Moc04g11260 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g11260
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRibonuclease H
Locationchr4: 8478001 .. 8480967 (-)
RNA-Seq ExpressionMoc04g11260
SyntenyMoc04g11260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGGGTGGGGCCCCTTGTTCAAGTCCCGCCCCTTGTTCAAGTCCCGGAGTCAGCATTTAAGGGAACACTCATCCACTCCCCTAAAGTCGGGGAGGAGTGAATTCCATCTTGTGAAGTTATGTTCCCAGCTCCCCACTCGGTCTCGTCCCCAAAATGGTAGGAATGTTGAGTCGGCGACTCGGGCTACTCTCACCCATACAGATCAAAGGACGAGCCCTCACGGGCAGGAGTCCATAACTCACTCAGGATTGAGAATGAGTTGTCTGGTCATCCTAAGAAATAGCAACCTATTAGTTAACGGTGTTACATCTAAAGATTGCCTATTTCGTGGTCCGGTCTTATACAAACTCATTGTATAGGATACCCCCACTCGCATGTCTCCACACGAACGACCTGGATCGAGTCGTCTGTAACCTTTACAGAGTGGGCCGTATCCATAGTGTTGCCAGGATAAGGTACCCAACCTTATCCATATACTATAGACCCTTTAGGTTATTCACGGGACATTGTCCATTCATATATCAGGGGTATATTAAATATATACCCCGTATATGTACTGTCCCGATTACATCATAAATAACCTTGGATGTTTAGTTTATTGGATTTTGGACAATGCAAAGCTATGATTCAACAACAAATTTATTGAATAAAACTTTATTAAATAGACATTTGTTTTTACAAAAATACAAACTACGAGTTTAGGGCAACACACCCAACATTGCATTCTACATACGCCTTCTCTAGGCATTCTTGTACACATCCTTGGGGTGTGATAGGTGATGCACCATCCTCTTTGGTCGCATCACGGCACTTCTCTCTCGGCACTCAACATGGTCCGACGGACGACACATGAGTGTCGCCACCACTTTTCCACTATGTGGGCGTCACAACCTACTCTCTACTAGTATGGTTGTGACACCTGGTGCTTTTTTGTGGTCAACGTCTTCAAAACGCGCGTTTCACTTAATGAAGGGCAAATGAGTCATTTCCAACATGTTTGAATCAACCAAAAACCGTGAGTTCAAGTCAAGGAAGGTTTGTTGAGAGAATCTCAACCCATCTCCACCTAATATGGTGATAAGTTCAACCATCTTGAGGGCATATAAAAGGAAGCTACTCCCCCAGTAGCAAAGCATCCATTCTTATCCCGTTTTCATCTTCTTTTCTTCCATTCTTAGTTAGAGAATTCGTTCATAAGCTTTAGCGTTACGTTTTAGTTGTTCGTTTCTTGTATTTTCACTCTTTTACTTATCGTAGTGCCTTTAATTCGAGTTTGTACTCTTGTTCTTCATCTATTCCATTCTTTTAAAGTTCTTAAGCGAGTTTACACTCCCCTCACGCTCGAAAGAGAAGAGGGTTAAGCTAGAACTCCTATGCTGTTATTTTCATTTAAATTCAATGGAAATTATTTCCTTTTATGTTTTTGTGTGTTTTATTTCCTTTAATTATGCACTAAGTTATTTATCGACCTCTCCGTGGAATGCCTTAGTCAAATGATTTAATTTTCGACCATGCTCGGGATTTGACCATTTGGTCATAAGTGCATAAAAGACGATCTTAAACATAGGCCCTCTGACCCATAAATAAAAATGAGTTGATAATTACTTTCTAGGATTGTAATCACACATAGAAATATGATTGTAGGTTACCCTAGCCTCTGTATCAACTAAAAACGATTCGTGAAAGAGTAGAACTTATATTCCCAAGATTTAGCCAAATAGTGGCTGGTGCATTTTTCCGAAAGCATGCATCTTCCATGTTTCCATCAAAACTAAGGTAGGAAGTAGAAATGTTGTGTTTTATTAAATTGTTTGATATTTGAAACCCCAAACAACAACATTTTATCACTATTTTCTAAATAACCACAATTGCATCTTTGAACCATTTCAATTACTTGATTTTGAAAGCACAAGTTCCTGAGGATTCAACCTTGGATTACTTCCGAGTATTACTTGTGACCGTGTACGATTGTACGTATTCCTTATCTTTTAAAGGTAGAGAAAGTCCCAGCAAGTTTTTGGCGCCGTTGTCGGGGACTTAATTTTCAAAATCATTTTCTTTTTAAGTGAACTGTTTCATATTGTGATTGTTCTATACCTCTGTTCCTTCAGATTAATTTGTTGTATCTAACCTTAAAGGGCCAATTTTTTTTTTAGCATAAAGGCTTTCTTACAGAAGTCCCTAAAGAGGTTAAGGGGAGAACAAAGGTCAAGAAAACAAAAGAAAAGCTGAAAAAGAGAAGGAAATGAAAAAAGAAATGGGAGGTTACTTGTACTGAGCCAACAACAAGCAGGAGTGTTTGAAATGAAGTCTCAATTTATGTACAATCTTCCAATGGGAAGTAACATGCCCAAAGCTCCTATTAATCAATTCTGTTGTTTATATTGTGGTGACATTTGTCATTCAATGGAATATTGTCCATCTAATCCTTCATCTTTTTATTGTCTAGGTTCGCTAGGAAACCAACAAGAATTCACCCCTTATTCAAATCCATACAACCAAGATTGGTGGGCTCATTCAAATTTATTTTGGGGAGAGCGTCAACATGAAAGTATCCATCCATATGAGCATGTATTCCCATCATAATTCCCAACACAGCCTCAGCAAGAGTACAATCAACCATGGATACCAGAGCAATATAATCTTTGAAAAATTCTAGCCTTGAAGAGATGATGATAGATTTTATGGCAAGAAATGATGAAGCAGTACACAACCAAGATTTTACCGTTGCTTTTGATCATATTCTGCGAGAAAACTATAAAATCATCGATGCACTAGCAACATTATCTTCCACGTTTATTGTAGAGCTAAAGGAGGAAGTAGAACCAATCCAGAGTGAAAAGCGAGAGGTTCCTGCATATTGTCTTTATATTGAAGAAGAAGTGGATGAAAAACCATGGTATTTTGACATTAAACAGTACATTAGGGAATATCCTGAAGGAATCTCAGAAAATGATAAGTGA

mRNA sequence

ATGAGAGGGTGGGGCCCCTTGTTCAAGTCCCGCCCCTTGTTCAAGTCCCGGAGTCAGCATTTAAGGGAACACTCATCCACTCCCCTAAAGTCGGGGAGGAGTGAATTCCATCTTGTGAAGTTATGTTCCCAGCTCCCCACTCGGTCTCGTCCCCAAAATGGTAGGAATGTTGAGTCGGCGACTCGGGCTACTCTCACCCATACAGATCAAAGGACGAGCCCTCACGGGCAGGAGTCCATAACTCACTCAGGATTGAGAATGAGTTGTCTGGTCATCCTAAGAAATAGCAACCTATTAGTTAACGGTGTTACATCTAAAGATTGCCTATTTCGTGGTTCGCTAGGAAACCAACAAGAATTCACCCCTTATTCAAATCCATACAACCAAGATTGCCTTGAAGAGATGATGATAGATTTTATGGCAAGAAATGATGAAGCAGTACACAACCAAGATTTTACCGTTGCTTTTGATCATATTCTGCGAGAAAACTATAAAATCATCGATGCACTAGCAACATTATCTTCCACGTTTATTGTAGAGCTAAAGGAGGAAGTAGAACCAATCCAGAGTGAAAAGCGAGAGGTTCCTGCATATTGTCTTTATATTGAAGAAGAAGTGGATGAAAAACCATGGTATTTTGACATTAAACAGTACATTAGGGAATATCCTGAAGGAATCTCAGAAAATGATAAGTGA

Coding sequence (CDS)

ATGAGAGGGTGGGGCCCCTTGTTCAAGTCCCGCCCCTTGTTCAAGTCCCGGAGTCAGCATTTAAGGGAACACTCATCCACTCCCCTAAAGTCGGGGAGGAGTGAATTCCATCTTGTGAAGTTATGTTCCCAGCTCCCCACTCGGTCTCGTCCCCAAAATGGTAGGAATGTTGAGTCGGCGACTCGGGCTACTCTCACCCATACAGATCAAAGGACGAGCCCTCACGGGCAGGAGTCCATAACTCACTCAGGATTGAGAATGAGTTGTCTGGTCATCCTAAGAAATAGCAACCTATTAGTTAACGGTGTTACATCTAAAGATTGCCTATTTCGTGGTTCGCTAGGAAACCAACAAGAATTCACCCCTTATTCAAATCCATACAACCAAGATTGCCTTGAAGAGATGATGATAGATTTTATGGCAAGAAATGATGAAGCAGTACACAACCAAGATTTTACCGTTGCTTTTGATCATATTCTGCGAGAAAACTATAAAATCATCGATGCACTAGCAACATTATCTTCCACGTTTATTGTAGAGCTAAAGGAGGAAGTAGAACCAATCCAGAGTGAAAAGCGAGAGGTTCCTGCATATTGTCTTTATATTGAAGAAGAAGTGGATGAAAAACCATGGTATTTTGACATTAAACAGTACATTAGGGAATATCCTGAAGGAATCTCAGAAAATGATAAGTGA

Protein sequence

MRGWGPLFKSRPLFKSRSQHLREHSSTPLKSGRSEFHLVKLCSQLPTRSRPQNGRNVESATRATLTHTDQRTSPHGQESITHSGLRMSCLVILRNSNLLVNGVTSKDCLFRGSLGNQQEFTPYSNPYNQDCLEEMMIDFMARNDEAVHNQDFTVAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEKREVPAYCLYIEEEVDEKPWYFDIKQYIREYPEGISENDK
Homology
[Close]
BLAST of Moc04g11260 vs. NCBI nr
Match: TYK00092.1 (protein NYNRIN-like [Cucumis melo var. makuwa])

HSP 1 Score: 92.4 bits (228), Expect = 5.4e-15
Identity = 45/81 (55.56%), Postives = 58/81 (71.60%), Query Frame = 0 

Query: 153 TVAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEKREVPAYCLYIEEEVDEKPWY 212
           ++ F+H+ RE+ ++ DALATLS+ F V   EEV+PI+ EK E P+YC+ +E E D KPWY
Sbjct: 738 SITFEHVPRESNQVADALATLSAMFNVAYSEEVQPIRMEKYETPSYCMSLEREPDGKPWY 797

Query: 213 FDIKQYI--REYPEGISENDK 232
            DIK YI  REYP G SEN K
Sbjct: 798 HDIKHYITHREYPPGASENSK 818

[Close]
BLAST of Moc04g11260 vs. NCBI nr
Match: XP_027191139.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC101489332 [Cicer arietinum])

HSP 1 Score: 92.4 bits (228), Expect = 5.4e-15
Identity = 48/101 (47.52%), Postives = 67/101 (66.34%), Query Frame = 0 

Query: 133  EEMMIDFMARNDEAVHNQDFTVAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEK 192
            +  +I +     E V N +  + F HI RE  ++ DALATLSS F + + ++V  I+ ++
Sbjct: 1475 DSKLIPYHTHIKELVENFEH-ITFHHIPREENQLADALATLSSMFKISVGQDVSVIKIQQ 1534

Query: 193  REVPAYCLYIEEEVDEKPWYFDIKQYI--REYPEGISENDK 232
            +  PAYCL IEEE+D KPW++DIK Y+  REYP GISENDK
Sbjct: 1535 KNKPAYCLSIEEELDSKPWFYDIKSYVKNREYPSGISENDK 1574

[Close]
BLAST of Moc04g11260 vs. NCBI nr
Match: XP_027188669.1 (uncharacterized protein LOC101504764 [Cicer arietinum])

HSP 1 Score: 91.3 bits (225), Expect = 1.2e-14
Identity = 44/80 (55.00%), Postives = 57/80 (71.25%), Query Frame = 0 

Query: 154 VAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEKREVPAYCLYIEEEVDEKPWYF 213
           + F H+ RE+ ++ DALATLSS F +   +E+  I+ E R+ PAYC  +EEE DEKPWY 
Sbjct: 244 ITFHHVPREDNQLADALATLSSMFQINRNDEIPSIKMESRDYPAYCHVMEEETDEKPWYH 303

Query: 214 DIKQYI--REYPEGISENDK 232
           DIK Y+  REYP GISEN+K
Sbjct: 304 DIKHYLINREYPPGISENEK 323

[Close]
BLAST of Moc04g11260 vs. NCBI nr
Match: XP_022147189.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia])

HSP 1 Score: 90.9 bits (224), Expect = 1.6e-14
Identity = 47/86 (54.65%), Postives = 64/86 (74.42%), Query Frame = 0 

Query: 149  NQDF-TVAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEKREVPAYCLYIEEEVD 208
            +Q+F  ++FD++ REN ++ DALATL+  F +EL E+V PI+  +R+VPA C+ IEEE D
Sbjct: 1787 SQEFDEISFDYLPRENNQVADALATLAVMFNLELNEDVRPIKVGRRDVPASCMSIEEEPD 1846

Query: 209  EKPWYFDIKQYI--REYPEGISENDK 232
             KPW+ DIKQYI  +EYP   SENDK
Sbjct: 1847 GKPWFHDIKQYIKSKEYPPNASENDK 1872

[Close]
BLAST of Moc04g11260 vs. NCBI nr
Match: XP_027913807.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC114173538 [Vigna unguiculata])

HSP 1 Score: 90.9 bits (224), Expect = 1.6e-14
Identity = 47/81 (58.02%), Postives = 58/81 (71.60%), Query Frame = 0 

Query: 153  TVAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEKREVPAYCLYIEEEVDEKPWY 212
            ++ F HI RE+ +++DALATLSS F V   E +  IQ +  E PAYC +IEEE D KPWY
Sbjct: 1814 SIEFQHIPREDNQLVDALATLSSMFEVNQDEVLPMIQMKSHEEPAYCHFIEEESDGKPWY 1873

Query: 213  FDIKQYI--REYPEGISENDK 232
            FDIK+Y+  REYPE  SENDK
Sbjct: 1874 FDIKRYLKNREYPEKASENDK 1894

[Close]
BLAST of Moc04g11260 vs. ExPASy TrEMBL
Match: A0A5D3BMZ2 (Protein NYNRIN-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold596G00450 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.6e-15
Identity = 45/81 (55.56%), Postives = 58/81 (71.60%), Query Frame = 0 

Query: 153 TVAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEKREVPAYCLYIEEEVDEKPWY 212
           ++ F+H+ RE+ ++ DALATLS+ F V   EEV+PI+ EK E P+YC+ +E E D KPWY
Sbjct: 738 SITFEHVPRESNQVADALATLSAMFNVAYSEEVQPIRMEKYETPSYCMSLEREPDGKPWY 797

Query: 213 FDIKQYI--REYPEGISENDK 232
            DIK YI  REYP G SEN K
Sbjct: 798 HDIKHYITHREYPPGASENSK 818

[Close]
BLAST of Moc04g11260 vs. ExPASy TrEMBL
Match: A0A3Q7XC48 (Reverse transcriptase OS=Cicer arietinum OX=3827 GN=LOC101489332 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.6e-15
Identity = 48/101 (47.52%), Postives = 67/101 (66.34%), Query Frame = 0 

Query: 133  EEMMIDFMARNDEAVHNQDFTVAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEK 192
            +  +I +     E V N +  + F HI RE  ++ DALATLSS F + + ++V  I+ ++
Sbjct: 1475 DSKLIPYHTHIKELVENFEH-ITFHHIPREENQLADALATLSSMFKISVGQDVSVIKIQQ 1534

Query: 193  REVPAYCLYIEEEVDEKPWYFDIKQYI--REYPEGISENDK 232
            +  PAYCL IEEE+D KPW++DIK Y+  REYP GISENDK
Sbjct: 1535 KNKPAYCLSIEEELDSKPWFYDIKSYVKNREYPSGISENDK 1574

[Close]
BLAST of Moc04g11260 vs. ExPASy TrEMBL
Match: A0A3Q7X4P5 (Ribonuclease H OS=Cicer arietinum OX=3827 GN=LOC101504764 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 5.8e-15
Identity = 44/80 (55.00%), Postives = 57/80 (71.25%), Query Frame = 0 

Query: 154 VAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEKREVPAYCLYIEEEVDEKPWYF 213
           + F H+ RE+ ++ DALATLSS F +   +E+  I+ E R+ PAYC  +EEE DEKPWY 
Sbjct: 244 ITFHHVPREDNQLADALATLSSMFQINRNDEIPSIKMESRDYPAYCHVMEEETDEKPWYH 303

Query: 214 DIKQYI--REYPEGISENDK 232
           DIK Y+  REYP GISEN+K
Sbjct: 304 DIKHYLINREYPPGISENEK 323

[Close]
BLAST of Moc04g11260 vs. ExPASy TrEMBL
Match: A0A6J1D099 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 7.6e-15
Identity = 47/86 (54.65%), Postives = 64/86 (74.42%), Query Frame = 0 

Query: 149  NQDF-TVAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEKREVPAYCLYIEEEVD 208
            +Q+F  ++FD++ REN ++ DALATL+  F +EL E+V PI+  +R+VPA C+ IEEE D
Sbjct: 1787 SQEFDEISFDYLPRENNQVADALATLAVMFNLELNEDVRPIKVGRRDVPASCMSIEEEPD 1846

Query: 209  EKPWYFDIKQYI--REYPEGISENDK 232
             KPW+ DIKQYI  +EYP   SENDK
Sbjct: 1847 GKPWFHDIKQYIKSKEYPPNASENDK 1872

[Close]
BLAST of Moc04g11260 vs. ExPASy TrEMBL
Match: A0A3Q7XX44 (Ribonuclease H OS=Cicer arietinum OX=3827 GN=LOC101492158 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 9.9e-15
Identity = 44/80 (55.00%), Postives = 59/80 (73.75%), Query Frame = 0 

Query: 154  VAFDHILRENYKIIDALATLSSTFIVELKEEVEPIQSEKREVPAYCLYIEEEVDEKPWYF 213
            + F HILRE  ++ DALA LSS F +   ++V  I+ ++R+ PAYCL IEEE+D KPW++
Sbjct: 1788 ITFHHILREENQLADALAILSSMFKITTNQDVPVIKIQQRDKPAYCLXIEEELDGKPWFY 1847

Query: 214  DIKQYI--REYPEGISENDK 232
            DIK Y+  +EYP GISENDK
Sbjct: 1848 DIKSYVKNKEYPLGISENDK 1867

The following BLAST results are available for this feature:
BLAST of Moc04g11260 vs. NCBI nr
Match NameE-valueIdentityDescription
TYK00092.15.4e-1555.56protein NYNRIN-like [Cucumis melo var. makuwa][more]
XP_027191139.15.4e-1547.52LOW QUALITY PROTEIN: uncharacterized protein LOC101489332 [Cicer arietinum][more]
XP_027188669.11.2e-1455.00uncharacterized protein LOC101504764 [Cicer arietinum][more]
XP_022147189.11.6e-1454.65LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia][more]
XP_027913807.11.6e-1458.02LOW QUALITY PROTEIN: uncharacterized protein LOC114173538 [Vigna unguiculata][more]
BLAST of Moc04g11260 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Moc04g11260 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3BMZ22.6e-1555.56Protein NYNRIN-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold596G... [more]
A0A3Q7XC482.6e-1547.52Reverse transcriptase OS=Cicer arietinum OX=3827 GN=LOC101489332 PE=4 SV=1[more]
A0A3Q7X4P55.8e-1555.00Ribonuclease H OS=Cicer arietinum OX=3827 GN=LOC101504764 PE=4 SV=1[more]
A0A6J1D0997.6e-1554.65Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1[more]
A0A3Q7XX449.9e-1555.00Ribonuclease H OS=Cicer arietinum OX=3827 GN=LOC101492158 PE=4 SV=1[more]
BLAST of Moc04g11260 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..80
NoneNo IPR availablePANTHERPTHR46387:SF5DNA POLYMERASE (REVERSE TRANSCRIPTASE), RIBONUCLEASE H, PUTATIVE-RELATEDcoord: 151..231
NoneNo IPR availablePANTHERPTHR46387POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEINcoord: 151..231

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g11260.1Moc04g11260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0140640 catalytic activity, acting on a nucleic acid
molecular_function GO:0003676 nucleic acid binding