Moc04g35200 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g35200
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionglycosylphosphatidylinositol anchor attachment 1 protein-like
Locationchr4: 26486601 .. 26490615 (+)
RNA-Seq ExpressionMoc04g35200
SyntenyMoc04g35200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAGACCGAGACACCAAAAGCGAAGGCACGACCGATTGTGCGCCTGGGGATTTTCCTAATTTCTCACAGTGTTTTCTTCAGGTACGCTATTGGTTTTACGTGGATTTGATTCTCAGTTCGTTTCAGAATATATTGATTGATTAATCTGGTTCTTTTTCTTTTTTTGGGTGCTCTCTAGCGTGATTTGCTTCTCCGCAGGGGTTTTAGCTCTTTTGCTACTGCCAATGTTCGCCATGAACACTTACATTTCCGAGAACGCTCTCATGCCAGGTCATTTTTGCTTCTTGCTTAGCTTATCATTATCTTATTTGTTTATTTGTTACTCATCATTTTTATGATTTAGTGATTGATCGCCTGGTGGAGCGTATGAGTTAAGATAATTTACGAATTAAAATTAAAATTCATTAGTTGGGGGAGTATCTTAAATTGTAGGATCATCAATTTTGTGTTCCTTGTCCTTTTGCAGTGATCTATTCTTTGTAATTTTATATATGGATGGACAGTACTCAGAGGTTGTTTAGAGCGTATATATGTTGGAGTACTCTTTAATTGTGGCATTCTATTGTCCCACAGGTTCTGCAAATACCATGCTTTCCGGTCAGGAAGTCTCAGAGGCGAACAACTTGGTGAAGGATATAAAGGCTTTGAACTCGAAACCTGGTTCGACAGTCTTGTATGTAATTAGTGTCCCACTTTTCTCTGTTTCTTTTTTCTCCTTTAAAATACAATGGAAAAATCCTGTGTAATGACTAAAATTTTGAGAATTATCACTTCAAATTAAAATTTGTCTACGTGGTTTGTTATGACTTTGTATGCATTGACATTAATATGGTTTTATGTCGCATGCTTTAAAGGCATTTGCCCATTTTAACTCAGTCAACTCCTTTGACTAATTACAAGTATTTAAGTCAACCAATGGTAGGGTGAAACTTTAGAGTTCACTTGTATGCGTAGTCCCATGAAAATATCAAGGAACCATCACCAAGCTCAATTGTGCCAATTTGGCCAATTGTAAAATATTTAAATATGTCATCCATTTAGCTTTCAATATGAACTTCTGAATGAGGCTGATATTCTTTGTCTAGAAAGGGTTTAGAATATTAGTCCTAAAATTCTTATACAAGTTACGTGTATCAAATGAAAGCTTTCATTAGAATGTGGTAGATGTTATTTCTTCAATAATTTATGAAAGCTTTCATCAGAATGTGGTATATGTTATTTCTTCAATAATTTTCTGAATATGTGATGTCTATGCTTGTTTCATTTTATCTATGACCATGATGTGCATGTGGATTGACATGTTATTTGATGTTTCTCAAGAGGGGCGTGGATGTGTAAAGATAATCATAGTCTTAATTGGTGCAACTTTTTTCACTTCTTTGCAGTGCACTTCATAGTTATCTAATTCATCTTCCACAGAATTTATCAATAGCATATTTTTCACATTTCAATATGCATCATCTTTTTCTTCTCCTTGCCTCTGTTTGCATTGCAAGTCTTTTGTTCTACTCAACCTATGATGAAGAAATTTGATTTTCTATCCATTTTTACAGTGGAAGTCAGCAGATTCTAGCACAATATATATCAGACTTGGGTGCTGAAGTTAATTACCACAAGTTCCACCCTCAGTCTGGTCAGTTCCATCCATTGCACTTTTTCTCTAGCCCTGATTCTGGCATCATTCGGGACAACGTTAGTTGCATGGCACAGGGTATTAACACTGTTGGCATAATAAAAGCACCACAAGCCGATGGAAAGGAAGCAATCGTCTTGGTGACAACTTACAATCCTGTTAAGACTAGTCCTTATGAGACTTTGTCTCTTGGTATTGCATATTCAGTATTTTCTTTGCTTACACAAGTAACTTGGTTGGCCAAAGACGTTGTATGGCTTATCGCAGATTCACAATATGGGGAATATGCTGCAGTTTCAGCCTGGCTTAGAGACTATCACACTCCTGCATTTGGTCGATCCGACATAATTGACAAAGATGCTTGTGCTGGGATGAATGTCCTTAATGAATTAGAAACAAACCATATGGCAGAAAAAATGAGTCTAGATGATTTTAGACGTGCTGGAACAATGGCTGCAGCACTTGTTATTAAGGTTTCAAATAAAAGTGAACACTTCGAGGATAGTCTTAGTGTCTATGCTGAAGCATCTAATGGTCAGATGCCGAACCTGGATCTCATCAATATTGTGAATTATATGGCAGTACATAGGCAAGGTTTTCGGATTAAGATTGAGAAATTTTGGCCTTTACTAGACTGCAAATGGCTCAAGGTTTTGGGTGAAGTGTTTGAGTCAATAGGACAATTGATCCGAATCTTGAACCCTGAATGGAAGTTTGGCATCCCTGCTTCTGACTATGTTGATGGCACTGCCACACTTGCAAGTTCATTGTACTACCAGGTGGTTTACCTTTCTGTTTATTTGTCAAGAGCTATATGGTTTCATTTAATGAACAAGAATAAGGAAGGTTTTTTCATTTATGTTAATAATAAAGATTATATCCATTTAAGTTAATCTATGTTCTTCTTGCCATTAGGCTGTGGGTATTCCTACTGGTCCCCATGGTGCTTTTCGAGATTACCAAGTTGATGCAATTACCGTGGAGATGTCCCCCAAATTTTCTTCTGGTATCAAGGTCAGGCGTGATGAATTCATACTGCGAGGTGGCAGGTCAGTGCTCATTTATACCCCAAGGATTTTGTAACACATCCTCCTCCCACCCCTCAACTTTTCAGGGCTCACTGTATGAATAAATGTTTAAAACTAATGGTTTCCCTCAATGAGGTTAGCTCGACATCAAGTAATATTTTCTATTTTTTTATTATTTATCATTTCCCATTTTTTAACTTCACGTTTTACTGTCAATCTTCCATGTTTAAATTTTAGTTTGTAAATAGTTAAACTGCAGTAAGAGAAAATTTGTTTTTTTAACAATGAACTTTGGCTAATAGTTTCTCTTTTTAAACGAATGGCCAAAGTCATGTTCATGTATGAAACTGGGTTTAGATTTTGGTGTATTAAATGATCAAGATATTCATAATTTTAAATTTGAAATTAAAATGCAAGGACTGAAATTCTCCAAGTCTTTTTAATGGCTAAGGATTTTGTAGTTGGGATTGTGCAGGCTAATTGAAGGAGTTGTACGATCAGTAAACAACCTCCTGGAAAAGTTCCATCAGTCATTCTTCCTATACCTTATGGTATCTACAAGCAAATTTGTATCAGTTGGTGTATATATGATTGCTTTTGCACTCCTTGTTGCTCCACTACCAGCTGTTGCGGCTTCTCTCTACTCTTATGCAAACAATTTGAATTTAACCTCGGAAAAGGTTGAACATGCAGTTCCAGCAAATCCTGATGATGAGCTTATTGTCTCTTTGAGATCATGGAAATGGCTTAATGCTGCAAAAAATGTTTTTGTTGTTCATTTATGGGGTGCTGTTGTTTCTTTACTTCCCTACTTCATCTGCCAAATACCTGGTTACAGTCCTACAACAAGCCCTACTATTTGGGCGTTGCTTTCACTGCTCACCCTACTAATTTTGTCCGTGATTTTGGGCTCACCATTCACTTGCACCAAACCTTCTCACCAGCTTATTCAAGAGTGCGCTTTCCTGAAAGCAATGACCATCTCAGCGGCCTTTATTGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCATTTTTTATGGTTTCCATGTGCTTGATGGCACATCCTCTGAAGCTTGATCTAGTGGCTGGGAGTTTTAAAGCTCTTTCAAGGGCAGCCTGTAACATTGTTTTGGGATTCATAGCTTTTCCACCTATTGCTTTCTTTTTGTTTAAAGGTTCTTTGCAAGGTTTTCATGATCTACATATTGGCGACTTCTGGAACTGGATGGAGACCCTCTGGGCTTGGAATAGTGCTACTTTCCTCTACTTAGGTATGGTCCATCTGCCATGCTGGGCATTATGCATACAAATTTTACTTCATCCTTGTTGA

mRNA sequence

ATGGCTGAGACCGAGACACCAAAAGCGAAGGCACGACCGATTGTGCGCCTGGGGATTTTCCTAATTTCTCACAGTGTTTTCTTCAGCGTGATTTGCTTCTCCGCAGGGGTTTTAGCTCTTTTGCTACTGCCAATGTTCGCCATGAACACTTACATTTCCGAGAACGCTCTCATGCCAGGTTCTGCAAATACCATGCTTTCCGGTCAGGAAGTCTCAGAGGCGAACAACTTGGTGAAGGATATAAAGGCTTTGAACTCGAAACCTGGTTCGACAGTCTTTGGAAGTCAGCAGATTCTAGCACAATATATATCAGACTTGGGTGCTGAAGTTAATTACCACAAGTTCCACCCTCAGTCTGGTCAGTTCCATCCATTGCACTTTTTCTCTAGCCCTGATTCTGGCATCATTCGGGACAACGTTAGTTGCATGGCACAGGGTATTAACACTGTTGGCATAATAAAAGCACCACAAGCCGATGGAAAGGAAGCAATCGTCTTGGTGACAACTTACAATCCTGTTAAGACTAGTCCTTATGAGACTTTGTCTCTTGGTATTGCATATTCAGTATTTTCTTTGCTTACACAAGTAACTTGGTTGGCCAAAGACGTTGTATGGCTTATCGCAGATTCACAATATGGGGAATATGCTGCAGTTTCAGCCTGGCTTAGAGACTATCACACTCCTGCATTTGGTCGATCCGACATAATTGACAAAGATGCTTGTGCTGGGATGAATGTCCTTAATGAATTAGAAACAAACCATATGGCAGAAAAAATGAGTCTAGATGATTTTAGACGTGCTGGAACAATGGCTGCAGCACTTGTTATTAAGGTTTCAAATAAAAGTGAACACTTCGAGGATAGTCTTAGTGTCTATGCTGAAGCATCTAATGGTCAGATGCCGAACCTGGATCTCATCAATATTGTGAATTATATGGCAGTACATAGGCAAGGTTTTCGGATTAAGATTGAGAAATTTTGGCCTTTACTAGACTGCAAATGGCTCAAGGTTTTGGGTGAAGTGTTTGAGTCAATAGGACAATTGATCCGAATCTTGAACCCTGAATGGAAGTTTGGCATCCCTGCTTCTGACTATGTTGATGGCACTGCCACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGTCCCCATGGTGCTTTTCGAGATTACCAAGTTGATGCAATTACCGTGGAGATGTCCCCCAAATTTTCTTCTGGTATCAAGGTCAGGCGTGATGAATTCATACTGCGAGGTGGCAGGCTAATTGAAGGAGTTGTACGATCAGTAAACAACCTCCTGGAAAAGTTCCATCAGTCATTCTTCCTATACCTTATGGTATCTACAAGCAAATTTGTATCAGTTGGTGTATATATGATTGCTTTTGCACTCCTTGTTGCTCCACTACCAGCTGTTGCGGCTTCTCTCTACTCTTATGCAAACAATTTGAATTTAACCTCGGAAAAGGTTGAACATGCAGTTCCAGCAAATCCTGATGATGAGCTTATTGTCTCTTTGAGATCATGGAAATGGCTTAATGCTGCAAAAAATGTTTTTGTTGTTCATTTATGGGGTGCTGTTGTTTCTTTACTTCCCTACTTCATCTGCCAAATACCTGGTTACAGTCCTACAACAAGCCCTACTATTTGGGCGTTGCTTTCACTGCTCACCCTACTAATTTTGTCCGTGATTTTGGGCTCACCATTCACTTGCACCAAACCTTCTCACCAGCTTATTCAAGAGTGCGCTTTCCTGAAAGCAATGACCATCTCAGCGGCCTTTATTGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCATTTTTTATGGTTTCCATGTGCTTGATGGCACATCCTCTGAAGCTTGATCTAGTGGCTGGGAGTTTTAAAGCTCTTTCAAGGGCAGCCTGTAACATTGTTTTGGGATTCATAGCTTTTCCACCTATTGCTTTCTTTTTGTTTAAAGGTTCTTTGCAAGGTTTTCATGATCTACATATTGGCGACTTCTGGAACTGGATGGAGACCCTCTGGGCTTGGAATAGTGCTACTTTCCTCTACTTAGGTATGGTCCATCTGCCATGCTGGGCATTATGCATACAAATTTTACTTCATCCTTGTTGA

Coding sequence (CDS)

ATGGCTGAGACCGAGACACCAAAAGCGAAGGCACGACCGATTGTGCGCCTGGGGATTTTCCTAATTTCTCACAGTGTTTTCTTCAGCGTGATTTGCTTCTCCGCAGGGGTTTTAGCTCTTTTGCTACTGCCAATGTTCGCCATGAACACTTACATTTCCGAGAACGCTCTCATGCCAGGTTCTGCAAATACCATGCTTTCCGGTCAGGAAGTCTCAGAGGCGAACAACTTGGTGAAGGATATAAAGGCTTTGAACTCGAAACCTGGTTCGACAGTCTTTGGAAGTCAGCAGATTCTAGCACAATATATATCAGACTTGGGTGCTGAAGTTAATTACCACAAGTTCCACCCTCAGTCTGGTCAGTTCCATCCATTGCACTTTTTCTCTAGCCCTGATTCTGGCATCATTCGGGACAACGTTAGTTGCATGGCACAGGGTATTAACACTGTTGGCATAATAAAAGCACCACAAGCCGATGGAAAGGAAGCAATCGTCTTGGTGACAACTTACAATCCTGTTAAGACTAGTCCTTATGAGACTTTGTCTCTTGGTATTGCATATTCAGTATTTTCTTTGCTTACACAAGTAACTTGGTTGGCCAAAGACGTTGTATGGCTTATCGCAGATTCACAATATGGGGAATATGCTGCAGTTTCAGCCTGGCTTAGAGACTATCACACTCCTGCATTTGGTCGATCCGACATAATTGACAAAGATGCTTGTGCTGGGATGAATGTCCTTAATGAATTAGAAACAAACCATATGGCAGAAAAAATGAGTCTAGATGATTTTAGACGTGCTGGAACAATGGCTGCAGCACTTGTTATTAAGGTTTCAAATAAAAGTGAACACTTCGAGGATAGTCTTAGTGTCTATGCTGAAGCATCTAATGGTCAGATGCCGAACCTGGATCTCATCAATATTGTGAATTATATGGCAGTACATAGGCAAGGTTTTCGGATTAAGATTGAGAAATTTTGGCCTTTACTAGACTGCAAATGGCTCAAGGTTTTGGGTGAAGTGTTTGAGTCAATAGGACAATTGATCCGAATCTTGAACCCTGAATGGAAGTTTGGCATCCCTGCTTCTGACTATGTTGATGGCACTGCCACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGTCCCCATGGTGCTTTTCGAGATTACCAAGTTGATGCAATTACCGTGGAGATGTCCCCCAAATTTTCTTCTGGTATCAAGGTCAGGCGTGATGAATTCATACTGCGAGGTGGCAGGCTAATTGAAGGAGTTGTACGATCAGTAAACAACCTCCTGGAAAAGTTCCATCAGTCATTCTTCCTATACCTTATGGTATCTACAAGCAAATTTGTATCAGTTGGTGTATATATGATTGCTTTTGCACTCCTTGTTGCTCCACTACCAGCTGTTGCGGCTTCTCTCTACTCTTATGCAAACAATTTGAATTTAACCTCGGAAAAGGTTGAACATGCAGTTCCAGCAAATCCTGATGATGAGCTTATTGTCTCTTTGAGATCATGGAAATGGCTTAATGCTGCAAAAAATGTTTTTGTTGTTCATTTATGGGGTGCTGTTGTTTCTTTACTTCCCTACTTCATCTGCCAAATACCTGGTTACAGTCCTACAACAAGCCCTACTATTTGGGCGTTGCTTTCACTGCTCACCCTACTAATTTTGTCCGTGATTTTGGGCTCACCATTCACTTGCACCAAACCTTCTCACCAGCTTATTCAAGAGTGCGCTTTCCTGAAAGCAATGACCATCTCAGCGGCCTTTATTGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCATTTTTTATGGTTTCCATGTGCTTGATGGCACATCCTCTGAAGCTTGATCTAGTGGCTGGGAGTTTTAAAGCTCTTTCAAGGGCAGCCTGTAACATTGTTTTGGGATTCATAGCTTTTCCACCTATTGCTTTCTTTTTGTTTAAAGGTTCTTTGCAAGGTTTTCATGATCTACATATTGGCGACTTCTGGAACTGGATGGAGACCCTCTGGGCTTGGAATAGTGCTACTTTCCTCTACTTAGGTATGGTCCATCTGCCATGCTGGGCATTATGCATACAAATTTTACTTCATCCTTGTTGA

Protein sequence

MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSGQFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYETLSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDACAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGIPASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQIPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGLCLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFFLFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC
Homology
BLAST of Moc04g35200 vs. NCBI nr
Match: XP_022133688.1 (LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-like [Momordica charantia])

HSP 1 Score: 1372.5 bits (3551), Expect = 0.0e+00
Identity = 692/714 (96.92%), Postives = 699/714 (97.90%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG
Sbjct: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTS YET
Sbjct: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSXYET 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYSVFSLLT VTWLAKD+VWLI DSQYGEYAAVSAWLRDY+TPAFGRS IIDKDA
Sbjct: 181 LSLGIAYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYYTPAFGRSGIIDKDA 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           CA M+VLN+L TNHM EKMSLD+F+RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAEMSVLNKLGTNHMTEKMSLDEFKRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI
Sbjct: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
           PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL
Sbjct: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLT EKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVH WGAVVSLLPYFIC 
Sbjct: 481 SYANNLNLTXEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHXWGAVVSLLPYFICX 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           +PGYSPTTS TIWALLSLL LLI SVILG PFTCTKP HQLIQECAFLKAMTISAAFIGL
Sbjct: 541 JPGYSPTTSXTIWALLSLLXLLIXSVILGXPFTCTKPXHQLIQECAFLKAMTISAAFIGL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF
Sbjct: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC
Sbjct: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 714

BLAST of Moc04g35200 vs. NCBI nr
Match: XP_022144229.1 (LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-like [Momordica charantia])

HSP 1 Score: 1316.2 bits (3405), Expect = 0.0e+00
Identity = 671/714 (93.98%), Postives = 682/714 (95.52%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETETPK KARPIVRLGIFLIS SVFFSVICFS G  ALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETETPKTKARPIVRLGIFLISQSVFFSVICFSTGFXALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SANTMLSGQEVSEANNLVKD+KALNSKPGSTVFG QQILAQYISDLGAEVNYHKFHPQSG
Sbjct: 61  SANTMLSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSG 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           QFHPLHFFSSPDSGIIRDNVSCMA GINTVGII+APQADGKEAIVLVTTYNPVKTS YET
Sbjct: 121 QFHPLHFFSSPDSGIIRDNVSCMAXGINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYET 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYSVFSLLT VTWLAKD+VWLI DSQYGEYAAVSAWLRDY+TPAFGRS IIDKDA
Sbjct: 181 LSLGIAYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYYTPAFGRSGIIDKDA 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           CA M+VLN+L TNHM EKMSLD+F+RAGTMAAALVIKVSNKSE FEDSLSVYAEASNGQM
Sbjct: 241 CAEMSVLNKLGTNHMTEKMSLDEFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLIN VNYMAVHRQGF IK+EKF PLLDCKWLKVLGEVFESIGQLIR LN EWKFGI
Sbjct: 301 PNLDLINXVNYMAVHRQGFWIKVEKFLPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGI 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
            ASDYVDG ATLASSLYYQAVGIPTGPHGAFRDYQVDAIT+EMSPKFSSGIKVRRDEF L
Sbjct: 361 LASDYVDGIATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRRDEFXL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLT EKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ
Sbjct: 481 SYANNLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL
Sbjct: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINFATAELGAFFMVSMCLMAHPLKLDL+AGSFKALSRAACNIVLG IAFPPIAFF
Sbjct: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFKGSLQ       GDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC
Sbjct: 661 LFKGSLQVSTIYIXGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 714

