Moc04g08220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g08220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RING-H2 finger protein ATL47-like
Location	chr4: 5947609 .. 5951701 (-)
RNA-Seq Expression	Moc04g08220
Synteny	Moc04g08220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTGCTTCGTCGATTACCAACTTCCAGAGTGCATCATCAACCGTGGCATTGGCGACAAGACTCTGCAGCGCTTCCTATCATGGCAGTTCAAAGAATATGAAGACTCCATTAACATACAAGTTATGACACACCAAATAATAAACCACTGGCTCCATGTCAAACGAGAAGGAGTTGCAGCAACAACTCAGGATCATAATGATAATTCCAATTCCAATGCGTTTGGGAGAGTTTTTCCCCTTGAAGTTACCCTGCATTTGTGGAATTCAGTCATTCATTATGATTATGATTATGCTATGGTTCCAGCAAGCGAAGTTGGTATTGAGAAGATGTTAAAGACCGTAAAGTTTGAGGAGATTGTACTGGAATCAGCAACTACTTGTGTCATTTGCTTGGAGGAGATCACCACGATGCCCAACCAAGATATCGACAGTGGAATGTTATTACTGCAAATACCTTGCTTGCATGTGTTTCATGGAAGATGCATCAAGAAGTGGCTTAACAACAGTCATTATTGTCCCACTTGTAGATTTCAAATGCCTACCAATTCAACAAATTCTCATCAATAGATTTATAAAAAATATATAATGTGCTGTTTTTTAAATACGACATGTATTATGTTATGGATTTGTTTCCTACTATACACATCTTGATCTCATTCTTGTCAATTTCAAAATTAGTTGTTGTAAATATTAAATACTGTTATTGTGACCAAATTATAACCTTAGTTTTTAAAAGAGTAACAGAGAATCAATCAGACACTTTCTACTTTTTAAAATCCACTAATAATATATATAAGCACCTGATAAAATCTTATATAGTTTTTATAAACATTTTCAAAATGTATGCCAAAATTTGAAAATCAACAAAGTAGTTTTCAAAATTTTGTTTTTATTTTTGAAATTGGACTACAAATTCAAGTCTTTTAAGAGGTGAAAACTAAATTGTACGGAAAAAAACAAGAACATTAAGGAAATGGGAATAAGCTCATGCCTCCTATATATAAATGTCTTAATGTCTCATAATTTTCATCATTCAAAATTCTGATCTCATCTTCACTCTGAATCAAAGTATTTAGTTGGTTGTTGAATCTTGTGTGAATAAAATATAATTCATGGAGCATCTCCGCGATCTTCTTGGAGATTCTACAGTGTGCATGTCAATCAAAGTCAAAAGTGGCACCGATCCTGATCCTGAATTAATATTAATATCAATGGTGGAAAAAATCAAGTTGAAACTCATGTACAGGCCCAATACGATCATTTCCAAGTCTTAGGGGAACATGCTCCAACATTGGTGAGCCATCGACAAACCCCTACGTTTTGCTTCACTGATTACCAACGTCCAGAGTGCATCTTCAACCGTGGCACTGGCAACAAAACTCTACAGCGCTTCCTATCATGGCAGTTCAAAGAATATGAAGACTTCGTTAACATACAAGCCATGACACACCAAAATTGGAAGAGTAGGGGGAGAGAAAGAGAGAAATACGGAAGTGAGAGAAAGATATTAAGAGAAATTAAAGAGAAATATTTTATATTTTAAAAATATATATATTTTCTATTTTCTACTTTCATCTCTAAATTCATCATTTCAATTTCAATTTAATATTATTTTAATTTTTGTTATTTTTATATATTTTTTAATTTTTAATAACAAATAACAAATGTTGCACGACAATATCAAATAAAATTAAAATGATCCAGACGAAATACACTATCTACAAGTAGCATAGGACGAGATTGAAGATGTCCTTTCCCATTCTCGTACTCGTAAAAAATTTTAATCTTTCGTCTTCACTTCGTTCTTCGATCCGAGAATCAAGATCCATGTTAAAGAACACTTATTTGGCGATGGGATATTCGAGAGAAAACTTACCGGTTTTATAATTAATTTTAATTATTTTTAGAATTTAAAAATAAAAAAGTTTAATAAATATATATTAGTTATTTTAGAATTATAATTTACTAGTATAAATAATTAGTTTTAAAAAATATTTAATATATTATTCATAATTTAGTAAATATGTCAATAAAATATATATTAATTATTTAAAAAATACAATCGGTGACCGAGACTGGGTCAGAGACCTGTATTTCCTATTTTTGAAGCAGAGACTCAATCCTGCGGGAAATTTTATCATCCCTAGAGTAGAGATATGAAACTACAAGTTTAGAGAGATGAAAATAAACTTTTGAAAGAGAAAATTTAGAGAGACAGGGAAAAAAATTTAGAAAAAAATAGTGAAAAAAGTTAAAGATAAAAAAAGAAAACTTTTTTTTTTTTGAGAAAAAAGAGTAAAGGTGAGAGGAAAAGATACCTTAAAAACAAATAAAGAATTAGAAAGAAAAAGAAAAATTATAGTGAGCAAAAATTTAGAGATAAAAGCATAATTCGAGGTATAATTGTTAGAGAAAATAAATAAATCTAAAGAGAAATTAAAGTTATATTGCAAGAAAATTGAACGAGAAAAGAAGATATAATTTTTAGAGGAAAAATAAATTAGAAAGAGAAATAAAAATTATAAAAGACAAATCCCTGAGAGAAAAAAATTCAGAAGAATATTAAAATTTTTAGAGGGAGAAATAAGAGTGATCCGTTAGAAAAAAAAAGAAAAAAATAGATAGAGATGAAAATTTTTGGAGAGTAAAAAATAATGATAAATATTAAAATATTAAAATTTTAGATTGAGAAAAATAAATTTTTTAGAGATATGTATAAGAAAAAATTTTAGAATGAGAAAAAAATTTGTTAAAGATAAATATGTCAAATAAAATAATTAAGTATTAGAAGAAAAAATAAGGTTTTAAAATTTTTTTCCTTTTTACATGGAATTTTTAAATGGTTTTTTTTAAATAAAAAATTGAATGGGTTGTCAAAAACTAAGAAACAAAAACAAAATTAATTGGTTCTTAAACGTGCCATAATTTTTTTTATTGGAAGGCGCATTATTGATATAAAATATATTACTAAATTAAATTCTTCTGATATTTAATAATAAAAATTCTATAATGTAAATTTCAGAAATTCTCGTAGACGATCCATTTACAACCAAAAAAATTTGAAATTTAAAAAAGATTAGGATAAAGACGAACCGATTAGAGATAAGATAAAAGAAATTTGAAAATGAAGTAGGAAATGGAAACCGAAATCCAATAGAATTTTGTTTCCCAGGAGATGGGAATAAGCTCCTCCCTCCTAACTCTTATATATAAATTTCTTAATGTCTCCTCATTTTGATCATTTGAAAACTCTCAAGTGAAGTATTTTGTTGTTATTGAATTTTATGTGAAAAATATAATTAATGGAGCATCTATGTGATCTTCTTGGAGATTCCACAATCAAAGTTAAAGCTGACTCCTCTTCTCAATTGCAATTGCAAGCTCCTCTTGATCCTAAATCAATATCACTGGTGGAAGAAATCAGGTTGGAACTCCAAGTCCAAACCGAATGCCATCACTTCCAAGTGGTCTCCATTGCTCCAACTTTGGTTAGCCATCACCATTCCCCTACATTCTCCATAACATCTTACCAACTTCCTGTGCACATCTTCAATCCTGGCATTCACGACAACACTCTGCAGCGTTTCCTATCATGGTAGTTCAAAGAATATGAACATTGCATTAACATACAAGTCATGACACACCAAATAATAAACCACTGGTTCCATGTCAAACGAGAAGCAATTGCAACTCGTGATCATAACAAGACTCAAATTCCATTGCGTTTGGGAGAGTTTTTTCTCTTGAAGTTACCCTACACTTGTGGAATTCGGTCATTGAAGATGATGATGATTATGCTATGGTTCCAGCAAGCGAAGTTGCTATTGAGAAGATGTTAAAGACCGTAAAGTTTGAGGAGATTGTATTAGAATCAGCAACTACTTGTGTGATTTGCTTGGAGGAGATCACCACGATGCCCAACCAAGATATCGATAGTGGAATGTTATTGGTGCAGATGCCTTGTTTACGTGTGTTTCATCAAAAAAGCATCCTCAAGTGGCTCAACAACAGTCATTATTGTCCCACCGGTAGATTTCAAATGCCTACAAATTTAACAAATTCCCAACATTTGGCTCACTATTCTGCATAG

mRNA sequence

ATGTTTTGCTTCGTCGATTACCAACTTCCAGAGTGCATCATCAACCGTGGCATTGGCGACAAGACTCTGCAGCGCTTCCTATCATGGCAGTTCAAAGAATATGAAGACTCCATTAACATACAAGTTATGACACACCAAATAATAAACCACTGGCTCCATGTCAAACGAGAAGGAGTTGCAGCAACAACTCAGGATCATAATGATAATTCCAATTCCAATGCGTTTGGGAGAGTTTTTCCCCTTGAAGTTACCCTGCATTTGTGGAATTCAGTCATTCATTATGATTATGATTATGCTATGGTTCCAGCAAGCGAAGTTGGTATTGAGAAGATGTTAAAGACCGTAAAGTTTGAGGAGATTGTACTGGAATCAGCAACTACTTGTGTCATTTGCTTGGAGGAGATCACCACGATGCCCAACCAAGATATCGACAGTGGAATGTTATTACTGCAAATACCTTGCTTGCATGTGTTTCATGGAAGATGCATCAAGAAGTGGCTTAACAACAGTCATTATTTTACCCTACACTTGTGGAATTCGGTCATTGAAGATGATGATGATTATGCTATGGTTCCAGCAAGCGAAGTTGCTATTGAGAAGATGTTAAAGACCGTAAAGTTTGAGGAGATTGTATTAGAATCAGCAACTACTTGTGTGATTTGCTTGGAGGAGATCACCACGATGCCCAACCAAGATATCGATAGTGGAATGTTATTGGTGCAGATGCCTTGTTTACGTGTGTTTCATCAAAAAAGCATCCTCAAGTGGCTCAACAACAGTCATTATTGTCCCACCGGTAGATTTCAAATGCCTACAAATTTAACAAATTCCCAACATTTGGCTCACTATTCTGCATAG

Coding sequence (CDS)

ATGTTTTGCTTCGTCGATTACCAACTTCCAGAGTGCATCATCAACCGTGGCATTGGCGACAAGACTCTGCAGCGCTTCCTATCATGGCAGTTCAAAGAATATGAAGACTCCATTAACATACAAGTTATGACACACCAAATAATAAACCACTGGCTCCATGTCAAACGAGAAGGAGTTGCAGCAACAACTCAGGATCATAATGATAATTCCAATTCCAATGCGTTTGGGAGAGTTTTTCCCCTTGAAGTTACCCTGCATTTGTGGAATTCAGTCATTCATTATGATTATGATTATGCTATGGTTCCAGCAAGCGAAGTTGGTATTGAGAAGATGTTAAAGACCGTAAAGTTTGAGGAGATTGTACTGGAATCAGCAACTACTTGTGTCATTTGCTTGGAGGAGATCACCACGATGCCCAACCAAGATATCGACAGTGGAATGTTATTACTGCAAATACCTTGCTTGCATGTGTTTCATGGAAGATGCATCAAGAAGTGGCTTAACAACAGTCATTATTTTACCCTACACTTGTGGAATTCGGTCATTGAAGATGATGATGATTATGCTATGGTTCCAGCAAGCGAAGTTGCTATTGAGAAGATGTTAAAGACCGTAAAGTTTGAGGAGATTGTATTAGAATCAGCAACTACTTGTGTGATTTGCTTGGAGGAGATCACCACGATGCCCAACCAAGATATCGATAGTGGAATGTTATTGGTGCAGATGCCTTGTTTACGTGTGTTTCATCAAAAAAGCATCCTCAAGTGGCTCAACAACAGTCATTATTGTCCCACCGGTAGATTTCAAATGCCTACAAATTTAACAAATTCCCAACATTTGGCTCACTATTCTGCATAG

Protein sequence

MFCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVAATTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDYAMVPASEVGIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHYFTLHLWNSVIEDDDDYAMVPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLVQMPCLRVFHQKSILKWLNNSHYCPTGRFQMPTNLTNSQHLAHYSA

Homology

[Close]

BLAST of Moc04g08220 vs. NCBI nr
Match: XP_022158635.1 (RING-H2 finger protein ATL47-like [Momordica charantia])

HSP 1 Score: 366.3 bits (939), Expect = 2.4e-97
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MFCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVA 60
           MFCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVA
Sbjct: 1   MFCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVA 60

Query: 61  ATTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDYAMVPASEVGIEKMLKTVKFEEI 120
           ATTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDYAMVPASEVGIEKMLKTVKFEEI
Sbjct: 61  ATTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDYAMVPASEVGIEKMLKTVKFEEI 120

Query: 121 VLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY 173
           VLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY
Sbjct: 121 VLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY 172

