Moc04g33840 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g33840
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPhenylalanine ammonia-lyase
Locationchr4: 25520287 .. 25522881 (-)
RNA-Seq ExpressionMoc04g33840
SyntenyMoc04g33840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATGTTGGCATGGGGTTGCGGCTCGACCTCAAGTGGAGGCTAGTAGTAAATGGGTTGTCGATAGTCTGAATAAAGGGACCGATAGTTATGGCATAACGACTAGTTTTGGAGCCACATCCCATCGAAGGACCGATCGAGGAGAAACCCTTCAAAAAGAGCTTATTAGGTGAATTTTTTTCTCATTTTCGTCAGCAACGTCAAGTTTAAATTTTCACTCCATATTTAAGATTTTAGGGGATTCGAACTTGCAACCTTTAGAGGTATATGTCAATTACCCTTAAGCTATGGTCGCTTTGACCTATATAATATTTTTATTAAATAATTTACTAAAAATTAATAGCAGTGACTAAAATAGTTTTTTTTTAACCAATATTTATGAATTAAATTAGAACATTCGAAAATTTTGAAACTGAATTAAAAAATATTTAAAATTTCAAAAAGCAAAATAAGATTTAAGACAAAATTGGGCTTACGTTATTGGTAGGGTTTGTCATTGATGATGTGCAAATACTTAGCGAGTTTGGTCAATAAAATTGAACTATTTTACGGCAATTTTTGGTCTCTTTATTATTGCTTTTTTATTTATGGGCGAGTCGGTGACTTTATTTTATTATTATTATTCTTTTGAAAAAGTTGGTGACTTTAACTAATTGCCTATTTTTTTCTGTCATATAATTAATGAATAAGCCTACACGACGACATAAGTGGTTATAACGAACAAATTTATGGTATTCTTCTACTTATAAGTTAAATAGGAGAAATGGTACCTACGTCATTTTTAATACACTGTTGATGTGTCAAGTAATAAATAGAAGAAGTATGTTACTCTAGTAGTACCAGAAAATTTTCCAGCTATAATTATAGTGTAATTATTGTTATTTTTTTAACAAATTATAAAATATTCCGTTCCTAATTAAACAACCACTTATTTATATATTTTTATTTAAATTGTGATATATTTTTAAATAGTATAATATATCGTTTTTGGGATTTTTTTAATAATGTGAATTTTTAAAATATTTACCAAAATATGCCTCATCTCAAACTAATTACAAGAATAATGCTTTGAAATCAGAGGCGACCAACAAACGTGGCGCTTACTCAGCGCCACGGTAATTTTTTTTTTAAAATTAACCATGGTTGAAGAGACGACCATGTCTTTTTTTCTTTATTTTTTTAAAATACTAGGTTTTTTTTTACTTTGGTCGTCTCTGAAGAGACGACCAGAAGAGGTTGCGGCGGCTAGACGGTGGACTGATCGGCGGAGACGGCGGCGGAAAAATCGGCGGCGGCGGCGGCTGAATGTATTTTGGATATGGGGATAGGGGTGAGGGGTTAGGGTTTTTAAAACGAAACCTATTTTAAAAACCCAAGAGACGACCAAGGCGCCAGCTGACGCCCATAAGAGACGACCAAGCGGGATTTGAAAAAATCCCGCCTAAAATGTCGTCTCTTCAAGAGACGACATGTTACGTTATTTTAAAGAAAAAAAGAAAAAAAAGACATGGTCGTCTCTTCAACCATGGTTAATTTTAAAAAAAAAAAATTACAGTGGCGCTGAGTAAGCGCCACGTTTGTTGGTTGTCTCTTCAAGAGACAGACCAACTTATGCCAGCTCATAGGTCGTCTCTTCAAGAGACGACCTATGAATTTCAAAGCATTATTCTTGTAATTAGTTTGAGATGAGGTATATTTTGGTAAATATTTTAAAAATTCACATTATTAAAAAAAAACCCTCGTTTTTGCCCCTCGAAATGTCATAATAAGTTTATATTGTTGTTAAATTTTGAAATTTTAATAATCAATTTCTGAAAAGAAATTTCAAAACTAAATCAATTACTTATGATTTTAAGATTTTAGTTAATATTTAAAGATGTTTTCAAAAATTAAATTAGGAAACAAATATATCATTTTAAAAGAAATTCAATGTTCTTGAAAATACTAAAGAGACAACAACTAAAAATAATTTTGTAATAAGATGGAACTTAAAAAAAAATTATATATATATATAATATATATTTGACCTCTAAAATAAATTTCAACAATAAATGATTGATTGGTAGCATTACTTATTACTAGATAAAGAATTTTTAAGGTTTACTCAAACTTGCAGAGATTAATATGCTTTATTTTCCTAGAAAAAAAAGATAAATGGGTTAGAATTTTATTTTACTTTAATGAATTATTGATGGAGTAATTTGTGTGTGGACAGATTTTTAAACGCTGGAATTTTTGGGAATGATCCAAATTCTCTCAACACATTGCCACAGTCAGCAACACGAGCCGCTATGCTTGTGAGAATCAACACTCTTTTGCAAGGCTATTCGAGGATTAGGTTTGAAATTTTAGAAGCCATAACCAGATTGCTCAACCATTACATAACCCCATGTCTTCCTCTTAGAGGCACAATTACTGCTTCTGGTGACCTCGTCCCTCTCTCTTACATTGTTGCACTTTTGATTGGTAGGCCCAATTCTAGGGCCATTGGACCGAGTGACAAATGTCTCAACCCAAACCAGGCCTTCCATCTCGCAGAGATCAATGATGGATTCTTCCATTTGCAGCCGAAAAGAAGGTCTTGCTCTTGTTAA

mRNA sequence

ATGAAATGTTGGCATGGGGTTGCGGCTCGACCTCAAGTGGAGGCTAGTAGTAAATGGGTTGTCGATAGTCTGAATAAAGGGACCGATAGTTATGGCATAACGACTAGTTTTGGAGCCACATCCCATCGAAGGACCGATCGAGGAGAAACCCTTCAAAAAGAGCTTATTAGATTTTTAAACGCTGGAATTTTTGGGAATGATCCAAATTCTCTCAACACATTGCCACAGTCAGCAACACGAGCCGCTATGCTTGTGAGAATCAACACTCTTTTGCAAGGCTATTCGAGGATTAGGTTTGAAATTTTAGAAGCCATAACCAGATTGCTCAACCATTACATAACCCCATGTCTTCCTCTTAGAGGCACAATTACTGCTTCTGGTGACCTCGTCCCTCTCTCTTACATTGTTGCACTTTTGATTGGTAGGCCCAATTCTAGGGCCATTGGACCGAGTGACAAATGTCTCAACCCAAACCAGGCCTTCCATCTCGCAGAGATCAATGATGGATTCTTCCATTTGCAGCCGAAAAGAAGGTCTTGCTCTTGTTAA

Coding sequence (CDS)

ATGAAATGTTGGCATGGGGTTGCGGCTCGACCTCAAGTGGAGGCTAGTAGTAAATGGGTTGTCGATAGTCTGAATAAAGGGACCGATAGTTATGGCATAACGACTAGTTTTGGAGCCACATCCCATCGAAGGACCGATCGAGGAGAAACCCTTCAAAAAGAGCTTATTAGATTTTTAAACGCTGGAATTTTTGGGAATGATCCAAATTCTCTCAACACATTGCCACAGTCAGCAACACGAGCCGCTATGCTTGTGAGAATCAACACTCTTTTGCAAGGCTATTCGAGGATTAGGTTTGAAATTTTAGAAGCCATAACCAGATTGCTCAACCATTACATAACCCCATGTCTTCCTCTTAGAGGCACAATTACTGCTTCTGGTGACCTCGTCCCTCTCTCTTACATTGTTGCACTTTTGATTGGTAGGCCCAATTCTAGGGCCATTGGACCGAGTGACAAATGTCTCAACCCAAACCAGGCCTTCCATCTCGCAGAGATCAATGATGGATTCTTCCATTTGCAGCCGAAAAGAAGGTCTTGCTCTTGTTAA

Protein sequence

MKCWHGVAARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGNDPNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASGDLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPKRRSCSC
Homology
BLAST of Moc04g33840 vs. NCBI nr
Match: XP_038883983.1 (phenylalanine ammonia-lyase G4-like [Benincasa hispida])

HSP 1 Score: 276.2 bits (705), Expect = 2.1e-70
Identity = 136/168 (80.95%), Postives = 148/168 (88.10%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGNDP 68
           ARP VEASS+WV  S+  GTDSYG+TT FG+TSHRRTD+GETLQKELIRFLNAGIFGND 
Sbjct: 83  ARPAVEASSRWVFKSITNGTDSYGVTTGFGSTSHRRTDQGETLQKELIRFLNAGIFGNDL 142

Query: 69  NSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASGD 128
           NS +TLPQ ATRAAM+VRINTLLQGYS IRFEILEAI +LLNH ITPCLPLRGTITASGD
Sbjct: 143 NSCHTLPQQATRAAMVVRINTLLQGYSGIRFEILEAIAKLLNHNITPCLPLRGTITASGD 202

Query: 129 LVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           LVPLSYI  LLIGRPNS+AIGP+ + LNP +AFHLAEI  GFF LQPK
Sbjct: 203 LVPLSYIAGLLIGRPNSKAIGPNGESLNPIEAFHLAEIGGGFFSLQPK 250

BLAST of Moc04g33840 vs. NCBI nr
Match: XP_004149916.1 (phenylalanine ammonia-lyase G4 [Cucumis sativus] >KGN47834.1 hypothetical protein Csa_002790 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 1.6e-67
Identity = 131/168 (77.98%), Postives = 145/168 (86.31%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGNDP 68
           AR  VEASS+WVV+S+  GTDSYG+TT FG+TSHRRTD+GE LQKELIRFLNAGIFG D 
Sbjct: 83  ARSAVEASSRWVVESMTNGTDSYGVTTGFGSTSHRRTDQGEALQKELIRFLNAGIFGKDS 142

Query: 69  NSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASGD 128
           NS +TL Q ATRAAM+VRINTLLQGYS IRFEILEAI +LLNH ITPCLPLRGTITASGD
Sbjct: 143 NSCHTLSQQATRAAMVVRINTLLQGYSGIRFEILEAIAKLLNHNITPCLPLRGTITASGD 202

Query: 129 LVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           LVPLSYI  LL GRPNS+AIGP+ + LN N+AFHLA I  GFF+LQPK
Sbjct: 203 LVPLSYIAGLLTGRPNSKAIGPNGESLNSNEAFHLAGITSGFFNLQPK 250

BLAST of Moc04g33840 vs. NCBI nr
Match: AYK27181.1 (phenylalanine ammonia lyase [Juglans sigillata])

HSP 1 Score: 263.1 bits (671), Expect = 1.8e-66
Identity = 130/169 (76.92%), Postives = 146/169 (86.39%), Query Frame = 0

Query: 8   AARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGND 67
           AAR  V+ASS WV+DS+NKGTDSYG+TT FGATSHRRT +G  LQKELIRFLNAGIFGND
Sbjct: 86  AARAGVKASSDWVMDSMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGND 145

Query: 68  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 127
             S +TLP SATRAAMLVRINTLLQGYS IRFEI+EAIT+LLNH ITPCLPLRGTI+ASG
Sbjct: 146 TESCHTLPHSATRAAMLVRINTLLQGYSGIRFEIMEAITKLLNHNITPCLPLRGTISASG 205

Query: 128 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSYI  LLIGRPNS+A+GP+ + L+  +AF +A IN GFF LQPK
Sbjct: 206 DLVPLSYIAGLLIGRPNSKAVGPNGESLDATKAFQVAGINGGFFELQPK 254

BLAST of Moc04g33840 vs. NCBI nr
Match: XP_035539291.1 (phenylalanine ammonia-lyase G4 [Juglans regia] >KAF5453424.1 hypothetical protein F2P56_028327 [Juglans regia])

HSP 1 Score: 263.1 bits (671), Expect = 1.8e-66
Identity = 130/169 (76.92%), Postives = 146/169 (86.39%), Query Frame = 0

Query: 8   AARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGND 67
           AAR  V+ASS WV+DS+NKGTDSYG+TT FGATSHRRT +G  LQKELIRFLNAGIFGND
Sbjct: 88  AARAGVKASSDWVMDSMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGND 147

Query: 68  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 127
             S +TLP SATRAAMLVRINTLLQGYS IRFEI+EAIT+LLNH ITPCLPLRGTI+ASG
Sbjct: 148 TESCHTLPHSATRAAMLVRINTLLQGYSGIRFEIMEAITKLLNHNITPCLPLRGTISASG 207

Query: 128 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSYI  LLIGRPNS+A+GP+ + L+  +AF +A IN GFF LQPK
Sbjct: 208 DLVPLSYIAGLLIGRPNSKAVGPNGESLDATKAFQVAGINGGFFELQPK 256

BLAST of Moc04g33840 vs. NCBI nr
Match: XP_040999826.1 (phenylalanine ammonia-lyase G4 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 263.1 bits (671), Expect = 1.8e-66
Identity = 130/169 (76.92%), Postives = 146/169 (86.39%), Query Frame = 0

Query: 8   AARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGND 67
           AAR  V+ASS WV+DS+NKGTDSYG+TT FGATSHRRT +G  LQKELIRFLNAGIFGND
Sbjct: 86  AARAGVKASSDWVMDSMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGND 145

Query: 68  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 127
             S +TLP SATRAAMLVRINTLLQGYS IRFEI+EAIT+LLNH ITPCLPLRGTI+ASG
Sbjct: 146 TESCHTLPHSATRAAMLVRINTLLQGYSGIRFEIMEAITKLLNHNITPCLPLRGTISASG 205

Query: 128 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSYI  LLIGRPNS+A+GP+ + L+  +AF +A IN GFF LQPK
Sbjct: 206 DLVPLSYIAGLLIGRPNSKAVGPNGESLDATKAFQVAGINGGFFELQPK 254

BLAST of Moc04g33840 vs. ExPASy Swiss-Prot
Match: P45733 (Phenylalanine ammonia-lyase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 3.4e-68
Identity = 127/168 (75.60%), Postives = 142/168 (84.52%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGNDP 68
           AR  V+ASS WV+DS++KGTDSYG+TT FGATSHRRT  G  LQKELIRFLNAG+FGN  
Sbjct: 82  ARAGVKASSDWVMDSMSKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGVFGNGT 141

Query: 69  NSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASGD 128
            S +TLPQS TRAAMLVRINTLLQGYS IRFEILEAIT+LLNH +TPCLPLRGTITASGD
Sbjct: 142 ESCHTLPQSGTRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNVTPCLPLRGTITASGD 201

Query: 129 LVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           LVPLSYI  LL GRPNS+AIGP+ + LN  +AF +A +N GFF LQPK
Sbjct: 202 LVPLSYIAGLLTGRPNSKAIGPNGETLNAEEAFRVAGVNSGFFELQPK 249

BLAST of Moc04g33840 vs. ExPASy Swiss-Prot
Match: P35513 (Phenylalanine ammonia-lyase OS=Nicotiana tabacum OX=4097 GN=PALA PE=2 SV=2)

HSP 1 Score: 258.1 bits (658), Expect = 7.6e-68
Identity = 126/168 (75.00%), Postives = 141/168 (83.93%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGNDP 68
           AR  V+ASS WV+DS+ KGTDSYG+TT FGATSHRRT  G  LQKELIRFLNAG+FGN  
Sbjct: 82  ARAGVKASSDWVMDSMGKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGVFGNGT 141

Query: 69  NSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASGD 128
            S +TLPQS TRAAMLVRINTLLQGYS IRFEILEAIT+LLNH +TPCLPLRGTITASGD
Sbjct: 142 ESCHTLPQSGTRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNVTPCLPLRGTITASGD 201

Query: 129 LVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           LVPLSYI  LL GRPNS+A+GP+ + LN  +AF +A +N GFF LQPK
Sbjct: 202 LVPLSYIAGLLTGRPNSKAVGPNGETLNAEEAFRVAGVNGGFFELQPK 249

BLAST of Moc04g33840 vs. ExPASy Swiss-Prot
Match: P25872 (Phenylalanine ammonia-lyase OS=Nicotiana tabacum OX=4097 GN=TPA1 PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 8.4e-67
Identity = 125/168 (74.40%), Postives = 141/168 (83.93%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGNDP 68
           AR  V+ASS WV+DS+NKGTDSYG+TT FGATSHRRT  G  LQKELIRFLNAG+FGN  
Sbjct: 85  ARAGVKASSDWVMDSMNKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGVFGNGT 144

Query: 69  NSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASGD 128
            + +TLP SATRAAMLVRINTLLQGYS IRFEILEAIT+L+N  ITPCLPLRGTITASGD
Sbjct: 145 ETSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLINSNITPCLPLRGTITASGD 204

Query: 129 LVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           LVPLSYI  LL GRPNS+A+GP+ + LN  +AF +A +N GFF LQPK
Sbjct: 205 LVPLSYIAGLLTGRPNSKAVGPNGETLNAEEAFRVAGVNGGFFELQPK 252

BLAST of Moc04g33840 vs. ExPASy Swiss-Prot
Match: Q42667 (Phenylalanine ammonia-lyase OS=Citrus limon OX=2708 GN=PAL6 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 1.1e-66
Identity = 125/169 (73.96%), Postives = 141/169 (83.43%), Query Frame = 0

Query: 8   AARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGND 67
           AAR  V+ASS WV+DS+ KGTDSYG+TT FGATSHRRT +G  LQKELIRFLN+GIFGN 
Sbjct: 89  AARAGVKASSDWVMDSMMKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNSGIFGNG 148

Query: 68  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 127
             S +TLP SATRAAMLVR+NTLLQGYS IRFEILE IT+ LNH ITPCLPLRGTITASG
Sbjct: 149 TESSHTLPHSATRAAMLVRVNTLLQGYSGIRFEILETITKFLNHNITPCLPLRGTITASG 208

Query: 128 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSYI  LL GRPNS+A+G + + LNP +AF+LA +  GFF LQPK
Sbjct: 209 DLVPLSYIAGLLTGRPNSKAVGSNGQVLNPTEAFNLAGVTSGFFELQPK 257

BLAST of Moc04g33840 vs. ExPASy Swiss-Prot
Match: O49835 (Phenylalanine ammonia-lyase 1 OS=Lithospermum erythrorhizon OX=34254 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.4e-66
Identity = 127/169 (75.15%), Postives = 142/169 (84.02%), Query Frame = 0

Query: 8   AARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGND 67
           AAR  V+ASS WV+DS+NKGTDSYG+TT FGATSHRRT +G  LQKELIRFLNAGIFGN 
Sbjct: 79  AAREGVKASSDWVMDSMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNG 138

Query: 68  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 127
             + +TLP SATRAAMLVRINTLLQGYS IRFEILEAIT+ LN  ITPCLPLRGTITASG
Sbjct: 139 TETSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKFLNTNITPCLPLRGTITASG 198

Query: 128 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSYI  LL GRPNS+A+GP+ + +N  +AF LA I+ GFF LQPK
Sbjct: 199 DLVPLSYIAGLLTGRPNSKAVGPTGEKINAEEAFRLAGISTGFFELQPK 247

BLAST of Moc04g33840 vs. ExPASy TrEMBL
Match: A0A0A0KG52 (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_6G405960 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 7.9e-68
Identity = 131/168 (77.98%), Postives = 145/168 (86.31%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGNDP 68
           AR  VEASS+WVV+S+  GTDSYG+TT FG+TSHRRTD+GE LQKELIRFLNAGIFG D 
Sbjct: 83  ARSAVEASSRWVVESMTNGTDSYGVTTGFGSTSHRRTDQGEALQKELIRFLNAGIFGKDS 142

Query: 69  NSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASGD 128
           NS +TL Q ATRAAM+VRINTLLQGYS IRFEILEAI +LLNH ITPCLPLRGTITASGD
Sbjct: 143 NSCHTLSQQATRAAMVVRINTLLQGYSGIRFEILEAIAKLLNHNITPCLPLRGTITASGD 202

Query: 129 LVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           LVPLSYI  LL GRPNS+AIGP+ + LN N+AFHLA I  GFF+LQPK
Sbjct: 203 LVPLSYIAGLLTGRPNSKAIGPNGESLNSNEAFHLAGITSGFFNLQPK 250

BLAST of Moc04g33840 vs. ExPASy TrEMBL
Match: A0A0K8WMM3 (Phenylalanine ammonia-lyase OS=Acacia koa OX=468172 GN=pal1 PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.4e-67
Identity = 130/169 (76.92%), Postives = 145/169 (85.80%), Query Frame = 0

Query: 8   AARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGND 67
           +AR  V+ASS WV+DS+NKGTDSYG+TT FGATSHRRT +G  LQKELIRFLNAGIFGN 
Sbjct: 84  SARAGVKASSDWVMDSMNKGTDSYGVTTGFGATSHRRTKQGAALQKELIRFLNAGIFGNG 143

Query: 68  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 127
             S +TLP SATRAAMLVRINTLLQGYS IRFEILEA+T+ LNH ITPCLPLRGTITASG
Sbjct: 144 TESCHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAMTKFLNHNITPCLPLRGTITASG 203

Query: 128 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSY+  LLIGRPNS++IGP  + L+P +AFHLA IN GFF LQPK
Sbjct: 204 DLVPLSYVAGLLIGRPNSKSIGPDGRVLSPKEAFHLAGINGGFFELQPK 252

BLAST of Moc04g33840 vs. ExPASy TrEMBL
Match: A0A3G1VTD0 (Phenylalanine ammonia-lyase OS=Juglans sigillata OX=224355 GN=PAL PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 8.8e-67
Identity = 130/169 (76.92%), Postives = 146/169 (86.39%), Query Frame = 0

Query: 8   AARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGND 67
           AAR  V+ASS WV+DS+NKGTDSYG+TT FGATSHRRT +G  LQKELIRFLNAGIFGND
Sbjct: 86  AARAGVKASSDWVMDSMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGND 145

Query: 68  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 127
             S +TLP SATRAAMLVRINTLLQGYS IRFEI+EAIT+LLNH ITPCLPLRGTI+ASG
Sbjct: 146 TESCHTLPHSATRAAMLVRINTLLQGYSGIRFEIMEAITKLLNHNITPCLPLRGTISASG 205

Query: 128 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSYI  LLIGRPNS+A+GP+ + L+  +AF +A IN GFF LQPK
Sbjct: 206 DLVPLSYIAGLLIGRPNSKAVGPNGESLDATKAFQVAGINGGFFELQPK 254

BLAST of Moc04g33840 vs. ExPASy TrEMBL
Match: A0A6P9DVU6 (Phenylalanine ammonia-lyase OS=Juglans regia OX=51240 GN=LOC109008959 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 8.8e-67
Identity = 130/169 (76.92%), Postives = 146/169 (86.39%), Query Frame = 0

Query: 8   AARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGND 67
           AAR  V+ASS WV+DS+NKGTDSYG+TT FGATSHRRT +G  LQKELIRFLNAGIFGND
Sbjct: 88  AARAGVKASSDWVMDSMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGND 147

Query: 68  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 127
             S +TLP SATRAAMLVRINTLLQGYS IRFEI+EAIT+LLNH ITPCLPLRGTI+ASG
Sbjct: 148 TESCHTLPHSATRAAMLVRINTLLQGYSGIRFEIMEAITKLLNHNITPCLPLRGTISASG 207

Query: 128 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSYI  LLIGRPNS+A+GP+ + L+  +AF +A IN GFF LQPK
Sbjct: 208 DLVPLSYIAGLLIGRPNSKAVGPNGESLDATKAFQVAGINGGFFELQPK 256

BLAST of Moc04g33840 vs. ExPASy TrEMBL
Match: A0A200R5P5 (Phenylalanine ammonia-lyase OS=Macleaya cordata OX=56857 GN=BVC80_1835g459 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.5e-66
Identity = 130/169 (76.92%), Postives = 145/169 (85.80%), Query Frame = 0

Query: 8   AARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGND 67
           +AR  V+ASS WV++S+NKGTDSYG+TT FGATSHRRT +G  LQKELIRFLNAGIFGN 
Sbjct: 83  SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNG 142

Query: 68  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 127
             S +TLP SATRAAMLVRINTLLQGYS IRFEILEAIT+ LNH ITPCLPLRGTITASG
Sbjct: 143 TESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKFLNHNITPCLPLRGTITASG 202

Query: 128 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSYI  LL GRPNS+A+GP+ + L+P QAF+LA IN GFF LQPK
Sbjct: 203 DLVPLSYIAGLLTGRPNSKAVGPNGESLDPTQAFNLAGINGGFFELQPK 251

BLAST of Moc04g33840 vs. TAIR 10
Match: AT3G53260.1 (phenylalanine ammonia-lyase 2 )

HSP 1 Score: 242.7 bits (618), Expect = 2.4e-64
Identity = 122/168 (72.62%), Postives = 136/168 (80.95%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGNDP 68
           +R  V+ASS WV++S+NKGTDSYG+TT FGATSHRRT  G  LQ ELIRFLNAGIFGN  
Sbjct: 87  SRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLNAGIFGNTK 146

Query: 69  NSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASGD 128
            + +TLPQSATRAAMLVR+NTLLQGYS IRFEILEAIT LLNH I+P LPLRGTITASGD
Sbjct: 147 ETCHTLPQSATRAAMLVRVNTLLQGYSGIRFEILEAITSLLNHNISPSLPLRGTITASGD 206

Query: 129 LVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           LVPLSYI  LL GRPNS+A GP  + L   +AF  A I+ GFF LQPK
Sbjct: 207 LVPLSYIAGLLTGRPNSKATGPDGESLTAKEAFEKAGISTGFFDLQPK 254

BLAST of Moc04g33840 vs. TAIR 10
Match: AT2G37040.1 (PHE ammonia lyase 1 )

HSP 1 Score: 240.4 bits (612), Expect = 1.2e-63
Identity = 122/168 (72.62%), Postives = 136/168 (80.95%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFGNDP 68
           AR  V ASS WV++S+NKGTDSYG+TT FGATSHRRT  G  LQKELIRFLNAGIFG+  
Sbjct: 95  ARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFLNAGIFGSTK 154

Query: 69  NSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASGD 128
            + +TLP SATRAAMLVRINTLLQG+S IRFEILEAIT  LN+ ITP LPLRGTITASGD
Sbjct: 155 ETSHTLPHSATRAAMLVRINTLLQGFSGIRFEILEAITSFLNNNITPSLPLRGTITASGD 214

Query: 129 LVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           LVPLSYI  LL GRPNS+A GP+ + L   +AF LA I+ GFF LQPK
Sbjct: 215 LVPLSYIAGLLTGRPNSKATGPNGEALTAEEAFKLAGISSGFFDLQPK 262

BLAST of Moc04g33840 vs. TAIR 10
Match: AT3G10340.1 (phenylalanine ammonia-lyase 4 )

HSP 1 Score: 236.5 bits (602), Expect = 1.7e-62
Identity = 120/169 (71.01%), Postives = 141/169 (83.43%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIFG-ND 68
           AR  V+ASS+WV++S+N+GTDSYG+TT FGATSHRRT +G  LQ ELIRFLNAGIFG   
Sbjct: 77  ARAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQNELIRFLNAGIFGPGA 136

Query: 69  PNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITASG 128
            ++ +TLP+  TRAAMLVR+NTLLQGYS IRFEILEAIT+LLNH ITPCLPLRGTITASG
Sbjct: 137 GDTSHTLPKPTTRAAMLVRVNTLLQGYSGIRFEILEAITKLLNHEITPCLPLRGTITASG 196

Query: 129 DLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           DLVPLSYI  LL GRPNS+A+GPS + L  ++AF LA ++  FF LQPK
Sbjct: 197 DLVPLSYIAGLLTGRPNSKAVGPSGETLTASEAFKLAGVS-SFFELQPK 244

BLAST of Moc04g33840 vs. TAIR 10
Match: AT5G04230.1 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 221.1 bits (562), Expect = 7.3e-58
Identity = 117/171 (68.42%), Postives = 138/171 (80.70%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIF--GN 68
           AR  V+ASS WV++S+N+ TD+YGITT FG++S RRTD+G  LQKELIR+LNAGIF  GN
Sbjct: 72  ARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFATGN 131

Query: 69  -DPNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITA 128
            D +  NTLP+ ATRAAML+R+NTLLQGYS IRFEILEAIT LLN  ITP LPLRGTITA
Sbjct: 132 EDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTITA 191

Query: 129 SGDLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           SGDLVPLSYI   LIGRPNSR++GPS + L   +AF LA ++  FF L+PK
Sbjct: 192 SGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGVS-SFFELRPK 241

BLAST of Moc04g33840 vs. TAIR 10
Match: AT5G04230.2 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 221.1 bits (562), Expect = 7.3e-58
Identity = 117/171 (68.42%), Postives = 138/171 (80.70%), Query Frame = 0

Query: 9   ARPQVEASSKWVVDSLNKGTDSYGITTSFGATSHRRTDRGETLQKELIRFLNAGIF--GN 68
           AR  V+ASS WV++S+N+ TD+YGITT FG++S RRTD+G  LQKELIR+LNAGIF  GN
Sbjct: 72  ARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFATGN 131

Query: 69  -DPNSLNTLPQSATRAAMLVRINTLLQGYSRIRFEILEAITRLLNHYITPCLPLRGTITA 128
            D +  NTLP+ ATRAAML+R+NTLLQGYS IRFEILEAIT LLN  ITP LPLRGTITA
Sbjct: 132 EDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTITA 191

Query: 129 SGDLVPLSYIVALLIGRPNSRAIGPSDKCLNPNQAFHLAEINDGFFHLQPK 177
           SGDLVPLSYI   LIGRPNSR++GPS + L   +AF LA ++  FF L+PK
Sbjct: 192 SGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGVS-SFFELRPK 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883983.12.1e-7080.95phenylalanine ammonia-lyase G4-like [Benincasa hispida][more]
XP_004149916.11.6e-6777.98phenylalanine ammonia-lyase G4 [Cucumis sativus] >KGN47834.1 hypothetical protei... [more]
AYK27181.11.8e-6676.92phenylalanine ammonia lyase [Juglans sigillata][more]
XP_035539291.11.8e-6676.92phenylalanine ammonia-lyase G4 [Juglans regia] >KAF5453424.1 hypothetical protei... [more]
XP_040999826.11.8e-6676.92phenylalanine ammonia-lyase G4 [Juglans microcarpa x Juglans regia][more]
Match NameE-valueIdentityDescription
P457333.4e-6875.60Phenylalanine ammonia-lyase OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P355137.6e-6875.00Phenylalanine ammonia-lyase OS=Nicotiana tabacum OX=4097 GN=PALA PE=2 SV=2[more]
P258728.4e-6774.40Phenylalanine ammonia-lyase OS=Nicotiana tabacum OX=4097 GN=TPA1 PE=2 SV=1[more]
Q426671.1e-6673.96Phenylalanine ammonia-lyase OS=Citrus limon OX=2708 GN=PAL6 PE=2 SV=1[more]
O498351.4e-6675.15Phenylalanine ammonia-lyase 1 OS=Lithospermum erythrorhizon OX=34254 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KG527.9e-6877.98Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_6G405960 PE=3 SV=1[more]
A0A0K8WMM31.4e-6776.92Phenylalanine ammonia-lyase OS=Acacia koa OX=468172 GN=pal1 PE=3 SV=1[more]
A0A3G1VTD08.8e-6776.92Phenylalanine ammonia-lyase OS=Juglans sigillata OX=224355 GN=PAL PE=2 SV=1[more]
A0A6P9DVU68.8e-6776.92Phenylalanine ammonia-lyase OS=Juglans regia OX=51240 GN=LOC109008959 PE=3 SV=1[more]
A0A200R5P51.5e-6676.92Phenylalanine ammonia-lyase OS=Macleaya cordata OX=56857 GN=BVC80_1835g459 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT3G53260.12.4e-6472.62phenylalanine ammonia-lyase 2 [more]
AT2G37040.11.2e-6372.62PHE ammonia lyase 1 [more]
AT3G10340.11.7e-6271.01phenylalanine ammonia-lyase 4 [more]
AT5G04230.17.3e-5868.42phenyl alanine ammonia-lyase 3 [more]
AT5G04230.27.3e-5868.42phenyl alanine ammonia-lyase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001106Aromatic amino acid lyasePFAMPF00221Lyase_aromaticcoord: 8..160
e-value: 3.1E-41
score: 141.5
IPR001106Aromatic amino acid lyasePANTHERPTHR10362HISTIDINE AMMONIA-LYASEcoord: 8..177
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 5..178
e-value: 1.6E-58
score: 200.0
NoneNo IPR availablePANTHERPTHR10362:SF51PHENYLALANINE AMMONIA-LYASEcoord: 8..177
IPR022313Phenylalanine/histidine ammonia-lyases, active sitePROSITEPS00488PAL_HISTIDASEcoord: 121..137
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 7..176

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g33840.1Moc04g33840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009800 cinnamic acid biosynthetic process
biological_process GO:0006559 L-phenylalanine catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0045548 phenylalanine ammonia-lyase activity
molecular_function GO:0016841 ammonia-lyase activity
molecular_function GO:0003824 catalytic activity