGLPLRSGSGKSSWPQL 60
           M+ C GKSSWP LVG T  AA  +IERENP V A+++               GK SWP++
Sbjct: 1   MTDCAGKSSWPELVGRTGEAAXVIIERENPLVDAIVLL-------------DGKRSWPEV 60

Query: 61  VGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARPPTIG 117
           VG  G  A   IERENPRV A +I  G+    DFRCDRVRV V  RGIV R P IG
Sbjct: 61  VGRRGEDAVXTIERENPRVDAFVILDGTSVIGDFRCDRVRVRVNSRGIVVRTPEIG 103

[Close]

BLAST of Moc05g00540 vs. ExPASy TrEMBL
Match: A0A314XVX5 (Proteinase inhibitor OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_08087 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.7e-20
Identity = 56/127 (44.09%), Postives = 73/127 (57.48%), Query Frame = 0


Query: 6   GKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA----------------RHPGLPLR 65
           GK SWP +VG +   A A IEREN  VRA++I  G+                R+  +   
Sbjct: 8   GKKSWPEVVGQSGEDAAAKIERENHNVRAIVILEGSATTLDRRCDRVWVWVNRNGTVTKA 67

Query: 66  SGSGKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIV 117
              GK SWP++VG +G  A A +EREN  VRA+++  GS ATKD RCDRVRVW+   G+V
Sbjct: 68  PKVGKQSWPEVVGHSGEDAAAKVERENHNVRAIVMLEGSPATKDLRCDRVRVWINRNGVV 127

[Close]

BLAST of Moc05g00540 vs. ExPASy TrEMBL
Match: A0A6D2KVQ0 (Uncharacterized protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS39306 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.9e-19
Identity = 61/122 (50.00%), Postives = 73/122 (59.84%), Query Frame = 0


Query: 7   KSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGARHPGLPLRSG------------SGK 66
           K++WP LVG     A +VIEREN RV AV++ VG+     PL +             + K
Sbjct: 3   KNAWPELVGKNGDFAASVIERENNRVDAVVVLVGS-----PLTADFKCNRVRVIVDRNAK 62

Query: 67  SSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARPPT 117
           ++WP+LV   G  A AVIEREN RV AV+I VGS AT DFRCDRVRVWV   GIV   P 
Sbjct: 63  NAWPELVRKDGDFAAAVIERENRRVDAVVILVGSPATADFRCDRVRVWVDRNGIVVTVPV 119

[Close]

BLAST of Moc05g00540 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 73.6 bits (179), Expect = 1.2e-13
Identity = 36/64 (56.25%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GKSSWP+L+G+ G  AK VIERENP+++AVII  G+   + F C RV VWV + GIV + 
Sbjct: 36  GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 113 PTIG 117
           P IG
Sbjct: 96  PIIG 99

[Close]

BLAST of Moc05g00540 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 71.2 bits (173), Expect = 6.0e-13
Identity = 35/63 (55.56%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 54  KSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARPP 113
           K+SWP+L G+ G  A  VIERENP V A +I  GS  T DFRCDRVRV+V    IV + P
Sbjct: 8   KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

Query: 114 TIG 117
             G
Sbjct: 68  KSG 70

[Close]

BLAST of Moc05g00540 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 65.9 bits (159), Expect = 2.5e-11
Identity = 31/64 (48.44%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GK+SWP+L+G+ G  A +VI+ EN  +  V++  G+  T+D  C RVRVWV E  IV R 
Sbjct: 10  GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 69

Query: 113 PTIG 117
           PT G
Sbjct: 70  PTAG 73

[Close]

BLAST of Moc05g00540 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 65.9 bits (159), Expect = 2.5e-11
Identity = 31/64 (48.44%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 53  GKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARP 112
           GK+SWP+L+G+ G  A +VI+ EN  +  V++  G+  T+D  C RVRVWV E  IV R 
Sbjct: 25  GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 84

Query: 113 PTIG 117
           PT G
Sbjct: 85  PTAG 88

[Close]

BLAST of Moc05g00540 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 61.6 bits (148), Expect = 4.7e-10
Identity = 29/63 (46.03%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 54  KSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVARPP 113
           K  WP+LVG  G  AK  IERENP V A++++ G    ++F C+RV V++   G VA  P
Sbjct: 23  KYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAP 82

Query: 114 TIG 117
            IG
Sbjct: 83  MIG 85

The following BLAST results are available for this feature:

BLAST of Moc05g00540 vs. NCBI nr


Match Name	E-value	Identity	Description
1VBW_A	5.2e-27	100.00	Crystal Structure of Bitter Gourd Trypsin Inhibitor [Momordica charantia] >prf\|\|...	[more]
BAB32588.1	5.2e-27	100.00	inhibitor against trypsin, partial [Momordica charantia]	[more]
P24076.1	2.7e-23	89.06	RecName: Full=Glu S.griseus protease inhibitor; AltName: Full=BGIA [Momordica ch...	[more]
KVF69647.1	5.6e-21	51.72	Proteinase inhibitor I13, potato inhibitor I [Cynara cardunculus var. scolymus]	[more]
PQP96738.1	1.4e-19	44.09	Proteinase inhibitor [Prunus yedoensis var. nudiflora]	[more]

BLAST of Moc05g00540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24076	3.5e-26	89.06	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P19873	1.2e-18	64.06	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P80211	7.6e-13	53.73	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
P82381	7.6e-13	53.97	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
P09407	6.5e-12	51.11	Trypsin inhibitor MCI-3 OS=Momordica charantia OX=3673 PE=1 SV=1	[more]

BLAST of Moc05g00540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q9AYN0	2.5e-27	100.00	Inhibitor against trypsin (Fragment) OS=Momordica charantia OX=3673 GN=bgit PE=2...	[more]
Q7M1Q1	2.5e-27	100.00	Trypsin inhibitor BGIT OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
A0A103L5L1	2.7e-21	51.72	Proteinase inhibitor I13, potato inhibitor I OS=Cynara cardunculus var. scolymus...	[more]
A0A314XVX5	6.7e-20	44.09	Proteinase inhibitor OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_08087 ...	[more]
A0A6D2KVQ0	1.9e-19	50.00	Uncharacterized protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS39306 ...	[more]

BLAST of Moc05g00540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43580.1	1.2e-13	56.25	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38870.1	6.0e-13	55.56	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.1	2.5e-11	48.44	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.2	2.5e-11	48.44	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	4.7e-10	46.03	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 93..103 score: 60.47 coord: 56..69 score: 48.45 coord: 81..92 score: 30.43
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 54..116 e-value: 2.7E-24 score: 85.6 coord: 7..42 e-value: 4.6E-9 score: 36.8
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 52..116 coord: 1..44
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 57..68
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..42 e-value: 1.0E-15 score: 59.4
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 49..115 e-value: 1.1E-26 score: 94.9
None	No IPR available	PANTHER	PTHR33091:SF41	SERINE PROTEASE INHIBITOR, POTATO INHIBITOR I-TYPE FAMILY PROTEIN-RELATED	coord: 52..116 coord: 1..44
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 3..42
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 51..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g00540.1	Moc05g00540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity