Moc04g11400 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g11400
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Reverse transcriptase
Location	chr4: 8587023 .. 8588477 (+)
RNA-Seq Expression	Moc04g11400
Synteny	Moc04g11400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGCTATCAACTCGGCAGCCCTCCCCATCATGGGAGTTGCTAAGAGAGTCTTAGTAAAACTAGGGACATGGAGTGAACAAGTCGATTTTATGATAGTGCAGGTGGATGACTTTGACGTAGTATTGGAAATGGAATTTCTTCTAGAATACAAAGTCATCCCCATGCCTCTCGCCAAGTGCCTAGTTGAAACGGGTTCCGACTCCACGGTTGTTCCGACAAGCATCAAACAACCAAGTGGAGTGAAGATGATATCAGCGCTCCAACTAAATAAAGGTCTTGCTAATGATGAACCAACATTCATGGCCATTCCTGTAGTGGAAGACAGAAACTCTAAAGAACCAGTTCCCCGAGAAATCCAACAGGTCTTAGATGTATCTGTAGATCGTAATGCCAGATAGCCTGTCCAAGGCCCTACCTCTAAGACGGGGGATAGACCATGAGATTGAGCTGTTACCTGGGGCAAAATTGCCGACCAAGAATGCCTATCGGATGGCTCCTCCTGAGTTAGCTGAGCTAAGGAAACAACTCGATGAATTATTGAACGCTGGATTCATCCGTCTATGCATTGATTATCGAGCATTGAACAAGCTCGCAGTCTGCAACAAGTACCCTTTACCCATCATCACAAACCTTTTTTATCAGCTTCATGGGGCAAATACTTCTCTAAACTGGATCTATGATTTGGGTACTATCAAGTGTGCATTGCAGAAGGAGACGAACCGAAGACTACATGCGTCACTCGGTATGGGGTCTTTGAATTCCTTGTCATGCCCTTTGGTCTCACGAACACTCCTGCCACCTTCTACACCTTAATAAACCAGGTGTCTCACGAATACCTCGACAAGTTTGTGGTGGAATATCTAGATGACATAGTTGTTTATAGCCCAACCATGGAAGAACACCAGATACACTTGCAACTTGTTTTCGAGAAGCTCAGACAGAACTAGTTGTATGTCAAGCGGGAAAAGTGTTCTTTCACTCAAGAGCACATCTGTTTCCCAGGCCATGTCATAGAATGTGGCCGAATAAGCATGGAAGATGGGAAGATCAAAGCCATTCAAGAATGAAAAGTGCCGACATCCATCACAGAACTACATTCATTTTTCGGTCTAGCCAACTACTATATGCGATTTGTAGAAGGATTCTCGAAAAAGGCAGGACCCTTGACTGAGATACTAAAGAAGAATCAATCGTGGAATTGGACCTCAGAGTGTCAACCCGCATTCGAGAGCTTGAAAAAGGAAATGATGGAGAGGCCGATGTTGGGGATCACAGATGTTGCAAGACCGTTTGAAGTCGAGACTGATGCTTCAGACTTTTCTCTGGGAGGAGTGCTTCTCCAAGACAACCACCCCATTGCATACGAGAGTCGAAAATTGAATGATGCCGAAAAGAGGTATGCTGCCTCCGGGAATGATATGTTAGCATTAGTCCACTACTTGAGGGCCTGA

mRNA sequence

ATGAAAGCTATCAACTCGGCAGCCCTCCCCATCATGGGAGTTGCTAAGAGAGTCTTAGTAAAACTAGGGACATGGAGTGAACAAGTCGATTTTATGATAGTGCAGGTGGATGACTTTGACGTAGTATTGGAAATGGAATTTCTTCTAGAATACAAAGTCATCCCCATGCCTCTCGCCAAGTGCCTAGTTGAAACGGGTTCCGACTCCACGGTTGTTCCGACAAGCATCAAACAACCAAGTGGAGTGAAGATGATATCAGCGCTCCAACTAAATAAAGGTCTTGCTAATGATGAACCAACATTCATGGCCATTCCTGTAGTGGAAGACAGAAACTCTAAAGAACCAGTTCCCCGAGAAATCCAACAGGTCTTAGATGTATCTGTAGATCGACCCTTGACTGAGATACTAAAGAAGAATCAATCGTGGAATTGGACCTCAGAGTGTCAACCCGCATTCGAGAGCTTGAAAAAGGAAATGATGGAGAGGCCGATGTTGGGGATCACAGATGTTGCAAGACCGTTTGAAGTCGAGACTGATGCTTCAGACTTTTCTCTGGGAGGAGTGCTTCTCCAAGACAACCACCCCATTGCATACGAGAGTCGAAAATTGAATGATGCCGAAAAGAGGTATGCTGCCTCCGGGAATGATATGTTAGCATTAGTCCACTACTTGAGGGCCTGA

Coding sequence (CDS)

ATGAAAGCTATCAACTCGGCAGCCCTCCCCATCATGGGAGTTGCTAAGAGAGTCTTAGTAAAACTAGGGACATGGAGTGAACAAGTCGATTTTATGATAGTGCAGGTGGATGACTTTGACGTAGTATTGGAAATGGAATTTCTTCTAGAATACAAAGTCATCCCCATGCCTCTCGCCAAGTGCCTAGTTGAAACGGGTTCCGACTCCACGGTTGTTCCGACAAGCATCAAACAACCAAGTGGAGTGAAGATGATATCAGCGCTCCAACTAAATAAAGGTCTTGCTAATGATGAACCAACATTCATGGCCATTCCTGTAGTGGAAGACAGAAACTCTAAAGAACCAGTTCCCCGAGAAATCCAACAGGTCTTAGATGTATCTGTAGATCGACCCTTGACTGAGATACTAAAGAAGAATCAATCGTGGAATTGGACCTCAGAGTGTCAACCCGCATTCGAGAGCTTGAAAAAGGAAATGATGGAGAGGCCGATGTTGGGGATCACAGATGTTGCAAGACCGTTTGAAGTCGAGACTGATGCTTCAGACTTTTCTCTGGGAGGAGTGCTTCTCCAAGACAACCACCCCATTGCATACGAGAGTCGAAAATTGAATGATGCCGAAAAGAGGTATGCTGCCTCCGGGAATGATATGTTAGCATTAGTCCACTACTTGAGGGCCTGA

Protein sequence

MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAKCLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREIQQVLDVSVDRPLTEILKKNQSWNWTSECQPAFESLKKEMMERPMLGITDVARPFEVETDASDFSLGGVLLQDNHPIAYESRKLNDAEKRYAASGNDMLALVHYLRA

Homology

[Close]

BLAST of Moc04g11400 vs. NCBI nr
Match: XP_022155185.1 (uncharacterized protein LOC111022320 [Momordica charantia])

HSP 1 Score: 248.1 bits (632), Expect = 7.5e-62
Identity = 176/463 (38.01%), Postives = 195/463 (42.12%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MKA+NSAALPIMGVAKRV VKLGTWS  VDF+IV++DDFDVVL ++FLLE+KVIPMPLAK
Sbjct: 470 MKAVNSAALPIMGVAKRVSVKLGTWSGLVDFVIVRMDDFDVVLGIKFLLEHKVIPMPLAK 529

Query: 61  CLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREI 120
           CLV T SD  VV TSIKQPSGVKMISALQL KG+A DEPTFMAIPV E RNS+E VPREI
Sbjct: 530 CLVVTSSDPIVVQTSIKQPSGVKMISALQLKKGIAQDEPTFMAIPVAEGRNSEELVPREI 589

Query: 121 QQVLDVSVD--------------------------------------------------- 180
           Q+VL+   D                                                   
Sbjct: 590 QRVLEAYADVMPDNLPKNLPPRPGIDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDEL 649

Query: 181 ------------------------------------------------------------ 227
                                                                       
Sbjct: 650 LNAGFIRPAKAPYGAPVLFQKKKDXSLRLCIDYRALNKLXVRNKXPLPIITDLFDQLHGA 709

[Close]

BLAST of Moc04g11400 vs. NCBI nr
Match: KAA0041498.1 (uncharacterized protein E6C27_scaffold6G00890 [Cucumis melo var. makuwa] >TYK24371.1 uncharacterized protein E5676_scaffold205G001550 [Cucumis melo var. makuwa])

HSP 1 Score: 213.0 bits (541), Expect = 2.7e-51
Identity = 142/406 (34.98%), Postives = 180/406 (44.33%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MK +NSAALPI+G+ KR +++LG WS  VDF++V++DDFDVVL MEFLLE++VIPMPLAK
Sbjct: 375 MKVVNSAALPIIGLVKRTMIRLGGWSGLVDFVVVKIDDFDVVLAMEFLLEHQVIPMPLAK 434

Query: 61  CLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREI 120
           CLV TGS  + V T ++QP G+KMISA +L KGL+ DEPTFMAIP+    NS E VP+EI
Sbjct: 435 CLVITGSTPSFVQTDLRQPDGLKMISARKLKKGLSQDEPTFMAIPLNSSENSGETVPKEI 494

Query: 121 QQVL----DVSVD----------------------------------------------- 180
            +VL    DV  D                                               
Sbjct: 495 MRVLEKYRDVIPDSLPKSLPPRRMIDHEIKLVPRAKPPVKNSYRFIRPTKAPYGAPVLFQ 554

Query: 181 ------------------------------------------------------------ 227
                                                                       
Sbjct: 555 KKKDGSLRLCIDYRVLNKLTVRNKYPLPIITNLFDRLHEAKYFSKLDLRSGYYQVRIAEG 614

[Close]

BLAST of Moc04g11400 vs. NCBI nr
Match: KAA0042379.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK15498.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 206.1 bits (523), Expect = 3.3e-49
Identity = 144/421 (34.20%), Postives = 177/421 (42.04%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MKA+NSAALPI+G+ KR +++LG WS  VDF++V++DDFDVVL MEFLLE++VIPMPLAK
Sbjct: 489 MKAVNSAALPIIGLVKRTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHRVIPMPLAK 548

Query: 61  CLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREI 120
           CLV TG   +VV T ++QP G+KMISA+QL KGL+ DEPTFMAIP+    NS+E VP+EI
Sbjct: 549 CLVITGPTPSVVQTDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLNSSENSRETVPKEI 608

Query: 121 QQVL----DVSVD----------------------------------------------- 180
            +VL    DV  D                                               
Sbjct: 609 VRVLEKYRDVMPDSLPKYLPPRRMIDHEINWCQGQNRLRRMLIVWRLRNLRSGYYQVRIS 668

Query: 181 ------------------------------------------------------------ 221
                                                                       
Sbjct: 669 EGDEPKTTCVTRYGAFEFLVMPFGLTNASATFCTLMNQVFHEYLDKFVVVYLDDIVVYST 728

[Close]

BLAST of Moc04g11400 vs. NCBI nr
Match: TYK08407.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 201.8 bits (512), Expect = 6.1e-48
Identity = 149/464 (32.11%), Postives = 182/464 (39.22%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MKA+NSAALPI+G+ KR  ++LG WS  VDF++V++DDFDVVL MEFLLE++VI MPLAK
Sbjct: 30  MKAVNSAALPIIGLVKRTTIRLGGWSGLVDFVVVKIDDFDVVLGMEFLLEHQVILMPLAK 89

Query: 61  CLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREI 120
           CLV TGS  +VV   ++QP G+KMISA+QL KGL+ DEPTFMAIP+    NS E VP+EI
Sbjct: 90  CLVITGSTPSVVQVDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEI 149

Query: 121 QQVL----DVSVD----------------------------------------------- 180
            +VL    DV  D                                               
Sbjct: 150 MRVLEKYRDVMPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDEL 209

Query: 181 ------------------------------------------------------------ 227
                                                                       
Sbjct: 210 LNAGFIRLAKAPYGALVLFQKKKDGNLRSGYYQVRIAEGDEPKTTCVTRYGAFEFLVMPF 269

[Close]

BLAST of Moc04g11400 vs. NCBI nr
Match: KAA0035531.1 (uncharacterized protein E6C27_scaffold285G001970 [Cucumis melo var. makuwa])

HSP 1 Score: 201.8 bits (512), Expect = 6.1e-48
Identity = 150/476 (31.51%), Postives = 184/476 (38.66%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MKA+NSAALPI+G+ KR +++LG WS  VDF++V++DDFDVVL MEFLLE++VIPMPLAK
Sbjct: 70  MKAVNSAALPIIGLVKRTMIRLGGWSGPVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAK 129

Query: 61  CLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREI 120
           CLV TG   +VV T ++QP G+KMISA+QL KGL+ DEPTFMAIP+    NS E VP+EI
Sbjct: 130 CLVITGPTPSVVQTDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEI 189

Query: 121 QQVL----DVSVD----------------------------------------------- 180
            +VL    DV  D                                               
Sbjct: 190 MRVLEKYRDVMPDSLPKSLPPRRTIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDEL 249

Query: 181 ------------------------------------------------------------ 227
                                                                       
Sbjct: 250 LNAGFIGPQKLLRNKYPLPIITDLFDRLHGAKYFSKLDLRSGYYQVRIAEGDEPKTTCVT 309

[Close]

BLAST of Moc04g11400 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.3e-13
Identity = 43/105 (40.95%), Postives = 63/105 (60.00%), Query Frame = 0


Query: 128 VDRPLTEILKKNQSWNWTS-ECQPAFESLKKEMMERPMLGITDVARPFEVETDASDFSLG 187
           + +P+T+ LKKN   + T+ E   AF+ LK  + E P+L + D  + F + TDASD +LG
Sbjct: 462 IAKPMTKCLKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALG 521

Query: 188 GVLLQDNHPIAYESRKLNDAEKRYAASGNDMLALV-------HYL 225
            VL QD HP++Y SR LN+ E  Y+    ++LA+V       HYL
Sbjct: 522 AVLSQDGHPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYL 566

[Close]

BLAST of Moc04g11400 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.8e-11
Identity = 35/100 (35.00%), Postives = 59/100 (59.00%), Query Frame = 0


Query: 131 PLTEILKKNQSWNWTSECQPAFESLKKEMMERPMLGITDVARPFEVETDASDFSLGGVLL 190
           PL  +LKK+  W WT     A E++K+ ++  P+L   D ++   +ETDASD ++G VL 
Sbjct: 665 PLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLS 724

Query: 191 QDN-----HPIAYESRKLNDAEKRYAASGNDMLALVHYLR 226
           Q +     +P+ Y S K++ A+  Y+ S  +MLA++  L+
Sbjct: 725 QKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLK 764

[Close]

BLAST of Moc04g11400 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.8e-11
Identity = 35/100 (35.00%), Postives = 59/100 (59.00%), Query Frame = 0


Query: 131 PLTEILKKNQSWNWTSECQPAFESLKKEMMERPMLGITDVARPFEVETDASDFSLGGVLL 190
           PL  +LKK+  W WT     A E++K+ ++  P+L   D ++   +ETDASD ++G VL 
Sbjct: 665 PLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLS 724

Query: 191 QDN-----HPIAYESRKLNDAEKRYAASGNDMLALVHYLR 226
           Q +     +P+ Y S K++ A+  Y+ S  +MLA++  L+
Sbjct: 725 QKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLK 764

[Close]

BLAST of Moc04g11400 vs. ExPASy Swiss-Prot
Match: P0CT34 (Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-1 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.8e-11
Identity = 35/100 (35.00%), Postives = 59/100 (59.00%), Query Frame = 0


Query: 131 PLTEILKKNQSWNWTSECQPAFESLKKEMMERPMLGITDVARPFEVETDASDFSLGGVLL 190
           PL  +LKK+  W WT     A E++K+ ++  P+L   D ++   +ETDASD ++G VL 
Sbjct: 665 PLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLS 724

Query: 191 QDN-----HPIAYESRKLNDAEKRYAASGNDMLALVHYLR 226
           Q +     +P+ Y S K++ A+  Y+ S  +MLA++  L+
Sbjct: 725 QKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLK 764

[Close]

BLAST of Moc04g11400 vs. ExPASy Swiss-Prot
Match: P0CT35 (Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-2 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.8e-11
Identity = 35/100 (35.00%), Postives = 59/100 (59.00%), Query Frame = 0


Query: 131 PLTEILKKNQSWNWTSECQPAFESLKKEMMERPMLGITDVARPFEVETDASDFSLGGVLL 190
           PL  +LKK+  W WT     A E++K+ ++  P+L   D ++   +ETDASD ++G VL 
Sbjct: 665 PLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLS 724

Query: 191 QDN-----HPIAYESRKLNDAEKRYAASGNDMLALVHYLR 226
           Q +     +P+ Y S K++ A+  Y+ S  +MLA++  L+
Sbjct: 725 QKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLK 764

[Close]

BLAST of Moc04g11400 vs. ExPASy TrEMBL
Match: A0A6J1DLQ6 (uncharacterized protein LOC111022320 OS=Momordica charantia OX=3673 GN=LOC111022320 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 3.6e-62
Identity = 176/463 (38.01%), Postives = 195/463 (42.12%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MKA+NSAALPIMGVAKRV VKLGTWS  VDF+IV++DDFDVVL ++FLLE+KVIPMPLAK
Sbjct: 470 MKAVNSAALPIMGVAKRVSVKLGTWSGLVDFVIVRMDDFDVVLGIKFLLEHKVIPMPLAK 529

Query: 61  CLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREI 120
           CLV T SD  VV TSIKQPSGVKMISALQL KG+A DEPTFMAIPV E RNS+E VPREI
Sbjct: 530 CLVVTSSDPIVVQTSIKQPSGVKMISALQLKKGIAQDEPTFMAIPVAEGRNSEELVPREI 589

Query: 121 QQVLDVSVD--------------------------------------------------- 180
           Q+VL+   D                                                   
Sbjct: 590 QRVLEAYADVMPDNLPKNLPPRPGIDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDEL 649

Query: 181 ------------------------------------------------------------ 227
                                                                       
Sbjct: 650 LNAGFIRPAKAPYGAPVLFQKKKDXSLRLCIDYRALNKLXVRNKXPLPIITDLFDQLHGA 709

[Close]

BLAST of Moc04g11400 vs. ExPASy TrEMBL
Match: A0A5A7TF05 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G001550 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 1.3e-51
Identity = 142/406 (34.98%), Postives = 180/406 (44.33%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MK +NSAALPI+G+ KR +++LG WS  VDF++V++DDFDVVL MEFLLE++VIPMPLAK
Sbjct: 375 MKVVNSAALPIIGLVKRTMIRLGGWSGLVDFVVVKIDDFDVVLAMEFLLEHQVIPMPLAK 434

Query: 61  CLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREI 120
           CLV TGS  + V T ++QP G+KMISA +L KGL+ DEPTFMAIP+    NS E VP+EI
Sbjct: 435 CLVITGSTPSFVQTDLRQPDGLKMISARKLKKGLSQDEPTFMAIPLNSSENSGETVPKEI 494

Query: 121 QQVL----DVSVD----------------------------------------------- 180
            +VL    DV  D                                               
Sbjct: 495 MRVLEKYRDVIPDSLPKSLPPRRMIDHEIKLVPRAKPPVKNSYRFIRPTKAPYGAPVLFQ 554

Query: 181 ------------------------------------------------------------ 227
                                                                       
Sbjct: 555 KKKDGSLRLCIDYRVLNKLTVRNKYPLPIITNLFDRLHEAKYFSKLDLRSGYYQVRIAEG 614

[Close]

BLAST of Moc04g11400 vs. ExPASy TrEMBL
Match: A0A5D3CW71 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00870 PE=4 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.6e-49
Identity = 144/421 (34.20%), Postives = 177/421 (42.04%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MKA+NSAALPI+G+ KR +++LG WS  VDF++V++DDFDVVL MEFLLE++VIPMPLAK
Sbjct: 489 MKAVNSAALPIIGLVKRTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHRVIPMPLAK 548

Query: 61  CLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREI 120
           CLV TG   +VV T ++QP G+KMISA+QL KGL+ DEPTFMAIP+    NS+E VP+EI
Sbjct: 549 CLVITGPTPSVVQTDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLNSSENSRETVPKEI 608

Query: 121 QQVL----DVSVD----------------------------------------------- 180
            +VL    DV  D                                               
Sbjct: 609 VRVLEKYRDVMPDSLPKYLPPRRMIDHEINWCQGQNRLRRMLIVWRLRNLRSGYYQVRIS 668

Query: 181 ------------------------------------------------------------ 221
                                                                       
Sbjct: 669 EGDEPKTTCVTRYGAFEFLVMPFGLTNASATFCTLMNQVFHEYLDKFVVVYLDDIVVYST 728

[Close]

BLAST of Moc04g11400 vs. ExPASy TrEMBL
Match: A0A803LKS9 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.0e-48
Identity = 102/226 (45.13%), Postives = 151/226 (66.81%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MKA+NSAA P+ GVA+ +  KLG WS  +DF +  +DDF++VL M+FL   K +PMP  +
Sbjct: 44  MKAVNSAAKPVHGVARNIPTKLGDWSRNLDFAVATMDDFNLVLGMDFLRASKAVPMPHLR 103

Query: 61  CLVETGSDSTVVPT-SIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPRE 120
            ++  G  S ++ T  +++ S   ++SA+QL KGL  +EPTF+A   V++  +   +P  
Sbjct: 104 SVLVAGQQSCLLKTCKMRKGSKGPLLSAMQLKKGLKRNEPTFLATLSVKEDEAPHEIPAA 163

Query: 121 IQQVLDVSVDRPLTEILKKNQSWNWTSECQPAFESLKKEMMERPMLGITDVARPFEVETD 180
           + ++L    D    E+ KK++ W W+ + Q AF+ LK  +M  P+L + +  +PFEVETD
Sbjct: 164 LDKILTEFEDLLPKELPKKDRRWEWSEKQQKAFQELKDSVMREPVLALPNPNKPFEVETD 223

Query: 181 ASDFSLGGVLLQDNHPIAYESRKLNDAEKRYAASGNDMLALVHYLR 226
           ASDF+LGGVLLQD HPIA+ESRKLN AE++YA    ++LA+VH LR
Sbjct: 224 ASDFALGGVLLQDGHPIAFESRKLNSAERKYATQEKELLAIVHCLR 269

[Close]

BLAST of Moc04g11400 vs. ExPASy TrEMBL
Match: A0A5D3C8W7 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00190 PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 3.0e-48
Identity = 149/464 (32.11%), Postives = 182/464 (39.22%), Query Frame = 0


Query: 1   MKAINSAALPIMGVAKRVLVKLGTWSEQVDFMIVQVDDFDVVLEMEFLLEYKVIPMPLAK 60
           MKA+NSAALPI+G+ KR  ++LG WS  VDF++V++DDFDVVL MEFLLE++VI MPLAK
Sbjct: 30  MKAVNSAALPIIGLVKRTTIRLGGWSGLVDFVVVKIDDFDVVLGMEFLLEHQVILMPLAK 89

Query: 61  CLVETGSDSTVVPTSIKQPSGVKMISALQLNKGLANDEPTFMAIPVVEDRNSKEPVPREI 120
           CLV TGS  +VV   ++QP G+KMISA+QL KGL+ DEPTFMAIP+    NS E VP+EI
Sbjct: 90  CLVITGSTPSVVQVDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEI 149

Query: 121 QQVL----DVSVD----------------------------------------------- 180
            +VL    DV  D                                               
Sbjct: 150 MRVLEKYRDVMPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDEL 209

Query: 181 ------------------------------------------------------------ 227
                                                                       
Sbjct: 210 LNAGFIRLAKAPYGALVLFQKKKDGNLRSGYYQVRIAEGDEPKTTCVTRYGAFEFLVMPF 269

The following BLAST results are available for this feature:

BLAST of Moc04g11400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155185.1	7.5e-62	38.01	uncharacterized protein LOC111022320 [Momordica charantia]	[more]
KAA0041498.1	2.7e-51	34.98	uncharacterized protein E6C27_scaffold6G00890 [Cucumis melo var. makuwa] >TYK243...	[more]
KAA0042379.1	3.3e-49	34.20	reverse transcriptase [Cucumis melo var. makuwa] >TYK15498.1 reverse transcripta...	[more]
TYK08407.1	6.1e-48	32.11	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0035531.1	6.1e-48	31.51	uncharacterized protein E6C27_scaffold285G001970 [Cucumis melo var. makuwa]	[more]

BLAST of Moc04g11400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P04323	1.3e-13	40.95	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
Q9UR07	2.8e-11	35.00	Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]
P0CT41	2.8e-11	35.00	Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]
P0CT34	2.8e-11	35.00	Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]
P0CT35	2.8e-11	35.00	Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]

BLAST of Moc04g11400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLQ6	3.6e-62	38.01	uncharacterized protein LOC111022320 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A5A7TF05	1.3e-51	34.98	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5D3CW71	1.6e-49	34.20	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold47...	[more]
A0A803LKS9	1.0e-48	45.13	Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1	[more]
A0A5D3C8W7	3.0e-48	32.11	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold65...	[more]

BLAST of Moc04g11400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 120..182 e-value: 1.5E-7 score: 33.3
IPR041577	Reverse transcriptase/retrotransposon-derived protein, RNase H-like domain	PFAM	PF17919	RT_RNaseH_2	coord: 144..225 e-value: 1.6E-23 score: 82.6
None	No IPR available	PANTHER	PTHR24559:SF324	TRANSPOSON TY3-I GAG-POL POLYPROTEIN-LIKE PROTEIN	coord: 127..221
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 127..221
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 127..225

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g11400.1	Moc04g11400.1	mRNA