Moc04g09940 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g09940
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr4: 7389248 .. 7391975 (-)
RNA-Seq Expression	Moc04g09940
Synteny	Moc04g09940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAAAAGACGACACGGCGCCGAGGCCATCACGGCGTCGAGGCGCCGACCCCACCTTGGCGCCGAGGTGTCTGCTTCTCACCTTGGCCTTCGAGCCAAGGTATAATCGACTTCCTCCGCACAGATCTAACGTCAAAAGGTTAAACACACCTGTTCGGGGCCAGTCCAACATCCACCTCTTCTGTTGATTAACAATCGTTGGGCCTTACAACTCCGAATTAGGGCTCGACTACAAAAGGTTCTGGAACCATCCTCACAGCTATAAATAGCAGTGCAGCCTAACTATGGAAAGGCAAGTAACATACTTGAAACTCAATATTTCTCAACTTCCCTTTGCTAATTTAGGCATCGGAGCCCATGTGATTAAGGTCGCACCGGTTTAGATCGGTTTAGTTTTGCAGGTATCAGTTCCTGTATCATCGAGTTAGGTATGTGCGAGGAGGAAAGTCGGAGGGTCGAAATCTACACATCAATAATTGGCGCCGTCGGTGGGGATAATACATCAAAGCAAGCTTAGTCGAAATGAAATCTCAATCCGAGGCGTGTAGAGTGGAGCAAGACGACATGAATCCTGGTGAGAGTCAGCAGTGTTTCGAAAAGAAGAGCCTGAATGAAGCGGCCGACCACTCAGGTGAGGCGAGACCGAGCCCTGATCATCGACTTAAATCACCTACCCTTAACAGGGGAGAACCGGCTTCGAGCCTGAATGAAGCGACCGACCACTCGGGTGAGGCAAGACCGAGCCCCGGTCATCGACTTAATTCGACCACCCTTGACAAGGGAGAATCGGCTGAAGAGGTAGAATCTGTCCCTCTGACAGTCGAAGATCAACGAGTCAACATTGGAACCAGATTGGGGAATGACGAAAGAGAAAGCCTTAAAAACTTCCTCAGAAGCAACGCCGACGTGTTGGCATGATTACATGAGAACATGCCGGGAATCGATCGAGGAGTCATGGTACATCGACTCAATGTCGACGAGAGCTGTAAGCCAATGAAACGGAAGCGTCGTGCATTCAACCAAGAAAGGAATGAGATCGTAGCTGAGTAGGTAGAGGAGCTACTGAAAGCAGACTTCATTTGAGAAGTACACTATCCCAAGTGGATATCCAACGTAGTGCTAGTAAAAAAGCCAAACGGCGAATGGAGAATGTGCATAAACTTCACAGACCTAAATAAGGCATGTCCAAAGGATTCTTTTCCCTTGCCGCGAATAGATTAACTGGTTGATGCCACTGCAGGACATGAAAGATTGAGTTTCATTTACGCCTACTCCGAATATAATCAAATCAAGATGCATTTACCGGATCAAGAGAAAACCTCTTTTGTCACGAGCCGAGGTTTGTATTGCTACATGGTGATGCCATTCGGATTGAAGAATGCAGGTGCAACCTACCAATGAATGGTGAATCGAATGTTTGCAGCCTTAATCGGCAAAAATATGGAAGTCTATGTAGACGATATGCTGGTAAAGAGTTAAAAGAATAAGCGACACGAGGAGGATTTGGCAGAAGCATTCGACGTTTTGCGAAAGTACAAGATGAAGCTAAATCCTACCAAATGCACGTTTGGAGTGACCTCAGGCAAGTTCCTGGCATTTTTAGTACACCAAAGAGGAATTGAAGCCAATCCAGATAAGATCAAAGCTATTCTGGAGATGGATCCACCTAGAAATTTAAAGCAACTATAAGGACTCAACGGAAGGATAGCAGCACTCAACAGATTCGTCAGCCGTTTGACTGACAAGTGCCTACCATTCTTCAAAATCCTGAGGAAGAAAGGGCAATCTGAATGGACAGAAGAGGGTGAGGCTGCCTTTAAGGAGCTCAAGCAATACCTAGGGACAGGCCCACTCCACTCAAAGCCGATCGTGGGTGATGAACTCTACTTATACTTAGCTATATCCGACACTGCAATGAATTCCGCGTTGATAAAAGAAGAAGATGGACGACAGTCTCCAATCTACTACACAAGCAAAGCAATGGTAGATGCAGAGCTTAGATACCTGTAAATGAAGAAATTGGCGTTAACACTGGTCACTTCGGCTCGGAGACTACGTCCGTATTTCCAAGCTCACAGAATAACTATCCTCACCAACTTTCCCTTACGACGGATTTTGCAAAGGCCAGACACTTCTGGGCGACTGGTAAAGTGGACTGTGGAGCTCAATGTGTACGATATTCACTATAAGCCAATAACTGCAATGAAATGGTAGGCTGTGGCAGACTTCATAGCAAAATTAACACCAACAGAACCTCAAGTATATAGTATCGACCCACAATTGGTTGAGGAAGAGTAACCCTGGACACTCTACGTAGATGAGTCCTAGTCTCTCTAGATAGAGATGGATCCAACAACGAAATCGAGTATGAAGCTCTGTTAGAAGGACTAAAAGTGGCCAGGTACATTAGAGTCAGGAACATACGGATCCTCAGTGATTCAGAACTGATTGTAGCTTAGGTGAAAGATGAGTTCTAAGGCGAAATTCCTTTCTCAGGTAAAAGACCAGCTCGACCAGTTCTCAAAATATGAGATTCGACAAATTCCATGTACTCAAAACGTCAATGTAGATGCGTTAGCTCGACTAGCTGCAACTTATGAAACTGGCTTAGGAAGAACTGTACCAGTCGAGATTCTGCCCGAGCCAAGCATAGAAGCTCAAGAAGCAATGGATATCGACGGGATTCTGCCTACTGAAACTCACTATTTCTCAACTCTCCTTTGCTAA

mRNA sequence

ATGCCAAAAGACGACACGGCGCCGAGGCCATCACGGCGTCGAGGCGCCGACCCCACCTTGGCGCCGAGGTGTCTGCTTCTCACCTTGGCCTTCGAGCCAAGAGTGGAGCAAGACGACATGAATCCTGGTGAGAGTCAGCAGTGTTTCGAAAAGAAGAGCCTGAATGAAGCGGCCGACCACTCAGGTGAGGCGAGACCGAGCCCTGATCATCGACTTAAATCACCTACCCTTAACAGGGGAGAACCGGCTTCGAGCCTGAATGAAGCGACCGACCACTCGGGTGAGGCAAGACCGAGCCCCGGTCATCGACTTAATTCGACCACCCTTGACAAGGGAGAATCGGCTGAAGAGGTAAAAGACCAGCTCGACCAGTTCTCAAAATATGAGATTCGACAAATTCCATGTACTCAAAACGTCAATGTAGATGCGTTAGCTCGACTAGCTGCAACTTATGAAACTGGCTTAGGAAGAACTGTACCAGTCGAGATTCTGCCCGAGCCAAGCATAGAAGCTCAAGAAGCAATGGATATCGACGGGATTCTGCCTACTGAAACTCACTATTTCTCAACTCTCCTTTGCTAA

Coding sequence (CDS)

ATGCCAAAAGACGACACGGCGCCGAGGCCATCACGGCGTCGAGGCGCCGACCCCACCTTGGCGCCGAGGTGTCTGCTTCTCACCTTGGCCTTCGAGCCAAGAGTGGAGCAAGACGACATGAATCCTGGTGAGAGTCAGCAGTGTTTCGAAAAGAAGAGCCTGAATGAAGCGGCCGACCACTCAGGTGAGGCGAGACCGAGCCCTGATCATCGACTTAAATCACCTACCCTTAACAGGGGAGAACCGGCTTCGAGCCTGAATGAAGCGACCGACCACTCGGGTGAGGCAAGACCGAGCCCCGGTCATCGACTTAATTCGACCACCCTTGACAAGGGAGAATCGGCTGAAGAGGTAAAAGACCAGCTCGACCAGTTCTCAAAATATGAGATTCGACAAATTCCATGTACTCAAAACGTCAATGTAGATGCGTTAGCTCGACTAGCTGCAACTTATGAAACTGGCTTAGGAAGAACTGTACCAGTCGAGATTCTGCCCGAGCCAAGCATAGAAGCTCAAGAAGCAATGGATATCGACGGGATTCTGCCTACTGAAACTCACTATTTCTCAACTCTCCTTTGCTAA

Protein sequence

MPKDDTAPRPSRRRGADPTLAPRCLLLTLAFEPRVEQDDMNPGESQQCFEKKSLNEAADHSGEARPSPDHRLKSPTLNRGEPASSLNEATDHSGEARPSPGHRLNSTTLDKGESAEEVKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQEAMDIDGILPTETHYFSTLLC

Homology

[Close]

BLAST of Moc04g09940 vs. NCBI nr
Match: XP_022156571.1 (uncharacterized protein LOC111023448 [Momordica charantia])

HSP 1 Score: 105.1 bits (261), Expect = 6.7e-19
Identity = 55/76 (72.37%), Postives = 61/76 (80.26%), Query Frame = 0


Query: 117 EVKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQEAMD 176
           +V++QLDQFSKYEI QIP  QNV+ DALARLAA YET LGRTVPVEILPEPSIEAQEAMD
Sbjct: 744 QVRNQLDQFSKYEIXQIPRAQNVBADALARLAAXYETDLGRTVPVEILPEPSIEAQEAMD 803

Query: 177 IDGILPTETHYFSTLL 193
           ID     E +  S L+
Sbjct: 804 IDNQGQXEENXKSPLI 819

[Close]

BLAST of Moc04g09940 vs. NCBI nr
Match: XP_022156571.1 (uncharacterized protein LOC111023448 [Momordica charantia])

HSP 1 Score: 101.3 bits (251), Expect = 9.7e-18
Identity = 51/73 (69.86%), Postives = 59/73 (80.82%), Query Frame = 0


Query: 40  MNPGESQQCFEKKSLNEAADHSGEARPSPDHRLKSPTLNRGEPASSLNEATDHSGEARPS 99
           MNP ES +CFE+KSL +AADH  EARPSPD +L   TL+ GEP S LN+ATD SG+ARPS
Sbjct: 1   MNPDESHRCFEEKSLKKAADHLDEARPSPDRQLNPTTLDGGEPVSGLNKATDLSGKARPS 60

Query: 100 PGHRLNSTTLDKG 113
           PG+RLN TTLDKG
Sbjct: 61  PGYRLNPTTLDKG 73

HSP 2 Score: 104.0 bits (258), Expect = 1.5e-18
Identity = 54/76 (71.05%), Postives = 60/76 (78.95%), Query Frame = 0


Query: 117 EVKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQEAMD 176
           +V++QLDQFSKYEIRQIP  QNVN D LARLA  YET LGRTVPVEILP PSIEAQEAMD
Sbjct: 407 QVRNQLDQFSKYEIRQIPHAQNVNADVLARLAVAYETDLGRTVPVEILPGPSIEAQEAMD 466

Query: 177 IDGILPTETHYFSTLL 193
           ID     E ++ S L+
Sbjct: 467 IDNQGQPEENWKSPLI 482

[Close]

BLAST of Moc04g09940 vs. NCBI nr
Match: XP_022154103.1 (uncharacterized protein LOC111021439 [Momordica charantia])

HSP 1 Score: 103.2 bits (256), Expect = 2.5e-18
Identity = 55/76 (72.37%), Postives = 61/76 (80.26%), Query Frame = 0


Query: 117 EVKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQEAMD 176
           +V++QLDQFSKYEIRQIP  QNVN DALARLA TYET LGRTVPVEIL  PSIEAQEAMD
Sbjct: 200 QVRNQLDQFSKYEIRQIPRAQNVNADALARLATTYETDLGRTVPVEILLGPSIEAQEAMD 259

Query: 177 IDGILPTETHYFSTLL 193
           ID     E ++ S L+
Sbjct: 260 IDDQGQPEENWKSLLI 275

[Close]

BLAST of Moc04g09940 vs. NCBI nr
Match: XP_022159204.1 (uncharacterized protein LOC111025623 [Momordica charantia])

HSP 1 Score: 102.4 bits (254), Expect = 4.3e-18
Identity = 53/75 (70.67%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 118 VKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQEAMDI 177
           VKDQL QFSK+EIRQIPCTQNVN DALARLAA Y T LGRT+ VEIL EPSI+AQE MDI
Sbjct: 348 VKDQLGQFSKFEIRQIPCTQNVNADALARLAAAYGTDLGRTISVEILLEPSIKAQEKMDI 407

Query: 178 DGILPTETHYFSTLL 193
           D    +E ++ S L+
Sbjct: 408 DEQRQSEENWMSHLI 422

[Close]

BLAST of Moc04g09940 vs. NCBI nr
Match: XP_022131678.1 (uncharacterized protein LOC111004795 [Momordica charantia])

HSP 1 Score: 97.4 bits (241), Expect = 1.4e-16
Identity = 51/72 (70.83%), Postives = 57/72 (79.17%), Query Frame = 0


Query: 113 ESAEEVKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQ 172
           E    VKDQL  FSKYE  QIP +QNVN +ALARLAAT ETGLGRTVPVEILPEPSI+ Q
Sbjct: 2   EYLARVKDQLSLFSKYEFMQIPRSQNVNANALARLAATCETGLGRTVPVEILPEPSIKVQ 61

Query: 173 EAMDID--GILP 183
           + MD+D  G+LP
Sbjct: 62  DVMDVDLIGLLP 73

[Close]

BLAST of Moc04g09940 vs. ExPASy TrEMBL
Match: A0A6J1DVC6 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 7.2e-19
Identity = 55/76 (72.37%), Postives = 61/76 (80.26%), Query Frame = 0


Query: 117 EVKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQEAMD 176
           +V++QLDQFSKYEI QIP  QNV+ DALARLAA YET LGRTVPVEILPEPSIEAQEAMD
Sbjct: 744 QVRNQLDQFSKYEIXQIPRAQNVDADALARLAAXYETDLGRTVPVEILPEPSIEAQEAMD 803

Query: 177 IDGILPTETHYFSTLL 193
           ID     E +  S L+
Sbjct: 804 IDNQGQXEENXKSPLI 819

[Close]

BLAST of Moc04g09940 vs. ExPASy TrEMBL
Match: A0A6J1DVC6 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 4.7e-18
Identity = 51/73 (69.86%), Postives = 59/73 (80.82%), Query Frame = 0


Query: 40  MNPGESQQCFEKKSLNEAADHSGEARPSPDHRLKSPTLNRGEPASSLNEATDHSGEARPS 99
           MNP ES +CFE+KSL +AADH  EARPSPD +L   TL+ GEP S LN+ATD SG+ARPS
Sbjct: 1   MNPDESHRCFEEKSLKKAADHLDEARPSPDRQLNPTTLDGGEPVSGLNKATDLSGKARPS 60

Query: 100 PGHRLNSTTLDKG 113
           PG+RLN TTLDKG
Sbjct: 61  PGYRLNPTTLDKG 73

HSP 2 Score: 104.0 bits (258), Expect = 7.2e-19
Identity = 54/76 (71.05%), Postives = 60/76 (78.95%), Query Frame = 0


Query: 117 EVKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQEAMD 176
           +V++QLDQFSKYEIRQIP  QNVN D LARLA  YET LGRTVPVEILP PSIEAQEAMD
Sbjct: 407 QVRNQLDQFSKYEIRQIPHAQNVNADVLARLAVAYETDLGRTVPVEILPGPSIEAQEAMD 466

Query: 177 IDGILPTETHYFSTLL 193
           ID     E ++ S L+
Sbjct: 467 IDNQGQPEENWKSPLI 482

[Close]

BLAST of Moc04g09940 vs. ExPASy TrEMBL
Match: A0A6J1DJC7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111021439 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.2e-18
Identity = 55/76 (72.37%), Postives = 61/76 (80.26%), Query Frame = 0


Query: 117 EVKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQEAMD 176
           +V++QLDQFSKYEIRQIP  QNVN DALARLA TYET LGRTVPVEIL  PSIEAQEAMD
Sbjct: 200 QVRNQLDQFSKYEIRQIPRAQNVNADALARLATTYETDLGRTVPVEILLGPSIEAQEAMD 259

Query: 177 IDGILPTETHYFSTLL 193
           ID     E ++ S L+
Sbjct: 260 IDDQGQPEENWKSLLI 275

[Close]

BLAST of Moc04g09940 vs. ExPASy TrEMBL
Match: A0A6J1E1Q5 (uncharacterized protein LOC111025623 OS=Momordica charantia OX=3673 GN=LOC111025623 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 2.1e-18
Identity = 53/75 (70.67%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 118 VKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQEAMDI 177
           VKDQL QFSK+EIRQIPCTQNVN DALARLAA Y T LGRT+ VEIL EPSI+AQE MDI
Sbjct: 348 VKDQLGQFSKFEIRQIPCTQNVNADALARLAAAYGTDLGRTISVEILLEPSIKAQEKMDI 407

Query: 178 DGILPTETHYFSTLL 193
           D    +E ++ S L+
Sbjct: 408 DEQRQSEENWMSHLI 422

[Close]

BLAST of Moc04g09940 vs. ExPASy TrEMBL
Match: A0A6J1BU48 (uncharacterized protein LOC111004795 OS=Momordica charantia OX=3673 GN=LOC111004795 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 6.8e-17
Identity = 51/72 (70.83%), Postives = 57/72 (79.17%), Query Frame = 0


Query: 113 ESAEEVKDQLDQFSKYEIRQIPCTQNVNVDALARLAATYETGLGRTVPVEILPEPSIEAQ 172
           E    VKDQL  FSKYE  QIP +QNVN +ALARLAAT ETGLGRTVPVEILPEPSI+ Q
Sbjct: 2   EYLARVKDQLSLFSKYEFMQIPRSQNVNANALARLAATCETGLGRTVPVEILPEPSIKVQ 61

Query: 173 EAMDID--GILP 183
           + MD+D  G+LP
Sbjct: 62  DVMDVDLIGLLP 73

The following BLAST results are available for this feature:

BLAST of Moc04g09940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156571.1	6.7e-19	72.37	uncharacterized protein LOC111023448 [Momordica charantia]	[more]
XP_022156571.1	9.7e-18	69.86	uncharacterized protein LOC111023448 [Momordica charantia]	[more]
XP_022154103.1	2.5e-18	72.37	uncharacterized protein LOC111021439 [Momordica charantia]	[more]
XP_022159204.1	4.3e-18	70.67	uncharacterized protein LOC111025623 [Momordica charantia]	[more]
XP_022131678.1	1.4e-16	70.83	uncharacterized protein LOC111004795 [Momordica charantia]	[more]

BLAST of Moc04g09940 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc04g09940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DVC6	7.2e-19	72.37	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1	[more]
A0A6J1DVC6	4.7e-18	69.86	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1	[more]
A0A6J1DJC7	1.2e-18	72.37	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111021439 PE=4 SV=1	[more]
A0A6J1E1Q5	2.1e-18	70.67	uncharacterized protein LOC111025623 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1BU48	6.8e-17	70.83	uncharacterized protein LOC111004795 OS=Momordica charantia OX=3673 GN=LOC111004...	[more]

BLAST of Moc04g09940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 193..193
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..76
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 35..115
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 78..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g09940.1	Moc04g09940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity