Moc04g29880 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g29880
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptiontransmembrane protein 53
Locationchr4: 22254348 .. 22256590 (-)
RNA-Seq ExpressionMoc04g29880
SyntenyMoc04g29880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCCTTGTCCGGAATCCTTCAACGACCATTCTTTGCTGTAACTGCGGTGGCTGTCGCTTCGTTTTCCTCTGACTTTTCAGACAAGTTACCATCTCAGAGATCGCCAGATGCTGCTGCTTCTTCTTCTGCTTCCCCAGATTTGTCCAACAATTCAACTTTTGATTTCTTGAAGGAATCAAAGACTTTATTTGCTTCCCATATTTCTGTTTCAAAGCTTGCAAACTTATCATTTGTCACCAAAATTCATGCACCAGTTCCCAACATTAATTTTCCGATTCCAACATCCAGTGGTAATTATGTGCGAAACTTTCACACATCTCTGGTTTCTTCCCCCGTTCTTTCCAATTTATATCAGTCAGCTGCATTGACCAAGGCCTCAAAGCAAGCTGCATCTTCACATAAAATTTTTTCCCCACCTTCTGAGGTTATGTATAGATGGCATTTACCTGAGCCAACATCTTTAGATATTTCGGGTAGTTCTGCTTGTTCAGTGGCAAAATCTAGGACGGTGGTGGTTTTATTAGGATGGTTGGGAGCAAAGCAAAAACATTTGAAGAGATACGCTGAATGGTACACTTCAAGGGGTTTTCATGCGATTACCTTTACTTTTCCAATGTCTGAGATACTCACGTACCAGATTGGTGGGAAGGTTGAACAACACGTAGACTTGCTTGTGAATCATCTAGCTGATTGGTTGGAAGAGGAGCACGGGAAGAACCTTGTGTTCCACACTTTTAGTAATACCGGATGGTTGACGTACGTTTTTCCTCTATCCCCCTCTTCATTTAACATCCACACCACGCAGAATGAAAGTTTGGTACTTTTGACTTCACAGTTAGCACATTTGACTTCTGTTCAACAACCGTCTGTATGTGATTGTTCCCTTGGATTCATTTTGTTGCAGGTATGGAGCTATCCTGGAGAAGTTTCAGAAGCATGATTCTTCTGTAATGGGAAGGATTAAAGGCTGCATTGTGGATTCTGCCCCTGTTGCAGCTCCTGACCCCCAGGTAATGATGCCCTTGATATATAAATTTGTGTTCATCCTTAACGAGACATTCGTCGCAATAGATACATAGTTACCACTTACTAGTGAACCATTTTGGCACCAGTTCTTTTTCTTGTCATTCAAAAATTGATCGATTGCTGTATATATGCACCATAATAAGAAACCTTGTAGTATGAACTATGCTTTGATTTAAAGAAAAAAGAAAATCATTGGAAAGGTAGAAATTTATCGTAGTCGACCTGTTATGGTATTATATAGTAAAAGGCTCAGAGTAACCTTTTTGTCCTTGCACATCCTCCATATAACAAGTTATGAGAAAAGGGTGAATTTTTGTTTGTTATATAACTCATTATCTTCTCTATTAATTTAGAGTATCTGTTTGCTGGGCGTTTAGGTTATGACAGCTTCTTTTATTTTAATTGCACTTTCCATTCAAGGAGTTTCCCTTTCAGTTATGGATTGTAGATGGTCTGTGTTTCTACTTGAGTTTCTTGTTCGAACAATTATGAGGGAAATGTTCATTAAATCTTTGATATTGGTTTTCTAGGTCTGGGCTTCAGGCTTCTCTGCAGCCTTTTTGAAAAAGAACAGTGTAGCAACAAAAGGATCGACGATGTTGGACAACACAAGGATGGAAGTATCGATCCGCAATAAAGACGATAAAGAAAGTAAACCAGCAGTTACTGAAGCAGCACTTCTAATGGTTTTGGAAAAATTCTTCGGCGTCGTTTTGAACCTTCCTACTGTGAACAGGTAGTTAAGTCGGCCTTTCAAATGTGATCATTCTAATCAATACTAGATCAAAGATGATGCAAAAATATACAGGATCAAGTAGTCAGTTCAAAAACATGATAGTTTCATATCCTTCAAATGTGCCTGTTTTTCGATCATGTATAGATTTCGATAATTTATCCATGAAATCTAAAAGACGAAAATTGTTCTCTTTTCCTACCAGGAGGCTATCTGATGTGTTGGACATGTTATCGTCGTCGCAACCGAGCTGCCCTCAACTGTACATCTACAGCTCTGCTGATCGAGTCATCCCGGCCAATTCTGTGGAATCGTTTATAGAGGAGCAACGGCGAGCGGGACGGGAGGTTAGAGCTTGCAACTTTGTGTCCACCCCTCATGTAGATCATTATAGGAATGACCCAAAACTGTATAGTTCTCAGCTTAGTCAGTTTCTGGCAGATTGCGTGCTTACTTCTTGTTGCAGAGAGTCTTGTTAA

