Moc04g07150 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g07150
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H protein
Location	chr4: 5054656 .. 5055474 (+)
RNA-Seq Expression	Moc04g07150
Synteny	Moc04g07150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGTCTTCCCTGTTTGTCCTATTTGCCTTGAAGAGGATGAGACAACGGACCACACCTTAGTGGGTTGCAATAAGGCGGGCCAGATTTGGGACATTTCCCTTCCTATGGTGATGGGCCTTGGTGTCGATTGCAACTGCAGTTTCATTGATAGATGGGCGCTTTGGAGAGAAAACCTAACACCAAAGGAATTGCCTTTAGCGGGCATCATTTGTTGGGCGAATTGGAATGACAAAAACCAAAGAAAAAATGGTGAAGTCGTTGCGGATGTTCACACAAAATCTGATTGGATCACAAATTATGCTATGGAGCTTTTAACTCGGAGAAGAAAGACTAACGAGGCCCAGAAGAATTTACCTTGTCCGGCGATTTGAAAACATCCTTCGAAAGGAACAGTCAAGCTCAATGTGGACGTGCCTTTCGATGTCCGAAATCAAAGGGGAGGGGTTGGGGTTTTGGTTTGTGATGATCAGAACTCCATCCTAGCAGCCTTAATTTCATCCCACAATGTGAATTCCCCTCTGTTGGTCGAAATTTGTGCTATTCGTGAAGTAGTTTGCCTAGCTGAGAGGTTAGGTCTTCAGTTGGTTGTTTTTTAGTCGGATTCCCTTGAAGCAGTTGAGCTTATCAATGGGATTTCGGTCAAAACCAACGAATTCGGAACCTGGATTGAGGATATTCGAATTTTGGCTGACAATTTTGTGCTTTTCAGATGTATACATGTCGGAAGAGAGGCTAACAAACCGACTCATTTCCTTATGGGTGAGGCTCTGCATCAGAATAATCCAGTATTGTGGCTTTCTGATTTCCCTCCTTGA

mRNA sequence

ATGGATGTCTTCCCTGTTTGTCCTATTTGCCTTGAAGAGGATGAGACAACGGACCACACCTTAGTGGGTTGCAATAAGGCGGGCCAGATTTGGGACATTTCCCTTCCTATGGTGATGGGCCTTGGTGTCGATTGCAACTGCAGTTTCATTGATAGATGGGCGCTTTGGAGAGAAAACCTAACACCAAAGGAATTGCCTTTAGCGGGCATCATTTGTTGGGCGAATTGGAATGACAAAAACCAAAGAAAAAATGGTGAAGTCGTTGCGGATGTTCACACAAAATCTGATTGGATCACAAATTATGCTATGGAGCTTTTAACTCGGAGAAGAAAGACTAACGAGGCCCAGAAGAATTTACCTTCCTTAATTTCATCCCACAATGTGAATTCCCCTCTGTTGGTCGAAATTTGTGCTATTCGTGAAGTAGTTTGCCTAGCTGAGAGATGTATACATGTCGGAAGAGAGGCTAACAAACCGACTCATTTCCTTATGGGTGAGGCTCTGCATCAGAATAATCCAGTATTGTGGCTTTCTGATTTCCCTCCTTGA

Coding sequence (CDS)

ATGGATGTCTTCCCTGTTTGTCCTATTTGCCTTGAAGAGGATGAGACAACGGACCACACCTTAGTGGGTTGCAATAAGGCGGGCCAGATTTGGGACATTTCCCTTCCTATGGTGATGGGCCTTGGTGTCGATTGCAACTGCAGTTTCATTGATAGATGGGCGCTTTGGAGAGAAAACCTAACACCAAAGGAATTGCCTTTAGCGGGCATCATTTGTTGGGCGAATTGGAATGACAAAAACCAAAGAAAAAATGGTGAAGTCGTTGCGGATGTTCACACAAAATCTGATTGGATCACAAATTATGCTATGGAGCTTTTAACTCGGAGAAGAAAGACTAACGAGGCCCAGAAGAATTTACCTTCCTTAATTTCATCCCACAATGTGAATTCCCCTCTGTTGGTCGAAATTTGTGCTATTCGTGAAGTAGTTTGCCTAGCTGAGAGATGTATACATGTCGGAAGAGAGGCTAACAAACCGACTCATTTCCTTATGGGTGAGGCTCTGCATCAGAATAATCCAGTATTGTGGCTTTCTGATTTCCCTCCTTGA

Protein sequence

MDVFPVCPICLEEDETTDHTLVGCNKAGQIWDISLPMVMGLGVDCNCSFIDRWALWRENLTPKELPLAGIICWANWNDKNQRKNGEVVADVHTKSDWITNYAMELLTRRRKTNEAQKNLPSLISSHNVNSPLLVEICAIREVVCLAERCIHVGREANKPTHFLMGEALHQNNPVLWLSDFPP

Homology

[Close]

BLAST of Moc04g07150 vs. NCBI nr
Match: XP_022141766.1 (uncharacterized protein LOC111012047 [Momordica charantia])

HSP 1 Score: 247.7 bits (631), Expect = 7.9e-62
Identity = 125/186 (67.20%), Postives = 131/186 (70.43%), Query Frame = 0


Query: 38  VMGLGVDCNCSFIDRWALWRENLTPKELPLAGIICWANWNDKNQRKNGEVVADVHTKSDW 97
           +MGLGVDCNCSFIDRWALWRENLT +EL LAGIICWANWND+NQR NG  VADVHT+SDW
Sbjct: 1   MMGLGVDCNCSFIDRWALWRENLTLEELLLAGIICWANWNDRNQRNNGIDVADVHTESDW 60

Query: 98  ITNYAMELLTRRRKTNEAQKNLP------------------------------------- 157
           ITNYAMELLTRRRKTNEAQKNLP                                     
Sbjct: 61  ITNYAMELLTRRRKTNEAQKNLPCLAIWKPPSKGTVKLNVDAAFDVRNQRGGVGVLVRDD 120

Query: 158 ------SLISSHNVNSPLLVEICAIREVVCLAERCIHVGREANKPTHFLMGEALHQNNPV 181
                 +LISSHNVNSPLL EICAIRE V LAERCIHVGRE NKP HFL GEALHQN+ V
Sbjct: 121 RNSILAALISSHNVNSPLLAEICAIREAVRLAERCIHVGREGNKPAHFLAGEALHQNHLV 180

[Close]

BLAST of Moc04g07150 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 84.0 bits (206), Expect = 1.5e-12
Identity = 36/97 (37.11%), Postives = 52/97 (53.61%), Query Frame = 0


Query: 5    PVCPICLEEDETTDHTLVGCNKAGQIWDISLPMVMGLGVDCNCSFIDRWALWRENLTPKE 64
            P C IC +  E+  H    C +A QIW    P +  L  + N SF++ W+   E L PK+
Sbjct: 1171 PACTICGDRRESIIHAFFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKD 1230

Query: 65   LPLAGIICWANWNDKNQRKNGEVVADVHTKSDWITNY 102
            L LA I  W  WND+N   +G+ V+ V  K +W+T +
Sbjct: 1231 LNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPF 1267

[Close]

BLAST of Moc04g07150 vs. NCBI nr
Match: XP_022145060.1 (uncharacterized protein LOC111014578 [Momordica charantia])

HSP 1 Score: 76.6 bits (187), Expect = 2.4e-10
Identity = 37/108 (34.26%), Postives = 54/108 (50.00%), Query Frame = 0


Query: 1   MDVFPVCPICLEEDETTDHTLVGCNKAGQIWDISLPMVMGLGVDCNCSFIDRWALWRENL 60
           +D  P C +C ++ ETTDH L  C +A ++W+I LP       D N S  D      E+L
Sbjct: 22  IDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAFP-QCDFNNSVQDGLLYLVESL 81

Query: 61  TPKELPLAGIICWANWNDKNQRKNGEVVADVHTKSDWITNYAMELLTR 109
           +  +  L G+  WA WND+N  +    + D   +SDWI  Y  +   R
Sbjct: 82  STPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILTYVRDFQVR 128

[Close]

BLAST of Moc04g07150 vs. NCBI nr
Match: KAA0067681.1 (UMP-CMP kinase isoform X1 [Cucumis melo var. makuwa] >TYK23685.1 UMP-CMP kinase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 74.7 bits (182), Expect = 9.1e-10
Identity = 50/171 (29.24%), Postives = 79/171 (46.20%), Query Frame = 0


Query: 1   MDVFPVCPICLEEDETTDHTLVGCNKAGQIWDISLPMVMGLGVDCNCSFIDRWALWRENL 60
           MD+ P+CP C +  ET    + GC  A ++W   LP+V+  G +    F D   +  ++L
Sbjct: 140 MDISPLCPYCGKHPETITQVVFGCRLAKELWKCFLPVVLS-GRNYYFDFSDFCVVLADSL 199

Query: 61  TPKELPLAGIICWANWNDKNQRKNGEVVADVHTKSDWITNYAMELLTRRRKTNEAQKNL- 120
           +P +  L  I CW  W+D N  +   VV DV  K  W+++Y    +     T+    N+ 
Sbjct: 200 SPLDFSLWCIGCWGLWDDHNDFRVCGVVLDVDAKCWWLSSY----VRGNGSTSSPGWNIW 259

Query: 121 ---------------PSLISSHNVNSPLLVEICAIREVVCLAERCIHVGRE 156
                           +     ++NSPLL+E  AI+E + LA    H+G E
Sbjct: 260 GVGVVNVDANGDVLGATAKQFSSLNSPLLLETLAIKEGISLAS-APHLGDE 304

[Close]

BLAST of Moc04g07150 vs. NCBI nr
Match: KAF7823736.1 (putative ribonuclease H-like domain-containing protein [Senna tora])

HSP 1 Score: 61.2 bits (147), Expect = 1.0e-05
Identity = 33/86 (38.37%), Postives = 44/86 (51.16%), Query Frame = 0


Query: 1  MDVFPVCPICLEEDETTDHTLVGCNKAGQIW-DISLPMVMGLGVDCNCSFIDRW-----A 60
          MDV P+CPIC  EDE+  HT +GC++ G IW   ++P V     DC   F++ W     A
Sbjct: 1  MDVLPICPICYFEDESVIHTPLGCSQLGDIWKKTNIPFVEEF--DCEIPFVE-WLESAIA 60

Query: 61 LWRENLTPKELPLAGIICWANWNDKN 81
           W  N          IIC+  WN +N
Sbjct: 61 QWNAN----HFASFAIICYLLWNRRN 79

[Close]

BLAST of Moc04g07150 vs. ExPASy TrEMBL
Match: A0A6J1CK80 (uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012047 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 3.8e-62
Identity = 125/186 (67.20%), Postives = 131/186 (70.43%), Query Frame = 0


Query: 38  VMGLGVDCNCSFIDRWALWRENLTPKELPLAGIICWANWNDKNQRKNGEVVADVHTKSDW 97
           +MGLGVDCNCSFIDRWALWRENLT +EL LAGIICWANWND+NQR NG  VADVHT+SDW
Sbjct: 1   MMGLGVDCNCSFIDRWALWRENLTLEELLLAGIICWANWNDRNQRNNGIDVADVHTESDW 60

Query: 98  ITNYAMELLTRRRKTNEAQKNLP------------------------------------- 157
           ITNYAMELLTRRRKTNEAQKNLP                                     
Sbjct: 61  ITNYAMELLTRRRKTNEAQKNLPCLAIWKPPSKGTVKLNVDAAFDVRNQRGGVGVLVRDD 120

Query: 158 ------SLISSHNVNSPLLVEICAIREVVCLAERCIHVGREANKPTHFLMGEALHQNNPV 181
                 +LISSHNVNSPLL EICAIRE V LAERCIHVGRE NKP HFL GEALHQN+ V
Sbjct: 121 RNSILAALISSHNVNSPLLAEICAIREAVRLAERCIHVGREGNKPAHFLAGEALHQNHLV 180

[Close]

BLAST of Moc04g07150 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 7.3e-13
Identity = 36/97 (37.11%), Postives = 52/97 (53.61%), Query Frame = 0


Query: 5    PVCPICLEEDETTDHTLVGCNKAGQIWDISLPMVMGLGVDCNCSFIDRWALWRENLTPKE 64
            P C IC +  E+  H    C +A QIW    P +  L  + N SF++ W+   E L PK+
Sbjct: 1171 PACTICGDRRESIIHAFFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKD 1230

Query: 65   LPLAGIICWANWNDKNQRKNGEVVADVHTKSDWITNY 102
            L LA I  W  WND+N   +G+ V+ V  K +W+T +
Sbjct: 1231 LNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPF 1267

[Close]

BLAST of Moc04g07150 vs. ExPASy TrEMBL
Match: A0A6J1CTE3 (uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014578 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.2e-10
Identity = 37/108 (34.26%), Postives = 54/108 (50.00%), Query Frame = 0


Query: 1   MDVFPVCPICLEEDETTDHTLVGCNKAGQIWDISLPMVMGLGVDCNCSFIDRWALWRENL 60
           +D  P C +C ++ ETTDH L  C +A ++W+I LP       D N S  D      E+L
Sbjct: 22  IDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAFP-QCDFNNSVQDGLLYLVESL 81

Query: 61  TPKELPLAGIICWANWNDKNQRKNGEVVADVHTKSDWITNYAMELLTR 109
           +  +  L G+  WA WND+N  +    + D   +SDWI  Y  +   R
Sbjct: 82  STPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILTYVRDFQVR 128

[Close]

BLAST of Moc04g07150 vs. ExPASy TrEMBL
Match: A0A5A7VQ24 (Adenylate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00510 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 4.4e-10
Identity = 50/171 (29.24%), Postives = 79/171 (46.20%), Query Frame = 0


Query: 1   MDVFPVCPICLEEDETTDHTLVGCNKAGQIWDISLPMVMGLGVDCNCSFIDRWALWRENL 60
           MD+ P+CP C +  ET    + GC  A ++W   LP+V+  G +    F D   +  ++L
Sbjct: 140 MDISPLCPYCGKHPETITQVVFGCRLAKELWKCFLPVVLS-GRNYYFDFSDFCVVLADSL 199

Query: 61  TPKELPLAGIICWANWNDKNQRKNGEVVADVHTKSDWITNYAMELLTRRRKTNEAQKNL- 120
           +P +  L  I CW  W+D N  +   VV DV  K  W+++Y    +     T+    N+ 
Sbjct: 200 SPLDFSLWCIGCWGLWDDHNDFRVCGVVLDVDAKCWWLSSY----VRGNGSTSSPGWNIW 259

Query: 121 ---------------PSLISSHNVNSPLLVEICAIREVVCLAERCIHVGRE 156
                           +     ++NSPLL+E  AI+E + LA    H+G E
Sbjct: 260 GVGVVNVDANGDVLGATAKQFSSLNSPLLLETLAIKEGISLAS-APHLGDE 304

[Close]

BLAST of Moc04g07150 vs. ExPASy TrEMBL
Match: A0A6J1DPU1 (uncharacterized protein LOC111022426 OS=Momordica charantia OX=3673 GN=LOC111022426 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 6.6e-06
Identity = 44/140 (31.43%), Postives = 64/140 (45.71%), Query Frame = 0


Query: 29  QIWDISLPMVMGLGVDCNCSFIDRWALWRENLTPKELPLAGIICWANWNDKNQRKNGEVV 88
           QIW   LP    LG   N  +++RW+ W +NL+   L LA I CWA WND N   N + +
Sbjct: 58  QIWRAVLPEA-ELGSLFNDCYLERWSYWMKNLSASNLRLAEITCWALWNDHNALINKKKI 117

Query: 89  ADVHTK------SDW---ITNYAMELLTRRRKTNEAQ-----------KNLPSLISSHNV 148
            +   K      + W        +++ T    + E             + + ++I  H V
Sbjct: 118 PEAPIKRRIPRVNAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAMIDFHVV 177

[Close]

BLAST of Moc04g07150 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 41.6 bits (96), Expect = 8.0e-04
Identity = 25/82 (30.49%), Postives = 35/82 (42.68%), Query Frame = 0


Query: 5   PVCPICLEEDETTDHTLVGCNKAGQIWDIS-LP----MVMGLGVDCNCSFIDRWALWREN 64
           P C  C +EDET+ H    C  A Q+W  S +P       G+ ++     +    L   N
Sbjct: 54  PQCHRCCQEDETSQHLFFDCFYAQQVWRASGIPHQELRTTGITMETKMELLLSSCL--AN 113

Query: 65  LTPKELPLAGIICWANWNDKNQ 82
             P+   LA  I W  W  +NQ
Sbjct: 114 RQPQLFNLAIWILWRLWKSRNQ 133

The following BLAST results are available for this feature:

BLAST of Moc04g07150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141766.1	7.9e-62	67.20	uncharacterized protein LOC111012047 [Momordica charantia]	[more]
XP_022158377.1	1.5e-12	37.11	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
XP_022145060.1	2.4e-10	34.26	uncharacterized protein LOC111014578 [Momordica charantia]	[more]
KAA0067681.1	9.1e-10	29.24	UMP-CMP kinase isoform X1 [Cucumis melo var. makuwa] >TYK23685.1 UMP-CMP kinase ...	[more]
KAF7823736.1	1.0e-05	38.37	putative ribonuclease H-like domain-containing protein [Senna tora]	[more]

BLAST of Moc04g07150 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc04g07150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CK80	3.8e-62	67.20	uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1DX30	7.3e-13	37.11	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A6J1CTE3	1.2e-10	34.26	uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A5A7VQ24	4.4e-10	29.24	Adenylate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G005...	[more]
A0A6J1DPU1	6.6e-06	31.43	uncharacterized protein LOC111022426 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]

BLAST of Moc04g07150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25270.1	8.0e-04	30.49	Ribonuclease H-like superfamily protein	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g07150.1	Moc04g07150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006139	nucleobase-containing compound metabolic process
molecular_function	GO:0016301	kinase activity