[Close]

BLAST of Moc04g08220 vs. NCBI nr
Match: XP_022158633.1 (E3 ubiquitin-protein ligase RING1-like [Momordica charantia])

HSP 1 Score: 232.3 bits (591), Expect = 5.4e-57
Identity = 133/238 (55.88%), Postives = 138/238 (57.98%), Query Frame = 0


Query: 43  MTHQIINHWLHVKRE-GVAATTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDYAMV 102
           MT QIINHWLHVKRE   AATT+D N+NSNS AFGRVFPLEVTLHLWNSVI         
Sbjct: 1   MTXQIINHWLHVKREAAAAATTRDRNNNSNSIAFGRVFPLEVTLHLWNSVIE-------- 60

Query: 103 PASEVGIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGR 162
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 163 CIKKWLNNSHYFTLHLWNSVIEDDDDYAMVPASEVAIEKMLKTVKFEEIVLESATTCVIC 222
                                +DDDDYAMVPASEV IEKMLKTVKFEEIVLESATTCVIC
Sbjct: 121 ---------------------DDDDDYAMVPASEVGIEKMLKTVKFEEIVLESATTCVIC 149

Query: 223 LEEITTMPNQDIDSGMLLVQMPCLRVFHQKSILKWLNNSHYCPTGRFQMPTNLTNSQH 280
           LEEITTMPNQDIDSGMLLVQMPCL VFH+K ILKWLNNSHYCPT RFQMPTNLTN+QH
Sbjct: 181 LEEITTMPNQDIDSGMLLVQMPCLHVFHKKCILKWLNNSHYCPTCRFQMPTNLTNAQH 149

[Close]

BLAST of Moc04g08220 vs. NCBI nr
Match: XP_022158634.1 (E3 ubiquitin-protein ligase RING1-like [Momordica charantia])

HSP 1 Score: 223.4 bits (568), Expect = 2.5e-54
Identity = 112/132 (84.85%), Postives = 117/132 (88.64%), Query Frame = 0


Query: 43  MTHQIINHWLHVKREGVAAT-TQDHNDNSNSNAFGRVFPLEVTLHLWNSVIH-YDYDYAM 102
           MTHQIINHWLHVKRE  AA+ T+DHN+NSNS AFGRVF LEVT HLWNSVI   D DYAM
Sbjct: 1   MTHQIINHWLHVKREAAAASATRDHNNNSNSIAFGRVFSLEVTFHLWNSVIEDDDDDYAM 60

Query: 103 VPASEVGIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHG 162
           VPASEV IEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDID+GMLL+ IPCLHVFH 
Sbjct: 61  VPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDNGMLLVHIPCLHVFHH 120

Query: 163 RCIKKWLNNSHY 173
            CI KWLNNSHY
Sbjct: 121 ICILKWLNNSHY 132

[Close]

BLAST of Moc04g08220 vs. NCBI nr
Match: KAE8645828.1 (hypothetical protein Csa_017274 [Cucumis sativus])

HSP 1 Score: 125.9 bits (315), Expect = 5.4e-25
Identity = 116/446 (26.01%), Postives = 163/446 (36.55%), Query Frame = 0


Query: 8   QLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVAATTQDHN 67
           QLP  I     GDKTL+R L  +F+ Y   INI+++  +II HW+    E          
Sbjct: 92  QLPVSIFKH--GDKTLKRLLFREFQIYRAIINIELVVDEIIKHWVKKVDE---------- 151

Query: 68  DNSNSNAFGRVFPLEVTLHLW-----------NSVIHYDYDYAMVPASEVGIEKMLKTVK 127
           +NS S  F  ++PLE+T+ L            + ++       MVP SE  +E MLK V+
Sbjct: 152 ENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQVMMVPTSESAMESMLKKVE 211

Query: 128 FEEIVL---ESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY- 187
            EEIV      +  CVICLEEI+    +   S  ++LQ+PCLH+FH  CI+KWL  SHY 
Sbjct: 212 NEEIVKFGDNKSINCVICLEEIS---KEKKGSEAVVLQMPCLHMFHEECIRKWLKRSHYC 271

Query: 188 ------------------------------------------------------------ 247
                                                                       
Sbjct: 272 PTFFIFISTSMAEQNFDDCTSGYYMGMSLRITDPIQTRSNSPTSVKQIDLNFKVEVKCQH 331

Query: 248 --------------FTLHL----------------------------------------- 274
                          + H+                                         
Sbjct: 332 FQAGEEDTTFATTFLSEHIIAHQPFPALQLPVSIFKHGDKTLKRLLFQKFQMFRAIINIE 391

[Close]

BLAST of Moc04g08220 vs. NCBI nr
Match: ADN34252.1 (hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putative zinc finger protein [Cucumis melo var. makuwa] >TYK06863.1 putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 117.1 bits (292), Expect = 2.5e-22
Identity = 67/180 (37.22%), Postives = 102/180 (56.67%), Query Frame = 0


Query: 4   FVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVAATT 63
           F  +QLP  I     GDKTL+R L  +FK Y D INI+++ ++II HW+    E      
Sbjct: 85  FATFQLPISIFKH--GDKTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEE------ 144

Query: 64  QDHNDNSNSNAFGRVFPLEVTLHLWNSV--------IHYDYDYAMVPASEVGIEKMLKTV 123
           ++  +N+++  F +++PLE+ + L  S         +       MVP S+  +E MLK V
Sbjct: 145 EEDQENNSNVLFKKIYPLEIMIRLLVSKMIRAVQPRLAMIDQPLMVPTSDSAVESMLKRV 204

Query: 124 KFEEIVL---ESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY 173
           + EEI+    + +  CV+CLEEI+    ++  S   +LQ+PCLH+FH  CI+KWL  SHY
Sbjct: 205 ENEEIMKSGDDESINCVVCLEEIS---KEEKGSETTVLQMPCLHMFHEECIRKWLKTSHY 253

[Close]

BLAST of Moc04g08220 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.1e-07
Identity = 34/91 (37.36%), Postives = 50/91 (54.95%), Query Frame = 0


Query: 184 DDDDYAMVPASEVAIEKMLKTVKFEEIVLES-ATTCVICLEEITTMPNQDIDSGMLLVQM 243
           D + Y   PAS+ AI+  L TVK  + +L+S    C +C++E         + G  + QM
Sbjct: 182 DPNRYGTPPASKSAID-ALPTVKVTKDMLKSEMNQCAVCMDE--------FEDGSDVKQM 241

Query: 244 PCLRVFHQKSILKWLNNSHYCPTGRFQMPTN 274
           PC  VFHQ  +L WL   + CP  RF++PT+
Sbjct: 242 PCKHVFHQDCLLPWLELHNSCPVCRFELPTD 263

[Close]

BLAST of Moc04g08220 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 7.6e-06
Identity = 32/84 (38.10%), Postives = 43/84 (51.19%), Query Frame = 0


Query: 192 PASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLVQMPCLRVFHQK 251
           PA + A+E  L TVK EE+V     +C +CL+        D++ G    QMPC   FH  
Sbjct: 179 PAKKEAVE-ALPTVKIEEVV-----SCSVCLD--------DLEVGSQAKQMPCEHKFHSS 238

Query: 252 SILKWLNNSHYCPTGRFQMPTNLT 276
            IL WL     CP  RF++P+  T
Sbjct: 239 CILPWLELHSSCPVCRFELPSEET 248

[Close]

BLAST of Moc04g08220 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.7e-05
Identity = 32/92 (34.78%), Postives = 47/92 (51.09%), Query Frame = 0


Query: 184 DDDDYAMVPASEVAIEKMLKTVKFEEIVLESA--TTCVICLEEITTMPNQDIDSGMLLVQ 243
           D  +   VPA+  A+   L+ V+   +   +A    CVIC EE++         G  + +
Sbjct: 179 DQKEGYQVPAARAAV-VALRAVEVFNVAASNAGEVECVICKEEMS--------EGRDVCE 238

Query: 244 MPCLRVFHQKSILKWLNNSHYCPTGRFQMPTN 274
           MPC   FH K IL WL+  + CP  RFQ+PT+
Sbjct: 239 MPCQHFFHWKCILPWLSKKNTCPFCRFQLPTD 261

[Close]

BLAST of Moc04g08220 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.2e-04
Identity = 26/93 (27.96%), Postives = 47/93 (50.54%), Query Frame = 0


Query: 181 VIEDDDDYAMVPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLV 240
           ++ D D +   PA++ A+E + +TV       ++   C +CL E         +     +
Sbjct: 42  LVVDWDHHLPPPAAKTAVENLPRTVIRGS---QAELKCPVCLLE--------FEEEETAI 101

Query: 241 QMPCLRVFHQKSILKWLNNSHYCPTGRFQMPTN 274
           +MPC  +FH   IL WL+ ++ CP  R ++PT+
Sbjct: 102 EMPCHHLFHSNCILPWLSKTNSCPLCRHELPTD 123

[Close]

BLAST of Moc04g08220 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.2e-04
Identity = 25/93 (26.88%), Postives = 47/93 (50.54%), Query Frame = 0


Query: 181 VIEDDDDYAMVPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLV 240
           ++ D D +   PA++  +E + +TV       ++   C +CL E         +     +
Sbjct: 42  LVVDWDHHLPPPAAKTVVENLPRTVIRGS---QAELKCPVCLLE--------FEEEETAI 101

Query: 241 QMPCLRVFHQKSILKWLNNSHYCPTGRFQMPTN 274
           +MPC  +FH   IL WL+ ++ CP  R+++PT+
Sbjct: 102 EMPCHHLFHSSCILPWLSKTNSCPLCRYELPTD 123

[Close]

BLAST of Moc04g08220 vs. ExPASy TrEMBL
Match: A0A6J1DWD8 (RING-H2 finger protein ATL47-like OS=Momordica charantia OX=3673 GN=LOC111025085 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.2e-97
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MFCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVA 60
           MFCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVA
Sbjct: 1   MFCFVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVA 60

Query: 61  ATTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDYAMVPASEVGIEKMLKTVKFEEI 120
           ATTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDYAMVPASEVGIEKMLKTVKFEEI
Sbjct: 61  ATTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDYAMVPASEVGIEKMLKTVKFEEI 120

Query: 121 VLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY 173
           VLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY
Sbjct: 121 VLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY 172

[Close]

BLAST of Moc04g08220 vs. ExPASy TrEMBL
Match: A0A6J1DWN3 (E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC111025082 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 2.6e-57
Identity = 133/238 (55.88%), Postives = 138/238 (57.98%), Query Frame = 0


Query: 43  MTHQIINHWLHVKRE-GVAATTQDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDYAMV 102
           MT QIINHWLHVKRE   AATT+D N+NSNS AFGRVFPLEVTLHLWNSVI         
Sbjct: 1   MTXQIINHWLHVKREAAAAATTRDRNNNSNSIAFGRVFPLEVTLHLWNSVIE-------- 60

Query: 103 PASEVGIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGR 162
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 163 CIKKWLNNSHYFTLHLWNSVIEDDDDYAMVPASEVAIEKMLKTVKFEEIVLESATTCVIC 222
                                +DDDDYAMVPASEV IEKMLKTVKFEEIVLESATTCVIC
Sbjct: 121 ---------------------DDDDDYAMVPASEVGIEKMLKTVKFEEIVLESATTCVIC 149

Query: 223 LEEITTMPNQDIDSGMLLVQMPCLRVFHQKSILKWLNNSHYCPTGRFQMPTNLTNSQH 280
           LEEITTMPNQDIDSGMLLVQMPCL VFH+K ILKWLNNSHYCPT RFQMPTNLTN+QH
Sbjct: 181 LEEITTMPNQDIDSGMLLVQMPCLHVFHKKCILKWLNNSHYCPTCRFQMPTNLTNAQH 149

[Close]

BLAST of Moc04g08220 vs. ExPASy TrEMBL
Match: A0A6J1DZZ7 (E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC111025083 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.2e-54
Identity = 112/132 (84.85%), Postives = 117/132 (88.64%), Query Frame = 0


Query: 43  MTHQIINHWLHVKREGVAAT-TQDHNDNSNSNAFGRVFPLEVTLHLWNSVIH-YDYDYAM 102
           MTHQIINHWLHVKRE  AA+ T+DHN+NSNS AFGRVF LEVT HLWNSVI   D DYAM
Sbjct: 1   MTHQIINHWLHVKREAAAASATRDHNNNSNSIAFGRVFSLEVTFHLWNSVIEDDDDDYAM 60

Query: 103 VPASEVGIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHG 162
           VPASEV IEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDID+GMLL+ IPCLHVFH 
Sbjct: 61  VPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDNGMLLVHIPCLHVFHH 120

Query: 163 RCIKKWLNNSHY 173
            CI KWLNNSHY
Sbjct: 121 ICILKWLNNSHY 132

[Close]

BLAST of Moc04g08220 vs. ExPASy TrEMBL
Match: A0A0A0K2A5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G068580 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 5.5e-23
Identity = 71/177 (40.11%), Postives = 100/177 (56.50%), Query Frame = 0


Query: 4   FVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVAATT 63
           F  ++LP  I+    GDKTL+  L  +F  Y D INI+ +  +II +W+      V    
Sbjct: 85  FATFRLPISILEH--GDKTLKLLLFREFHMYRDIINIEHLVDEIIKYWV----TKVEEEE 144

Query: 64  QDHNDNSNSNAFGRVFPLEVTLHLWNSVIHYDYDY-----AMVPASEVGIEKMLKTVKFE 123
           +D  ++++S  F +++ LE+TL L    I    DY      MVP S+  +E MLK V+ E
Sbjct: 145 EDQENSTSSRVFKKIYRLEITLELLIFQIVRVMDYQPQVLMMVPTSDSAMESMLKRVENE 204

Query: 124 EIVL---ESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY 173
           EI+    +    CVICLEEI     ++  S  ++LQ+PCLHVFHG CI KWLN SHY
Sbjct: 205 EIMKLGDDDCIHCVICLEEI---GKEEKGSEGVVLQMPCLHVFHGECINKWLNTSHY 252

[Close]

BLAST of Moc04g08220 vs. ExPASy TrEMBL
Match: A0A5D3C4K6 (Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001670 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.2e-22
Identity = 67/180 (37.22%), Postives = 102/180 (56.67%), Query Frame = 0


Query: 4   FVDYQLPECIINRGIGDKTLQRFLSWQFKEYEDSINIQVMTHQIINHWLHVKREGVAATT 63
           F  +QLP  I     GDKTL+R L  +FK Y D INI+++ ++II HW+    E      
Sbjct: 85  FATFQLPISIFKH--GDKTLKRILFREFKMYGDIINIELLANEIIKHWVRKVEE------ 144

Query: 64  QDHNDNSNSNAFGRVFPLEVTLHLWNSV--------IHYDYDYAMVPASEVGIEKMLKTV 123
           ++  +N+++  F +++PLE+ + L  S         +       MVP S+  +E MLK V
Sbjct: 145 EEDQENNSNVLFKKIYPLEIMIRLLVSKMIRAVQPRLAMIDQPLMVPTSDSAVESMLKRV 204

Query: 124 KFEEIVL---ESATTCVICLEEITTMPNQDIDSGMLLLQIPCLHVFHGRCIKKWLNNSHY 173
           + EEI+    + +  CV+CLEEI+    ++  S   +LQ+PCLH+FH  CI+KWL  SHY
Sbjct: 205 ENEEIMKSGDDESINCVVCLEEIS---KEEKGSETTVLQMPCLHMFHEECIRKWLKTSHY 253

[Close]

BLAST of Moc04g08220 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 2.2e-08
Identity = 34/91 (37.36%), Postives = 50/91 (54.95%), Query Frame = 0


Query: 184 DDDDYAMVPASEVAIEKMLKTVKFEEIVLES-ATTCVICLEEITTMPNQDIDSGMLLVQM 243
           D + Y   PAS+ AI+  L TVK  + +L+S    C +C++E         + G  + QM
Sbjct: 182 DPNRYGTPPASKSAID-ALPTVKVTKDMLKSEMNQCAVCMDE--------FEDGSDVKQM 241

Query: 244 PCLRVFHQKSILKWLNNSHYCPTGRFQMPTN 274
           PC  VFHQ  +L WL   + CP  RF++PT+
Sbjct: 242 PCKHVFHQDCLLPWLELHNSCPVCRFELPTD 263

[Close]

BLAST of Moc04g08220 vs. TAIR 10
Match: AT5G02750.1 (RING/U-box superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 1.2e-06
Identity = 32/92 (34.78%), Postives = 47/92 (51.09%), Query Frame = 0


Query: 184 DDDDYAMVPASEVAIEKMLKTVKFEEIVLESA--TTCVICLEEITTMPNQDIDSGMLLVQ 243
           D  +   VPA+  A+   L+ V+   +   +A    CVIC EE++         G  + +
Sbjct: 179 DQKEGYQVPAARAAV-VALRAVEVFNVAASNAGEVECVICKEEMS--------EGRDVCE 238

Query: 244 MPCLRVFHQKSILKWLNNSHYCPTGRFQMPTN 274
           MPC   FH K IL WL+  + CP  RFQ+PT+
Sbjct: 239 MPCQHFFHWKCILPWLSKKNTCPFCRFQLPTD 261

[Close]

BLAST of Moc04g08220 vs. TAIR 10
Match: AT3G13228.1 (RING/U-box superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 4.6e-06
Identity = 24/89 (26.97%), Postives = 48/89 (53.93%), Query Frame = 0


Query: 183 EDDDDYAMVPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLVQM 242
           ++  ++ + PAS++ ++ +  T K  + +  +   C ICLEE         ++G +LV +
Sbjct: 242 DETTNFCLGPASKLVVKSL--TRKIYDKINYTGERCTICLEE--------FNAGGILVAL 301

Query: 243 PCLRVFHQKSILKWLNNSHYCPTGRFQMP 272
           PC   F  +  +KW   +H+CP  R+++P
Sbjct: 302 PCGHDFDDECAVKWFETNHFCPLCRYELP 320

[Close]

BLAST of Moc04g08220 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 2.3e-05
Identity = 32/101 (31.68%), Postives = 44/101 (43.56%), Query Frame = 0


Query: 173 FTLHLWNSVIEDDDDYAMVPASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQD 232
           F + L      D + Y   PA + A+E  L TVK EE +      C +CL+        D
Sbjct: 184 FEMLLQRLAENDPNRYGTPPAKKEAVE-ALATVKIEETL-----QCSVCLD--------D 243

Query: 233 IDSGMLLVQMPCLRVFHQKSILKWLNNSHYCPTGRFQMPTN 274
            + G     MPC   FH   +L WL     CP  R+Q+P +
Sbjct: 244 FEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPAD 270

[Close]

BLAST of Moc04g08220 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 45.4 bits (106), Expect = 8.7e-05
Identity = 25/82 (30.49%), Postives = 43/82 (52.44%), Query Frame = 0


Query: 192 PASEVAIEKMLKTVKFEEIVLESATTCVICLEEITTMPNQDIDSGMLLVQMPCLRVFHQK 251
           PAS+ AIE  L  V+  +   ++   C +C E          ++G+   +MPC  +FH  
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAEANCAVCTEV--------FEAGIEGREMPCKHIFHGD 249

Query: 252 SILKWLNNSHYCPTGRFQMPTN 274
            I+ WL+  + CP  RF++P++
Sbjct: 250 CIVPWLSIRNSCPVCRFELPSD 262

The following BLAST results are available for this feature:

BLAST of Moc04g08220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158635.1	2.4e-97	100.00	RING-H2 finger protein ATL47-like [Momordica charantia]	[more]
XP_022158633.1	5.4e-57	55.88	E3 ubiquitin-protein ligase RING1-like [Momordica charantia]	[more]
XP_022158634.1	2.5e-54	84.85	E3 ubiquitin-protein ligase RING1-like [Momordica charantia]	[more]
KAE8645828.1	5.4e-25	26.01	hypothetical protein Csa_017274 [Cucumis sativus]	[more]
ADN34252.1	2.5e-22	37.22	hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putati...	[more]

BLAST of Moc04g08220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	3.1e-07	37.36	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q6AVN2	7.6e-06	38.10	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8GXF8	1.7e-05	34.78	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
Q3T0W3	3.2e-04	27.96	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]
Q9P0P0	3.2e-04	26.88	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]

BLAST of Moc04g08220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWD8	1.2e-97	100.00	RING-H2 finger protein ATL47-like OS=Momordica charantia OX=3673 GN=LOC111025085...	[more]
A0A6J1DWN3	2.6e-57	55.88	E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1DZZ7	1.2e-54	84.85	E3 ubiquitin-protein ligase RING1-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0K2A5	5.5e-23	40.11	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G068580 P...	[more]
A0A5D3C4K6	1.2e-22	37.22	Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of Moc04g08220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	2.2e-08	37.36	RING/U-box superfamily protein	[more]
AT5G02750.1	1.2e-06	34.78	RING/U-box superfamily protein	[more]
AT3G13228.1	4.6e-06	26.97	RING/U-box superfamily protein	[more]
AT1G55530.1	2.3e-05	31.68	RING/U-box superfamily protein	[more]
AT3G46620.1	8.7e-05	30.49	zinc finger (C3HC4-type RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 128..184 e-value: 0.17 score: 17.9 coord: 218..266 e-value: 0.23 score: 16.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 217..265 e-value: 9.5E-7 score: 29.1 coord: 127..170 e-value: 3.9E-7 score: 30.3
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 181..274 e-value: 6.8E-12 score: 46.9 coord: 94..173 e-value: 2.0E-10 score: 42.2
None	No IPR available	PANTHER	PTHR15710:SF63	RING/U-BOX SUPERFAMILY PROTEIN	coord: 182..272
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 182..272
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 48..172
None	No IPR available	PANTHER	PTHR15710:SF63	RING/U-BOX SUPERFAMILY PROTEIN	coord: 48..172
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 202..271
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 113..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g08220.1	Moc04g08220.1	mRNA