mRNA sequence

ATGGGTTCCTTGTCCGGAATCCTTCAACGACCATTCTTTGCTGTAACTGCGGTGGCTGTCGCTTCGTTTTCCTCTGACTTTTCAGACAAGTTACCATCTCAGAGATCGCCAGATGCTGCTGCTTCTTCTTCTGCTTCCCCAGATTTGTCCAACAATTCAACTTTTGATTTCTTGAAGGAATCAAAGACTTTATTTGCTTCCCATATTTCTGTTTCAAAGCTTGCAAACTTATCATTTGTCACCAAAATTCATGCACCAGTTCCCAACATTAATTTTCCGATTCCAACATCCAGTGGTAATTATGTGCGAAACTTTCACACATCTCTGGTTTCTTCCCCCGTTCTTTCCAATTTATATCAGTCAGCTGCATTGACCAAGGCCTCAAAGCAAGCTGCATCTTCACATAAAATTTTTTCCCCACCTTCTGAGGTTATGTATAGATGGCATTTACCTGAGCCAACATCTTTAGATATTTCGGGTAGTTCTGCTTGTTCAGTGGCAAAATCTAGGACGGTGGTGGTTTTATTAGGATGGTTGGGAGCAAAGCAAAAACATTTGAAGAGATACGCTGAATGGTACACTTCAAGGGGTTTTCATGCGATTACCTTTACTTTTCCAATGTCTGAGATACTCACGTACCAGATTGGTGGGAAGGTTGAACAACACGTAGACTTGCTTGTGAATCATCTAGCTGATTGGTTGGAAGAGGAGCACGGGAAGAACCTTGTGTTCCACACTTTTAGTAATACCGGATGGTTGACGTATGGAGCTATCCTGGAGAAGTTTCAGAAGCATGATTCTTCTGTAATGGGAAGGATTAAAGGCTGCATTGTGGATTCTGCCCCTGTTGCAGCTCCTGACCCCCAGGTCTGGGCTTCAGGCTTCTCTGCAGCCTTTTTGAAAAAGAACAGTGTAGCAACAAAAGGATCGACGATGTTGGACAACACAAGGATGGAAGTATCGATCCGCAATAAAGACGATAAAGAAAGTAAACCAGCAGTTACTGAAGCAGCACTTCTAATGGTTTTGGAAAAATTCTTCGGCGTCGTTTTGAACCTTCCTACTGTGAACAGGAGGCTATCTGATGTGTTGGACATGTTATCGTCGTCGCAACCGAGCTGCCCTCAACTGTACATCTACAGCTCTGCTGATCGAGTCATCCCGGCCAATTCTGTGGAATCGTTTATAGAGGAGCAACGGCGAGCGGGACGGGAGGTTAGAGCTTGCAACTTTGTGTCCACCCCTCATGTAGATCATTATAGGAATGACCCAAAACTGTATAGTTCTCAGCTTAGTCAGTTTCTGGCAGATTGCGTGCTTACTTCTTGTTGCAGAGAGTCTTGTTAA

Coding sequence (CDS)

ATGGGTTCCTTGTCCGGAATCCTTCAACGACCATTCTTTGCTGTAACTGCGGTGGCTGTCGCTTCGTTTTCCTCTGACTTTTCAGACAAGTTACCATCTCAGAGATCGCCAGATGCTGCTGCTTCTTCTTCTGCTTCCCCAGATTTGTCCAACAATTCAACTTTTGATTTCTTGAAGGAATCAAAGACTTTATTTGCTTCCCATATTTCTGTTTCAAAGCTTGCAAACTTATCATTTGTCACCAAAATTCATGCACCAGTTCCCAACATTAATTTTCCGATTCCAACATCCAGTGGTAATTATGTGCGAAACTTTCACACATCTCTGGTTTCTTCCCCCGTTCTTTCCAATTTATATCAGTCAGCTGCATTGACCAAGGCCTCAAAGCAAGCTGCATCTTCACATAAAATTTTTTCCCCACCTTCTGAGGTTATGTATAGATGGCATTTACCTGAGCCAACATCTTTAGATATTTCGGGTAGTTCTGCTTGTTCAGTGGCAAAATCTAGGACGGTGGTGGTTTTATTAGGATGGTTGGGAGCAAAGCAAAAACATTTGAAGAGATACGCTGAATGGTACACTTCAAGGGGTTTTCATGCGATTACCTTTACTTTTCCAATGTCTGAGATACTCACGTACCAGATTGGTGGGAAGGTTGAACAACACGTAGACTTGCTTGTGAATCATCTAGCTGATTGGTTGGAAGAGGAGCACGGGAAGAACCTTGTGTTCCACACTTTTAGTAATACCGGATGGTTGACGTATGGAGCTATCCTGGAGAAGTTTCAGAAGCATGATTCTTCTGTAATGGGAAGGATTAAAGGCTGCATTGTGGATTCTGCCCCTGTTGCAGCTCCTGACCCCCAGGTCTGGGCTTCAGGCTTCTCTGCAGCCTTTTTGAAAAAGAACAGTGTAGCAACAAAAGGATCGACGATGTTGGACAACACAAGGATGGAAGTATCGATCCGCAATAAAGACGATAAAGAAAGTAAACCAGCAGTTACTGAAGCAGCACTTCTAATGGTTTTGGAAAAATTCTTCGGCGTCGTTTTGAACCTTCCTACTGTGAACAGGAGGCTATCTGATGTGTTGGACATGTTATCGTCGTCGCAACCGAGCTGCCCTCAACTGTACATCTACAGCTCTGCTGATCGAGTCATCCCGGCCAATTCTGTGGAATCGTTTATAGAGGAGCAACGGCGAGCGGGACGGGAGGTTAGAGCTTGCAACTTTGTGTCCACCCCTCATGTAGATCATTATAGGAATGACCCAAAACTGTATAGTTCTCAGCTTAGTCAGTTTCTGGCAGATTGCGTGCTTACTTCTTGTTGCAGAGAGTCTTGTTAA

Protein sequence

MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKESKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQSAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGKNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRLSDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHYRNDPKLYSSQLSQFLADCVLTSCCRESC
Homology
BLAST of Moc04g29880 vs. NCBI nr
Match: XP_022141695.1 (transmembrane protein 53 [Momordica charantia] >XP_022141696.1 transmembrane protein 53 [Momordica charantia])

HSP 1 Score: 880.9 bits (2275), Expect = 4.5e-252
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE
Sbjct: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ
Sbjct: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL
Sbjct: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY
Sbjct: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 449
           RNDPKLYSSQLSQFLADCVLTSCCRESC
Sbjct: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 448