BLAST of Moc04g35200 vs. NCBI nr
Match: XP_023550991.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1258.8 bits (3256), Expect = 0.0e+00
Identity = 631/714 (88.38%), Postives = 667/714 (93.42%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETE PK+KARPIVRLGIFLISHS+FFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SANTMLSGQEVSEAN+LVKD+K LNSK GST+FGSQQILAQYIS+LGAEVNYH+F PQS 
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           QFHPLHFFSSPDSGI++DNVSCM+QGINTVGII+APQADGKEAIVLVT YNPVKTS +ET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYS+FSLLTQVTWLAKD+VWL ADS+YGEYAAVSAWLRDYHTP+FGRS +ID   
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           CA  NVL E E NH+ EK SLDDF+RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLINIVNY+A HRQGFRIKIEKFWPLLDCKWLK LGE FESIG+ IR LNPEWKFG+
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
            ASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQ+DAITVEMSPKFSSG+KVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLT EK+E A PANPDD LIVSLRSWKWLNAAK VFVVHLWGAVVSLLPYFICQ
Sbjct: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           IPGYSPTT+  IWALLSLLTLLILSVILGSP + TK   Q IQE AFLKAMT SAAFIGL
Sbjct: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINFATAELGAF +VSMCL+AHPLKLDLVAG+FKALSRAA N+VLGFIAFPP+ FF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFK +LQGF +LH+GDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Moc04g35200 vs. NCBI nr
Match: XP_038891214.1 (glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Benincasa hispida])

HSP 1 Score: 1253.0 bits (3241), Expect = 0.0e+00
Identity = 625/714 (87.54%), Postives = 661/714 (92.58%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETE PK KARPIVRLGIFLISHS+FFSVICFSAGVLALLLLPMF+MNTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFSMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SANTMLSG EVSEANNLVKD+K LNSKPGS +FGSQQILAQYIS LGA+VNYH+FHPQS 
Sbjct: 61  SANTMLSGWEVSEANNLVKDLKGLNSKPGSAIFGSQQILAQYISKLGADVNYHRFHPQSS 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           QFHPLHFFSSPDSGI+R NVSCM QGINTVGII+APQADGKEAIVLVT YNPVKTS ++T
Sbjct: 121 QFHPLHFFSSPDSGIVRGNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHDT 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYS+FSLLTQVTWLAKD+VWL+ADS+YGEY AVSAWLRDYH P FGRS +ID DA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYDAVSAWLRDYHAPVFGRSSVIDTDA 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           C   NVL E+E NH  EK++LDDF+RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQM
Sbjct: 241 CTETNVLYEVEANHATEKINLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLINIVNY+AVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIG++IR LN EWKFG+
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKIIRSLNSEWKFGM 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
           PASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQ+DAITVEMSPKFSS +KVRRDEFIL
Sbjct: 361 PASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSAVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAA+LY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANN+NLT EKVE   PANPD+ELIVSLRSWKWLNAAK VFVVHLWGAVVSLLPYFICQ
Sbjct: 481 SYANNMNLTLEKVEPPAPANPDNELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           IPGYSPT +  IWALLSLL LLILSVILGSP   TK   Q IQE AFLKAMT S AFI L
Sbjct: 541 IPGYSPTANSIIWALLSLLALLILSVILGSPLGSTKSYEQRIQEWAFLKAMTTSTAFISL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINFATAELGAF +VSMCL+AHPLKLDL+AGSFKALSRAACNIVLGFIAFPP+ FF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLMAGSFKALSRAACNIVLGFIAFPPVTFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFK +LQGF +LHIGDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHPC
Sbjct: 661 LFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Moc04g35200 vs. NCBI nr
Match: KAG7016207.1 (Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1252.7 bits (3240), Expect = 0.0e+00
Identity = 625/714 (87.54%), Postives = 666/714 (93.28%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETE PK KARPIVRLGIFLISHS+FFSVICFSAG+LALLLLPMFA+NTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGILALLLLPMFALNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SANTMLSGQEVSEAN+LVKD+K LNSK GST+FGSQQILAQYIS+LGAEVNYH+F PQS 
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           QFHPLHFFSSPDSGI++DNVSCM QGINTVGII+APQADGKEAIVLVT YNPVKTS +ET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYS+FSLLTQVTWLAKD+VWL ADS+YGEYAAVSAWLRDYHTP+FGRS++ID   
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           CA  NVL E E NH+ EK SLDDF+RAGTMAAAL+IKVSN+SEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALIIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLINIVNY+A HRQGFRIKIEKFWPLLDCKWLK LGE+FE+IG+ IR LNPEWKFG+
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
            ASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQ+DAITVEMSPKFSSG+KVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLT EK+E A PANPDDELIVSLRSW+WLNAAK VFVVHLWGAVVSLLPYFICQ
Sbjct: 481 SYANNLNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           IP YSPTT+  IWALLSLLTLLILSVILGSP + TK   Q IQE AFLKAMT SAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINFATAELGAF +VSMCL+AHPLKLDLVA +FKALSRAA N+VLGFIAFPP+ FF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAWNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFK +LQGF +LH+GDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Moc04g35200 vs. ExPASy Swiss-Prot
Match: Q9WTK3 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=10090 GN=Gpaa1 PE=1 SV=3)

HSP 1 Score: 114.4 bits (285), Expect = 5.3e-24
Identity = 158/631 (25.04%), Postives = 262/631 (41.52%), Query Frame = 0

Query: 8   KAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLL--PMFAMNTYISENALMPGSANTM 67
           +A AR ++RL   L        V+ + AG+   L L  P     TY+SENA+     +TM
Sbjct: 11  RALARIVLRLNTPL-------CVLSYVAGIAWFLALAFPPLTQRTYMSENAM----GSTM 70

Query: 68  LSGQEV--SEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSGQFH 127
           +  Q V    A +  +D  A   KPG+        L + +  +G EV    F  +     
Sbjct: 71  VEEQFVGGDRARSFARDFAAHRKKPGALPVA---WLERSMRSVGLEVYTQSFSRK----- 130

Query: 128 PLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYETLSL 187
                  PD      +   M  G N  GI++AP++   E++VL     P  T+   + ++
Sbjct: 131 ----LPFPD----ETHERYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATN---SQAV 190

Query: 188 GIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDACAG 247
           G+  ++ +      + AKD+++L+ D    +     AWL  YH                 
Sbjct: 191 GLLLALAAHFRGQIYWAKDIIFLVTDH---DLLGTEAWLEAYH----------------D 250

Query: 248 MNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNL 307
           +NV     T   +  +      RAG + AA+ +++S+       SL V  E  NGQ+PNL
Sbjct: 251 INV-----TGIQSSPLQ----GRAGAIQAAVALELSS---DVVTSLDVTVEGLNGQLPNL 310

Query: 308 DLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGIPAS 367
           DL+N+       + G    ++    L    W  + G                        
Sbjct: 311 DLLNLFQTFC-QKGGLLCTLQ--GKLQPQDWTSLEGP----------------------- 370

Query: 368 DYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFILRG- 427
             + G  TL   +  QA G P GPHG F  Y V+A+T+     F      R+ ++ L   
Sbjct: 371 --LQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSF------RQYKYDLATV 430

Query: 428 GRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSY 487
           G+ +EG+ R +N+LLE+ HQSFF YL+ + S+FVS+G+YM A   L+  L   A  L+  
Sbjct: 431 GKALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQ 490

Query: 488 ANNLNLTSEKVEHA-VPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQI 547
            +   +  E+   A  P  P    ++  +     +    + +    G  +  LP     +
Sbjct: 491 LHQAGVNPEEAGKAPSPGTP----LLPTQGVGLASLTAPLLISQAMGLALYFLP-----V 537

Query: 548 PGYSPTTS--PTIWALLSLLTLLILSVI-LGSPFTCTKPSHQLIQECAFLKAMTISAAFI 607
            G    T   P   A   +LTLL + V  L  P    +  +  + +  ++    ++  ++
Sbjct: 551 LGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPHNTHRVVNSQVPDRGWMALKLVALIYL 537

Query: 608 GLCL--MSVINFATAELGAFFMVSMCLMAHP 628
            L L  ++++NF+   L A  MV    +A P
Sbjct: 611 ALQLGCIALLNFSLGFLLAATMVPAAALAKP 537

BLAST of Moc04g35200 vs. ExPASy Swiss-Prot
Match: O43292 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606 GN=GPAA1 PE=1 SV=3)

HSP 1 Score: 110.5 bits (275), Expect = 7.7e-23
Identity = 125/471 (26.54%), Postives = 199/471 (42.25%), Query Frame = 0

Query: 8   KAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTMLS 67
           +A AR ++RL   L   S    +  F A     L+ P     TY+SENA+         +
Sbjct: 11  RALARLVLRLNAPLCVLSYVAGIAWFLA-----LVFPPLTQRTYMSENAMGSTMVEEQFA 70

Query: 68  GQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSGQFHPLHF 127
           G +   A    +D  A   K G+        L + +  +G EV    F  +         
Sbjct: 71  GGD--RARAFARDFAAHRKKSGALPVA---WLERTMRSVGLEVYTQSFSRK--------- 130

Query: 128 FSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYETLSLGIAY 187
              PD      +   M  G N  GI++AP+A   E++VL     P  +    + ++G+  
Sbjct: 131 LPFPD----ETHERYMVSGTNVYGILRAPRAASTESLVLTV---PCGSDSTNSQAVGLLL 190

Query: 188 SVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDACAGMNVL 247
           ++ +      + AKD+V+L+ +    +     AWL  YH                 +NV 
Sbjct: 191 ALAAHFRGQIYWAKDIVFLVTEH---DLLGTEAWLEAYH----------------DVNV- 250

Query: 248 NELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLIN 307
               T   +  +      RAG + AA+ +++S+       SL V  E  NGQ+PNLDL+N
Sbjct: 251 ----TGMQSSPLQ----GRAGAIQAAVALELSS---DVVTSLDVAVEGLNGQLPNLDLLN 310

Query: 308 IVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGIPASDYVD 367
           +       + G    ++    L    W  + G                          + 
Sbjct: 311 LFQTFC-QKGGLLCTLQ--GKLQPEDWTSLDGP-------------------------LQ 370

Query: 368 GTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEF-ILRGGRLI 427
           G  TL   +  QA G P G HG F  Y+V+A+T+     F      R+ ++ ++  G+ +
Sbjct: 371 GLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSF------RQYKYDLVAVGKAL 390

Query: 428 EGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYM--IAFALLVAPLPAV 476
           EG+ R +N+LLE+ HQSFFLYL+   S+FVS+G+YM  + F LLV  L A+
Sbjct: 431 EGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKAL 390

BLAST of Moc04g35200 vs. ExPASy Swiss-Prot
Match: P39012 (GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GAA1 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.2e-15
Identity = 117/497 (23.54%), Postives = 207/497 (41.65%), Query Frame = 0

Query: 12  RPIVRLGIF--LISHSVFFSVICFSAGVLALLLLPMFAM--NTYISENALMPGSANTMLS 71
           R IV +G+   +I+     S++C   G +++ +LPM      TYISENALMP  A +   
Sbjct: 9   RRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMPSQAYSYFR 68

Query: 72  GQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSGQFHPLHF 131
             E +        IK +      T      ++  ++ + G +   +    ++ Q+     
Sbjct: 69  ESEWNILRGYRSQIKEM---VNMTSMERNNLMGSWLQEFGTKTAIY----ENEQY----- 128

Query: 132 FSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVL-VTTYNPVKTSPYETLSLGIA 191
                             G    G++ AP+ DG EA+VL V  +N          +LG++
Sbjct: 129 ------------------GETLYGVMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAALGVS 188

Query: 192 YSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDACAGMNV 251
            + F     V W +K+++ + +++     AA+ +W+  YHT                   
Sbjct: 189 LARFFSRWPV-W-SKNIIVVFSEN---PRAALRSWVEAYHT------------------- 248

Query: 252 LNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLI 311
                        SLD     G++ AA+V+  S+  + FE  + +  +  NG++PNLDL+
Sbjct: 249 -------------SLD--LTGGSIEAAVVLDYSSTEDFFE-YVEISYDGLNGELPNLDLV 308

Query: 312 NIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGIPASDYV 371
           NI   +  H +G ++ +                                   G+P+    
Sbjct: 309 NIAISITEH-EGMKVSLH----------------------------------GLPSDQLT 368

Query: 372 DGT-----ATLASSLYYQAVGIPTGPHG--AFRDYQVDAITVEMSPKFSSGIKVRRDEFI 431
           +         L   +   A+     PHG  AF  +++ ++T++     +SG        I
Sbjct: 369 NNNFWSRLKILCLGIRDWALSGVKKPHGNEAFSGWRIQSVTLKAHG--NSG------HDI 389

Query: 432 LRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYM-----IAFALLVAPLPA 491
              GR+ E + RS+NNLLEKFHQSFF YL+++  +FVS+  Y+     ++ A  ++ L A
Sbjct: 429 TTFGRIPEAMFRSINNLLEKFHQSFFFYLLLAPRQFVSISSYLPSAVALSIAFAISSLNA 389

BLAST of Moc04g35200 vs. ExPASy Swiss-Prot
Match: Q9US48 (GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gaa1 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 2.7e-15
Identity = 153/683 (22.40%), Postives = 261/683 (38.21%), Query Frame = 0

Query: 15  VRLGIFLISHSVFFSVICFSAGVLALLLLPMFAM--NTYISENALMPGSANTMLSGQEVS 74
           +R+  FL  H  F  +     G+  + +LP   +    ++SE+AL+PG  NT    +   
Sbjct: 9   IRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRY-- 68

Query: 75  EANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSGQFHPLHFFSSPD 134
                            S    S    A   S L A V  +  +    Q        +  
Sbjct: 69  -----------------SKTVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQK 128

Query: 135 SGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYETLSLGIAYSVFSL 194
                +       G N +  ++AP+ D  E+++L   +       Y    + +A S+   
Sbjct: 129 QNYSINIPGSEFNGSNFITTLRAPRGDATESLLLCVPWKD-HIGQYNEAGVALAISLLKY 188

Query: 195 LTQVTWLAKDVVWLIADSQ-YGEYAAVSAWLRDYHTPAFGRSDIIDKDACAGMNVLNELE 254
               +  +KD++ +I D   YG  + ++++  D  TP    + +                
Sbjct: 189 FQGWSLWSKDIILVIFDDPVYGPSSFLTSYF-DQTTPYISYTPL---------------- 248

Query: 255 TNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDLINIVNY 314
                 K+      R+G++ A L +++   +E+  D L V  +A+NGQ+PNLDL N ++ 
Sbjct: 249 ------KI------RSGSIQAGLSLELVT-TENNSDVLEVLYQATNGQLPNLDLFNTIS- 308

Query: 315 MAVHRQGFRIKIEKF-WPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGIPASDYVDGTA 374
                   RI ++ F +P      L++ G  F +                  S Y     
Sbjct: 309 --------RIFMQHFNYP------LRLQGYDFHANS---------------GSSYTSRLK 368

Query: 375 TLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEF---ILRGGRLIE 434
           +L   +  QAV   T  H  F  Y++D +T+ M  K         D F   + R G+ IE
Sbjct: 369 SLWMGMLTQAVSNVTSAHALFPQYRIDMLTLRMKVK---------DPFSFDMFRFGQAIE 428

Query: 435 GVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLN 494
              RS+NNLLE  HQSFF Y ++    F+S+G YM +  +L     A +  L +Y +   
Sbjct: 429 STFRSLNNLLEHLHQSFFFYFILDHLHFISIGNYMPSILIL-----AASFMLGAYRH--- 488

Query: 495 LTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQIPGYSPT 554
                                     W+N  K    + LW      L    C I  Y   
Sbjct: 489 --------------------------WINHEKK---IDLWRPFSFWLFSIFCTIAAYYLV 543

Query: 555 TSPT-IWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGLCLMSVI 614
           +S T I   + L  +L    I+ S F  ++ +  ++      K++ IS       ++S +
Sbjct: 549 SSSTKITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFIS-------VVSTL 543

Query: 615 NFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFFLFKGSL 674
           NF+ +     F+V++ L+  PL+      SF+   R    + L F      + F+F  SL
Sbjct: 609 NFSLS-----FVVAILLV--PLQFI----SFRFNRR----LSLLFAVLTYFSTFIFLCSL 543

Query: 675 QGFHDLHIGDFWNWMETLWAWNS 690
               +  +  FW W +    +NS
Sbjct: 669 SKILNGPLVPFWLWAKEYELFNS 543

BLAST of Moc04g35200 vs. ExPASy TrEMBL
Match: A0A6J1BZU7 (LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-like OS=Momordica charantia OX=3673 GN=LOC111006214 PE=4 SV=1)

HSP 1 Score: 1372.1 bits (3550), Expect = 0.0e+00
Identity = 692/714 (96.92%), Postives = 699/714 (97.90%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG
Sbjct: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTS YET
Sbjct: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSXYET 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYSVFSLLT VTWLAKD+VWLI DSQYGEYAAVSAWLRDY+TPAFGRS IIDKDA
Sbjct: 181 LSLGIAYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYYTPAFGRSGIIDKDA 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           CA M+VLN+L TNHM EKMSLD+F+RAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAEMSVLNKLGTNHMTEKMSLDEFKRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI
Sbjct: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
           PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL
Sbjct: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLT EKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVH WGAVVSLLPYFIC 
Sbjct: 481 SYANNLNLTXEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHXWGAVVSLLPYFICX 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           +PGYSPTTS TIWALLSLL LLI SVILG PFTCTKP HQLIQECAFLKAMTISAAFIGL
Sbjct: 541 LPGYSPTTSXTIWALLSLLXLLIXSVILGXPFTCTKPXHQLIQECAFLKAMTISAAFIGL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF
Sbjct: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC
Sbjct: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 714

BLAST of Moc04g35200 vs. ExPASy TrEMBL
Match: A0A6J1CRQ0 (LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-like OS=Momordica charantia OX=3673 GN=LOC111013966 PE=4 SV=1)

HSP 1 Score: 1316.2 bits (3405), Expect = 0.0e+00
Identity = 671/714 (93.98%), Postives = 682/714 (95.52%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETETPK KARPIVRLGIFLIS SVFFSVICFS G  ALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETETPKTKARPIVRLGIFLISQSVFFSVICFSTGFXALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SANTMLSGQEVSEANNLVKD+KALNSKPGSTVFG QQILAQYISDLGAEVNYHKFHPQSG
Sbjct: 61  SANTMLSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSG 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           QFHPLHFFSSPDSGIIRDNVSCMA GINTVGII+APQADGKEAIVLVTTYNPVKTS YET
Sbjct: 121 QFHPLHFFSSPDSGIIRDNVSCMAXGINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYET 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYSVFSLLT VTWLAKD+VWLI DSQYGEYAAVSAWLRDY+TPAFGRS IIDKDA
Sbjct: 181 LSLGIAYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYYTPAFGRSGIIDKDA 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           CA M+VLN+L TNHM EKMSLD+F+RAGTMAAALVIKVSNKSE FEDSLSVYAEASNGQM
Sbjct: 241 CAEMSVLNKLGTNHMTEKMSLDEFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLIN VNYMAVHRQGF IK+EKF PLLDCKWLKVLGEVFESIGQLIR LN EWKFGI
Sbjct: 301 PNLDLINXVNYMAVHRQGFWIKVEKFLPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGI 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
            ASDYVDG ATLASSLYYQAVGIPTGPHGAFRDYQVDAIT+EMSPKFSSGIKVRRDEF L
Sbjct: 361 LASDYVDGIATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRRDEFXL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLT EKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ
Sbjct: 481 SYANNLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL
Sbjct: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINFATAELGAFFMVSMCLMAHPLKLDL+AGSFKALSRAACNIVLG IAFPPIAFF
Sbjct: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFKGSLQ       GDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC
Sbjct: 661 LFKGSLQVSTIYIXGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 714

BLAST of Moc04g35200 vs. ExPASy TrEMBL
Match: A0A6J1FHQ4 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445544 PE=4 SV=1)

HSP 1 Score: 1248.4 bits (3229), Expect = 0.0e+00
Identity = 624/714 (87.39%), Postives = 665/714 (93.14%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETE PK+KARPIVRLGIFLISHS+FFSVICFSAGVLALLLLPMFA+NTYISENALMPG
Sbjct: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAINTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SANTMLSGQEVSEAN+LVKD+K LNSK GST+FGSQQILAQYIS+LGAEVNYH+F PQS 
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           +FHPLHFFSSPDSGI++DNVSCM QGINTVGII+APQADGKEAIVLVT YNPVKTS +ET
Sbjct: 121 RFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYS+FSLLTQVTWLAKD+VWL ADS+YGEYAAVSAWLRDYHTP+FGRS +ID   
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           CA  NVL E E NH+ EK SLDDF+RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLINIVNY+A HRQGF+IKIEKFWPLLDCKWLK LGE+FE+IG+ IR LNPEWKFG+
Sbjct: 301 PNLDLINIVNYLAEHRQGFQIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
            ASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQ+DAITVEMSPKFSSG+KVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANN NLT EK+E A PANPDDELIVSLRSW+WLNAAK VFVVHLWGAVVSLLPYFIC 
Sbjct: 481 SYANNSNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICP 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           IP YSPTT+  IWALLSLLTLLILSVILGSP + TK   Q IQE AFLKAMT SAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINFATAELGAF +VSMCL+AHPLKLDLVAG+FKALSRAA N+VLGFIAFPP+ FF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFK +LQGF +LH+GDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Moc04g35200 vs. ExPASy TrEMBL
Match: A0A6J1K1W5 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489371 PE=4 SV=1)

HSP 1 Score: 1247.3 bits (3226), Expect = 0.0e+00
Identity = 627/714 (87.82%), Postives = 663/714 (92.86%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETE PK KARPIVRLGIFLISHS+FFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SANTMLSGQEVSEAN+LVKD+K LNSK GST+FGSQQILAQYIS+LGAEVNYH+F PQS 
Sbjct: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           QFHPLHFFSSPDSGI++DNVSCM QGINTVGII+APQADGKEAIVLVT YNPVKTS +ET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYS+FSLLTQV+WLAKD+VWL ADS+YGEYAAVSAWLRDYHTP+FGRS++ID   
Sbjct: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           CA  NVL E E NH+ EK SLDDF+RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLINIVNY+A HRQGFRIKIEKFWPLLDCKWLK LGE FESIG+ IR LNPEWKFG+
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
            ASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQ+DAITVEMSPKFSSG+KVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANN NLT EK+E A PAN DDELIVSLRSWKWLNAAK VFVVHLWGAVVSLLPYFI Q
Sbjct: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           IPGYSPTT+  IWALLSLLTLLILSVILGSP + TK   Q IQE AFLKAMT SAAFIGL
Sbjct: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINFATAELGAF +VSMCL+AHPLKLDLVAG+FKALSRAA N+VLGFIAFPP+ FF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFK +LQGF +LH+GDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Moc04g35200 vs. ExPASy TrEMBL
Match: A0A0A0KP05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1)

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 620/714 (86.83%), Postives = 667/714 (93.42%), Query Frame = 0

Query: 1   MAETETPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETE  K KARPIVRLGIFLISHS+FFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSG 120
           SAN+MLSG+EVSEANNLVKD+K LNSKPGS++FGSQQILAQYIS LGA+VNYH+FHPQS 
Sbjct: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120

Query: 121 QFHPLHFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYET 180
           QFHPLHFFSSPDSGI++DNVSCM QGINTVGII+APQADGKEAIVLVT YNPV+TS ++T
Sbjct: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDA 240
           LSLGIAYS+FSLLTQVTWLAKD+VWL+ADS+YGEYAAVSAWLRDYHTP FG+S +ID DA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240

Query: 241 CAGMNVLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQM 300
           C+  NVL+E E N + EK  LDDF+RAGTMAAALVIKVSN+SEHFEDSLSVYAEASNGQM
Sbjct: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGI 360
           PNLDLINIVNY+AVHRQGFRIKIEKFWPLL+CKWLKVLGEVFESIG++IR LN EWKFG+
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360

Query: 361 PASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFIL 420
            ASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQ+DAITVEMSPKFSSGIKVRRD+FIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVST KFVSVGVYMIAFALLVAPLPAVAA+LY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLTSEKVE    ANPDDELIVSLRSWKWLNAAK VFVVHLWGA+VSLLPYFICQ
Sbjct: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540

Query: 541 IPGYSPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGL 600
           IPGYSPT +  IW LLSLL+LL+LSVILGSPF+ TK   Q IQE AFLKAMT SAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFF 660
           CLMSVINF+TAELGAF +VSMCL+AHPLKLDL AG+FKALSRAACN+VLGFIAFPP+ FF
Sbjct: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKGSLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHPC 715
           LFKG+LQGF +LHIGDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHPC
Sbjct: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 714

BLAST of Moc04g35200 vs. TAIR 10
Match: AT5G19130.1 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 795.0 bits (2052), Expect = 4.8e-230
Identity = 417/713 (58.49%), Postives = 526/713 (73.77%), Query Frame = 0

Query: 6   TPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTM 65
           +PK K RPIVRLGIFLI+HS  FSV+  +AGVLALLLLP+ A NTYISENALMPGSA +M
Sbjct: 18  SPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSM 77

Query: 66  LSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSGQFHPL 125
           LS ++VS+ + LVKDIK             Q+++ +Y+SD+GAEV+Y KFHP+  QFHPL
Sbjct: 78  LSNRDVSDGSKLVKDIKNFRLNHEGQGVEVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPL 137

Query: 126 HFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYETLSLGI 185
           HFFS PDS  + +NVSC + G+N  GII+AP+ DGKE+IVLVT Y+ +    YE LSLGI
Sbjct: 138 HFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGI 197

Query: 186 AYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDACAGMN 245
             S+FSLL++VTWL+KD++WL+ADS+YG+Y  V+AWL +YH+P+F  SD++  D      
Sbjct: 198 VSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCD------ 257

Query: 246 VLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDL 305
                      E+ + D+FRRAGTMAAALV+KV  +SE FED+LS+YAEASNGQMPNLDL
Sbjct: 258 -----------EQNTADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDL 317

Query: 306 INIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGIPASDY 365
           IN+VNY+AVHRQGF +K+EK   LL   WLK+ GE+FE++G+L  +LNP+W FGIPA+DY
Sbjct: 318 INVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADY 377

Query: 366 VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFILRGGRL 425
           ++G+ATLASSLY QA+GIPTGPHGAFRDYQVDAIT+++SP+F    K R+ +F LRG RL
Sbjct: 378 LEGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARL 437

Query: 426 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 485
           +EG +RSVNNLLEKFHQSFFLY++ S SKF+SVGVYMIAFALLVAPLP VAASLY     
Sbjct: 438 LEGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLY-IDGC 497

Query: 486 LNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQIPG-Y 545
            +LT+         NP +    + +SWKWL+AAK VF +HL G +V+LLPYFICQ+PG +
Sbjct: 498 KSLTNS------THNPTE----NFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQH 557

Query: 546 SPTTSPTIWALLSLLTLLILSVILG--SPFTCTKPSHQLIQECAFLKAMTISAAFIGLCL 605
           SPT    +W   S   LLI  V +   SPF+    S       A LK++TISAAFIGLCL
Sbjct: 558 SPTNRSIMWGTTSSSLLLITFVTMPGCSPFS----SRLHGTNWAILKSVTISAAFIGLCL 617

Query: 606 MSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFFLF 665
           MS+INFATAE+GA  +V  CLMA P+K  L +   K+L  A C+IVL  I FP + F + 
Sbjct: 618 MSIINFATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAIS 677

Query: 666 KGSL-QGFHDLHI-GDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 714
           KG L +G   L + G+FW W+E+LWAW SAT+LY+GMVHLPCW LC+ IL HP
Sbjct: 678 KGLLGEGLVGLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 698

BLAST of Moc04g35200 vs. TAIR 10
Match: AT5G19130.2 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 793.9 bits (2049), Expect = 1.1e-229
Identity = 418/713 (58.63%), Postives = 527/713 (73.91%), Query Frame = 0

Query: 6   TPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTM 65
           +PK K RPIVRLGIFLI+HS  FSV+  +AGVLALLLLP+ A NTYISENALMPGSA +M
Sbjct: 18  SPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSM 77

Query: 66  LSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSGQFHPL 125
           LS ++VS+ + LVKDIK         V   Q+++ +Y+SD+GAEV+Y KFHP+  QFHPL
Sbjct: 78  LSNRDVSDGSKLVKDIKNFRLNHEGQV---QKLIGKYMSDMGAEVSYQKFHPEGNQFHPL 137

Query: 126 HFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSPYETLSLGI 185
           HFFS PDS  + +NVSC + G+N  GII+AP+ DGKE+IVLVT Y+ +    YE LSLGI
Sbjct: 138 HFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGI 197

Query: 186 AYSVFSLLTQVTWLAKDVVWLIADSQYGEYAAVSAWLRDYHTPAFGRSDIIDKDACAGMN 245
             S+FSLL++VTWL+KD++WL+ADS+YG+Y  V+AWL +YH+P+F  SD++  D      
Sbjct: 198 VSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCD------ 257

Query: 246 VLNELETNHMAEKMSLDDFRRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDL 305
                      E+ + D+FRRAGTMAAALV+KV  +SE FED+LS+YAEASNGQMPNLDL
Sbjct: 258 -----------EQNTADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDL 317

Query: 306 INIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGIPASDY 365
           IN+VNY+AVHRQGF +K+EK   LL   WLK+ GE+FE++G+L  +LNP+W FGIPA+DY
Sbjct: 318 INVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADY 377

Query: 366 VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFILRGGRL 425
           ++G+ATLASSLY QA+GIPTGPHGAFRDYQVDAIT+++SP+F    K R+ +F LRG RL
Sbjct: 378 LEGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARL 437

Query: 426 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 485
           +EG +RSVNNLLEKFHQSFFLY++ S SKF+SVGVYMIAFALLVAPLP VAASLY     
Sbjct: 438 LEGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLY-IDGC 497

Query: 486 LNLTSEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQIPG-Y 545
            +LT+         NP +    + +SWKWL+AAK VF +HL G +V+LLPYFICQ+PG +
Sbjct: 498 KSLTNS------THNPTE----NFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQH 557

Query: 546 SPTTSPTIWALLSLLTLLILSVILG--SPFTCTKPSHQLIQECAFLKAMTISAAFIGLCL 605
           SPT    +W   S   LLI  V +   SPF+    S       A LK++TISAAFIGLCL
Sbjct: 558 SPTNRSIMWGTTSSSLLLITFVTMPGCSPFS----SRLHGTNWAILKSVTISAAFIGLCL 617

Query: 606 MSVINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFFLF 665
           MS+INFATAE+GA  +V  CLMA P+K  L +   K+L  A C+IVL  I FP + F + 
Sbjct: 618 MSIINFATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAIS 677

Query: 666 KGSL-QGFHDLHI-GDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 714
           KG L +G   L + G+FW W+E+LWAW SAT+LY+GMVHLPCW LC+ IL HP
Sbjct: 678 KGLLGEGLVGLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133688.10.0e+0096.92LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-li... [more]
XP_022144229.10.0e+0093.98LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-li... [more]
XP_023550991.10.0e+0088.38glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurb... [more]
XP_038891214.10.0e+0087.54glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Benincasa h... [more]
KAG7016207.10.0e+0087.54Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma... [more]
Match NameE-valueIdentityDescription
Q9WTK35.3e-2425.04Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=1009... [more]
O432927.7e-2326.54Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606... [more]
P390121.2e-1523.54GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
Q9US482.7e-1522.40GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC ... [more]
Match NameE-valueIdentityDescription
A0A6J1BZU70.0e+0096.92LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-li... [more]
A0A6J1CRQ00.0e+0093.98LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-li... [more]
A0A6J1FHQ40.0e+0087.39glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A6J1K1W50.0e+0087.82glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A0A0KP050.0e+0086.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G19130.14.8e-23058.49GPI transamidase component family protein / Gaa1-like family protein [more]
AT5G19130.21.1e-22958.63GPI transamidase component family protein / Gaa1-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007246GPI transamidase component Gaa1PIRSFPIRSF036762GAA1coord: 1..714
e-value: 6.1E-198
score: 657.1
IPR007246GPI transamidase component Gaa1PFAMPF04114Gaa1coord: 145..706
e-value: 5.6E-108
score: 362.1
IPR007246GPI transamidase component Gaa1PANTHERPTHR13304GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 1..711
NoneNo IPR availablePANTHERPTHR13304:SF0GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 1..711

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g35200.1Moc04g35200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016255 attachment of GPI anchor to protein
cellular_component GO:0042765 GPI-anchor transamidase complex
cellular_component GO:0016021 integral component of membrane