Homology
BLAST of Moc04g08140 vs. NCBI nr
Match:
XP_022158631.1 (squalene monooxygenase-like [Momordica charantia])
HSP 1 Score: 1019.6 bits (2635), Expect = 1.1e-293
Identity = 507/510 (99.41%), Postives = 507/510 (99.41%), Query Frame = 0
Query: 88 FRARRFNNGISNKTNTDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAAL 147
F RFNNGISNKTNTDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAAL
Sbjct: 23 FAHLRFNNGISNKTNTDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAAL 82
Query: 148 AYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALY 207
AYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALY
Sbjct: 83 AYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALY 142
Query: 208 KDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTV 267
KDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTV
Sbjct: 143 KDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTV 202
Query: 268 KGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENH 327
KGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENH
Sbjct: 203 KGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENH 262
Query: 328 GHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASF 387
GHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASF
Sbjct: 263 GHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASF 322
Query: 388 IAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLR 447
IAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLR
Sbjct: 323 IAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLR 382
Query: 448 PLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLS 507
PLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLS
Sbjct: 383 PLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLS 442
Query: 508 LGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAG 567
LGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAG
Sbjct: 443 LGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAG 502
Query: 568 IIFPIIKAEGVRQMFFPATVPAYYRAPPMN 598
IIFPIIKAEGVRQMFFPATVPAYYRAPPMN
Sbjct: 503 IIFPIIKAEGVRQMFFPATVPAYYRAPPMN 532
BLAST of Moc04g08140 vs. NCBI nr
Match:
XP_038887697.1 (squalene monooxygenase SE2-like [Benincasa hispida])
HSP 1 Score: 884.4 bits (2284), Expect = 5.4e-253
Identity = 436/504 (86.51%), Postives = 466/504 (92.46%), Query Frame = 0
Query: 93 FNNGISNKTNTDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAALAYTLA 152
F +GI+N + K + +AV P + S++PDV++VGAGVAGAALA+TLA
Sbjct: 25 FRDGINNAAAAPAVDSK------STNSAVQIPLCSETSDDPDVIVVGAGVAGAALAFTLA 84
Query: 153 KDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALYKDGKH 212
KDGR+VHVIERDL EP+RIVGELLQPGGYLKLIELGLEDS+ GIDAQQVFGYALYKDG H
Sbjct: 85 KDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDSLKGIDAQQVFGYALYKDGNH 144
Query: 213 TKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTVKGVQY 272
T+LAYPL+ FDS+VSGRSFHNGRFIQRLR+ AA+LSNVRLEQGTVTS+VEEDG VKGVQY
Sbjct: 145 TRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVRLEQGTVTSVVEEDGIVKGVQY 204
Query: 273 KTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENHGHVVL 332
KTKNG+ELTAYAPLTIVCDGGFSNLRR LCKPQIDIPSCFVGLVLENCKLP ENHGHVVL
Sbjct: 205 KTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSCFVGLVLENCKLPLENHGHVVL 264
Query: 333 ADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASFIAAIE 392
ADPSPILLYPISSTE+RCLVDIPGQKVPSVANGEMA YLKTVVAPQ+PPEL+A+FIAAIE
Sbjct: 265 ADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAQYLKTVVAPQLPPELYAAFIAAIE 324
Query: 393 KGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPLANL 452
KGNIKSTTNR+MPA P PTPGALLLGDAFNMRHPLTGGGMTVALADIV+LRDLLRPL+NL
Sbjct: 325 KGNIKSTTNRSMPAIPHPTPGALLLGDAFNMRHPLTGGGMTVALADIVLLRDLLRPLSNL 384
Query: 453 KDADALCNYFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGLF 512
KDADALCNY ESFYTLRKPVASTINTLAGALY+VFCASPDPARKEMREACFDYLSLGG+F
Sbjct: 385 KDADALCNYLESFYTLRKPVASTINTLAGALYRVFCASPDPARKEMREACFDYLSLGGVF 444
Query: 513 SSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAGIIFPI 572
SSGPVALLSGLNPRPLSLFCHFFAVAIYGV RLLIPFPSP RIWIGVRLITGAAGIIFPI
Sbjct: 445 SSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPSPKRIWIGVRLITGAAGIIFPI 504
Query: 573 IKAEGVRQMFFPATVPAYYRAPPM 597
IKAEGVRQMFFPATVPAYYR PPM
Sbjct: 505 IKAEGVRQMFFPATVPAYYRTPPM 522
BLAST of Moc04g08140 vs. NCBI nr
Match:
XP_022952179.1 (squalene epoxidase 1-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 879.0 bits (2270), Expect = 2.3e-251
Identity = 427/467 (91.43%), Postives = 453/467 (97.00%), Query Frame = 0
Query: 130 SNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGL 189
S++ DV++VGAGVAGAALAYTLAKDGRKVHVIERDL+EPERIVGELLQPGGYLKLIELGL
Sbjct: 58 SDDLDVIVVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGL 117
Query: 190 EDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSN 249
EDS+ GIDAQQVFGYAL+KDG HT+LAYPL FDS++SGRSFHNGRFIQRLRQ AA+LSN
Sbjct: 118 EDSLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSN 177
Query: 250 VRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIP 309
V+LEQGTVTSIVEEDGTVKGVQYKTKNGQELT+YAPLTIVCDGGFSNLRRNLCKPQIDIP
Sbjct: 178 VKLEQGTVTSIVEEDGTVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIP 237
Query: 310 SCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAH 369
SCFVGLVLENCKLP ENHGHVVLADPSPILLY ISSTEIRCLVDIPGQKVPSVANG+MAH
Sbjct: 238 SCFVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAH 297
Query: 370 YLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTG 429
YLKTVVAPQ+PPEL +FIA+++KGNIKSTTNR+MPAAP PTPGALLLGDAFNMRHPLTG
Sbjct: 298 YLKTVVAPQLPPELQTAFIASVDKGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTG 357
Query: 430 GGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCA 489
GGMTVALADIVVLRDLLRPL+NL DADALCNY ESFYTLRKPVASTINTLAGALY+VFCA
Sbjct: 358 GGMTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCA 417
Query: 490 SPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPF 549
SPDPARKEMREACFDYLSLGG+FSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV+RLLIPF
Sbjct: 418 SPDPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPF 477
Query: 550 PSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRAPPM 597
PSP RIWIGVRLITGAAGIIFPIIKAEGVRQMFFPAT+PAYYRAPPM
Sbjct: 478 PSPKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 524
BLAST of Moc04g08140 vs. NCBI nr
Match:
KAG6571900.1 (Squalene monooxygenase SE2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 878.6 bits (2269), Expect = 3.0e-251
Identity = 428/467 (91.65%), Postives = 453/467 (97.00%), Query Frame = 0
Query: 130 SNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGL 189
S++ DV++VGAGVAGAALAYTLAKDGRKVHVIERDL+EPERIVGELLQPGGYLKLIELGL
Sbjct: 58 SDDLDVIVVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGL 117
Query: 190 EDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSN 249
EDS+ GIDAQQVFGYAL+KDG HT+LAYPL FDS++SGRSFHNGRFIQRLRQ AA+LSN
Sbjct: 118 EDSLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSN 177
Query: 250 VRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIP 309
V+LEQGTVTSIVEEDGTVKGVQYKTKNGQELT+YAPLTIVCDGGFSNLRRNLCKPQIDIP
Sbjct: 178 VKLEQGTVTSIVEEDGTVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIP 237
Query: 310 SCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAH 369
SCFVGLVLENCKLP ENHGHVVLADPSPILLY ISSTEIRCLVDIPGQKVPSVANG+MA
Sbjct: 238 SCFVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAR 297
Query: 370 YLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTG 429
YLKTVVAPQ+PPEL A+FIA++EKGNIKSTTNR+MPAAP PTPGALLLGDAFNMRHPLTG
Sbjct: 298 YLKTVVAPQLPPELQAAFIASVEKGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTG 357
Query: 430 GGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCA 489
GGMTVALADIVVLRDLLRPL+NL DADALCNY ESFYTLRKPVASTINTLAGALY+VFCA
Sbjct: 358 GGMTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCA 417
Query: 490 SPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPF 549
SPDPARKEMREACFDYLSLGG+FSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV+RLLIPF
Sbjct: 418 SPDPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPF 477
Query: 550 PSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRAPPM 597
PSP RIWIGVRLITGAAGIIFPIIKAEGVRQMFFPAT+PAYYRAPPM
Sbjct: 478 PSPKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 524
BLAST of Moc04g08140 vs. NCBI nr
Match:
KAG7011588.1 (Squalene epoxidase 1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 878.6 bits (2269), Expect = 3.0e-251
Identity = 428/467 (91.65%), Postives = 453/467 (97.00%), Query Frame = 0
Query: 130 SNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGL 189
S++ DV++VGAGVAGAALAYTLAKDGRKVHVIERDL+EPERIVGELLQPGGYLKLIELGL
Sbjct: 58 SDDLDVIVVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGL 117
Query: 190 EDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSN 249
EDS+ GIDAQQVFGYAL+KDG HT+LAYPL FDS++SGRSFHNGRFIQRLRQ AA+LSN
Sbjct: 118 EDSLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSN 177
Query: 250 VRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIP 309
V+LEQGTVTSIVEEDGTVKGVQYKTKNGQELT+YAPLTIVCDGGFSNLRRNLCKPQIDIP
Sbjct: 178 VKLEQGTVTSIVEEDGTVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIP 237
Query: 310 SCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAH 369
SCFVGLVLENCKLP ENHGHVVLADPSPILLY ISSTEIRCLVDIPGQKVPSVANG+MA
Sbjct: 238 SCFVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAR 297
Query: 370 YLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTG 429
YLKTVVAPQ+PPEL A+FIA++EKGNIKSTTNR+MPAAP PTPGALLLGDAFNMRHPLTG
Sbjct: 298 YLKTVVAPQLPPELQAAFIASVEKGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTG 357
Query: 430 GGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCA 489
GGMTVALADIVVLRDLLRPL+NL DADALCNY ESFYTLRKPVASTINTLAGALY+VFCA
Sbjct: 358 GGMTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCA 417
Query: 490 SPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPF 549
SPDPARKEMREACFDYLSLGG+FSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV+RLLIPF
Sbjct: 418 SPDPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPF 477
Query: 550 PSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRAPPM 597
PSP RIWIGVRLITGAAGIIFPIIKAEGVRQMFFPAT+PAYYRAPPM
Sbjct: 478 PSPKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 524
BLAST of Moc04g08140 vs. ExPASy Swiss-Prot
Match:
B7TWW5 (Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2)
HSP 1 Score: 778.1 bits (2008), Expect = 7.2e-224
Identity = 372/472 (78.81%), Postives = 426/472 (90.25%), Query Frame = 0
Query: 126 ENVQSNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLI 185
E++ + DV+IVGAGVAG+ALAYTLAKDGR+VHVIERDL E +RIVGELLQPGGYLKL+
Sbjct: 70 EDIAGSSDDVIIVGAGVAGSALAYTLAKDGRRVHVIERDLTEQDRIVGELLQPGGYLKLV 129
Query: 186 ELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAA 245
ELGLED VN IDAQ+VFGYALY DGK+T+L+YPL+ F + V+GRSFHNGRFIQR+R+ AA
Sbjct: 130 ELGLEDCVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHADVAGRSFHNGRFIQRMREKAA 189
Query: 246 SLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQELT-AYAPLTIVCDGGFSNLRRNLCKP 305
SL NVR+EQGTVTS+VE+ GTVKGV+YKTKNGQE++ AYAPLTIVCDG FSNLR +LC P
Sbjct: 190 SLPNVRMEQGTVTSLVEQKGTVKGVRYKTKNGQEMSAAYAPLTIVCDGCFSNLRHSLCNP 249
Query: 306 QIDIPSCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVAN 365
++D+PSCFVGL+LEN LP NHGHV+LADPSPIL Y ISSTEIRCLVD+PGQKVPS+AN
Sbjct: 250 KVDVPSCFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPSIAN 309
Query: 366 GEMAHYLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMR 425
GE+AHYLKT VAPQ+PPEL+ SFIAAI+KG IK+ NR+MPA P TPGALLLGDAFNMR
Sbjct: 310 GELAHYLKTSVAPQIPPELYKSFIAAIDKGKIKTMPNRSMPADPHSTPGALLLGDAFNMR 369
Query: 426 HPLTGGGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLAGALY 485
HPLTGGGMTVAL+DIV++RDLLRPL +L D+ LC Y ESFYTLRKPVASTINTLAGALY
Sbjct: 370 HPLTGGGMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALY 429
Query: 486 KVFCASPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSR 545
KVFCASPD AR+EMR+ACFDYLSLGG+ S GP+ALLSGLNPRP+SLF HFFAVAIYGV R
Sbjct: 430 KVFCASPDKARQEMRDACFDYLSLGGICSEGPIALLSGLNPRPMSLFFHFFAVAIYGVGR 489
Query: 546 LLIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRAPPM 597
LLIPFPSP ++W+G RLI+GA+GIIFPIIK+EGVRQMFFPATVPAYYRAPP+
Sbjct: 490 LLIPFPSPRKMWLGARLISGASGIIFPIIKSEGVRQMFFPATVPAYYRAPPI 541
BLAST of Moc04g08140 vs. ExPASy Swiss-Prot
Match:
O48651 (Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1)
HSP 1 Score: 770.0 bits (1987), Expect = 2.0e-221
Identity = 368/472 (77.97%), Postives = 425/472 (90.04%), Query Frame = 0
Query: 126 ENVQSNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLI 185
EN+ + DV+IVGAGVAG+ALAYTLA DGR+VHVIERDL E +RIVGELLQPGGYLKLI
Sbjct: 69 ENI-AGSTDVIIVGAGVAGSALAYTLANDGRRVHVIERDLTEQDRIVGELLQPGGYLKLI 128
Query: 186 ELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAA 245
ELGLED VN IDAQ+VFGYALY DGK+T+L+YPL+ F S V+GRSFHNGRF+QR+R+ AA
Sbjct: 129 ELGLEDCVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHSDVAGRSFHNGRFVQRMREKAA 188
Query: 246 SLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQ 305
SL NVR+EQGTVTS+VE+ G+VKGVQYKTK+GQEL+A+APLTIVCDG FSNLRR+LC P+
Sbjct: 189 SLPNVRMEQGTVTSLVEKKGSVKGVQYKTKDGQELSAFAPLTIVCDGCFSNLRRSLCNPK 248
Query: 306 IDIPSCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANG 365
+++PSCFVGL+LEN LP NHGHV+LADPSPIL Y ISSTEIRCLVD+PGQKVP ++NG
Sbjct: 249 VEVPSCFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPCISNG 308
Query: 366 EMAHYLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRH 425
E+A+YLKTVVAPQVP +L+ SFIAA++KGNI++ NR+MPA P PTPGALLLGDAFNMRH
Sbjct: 309 ELANYLKTVVAPQVPKQLYNSFIAAVDKGNIRTMPNRSMPADPHPTPGALLLGDAFNMRH 368
Query: 426 PLTGGGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLAGALYK 485
PLTGGGMTVAL+DIV++RDLLRPL +L D+ LC Y ESFYTLRKPVASTINTLAGALYK
Sbjct: 369 PLTGGGMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYK 428
Query: 486 VFCASPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRL 545
VFCASPD AR+EMR ACFDYLSLGG+ S GP+ALLSGLNPRP+SLF HFFAVAIYGV RL
Sbjct: 429 VFCASPDKARQEMRNACFDYLSLGGICSQGPIALLSGLNPRPISLFLHFFAVAIYGVGRL 488
Query: 546 LIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRAPPMN 598
LIPFPSP R+W+G RLI GA+GIIFPIIK+EG+RQMFFPA VPAYYRAPP++
Sbjct: 489 LIPFPSPKRMWLGARLILGASGIIFPIIKSEGLRQMFFPAIVPAYYRAPPIH 539
BLAST of Moc04g08140 vs. ExPASy Swiss-Prot
Match:
Q9SM02 (Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1)
HSP 1 Score: 752.3 bits (1941), Expect = 4.2e-216
Identity = 363/495 (73.33%), Postives = 427/495 (86.26%), Query Frame = 0
Query: 107 IKKKKIFIAGSRAAVNPPSENVQS------NEPDVVIVGAGVAGAALAYTLAKDGRKVHV 166
+K K+ + R V+ + +V S DV++VGAGVAG+ALAYTL KD R+VHV
Sbjct: 29 VKPKRNGLRHDRKTVSTVTSDVGSVNITGDTVADVIVVGAGVAGSALAYTLGKDKRRVHV 88
Query: 167 IERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPLQ 226
IERDL+EP+RIVGELLQPGGYLKL+ELG+ED V IDAQ+V+GYAL+K+GK +LAYPL+
Sbjct: 89 IERDLSEPDRIVGELLQPGGYLKLLELGIEDCVEEIDAQRVYGYALFKNGKRIRLAYPLE 148
Query: 227 NFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQEL 286
F VSGRSFHNGRFIQR+R+ AASL NV+LEQGTV S++EE+GT+KGV+YK K G+E
Sbjct: 149 KFHEDVSGRSFHNGRFIQRMREKAASLPNVQLEQGTVLSLLEENGTIKGVRYKNKAGEEQ 208
Query: 287 TAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENHGHVVLADPSPILL 346
TA+A LTIVCDG FSNLRR+LC PQ+++PSCFVGLVLENC LP+ NHGHVVLADPSPIL+
Sbjct: 209 TAFAALTIVCDGCFSNLRRSLCNPQVEVPSCFVGLVLENCNLPYANHGHVVLADPSPILM 268
Query: 347 YPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASFIAAIEKGNIKSTT 406
YPISSTE+RCLVD+PGQKVPS+ANGEM +YLKTVVAPQ+P E++ SFIAA++KGNIKS
Sbjct: 269 YPISSTEVRCLVDVPGQKVPSIANGEMKNYLKTVVAPQMPHEVYDSFIAAVDKGNIKSMP 328
Query: 407 NRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPLANLKDADALCN 466
NR+MPA+P PTPGALL+GDAFNMRHPLTGGGMTVALADIVVLR+LLRPL +L D +LC
Sbjct: 329 NRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALADIVVLRNLLRPLRDLSDGASLCK 388
Query: 467 YFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGLFSSGPVALL 526
Y ESFYTLRKPVA+TINTLA ALY+VFC+S + AR EMREACFDYL LGG+ +SGPV+LL
Sbjct: 389 YLESFYTLRKPVAATINTLANALYQVFCSSENEARNEMREACFDYLGLGGMCTSGPVSLL 448
Query: 527 SGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQ 586
SGLNPRPL+L CHFFAVA+YGV RLLIPFPSP RIW+G +LI+GA+GIIFPIIKAEGVRQ
Sbjct: 449 SGLNPRPLTLVCHFFAVAVYGVIRLLIPFPSPKRIWLGAKLISGASGIIFPIIKAEGVRQ 508
Query: 587 MFFPATVPAYYRAPP 596
MFFPATVPAYY P
Sbjct: 509 MFFPATVPAYYYKAP 523
BLAST of Moc04g08140 vs. ExPASy Swiss-Prot
Match:
Q8VYH2 (Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1)
HSP 1 Score: 748.4 bits (1931), Expect = 6.1e-215
Identity = 360/490 (73.47%), Postives = 423/490 (86.33%), Query Frame = 0
Query: 106 MIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDL 165
+++++ I GS N + D++IVGAGVAGAALA+TL K+GR+VHVIERDL
Sbjct: 28 VLRRRSKTIHGSVNVRNGTLTVKSGTDVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDL 87
Query: 166 AEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSH 225
EP+RIVGELLQPGGYLKLIELGLED V IDAQ+V GYAL+KDGKHTKL+YPL FDS
Sbjct: 88 TEPDRIVGELLQPGGYLKLIELGLEDCVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSD 147
Query: 226 VSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAP 285
V+GRSFHNGRF+QR+R+ A+ L NVR+EQGTVTS+VEE+G +KGVQYKTK+GQEL ++AP
Sbjct: 148 VAGRSFHNGRFVQRMREKASLLPNVRMEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAP 207
Query: 286 LTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENHGHVVLADPSPILLYPISS 345
LTIVCDG FSNLRR+LCKP++++PS FVGLVLENC+LPF NHGHVVL DPSPIL YPISS
Sbjct: 208 LTIVCDGCFSNLRRSLCKPKVEVPSNFVGLVLENCELPFPNHGHVVLGDPSPILFYPISS 267
Query: 346 TEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMP 405
+E+RCLVD+PG K+PSVA+GEMAH+LKT+VAPQVPP++ +FI+A+EKGNI++ NR+MP
Sbjct: 268 SEVRCLVDVPGSKLPSVASGEMAHHLKTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMP 327
Query: 406 AAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPLANLKDADALCNYFESF 465
A P TPGALLLGDAFNMRHPLTGGGMTVAL+DIV+LRDLL PL +L + ++L Y ESF
Sbjct: 328 ADPIHTPGALLLGDAFNMRHPLTGGGMTVALSDIVILRDLLNPLVDLTNKESLSKYIESF 387
Query: 466 YTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNP 525
YTLRKPVASTINTLAGALYKVF ASPD AR EMR ACFDYLSLGG+ SSGPVALLSGLNP
Sbjct: 388 YTLRKPVASTINTLAGALYKVFLASPDDARSEMRRACFDYLSLGGVCSSGPVALLSGLNP 447
Query: 526 RPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPA 585
RP+SL HFFAVAI+GV RLL+P PS R+W+G RLI+ A+GIIFPIIKAEGVRQMFFP
Sbjct: 448 RPMSLVLHFFAVAIFGVGRLLVPLPSVKRLWLGARLISSASGIIFPIIKAEGVRQMFFPR 507
Query: 586 TVPAYYRAPP 596
T+PA YRAPP
Sbjct: 508 TIPAIYRAPP 517
BLAST of Moc04g08140 vs. ExPASy Swiss-Prot
Match:
O81000 (Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1 SV=1)
HSP 1 Score: 745.3 bits (1923), Expect = 5.2e-214
Identity = 361/493 (73.23%), Postives = 416/493 (84.38%), Query Frame = 0
Query: 103 TDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIE 162
+ N KK + + V+ E + DV+IVGAGVAG+ALA+TL K+GR+VHVIE
Sbjct: 93 SSNKNKKNRGLVVSQNDTVSKNLETEVDSGTDVIIVGAGVAGSALAHTLGKEGRRVHVIE 152
Query: 163 RDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNF 222
RD +E +RIVGELLQPGGYLKLIELGLED V IDAQ+V GY L+KDGKHTKLAYPL+ F
Sbjct: 153 RDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKIDAQRVLGYVLFKDGKHTKLAYPLETF 212
Query: 223 DSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTA 282
DS V+GRSFHNGRF+QR+R+ A +LSNVRLEQGTVTS++EE GT+KGV+Y+TK G E +
Sbjct: 213 DSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGTVTSLLEEHGTIKGVRYRTKEGNEFRS 272
Query: 283 YAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENHGHVVLADPSPILLYP 342
+APLTIVCDG FSNLRR+LCKP++D+PS FVGLVLENC+LPF NHGHVVL DPSPIL+YP
Sbjct: 273 FAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLVLENCELPFANHGHVVLGDPSPILMYP 332
Query: 343 ISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASFIAAIEKGNIKSTTNR 402
ISS+E+RCLVD+PGQK+P +ANGEMA YLKT VAPQVP ++ +FI A+EKGNI++ NR
Sbjct: 333 ISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVAPQVPTKVREAFITAVEKGNIRTMPNR 392
Query: 403 TMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPLANLKDADALCNYF 462
+MPA P PTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRP+ NL D +AL Y
Sbjct: 393 SMPADPIPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPIRNLNDKEALSKYI 452
Query: 463 ESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGLFSSGPVALLSG 522
ESFYTLRKPVASTINTLA ALYKVF AS D AR EMREACFDYLSLGG+FSSGPVALLSG
Sbjct: 453 ESFYTLRKPVASTINTLADALYKVFLASSDEARTEMREACFDYLSLGGVFSSGPVALLSG 512
Query: 523 LNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMF 582
LNPRPLSL HFFAVAIY V RL++PFPS W+G R+I+ A+ IIFPIIKAEGVRQMF
Sbjct: 513 LNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFWLGARIISSASSIIFPIIKAEGVRQMF 572
Query: 583 FPATVPAYYRAPP 596
FP T+PA YRAPP
Sbjct: 573 FPRTIPAIYRAPP 585
BLAST of Moc04g08140 vs. ExPASy TrEMBL
Match:
A0A6J1E1H4 (Squalene monooxygenase OS=Momordica charantia OX=3673 GN=LOC111025080 PE=3 SV=1)
HSP 1 Score: 1019.6 bits (2635), Expect = 5.2e-294
Identity = 507/510 (99.41%), Postives = 507/510 (99.41%), Query Frame = 0
Query: 88 FRARRFNNGISNKTNTDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAAL 147
F RFNNGISNKTNTDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAAL
Sbjct: 23 FAHLRFNNGISNKTNTDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAAL 82
Query: 148 AYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALY 207
AYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALY
Sbjct: 83 AYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALY 142
Query: 208 KDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTV 267
KDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTV
Sbjct: 143 KDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTV 202
Query: 268 KGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENH 327
KGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENH
Sbjct: 203 KGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENH 262
Query: 328 GHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASF 387
GHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASF
Sbjct: 263 GHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASF 322
Query: 388 IAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLR 447
IAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLR
Sbjct: 323 IAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLR 382
Query: 448 PLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLS 507
PLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLS
Sbjct: 383 PLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLS 442
Query: 508 LGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAG 567
LGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAG
Sbjct: 443 LGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAG 502
Query: 568 IIFPIIKAEGVRQMFFPATVPAYYRAPPMN 598
IIFPIIKAEGVRQMFFPATVPAYYRAPPMN
Sbjct: 503 IIFPIIKAEGVRQMFFPATVPAYYRAPPMN 532
BLAST of Moc04g08140 vs. ExPASy TrEMBL
Match:
A0A6J1GKV9 (Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111454931 PE=3 SV=1)
HSP 1 Score: 879.0 bits (2270), Expect = 1.1e-251
Identity = 427/467 (91.43%), Postives = 453/467 (97.00%), Query Frame = 0
Query: 130 SNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGL 189
S++ DV++VGAGVAGAALAYTLAKDGRKVHVIERDL+EPERIVGELLQPGGYLKLIELGL
Sbjct: 58 SDDLDVIVVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGL 117
Query: 190 EDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSN 249
EDS+ GIDAQQVFGYAL+KDG HT+LAYPL FDS++SGRSFHNGRFIQRLRQ AA+LSN
Sbjct: 118 EDSLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSN 177
Query: 250 VRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIP 309
V+LEQGTVTSIVEEDGTVKGVQYKTKNGQELT+YAPLTIVCDGGFSNLRRNLCKPQIDIP
Sbjct: 178 VKLEQGTVTSIVEEDGTVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIP 237
Query: 310 SCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAH 369
SCFVGLVLENCKLP ENHGHVVLADPSPILLY ISSTEIRCLVDIPGQKVPSVANG+MAH
Sbjct: 238 SCFVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAH 297
Query: 370 YLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTG 429
YLKTVVAPQ+PPEL +FIA+++KGNIKSTTNR+MPAAP PTPGALLLGDAFNMRHPLTG
Sbjct: 298 YLKTVVAPQLPPELQTAFIASVDKGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTG 357
Query: 430 GGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCA 489
GGMTVALADIVVLRDLLRPL+NL DADALCNY ESFYTLRKPVASTINTLAGALY+VFCA
Sbjct: 358 GGMTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCA 417
Query: 490 SPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPF 549
SPDPARKEMREACFDYLSLGG+FSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV+RLLIPF
Sbjct: 418 SPDPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPF 477
Query: 550 PSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRAPPM 597
PSP RIWIGVRLITGAAGIIFPIIKAEGVRQMFFPAT+PAYYRAPPM
Sbjct: 478 PSPKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 524
BLAST of Moc04g08140 vs. ExPASy TrEMBL
Match:
A0A5A7SMZ2 (Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001510 PE=3 SV=1)
HSP 1 Score: 878.2 bits (2268), Expect = 1.9e-251
Identity = 433/504 (85.91%), Postives = 465/504 (92.26%), Query Frame = 0
Query: 93 FNNGISNKTNTDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAALAYTLA 152
F +GI+N ++ N I K I + A PP + + PDV++VGAGVAGAALA+TLA
Sbjct: 25 FKDGINN-SSPPNPIHSKSINSPSAAALQIPPHSD--DDHPDVIVVGAGVAGAALAFTLA 84
Query: 153 KDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALYKDGKH 212
KDGRKVHVIERDL EP+RIVGELLQPGGYLKLIELGLEDS+NGIDAQQVFGYALYK+G H
Sbjct: 85 KDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDSLNGIDAQQVFGYALYKNGSH 144
Query: 213 TKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTVKGVQY 272
T+LAYPL+ FDS+VSGRSFHNGRFIQRLR+ AA+LSNVR+EQGTVTSIVEEDG VKGVQY
Sbjct: 145 TRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVRMEQGTVTSIVEEDGIVKGVQY 204
Query: 273 KTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENHGHVVL 332
KTKNG+ELTAYAPLTIVCDGGFSNLRR LCKPQIDIPSCFVGLVLENCKLP ENHGHVVL
Sbjct: 205 KTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSCFVGLVLENCKLPLENHGHVVL 264
Query: 333 ADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASFIAAIE 392
ADPSPILLYPISSTE+RCLVDIPGQKVPSVANGEMAHYLKT VAPQ+PPE++ +FIAAI+
Sbjct: 265 ADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYLKTAVAPQLPPEVYPAFIAAID 324
Query: 393 KGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPLANL 452
KGNIKSTTNR+MPAAP+ TPGALLLGDAFNMRHPLTGGGMTVAL+DIVVLRDLLRPL+NL
Sbjct: 325 KGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGGMTVALSDIVVLRDLLRPLSNL 384
Query: 453 KDADALCNYFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGLF 512
DAD LCNY ESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGG+F
Sbjct: 385 NDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGVF 444
Query: 513 SSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAGIIFPI 572
SSGPVALLSGLNPRPLSLFCHFFAVAIYGV RLLIPFPSP RIWIGVRLITGAAGIIFPI
Sbjct: 445 SSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPSPKRIWIGVRLITGAAGIIFPI 504
Query: 573 IKAEGVRQMFFPATVPAYYRAPPM 597
IK EGVRQMFFPATVPAYYR PP+
Sbjct: 505 IKDEGVRQMFFPATVPAYYRTPPI 525
BLAST of Moc04g08140 vs. ExPASy TrEMBL
Match:
A0A345Z0U9 (Squalene monooxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)
HSP 1 Score: 877.5 bits (2266), Expect = 3.2e-251
Identity = 427/467 (91.43%), Postives = 452/467 (96.79%), Query Frame = 0
Query: 130 SNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGL 189
S++ DV+IVGAGVAGAALAYTLAKDGRKVHVIERDL+EPERIVGELLQPGGYLKLIELGL
Sbjct: 57 SDDFDVIIVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGL 116
Query: 190 EDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSN 249
EDS+ GIDAQQVFGYAL+KDG HT+LAYPL FDS++SGRSFHNGRFIQRLRQ AA+LSN
Sbjct: 117 EDSLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSN 176
Query: 250 VRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIP 309
V+LEQGTVTSIVEEDGTVKGVQYKTKNGQELT+YAPLTIVCDGGFSNLRRNLCKPQIDIP
Sbjct: 177 VKLEQGTVTSIVEEDGTVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIP 236
Query: 310 SCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAH 369
SCFVGLVLENCKLP ENHGHVVLADPSPILLY ISSTEIRCLVDIPGQKVPSVANG+MAH
Sbjct: 237 SCFVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAH 296
Query: 370 YLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTG 429
YLKT VAPQ+PPEL +FIA+++KGNIKSTTNR+MPAAP PTPGALLLGDAFNMRHPLTG
Sbjct: 297 YLKTAVAPQLPPELQTAFIASVDKGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTG 356
Query: 430 GGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCA 489
GGMTVALADIVVLRDLLRPL+NL DADALCNY ESFYTLRKPVASTINTLAGALY+VFCA
Sbjct: 357 GGMTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCA 416
Query: 490 SPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPF 549
SPDPARKEMREACFDYLSLGG+FSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV+RLLIPF
Sbjct: 417 SPDPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPF 476
Query: 550 PSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRAPPM 597
PSP RIWIGVRLITGAAGIIFPIIKAEGVRQMFFPAT+PAYYRAPPM
Sbjct: 477 PSPKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 523
BLAST of Moc04g08140 vs. ExPASy TrEMBL
Match:
A0A6J1I4X3 (Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111471032 PE=3 SV=1)
HSP 1 Score: 872.8 bits (2254), Expect = 7.9e-250
Identity = 425/467 (91.01%), Postives = 450/467 (96.36%), Query Frame = 0
Query: 130 SNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLIELGL 189
S++ DV++VGAGVAGAALAYTLAKDGRKVHVIERDL+EPERIVGELLQPGGYLKLIELGL
Sbjct: 57 SDDFDVIVVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGL 116
Query: 190 EDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSHVSGRSFHNGRFIQRLRQMAASLSN 249
EDS+ GIDAQQVFGYAL+KDG HT+LAYPL FDS++SGRSFHNGRFIQRLRQ AA+LSN
Sbjct: 117 EDSLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSN 176
Query: 250 VRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCKPQIDIP 309
V+LEQGTVTSIVEEDG VKGVQYKTKNGQELT+YAPLTIVCDGGFSNLRRNLCKPQIDIP
Sbjct: 177 VKLEQGTVTSIVEEDGNVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIP 236
Query: 310 SCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVANGEMAH 369
SCFVGLVLENCKLP ENHGHVVLADPSPILLY ISSTEIRCLVDIPGQKVPSVANG+MA
Sbjct: 237 SCFVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAR 296
Query: 370 YLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMPAAPRPTPGALLLGDAFNMRHPLTG 429
YLKTVVAPQ+PPEL +FIA++E GNIKSTTNR+MPAAP PTPGALLLGDAFNMRHPLTG
Sbjct: 297 YLKTVVAPQLPPELQTAFIASVENGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTG 356
Query: 430 GGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLAGALYKVFCA 489
GGMTVALADIVVLRDLLRPL+NL DADALCNY ESFYTLRKPVASTINTLAGALY+VFCA
Sbjct: 357 GGMTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCA 416
Query: 490 SPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVSRLLIPF 549
SPDPARKEMREACFDYLSLGG+FSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV+RLLIPF
Sbjct: 417 SPDPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPF 476
Query: 550 PSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRAPPM 597
PSP RIWIGVRLITGAAGIIFPIIKAEGVRQMFFPAT+PAYYRAPPM
Sbjct: 477 PSPKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 523
BLAST of Moc04g08140 vs. TAIR 10
Match:
AT1G58440.1 (FAD/NAD(P)-binding oxidoreductase family protein )
HSP 1 Score: 752.3 bits (1941), Expect = 3.0e-217
Identity = 363/495 (73.33%), Postives = 427/495 (86.26%), Query Frame = 0
Query: 107 IKKKKIFIAGSRAAVNPPSENVQS------NEPDVVIVGAGVAGAALAYTLAKDGRKVHV 166
+K K+ + R V+ + +V S DV++VGAGVAG+ALAYTL KD R+VHV
Sbjct: 29 VKPKRNGLRHDRKTVSTVTSDVGSVNITGDTVADVIVVGAGVAGSALAYTLGKDKRRVHV 88
Query: 167 IERDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPLQ 226
IERDL+EP+RIVGELLQPGGYLKL+ELG+ED V IDAQ+V+GYAL+K+GK +LAYPL+
Sbjct: 89 IERDLSEPDRIVGELLQPGGYLKLLELGIEDCVEEIDAQRVYGYALFKNGKRIRLAYPLE 148
Query: 227 NFDSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQEL 286
F VSGRSFHNGRFIQR+R+ AASL NV+LEQGTV S++EE+GT+KGV+YK K G+E
Sbjct: 149 KFHEDVSGRSFHNGRFIQRMREKAASLPNVQLEQGTVLSLLEENGTIKGVRYKNKAGEEQ 208
Query: 287 TAYAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENHGHVVLADPSPILL 346
TA+A LTIVCDG FSNLRR+LC PQ+++PSCFVGLVLENC LP+ NHGHVVLADPSPIL+
Sbjct: 209 TAFAALTIVCDGCFSNLRRSLCNPQVEVPSCFVGLVLENCNLPYANHGHVVLADPSPILM 268
Query: 347 YPISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASFIAAIEKGNIKSTT 406
YPISSTE+RCLVD+PGQKVPS+ANGEM +YLKTVVAPQ+P E++ SFIAA++KGNIKS
Sbjct: 269 YPISSTEVRCLVDVPGQKVPSIANGEMKNYLKTVVAPQMPHEVYDSFIAAVDKGNIKSMP 328
Query: 407 NRTMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPLANLKDADALCN 466
NR+MPA+P PTPGALL+GDAFNMRHPLTGGGMTVALADIVVLR+LLRPL +L D +LC
Sbjct: 329 NRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALADIVVLRNLLRPLRDLSDGASLCK 388
Query: 467 YFESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGLFSSGPVALL 526
Y ESFYTLRKPVA+TINTLA ALY+VFC+S + AR EMREACFDYL LGG+ +SGPV+LL
Sbjct: 389 YLESFYTLRKPVAATINTLANALYQVFCSSENEARNEMREACFDYLGLGGMCTSGPVSLL 448
Query: 527 SGLNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQ 586
SGLNPRPL+L CHFFAVA+YGV RLLIPFPSP RIW+G +LI+GA+GIIFPIIKAEGVRQ
Sbjct: 449 SGLNPRPLTLVCHFFAVAVYGVIRLLIPFPSPKRIWLGAKLISGASGIIFPIIKAEGVRQ 508
Query: 587 MFFPATVPAYYRAPP 596
MFFPATVPAYY P
Sbjct: 509 MFFPATVPAYYYKAP 523
BLAST of Moc04g08140 vs. TAIR 10
Match:
AT4G37760.1 (squalene epoxidase 3 )
HSP 1 Score: 748.4 bits (1931), Expect = 4.3e-216
Identity = 360/490 (73.47%), Postives = 423/490 (86.33%), Query Frame = 0
Query: 106 MIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDL 165
+++++ I GS N + D++IVGAGVAGAALA+TL K+GR+VHVIERDL
Sbjct: 28 VLRRRSKTIHGSVNVRNGTLTVKSGTDVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDL 87
Query: 166 AEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNFDSH 225
EP+RIVGELLQPGGYLKLIELGLED V IDAQ+V GYAL+KDGKHTKL+YPL FDS
Sbjct: 88 TEPDRIVGELLQPGGYLKLIELGLEDCVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSD 147
Query: 226 VSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAP 285
V+GRSFHNGRF+QR+R+ A+ L NVR+EQGTVTS+VEE+G +KGVQYKTK+GQEL ++AP
Sbjct: 148 VAGRSFHNGRFVQRMREKASLLPNVRMEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAP 207
Query: 286 LTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENHGHVVLADPSPILLYPISS 345
LTIVCDG FSNLRR+LCKP++++PS FVGLVLENC+LPF NHGHVVL DPSPIL YPISS
Sbjct: 208 LTIVCDGCFSNLRRSLCKPKVEVPSNFVGLVLENCELPFPNHGHVVLGDPSPILFYPISS 267
Query: 346 TEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASFIAAIEKGNIKSTTNRTMP 405
+E+RCLVD+PG K+PSVA+GEMAH+LKT+VAPQVPP++ +FI+A+EKGNI++ NR+MP
Sbjct: 268 SEVRCLVDVPGSKLPSVASGEMAHHLKTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMP 327
Query: 406 AAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPLANLKDADALCNYFESF 465
A P TPGALLLGDAFNMRHPLTGGGMTVAL+DIV+LRDLL PL +L + ++L Y ESF
Sbjct: 328 ADPIHTPGALLLGDAFNMRHPLTGGGMTVALSDIVILRDLLNPLVDLTNKESLSKYIESF 387
Query: 466 YTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNP 525
YTLRKPVASTINTLAGALYKVF ASPD AR EMR ACFDYLSLGG+ SSGPVALLSGLNP
Sbjct: 388 YTLRKPVASTINTLAGALYKVFLASPDDARSEMRRACFDYLSLGGVCSSGPVALLSGLNP 447
Query: 526 RPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPA 585
RP+SL HFFAVAI+GV RLL+P PS R+W+G RLI+ A+GIIFPIIKAEGVRQMFFP
Sbjct: 448 RPMSLVLHFFAVAIFGVGRLLVPLPSVKRLWLGARLISSASGIIFPIIKAEGVRQMFFPR 507
Query: 586 TVPAYYRAPP 596
T+PA YRAPP
Sbjct: 508 TIPAIYRAPP 517
BLAST of Moc04g08140 vs. TAIR 10
Match:
AT2G22830.1 (squalene epoxidase 2 )
HSP 1 Score: 745.3 bits (1923), Expect = 3.7e-215
Identity = 361/493 (73.23%), Postives = 416/493 (84.38%), Query Frame = 0
Query: 103 TDNMIKKKKIFIAGSRAAVNPPSENVQSNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIE 162
+ N KK + + V+ E + DV+IVGAGVAG+ALA+TL K+GR+VHVIE
Sbjct: 93 SSNKNKKNRGLVVSQNDTVSKNLETEVDSGTDVIIVGAGVAGSALAHTLGKEGRRVHVIE 152
Query: 163 RDLAEPERIVGELLQPGGYLKLIELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPLQNF 222
RD +E +RIVGELLQPGGYLKLIELGLED V IDAQ+V GY L+KDGKHTKLAYPL+ F
Sbjct: 153 RDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKIDAQRVLGYVLFKDGKHTKLAYPLETF 212
Query: 223 DSHVSGRSFHNGRFIQRLRQMAASLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTA 282
DS V+GRSFHNGRF+QR+R+ A +LSNVRLEQGTVTS++EE GT+KGV+Y+TK G E +
Sbjct: 213 DSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGTVTSLLEEHGTIKGVRYRTKEGNEFRS 272
Query: 283 YAPLTIVCDGGFSNLRRNLCKPQIDIPSCFVGLVLENCKLPFENHGHVVLADPSPILLYP 342
+APLTIVCDG FSNLRR+LCKP++D+PS FVGLVLENC+LPF NHGHVVL DPSPIL+YP
Sbjct: 273 FAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLVLENCELPFANHGHVVLGDPSPILMYP 332
Query: 343 ISSTEIRCLVDIPGQKVPSVANGEMAHYLKTVVAPQVPPELHASFIAAIEKGNIKSTTNR 402
ISS+E+RCLVD+PGQK+P +ANGEMA YLKT VAPQVP ++ +FI A+EKGNI++ NR
Sbjct: 333 ISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVAPQVPTKVREAFITAVEKGNIRTMPNR 392
Query: 403 TMPAAPRPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPLANLKDADALCNYF 462
+MPA P PTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRP+ NL D +AL Y
Sbjct: 393 SMPADPIPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPIRNLNDKEALSKYI 452
Query: 463 ESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGLFSSGPVALLSG 522
ESFYTLRKPVASTINTLA ALYKVF AS D AR EMREACFDYLSLGG+FSSGPVALLSG
Sbjct: 453 ESFYTLRKPVASTINTLADALYKVFLASSDEARTEMREACFDYLSLGGVFSSGPVALLSG 512
Query: 523 LNPRPLSLFCHFFAVAIYGVSRLLIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMF 582
LNPRPLSL HFFAVAIY V RL++PFPS W+G R+I+ A+ IIFPIIKAEGVRQMF
Sbjct: 513 LNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFWLGARIISSASSIIFPIIKAEGVRQMF 572
Query: 583 FPATVPAYYRAPP 596
FP T+PA YRAPP
Sbjct: 573 FPRTIPAIYRAPP 585
BLAST of Moc04g08140 vs. TAIR 10
Match:
AT5G24150.1 (FAD/NAD(P)-binding oxidoreductase family protein )
HSP 1 Score: 483.0 bits (1242), Expect = 3.4e-136
Identity = 240/471 (50.96%), Postives = 323/471 (68.58%), Query Frame = 0
Query: 126 ENVQSNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKLI 185
E + DV+IVGAGV G+ALAY LAKDGR+VHVIERDL EPERI+GE +QPGG L L
Sbjct: 39 EEREDGATDVIIVGAGVGGSALAYALAKDGRRVHVIERDLREPERIMGEFMQPGGRLMLS 98
Query: 186 ELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPL--QNFDSHVSGRSFHNGRFIQRLRQM 245
+LGLED + GIDAQ+ G +YKDGK ++P+ NF S RSFHNGRF+QRLRQ
Sbjct: 99 KLGLEDCLEGIDAQKATGMTVYKDGKEAVASFPVDNNNFPFDPSARSFHNGRFVQRLRQK 158
Query: 246 AASLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLCK 305
A+SL NVRLE+GTV S++EE G +KGV YK G+E TA APLT+VCDG +SNLRR+L
Sbjct: 159 ASSLPNVRLEEGTVKSLIEEKGVIKGVTYKNSAGEETTALAPLTVVCDGCYSNLRRSLND 218
Query: 306 PQIDIPSCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPSVA 365
++ S VG + +NC+L ++++ PS +LY ISST++RC+ ++ +PS++
Sbjct: 219 NNAEVLSYQVGFISKNCQLEEPEKLKLIMSKPSFTMLYQISSTDVRCVFEVLPNNIPSIS 278
Query: 366 NGEMAHYLKTVVAPQVPPELHASFIAAIEKG-NIKSTTNRTMPAAPRPTPGALLLGDAFN 425
NGEMA ++K +APQVP +L F+ I++G +IK+ + M A G +LLGDAFN
Sbjct: 279 NGEMATFVKNTIAPQVPLKLRKIFLKGIDEGEHIKAMPTKKMTATLSEKKGVILLGDAFN 338
Query: 426 MRHPLTGGGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLAGA 485
MRHP GM V L+DI++LR LL+PL+NL +A + +SFY +RKP+++T+NTL A
Sbjct: 339 MRHPAIASGMMVLLSDILILRRLLQPLSNLGNAQKISQVIKSFYDIRKPMSATVNTLGNA 398
Query: 486 LYKVFCASPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIYGV 545
+V AS D A++ MR+ C+DYLS GG +SG +ALL G+NPRP+SL H A+ + +
Sbjct: 399 FSQVLVASTDEAKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSI 458
Query: 546 SRLLIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRA 594
LL PFPSP RIW +RL A ++ P +KAEGV QM FP AY ++
Sbjct: 459 GHLLSPFPSPLRIWHSLRLFGLAMKMLVPHLKAEGVSQMLFPVNAAAYSKS 509
BLAST of Moc04g08140 vs. TAIR 10
Match:
AT5G24160.1 (squalene monoxygenase 6 )
HSP 1 Score: 474.9 bits (1221), Expect = 9.3e-134
Identity = 237/473 (50.11%), Postives = 325/473 (68.71%), Query Frame = 0
Query: 125 SENVQSNEPDVVIVGAGVAGAALAYTLAKDGRKVHVIERDLAEPERIVGELLQPGGYLKL 184
+E+ + DV+IVGAGV G+ALAY LAKDGR+VHVIERD+ EPER++GE +QPGG L L
Sbjct: 38 AEDQKDGAADVIIVGAGVGGSALAYALAKDGRRVHVIERDMREPERMMGEFMQPGGRLML 97
Query: 185 IELGLEDSVNGIDAQQVFGYALYKDGKHTKLAYPL--QNFDSHVSGRSFHNGRFIQRLRQ 244
+LGL+D + IDAQ+ G A+YKDGK +P+ NF S RSFHNGRF+Q+LR+
Sbjct: 98 SKLGLQDCLEDIDAQKATGLAVYKDGKEADAPFPVDNNNFSYEPSARSFHNGRFVQQLRR 157
Query: 245 MAASLSNVRLEQGTVTSIVEEDGTVKGVQYKTKNGQELTAYAPLTIVCDGGFSNLRRNLC 304
A SLSNVRLE+GTV S++EE G VKGV YK K G+E TA APLT+VCDG +SNLRR+L
Sbjct: 158 KAFSLSNVRLEEGTVKSLLEEKGVVKGVTYKNKEGEETTALAPLTVVCDGCYSNLRRSLN 217
Query: 305 KP-QIDIPSCFVGLVLENCKLPFENHGHVVLADPSPILLYPISSTEIRCLVDIPGQKVPS 364
+I S VG + +NC+L H++L+ PS ++Y ISST++RC ++ + PS
Sbjct: 218 DDNNAEIMSYIVGYISKNCRLEEPEKLHLILSKPSFTMVYQISSTDVRCGFEVLPENFPS 277
Query: 365 VANGEMAHYLKTVVAPQVPPELHASFIAAIEKG-NIKSTTNRTMPAAPRPTPGALLLGDA 424
+ANGEM+ ++K + PQVPP+L F+ I++G +IK + M + G ++LGDA
Sbjct: 278 IANGEMSTFMKNTIVPQVPPKLRKIFLKGIDEGAHIKVVPAKRMTSTLSKKKGVIVLGDA 337
Query: 425 FNMRHPLTGGGMTVALADIVVLRDLLRPLANLKDADALCNYFESFYTLRKPVASTINTLA 484
FNMRHP+ GM V L+DI++LR LL+PL+NL DA+ + SFY +RKP+++T+NTL
Sbjct: 338 FNMRHPVVASGMMVLLSDILILRRLLQPLSNLGDANKVSEVINSFYDIRKPMSATVNTLG 397
Query: 485 GALYKVFCASPDPARKEMREACFDYLSLGGLFSSGPVALLSGLNPRPLSLFCHFFAVAIY 544
A +V S D A++ MR+ +DYL GG +SG +ALL G+NPRPLSL H A+ +
Sbjct: 398 NAFSQVLIGSTDEAKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLS 457
Query: 545 GVSRLLIPFPSPTRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRA 594
+ +LL PFPSP RIW ++L A ++ P +KAEGV QM FPA AY+++
Sbjct: 458 SIGQLLSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022158631.1 | 1.1e-293 | 99.41 | squalene monooxygenase-like [Momordica charantia] | [more] |
XP_038887697.1 | 5.4e-253 | 86.51 | squalene monooxygenase SE2-like [Benincasa hispida] | [more] |
XP_022952179.1 | 2.3e-251 | 91.43 | squalene epoxidase 1-like isoform X1 [Cucurbita moschata] | [more] |
KAG6571900.1 | 3.0e-251 | 91.65 | Squalene monooxygenase SE2, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
KAG7011588.1 | 3.0e-251 | 91.65 | Squalene epoxidase 1 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
Match Name | E-value | Identity | Description | |
B7TWW5 | 7.2e-224 | 78.81 | Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2 | [more] |
O48651 | 2.0e-221 | 77.97 | Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1 | [more] |
Q9SM02 | 4.2e-216 | 73.33 | Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1 | [more] |
Q8VYH2 | 6.1e-215 | 73.47 | Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1 | [more] |
O81000 | 5.2e-214 | 73.23 | Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1E1H4 | 5.2e-294 | 99.41 | Squalene monooxygenase OS=Momordica charantia OX=3673 GN=LOC111025080 PE=3 SV=1 | [more] |
A0A6J1GKV9 | 1.1e-251 | 91.43 | Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111454931 PE=3 SV=1 | [more] |
A0A5A7SMZ2 | 1.9e-251 | 85.91 | Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... | [more] |
A0A345Z0U9 | 3.2e-251 | 91.43 | Squalene monooxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1 | [more] |
A0A6J1I4X3 | 7.9e-250 | 91.01 | Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111471032 PE=3 SV=1 | [more] |