BLAST of Moc04g29880 vs. NCBI nr
Match: XP_008465096.1 (PREDICTED: transmembrane protein 53 [Cucumis melo] >XP_008465105.1 PREDICTED: transmembrane protein 53 [Cucumis melo] >XP_016903289.1 PREDICTED: transmembrane protein 53 [Cucumis melo])

HSP 1 Score: 763.5 bits (1970), Expect = 1.0e-216
Identity = 388/448 (86.61%), Postives = 414/448 (92.41%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSLSGILQRPF A+TAVA+ASFSSDFSDKLPSQ+  + ++SSS+S   S++ST DFLKE
Sbjct: 1   MGSLSGILQRPFVALTAVAIASFSSDFSDKLPSQKPTEDSSSSSSS---SSDSTLDFLKE 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           S T F SH SVSKLANLSFVTKIHAP+P  +FPIPTSS NYV NF TSLVSS VLSNLYQ
Sbjct: 61  SNTSFVSHTSVSKLANLSFVTKIHAPIPKFSFPIPTSSCNYVGNFPTSLVSSSVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTK SKQ A S K+ SPPSEVMYRWHLPEPTSLDI+GSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKGSKQVAPSPKLSSPPSEVMYRWHLPEPTSLDITGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPM+EILTYQ+GGKVEQH+DLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQVGGKVEQHIDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQ HDSS+MGRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQNHDSSIMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKG T  DNT MEVSI +K++ E KPAVTEAALL++LEK FGVVLNLPTVNRRL
Sbjct: 301 KKNSVATKGLTTWDNTGMEVSISDKENIEPKPAVTEAALLLLLEKIFGVVLNLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVLD LSSSQPSCPQLYIYSSADRVIPA SVESFIEEQRRAGREVRACNFVSTPHVDH+
Sbjct: 361 SDVLDTLSSSQPSCPQLYIYSSADRVIPAGSVESFIEEQRRAGREVRACNFVSTPHVDHF 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 449
           RNDPKLY++QLSQFLADCVLT+CCRESC
Sbjct: 421 RNDPKLYTTQLSQFLADCVLTACCRESC 445

BLAST of Moc04g29880 vs. NCBI nr
Match: XP_004149868.2 (transmembrane protein 53 [Cucumis sativus] >XP_011655159.1 transmembrane protein 53 [Cucumis sativus] >KGN50943.1 hypothetical protein Csa_023082 [Cucumis sativus])

HSP 1 Score: 760.0 bits (1961), Expect = 1.2e-215
Identity = 388/448 (86.61%), Postives = 414/448 (92.41%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSLSGILQRPF A+TAVAVASFSSDFSDKLPSQ+  +A++SSS+S   S++ST DFLKE
Sbjct: 1   MGSLSGILQRPFVALTAVAVASFSSDFSDKLPSQKPTEASSSSSSSSSSSSDSTLDFLKE 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           S T F SH SVSKLANLSFVTKIHAP+P  +F  P+SS NYV NF TSLVSS VLSNLYQ
Sbjct: 61  SNTSFVSHTSVSKLANLSFVTKIHAPIPKFSF--PSSSCNYVGNFPTSLVSSSVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTK SKQAA S K+ SPPSEVMYRWHLPEPTSLDI+GSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKGSKQAAPSPKLSSPPSEVMYRWHLPEPTSLDITGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPM+EILTYQ+GGKVEQH+DLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLGGKVEQHIDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQ HDSS+MGRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQNHDSSIMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKG T  DNT MEVSI +K++ + KPAVTEAALL+VLEK FGVVLNLPTVNRRL
Sbjct: 301 KKNSVATKGLTTWDNTGMEVSISDKENIKPKPAVTEAALLLVLEKIFGVVLNLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVLD LSSSQPSCPQLYIYSSAD+VIPA SVESFIEEQRRAGREVRACNFVSTPHVDH+
Sbjct: 361 SDVLDTLSSSQPSCPQLYIYSSADQVIPAGSVESFIEEQRRAGREVRACNFVSTPHVDHF 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 449
           RNDPKLY++QLSQFLADCVLTSCCRESC
Sbjct: 421 RNDPKLYTTQLSQFLADCVLTSCCRESC 446

BLAST of Moc04g29880 vs. NCBI nr
Match: XP_038875313.1 (transmembrane protein 53 [Benincasa hispida] >XP_038875314.1 transmembrane protein 53 [Benincasa hispida])

HSP 1 Score: 753.4 bits (1944), Expect = 1.1e-213
Identity = 384/448 (85.71%), Postives = 412/448 (91.96%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSLSGILQRPF AVTA+AVASFSSDFSDKLPSQ+ P+A++SSS+S   S++S  +FLK 
Sbjct: 1   MGSLSGILQRPFVAVTAMAVASFSSDFSDKLPSQKPPEASSSSSSSS--SSDSALEFLKA 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           SKT F S+ SVS+LANLSFVTKIHAP+P  +FPIPTSS NYV N HTSLVSS VLSNLYQ
Sbjct: 61  SKTSFVSYTSVSELANLSFVTKIHAPIPKFSFPIPTSSCNYVGNIHTSLVSSSVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTKASKQAA S K+  PPSEVMYRWHLPEP+SL+I+GSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKASKQAAPSPKLSFPPSEVMYRWHLPEPSSLNITGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPM+EILTYQ+GGKVE+H+DLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLGGKVEEHIDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQ  DSS+MG IKGC+VDSAPVAAPDPQVWASGFSAA L
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQNRDSSLMGTIKGCVVDSAPVAAPDPQVWASGFSAALL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKGST LDNT MEVS  NK++ E KPAVTEAALL+VLEK FGVVL LPTVNRRL
Sbjct: 301 KKNSVATKGSTTLDNTGMEVSFSNKENIEPKPAVTEAALLLVLEKIFGVVLKLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVL  LSSSQPSCPQLYIYSSADRVIPA SVESFIEEQRRAGREVRACNFVSTPHVDH+
Sbjct: 361 SDVLGTLSSSQPSCPQLYIYSSADRVIPAGSVESFIEEQRRAGREVRACNFVSTPHVDHF 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 449
           RNDPKLY+SQLSQFLADCVLTSCCRESC
Sbjct: 421 RNDPKLYTSQLSQFLADCVLTSCCRESC 446

BLAST of Moc04g29880 vs. NCBI nr
Match: XP_023550651.1 (transmembrane protein 53-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 752.7 bits (1942), Expect = 1.9e-213
Identity = 384/448 (85.71%), Postives = 406/448 (90.62%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSL GILQRPF AVTAVAVASFSSDFSDKLPSQR P+A   S +SP  S+ S  DFLKE
Sbjct: 1   MGSLPGILQRPFVAVTAVAVASFSSDFSDKLPSQRPPEA---SFSSPSSSSESILDFLKE 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           SKT F SH SVSKLANLSFVTKIHAP+P  +FPIP SS NYV NF TSLVSS VLSNLYQ
Sbjct: 61  SKTSFVSHTSVSKLANLSFVTKIHAPIPKCSFPIPNSSCNYVGNFRTSLVSSSVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTKASKQAA S  +  PPSEVMYRWHLPEPTS+DI+GSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKASKQAAPSRNLSFPPSEVMYRWHLPEPTSVDITGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHL RYAEWYTSRGFHAITFTFPM+EIL YQ+GGKVEQH+DLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLNRYAEWYTSRGFHAITFTFPMAEILAYQLGGKVEQHIDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQ HDSS++GRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQNHDSSLLGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKG T LDNT ME+SI NK++ E KPAVTEAALL+VLEK FG VL LPTVNRRL
Sbjct: 301 KKNSVATKGLTTLDNTGMELSISNKENIEPKPAVTEAALLLVLEKIFGFVLKLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVL+ LS SQPSCPQLYIYSSADRVIPA+SVESFIEEQRRAG EVRACNFVSTPHVDH+
Sbjct: 361 SDVLNTLSLSQPSCPQLYIYSSADRVIPADSVESFIEEQRRAGHEVRACNFVSTPHVDHF 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 449
           RNDPKLY+SQLSQFLADC+LTSCCRESC
Sbjct: 421 RNDPKLYTSQLSQFLADCLLTSCCRESC 445

BLAST of Moc04g29880 vs. ExPASy Swiss-Prot
Match: Q9D0Z3 (Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 7.2e-19
Identity = 84/279 (30.11%), Postives = 119/279 (42.65%), Query Frame = 0

Query: 168 KSRTVVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHV--DL 227
           K + VV+LLGW G + K+L +Y+  Y  RG   I +T P   +   +  G     V    
Sbjct: 31  KQQPVVILLGWGGCRDKNLAKYSAIYHKRGCIVIRYTAPWHMVFFSESLGIPSLRVIAQK 90

Query: 228 LVNHLADWLEEEHGKNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAA 287
           L+  L D+  E   + L+FH FSN G + Y  +LE  Q H       + G I DS P   
Sbjct: 91  LLELLFDY--EIEREPLLFHVFSNAGVMLYRYVLELLQTHQRFRHLHVVGTIFDSGP--- 150

Query: 288 PDPQVWASGFSAAFLKKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEK 347
                                   S ++   R   +I      E +PAV     L++L  
Sbjct: 151 ----------------------GDSNLIGALRALATI-----LERRPAVLR---LLLLAA 210

Query: 348 FFGVV-----LNLPTVNRRLSDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQR 407
           F  VV     L  P      +   D L  S    P+LY+YS AD+V+ A  VE  +E + 
Sbjct: 211 FALVVILFHFLLAPFTALFHTHFYDRLQDSGSCWPELYLYSRADKVVSARDVERMVEARL 270

Query: 408 RAGREVRACNFVSTPHVDHYRNDPKLYSSQLSQFLADCV 440
                VR  +FVS+ HV H R+ P  Y+S    F+ +CV
Sbjct: 271 AHQVMVRGVDFVSSAHVSHLRDYPTYYTSLCVDFMHNCV 274

BLAST of Moc04g29880 vs. ExPASy Swiss-Prot
Match: Q2TBP5 (Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.6e-18
Identity = 80/272 (29.41%), Postives = 113/272 (41.54%), Query Frame = 0

Query: 172 VVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHV--DLLVNH 231
           +V+LLGW G   K+L +Y+  Y  RG   I +T P   +   +  G     V    L+  
Sbjct: 37  LVILLGWGGCSDKNLAKYSAIYHKRGCIVIRYTAPWHMVFFSETLGIPSLRVLAQKLLEL 96

Query: 232 LADWLEEEHGKNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQ 291
           L D+  E   + L+FH FSN G + Y  +LE  Q H      R+ G I DS P       
Sbjct: 97  LFDY--EVEKEPLLFHVFSNAGVMLYRYVLELLQTHQRFCHLRVVGTIFDSGP------- 156

Query: 292 VWASGFSAAFLKKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLM--VLEKFF 351
                               S +L   R    +      E +PA     LL+   L  F 
Sbjct: 157 ------------------GDSNLLGALRALAVV-----LEHRPAALRLLLLVAFTLVAFL 216

Query: 352 GVVLNLPTVNRRLSDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVR 411
             VL  P      +   D L  +    P+LY+YS AD V+ A  VE  +E +      VR
Sbjct: 217 FHVLLAPLTALFHTHFYDRLLDAASRWPELYLYSRADEVVLARDVERMVEARLAHQVLVR 276

Query: 412 ACNFVSTPHVDHYRNDPKLYSSQLSQFLADCV 440
           + +FVS+ HV H R+ P  Y++    F+  CV
Sbjct: 277 SVDFVSSAHVSHLRDYPTYYTTLCINFMHSCV 276

BLAST of Moc04g29880 vs. ExPASy Swiss-Prot
Match: Q6DHN0 (Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.6e-18
Identity = 72/273 (26.37%), Postives = 120/273 (43.96%), Query Frame = 0

Query: 172 VVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGG--KVEQHVDLLVNH 231
           VV+LLGW G++ KHL +Y+  Y  +G   + +T P+  +   +  G  ++      L+  
Sbjct: 29  VVILLGWAGSRDKHLAKYSSIYNEQGCTTLRYTAPLKTVFISESLGYKELRSTAHKLLEL 88

Query: 232 LADWLEEEHGKNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQ 291
           L D+  E     + FH FSN G++ Y  ++E    H       + G +VDSAP +     
Sbjct: 89  LYDY--EVENNPIFFHVFSNGGFMLYRYMVELLHSHKQFSTLCVVGTVVDSAPGS----- 148

Query: 292 VWASGFSAAFLKKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGV 351
              +   A    K ++  K + +L    +                   AL  V      +
Sbjct: 149 --QNVVGALRALKTTLGPKVNVLLQYFLL-------------------ALFAVAVFLLRI 208

Query: 352 VLNLPTVNRRLSDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRAC 411
           VL  P       +  D +       PQ+Y+YS ADRVI    VE  ++  +  G  V + 
Sbjct: 209 VL-YPLTKYFHRNHYDAMMEHPAPWPQMYLYSRADRVIRYRDVEKMVKGLQEKGLMVESF 268

Query: 412 NFVSTPHVDHYRNDPKLYSSQLSQFLADCVLTS 443
           +F++  HV  +R+ P+ YS++   FL+ C+ TS
Sbjct: 269 DFITPAHVSLFRDCPEDYSNRCRTFLSHCMTTS 272

BLAST of Moc04g29880 vs. ExPASy Swiss-Prot
Match: Q6DJC8 (Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.1e-18
Identity = 85/305 (27.87%), Postives = 125/305 (40.98%), Query Frame = 0

Query: 143 EVMYRWHLPEPTSLDISGSSACSVAKSR-TVVVLLGWLGAKQKHLKRYAEWYTSRGFHAI 202
           E+ Y    PEP+         C     R  VV+LLGW G K ++L +Y+  Y ++G   I
Sbjct: 5   ELDYTIEFPEPS------LQGCPWDPEREPVVILLGWGGCKDQYLAKYSAIYHNQGCTVI 64

Query: 203 TFTFPMSEILTYQIGG--KVEQHVDLLVNHLADWLEEEHGKNLVFHTFSNTGWLTYGAIL 262
            +T   + +   +  G   + +    L+  L D+  E     ++FH FSN G++ Y  I+
Sbjct: 65  KYTAAWNAVFISESLGFSSLREDAKKLLELLFDY--EIEKSPILFHVFSNGGFMLYRYIV 124

Query: 263 EKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNSVATKGSTMLDNTRME 322
           E    H       + G I DSAP                   +N +   GS    +T + 
Sbjct: 125 ELLHSHCRLNKLHVVGTIFDSAPG-----------------NRNVI---GSVRALDTILR 184

Query: 323 VSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRLSDVL-----DMLSSSQPSC 382
            S  N            A   + L  F  +V+ L  V   ++  L     D +       
Sbjct: 185 TSTNN------------AIRFLALAAFAIMVIILRIVLYPVTKFLHENHYDAMKKDSSRW 244

Query: 383 PQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHYRNDPKLYSSQLSQF 440
           PQLY+YS AD +I    VES I  +RR      A +F  + HV H+R  P  YS   + F
Sbjct: 245 PQLYLYSRADPIISYIDVESMIAARRRCCLPTEALDFGKSEHVSHFRRFPHRYSEMCTSF 269

BLAST of Moc04g29880 vs. ExPASy Swiss-Prot
Match: Q5PPS7 (Transmembrane protein 53-A OS=Xenopus laevis OX=8355 GN=tmem53-a PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.8e-17
Identity = 80/301 (26.58%), Postives = 128/301 (42.52%), Query Frame = 0

Query: 142 SEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLGAKQKHLKRYAEWYTSRGFHAI 201
           SE+ Y    PEP+             +   VV+LLGW G K  +L +Y+  Y ++G   I
Sbjct: 4   SELDYTIEFPEPSLQGWPWD-----PEREPVVILLGWGGCKDHYLAKYSAIYHNQGCTVI 63

Query: 202 TFTFPMSEI-LTYQIG-GKVEQHVDLLVNHLADWLEEEHGKNLVFHTFSNTGWLTYGAIL 261
            +T     + +T  +G   + +    L+  L D+  E     +VFH FSN G++ Y  I+
Sbjct: 64  KYTAAWKAVFITESLGLNSLREDAKKLLELLFDY--EIEKSPIVFHVFSNGGFMLYRYIV 123

Query: 262 EKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFLKKNSVATKGSTMLDNTRME 321
           E    H       + G I DSAP                   +N + +  +       ++
Sbjct: 124 ELLHSHCPLNKLHVVGTIFDSAPG-----------------NRNVIGSVRA-------LD 183

Query: 322 VSIRNKDDKESK-PAVTEAALLMVLEKFFGVVLNLPTVNRRLSDVLDMLSSSQPSCPQLY 381
             +R   +K  +  A+   A+L+++ +    +L  P       +  D +       PQLY
Sbjct: 184 TILRTSTNKAFRFLALAAFAILVIILR----ILLYPLTRFLHENHYDAMKKDPSRWPQLY 243

Query: 382 IYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHYRNDPKLYSSQLSQFLADC 440
           +YS AD +I    VES I  +RR        +F  + HV H+R  P+ YS   + FL DC
Sbjct: 244 LYSRADPIISYLDVESMIAARRRRCLPTETLDFGKSEHVSHFRRFPQRYSEICTSFLRDC 269

BLAST of Moc04g29880 vs. ExPASy TrEMBL
Match: A0A6J1CIU0 (transmembrane protein 53 OS=Momordica charantia OX=3673 GN=LOC111011995 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.2e-252
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE
Sbjct: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ
Sbjct: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL
Sbjct: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY
Sbjct: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 449
           RNDPKLYSSQLSQFLADCVLTSCCRESC
Sbjct: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 448

BLAST of Moc04g29880 vs. ExPASy TrEMBL
Match: A0A1S3CN08 (transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103502788 PE=3 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 5.1e-217
Identity = 388/448 (86.61%), Postives = 414/448 (92.41%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSLSGILQRPF A+TAVA+ASFSSDFSDKLPSQ+  + ++SSS+S   S++ST DFLKE
Sbjct: 1   MGSLSGILQRPFVALTAVAIASFSSDFSDKLPSQKPTEDSSSSSSS---SSDSTLDFLKE 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           S T F SH SVSKLANLSFVTKIHAP+P  +FPIPTSS NYV NF TSLVSS VLSNLYQ
Sbjct: 61  SNTSFVSHTSVSKLANLSFVTKIHAPIPKFSFPIPTSSCNYVGNFPTSLVSSSVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTK SKQ A S K+ SPPSEVMYRWHLPEPTSLDI+GSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKGSKQVAPSPKLSSPPSEVMYRWHLPEPTSLDITGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPM+EILTYQ+GGKVEQH+DLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQVGGKVEQHIDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQ HDSS+MGRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQNHDSSIMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKG T  DNT MEVSI +K++ E KPAVTEAALL++LEK FGVVLNLPTVNRRL
Sbjct: 301 KKNSVATKGLTTWDNTGMEVSISDKENIEPKPAVTEAALLLLLEKIFGVVLNLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVLD LSSSQPSCPQLYIYSSADRVIPA SVESFIEEQRRAGREVRACNFVSTPHVDH+
Sbjct: 361 SDVLDTLSSSQPSCPQLYIYSSADRVIPAGSVESFIEEQRRAGREVRACNFVSTPHVDHF 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 449
           RNDPKLY++QLSQFLADCVLT+CCRESC
Sbjct: 421 RNDPKLYTTQLSQFLADCVLTACCRESC 445

BLAST of Moc04g29880 vs. ExPASy TrEMBL
Match: A0A0A0KMY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G352630 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.6e-216
Identity = 388/448 (86.61%), Postives = 414/448 (92.41%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSLSGILQRPF A+TAVAVASFSSDFSDKLPSQ+  +A++SSS+S   S++ST DFLKE
Sbjct: 1   MGSLSGILQRPFVALTAVAVASFSSDFSDKLPSQKPTEASSSSSSSSSSSSDSTLDFLKE 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           S T F SH SVSKLANLSFVTKIHAP+P  +F  P+SS NYV NF TSLVSS VLSNLYQ
Sbjct: 61  SNTSFVSHTSVSKLANLSFVTKIHAPIPKFSF--PSSSCNYVGNFPTSLVSSSVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTK SKQAA S K+ SPPSEVMYRWHLPEPTSLDI+GSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKGSKQAAPSPKLSSPPSEVMYRWHLPEPTSLDITGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPM+EILTYQ+GGKVEQH+DLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLGGKVEQHIDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQ HDSS+MGRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQNHDSSIMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKG T  DNT MEVSI +K++ + KPAVTEAALL+VLEK FGVVLNLPTVNRRL
Sbjct: 301 KKNSVATKGLTTWDNTGMEVSISDKENIKPKPAVTEAALLLVLEKIFGVVLNLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVLD LSSSQPSCPQLYIYSSAD+VIPA SVESFIEEQRRAGREVRACNFVSTPHVDH+
Sbjct: 361 SDVLDTLSSSQPSCPQLYIYSSADQVIPAGSVESFIEEQRRAGREVRACNFVSTPHVDHF 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 449
           RNDPKLY++QLSQFLADCVLTSCCRESC
Sbjct: 421 RNDPKLYTTQLSQFLADCVLTSCCRESC 446

BLAST of Moc04g29880 vs. ExPASy TrEMBL
Match: A0A6J1FIX5 (transmembrane protein 53-like OS=Cucurbita moschata OX=3662 GN=LOC111444683 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.2e-211
Identity = 379/448 (84.60%), Postives = 402/448 (89.73%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSLSGILQRPF AVTAVAVASFSSDFSDKLPSQR P+A   S + P  S+ S  DFLKE
Sbjct: 1   MGSLSGILQRPFVAVTAVAVASFSSDFSDKLPSQRPPEA---SFSFPPSSSESILDFLKE 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           S+T F SH  VSKLA LSFVTKIHAP+P  +FPIP SS NYV NF TSL SS VLSNLYQ
Sbjct: 61  SRTSFVSHTPVSKLAYLSFVTKIHAPIPKCSFPIPNSSCNYVGNFRTSLASSSVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTKASKQAA S  +  PPSEVMYRWHLPEPTS+DI+GSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKASKQAAPSRNLSFPPSEVMYRWHLPEPTSVDITGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHL RYAEWYTSRGFHAITFTFPM+EIL YQ+GGKVEQH+DLLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLNRYAEWYTSRGFHAITFTFPMAEILAYQLGGKVEQHIDLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQ HDSS++GRIKGCIVDSAP AAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQNHDSSLLGRIKGCIVDSAPFAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKG T LDNT ME+SI NK++ E KPAVTEAALL+VLEK FG VL LPTVNRRL
Sbjct: 301 KKNSVATKGLTTLDNTGMEISISNKENIEPKPAVTEAALLLVLEKIFGFVLKLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVL+ LSSSQPSCPQLYIYSSADRVIPA+SVESFIEEQRRAG EVRACNFVSTPHVDH+
Sbjct: 361 SDVLNTLSSSQPSCPQLYIYSSADRVIPADSVESFIEEQRRAGHEVRACNFVSTPHVDHF 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRESC 449
           RNDPKLY+SQLSQFLADC+ TSCCRESC
Sbjct: 421 RNDPKLYTSQLSQFLADCLFTSCCRESC 445

BLAST of Moc04g29880 vs. ExPASy TrEMBL
Match: A0A6J1H3N4 (transmembrane protein 53-like OS=Cucurbita moschata OX=3662 GN=LOC111459714 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 6.0e-210
Identity = 387/447 (86.58%), Postives = 405/447 (90.60%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRSPDAAASSSASPDLSNNSTFDFLKE 60
           MGSLSGILQRPF AVTAVAVAS SSDFSDKLPSQR P  AASSS+  DLS+NST DFLKE
Sbjct: 1   MGSLSGILQRPFIAVTAVAVASISSDFSDKLPSQR-PTEAASSSSFSDLSHNSTLDFLKE 60

Query: 61  SKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLYQ 120
           S+T FA    VSKL NLSFVTKIHAPV   +FPIPT+S +YVRN H SLVSS VLSNLYQ
Sbjct: 61  SRTSFA---FVSKLENLSFVTKIHAPVLKFSFPIPTTSCDYVRNLHASLVSSTVLSNLYQ 120

Query: 121 SAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWLG 180
           SAALTKASKQ A S K  SPPSEVMYRWHLPEPTSLDI GSSACSVAKSRTVVVLLGWLG
Sbjct: 121 SAALTKASKQVAPSPKFSSPPSEVMYRWHLPEPTSLDIRGSSACSVAKSRTVVVLLGWLG 180

Query: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHGK 240
           AKQKHLKRYAEWYTSRGFHAITFTFPM+EILTYQ+ GKVEQH+ LLVNHLADWLEEEHGK
Sbjct: 181 AKQKHLKRYAEWYTSRGFHAITFTFPMAEILTYQLDGKVEQHISLLVNHLADWLEEEHGK 240

Query: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300
           NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL
Sbjct: 241 NLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAFL 300

Query: 301 KKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRRL 360
           KKNSVATKG T LD+T MEV I +K+D   KPAVTE ALL+VLEK FGVVL LPTVNRRL
Sbjct: 301 KKNSVATKGLTTLDSTGMEVPIGSKEDIVPKPAVTETALLLVLEKIFGVVLKLPTVNRRL 360

Query: 361 SDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDHY 420
           SDVLD LSSSQPSCPQLYIYSSADRVIPA+SVESFI+EQR+AGREVRACNFVSTPHVDH+
Sbjct: 361 SDVLDTLSSSQPSCPQLYIYSSADRVIPADSVESFIKEQRQAGREVRACNFVSTPHVDHF 420

Query: 421 RNDPKLYSSQLSQFLADCVLTSCCRES 448
           RNDPKLY+SQLSQFLADCVLT CCRES
Sbjct: 421 RNDPKLYTSQLSQFLADCVLT-CCRES 442

BLAST of Moc04g29880 vs. TAIR 10
Match: AT3G19970.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 519.2 bits (1336), Expect = 3.2e-147
Identity = 270/448 (60.27%), Postives = 340/448 (75.89%), Query Frame = 0

Query: 1   MGSLSGILQRPFFAVTAVAVASFSSDFSDKLPSQRS-PDAAASSSASPDLSNNSTFDFLK 60
           MGSLSGI+QRP  A  AV  AS S+D S++  S RS    + S   +P +S       ++
Sbjct: 1   MGSLSGIIQRPLVAAAAVITASVSADVSERFSSLRSLVRGSESEQIAPSVSG-----LVQ 60

Query: 61  ESKTLFASHISVSKLANLSFVTKIHAPVPNINFPIPTSSGNYVRNFHTSLVSSPVLSNLY 120
           E ++L+ S +S SKLA+LSFV++I  PVPN++      + N   +  TS+ S   L ++Y
Sbjct: 61  EERSLWVSQVSASKLADLSFVSRICVPVPNVDL----LASNPSCSLATSVTSLSALRSVY 120

Query: 121 QSAALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSVAKSRTVVVLLGWL 180
           QSA L KASK  A +        ++ YRWHLPEP+++D+SGSS+C   K+RTVVVLLGWL
Sbjct: 121 QSAELAKASKPVAFTIGASLVVPDISYRWHLPEPSAVDMSGSSSCVSEKNRTVVVLLGWL 180

Query: 181 GAKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVDLLVNHLADWLEEEHG 240
           G+KQKHLK+YA+WYTS+G+H ITFT PM+EI++YQ+GGK E++++ LVNHLADWL+EE  
Sbjct: 181 GSKQKHLKKYADWYTSKGYHVITFTLPMNEIMSYQVGGKAEKNIESLVNHLADWLDEEQK 240

Query: 241 KNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDSAPVAAPDPQVWASGFSAAF 300
           KNLVFHTFSNTGWLTYGAILEKFQK DSS+MGR+KGCIVDSAPVAA DP VWASGFSAAF
Sbjct: 241 KNLVFHTFSNTGWLTYGAILEKFQKQDSSLMGRVKGCIVDSAPVAAADPTVWASGFSAAF 300

Query: 301 LKKNSVATKGSTMLDNTRMEVSIRNKDDKESKPAVTEAALLMVLEKFFGVVLNLPTVNRR 360
           LKK+SVATKGS         ++I      + KPA TE ALL+VLEKFF V+LNLP VNRR
Sbjct: 301 LKKSSVATKGSASSSYESNGINI-----SQPKPAATETALLLVLEKFFAVILNLPKVNRR 360

Query: 361 LSDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIEEQRRAGREVRACNFVSTPHVDH 420
           L+DVLD LS++QPSCPQLYIYSSADRVIP   VESFI EQR+AG EVRACNF+S+PHVDH
Sbjct: 361 LADVLDTLSTAQPSCPQLYIYSSADRVIPVEQVESFIVEQRKAGHEVRACNFISSPHVDH 420

Query: 421 YRNDPKLYSSQLSQFLADCVLTSCCRES 448
           +R++P+LY+++L+ F+ + VL SCC  S
Sbjct: 421 FRSNPELYTAELNHFMNNFVL-SCCHHS 433

BLAST of Moc04g29880 vs. TAIR 10
Match: AT2G18245.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 5.6e-67
Identity = 143/341 (41.94%), Postives = 205/341 (60.12%), Query Frame = 0

Query: 111 SSPVLSNLYQS----AALTKASKQAASSHKIFSPPSEVMYRWHLPEPTSLDISGSSACSV 170
           S P  +NL++S     +  +    A  ++   S P++ +  W+   P  ++  G++A   
Sbjct: 58  SLPANANLFRSLFFLTSTFRLENTAVHANPFRSEPNDGVAVWN-RAPRIVNGGGNAAIFG 117

Query: 171 AK--SRTVVVLLGWLGAKQKHLKRYAEWYTSRGFHAITFTFPMSEILTYQIGGKVEQHVD 230
                 T VVLLGWLGAK KHL+RY EWY SRG +A+TFT  + ++L   +G ++E+ + 
Sbjct: 118 GNEGKETTVVLLGWLGAKAKHLRRYVEWYNSRGINAVTFTVDVRDLLRLDLGRRLERRIA 177

Query: 231 LLVNHLADWLEEEHG----KNLVFHTFSNTGWLTYGAILEKFQKHDSSVMGRIKGCIVDS 290
              N L +W+ E+      K LVFH+FSNTGWL YGA+LE F      ++ RIKGCI+DS
Sbjct: 178 EFGNELVNWVSEKEDDGREKCLVFHSFSNTGWLVYGALLESFIGR-QDLVERIKGCIIDS 237

Query: 291 APVAAPDPQVWASGFSAAFLKKNSVATKGSTMLDNTRMEVSIRNKD---DKESKPAVTEA 350
                 D ++WA+GF+AA LKK S     ST+   T     I+ +D    ++ +P   E 
Sbjct: 238 GGADPLDTKIWAAGFTAAILKKRS-----STI--TTEPNSPIKEEDASTPQKKEPLGIEN 297

Query: 351 ALLMVLEKFFGVVLNLPTVNRRLSDVLDMLSSSQPSCPQLYIYSSADRVIPANSVESFIE 410
            +L  LEK F + LN P VN RL+ ++  L  + P CPQLY+YSS D+V+P++SVE  I 
Sbjct: 298 IMLSSLEKLFPIFLNHPDVNTRLTKIIQKLYENHPPCPQLYLYSSGDKVVPSHSVELRIR 357

Query: 411 EQRRAGREVRACNFVSTPHVDHYRNDPKLYSSQLSQFLADC 439
           EQ++ GR++ + NF S+PHVDHYRN P LYSSQL  FL +C
Sbjct: 358 EQQKIGRKIHSFNFKSSPHVDHYRNFPDLYSSQLQNFLQEC 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141695.14.5e-252100.00transmembrane protein 53 [Momordica charantia] >XP_022141696.1 transmembrane pro... [more]
XP_008465096.11.0e-21686.61PREDICTED: transmembrane protein 53 [Cucumis melo] >XP_008465105.1 PREDICTED: tr... [more]
XP_004149868.21.2e-21586.61transmembrane protein 53 [Cucumis sativus] >XP_011655159.1 transmembrane protein... [more]
XP_038875313.11.1e-21385.71transmembrane protein 53 [Benincasa hispida] >XP_038875314.1 transmembrane prote... [more]
XP_023550651.11.9e-21385.71transmembrane protein 53-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9D0Z37.2e-1930.11Transmembrane protein 53 OS=Mus musculus OX=10090 GN=Tmem53 PE=2 SV=1[more]
Q2TBP53.6e-1829.41Transmembrane protein 53 OS=Bos taurus OX=9913 GN=TMEM53 PE=2 SV=1[more]
Q6DHN03.6e-1826.37Transmembrane protein 53 OS=Danio rerio OX=7955 GN=tmem53 PE=2 SV=1[more]
Q6DJC86.1e-1827.87Transmembrane protein 53-B OS=Xenopus laevis OX=8355 GN=tmem53-b PE=2 SV=1[more]
Q5PPS71.8e-1726.58Transmembrane protein 53-A OS=Xenopus laevis OX=8355 GN=tmem53-a PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CIU02.2e-252100.00transmembrane protein 53 OS=Momordica charantia OX=3673 GN=LOC111011995 PE=3 SV=... [more]
A0A1S3CN085.1e-21786.61transmembrane protein 53 OS=Cucumis melo OX=3656 GN=LOC103502788 PE=3 SV=1[more]
A0A0A0KMY95.6e-21686.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G352630 PE=3 SV=1[more]
A0A6J1FIX54.2e-21184.60transmembrane protein 53-like OS=Cucurbita moschata OX=3662 GN=LOC111444683 PE=3... [more]
A0A6J1H3N46.0e-21086.58transmembrane protein 53-like OS=Cucurbita moschata OX=3662 GN=LOC111459714 PE=3... [more]
Match NameE-valueIdentityDescription
AT3G19970.13.2e-14760.27alpha/beta-Hydrolases superfamily protein [more]
AT2G18245.15.6e-6741.94alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008547Protein of unknown function DUF829, TMEM53PFAMPF05705DUF829coord: 171..435
e-value: 6.6E-71
score: 239.0
IPR008547Protein of unknown function DUF829, TMEM53PANTHERPTHR12265UNCHARACTERIZEDcoord: 47..445
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 147..440
e-value: 3.1E-10
score: 41.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 168..437
NoneNo IPR availablePANTHERPTHR12265:SF30OS06G0730300 PROTEINcoord: 47..445

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g29880.1Moc04g29880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity