Moc04g08820 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g08820
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	magnesium-dependent phosphatase 1
Location	chr4: 6389487 .. 6391560 (-)
RNA-Seq Expression	Moc04g08820
Synteny	Moc04g08820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGACGAGAATGTCAAGGCGGAGGCTCTGCAGATTATAGGAATGTTCCAAGTTCTTCCAAAATTGGTGGTCTTCGACCTCGATTACACTCTCTGGCCATTCTATTGGTAATTTATCATTCTCTTCAAATTCACTTCCTGTCTGTTGACTCAGAAAAACGCATGTCCATAGATTCTTTCCTAGAACTGGTTAATCCGTTTGATTTTCGAGCCGATGAAGTTCTGTTATACTTCCCCTTCGAATTCGCAGTAAGGGAATTTGCGAAATAATTCGGTTACTGTGGTCTATTTTGACCTTTGTTTTAGTTGTTTTTCATCCTGTTCGCCCTATAATTAATTTGTTGACTCGAACGAGAAGATTGTTCGAAGAAATGAAACAGGACTACACCATGTTACAAATTAAATTCATTCACTACGTTTCGTGTTATTCTCCATCGAGATTGTTAATTAGGTTATTTTCCTATCTTATTAACTTATGTTCGTGGGACTTGAGCGAATTGGAATTAAAATGGAGCCGTGTATGGATACTAAGATCAATCTTTTCTTTTTCTTCTTTGTCAACGGCAGCCTTATGGCGTTCGTGTAGGCATTCGCGCCATAATCTCTACTGCGATTATCGTTTCTTAATCTGTAGTTATTAAGAAGTGTCAACTCTACAGCCCTAATCCTGAAGGAGGTTTTGCCCAACCTAAAGCCCGCTTTGAGACACAAAAAGGGTTATAAATTATTAAGTCCTTGTCTCTAAGTAGCAGCTCAGTTGGCAAGGGCTTTGGACCTCTTCATCATACTGGCTTAGAGGCTGAAGTTCGAACCTTTTTGGTGAGCTTAATGCCAAAAATCCTTGTCTCTCCGGGTCTGGGCCTTGGGGCGCGGGTGCTCCTGGGTATAGGCCATAGGAGAGCAAAGCTCCGAGTCCCCGTTATCATATAAAAAAAAAAAAAAATATATATATATATATATATTAACTTGTTAACTTAGGCTTGAACCCAAATAAGTTCTTACCATCAAGTCACCTATCGGGGTTGGTTGAGGATTAGTTATCAACCGTATAGACTTGCTTTACATGGTTGTTTCTGTAATTGTTAAGACGTATATCGAAGTTGACCCTCTTCGTGTTCTTTTTACAGTGAATGCCGCTCCAAACGTGATATGCCATCTCTGTATCCTCATGCCACAGGCATATTATATGCACTTAAAGACAAGGGAATTGACCTTGCAATTGCTTCTAGATCACCAACATCTGATATTGCCAAGACGTTTATTGACAAATTGGGCATCAAGTCCATGTTCGTAGCTCAGGTATTTCTTGTTCAGTTTCCAGCTTTGAAATTGTTGTATATTTCTTCTGGTTCCTCGTTGAATGACGAAAAATGATGTTTTTCCAGCAATATGATCATGCGAGGAGGAATGAACTTTGATATATTGTAGCTAAAAGAATAAATTAGTAAGGAGGAAATGCAATTTCTCTCCTACTAGCACTAAGTTGTACATGGTTTATTCAACAACCTTCCTATACAAGGAACATAGTCTTGGGTGCACTTCCTTCCATTTTTTTCTTAAATAATCGGCTTATAGTGGGATGTCTTCGTATTAATTCCATCCTGTTTTTCATGCTCAACCATCTTTACCAGGAGATTTTTTCCAGTTGGACGCACAAAACAGATCATTTTCAGAGAATTCATTCGAGGACAAATGTACCATTCAAATGTATGCTCTTCTTTGATGACGAGGATCGGAACATTGAAACTGTAAGACTCATTTTTACCACAGCTTATTTTCTATGTTTGTTGCATGTATCTAAGACGGTGCTTTTTGAAGATTGTATTGGCATAAATCCTTTTTTAAGGATGTCTTTTCTCTTTATTGCCATCTTAACAAAGATTTATGATGGTAATTGGTGACTACAGGTTTCAAAAATGGGAGTAACTAGTATTCTAGTTGACAACGGAGTAAATCTTGGCGCGCTGAGGCAAGGACTCTCGGAATACTCCCAGAATGTGAATAAAATTGAGAAGAACAAGCAGAAATGGAAAAAGTTCACTCAGAACTCAACTTCCTCGGAGAAAAAATGA

mRNA sequence

ATGGCCGACGAGAATGTCAAGGCGGAGGCTCTGCAGATTATAGGAATGTTCCAAGTTCTTCCAAAATTGGTGGTCTTCGACCTCGATTACACTCTCTGGCCATTCTATTGTGAATGCCGCTCCAAACGTGATATGCCATCTCTGTATCCTCATGCCACAGGCATATTATATGCACTTAAAGACAAGGGAATTGACCTTGCAATTGCTTCTAGATCACCAACATCTGATATTGCCAAGACGTTTATTGACAAATTGGGCATCAAGTCCATGTTCGTAGCTCAGGAGATTTTTTCCAGTTGGACGCACAAAACAGATCATTTTCAGAGAATTCATTCGAGGACAAATGTACCATTCAAATGTATGCTCTTCTTTGATGACGAGGATCGGAACATTGAAACTGTTTCAAAAATGGGAGTAACTAGTATTCTAGTTGACAACGGAGTAAATCTTGGCGCGCTGAGGCAAGGACTCTCGGAATACTCCCAGAATGTGAATAAAATTGAGAAGAACAAGCAGAAATGGAAAAAGTTCACTCAGAACTCAACTTCCTCGGAGAAAAAATGA

Coding sequence (CDS)

ATGGCCGACGAGAATGTCAAGGCGGAGGCTCTGCAGATTATAGGAATGTTCCAAGTTCTTCCAAAATTGGTGGTCTTCGACCTCGATTACACTCTCTGGCCATTCTATTGTGAATGCCGCTCCAAACGTGATATGCCATCTCTGTATCCTCATGCCACAGGCATATTATATGCACTTAAAGACAAGGGAATTGACCTTGCAATTGCTTCTAGATCACCAACATCTGATATTGCCAAGACGTTTATTGACAAATTGGGCATCAAGTCCATGTTCGTAGCTCAGGAGATTTTTTCCAGTTGGACGCACAAAACAGATCATTTTCAGAGAATTCATTCGAGGACAAATGTACCATTCAAATGTATGCTCTTCTTTGATGACGAGGATCGGAACATTGAAACTGTTTCAAAAATGGGAGTAACTAGTATTCTAGTTGACAACGGAGTAAATCTTGGCGCGCTGAGGCAAGGACTCTCGGAATACTCCCAGAATGTGAATAAAATTGAGAAGAACAAGCAGAAATGGAAAAAGTTCACTCAGAACTCAACTTCCTCGGAGAAAAAATGA

Protein sequence

MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALKDKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKCMLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQNSTSSEKK

Homology

[Close]

BLAST of Moc04g08820 vs. NCBI nr
Match: XP_022136381.1 (magnesium-dependent phosphatase 1 [Momordica charantia])

HSP 1 Score: 375.6 bits (963), Expect = 2.6e-100
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK
Sbjct: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180

Query: 181 STSSEKK 188
           STSSEKK
Sbjct: 181 STSSEKK 187

[Close]

BLAST of Moc04g08820 vs. NCBI nr
Match: XP_022988962.1 (magnesium-dependent phosphatase 1-like [Cucurbita maxima])

HSP 1 Score: 348.6 bits (893), Expect = 3.4e-92
Identity = 172/186 (92.47%), Postives = 179/186 (96.24%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKR+MPSLYPHA GILYALK
Sbjct: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKREMPSLYPHAKGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTF+DKLGI SMFVAQEIFSSWTHKTDHFQRIHSRT VPF  
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFVDKLGINSMFVAQEIFSSWTHKTDHFQRIHSRTGVPFNS 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILV NGVNLGALRQGL++Y+QNVNKIEKNKQKW K+T+N
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVGNGVNLGALRQGLTKYTQNVNKIEKNKQKWTKYTKN 180

Query: 181 STSSEK 187
           STSSEK
Sbjct: 181 STSSEK 186

[Close]

BLAST of Moc04g08820 vs. NCBI nr
Match: XP_022927945.1 (magnesium-dependent phosphatase 1 [Cucurbita moschata] >KAG6588747.1 Magnesium-dependent phosphatase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 346.7 bits (888), Expect = 1.3e-91
Identity = 171/186 (91.94%), Postives = 179/186 (96.24%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           MADE+VKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKR+MPSLYPHA GILYALK
Sbjct: 1   MADESVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKREMPSLYPHAKGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTF+DKLGI SMFVAQEIFSSWTHKTDHFQRIHSRT VPF  
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFVDKLGINSMFVAQEIFSSWTHKTDHFQRIHSRTGVPFNS 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILV NGVNLGALRQGL++Y+QNVNKIEKNKQKW K+T+N
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVGNGVNLGALRQGLTKYTQNVNKIEKNKQKWTKYTKN 180

Query: 181 STSSEK 187
           STSSEK
Sbjct: 181 STSSEK 186

[Close]

BLAST of Moc04g08820 vs. NCBI nr
Match: KAG7022531.1 (Beta-ureidopropionase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 346.7 bits (888), Expect = 1.3e-91
Identity = 171/186 (91.94%), Postives = 179/186 (96.24%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           MADE+VKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKR+MPSLYPHA GILYALK
Sbjct: 1   MADESVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKREMPSLYPHAKGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTF+DKLGI SMFVAQEIFSSWTHKTDHFQRIHSRT VPF  
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFVDKLGINSMFVAQEIFSSWTHKTDHFQRIHSRTGVPFNS 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILV NGVNLGALRQGL++Y+QNVNKIEKNKQKW K+T+N
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVGNGVNLGALRQGLTKYTQNVNKIEKNKQKWTKYTKN 180

Query: 181 STSSEK 187
           STSSEK
Sbjct: 181 STSSEK 186

[Close]

BLAST of Moc04g08820 vs. NCBI nr
Match: XP_023530435.1 (magnesium-dependent phosphatase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 346.3 bits (887), Expect = 1.7e-91
Identity = 171/186 (91.94%), Postives = 178/186 (95.70%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           MADE+VK EALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKR+MPSLYPHA GILYALK
Sbjct: 1   MADESVKTEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKREMPSLYPHAKGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTF+DKLGI SMFVAQEIFSSWTHKTDHFQRIHSRT VPF  
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFVDKLGINSMFVAQEIFSSWTHKTDHFQRIHSRTGVPFNS 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILV NGVNLGALRQGL++YSQNVNKIEKNKQKW K+T+N
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVGNGVNLGALRQGLTKYSQNVNKIEKNKQKWTKYTKN 180

Query: 181 STSSEK 187
           STSSEK
Sbjct: 181 STSSEK 186

[Close]

BLAST of Moc04g08820 vs. ExPASy Swiss-Prot
Match: Q86V88 (Magnesium-dependent phosphatase 1 OS=Homo sapiens OX=9606 GN=MDP1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-23
Identity = 63/158 (39.87%), Postives = 87/158 (55.06%), Query Frame = 0


Query: 20  LPKLVVFDLDYTLWPFYCE--------------CRSKRDMP-SLYPHATGILYALKDKGI 79
           LPKL VFDLDYTLWPF+ +               R +R     LYP    +L  L+  G+
Sbjct: 4   LPKLAVFDLDYTLWPFWVDTHVDPPFHKSSDGTVRDRRGQDVRLYPEVPEVLKRLQSLGV 63

Query: 80  DLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKCMLFF 139
             A ASR+   + A   ++   +   FV +EI+     K  HF+R+  +T +PF  M+FF
Sbjct: 64  PGAAASRTSEIEGANQLLELFDLFRYFVHREIYPG--SKITHFERLQQKTGIPFSQMIFF 123

Query: 140 DDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQ 163
           DDE RNI  VSK+GVT I + NG+NL  L QGL  +++
Sbjct: 124 DDERRNIVDVSKLGVTCIHIQNGMNLQTLSQGLETFAK 159

[Close]

BLAST of Moc04g08820 vs. ExPASy Swiss-Prot
Match: Q9D967 (Magnesium-dependent phosphatase 1 OS=Mus musculus OX=10090 GN=Mdp1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.5e-23
Identity = 60/158 (37.97%), Postives = 88/158 (55.70%), Query Frame = 0


Query: 20  LPKLVVFDLDYTLWPFYCE--------------CRSKRDMP-SLYPHATGILYALKDKGI 79
           LPKL VFDLDYTLWPF+ +               R +R     LYP    +L  L+  G+
Sbjct: 4   LPKLAVFDLDYTLWPFWVDTHVDPPFHKSSDGTVRDRRGQNIQLYPEVPEVLGRLQSLGV 63

Query: 80  DLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKCMLFF 139
            +A ASR+     A   ++   +   F+ +EI+     K  HF+R+H +T VPF  M+FF
Sbjct: 64  PVAAASRTSEIQGANQLLELFDLGKYFIQREIYPG--SKVTHFERLHHKTGVPFSQMVFF 123

Query: 140 DDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQ 163
           DDE+RNI  V ++GVT I + +G++L  L QGL  +++
Sbjct: 124 DDENRNIIDVGRLGVTCIHIRDGMSLQTLTQGLETFAK 159

[Close]

BLAST of Moc04g08820 vs. ExPASy Swiss-Prot
Match: O94279 (Putative magnesium-dependent phosphatase P8B7.31 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBP8B7.31 PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 2.9e-14
Identity = 55/156 (35.26%), Postives = 83/156 (53.21%), Query Frame = 0


Query: 21  PKLVVFDLDYTLWPFYCECR-------SKRDMPSL----------YPHATGILYALKDKG 80
           PK VVFDLDYTLWP + +         SK D   L          Y   TGIL  L+++ 
Sbjct: 8   PKCVVFDLDYTLWPLWIDTHVTAPFKPSKNDPGVLIDKYGTEICFYSDITGILQELRNQK 67

Query: 81  IDLAIASRSPTSDIAKTFIDKLGIK---SMFVAQEIFS---SWT-HKTDHFQRIHSRTNV 140
           + L +ASR+     AK  ++ + +    S+  A E F+   +W   K DHF+ IH+ + +
Sbjct: 68  VTLCVASRTCAPKYAKQALNLMKVPIDGSLKPAIEFFTYVKAWPGSKMDHFKEIHNESGI 127

Query: 141 PFKCMLFFDDEDRNIETVSKMGVTSI--LVDNGVNL 151
            ++ M+FFDDE RN E V ++GVT +  +  N +N+
Sbjct: 128 DYREMVFFDDESRNRE-VERLGVTFLEKIKKNSLNI 162

[Close]

BLAST of Moc04g08820 vs. ExPASy Swiss-Prot
Match: P40081 (Putative magnesium-dependent phosphatase YER134C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YER134C PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.1e-08
Identity = 45/161 (27.95%), Postives = 69/161 (42.86%), Query Frame = 0


Query: 21  PKLVVFDLDYTLWPFYCE---------------------CRSKRDMPSLYPHATGILYAL 80
           P +  FDLDYT+WP YC+                     CR   ++ ++Y     IL  L
Sbjct: 5   PDVAAFDLDYTIWPCYCDTHLHGPFKPVKSSNGEVLTIICRDGYEL-TIYKDIPRILGDL 64

Query: 81  KDKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIF-----------SSWTHKTDHFQ 140
           KD G+ L  ASR+   +IA+  +    +K   V   +            S   H  D  +
Sbjct: 65  KDNGVKLMTASRTWAPEIAQEILKIFKVKYAGVVTPLANLFDEFQWGERSKIGHLRDGLK 124

Query: 141 RIHSRTNVPFKCMLFFDDEDRNIETVSKMGVTSILVDNGVN 150
            +++ +++  K +  FDDE RN E V K GV  + V +  N
Sbjct: 125 DLYNTSDLKSKKICLFDDESRNKE-VEKYGVKFVYVRDPEN 163

[Close]

BLAST of Moc04g08820 vs. ExPASy TrEMBL
Match: A0A6J1C3D1 (magnesium-dependent phosphatase 1 OS=Momordica charantia OX=3673 GN=LOC111008105 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.2e-100
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK
Sbjct: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180

Query: 181 STSSEKK 188
           STSSEKK
Sbjct: 181 STSSEKK 187

[Close]

BLAST of Moc04g08820 vs. ExPASy TrEMBL
Match: A0A6J1JEG7 (magnesium-dependent phosphatase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111486164 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.6e-92
Identity = 172/186 (92.47%), Postives = 179/186 (96.24%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKR+MPSLYPHA GILYALK
Sbjct: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKREMPSLYPHAKGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTF+DKLGI SMFVAQEIFSSWTHKTDHFQRIHSRT VPF  
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFVDKLGINSMFVAQEIFSSWTHKTDHFQRIHSRTGVPFNS 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILV NGVNLGALRQGL++Y+QNVNKIEKNKQKW K+T+N
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVGNGVNLGALRQGLTKYTQNVNKIEKNKQKWTKYTKN 180

Query: 181 STSSEK 187
           STSSEK
Sbjct: 181 STSSEK 186

[Close]

BLAST of Moc04g08820 vs. ExPASy TrEMBL
Match: A0A6J1EQA4 (magnesium-dependent phosphatase 1 OS=Cucurbita moschata OX=3662 GN=LOC111434698 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.2e-92
Identity = 171/186 (91.94%), Postives = 179/186 (96.24%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           MADE+VKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKR+MPSLYPHA GILYALK
Sbjct: 1   MADESVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKREMPSLYPHAKGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTF+DKLGI SMFVAQEIFSSWTHKTDHFQRIHSRT VPF  
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFVDKLGINSMFVAQEIFSSWTHKTDHFQRIHSRTGVPFNS 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILV NGVNLGALRQGL++Y+QNVNKIEKNKQKW K+T+N
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVGNGVNLGALRQGLTKYTQNVNKIEKNKQKWTKYTKN 180

Query: 181 STSSEK 187
           STSSEK
Sbjct: 181 STSSEK 186

[Close]

BLAST of Moc04g08820 vs. ExPASy TrEMBL
Match: A0A5D3C6H7 (Magnesium-dependent phosphatase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002090 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.0e-91
Identity = 170/186 (91.40%), Postives = 178/186 (95.70%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           M D++VKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKR+MPS+YPHA GILYALK
Sbjct: 1   MGDDSVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKREMPSMYPHAKGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTFI KLGI+SMFVAQEIFSSWTHKTDHFQRIHSRT VPFK 
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFIGKLGIESMFVAQEIFSSWTHKTDHFQRIHSRTGVPFKS 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILV NGVNLGALRQGL+ YSQNVNKIEKNK+KW K+TQN
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVGNGVNLGALRQGLTSYSQNVNKIEKNKKKWTKYTQN 180

Query: 181 STSSEK 187
           STSSEK
Sbjct: 181 STSSEK 186

[Close]

BLAST of Moc04g08820 vs. ExPASy TrEMBL
Match: A0A1S3BZU1 (magnesium-dependent phosphatase 1 OS=Cucumis melo OX=3656 GN=LOC103495389 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.0e-91
Identity = 170/186 (91.40%), Postives = 178/186 (95.70%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           M D++VKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKR+MPS+YPHA GILYALK
Sbjct: 1   MGDDSVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKREMPSMYPHAKGILYALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           DKGIDLAIASRSPTSDIAKTFI KLGI+SMFVAQEIFSSWTHKTDHFQRIHSRT VPFK 
Sbjct: 61  DKGIDLAIASRSPTSDIAKTFIGKLGIESMFVAQEIFSSWTHKTDHFQRIHSRTGVPFKS 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWKKFTQN 180
           MLFFDDEDRNIETVSKMGVTSILV NGVNLGALRQGL+ YSQNVNKIEKNK+KW K+TQN
Sbjct: 121 MLFFDDEDRNIETVSKMGVTSILVGNGVNLGALRQGLTSYSQNVNKIEKNKKKWTKYTQN 180

Query: 181 STSSEK 187
           STSSEK
Sbjct: 181 STSSEK 186

[Close]

BLAST of Moc04g08820 vs. TAIR 10
Match: AT2G14110.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 8.6e-78
Identity = 139/186 (74.73%), Postives = 165/186 (88.71%), Query Frame = 0


Query: 1   MADENVKAEALQIIGMFQVLPKLVVFDLDYTLWPFYCECRSKRDMPSLYPHATGILYALK 60
           MA+E VK EA+QI+GMFQ+LP+LVVFDLDYTLWPFYCECRSKR+MPS+YP A GIL ALK
Sbjct: 1   MAEEKVKDEAMQIMGMFQILPRLVVFDLDYTLWPFYCECRSKREMPSMYPQAKGILSALK 60

Query: 61  DKGIDLAIASRSPTSDIAKTFIDKLGIKSMFVAQEIFSSWTHKTDHFQRIHSRTNVPFKC 120
           +KGI++AIASRSPTSDIA TF+DKL IK MFVA+EI+SSW+HKT+HFQ+IH+RT VPF  
Sbjct: 61  EKGIEMAIASRSPTSDIANTFLDKLNIKPMFVAKEIYSSWSHKTEHFQKIHTRTGVPFTA 120

Query: 121 MLFFDDEDRNIETVSKMGVTSILVDNGVNLGALRQGLSEYSQNVNKIEKNKQKWK-KFTQ 180
           MLFFDDEDRNI++VSKMGVTSILV +GV LGA RQGL+E++QN N IEKNKQ W+ K++ 
Sbjct: 121 MLFFDDEDRNIKSVSKMGVTSILVGDGVTLGAFRQGLTEFTQNHNSIEKNKQVWRDKYSG 180

Query: 181 NSTSSE 186
             TSSE
Sbjct: 181 KPTSSE 186

The following BLAST results are available for this feature:

BLAST of Moc04g08820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136381.1	2.6e-100	100.00	magnesium-dependent phosphatase 1 [Momordica charantia]	[more]
XP_022988962.1	3.4e-92	92.47	magnesium-dependent phosphatase 1-like [Cucurbita maxima]	[more]
XP_022927945.1	1.3e-91	91.94	magnesium-dependent phosphatase 1 [Cucurbita moschata] >KAG6588747.1 Magnesium-d...	[more]
KAG7022531.1	1.3e-91	91.94	Beta-ureidopropionase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023530435.1	1.7e-91	91.94	magnesium-dependent phosphatase 1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc04g08820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q86V88	1.2e-23	39.87	Magnesium-dependent phosphatase 1 OS=Homo sapiens OX=9606 GN=MDP1 PE=1 SV=1	[more]
Q9D967	4.5e-23	37.97	Magnesium-dependent phosphatase 1 OS=Mus musculus OX=10090 GN=Mdp1 PE=1 SV=1	[more]
O94279	2.9e-14	35.26	Putative magnesium-dependent phosphatase P8B7.31 OS=Schizosaccharomyces pombe (s...	[more]
P40081	9.1e-08	27.95	Putative magnesium-dependent phosphatase YER134C OS=Saccharomyces cerevisiae (st...	[more]

BLAST of Moc04g08820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C3D1	1.2e-100	100.00	magnesium-dependent phosphatase 1 OS=Momordica charantia OX=3673 GN=LOC111008105...	[more]
A0A6J1JEG7	1.6e-92	92.47	magnesium-dependent phosphatase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114861...	[more]
A0A6J1EQA4	6.2e-92	91.94	magnesium-dependent phosphatase 1 OS=Cucurbita moschata OX=3662 GN=LOC111434698 ...	[more]
A0A5D3C6H7	4.0e-91	91.40	Magnesium-dependent phosphatase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3BZU1	4.0e-91	91.40	magnesium-dependent phosphatase 1 OS=Cucumis melo OX=3656 GN=LOC103495389 PE=4 S...	[more]

BLAST of Moc04g08820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G14110.1	8.6e-78	74.73	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 150..170
None	No IPR available	PIRSR	PIRSR006118-2	PIRSR006118-2	coord: 57..147 e-value: 0.0063 score: 14.0
None	No IPR available	SFLD	SFLDG01129	C1.5:_HAD,_Beta-PGM,_Phosphat	coord: 3..167 e-value: 0.0 score: 184.0
None	No IPR available	SFLD	SFLDG01131	C1.5.2:_MDP_Like	coord: 3..167 e-value: 0.0 score: 184.0
None	No IPR available	PANTHER	PTHR17901:SF22	MAGNESIUM-DEPENDENT PHOSPHATASE 1-LIKE	coord: 1..177
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 3..166 e-value: 2.4E-50 score: 172.6
IPR010033	HAD-superfamily phosphatase, subfamily IIIC	TIGRFAM	TIGR01681	TIGR01681	coord: 22..137 e-value: 8.6E-14 score: 50.5
IPR010036	Magnesium-dependent phosphatase-1, eukaryotic/archaeal-type	PFAM	PF12689	Acid_PPase	coord: 47..162 e-value: 2.4E-27 score: 95.8 coord: 20..41 e-value: 8.0E-7 score: 29.1
IPR010036	Magnesium-dependent phosphatase-1, eukaryotic/archaeal-type	PANTHER	PTHR17901	MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1	coord: 1..177
IPR035679	Magnesium-dependent phosphatase-1, eukaryotic-type	CDD	cd07501	HAD_MDP-1_like	coord: 21..144 e-value: 2.63159E-56 score: 172.146
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 21..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g08820.1	Moc04g08820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035335	peptidyl-tyrosine dephosphorylation
molecular_function	GO:0003993	acid phosphatase activity
molecular_function	GO:0004725	protein tyrosine phosphatase activity
molecular_function	GO:0016791	phosphatase activity