Moc04g08250 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g08250
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	E3 ubiquitin-protein ligase RING1-like
Location	chr4: 5967228 .. 5967956 (+)
RNA-Seq Expression	Moc04g08250
Synteny	Moc04g08250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAATCAAAGTCAAAAATGGCATCGATCCTGATCTTGAATTAATATCAATATCAATGGTGGAAAAAATCAGGCTGAAACTCCATGTACAGGCCCAATACGATCATTTCCAAGTCTTAGGGGAACATGCTCCAACATTGGTGAGCCATCGCCAAACCCCTACGTTTTGCTTCGCTGATTACCAACTTCCAGAGTGCATCTTCAACCGTGGCATTCGCAACAAGACTCTACAACGCTTCCTATCATTGCAATTCAAAGAATATGAAGACTCCATTAACATACAAGCCATGACACACCAAATAATAAATCATTGGATCCATGTCAAACGAGAAGCAGCTGCAGCAGCAACTCATGATCATAATGATAATTCCAATTCCAGTGCATTTGGGAGAGTTTTTCCCCTTGAAGTTACCCTGCATTTGTGGAACTCAGTCATTCACTATGATGATGATGATTATTATGCTATGATTCCAGCAAGCGAAGTTGGTATTGAGAAGATGTTAAAGACCGTAACGTTTGAGGAGATTGCACCGGAATCAACAACTACTTGTGTCATTTGCTTGGAGGAGATCACCAACCAAGATATGGATAGTGGAATGTCATTACTGCAGATGCCTTGCTTGCATGTGTTTCATGGAAGATGCATTAAGAAGTGGCTTAACAACAGTCATTATTGTCCCACTTGTAGATTTCAAATGCCTACCAATTCAACAAATTCTCATCAATAG

mRNA sequence

ATGTCAATCAAAGTCAAAAATGGCATCGATCCTGATCTTGAATTAATATCAATATCAATGGTGGAAAAAATCAGGCTGAAACTCCATGTACAGGCCCAATACGATCATTTCCAAGTCTTAGGGGAACATGCTCCAACATTGGTGAGCCATCGCCAAACCCCTACGTTTTGCTTCGCTGATTACCAACTTCCAGAGTGCATCTTCAACCGTGGCATTCGCAACAAGACTCTACAACGCTTCCTATCATTGCAATTCAAAGAATATGAAGACTCCATTAACATACAAGCCATGACACACCAAATAATAAATCATTGGATCCATGTCAAACGAGAAGCAGCTGCAGCAGCAACTCATGATCATAATGATAATTCCAATTCCAGTGCATTTGGGAGAGTTTTTCCCCTTGAAGTTACCCTGCATTTGTGGAACTCAGTCATTCACTATGATGATGATGATTATTATGCTATGATTCCAGCAAGCGAAGTTGGTATTGAGAAGATGTTAAAGACCGTAACGTTTGAGGAGATTGCACCGGAATCAACAACTACTTGTGTCATTTGCTTGGAGGAGATCACCAACCAAGATATGGATAGTGGAATGTCATTACTGCAGATGCCTTGCTTGCATGTGTTTCATGGAAGATGCATTAAGAAGTGGCTTAACAACAGTCATTATTGTCCCACTTGTAGATTTCAAATGCCTACCAATTCAACAAATTCTCATCAATAG

Coding sequence (CDS)

ATGTCAATCAAAGTCAAAAATGGCATCGATCCTGATCTTGAATTAATATCAATATCAATGGTGGAAAAAATCAGGCTGAAACTCCATGTACAGGCCCAATACGATCATTTCCAAGTCTTAGGGGAACATGCTCCAACATTGGTGAGCCATCGCCAAACCCCTACGTTTTGCTTCGCTGATTACCAACTTCCAGAGTGCATCTTCAACCGTGGCATTCGCAACAAGACTCTACAACGCTTCCTATCATTGCAATTCAAAGAATATGAAGACTCCATTAACATACAAGCCATGACACACCAAATAATAAATCATTGGATCCATGTCAAACGAGAAGCAGCTGCAGCAGCAACTCATGATCATAATGATAATTCCAATTCCAGTGCATTTGGGAGAGTTTTTCCCCTTGAAGTTACCCTGCATTTGTGGAACTCAGTCATTCACTATGATGATGATGATTATTATGCTATGATTCCAGCAAGCGAAGTTGGTATTGAGAAGATGTTAAAGACCGTAACGTTTGAGGAGATTGCACCGGAATCAACAACTACTTGTGTCATTTGCTTGGAGGAGATCACCAACCAAGATATGGATAGTGGAATGTCATTACTGCAGATGCCTTGCTTGCATGTGTTTCATGGAAGATGCATTAAGAAGTGGCTTAACAACAGTCATTATTGTCCCACTTGTAGATTTCAAATGCCTACCAATTCAACAAATTCTCATCAATAG

Protein sequence

MSIKVKNGIDPDLELISISMVEKIRLKLHVQAQYDHFQVLGEHAPTLVSHRQTPTFCFADYQLPECIFNRGIRNKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAAAATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQMPTNSTNSHQ

Homology

[Close]

BLAST of Moc04g08250 vs. NCBI nr
Match: XP_022158635.1 (RING-H2 finger protein ATL47-like [Momordica charantia])

HSP 1 Score: 340.9 bits (873), Expect = 9.1e-90
Identity = 164/190 (86.32%), Postives = 170/190 (89.47%), Query Frame = 0


Query: 56  FCFADYQLPECIFNRGIRNKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAAA 115
           FCF DYQLPECI NRGI +KTLQRFLS QFKEYEDSINIQ MTHQIINHW+HVKRE  AA
Sbjct: 2   FCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVAA 61

Query: 116 ATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEE 175
            T DHNDNSNS+AFGRVFPLEVTLHLWNSVIHYD D  YAM+PASEVGIEKMLKTV FEE
Sbjct: 62  TTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYD--YAMVPASEVGIEKMLKTVKFEE 121

Query: 176 IAPESTTTCVICLEEIT---NQDMDSGMSLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQ 235
           I  ES TTCVICLEEIT   NQD+DSGM LLQ+PCLHVFHGRCIKKWLNNSHYCPTCRFQ
Sbjct: 122 IVLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHYCPTCRFQ 181

Query: 236 MPTNSTNSHQ 243
           MPTNSTNSHQ
Sbjct: 182 MPTNSTNSHQ 189

[Close]

BLAST of Moc04g08250 vs. NCBI nr
Match: XP_022158633.1 (E3 ubiquitin-protein ligase RING1-like [Momordica charantia])

HSP 1 Score: 243.4 bits (620), Expect = 2.0e-60
Identity = 122/148 (82.43%), Postives = 129/148 (87.16%), Query Frame = 0


Query: 97  MTHQIINHWIHVKREAAAAA-THDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYA 156
           MT QIINHW+HVKREAAAAA T D N+NSNS AFGRVFPLEVTLHLWNSVI  DDDD YA
Sbjct: 1   MTXQIINHWLHVKREAAAAATTRDRNNNSNSIAFGRVFPLEVTLHLWNSVIE-DDDDDYA 60

Query: 157 MIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEIT---NQDMDSGMSLLQMPCLHVFH 216
           M+PASEVGIEKMLKTV FEEI  ES TTCVICLEEIT   NQD+DSGM L+QMPCLHVFH
Sbjct: 61  MVPASEVGIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLVQMPCLHVFH 120

Query: 217 GRCIKKWLNNSHYCPTCRFQMPTNSTNS 241
            +CI KWLNNSHYCPTCRFQMPTN TN+
Sbjct: 121 KKCILKWLNNSHYCPTCRFQMPTNLTNA 147

[Close]

BLAST of Moc04g08250 vs. NCBI nr
Match: XP_022158634.1 (E3 ubiquitin-protein ligase RING1-like [Momordica charantia])

HSP 1 Score: 228.8 bits (582), Expect = 5.0e-56
Identity = 114/144 (79.17%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 97  MTHQIINHWIHVKREAAAA-ATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYA 156
           MTHQIINHW+HVKREAAAA AT DHN+NSNS AFGRVF LEVT HLWNSVI  DDDD YA
Sbjct: 1   MTHQIINHWLHVKREAAAASATRDHNNNSNSIAFGRVFSLEVTFHLWNSVIE-DDDDDYA 60

Query: 157 MIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEIT---NQDMDSGMSLLQMPCLHVFH 216
           M+PASEV IEKMLKTV FEEI  ES TTCVICLEEIT   NQD+D+GM L+ +PCLHVFH
Sbjct: 61  MVPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDNGMLLVHIPCLHVFH 120

Query: 217 GRCIKKWLNNSHYCPTCRFQMPTN 237
             CI KWLNNSHYCPTCR +MPTN
Sbjct: 121 HICILKWLNNSHYCPTCRCEMPTN 143

[Close]

BLAST of Moc04g08250 vs. NCBI nr
Match: ADN34252.1 (hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putative zinc finger protein [Cucumis melo var. makuwa] >TYK06863.1 putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 166.0 bits (419), Expect = 4.0e-37
Identity = 93/233 (39.91%), Postives = 133/233 (57.08%), Query Frame = 0


Query: 17  SISMVEKIRLKLHVQAQYDHFQVL---GEHAPTLVSHRQTPTFCFADYQLPECIFNRGIR 76
           S ++VE+I LK  VQ +  HFQV      H+ TLV         FA +QLP  IF  G  
Sbjct: 41  SPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHG-- 100

Query: 77  NKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAAAATHDHNDNSNSSAFGRVF 136
           +KTL+R L  +FK Y D INI+ + ++II HW+    E       +  +N+++  F +++
Sbjct: 101 DKTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEE------EEDQENNSNVLFKKIY 160

Query: 137 PLEVTLHLWNSVI------HYDDDDYYAMIPASEVGIEKMLKTVTFEEI---APESTTTC 196
           PLE+ + L  S +           D   M+P S+  +E MLK V  EEI     + +  C
Sbjct: 161 PLEIMIRLLVSKMIRAVQPRLAMIDQPLMVPTSDSAVESMLKRVENEEIMKSGDDESINC 220

Query: 197 VICLEEITNQDMDSGMSLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQMPTNS 238
           V+CLEEI+ ++  S  ++LQMPCLH+FH  CI+KWL  SHYCPTCRF MP ++
Sbjct: 221 VVCLEEISKEEKGSETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of Moc04g08250 vs. NCBI nr
Match: KGN43799.1 (hypothetical protein Csa_017222 [Cucumis sativus])

HSP 1 Score: 154.1 bits (388), Expect = 1.6e-33
Identity = 104/266 (39.10%), Postives = 139/266 (52.26%), Query Frame = 0


Query: 1   MSIKVKNGIDPDLEL----------ISISMVEKIRLKLHVQAQYDHFQVLGE---HAPTL 60
           MS+++    DPDL L           S + V++I LKL V+ +  HFQ   E    A TL
Sbjct: 18  MSLRI---TDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQHFQADEEGDTFASTL 77

Query: 61  -----VSHRQTPTFCFADYQLPECIFNRGIRNKTLQRFLSLQFKEYEDSINIQAMTHQII 120
                VSH   PTF     QLP  +F  G  N+TL+R L  QF+ +   INIQ +  +II
Sbjct: 78  MIEHIVSHHPFPTF-----QLPVSVFRHG--NRTLKRLLFQQFQIFRGIINIQLVADEII 137

Query: 121 NHWIHVKREAAAAATHDHNDNSNSSAFGRVFPLEVT--------LHLWNSVIHYDDDDYY 180
            HW  VK+E       +  +N++      ++PLE+T        +H  +      D    
Sbjct: 138 EHW--VKKE-------EDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQV 197

Query: 181 AMIPASEVGIEKMLKTVTFEEI---APESTTTCVICLEEITNQDMDSGMSLLQMPCLHVF 238
            M+PASE  +E MLK V  EEI     + +  CVICLEEI+ +   S   +LQMPCLH+F
Sbjct: 198 TMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKMKGSEGVVLQMPCLHMF 257

[Close]

BLAST of Moc04g08250 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 0


Query: 149 DDDDYYAMIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLLQMPCL 208
           +D + Y   PAS+  I+ +      +++       C +C++E      + G  + QMPC 
Sbjct: 181 NDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDE-----FEDGSDVKQMPCK 240

Query: 209 HVFHGRCIKKWLNNSHYCPTCRFQMPTN 237
           HVFH  C+  WL   + CP CRF++PT+
Sbjct: 241 HVFHQDCLLPWLELHNSCPVCRFELPTD 263

[Close]

BLAST of Moc04g08250 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.8e-09
Identity = 34/100 (34.00%), Postives = 49/100 (49.00%), Query Frame = 0


Query: 142 WNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEEIAPES-TTTCVICLEEITNQDMDSGM 201
           +N +   D  + Y  +PA+   +  +     F   A  +    CVIC EE     M  G 
Sbjct: 172 YNHLRRRDQKEGY-QVPAARAAVVALRAVEVFNVAASNAGEVECVICKEE-----MSEGR 231

Query: 202 SLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQMPTNSTNS 241
            + +MPC H FH +CI  WL+  + CP CRFQ+PT+   S
Sbjct: 232 DVCEMPCQHFFHWKCILPWLSKKNTCPFCRFQLPTDDVFS 265

[Close]

BLAST of Moc04g08250 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.8e-08
Identity = 32/107 (29.91%), Postives = 57/107 (53.27%), Query Frame = 0


Query: 135 LEVTLHLWNSVIHYD-----DDDYYAMIPASEVGIEKMLKTVTFEEIAPESTTTCVICLE 194
           LE+   L+N +   D     D D++   PA++  +E + +TV       ++   C +CL 
Sbjct: 25  LELARSLFNRMDFEDLGLVVDWDHHLPPPAAKTAVENLPRTVI---RGSQAELKCPVCLL 84

Query: 195 EITNQDMDSGMSLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQMPTN 237
           E   ++     + ++MPC H+FH  CI  WL+ ++ CP CR ++PT+
Sbjct: 85  EFEEEE-----TAIEMPCHHLFHSNCILPWLSKTNSCPLCRHELPTD 123

[Close]

BLAST of Moc04g08250 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.8e-08
Identity = 32/107 (29.91%), Postives = 58/107 (54.21%), Query Frame = 0


Query: 135 LEVTLHLWNSVIHYD-----DDDYYAMIPASEVGIEKMLKTVTFEEIAPESTTTCVICLE 194
           LE+   L+N +   D     D D++   PA++  +E + +TV       ++   C +CL 
Sbjct: 25  LELARSLFNRMDFEDLGLVVDWDHHLPPPAAKTVVENLPRTVI---RGSQAELKCPVCLL 84

Query: 195 EITNQDMDSGMSLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQMPTN 237
           E   ++     + ++MPC H+FH  CI  WL+ ++ CP CR+++PT+
Sbjct: 85  EFEEEE-----TAIEMPCHHLFHSSCILPWLSKTNSCPLCRYELPTD 123

[Close]

BLAST of Moc04g08250 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.4e-08
Identity = 37/120 (30.83%), Postives = 52/120 (43.33%), Query Frame = 0


Query: 119 DHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEEIAP 178
           D   ++N       + L   L L    +   D       PA +  +E  L TV  EE+  
Sbjct: 140 DQGGSTNDDGLLEEYVLGAGLSLLLQHLAESDPSRNGTPPAKKEAVE-ALPTVKIEEV-- 199

Query: 179 ESTTTCVICLEEITNQDMDSGMSLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQMPTNST 238
               +C +CL+     D++ G    QMPC H FH  CI  WL     CP CRF++P+  T
Sbjct: 200 ---VSCSVCLD-----DLEVGSQAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPSEET 248

[Close]

BLAST of Moc04g08250 vs. ExPASy TrEMBL
Match: A0A6J1DWD8 (RING-H2 finger protein ATL47-like OS=Momordica charantia OX=3673 GN=LOC111025085 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 4.4e-90
Identity = 164/190 (86.32%), Postives = 170/190 (89.47%), Query Frame = 0


Query: 56  FCFADYQLPECIFNRGIRNKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAAA 115
           FCF DYQLPECI NRGI +KTLQRFLS QFKEYEDSINIQ MTHQIINHW+HVKRE  AA
Sbjct: 2   FCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVAA 61

Query: 116 ATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEE 175
            T DHNDNSNS+AFGRVFPLEVTLHLWNSVIHYD D  YAM+PASEVGIEKMLKTV FEE
Sbjct: 62  TTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYD--YAMVPASEVGIEKMLKTVKFEE 121

Query: 176 IAPESTTTCVICLEEIT---NQDMDSGMSLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQ 235
           I  ES TTCVICLEEIT   NQD+DSGM LLQ+PCLHVFHGRCIKKWLNNSHYCPTCRFQ
Sbjct: 122 IVLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHYCPTCRFQ 181

Query: 236 MPTNSTNSHQ 243
           MPTNSTNSHQ
Sbjct: 182 MPTNSTNSHQ 189

[Close]

BLAST of Moc04g08250 vs. ExPASy TrEMBL
Match: A0A6J1DWN3 (E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC111025082 PE=4 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 9.6e-61
Identity = 122/148 (82.43%), Postives = 129/148 (87.16%), Query Frame = 0


Query: 97  MTHQIINHWIHVKREAAAAA-THDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYA 156
           MT QIINHW+HVKREAAAAA T D N+NSNS AFGRVFPLEVTLHLWNSVI  DDDD YA
Sbjct: 1   MTXQIINHWLHVKREAAAAATTRDRNNNSNSIAFGRVFPLEVTLHLWNSVIE-DDDDDYA 60

Query: 157 MIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEIT---NQDMDSGMSLLQMPCLHVFH 216
           M+PASEVGIEKMLKTV FEEI  ES TTCVICLEEIT   NQD+DSGM L+QMPCLHVFH
Sbjct: 61  MVPASEVGIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLVQMPCLHVFH 120

Query: 217 GRCIKKWLNNSHYCPTCRFQMPTNSTNS 241
            +CI KWLNNSHYCPTCRFQMPTN TN+
Sbjct: 121 KKCILKWLNNSHYCPTCRFQMPTNLTNA 147

[Close]

BLAST of Moc04g08250 vs. ExPASy TrEMBL
Match: A0A6J1DZZ7 (E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC111025083 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 2.4e-56
Identity = 114/144 (79.17%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 97  MTHQIINHWIHVKREAAAA-ATHDHNDNSNSSAFGRVFPLEVTLHLWNSVIHYDDDDYYA 156
           MTHQIINHW+HVKREAAAA AT DHN+NSNS AFGRVF LEVT HLWNSVI  DDDD YA
Sbjct: 1   MTHQIINHWLHVKREAAAASATRDHNNNSNSIAFGRVFSLEVTFHLWNSVIE-DDDDDYA 60

Query: 157 MIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEIT---NQDMDSGMSLLQMPCLHVFH 216
           M+PASEV IEKMLKTV FEEI  ES TTCVICLEEIT   NQD+D+GM L+ +PCLHVFH
Sbjct: 61  MVPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDNGMLLVHIPCLHVFH 120

Query: 217 GRCIKKWLNNSHYCPTCRFQMPTN 237
             CI KWLNNSHYCPTCR +MPTN
Sbjct: 121 HICILKWLNNSHYCPTCRCEMPTN 143

[Close]

BLAST of Moc04g08250 vs. ExPASy TrEMBL
Match: A0A5D3C4K6 (Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001670 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.9e-37
Identity = 93/233 (39.91%), Postives = 133/233 (57.08%), Query Frame = 0


Query: 17  SISMVEKIRLKLHVQAQYDHFQVL---GEHAPTLVSHRQTPTFCFADYQLPECIFNRGIR 76
           S ++VE+I LK  VQ +  HFQV      H+ TLV         FA +QLP  IF  G  
Sbjct: 41  SPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHG-- 100

Query: 77  NKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAAAATHDHNDNSNSSAFGRVF 136
           +KTL+R L  +FK Y D INI+ + ++II HW+    E       +  +N+++  F +++
Sbjct: 101 DKTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEE------EEDQENNSNVLFKKIY 160

Query: 137 PLEVTLHLWNSVI------HYDDDDYYAMIPASEVGIEKMLKTVTFEEI---APESTTTC 196
           PLE+ + L  S +           D   M+P S+  +E MLK V  EEI     + +  C
Sbjct: 161 PLEIMIRLLVSKMIRAVQPRLAMIDQPLMVPTSDSAVESMLKRVENEEIMKSGDDESINC 220

Query: 197 VICLEEITNQDMDSGMSLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQMPTNS 238
           V+CLEEI+ ++  S  ++LQMPCLH+FH  CI+KWL  SHYCPTCRF MP ++
Sbjct: 221 VVCLEEISKEEKGSETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of Moc04g08250 vs. ExPASy TrEMBL
Match: E5GCQ3 (Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.9e-37
Identity = 93/233 (39.91%), Postives = 133/233 (57.08%), Query Frame = 0


Query: 17  SISMVEKIRLKLHVQAQYDHFQVL---GEHAPTLVSHRQTPTFCFADYQLPECIFNRGIR 76
           S ++VE+I LK  VQ +  HFQV      H+ TLV         FA +QLP  IF  G  
Sbjct: 41  SPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHG-- 100

Query: 77  NKTLQRFLSLQFKEYEDSINIQAMTHQIINHWIHVKREAAAAATHDHNDNSNSSAFGRVF 136
           +KTL+R L  +FK Y D INI+ + ++II HW+    E       +  +N+++  F +++
Sbjct: 101 DKTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEE------EEDQENNSNVLFKKIY 160

Query: 137 PLEVTLHLWNSVI------HYDDDDYYAMIPASEVGIEKMLKTVTFEEI---APESTTTC 196
           PLE+ + L  S +           D   M+P S+  +E MLK V  EEI     + +  C
Sbjct: 161 PLEIMIRLLVSKMIRAVQPRLAMIDQPLMVPTSDSAVESMLKRVENEEIMKSGDDESINC 220

Query: 197 VICLEEITNQDMDSGMSLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQMPTNS 238
           V+CLEEI+ ++  S  ++LQMPCLH+FH  CI+KWL  SHYCPTCRF MP ++
Sbjct: 221 VVCLEEISKEEKGSETTVLQMPCLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of Moc04g08250 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.5e-10
Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 0


Query: 149 DDDDYYAMIPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLLQMPCL 208
           +D + Y   PAS+  I+ +      +++       C +C++E      + G  + QMPC 
Sbjct: 181 NDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDE-----FEDGSDVKQMPCK 240

Query: 209 HVFHGRCIKKWLNNSHYCPTCRFQMPTN 237
           HVFH  C+  WL   + CP CRF++PT+
Sbjct: 241 HVFHQDCLLPWLELHNSCPVCRFELPTD 263

[Close]

BLAST of Moc04g08250 vs. TAIR 10
Match: AT5G02750.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 3.4e-10
Identity = 34/100 (34.00%), Postives = 49/100 (49.00%), Query Frame = 0


Query: 142 WNSVIHYDDDDYYAMIPASEVGIEKMLKTVTFEEIAPES-TTTCVICLEEITNQDMDSGM 201
           +N +   D  + Y  +PA+   +  +     F   A  +    CVIC EE     M  G 
Sbjct: 172 YNHLRRRDQKEGY-QVPAARAAVVALRAVEVFNVAASNAGEVECVICKEE-----MSEGR 231

Query: 202 SLLQMPCLHVFHGRCIKKWLNNSHYCPTCRFQMPTNSTNS 241
            + +MPC H FH +CI  WL+  + CP CRFQ+PT+   S
Sbjct: 232 DVCEMPCQHFFHWKCILPWLSKKNTCPFCRFQLPTDDVFS 265

[Close]

BLAST of Moc04g08250 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-09
Identity = 28/79 (35.44%), Postives = 44/79 (55.70%), Query Frame = 0


Query: 158 PASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLLQMPCLHVFHGRCIK 217
           PAS+  IE  L  V   +   ++   C +C E       ++G+   +MPC H+FHG CI 
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAEANCAVCTEV-----FEAGIEGREMPCKHIFHGDCIV 249

Query: 218 KWLNNSHYCPTCRFQMPTN 237
            WL+  + CP CRF++P++
Sbjct: 250 PWLSIRNSCPVCRFELPSD 262

[Close]

BLAST of Moc04g08250 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 4.9e-09
Identity = 32/79 (40.51%), Postives = 45/79 (56.96%), Query Frame = 0


Query: 158 PASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLLQMPCLHVFHGRCIK 217
           PAS+  I+ M   V  +    E    CVICLEE  +++     ++ +MPC H FHG CI+
Sbjct: 92  PASKASIDAM-PIVEIDGCEGE----CVICLEEWKSEE-----TVKEMPCKHRFHGGCIE 151

Query: 218 KWLNNSHYCPTCRFQMPTN 237
           KWL     CP CR++MP +
Sbjct: 152 KWLGFHGSCPVCRYEMPVD 160

[Close]

BLAST of Moc04g08250 vs. TAIR 10
Match: AT3G60080.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.1e-08
Identity = 26/81 (32.10%), Postives = 43/81 (53.09%), Query Frame = 0


Query: 157 IPASEVGIEKMLKTVTFEEIAPESTTTCVICLEEITNQDMDSGMSLLQMPCLHVFHGRCI 216
           IP  ++    +  T   ++  P+S   C +C E     D   G S  ++PC H++H  CI
Sbjct: 145 IPTIQISSSLLCST---DDSDPDSVLLCAVCKE-----DFIIGESARRLPCSHIYHSDCI 204

Query: 217 KKWLNNSHYCPTCRFQMPTNS 238
             WL++ + CP CRF++PT +
Sbjct: 205 VPWLSDHNSCPLCRFELPTTA 217

The following BLAST results are available for this feature:

BLAST of Moc04g08250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158635.1	9.1e-90	86.32	RING-H2 finger protein ATL47-like [Momordica charantia]	[more]
XP_022158633.1	2.0e-60	82.43	E3 ubiquitin-protein ligase RING1-like [Momordica charantia]	[more]
XP_022158634.1	5.0e-56	79.17	E3 ubiquitin-protein ligase RING1-like [Momordica charantia]	[more]
ADN34252.1	4.0e-37	39.91	hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putati...	[more]
KGN43799.1	1.6e-33	39.10	hypothetical protein Csa_017222 [Cucumis sativus]	[more]

BLAST of Moc04g08250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	2.2e-09	31.82	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q8GXF8	4.8e-09	34.00	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
Q3T0W3	1.8e-08	29.91	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]
Q9P0P0	1.8e-08	29.91	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]
Q6AVN2	2.4e-08	30.83	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]

BLAST of Moc04g08250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWD8	4.4e-90	86.32	RING-H2 finger protein ATL47-like OS=Momordica charantia OX=3673 GN=LOC111025085...	[more]
A0A6J1DWN3	9.6e-61	82.43	E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1DZZ7	2.4e-56	79.17	E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5D3C4K6	1.9e-37	39.91	Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
E5GCQ3	1.9e-37	39.91	Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]

BLAST of Moc04g08250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	1.5e-10	31.82	RING/U-box superfamily protein	[more]
AT5G02750.1	3.4e-10	34.00	RING/U-box superfamily protein	[more]
AT3G46620.1	2.9e-09	35.44	zinc finger (C3HC4-type RING finger) family protein	[more]
AT1G26800.1	4.9e-09	40.51	RING/U-box superfamily protein	[more]
AT3G60080.1	1.1e-08	32.10	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 184..229 e-value: 1.6E-7 score: 41.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 183..230 e-value: 7.8E-12 score: 45.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 184..230 score: 12.887609
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 183..230 e-value: 0.0097 score: 7.3
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 147..236 e-value: 1.8E-18 score: 67.9
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 116..235
None	No IPR available	PANTHER	PTHR15710:SF63	RING/U-BOX SUPERFAMILY PROTEIN	coord: 116..235
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 166..235

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g08250.1	Moc04g08250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding