Homology
BLAST of Moc04g04430 vs. NCBI nr
Match:
XP_022135680.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])
HSP 1 Score: 705.3 bits (1819), Expect = 2.9e-199
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ
Sbjct: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP 120
LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP
Sbjct: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP 120
Query: 121 TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAI 180
TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAI
Sbjct: 121 TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAI 180
Query: 181 LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG 240
LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG
Sbjct: 181 LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG 240
Query: 241 DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS 300
DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS
Sbjct: 241 DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS 300
Query: 301 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG 352
DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG
Sbjct: 301 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG 351
BLAST of Moc04g04430 vs. NCBI nr
Match:
XP_022135713.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])
HSP 1 Score: 524.6 bits (1350), Expect = 7.0e-145
Identity = 256/354 (72.32%), Postives = 303/354 (85.59%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
M++ +ES SKK EE+ EHARV++W YIFGF E+A++KCAIELKIA+ IE+ SPMTLS+
Sbjct: 11 MDLALKESTSKK-EEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSK 70
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSSAL+CSPPLL+RILRFL RGIF+E+TT +NLTGY+ TPLSRLL + SMAPFLLL
Sbjct: 71 LSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLL 130
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMIT 180
ES+P MLAPW LS +K +G PFEA HG D+WSY A D H+ + N AMAC+ R+IT
Sbjct: 131 ESNPVMLAPWHNLSALIKAKDG-TPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVIT 190
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILEGC EIFEGVG +VDVGGGNGT+LSMIVKA PWI+GINFDLPHV+SVAQEC+GVQ
Sbjct: 191 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQ 250
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G+MFD IPKADAA +MWVLHDW+DE+CIKILKNC+DAIP+ GKVIIV+ VI+ EE
Sbjct: 251 HVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEE 310
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQ 351
NNL DVGL+LDMVMMAHTNKGKERT +EWAYVLH+AGF+RYTITP++AVQS+IQ
Sbjct: 311 NNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQ 362
BLAST of Moc04g04430 vs. NCBI nr
Match:
XP_022136086.1 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica charantia])
HSP 1 Score: 522.3 bits (1344), Expect = 3.5e-144
Identity = 259/354 (73.16%), Postives = 301/354 (85.03%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
M++ +ES SKKEE+ E+ A V++W YIFGF +MA++KCAIELKIA+ IE+ SPMTLSQ
Sbjct: 1 MDLALKESTSKKEEDHEQ-AHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQ 60
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSSAL+CSPPLL+RILRFL RGIF+E+TT +NLTGY+ TPLSRLL + SMAP LLL
Sbjct: 61 LSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMIT 180
ESSP MLA W LS +K +G PFEA HG D+WSY A D H+ + N AMAC+ R+IT
Sbjct: 121 ESSPIMLASWHNLSARIKASDG-TPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVIT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILEGC EIFEGVG +VDVGGGNGT+LSMIVKA PWIRGINFDLPHV+SVAQECMGVQ
Sbjct: 181 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G+MFD IPKADAA LM VLHDW+DE+CIKILKNCKDA+P+ TGKVIIV+TVI+ EE
Sbjct: 241 HVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQ 351
NLSDVGLMLDMVMMA TNKGKERTC+EWA+VLH++GFSRYTITPIRA+QS+IQ
Sbjct: 301 KNLSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQ 352
BLAST of Moc04g04430 vs. NCBI nr
Match:
XP_038888665.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])
HSP 1 Score: 514.2 bits (1323), Expect = 9.4e-142
Identity = 259/355 (72.96%), Postives = 294/355 (82.82%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
M+ +ESI KKEEE+E+ ARV+IW YIFGFVE+A+VKCAIELKIA+ IET S MTLSQ
Sbjct: 1 MDFAFKESIPKKEEEDEQ-ARVQIWNYIFGFVEIAIVKCAIELKIADTIETHGSSMTLSQ 60
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LS AL+CS LL+RILRFLV RGIF+++ T ENLT YS TPLSRLL + SM P +LL
Sbjct: 61 LSLALNCSSSLLYRILRFLVNRGIFKQEITEENLTSYSQTPLSRLLASSNNNSMVPLVLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMIT 180
ESSP MLAPW +LS +K G PFEAAHG D+WS+AA D +H+ +IN+AM+C+ R++T
Sbjct: 121 ESSPVMLAPWHRLSDRIKANEG-TPFEAAHGKDLWSFAAADPIHNTIINEAMSCTARVLT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILE C EIFEG+G +VDVGGGNGT LSMIVKA PWI+GIN DLPHVIS +QE GVQ
Sbjct: 181 VPAILEDCPEIFEGIGSLVDVGGGNGTCLSMIVKACPWIKGINLDLPHVISSSQEYTGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G M DSIPKADAA MWVLHDWDDE CIKILKNC+DAIP TGKVIIV+ VI EKEE
Sbjct: 241 HVSGSMLDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG 352
NNLSDVGLMLDMVMMAHTN GKERT KEW YVLHQAGF+RYTITPIRAVQS+IQG
Sbjct: 301 NNLSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITPIRAVQSVIQG 353
BLAST of Moc04g04430 vs. NCBI nr
Match:
XP_023554119.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 512.3 bits (1318), Expect = 3.6e-141
Identity = 253/354 (71.47%), Postives = 298/354 (84.18%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
M+ ++SISKK EE EHAR+++W+YIFGFVEMA++KCAIELKI + I++ MTL +
Sbjct: 1 MDFALKQSISKK--EEHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPE 60
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSS+L+CS LLHRI+RFLV RGIF+++ T ENLT YSHTPLSRLLT+ SMAPF+LL
Sbjct: 61 LSSSLNCSSSLLHRIMRFLVHRGIFKQEITGENLTRYSHTPLSRLLTSGSENSMAPFVLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMIT 180
ES+P MLAPW LS +K GN PFEAAHG D+WSYAA D +H+A+ N AMACS R+++
Sbjct: 121 ESNPVMLAPWHSLSARIK-GNEAIPFEAAHGKDIWSYAAADPIHNAMFNDAMACSARVMS 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILE C EIFEGV C+VDVGGGNGT+LSMIVKA PWI+GINFDLPHVI+ +GVQ
Sbjct: 181 VPAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVIASLPADIGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HVGG+MFD IPKADAA LMWVLHDWDDE CIKILKNC+DAIP+ TGKVIIV+ V+ K+E
Sbjct: 241 HVGGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQ 351
NNLSDVGLMLDMVMMAHTN GKERT KEWAYVLHQAGF+R+TITPIRA+QS+IQ
Sbjct: 301 NNLSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQ 351
BLAST of Moc04g04430 vs. ExPASy Swiss-Prot
Match:
Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)
HSP 1 Score: 362.8 bits (930), Expect = 4.6e-99
Identity = 193/369 (52.30%), Postives = 252/369 (68.29%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTS--PMTL 60
++ + E +K +EE A + IWKY+FGF ++A KCAI+LKI EAIE S P+TL
Sbjct: 10 LDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTL 69
Query: 61 SQLSSALSCSPPLLHRILRFLVRRGIFREQTTTENL-TGYSHTPLSRLLT------TSMA 120
++LSSA+S SP L RI+RFLV +GIF+E T + L TGY +TPLSR L S+A
Sbjct: 70 AELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLA 129
Query: 121 PFLLLESSPTMLAPWQKLSTCVK---EGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAM 180
PF+L E++P MLAPW +LS+ V G+ PF+A HG DVWS+A + S +IN+AM
Sbjct: 130 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 189
Query: 181 ACSGRMITVPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISV 240
AC R + VP + C +F+GV +VDVGGG G + M+VK +PWI+G NFDLPHVI V
Sbjct: 190 ACDARRV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEV 249
Query: 241 AQECMGVQHVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVD 300
A+ GV++V GDMFDSIP DA + WVLHDW D++CIKILKNCK+A+P GKV+IV+
Sbjct: 250 AEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVE 309
Query: 301 TVINE--------KEENNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTIT 350
+VI E + + L V LMLDMVMMAHT+ GKERT KEW +VL +AGF+RY +
Sbjct: 310 SVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVR 369
BLAST of Moc04g04430 vs. ExPASy Swiss-Prot
Match:
Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)
HSP 1 Score: 264.6 bits (675), Expect = 1.7e-69
Identity = 149/350 (42.57%), Postives = 212/350 (60.57%), Query Frame = 0
Query: 15 EEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQLSSALSCSPP---- 74
EE + +IW+++F FV+ +KCA+EL I + I + P+T+S++ +L + P
Sbjct: 5 EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64
Query: 75 ---LLHRILRFLVRRGIFREQTTTE-NLTGYSHTPLSRLLTT----SMAPFLLLESSPTM 134
L RI+R LV + +F + E N Y+ T S+ L +++P +L E++P +
Sbjct: 65 NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124
Query: 135 LAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAILE 194
L PWQ L C +E + +PFE AHG ++W A D + +N AM CS I +LE
Sbjct: 125 LKPWQYLGKCAQEKS--SPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLE 184
Query: 195 GCKEIFEGV-GCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGD 254
K+ F G+ G +VDVGGG G+ ++ IVKA P I+GINFDLPHV++ A E GV+HVGGD
Sbjct: 185 -YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGD 244
Query: 255 MFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAI-PRTTGKVIIVDTVINEKEENNLS 314
MF IP+ADA ++ W+LHDW DE+C ILKNC AI + GKVIIVD V+ +
Sbjct: 245 MFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFD 304
Query: 315 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQ 351
+GL+ D++MMAHT GKERT EW +L+ AGF RY + A II+
Sbjct: 305 KMGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIE 351
BLAST of Moc04g04430 vs. ExPASy Swiss-Prot
Match:
B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)
HSP 1 Score: 258.5 bits (659), Expect = 1.2e-67
Identity = 155/370 (41.89%), Postives = 224/370 (60.54%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
ME+ R + + E +WK I G + V+KCA+EL+I + + + ++P+TL+Q
Sbjct: 1 MELARND-----QTEAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQ 60
Query: 61 LSSALSCSPPL----LHRILRFLVRRGIFREQTT---TENLTGYSHTPLSRLLTT----- 120
++S++ SP L L RI+R LVRR IF + + E L G +H+ SRLL +
Sbjct: 61 IASSVPDSPSLNLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHS--SRLLLSKTTLP 120
Query: 121 ---SMAPFLLLESSPTMLAPWQKLSTCVKE-GNGIAPFEAAH-GMDVWSYAAGDAVHSAL 180
++APF+ + P + APW L+ CVKE GNG FE H G +W + G+ + +
Sbjct: 121 DQVTLAPFVAFMTHPYLSAPWSCLARCVKEGGNG---FEMVHGGRQLWDLSPGNPEFNKV 180
Query: 181 INQAMACSGRMITVPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLP 240
N MA + R IT AIL +++F G+ +VDVGG G ++S IVK+ P I+GIN+DLP
Sbjct: 181 FNDGMASTAR-ITTMAILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLP 240
Query: 241 HVISVAQECMG-VQHVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTG 300
HV++ A G V HVGG+MF+ IP A A + W+LHDW DE+C+KILKNC+ A+P G
Sbjct: 241 HVVATAPTYTGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGG 300
Query: 301 KVIIVDTVINEKEENNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPI 353
K+IIVD V+ + D +MLD+ +MA T +GKERT KEW VL + GF RY I I
Sbjct: 301 KIIIVDIVLEPEGNGLFDDAAVMLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKI 358
BLAST of Moc04g04430 vs. ExPASy Swiss-Prot
Match:
Q9LEL5 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)
HSP 1 Score: 250.4 bits (638), Expect = 3.3e-65
Identity = 136/346 (39.31%), Postives = 212/346 (61.27%), Query Frame = 0
Query: 12 KEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQLSSALSCSP-- 71
K++ + A+ +WK I+GF + V++CA+EL I + I+ PM L+ L+S L S
Sbjct: 6 KDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVN 65
Query: 72 -PLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLT----TSMAPFLLLESSPTMLA 131
L+RILR+LV+ I R + + + Y+ P++ LL+ SM P +L + +
Sbjct: 66 CDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMT 125
Query: 132 PWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAILEGC 191
PW + + + NG A FE A GM +W Y G S L N+ MA R++T +++ G
Sbjct: 126 PWHSMKDGLSD-NGTA-FEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLT-SSLISGS 185
Query: 192 KEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFD 251
+++F+G+ +VDVGGGNGT + I A+P I+ FDLPHVI+ + + ++ +GGDMF
Sbjct: 186 RDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFK 245
Query: 252 SIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLSDVGL 311
S+P A A +L +LHDW+DE+ IKILK C++A+P+ GKVIIVD ++E+ ++ LS L
Sbjct: 246 SVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRL 305
Query: 312 MLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQ 351
+LD+ M+ +T GKERT + W ++ AGFS I I A+QS+I+
Sbjct: 306 ILDIDMLVNTG-GKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIE 347
BLAST of Moc04g04430 vs. ExPASy Swiss-Prot
Match:
Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)
HSP 1 Score: 249.6 bits (636), Expect = 5.7e-65
Identity = 131/338 (38.76%), Postives = 206/338 (60.95%), Query Frame = 0
Query: 21 RVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQLSSAL---SCSPPLLHRILR 80
+ ++W I+GF + V++CA+E+ IA+ I+ +TL+QL++ L + S L+R++R
Sbjct: 20 QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVR 79
Query: 81 FLVRRGIFREQTTTENLTG-YSHTPLSRLL----TTSMAPFLLLESSPTMLAPWQKLSTC 140
+LV I ++T + YS P+ LL SM P +L + + W +
Sbjct: 80 YLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEG 139
Query: 141 VKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAILEGCKEIFEGVG 200
+ G+ A FE GMD+W Y G+ S L N+ MA R++T ++E C++ F+G+
Sbjct: 140 LGNGSTTA-FEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT-KTLIEDCRDTFQGLD 199
Query: 201 CVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFDSIPKADAA 260
+VD+GGGNGT + I +A+P I+ +DLPHV++ + + ++ V GDMF S+P A A
Sbjct: 200 SLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAI 259
Query: 261 LLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLSDVGLMLDMVMMA 320
LL +LHDW DEEC+ ILK CK+AIP+ TGKVIIVD + E+ + L+ L+LD+ M+
Sbjct: 260 LLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLV 319
Query: 321 HTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQ 351
+T G+ERT +W +L +AGF + I PIRA+QS+I+
Sbjct: 320 NTG-GRERTADDWENLLKRAGFRSHKIRPIRAIQSVIE 354
BLAST of Moc04g04430 vs. ExPASy TrEMBL
Match:
A0A6J1C1Q0 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007578 PE=3 SV=1)
HSP 1 Score: 705.3 bits (1819), Expect = 1.4e-199
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ
Sbjct: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP 120
LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP
Sbjct: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTTSMAPFLLLESSP 120
Query: 121 TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAI 180
TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAI
Sbjct: 121 TMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAI 180
Query: 181 LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG 240
LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG
Sbjct: 181 LEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGG 240
Query: 241 DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS 300
DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS
Sbjct: 241 DMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLS 300
Query: 301 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG 352
DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG
Sbjct: 301 DVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG 351
BLAST of Moc04g04430 vs. ExPASy TrEMBL
Match:
A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)
HSP 1 Score: 524.6 bits (1350), Expect = 3.4e-145
Identity = 256/354 (72.32%), Postives = 303/354 (85.59%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
M++ +ES SKK EE+ EHARV++W YIFGF E+A++KCAIELKIA+ IE+ SPMTLS+
Sbjct: 11 MDLALKESTSKK-EEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSK 70
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSSAL+CSPPLL+RILRFL RGIF+E+TT +NLTGY+ TPLSRLL + SMAPFLLL
Sbjct: 71 LSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLL 130
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMIT 180
ES+P MLAPW LS +K +G PFEA HG D+WSY A D H+ + N AMAC+ R+IT
Sbjct: 131 ESNPVMLAPWHNLSALIKAKDG-TPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVIT 190
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILEGC EIFEGVG +VDVGGGNGT+LSMIVKA PWI+GINFDLPHV+SVAQEC+GVQ
Sbjct: 191 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQ 250
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G+MFD IPKADAA +MWVLHDW+DE+CIKILKNC+DAIP+ GKVIIV+ VI+ EE
Sbjct: 251 HVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEE 310
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQ 351
NNL DVGL+LDMVMMAHTNKGKERT +EWAYVLH+AGF+RYTITP++AVQS+IQ
Sbjct: 311 NNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQ 362
BLAST of Moc04g04430 vs. ExPASy TrEMBL
Match:
A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)
HSP 1 Score: 522.3 bits (1344), Expect = 1.7e-144
Identity = 259/354 (73.16%), Postives = 301/354 (85.03%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
M++ +ES SKKEE+ E+ A V++W YIFGF +MA++KCAIELKIA+ IE+ SPMTLSQ
Sbjct: 1 MDLALKESTSKKEEDHEQ-AHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQ 60
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LSSAL+CSPPLL+RILRFL RGIF+E+TT +NLTGY+ TPLSRLL + SMAP LLL
Sbjct: 61 LSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMIT 180
ESSP MLA W LS +K +G PFEA HG D+WSY A D H+ + N AMAC+ R+IT
Sbjct: 121 ESSPIMLASWHNLSARIKASDG-TPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVIT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILEGC EIFEGVG +VDVGGGNGT+LSMIVKA PWIRGINFDLPHV+SVAQECMGVQ
Sbjct: 181 VPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HV G+MFD IPKADAA LM VLHDW+DE+CIKILKNCKDA+P+ TGKVIIV+TVI+ EE
Sbjct: 241 HVEGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQ 351
NLSDVGLMLDMVMMA TNKGKERTC+EWA+VLH++GFSRYTITPIRA+QS+IQ
Sbjct: 301 KNLSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQ 352
BLAST of Moc04g04430 vs. ExPASy TrEMBL
Match:
A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)
HSP 1 Score: 500.4 bits (1287), Expect = 6.8e-138
Identity = 249/354 (70.34%), Postives = 295/354 (83.33%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
M+ ++SISKK EE EHAR+++W+YIFGFVEMA++KCAIELKI + I++ MTL +
Sbjct: 1 MDFALKQSISKK--EEHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPE 60
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLT----TSMAPFLLL 120
LSS+L+CS LL+RI+RFLV RGIF+E+ T ENLT YSHTPLSRLL +SMAP LLL
Sbjct: 61 LSSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMIT 180
ES+P MLAPW LS +K GN PFEAAHG D+WSYAA + H+A+ N AMACS R++T
Sbjct: 121 ESNPVMLAPWHGLSARIK-GNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILE C EIFEGV C+VDVGGGNGT+LSMIVKA PWI+GINFDLPHV++ + +GVQ
Sbjct: 181 VPAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQ 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HVGG+MFD IPKADAA LMWVLH WDDE CIKILK C++AIP+ TGKVIIV+ V+ EKEE
Sbjct: 241 HVGGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEE 300
Query: 301 NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQ 351
NNLSDVGLMLDMVMMAHTN GKERT +EWAYVLHQAGF+R+TITPIRA+QS+IQ
Sbjct: 301 NNLSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQ 351
BLAST of Moc04g04430 vs. ExPASy TrEMBL
Match:
A0A5D3DJ08 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004240 PE=3 SV=1)
HSP 1 Score: 498.0 bits (1281), Expect = 3.4e-137
Identity = 253/360 (70.28%), Postives = 293/360 (81.39%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTSPMTLSQ 60
M + SISKKEEEE ARV+IWKYIFGFVEMA+VKCAIEL+I + IE+ SPMTLSQ
Sbjct: 1 MNFAPQVSISKKEEEE---ARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQ 60
Query: 61 LSSALSCSPPLLHRILRFLVRRGIFREQTTTENLTGYSHTPLSRLLTT----SMAPFLLL 120
LS+AL+CS LL+RILRFLVRRGIF+++ T EN+ Y HTPLSRLL + SMAP LLL
Sbjct: 61 LSTALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLL 120
Query: 121 ESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMIT 180
ESSP MLAPW +LS +K GNG PFEAAHG DVWS+AA D +H+ +IN AM+C+ R+ T
Sbjct: 121 ESSPVMLAPWHRLSARIK-GNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHT 180
Query: 181 VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQ 240
VPAILE C +IFEG+G +VDVGGGNGT LSMIVKA+PWI+GINFDLPHVIS +Q+ +GV+
Sbjct: 181 VPAILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVE 240
Query: 241 HVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEE 300
HVGG+M DSIPKADAA +MWVLHDWDDE CIKILKNCK+AI GKVIIV+ +I E+ E
Sbjct: 241 HVGGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKEAISEKRGKVIIVEAIIEERSE 300
Query: 301 -----NNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSIIQG 352
NNL DVGLMLDMVMMAHT GKERT KEW VLHQAGF+RYTITPIRAV S+IQG
Sbjct: 301 EENNNNNLGDVGLMLDMVMMAHTENGKERTIKEWGNVLHQAGFTRYTITPIRAVHSVIQG 356
BLAST of Moc04g04430 vs. TAIR 10
Match:
AT4G35160.1 (O-methyltransferase family protein )
HSP 1 Score: 362.8 bits (930), Expect = 3.3e-100
Identity = 193/369 (52.30%), Postives = 252/369 (68.29%), Query Frame = 0
Query: 1 MEMTRRESISKKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTS--PMTL 60
++ + E +K +EE A + IWKY+FGF ++A KCAI+LKI EAIE S P+TL
Sbjct: 10 LDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTL 69
Query: 61 SQLSSALSCSPPLLHRILRFLVRRGIFREQTTTENL-TGYSHTPLSRLLT------TSMA 120
++LSSA+S SP L RI+RFLV +GIF+E T + L TGY +TPLSR L S+A
Sbjct: 70 AELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLA 129
Query: 121 PFLLLESSPTMLAPWQKLSTCVK---EGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAM 180
PF+L E++P MLAPW +LS+ V G+ PF+A HG DVWS+A + S +IN+AM
Sbjct: 130 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 189
Query: 181 ACSGRMITVPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISV 240
AC R + VP + C +F+GV +VDVGGG G + M+VK +PWI+G NFDLPHVI V
Sbjct: 190 ACDARRV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEV 249
Query: 241 AQECMGVQHVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVD 300
A+ GV++V GDMFDSIP DA + WVLHDW D++CIKILKNCK+A+P GKV+IV+
Sbjct: 250 AEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVE 309
Query: 301 TVINE--------KEENNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTIT 350
+VI E + + L V LMLDMVMMAHT+ GKERT KEW +VL +AGF+RY +
Sbjct: 310 SVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVR 369
BLAST of Moc04g04430 vs. TAIR 10
Match:
AT4G35150.1 (O-methyltransferase family protein )
HSP 1 Score: 316.2 bits (809), Expect = 3.5e-86
Identity = 168/346 (48.55%), Postives = 222/346 (64.16%), Query Frame = 0
Query: 15 EEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIETQTS--PMTLSQLSSALSCSPPLL 74
+EE A + IW+Y+FGF ++A KCAI+LKI EAIE S P+TLS+LSSA+S SP L
Sbjct: 10 DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHL 69
Query: 75 HRILRFLVRRGIFREQTTTENL-TGYSHTPLSRLLTTSMAPFLLLESSPTMLAPWQKLST 134
RI+RFLV +G+F+E T + L TGY++TPLSR + +
Sbjct: 70 RRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMMIT---------------------- 129
Query: 135 CVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAILEGCKEIFEGV 194
HG D+W++A + HS LIN+AMAC R + VP + C+ +F+GV
Sbjct: 130 ------------KLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPRVAGACQGLFDGV 189
Query: 195 GCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFDSIPKADA 254
VVDVGGG G + ++VK +PWI+G NFDLPHVI VAQ GV++V GDMFDSIP +DA
Sbjct: 190 ATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPASDA 249
Query: 255 ALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVIIVDTVINEKE--------ENNLSDVG 314
++ WVLHDW D++CIKILKNCK+A+ GKV+IV+ VI EK+ ++ L V
Sbjct: 250 VIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKLEHVR 309
Query: 315 LMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTITPIRAVQSII 350
L LDMVMM HT+ GKERT KEW +VL +AGF+RY + VQS+I
Sbjct: 310 LQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320
BLAST of Moc04g04430 vs. TAIR 10
Match:
AT5G54160.1 (O-methyltransferase 1 )
HSP 1 Score: 190.7 bits (483), Expect = 2.2e-48
Identity = 116/314 (36.94%), Postives = 174/314 (55.41%), Query Frame = 0
Query: 37 VKCAIELKIAEAIETQTSPMTLSQLSSALSC----SPPLLHRILRFLVRRGIFREQTTTE 96
+K A+EL + E + SPM+ ++++S L +P +L RILR L + +
Sbjct: 36 LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVL--TCSNR 95
Query: 97 NLTG------YSHTPLSRLLT-----TSMAPFLLLESSPTMLAPWQKLSTCVKEGNGIAP 156
L+G Y P+ + LT S+A L+ ++ W L + +G P
Sbjct: 96 KLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGG--IP 155
Query: 157 FEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAILEGCKEIFEGVGCVVDVGGGN 216
F A+GM + Y D + + N M+ + IT+ ILE K FEG+ +VDVGGG
Sbjct: 156 FNKAYGMSAFEYHGTDPRFNKVFNNGMS-NHSTITMKKILETYKG-FEGLTSLVDVGGGI 215
Query: 217 GTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFDSIPKADAALLMWVLHDW 276
G L MIV +P ++GINFDLPHVI A G++HVGGDMF S+PK DA + W+ HDW
Sbjct: 216 GATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDW 275
Query: 277 DDEECIKILKNCKDAIPRTTGKVIIVDTVINEKEENNLSDVGLM-LDMVMMAHTNKGKER 335
DE C+K LKNC +++P GKVI+ + ++ E +++LS ++ +D +M+AH GKER
Sbjct: 276 SDEHCVKFLKNCYESLPE-DGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKER 335
BLAST of Moc04g04430 vs. TAIR 10
Match:
AT5G53810.1 (O-methyltransferase family protein )
HSP 1 Score: 174.5 bits (441), Expect = 1.7e-43
Identity = 124/356 (34.83%), Postives = 179/356 (50.28%), Query Frame = 0
Query: 11 KKEEEEEEHARVKIWKYIFGFVEMAVVKCAIELKIAEAIET------QTSPMTLSQLSSA 70
K+E+E EE AR+ + V+K A+EL + + I T SP ++
Sbjct: 16 KEEQEVEEEARLLARRLANAAASPMVLKAALELGVIDTITTVGGGDLWLSPSEIALRLPT 75
Query: 71 LSC---SPPLLHRILRFLVRRGIFREQTTTE--NLTG-----YSHTPLSRLL-------T 130
C +P LL R+LRFLV + + +T E TG Y+ P+ + L +
Sbjct: 76 KPCNLEAPALLDRMLRFLVSHSVLKCRTVIEENGQTGKVERVYAAEPVCKYLLNKSDDVS 135
Query: 131 TSMAPFLLLESSPTMLAPWQKLSTCVKEGNGIAPFEAAHGMDVWSYAAGDAVHSALINQA 190
S A +L+ S + W L + EG F +AHGM ++ Y D + N+A
Sbjct: 136 GSFASLFMLDLSDVFIKTWTHLEDVILEGRD--AFSSAHGMKLFEYIQADERFGKVFNRA 195
Query: 191 MACSGRMIT--VPAILEGCKEIFEGVGCVVDVGGGNGTNLSMIVKAWPWIRGINFDLPHV 250
M S M+T V EG F+ V +VDVGGG G L +I +P + GINFDL V
Sbjct: 196 MLESSTMVTEKVLKFYEG----FKDVKTLVDVGGGLGNTLGLITSKYPHLIGINFDLAPV 255
Query: 251 ISVAQECMGVQHVGGDMFDSIPKADAALLMWVLHDWDDEECIKILKNCKDAIPRTTGKVI 310
++ A GV HV GDMF IPK DA + W+LHDW DE+C+ ILKNC ++ GK+I
Sbjct: 256 LANAHSYPGVNHVAGDMFIKIPKGDAIFMKWILHDWTDEQCVAILKNCWKSL-EENGKLI 315
Query: 311 IVD--TVINEKEENNLSDVGLMLDMVMMAHTNKGKERTCKEWAYVLHQAGFSRYTI 340
IV+ T + K + S++ +DM M+ + GKER E+ + + +GFSR I
Sbjct: 316 IVEMVTPVEAKSGDICSNIVFGMDMTMLTQCSGGKERDLYEFENLAYASGFSRCAI 364
BLAST of Moc04g04430 vs. TAIR 10
Match:
AT1G51990.1 (O-methyltransferase family protein )
HSP 1 Score: 174.1 bits (440), Expect = 2.2e-43
Identity = 110/318 (34.59%), Postives = 183/318 (57.55%), Query Frame = 0
Query: 36 VVKCAIELKI------AEAIETQTSPMTLSQLSSALS-CSPPLLHRILRFLVRRGI---- 95
+VK A EL + A + + SP+ L+ +++ + +P ++ R+LRFLV +
Sbjct: 31 IVKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTCK 90
Query: 96 -FREQTTTENLTGYSHTPLSRLLTT-----SMAPFLLLESSPTMLAPWQKLSTCVKEGNG 155
+++ E+ Y + + L S+AP++L + W L+ ++EG G
Sbjct: 91 LVKDEEGRES-RAYGLGKVGKKLIKDEDGFSIAPYVLAGCTKAKGGVWSYLTEAIQEG-G 150
Query: 156 IAPFEAAHGMDVWSYAAGDAVHSALINQAMACSGRMITVPAILEGCKEIFEGVGCVVDVG 215
+ +E A+ ++ Y + + N++M + I + ILE FEGV VDVG
Sbjct: 151 ASAWERANEALIFEYMKKNENLKKIFNESMT-NHTSIVMKKILENYIG-FEGVSDFVDVG 210
Query: 216 GGNGTNLSMIVKAWPWIRGINFDLPHVISVAQECMGVQHVGGDMFDSIPKADAALLMWVL 275
G G+NL+ I+ +P I+GINFDLPH++ A + GV+H+GGDMFD IP+ + L+ W+L
Sbjct: 211 GSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWIL 270
Query: 276 HDWDDEECIKILKNCKDAIPRTTGKVIIVDTVI-NEKEENNLSDV-GLMLDMVMMAHTNK 335
HDW+DE+C++ILKNCK A+P TG++I+++ ++ E E +L+ L D+ MM+ T+
Sbjct: 271 HDWNDEKCVEILKNCKKALPE-TGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSG 330
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022135680.1 | 2.9e-199 | 100.00 | (R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia] | [more] |
XP_022135713.1 | 7.0e-145 | 72.32 | (R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia] | [more] |
XP_022136086.1 | 3.5e-144 | 73.16 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica char... | [more] |
XP_038888665.1 | 9.4e-142 | 72.96 | acetylserotonin O-methyltransferase-like [Benincasa hispida] | [more] |
XP_023554119.1 | 3.6e-141 | 71.47 | (RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q9T003 | 4.6e-99 | 52.30 | Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1... | [more] |
Q6WUC2 | 1.7e-69 | 42.57 | (R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=... | [more] |
B0ZB56 | 1.2e-67 | 41.89 | Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1 | [more] |
Q9LEL5 | 3.3e-65 | 39.31 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=... | [more] |
Q7XB10 | 5.7e-65 | 38.76 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferu... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1C1Q0 | 1.4e-199 | 100.00 | (R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO... | [more] |
A0A6J1C5N1 | 3.4e-145 | 72.32 | (R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO... | [more] |
A0A6J1C2I8 | 1.7e-144 | 73.16 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica ch... | [more] |
A0A6J1GLX2 | 6.8e-138 | 70.34 | (RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L... | [more] |
A0A5D3DJ08 | 3.4e-137 | 70.28 | (R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=11946... | [more] |