Moc04g27480 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g27480
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description(1->3)-beta-glucan endohydrolase
Locationchr4: 20302904 .. 20305685 (-)
RNA-Seq ExpressionMoc04g27480
SyntenyMoc04g27480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTTTCTGTGGGCAACATCACTAGTTTTAGTGGCAGTCACTCTTCTTTCCCATCAACTCCCTCTCATTAGTAAGAAACTTTCTCCGATACGATTCTATTTTTTATTAGTTATTTCTATTCTTTATCTCACCAATCAAACACATCCTTAATTTACATTGGCAGGCGGTTTGGGCGGTATCGGAGTGAACTACGGACTTAACAGCGACAATCTGCCGCAATCAAGTGATGTGATAAACTTGTACGGATGGTGTGACATCGATATCGTGCGGATATTTGAGCCGAACCAAATGCTCTGTGGTTGGGAACCAGAAATGAAGATATACACAGCTTCGCGACCGACCAAATGGCAGCCAATGCTTGGGTTAACGCTCACGTGCTTCCTTATTATGCAGATGTCAATATCGCATACATCACCGTTGGCAACGAAGTCGTTCCCGCCGACCCAGCTTCGCCTTTCGTTGCTAATGCCATTAGAAATATAATGCAAGCGCTTGTCATAACCGGCATCAAAAAGGACATCAAGGTGATATTTTAAATTAAAATTTAAATTACAAATTTGGTCGTTAAACTACCTATTAATTTTGTCCATATAATTTTAAAAGTTTCAATTTGATCTTAGATTAAGGTTTCGATTTAGTCCATATAATTTTAAATGGCCTAATTTAGTCTCTTAGATATGCACCATTAAGTTTTCCTTTTCCGGTCGCTTGTAATTTCATTAGTTTTACTTCTACTATATTAATAAAAAATGGATATATTAAAAACTTTGAACCATAACATTGGAAAATAAAGAATCAGGGTGTATCAATTTTTTTCTTTTTCAATTTAGTGGTGGTAGTTTTAAAAATTTTAGTTTAATCTTTATCGTTTGAGTTAATTAATTGGATTTTTGGGCTGAATAACCCTTTTACCAAGGCAGTATTGTTTTTTACCTACTTTATTCAAAACTTATGTTTTCTAATCTTTGCTGACATTACTTTGGATACCAATTTACTTGCATTATCCCTTTTTCCTCTCCCCCTTTTTCGAGTTTTTTTCTCTCATTTTCCATTAAAACTCATTCTTTCTCTTCCTTATTTTCTTCTCTTATTCTCTTTTCTGTAACACAATGGGTATTTTTCTCTCATTCTTCTATTTTGTTTTGGTTTCTCCTTTCCTTCTCTTTTTTTATTTTTTTAACTAACAGAAGGGTATAGAAGAGGGGAGAGGGGAGAGAGGAAGAAGGGAGAAGGAAGAGGAGAGAGGGGAAAGGGGGAAGAGAGGGCGAGAAGGAAGAAATGAGAGAGAGAGGAAGATGAGAGAGAGGAGAGAGGGTGAGAGGAAGAGGAGGGATAGAGAGAGGAGAGAGGAAGAGGAAGAGGAAGAGCGAGATCGAGATTTTGAGCGAGATCGAGATCGAGAGTAGAGATTTTGAGCGAGAGGAAAACGGCGAAGCCGACGGTAGTCAAAAAAGGAAAACGGAGCTGCCCACGGTCGGATCTCGCTCTCGACGGTTGGATCTCGCTCTCAGACGGTCTTTTATTTTTAGAGAGAGAAAGTGTGTCTATAAAATTAATTGTGTTTTTGTTCCATTCTAAATCTTATTTTTAGAGAGATAAAGTGTGTCTATGAAATTAATTGAACTTTTGTTCCATTCTACATCTTATTTTTAGAGGGAGAAAGTGTGTCAATAAAATTAATTGGGTTTTTGTTCTATTCTAAATCATATTTTTAGAGGGAGGGAGTCTATCAATTTATTTGGGTTCCATTATAAATCCTATTTTTAGATAGAGAAAGTATCTATGAAATTAATTGGGTTTTTGTTCCCTTCTAAATCATATTTTGAGAGAGAGAAAGTCTGAAGAGAACAAGAATGAGGAGAGGGGAAGAATGATGCTTTTTTGAAGATATGAAAAAATGGGGAGAGAAAACAAGAGAGAAATCTAAAAATGAGTGGAGGGATAGTAAAAGTAATTTGGTATCAAACATGATGTCAGCCAGATTAGAAAACATTTGGTAAAAGGGCTATTCAGCCCAAAAACCCTAATTAATTTTGCATTAGTAAAAATGATTTTAAAATTGGTCCATATAATTTGGACCTAGCTCTATTAAATTTTTGTGCTTTTAGTGTAGATTCAATTTAATCTCTTTATATTTATATAATCTTTATCTTTATTCATAAATTATAAATTTCCTTCGCCATAGGTAACCACGGTGGTCCATATGGGTGCAATGGGAGTCTCATATCCTCCATCCGCTGGAGCATTCACTGCCGCAGCGGCGGGACCTTTGAGCGACATCGCAAAAGTGGTGGCCTCCAACGATGCACCACTATTGTTGAACGTGTACCCATATTTTGCGTACGCATCAAGCCCACAACAAATCCCACTAAATTATGCCCTCTTCACTTCCACGACACCTGGAGTGGTGGATGGAAATCTGAACTACTACAATCTATTCGATGCCATGGTGGATTCTTTCTATGCAGCGCTGGAGAAGATTGGCGCTGCCAATGTCAGACTAGAGATTTCAGAGACTGGGTGGCCGACCGAAGGGAACGAGCCGTTCACCAGCATTGAGAATGCAGTGATGTATAACAAAAATTTCATGAACCATGTGGCGAGTGGAGCAGGAACTCTGAGGAGGCCAAATGTGAAGTACAATGGGATTTTGTTTGAGATGTTCAATGAGGATCTTAAGGCAGCAGGAGTTGAGCAAAATTTTGGCTTTCTTTTATCCCAATATGCAGCCAGTTTATCCATTTTGGAATTGCTAATTAAGGGATGGGGAAAAAGCTAA

mRNA sequence

ATGGACTTTCTGTGGGCAACATCACTAGTTTTAGTGGCAGTCACTCTTCTTTCCCATCAACTCCCTCTCATTAGCGGTTTGGGCGGTATCGGAGTGAACTACGGACTTAACAGCGACAATCTGCCGCAATCAAGTGATGTGATAAACTTGTACGGATGGTGTGACATCGATATCGTGCGGATATTTGAGCCGAACCAAATGCTCTGTGATGTCAATATCGCATACATCACCGTTGGCAACGAAGTCGTTCCCGCCGACCCAGCTTCGCCTTTCGTTGCTAATGCCATTAGAAATATAATGCAAGCGCTTGTCATAACCGGCATCAAAAAGGACATCAAGGTAACCACGGTGGTCCATATGGGTGCAATGGGAGTCTCATATCCTCCATCCGCTGGAGCATTCACTGCCGCAGCGGCGGGACCTTTGAGCGACATCGCAAAAGTGGTGGCCTCCAACGATGCACCACTATTGTTGAACGTGTACCCATATTTTGCGTACGCATCAAGCCCACAACAAATCCCACTAAATTATGCCCTCTTCACTTCCACGACACCTGGAGTGGTGGATGGAAATCTGAACTACTACAATCTATTCGATGCCATGGTGGATTCTTTCTATGCAGCGCTGGAGAAGATTGGCGCTGCCAATGTCAGACTAGAGATTTCAGAGACTGGGTGGCCGACCGAAGGGAACGAGCCGTTCACCAGCATTGAGAATGCAGTGATGTATAACAAAAATTTCATGAACCATGTGGCGAGTGGAGCAGGAACTCTGAGGAGGCCAAATGTGAAGTACAATGGGATTTTGTTTGAGATGTTCAATGAGGATCTTAAGGCAGCAGGAGTTGAGCAAAATTTTGGCTTTCTTTTATCCCAATATGCAGCCAGTTTATCCATTTTGGAATTGCTAATTAAGGGATGGGGAAAAAGCTAA

Coding sequence (CDS)

ATGGACTTTCTGTGGGCAACATCACTAGTTTTAGTGGCAGTCACTCTTCTTTCCCATCAACTCCCTCTCATTAGCGGTTTGGGCGGTATCGGAGTGAACTACGGACTTAACAGCGACAATCTGCCGCAATCAAGTGATGTGATAAACTTGTACGGATGGTGTGACATCGATATCGTGCGGATATTTGAGCCGAACCAAATGCTCTGTGATGTCAATATCGCATACATCACCGTTGGCAACGAAGTCGTTCCCGCCGACCCAGCTTCGCCTTTCGTTGCTAATGCCATTAGAAATATAATGCAAGCGCTTGTCATAACCGGCATCAAAAAGGACATCAAGGTAACCACGGTGGTCCATATGGGTGCAATGGGAGTCTCATATCCTCCATCCGCTGGAGCATTCACTGCCGCAGCGGCGGGACCTTTGAGCGACATCGCAAAAGTGGTGGCCTCCAACGATGCACCACTATTGTTGAACGTGTACCCATATTTTGCGTACGCATCAAGCCCACAACAAATCCCACTAAATTATGCCCTCTTCACTTCCACGACACCTGGAGTGGTGGATGGAAATCTGAACTACTACAATCTATTCGATGCCATGGTGGATTCTTTCTATGCAGCGCTGGAGAAGATTGGCGCTGCCAATGTCAGACTAGAGATTTCAGAGACTGGGTGGCCGACCGAAGGGAACGAGCCGTTCACCAGCATTGAGAATGCAGTGATGTATAACAAAAATTTCATGAACCATGTGGCGAGTGGAGCAGGAACTCTGAGGAGGCCAAATGTGAAGTACAATGGGATTTTGTTTGAGATGTTCAATGAGGATCTTAAGGCAGCAGGAGTTGAGCAAAATTTTGGCTTTCTTTTATCCCAATATGCAGCCAGTTTATCCATTTTGGAATTGCTAATTAAGGGATGGGGAAAAAGCTAA

Protein sequence

MDFLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVRIFEPNQMLCDVNIAYITVGNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAAGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTLRRPNVKYNGILFEMFNEDLKAAGVEQNFGFLLSQYAASLSILELLIKGWGKS
Homology
[Close]
BLAST of Moc04g27480 vs. NCBI nr
Match: XP_022152302.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 496.5 bits (1277), Expect = 1.7e-136
Identity = 263/330 (79.70%), Postives = 267/330 (80.91%), Query Frame = 0 

Query: 1   MDFLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVR 60
           MDFL ATSLVLVAV LLSHQLPLISGLGGIGVNYGLN DNLPQ SDVINLYG C IDIVR
Sbjct: 1   MDFLSATSLVLVAVALLSHQLPLISGLGGIGVNYGLNGDNLPQPSDVINLYGRCGIDIVR 60

Query: 61  IFEPNQ------------------------------------------MLCDVNIAYITV 120
           IFEPN                                              DVNI YITV
Sbjct: 61  IFEPNHGVLDALHGKPNALWLGTRNEDIHSFATDQMAANAWVNAHVLPYYADVNIEYITV 120

Query: 121 GNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAA 180
           GNEVVPADPA+PFVANAIRNIMQALV  GIKKDIKVTTVVHMGAMGVSYPPSAG FT AA
Sbjct: 121 GNEVVPADPATPFVANAIRNIMQALVTAGIKKDIKVTTVVHMGAMGVSYPPSAGVFTVAA 180

Query: 181 AGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLF 240
           AGPLSDIAKVV S+  PLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLF
Sbjct: 181 AGPLSDIAKVVGSSGTPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLF 240

Query: 241 DAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTL 289
           DAMVDSFYAALEKI AANVRLEISETGWPT+GNEPFTS+ENAV YNKNFMNHVASGAGT 
Sbjct: 241 DAMVDSFYAALEKIDAANVRLEISETGWPTKGNEPFTSVENAVTYNKNFMNHVASGAGTP 300

[Close]
BLAST of Moc04g27480 vs. NCBI nr
Match: XP_022141986.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 457.2 bits (1175), Expect = 1.1e-124
Identity = 242/330 (73.33%), Postives = 260/330 (78.79%), Query Frame = 0 

Query: 1   MDFLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVR 60
           MD L ATSLVL+A  +L HQLPLISGLGGIGVNYGLN DNLPQ +DVI+LYG C IDIVR
Sbjct: 1   MDLLRATSLVLMAAAVLCHQLPLISGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVR 60

Query: 61  IFEPNQ------------------------------------------MLCDVNIAYITV 120
           IFEPN                                              DVNIAYITV
Sbjct: 61  IFEPNHGVLDAMRGKPNALWLGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITV 120

Query: 121 GNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAA 180
           GNEV+PADPA+PFVANAIRNIMQAL ITG+KK IKVTTVV M AMGVSYPPSAG FTA A
Sbjct: 121 GNEVIPADPAAPFVANAIRNIMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVA 180

Query: 181 AGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLF 240
            GPLS+IAKVV S+ APLLLNVYPYFAYAS+PQQI LNYALFTSTTP +VDGNL+YYNLF
Sbjct: 181 LGPLSNIAKVVGSSGAPLLLNVYPYFAYASNPQQISLNYALFTSTTP-IVDGNLHYYNLF 240

Query: 241 DAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTL 289
           DAMVDSFYAALEKIGA NV LEISETGWPT+GNEPFTS+ENAV YNKNF+NHV+SGAGT 
Sbjct: 241 DAMVDSFYAALEKIGATNVTLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTP 300

[Close]
BLAST of Moc04g27480 vs. NCBI nr
Match: XP_022135630.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 453.0 bits (1164), Expect = 2.1e-123
Identity = 234/320 (73.12%), Postives = 253/320 (79.06%), Query Frame = 0 

Query: 11  LVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVRIFEPNQML-- 70
           +    LLSHQLPLI GLGGIGVNYGLN +NLPQ++DVINLYG C IDIVRIFEPN  +  
Sbjct: 1   MATALLLSHQLPLICGLGGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLD 60

Query: 71  ----------------------------------------CDVNIAYITVGNEVVPADPA 130
                                                    DVNIAYITVGNEV+P D A
Sbjct: 61  ALRGKPNVLWLGTRNEDIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAA 120

Query: 131 SPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAAGPLSDIAKV 190
           +PFVANAIRNIMQALV+TG+K+DIKVTTVVHM  +GVSYPPS GAFT AAAGPLS+IAKV
Sbjct: 121 APFVANAIRNIMQALVVTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKV 180

Query: 191 VASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAA 250
           V S+ APLLLNVYPYFAYAS+PQQIPLNYALFTSTTP VVDGNLNYYNLFDAMVDSFYAA
Sbjct: 181 VGSSGAPLLLNVYPYFAYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAA 240

Query: 251 LEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTLRRPNVKYNGI 289
           LEKIGA +VRLEISETGWPT+GN+PFTSI+NAV YNKNFMNHVASGAGT +RP VKYNGI
Sbjct: 241 LEKIGAVDVRLEISETGWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGI 300

[Close]
BLAST of Moc04g27480 vs. NCBI nr
Match: KAG6591760.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 399.1 bits (1024), Expect = 3.6e-107
Identity = 209/330 (63.33%), Postives = 241/330 (73.03%), Query Frame = 0 

Query: 1   MDFLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVR 60
           MDF    S  L+ + + S Q  LISGLG +GVNYGLN DNLP +S+V+ LYG C IDIVR
Sbjct: 1   MDFFRPPSAALLLLFIFSDQALLISGLGAVGVNYGLNGDNLPVASEVVELYGRCGIDIVR 60

Query: 61  IFEPNQMLCD-------------------------------------------VNIAYIT 120
           +FEPN  + D                                           VNIAYIT
Sbjct: 61  VFEPNHWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIAYIT 120

Query: 121 VGNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAA 180
           VGNEVVP D A+PFVANAI+NIMQALVI G+K DIKVTTVV M  + VSYPPSAGAF+A 
Sbjct: 121 VGNEVVPDDAAAPFVANAIKNIMQALVIAGVKSDIKVTTVVPMTPLEVSYPPSAGAFSAT 180

Query: 181 AAGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNL 240
           A GPL DI +V+ ++ APLLLNVYPYFAYAS+PQQI L+YALFTSTTP VVDGNL Y+NL
Sbjct: 181 AIGPLKDITQVLGTSGAPLLLNVYPYFAYASNPQQISLDYALFTSTTPVVVDGNLQYFNL 240

Query: 241 FDAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGT 288
           FDAMVDSFY+ALEKIGA  VR++ISETGWP++GN+PFTS+ENA  YN+NF+ HV SGAGT
Sbjct: 241 FDAMVDSFYSALEKIGAEGVRIDISETGWPSKGNDPFTSVENAATYNRNFVEHVRSGAGT 300

[Close]
BLAST of Moc04g27480 vs. NCBI nr
Match: XP_023536560.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 396.7 bits (1018), Expect = 1.8e-106
Identity = 208/334 (62.28%), Postives = 240/334 (71.86%), Query Frame = 0 

Query: 1   MDFLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVR 60
           MDF    S  L+ + + S Q  LISGLG +GVNYGLN DNLP  S+V+ LYG C IDIVR
Sbjct: 1   MDFFRPPSAALLLLFIFSDQALLISGLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDIVR 60

Query: 61  IFEPNQMLCD-------------------------------------------VNIAYIT 120
           +FEPN  + D                                           VNIAYIT
Sbjct: 61  VFEPNHWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIAYIT 120

Query: 121 VGNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAA 180
           VGNEVVP D A+PFVANAI+NIMQALVI G+K DIKVTTVV M  + VSYPPSAGAF+A 
Sbjct: 121 VGNEVVPDDAAAPFVANAIKNIMQALVIAGVKSDIKVTTVVPMTPLEVSYPPSAGAFSAT 180

Query: 181 AAGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNL 240
           A GPL DI +V+ ++ APLLLNVYPYFAYAS+PQQI L+YALFTSTTP VVDGNL Y+NL
Sbjct: 181 AIGPLKDITRVLGTSGAPLLLNVYPYFAYASNPQQISLDYALFTSTTPVVVDGNLQYFNL 240

Query: 241 FDAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGT 292
           FDAMVDSFY+ALEKIGA  VR++ISETGWP++GN+P TS+ENA  YN+NF+ HV SGAGT
Sbjct: 241 FDAMVDSFYSALEKIGAEGVRIDISETGWPSKGNDPLTSVENAATYNRNFVEHVRSGAGT 300

[Close]
BLAST of Moc04g27480 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-60
Identity = 131/300 (43.67%), Postives = 178/300 (59.33%), Query Frame = 0 

Query: 30  IGVNYGLNSDNLPQSSDVINLYGWCDIDIVRIFEPN------------QMLCD------- 89
           IGV YG+  +NLP  S+V++LY   +I  +R+++PN            Q+L D       
Sbjct: 29  IGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDVQ 88

Query: 90  -----------------------VNIAYITVGNEVVPADPASPFVANAIRNIMQALVITG 149
                                  V+  YI VGNE++P    + ++  A+RNI  AL   G
Sbjct: 89  SLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSAG 148

Query: 150 IKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAAGPLSDIAKVVASNDAPLLLNVYPYFAYA 209
           ++  IKV+T V  G +G SYPPSAGAF++AA   LS I + +ASN APLL+NVYPYF+Y 
Sbjct: 149 LQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFSYT 208

Query: 210 SSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAALEKIGAANVRLEISETGWP 269
            +P QI L YALFT++   V DG  +Y NLFDA+VD+ +AALE++G ANV + +SE+GWP
Sbjct: 209 GNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESGWP 268

Query: 270 TEGNEPFTSIENAVMYNKNFMNHVASGAGTLRRPNVKYNGILFEMFNEDLKAAGVEQNFG 288
           + G     S  NA  YN+N + HV  G GT RRP  +    +FEMFNE+ KA G+EQNFG
Sbjct: 269 SAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQNFG 326

[Close]
BLAST of Moc04g27480 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 132/328 (40.24%), Postives = 180/328 (54.88%), Query Frame = 0 

Query: 3   FLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVRIF 62
           FL+  S +++ V   S+    ++    IG+NYGL  DNLP  S+VINLY    I  +RIF
Sbjct: 9   FLFFLSCIVLYVN--SNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIF 68

Query: 63  EPN-------------------------------------------QMLCDVNIAYITVG 122
           +PN                                             L DVNI +ITVG
Sbjct: 69  DPNTEVLNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVG 128

Query: 123 NEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAA 182
           NEV+P  P  P V   ++++    ++      I ++TVV M  +  SYPPSAG FT+ A 
Sbjct: 129 NEVIPG-PIGPQVLPVMQSLTN--LVKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAR 188

Query: 183 GPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFD 242
             L  + K+++    P+L+N+YPYFAYAS P  I L+YA F + +  V DG+L Y N+FD
Sbjct: 189 EQLVPVLKLLSQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFD 248

Query: 243 AMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTLR 288
           A+ D+F  A+EK G  N+ + +SETGWP+ GN  FT+   A  YN+NF+ H+ASG GT +
Sbjct: 249 AIFDAFVWAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPK 308

[Close]
BLAST of Moc04g27480 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-53
Identity = 126/335 (37.61%), Postives = 180/335 (53.73%), Query Frame = 0 

Query: 3   FLWATSLVLVAVTL-------LSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCD 62
           FL+  S ++V V          ++ +  ++    IG+NYGL  DNLP  S VI LY   D
Sbjct: 9   FLFFFSCIVVIVNYNNSDFVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSID 68

Query: 63  IDIVRIFEPN-------------------------------------------QMLCDVN 122
           I  +RIF+PN                                             L D+N
Sbjct: 69  ITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDIN 128

Query: 123 IAYITVGNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAG 182
           IA+ITVGNEV+P  P  P V   ++++    ++      I ++TVV M  +  SYPPSAG
Sbjct: 129 IAFITVGNEVIPG-PIGPQVLPVMQSLTN--LVKSRNLPISISTVVAMWNLEQSYPPSAG 188

Query: 183 AFTAAAAGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNL 242
            FT+ A   L  + K+++  ++P+L+ +YPYF+YAS P  I L+YA F +    V DG+L
Sbjct: 189 MFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSL 248

Query: 243 NYYNLFDAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVA 288
            Y N+FDA+ D+F  A+EK G  ++ + +SETGWP+ GN   T+ + A  YN+NF+ H+A
Sbjct: 249 GYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIA 308

[Close]
BLAST of Moc04g27480 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.3e-46
Identity = 115/308 (37.34%), Postives = 161/308 (52.27%), Query Frame = 0 

Query: 24  ISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVRIFEPNQMLCD------------- 83
           ++G+ GIGVNYG+   +LP    V+ LY   +I  VRIF P+  + +             
Sbjct: 1   LAGVEGIGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEALRNSGLGVVLGT 60

Query: 84  -----------------------------VNIAYITVGNEVVPADPASPFVANAIRNIMQ 143
                                        V+  YI  GNEV+P + A+  V  A++N+  
Sbjct: 61  LNSDLAPLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAA-LVLPAMKNLEA 120

Query: 144 ALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAAGPLSDIAKVVASNDAPLLLNVY 203
           AL   G+   + VTT +    +G SYPPS G F+ AA   +  I   +AS+  PLL+NVY
Sbjct: 121 ALQAAGL--SVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNVY 180

Query: 204 PYFAYASSPQQIPLNYALFTSTTP-GVVDGNLNYYNLFDAMVDSFYAALEKIGAA-NVRL 263
           PYFAY++ P  + L+YAL +S+    V D  + Y N+FDA++D+ YAA+EK G   ++ L
Sbjct: 181 PYFAYSADPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLEL 240

Query: 264 EISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTLRRPNVKYNGILFEMFNEDLKA 288
            +SETGWP+ G     S+ENA  Y  N + HV    GT RRP       +F MFNE+ K 
Sbjct: 241 VVSETGWPSGGGGYGASVENAAAYINNLVRHV---GGTPRRPGKAVETYIFAMFNENQKP 300

[Close]
BLAST of Moc04g27480 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 8.2e-46
Identity = 116/327 (35.47%), Postives = 176/327 (53.82%), Query Frame = 0 

Query: 9   LVLVAVTLLSHQLPLISGL-------GGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVRI 68
           L L+++ LL  QL ++  L         IGV  G+  D+LP  ++V+ LY   +I  +R+
Sbjct: 13  LSLISIVLLLGQL-VVGSLATKQHTGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRL 72

Query: 69  FEPN-----------------------------------------QMLCDVNIAYITVGN 128
           ++PN                                         +   +V   YI VGN
Sbjct: 73  YDPNPAALEALRGSNIKLLLGVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGN 132

Query: 129 EVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAAG 188
           EV P+D  + F+  A+RNI +A+ + G+ K IKV+T +  G +G ++PPS G+F +    
Sbjct: 133 EVKPSDSFAQFLVPAMRNIQEAISLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNA 192

Query: 189 PLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDA 248
            L  I + + S+ +PLL+N+YPYFAY+ + Q I L+YALFT+ +  V DGN  Y NLFDA
Sbjct: 193 LLYPIIRFLVSHQSPLLVNLYPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDA 252

Query: 249 MVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTLRR 288
           M+D  YAALEK G  ++++ ISETGWP+      T+I+NA  +  N + HV    GT RR
Sbjct: 253 MLDGVYAALEKAGGGSLKVVISETGWPSAAGTA-TTIDNARTFISNLIQHVKE--GTPRR 312

[Close]
BLAST of Moc04g27480 vs. ExPASy TrEMBL
Match: A0A6J1DFU0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020050 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 8.0e-137
Identity = 263/330 (79.70%), Postives = 267/330 (80.91%), Query Frame = 0 

Query: 1   MDFLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVR 60
           MDFL ATSLVLVAV LLSHQLPLISGLGGIGVNYGLN DNLPQ SDVINLYG C IDIVR
Sbjct: 1   MDFLSATSLVLVAVALLSHQLPLISGLGGIGVNYGLNGDNLPQPSDVINLYGRCGIDIVR 60

Query: 61  IFEPNQ------------------------------------------MLCDVNIAYITV 120
           IFEPN                                              DVNI YITV
Sbjct: 61  IFEPNHGVLDALHGKPNALWLGTRNEDIHSFATDQMAANAWVNAHVLPYYADVNIEYITV 120

Query: 121 GNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAA 180
           GNEVVPADPA+PFVANAIRNIMQALV  GIKKDIKVTTVVHMGAMGVSYPPSAG FT AA
Sbjct: 121 GNEVVPADPATPFVANAIRNIMQALVTAGIKKDIKVTTVVHMGAMGVSYPPSAGVFTVAA 180

Query: 181 AGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLF 240
           AGPLSDIAKVV S+  PLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLF
Sbjct: 181 AGPLSDIAKVVGSSGTPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLF 240

Query: 241 DAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTL 289
           DAMVDSFYAALEKI AANVRLEISETGWPT+GNEPFTS+ENAV YNKNFMNHVASGAGT 
Sbjct: 241 DAMVDSFYAALEKIDAANVRLEISETGWPTKGNEPFTSVENAVTYNKNFMNHVASGAGTP 300

[Close]
BLAST of Moc04g27480 vs. ExPASy TrEMBL
Match: A0A6J1CKU8 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012224 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 5.4e-125
Identity = 242/330 (73.33%), Postives = 260/330 (78.79%), Query Frame = 0 

Query: 1   MDFLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVR 60
           MD L ATSLVL+A  +L HQLPLISGLGGIGVNYGLN DNLPQ +DVI+LYG C IDIVR
Sbjct: 1   MDLLRATSLVLMAAAVLCHQLPLISGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVR 60

Query: 61  IFEPNQ------------------------------------------MLCDVNIAYITV 120
           IFEPN                                              DVNIAYITV
Sbjct: 61  IFEPNHGVLDAMRGKPNALWLGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITV 120

Query: 121 GNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAA 180
           GNEV+PADPA+PFVANAIRNIMQAL ITG+KK IKVTTVV M AMGVSYPPSAG FTA A
Sbjct: 121 GNEVIPADPAAPFVANAIRNIMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVA 180

Query: 181 AGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLF 240
            GPLS+IAKVV S+ APLLLNVYPYFAYAS+PQQI LNYALFTSTTP +VDGNL+YYNLF
Sbjct: 181 LGPLSNIAKVVGSSGAPLLLNVYPYFAYASNPQQISLNYALFTSTTP-IVDGNLHYYNLF 240

Query: 241 DAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTL 289
           DAMVDSFYAALEKIGA NV LEISETGWPT+GNEPFTS+ENAV YNKNF+NHV+SGAGT 
Sbjct: 241 DAMVDSFYAALEKIGATNVTLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTP 300

[Close]
BLAST of Moc04g27480 vs. ExPASy TrEMBL
Match: A0A6J1C1J7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007535 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 1.0e-123
Identity = 234/320 (73.12%), Postives = 253/320 (79.06%), Query Frame = 0 

Query: 11  LVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVRIFEPNQML-- 70
           +    LLSHQLPLI GLGGIGVNYGLN +NLPQ++DVINLYG C IDIVRIFEPN  +  
Sbjct: 1   MATALLLSHQLPLICGLGGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLD 60

Query: 71  ----------------------------------------CDVNIAYITVGNEVVPADPA 130
                                                    DVNIAYITVGNEV+P D A
Sbjct: 61  ALRGKPNVLWLGTRNEDIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAA 120

Query: 131 SPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAAGPLSDIAKV 190
           +PFVANAIRNIMQALV+TG+K+DIKVTTVVHM  +GVSYPPS GAFT AAAGPLS+IAKV
Sbjct: 121 APFVANAIRNIMQALVVTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKV 180

Query: 191 VASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAA 250
           V S+ APLLLNVYPYFAYAS+PQQIPLNYALFTSTTP VVDGNLNYYNLFDAMVDSFYAA
Sbjct: 181 VGSSGAPLLLNVYPYFAYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAA 240

Query: 251 LEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTLRRPNVKYNGI 289
           LEKIGA +VRLEISETGWPT+GN+PFTSI+NAV YNKNFMNHVASGAGT +RP VKYNGI
Sbjct: 241 LEKIGAVDVRLEISETGWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGI 300

[Close]
BLAST of Moc04g27480 vs. ExPASy TrEMBL
Match: A0A0A0L3A5 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 2.3e-107
Identity = 209/331 (63.14%), Postives = 242/331 (73.11%), Query Frame = 0 

Query: 1   MDFLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVR 60
           MDF + TS +L+ ++L SHQ+P ISG G +GVNYGLN DNLP  SDVINLYG C I+I+R
Sbjct: 1   MDFFFPTSFLLL-LSLFSHQIPPISGFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILR 60

Query: 61  IFEPNQMLCD-------------------------------------------VNIAYIT 120
           +FEPN  + D                                           VNIAYIT
Sbjct: 61  LFEPNHGVLDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYIT 120

Query: 121 VGNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAA 180
           +GNEVVP D A+PFVAN I+NIMQALV  GIK DIKVTTVV M A+GVSYPPSAGAFT +
Sbjct: 121 IGNEVVPGDAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGS 180

Query: 181 AAGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNL 240
           AAG + DIA V+ S+ AP+L+NVYPYFAYAS+PQQI LNYALFTS+TP VVDGNL Y+NL
Sbjct: 181 AAGIMKDIANVLGSSGAPILVNVYPYFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNL 240

Query: 241 FDAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGT 289
           FDAMVDSFYAALEKI A  +R+ ISETGWPT GNEPFTS+ENA+ YNKN + HV SG GT
Sbjct: 241 FDAMVDSFYAALEKIDAGEIRIGISETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGT 300

[Close]
BLAST of Moc04g27480 vs. ExPASy TrEMBL
Match: A0A6J1F682 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442719 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.9e-106
Identity = 207/330 (62.73%), Postives = 239/330 (72.42%), Query Frame = 0 

Query: 1   MDFLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVR 60
           MDF    S  L+ + + S Q  LISGLG +GVNYGLN DNLP +SDV+ LYG C IDIVR
Sbjct: 1   MDFFRPPSAALLLLFIFSDQALLISGLGAVGVNYGLNGDNLPVASDVVELYGRCGIDIVR 60

Query: 61  IFEPNQMLCD-------------------------------------------VNIAYIT 120
           +FEPN  + D                                           VNIAYIT
Sbjct: 61  VFEPNHWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIAYIT 120

Query: 121 VGNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAA 180
           VGNEVVP D A+PFVANAI+NIMQALVI G+K DIKVTTVV M  + VSYPPSAGAF+A 
Sbjct: 121 VGNEVVPGDAAAPFVANAIKNIMQALVIAGVKSDIKVTTVVPMTPLEVSYPPSAGAFSAT 180

Query: 181 AAGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNL 240
           A GPL DI +V+ ++ APLLLNVYPYFAYAS+PQQI L+YALFTSTTP VVDGNL Y+NL
Sbjct: 181 AIGPLKDITQVLGTSGAPLLLNVYPYFAYASNPQQISLDYALFTSTTPVVVDGNLQYFNL 240

Query: 241 FDAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGT 288
           FDAMVDSFY+ALEKIGA  VR++ISETGWP++GN+  TS+ENA  YN+NF+ HV SGAGT
Sbjct: 241 FDAMVDSFYSALEKIGAERVRIDISETGWPSKGNDQLTSVENAATYNRNFVEHVRSGAGT 300

[Close]
BLAST of Moc04g27480 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 224.9 bits (572), Expect = 8.7e-59
Identity = 132/328 (40.24%), Postives = 180/328 (54.88%), Query Frame = 0 

Query: 3   FLWATSLVLVAVTLLSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVRIF 62
           FL+  S +++ V   S+    ++    IG+NYGL  DNLP  S+VINLY    I  +RIF
Sbjct: 9   FLFFLSCIVLYVN--SNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIF 68

Query: 63  EPN-------------------------------------------QMLCDVNIAYITVG 122
           +PN                                             L DVNI +ITVG
Sbjct: 69  DPNTEVLNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVG 128

Query: 123 NEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAA 182
           NEV+P  P  P V   ++++    ++      I ++TVV M  +  SYPPSAG FT+ A 
Sbjct: 129 NEVIPG-PIGPQVLPVMQSLTN--LVKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAR 188

Query: 183 GPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFD 242
             L  + K+++    P+L+N+YPYFAYAS P  I L+YA F + +  V DG+L Y N+FD
Sbjct: 189 EQLVPVLKLLSQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFD 248

Query: 243 AMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTLR 288
           A+ D+F  A+EK G  N+ + +SETGWP+ GN  FT+   A  YN+NF+ H+ASG GT +
Sbjct: 249 AIFDAFVWAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPK 308

[Close]
BLAST of Moc04g27480 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 9.6e-58
Identity = 128/321 (39.88%), Postives = 180/321 (56.07%), Query Frame = 0 

Query: 10  VLVAVTLLSHQLPLISGLGGI-GVNYGLNSDNLPQSSDVINLYGWCDIDIVRIFEP---- 69
           ++  +  LS +L     +    GV YG N DNLP  +  ++LY   ++  +R++EP    
Sbjct: 3   LIATLLFLSARLTTAGNMNSFAGVCYGRNGDNLPSPAKTVSLYKKINVGGIRLYEPFPDL 62

Query: 70  ----------------NQMLCDV-------------------NIAY--ITVGNEVVPADP 129
                           N+ +  +                   N+A+  ITVGNEV+  + 
Sbjct: 63  IVSLQGTGLLVAIGPRNEAIKTLAEEYQFALNWDKTFIAPYKNVAFNWITVGNEVIEGE- 122

Query: 130 ASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAAGPLSDIAK 189
              +V  A++NI  AL   G  K I VTTV+   A+  SYPPSAG F  A    +++I  
Sbjct: 123 IGRYVPQAMKNIKAALTEIGNSK-IHVTTVISTAALANSYPPSAGVFKPAITELITEIVS 182

Query: 190 VVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYA 249
           +++S D+PL++NVYPYFAYAS P  + L YA F ST+P V DG   Y N+FDA +D+F  
Sbjct: 183 ILSSTDSPLMVNVYPYFAYASDPSHVSLEYATFRSTSPVVTDGKYQYTNIFDATLDAFNV 242

Query: 250 ALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTLRRPNVKYNG 289
           ALEKI   +V++ ++ETGWPT GN+P+TS+ENA  YN+  +  + +G GT RRPNV    
Sbjct: 243 ALEKINHGSVKVYVAETGWPTRGNDPYTSVENARAYNQGLLKKLTTGKGTPRRPNVPVIT 302

[Close]
BLAST of Moc04g27480 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 211.8 bits (538), Expect = 7.6e-55
Identity = 126/335 (37.61%), Postives = 180/335 (53.73%), Query Frame = 0 

Query: 3   FLWATSLVLVAVTL-------LSHQLPLISGLGGIGVNYGLNSDNLPQSSDVINLYGWCD 62
           FL+  S ++V V          ++ +  ++    IG+NYGL  DNLP  S VI LY   D
Sbjct: 9   FLFFFSCIVVIVNYNNSDFVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSID 68

Query: 63  IDIVRIFEPN-------------------------------------------QMLCDVN 122
           I  +RIF+PN                                             L D+N
Sbjct: 69  ITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDIN 128

Query: 123 IAYITVGNEVVPADPASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAG 182
           IA+ITVGNEV+P  P  P V   ++++    ++      I ++TVV M  +  SYPPSAG
Sbjct: 129 IAFITVGNEVIPG-PIGPQVLPVMQSLTN--LVKSRNLPISISTVVAMWNLEQSYPPSAG 188

Query: 183 AFTAAAAGPLSDIAKVVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNL 242
            FT+ A   L  + K+++  ++P+L+ +YPYF+YAS P  I L+YA F +    V DG+L
Sbjct: 189 MFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSL 248

Query: 243 NYYNLFDAMVDSFYAALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNFMNHVA 288
            Y N+FDA+ D+F  A+EK G  ++ + +SETGWP+ GN   T+ + A  YN+NF+ H+A
Sbjct: 249 GYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIA 308

[Close]
BLAST of Moc04g27480 vs. TAIR 10
Match: AT1G77790.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 5.4e-53
Identity = 132/322 (40.99%), Postives = 176/322 (54.66%), Query Frame = 0 

Query: 12  VAVTLLSHQLPLISGLGG--IGVNYGLNSDNLPQSSDVINLYGWCDIDIVRIFEP----- 71
           V + LLS Q+   +G+ G   G  YG N +NLP  +D + LY   +ID +R++EP     
Sbjct: 10  VVIMLLSIQIFCTAGVAGDITGDCYGRNGNNLPTPADTVALYKSNNIDAIRMYEPFADML 69

Query: 72  ------------------NQMLC-------------------DVNIAYITVGNEVVPADP 131
                              Q L                    DV I +IT+GNEV P + 
Sbjct: 70  EALRGSGLLVAFGPRNEDIQSLAHDPAAATNFVSTWITPYQNDVAIKWITIGNEVFPGEI 129

Query: 132 ASPFVANAIRNIMQALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAAGPLSDIAK 191
           A  FVA AI+N+  AL  +G+   I VTTV+ M A+  +YPPSA  F       +++I  
Sbjct: 130 AQ-FVAAAIKNVNVALTNSGV-TGISVTTVLAMTALTNTYPPSAATFLPDLTEIMTEITS 189

Query: 192 VVASNDAPLLLNVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYA 251
           +++  ++PL+ N+YPYFAYAS P  I L+YA F S TP V+DG+L Y N+F+AMVD F A
Sbjct: 190 ILSETNSPLMTNIYPYFAYASDPYHISLDYASFKSNTPVVIDGDLYYNNMFEAMVDGFNA 249

Query: 252 ALEKIGAANVRLEISETGWPTEGNEPFTSIENAVMYNKNF--MNHVASGAGTLRRPNVKY 288
           ALEKI AANV + ++ETGWPTEGN P TS++NA  YN         A    T RR N   
Sbjct: 250 ALEKINAANVVVMVAETGWPTEGNPPHTSVDNAKAYNMGIRTCGRSAERKRTPRRQNTPV 309

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BLAST of Moc04g27480 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 205.3 bits (521), Expect = 7.1e-53
Identity = 116/303 (38.28%), Postives = 164/303 (54.13%), Query Frame = 0 

Query: 24  ISGLGGIGVNYGLNSDNLPQSSDVINLYGWCDIDIVRIFEP------------------- 83
           ++    IG+NYGL  DN P  S+V+NLY    I  +RIF+P                   
Sbjct: 28  VAAANSIGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNALRGHRNIEVTVG 87

Query: 84  ------------------------NQMLCDVNIAYITVGNEVVPADPASPFVANAIRNIM 143
                                      L DVNIA+ITVGNEV+P  P  P V   ++++ 
Sbjct: 88  VRDQDLGALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPG-PIGPQVLPVMQSL- 147

Query: 144 QALVITGIKKDIKVTTVVHMGAMGVSYPPSAGAFTAAAAGPLSDIAKVVASNDAPLLLNV 203
             +++  +   I ++TVV M  +  SYPPSAG FT+ A   L  + K+++    P+L+N+
Sbjct: 148 -TILVKSMNLPISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVLKLLSQTSTPILVNI 207

Query: 204 YPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAALEKIGAANVRLE 263
           YPYF YAS P  IPL+YA F +    V DG L Y N+FDA+ D+F  A+EK G  ++ + 
Sbjct: 208 YPYFPYASDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVWAMEKEGVKDLPMV 267

Query: 264 ISETGWPTEGNEPFTSIENAVMYNKNFMNHVASGAGTLRRPNVKYNGILFEMFNEDLKAA 284
           +SETGWP+ GN   T+ + A  YN+NF+ H+ SG GT +RPN   +G LF  FNE+ K A
Sbjct: 268 VSETGWPSAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMDGFLFATFNENQKPA 327

The following BLAST results are available for this feature:
BLAST of Moc04g27480 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022152302.11.7e-13679.70probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia][more]
XP_022141986.11.1e-12473.33probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia][more]
XP_022135630.12.1e-12373.13probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia][more]
KAG6591760.13.6e-10763.33Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023536560.11.8e-10662.28probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo][more]
BLAST of Moc04g27480 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O223171.5e-6043.67Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2[more]
O493521.2e-5740.24Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN... [more]
O493531.1e-5337.61Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q024396.3e-4637.34Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4... [more]
P524088.2e-4635.47Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS... [more]
BLAST of Moc04g27480 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DFU08.0e-13779.70(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020050 ... [more]
A0A6J1CKU85.4e-12573.33(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012224 ... [more]
A0A6J1C1J71.0e-12373.13(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007535 ... [more]
A0A0A0L3A52.3e-10763.14(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3... [more]
A0A6J1F6821.9e-10662.73(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442719 P... [more]
BLAST of Moc04g27480 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G20330.18.7e-5940.24beta-1,3-glucanase 4 [more]
AT1G77780.19.6e-5839.88Glycosyl hydrolase superfamily protein [more]
AT5G20340.17.6e-5537.61beta-1,3-glucanase 5 [more]
AT1G77790.15.4e-5340.99Glycosyl hydrolase superfamily protein [more]
AT1G33220.17.1e-5338.28Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 69..292
e-value: 1.5E-62
score: 212.0
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 66..299
e-value: 3.5E-75
score: 254.9
NoneNo IPR availablePANTHERPTHR32227:SF248GLYCOSYL HYDROLASE SUPERFAMILY PROTEINcoord: 8..65
NoneNo IPR availablePANTHERPTHR32227:SF248GLYCOSYL HYDROLASE SUPERFAMILY PROTEINcoord: 71..291
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 8..65
coord: 71..291
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 30..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g27480.1Moc04g27480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds