Moc04g02580 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g02580
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionS-type anion channel SLAH1-like
Locationchr4: 1641692 .. 1642762 (+)
RNA-Seq ExpressionMoc04g02580
SyntenyMoc04g02580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAAATGGATCAGAATCAAAAGAAAGAAGCTTCATTTTTCCTCCTCCTCTTAGCTAAATTCCACGCAGGCTACTTCAGAATCAGCATGTCCCTCTGCGCCCAAGCCCTGTTATGGAAAATCCTCAAACACCCAATTCAAAACCAAAATTCTCTCACTCGAATCCTCCAATTCCTACCCAATACAGCTTTCTTGTTCCTATGGTCATTGGCACTCTTAATTCTGCTTTCCCTTTCGCTCACATACATTTTAAGATGCTTCTTCCACTTCAAATTGGTAAAAGCAGAGTTTTTGAACAGAGTGGGTGTGAATTACCTCTTTGCTCCATGGATCTCTTGGCTCCTCTTGCTTCAATCCTCACCTCTAAAGTTCGTTCCCCAAGAAATCCTCATGTGGGTGTTCGTGATCCCGATTGCGGCATTGGACGTGAAGATCTATGGCCAGTGGTTCACGAAAGGGAAGAGATTTCTGTCCACCGTGGCGAATCCCACGAGCCAGCTGTCGGTGATTGGAAACTTGGCCGGAGCGCGGGCCGCGGCGGTGATGGGGTGGCGGGAGACGGCATTGTGCATGTTCTCCCTCGGAATGGCACATTACTTAGTCCTGTTCGTGACTCTGTATCAGAGGTTGGCCGGAAGCAACTGCCTTCCGGCCACTTTGAGGCCTGTTTTTTTCCTATTCTTCGCGGCACCGAGCATGGCGAGTGTGGCTTGGAATTCGATTAATGGGAAATTTGATGTGTTTTCAAAGATGATGTTTTTTCTCTCCGTTTTCCTGTTCGTGTCGCTGGTTTTGAGGCCGCGGCTTTTCAGGAAATCTATGAGGAAATTCAGCGTGGCGTGGTGGGCTTATTCGTTTCCTGTGTCGGTTCTTGCGTTGGCTTCCATTGAATACGCTAAGGAAGTTGGGGCCGGCGCCGGCGCGGCGCATGTTTTTGCTCTTCTTTTGTCTCTGCTCTCCGTTTTGGTGTCTCTGTTTTTGATGGCTGTTACGGTTATGAGGTCTAATTCTTTGATTCCGGCGAGCCCGCCGGAGGTTAGTTCTGACAGCAGCAGTGGAGAGGTGTGA

mRNA sequence

ATGGAGGAAATGGATCAGAATCAAAAGAAAGAAGCTTCATTTTTCCTCCTCCTCTTAGCTAAATTCCACGCAGGCTACTTCAGAATCAGCATGTCCCTCTGCGCCCAAGCCCTGTTATGGAAAATCCTCAAACACCCAATTCAAAACCAAAATTCTCTCACTCGAATCCTCCAATTCCTACCCAATACAGCTTTCTTGTTCCTATGGTCATTGGCACTCTTAATTCTGCTTTCCCTTTCGCTCACATACATTTTAAGATGCTTCTTCCACTTCAAATTGGTAAAAGCAGAGTTTTTGAACAGAGTGGGTGTGAATTACCTCTTTGCTCCATGGATCTCTTGGCTCCTCTTGCTTCAATCCTCACCTCTAAAGTTCGTTCCCCAAGAAATCCTCATGTGGGTGTTCGTGATCCCGATTGCGGCATTGGACGTGAAGATCTATGGCCAGTGGTTCACGAAAGGGAAGAGATTTCTGTCCACCGTGGCGAATCCCACGAGCCAGCTGTCGGTGATTGGAAACTTGGCCGGAGCGCGGGCCGCGGCGGTGATGGGGTGGCGGGAGACGGCATTGTGCATGTTCTCCCTCGGAATGGCACATTACTTAGTCCTGTTCGTGACTCTGTATCAGAGGTTGGCCGGAAGCAACTGCCTTCCGGCCACTTTGAGGCCTGTTTTTTTCCTATTCTTCGCGGCACCGAGCATGGCGAGTGTGGCTTGGAATTCGATTAATGGGAAATTTGATGTGTTTTCAAAGATGATGTTTTTTCTCTCCGTTTTCCTGTTCGTGTCGCTGGTTTTGAGGCCGCGGCTTTTCAGGAAATCTATGAGGAAATTCAGCGTGGCGTGGTGGGCTTATTCGTTTCCTGTGTCGGTTCTTGCGTTGGCTTCCATTGAATACGCTAAGGAAGTTGGGGCCGGCGCCGGCGCGGCGCATGTTTTTGCTCTTCTTTTGTCTCTGCTCTCCGTTTTGGTGTCTCTGTTTTTGATGGCTGTTACGGTTATGAGGTCTAATTCTTTGATTCCGGCGAGCCCGCCGGAGGTTAGTTCTGACAGCAGCAGTGGAGAGGTGTGA

Coding sequence (CDS)

ATGGAGGAAATGGATCAGAATCAAAAGAAAGAAGCTTCATTTTTCCTCCTCCTCTTAGCTAAATTCCACGCAGGCTACTTCAGAATCAGCATGTCCCTCTGCGCCCAAGCCCTGTTATGGAAAATCCTCAAACACCCAATTCAAAACCAAAATTCTCTCACTCGAATCCTCCAATTCCTACCCAATACAGCTTTCTTGTTCCTATGGTCATTGGCACTCTTAATTCTGCTTTCCCTTTCGCTCACATACATTTTAAGATGCTTCTTCCACTTCAAATTGGTAAAAGCAGAGTTTTTGAACAGAGTGGGTGTGAATTACCTCTTTGCTCCATGGATCTCTTGGCTCCTCTTGCTTCAATCCTCACCTCTAAAGTTCGTTCCCCAAGAAATCCTCATGTGGGTGTTCGTGATCCCGATTGCGGCATTGGACGTGAAGATCTATGGCCAGTGGTTCACGAAAGGGAAGAGATTTCTGTCCACCGTGGCGAATCCCACGAGCCAGCTGTCGGTGATTGGAAACTTGGCCGGAGCGCGGGCCGCGGCGGTGATGGGGTGGCGGGAGACGGCATTGTGCATGTTCTCCCTCGGAATGGCACATTACTTAGTCCTGTTCGTGACTCTGTATCAGAGGTTGGCCGGAAGCAACTGCCTTCCGGCCACTTTGAGGCCTGTTTTTTTCCTATTCTTCGCGGCACCGAGCATGGCGAGTGTGGCTTGGAATTCGATTAATGGGAAATTTGATGTGTTTTCAAAGATGATGTTTTTTCTCTCCGTTTTCCTGTTCGTGTCGCTGGTTTTGAGGCCGCGGCTTTTCAGGAAATCTATGAGGAAATTCAGCGTGGCGTGGTGGGCTTATTCGTTTCCTGTGTCGGTTCTTGCGTTGGCTTCCATTGAATACGCTAAGGAAGTTGGGGCCGGCGCCGGCGCGGCGCATGTTTTTGCTCTTCTTTTGTCTCTGCTCTCCGTTTTGGTGTCTCTGTTTTTGATGGCTGTTACGGTTATGAGGTCTAATTCTTTGATTCCGGCGAGCCCGCCGGAGGTTAGTTCTGACAGCAGCAGTGGAGAGGTGTGA

Protein sequence

MEEMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSINGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGEV
Homology
BLAST of Moc04g02580 vs. NCBI nr
Match: XP_022136183.1 (S-type anion channel SLAH1-like [Momordica charantia])

HSP 1 Score: 672.5 bits (1734), Expect = 1.9e-189
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 0

Query: 1   MEEMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFL 60
           MEEMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFL
Sbjct: 1   MEEMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFL 60

Query: 61  PNTAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQS 120
           PNTAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQS
Sbjct: 61  PNTAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQS 120

Query: 121 SPLKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAA 180
           SPLKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAA
Sbjct: 121 SPLKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAA 180

Query: 181 AVMGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWN 240
           AVMGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWN
Sbjct: 181 AVMGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWN 240

Query: 241 SINGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYA 300
           SINGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYA
Sbjct: 241 SINGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYA 300

Query: 301 KEVGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGEV 357
           KEVGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGEV
Sbjct: 301 KEVGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGEV 356

BLAST of Moc04g02580 vs. NCBI nr
Match: XP_038887173.1 (S-type anion channel SLAH1-like, partial [Benincasa hispida])

HSP 1 Score: 550.4 bits (1417), Expect = 1.1e-152
Identity = 295/351 (84.05%), Postives = 316/351 (90.03%), Query Frame = 0

Query: 6   QNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAF 65
           QN+ K+AS FL LLAKFHAGYFRISMSLC QALLWKILK PIQN+NSL +IL+ LPNTAF
Sbjct: 7   QNEIKKASIFLQLLAKFHAGYFRISMSLCGQALLWKILKQPIQNENSLRKILRLLPNTAF 66

Query: 66  LFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLK- 125
           L LWS+ L IL SLSL YILRCFFHFKLVK+EFL+RVGVNYLFAPWISWLLLLQSSP K 
Sbjct: 67  LLLWSITLFILASLSLIYILRCFFHFKLVKSEFLHRVGVNYLFAPWISWLLLLQSSPFKS 126

Query: 126 FVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMG 185
            +P +ILMW FVIPI  LDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMG
Sbjct: 127 LIPNQILMWAFVIPIVVLDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMG 186

Query: 186 WRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSING 245
           WRE+ALCMFSLGMAHYLVLFVTLYQRL+GS+ LPATLRPVFFLFFAAPSMAS+AW+SING
Sbjct: 187 WRESALCMFSLGMAHYLVLFVTLYQRLSGSDSLPATLRPVFFLFFAAPSMASLAWSSING 246

Query: 246 KFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVG 305
            FD FSKM+FFLSVFLFVSLV RP LFRKSMRKFSVAWWAYSFP+SVLALA  EYAKEV 
Sbjct: 247 GFDAFSKMLFFLSVFLFVSLVSRPGLFRKSMRKFSVAWWAYSFPLSVLALACNEYAKEV- 306

Query: 306 AGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGE 356
             A AAHVFALLL+LLSVLV LFLM VTV+RSNSLIP + PE+SSDSSS E
Sbjct: 307 -EAEAAHVFALLLALLSVLVCLFLMIVTVLRSNSLIPVTSPEISSDSSSAE 355

BLAST of Moc04g02580 vs. NCBI nr
Match: XP_008455447.1 (PREDICTED: S-type anion channel SLAH1-like [Cucumis melo])

HSP 1 Score: 542.7 bits (1397), Expect = 2.3e-150
Identity = 288/351 (82.05%), Postives = 315/351 (89.74%), Query Frame = 0

Query: 6   QNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAF 65
           QN+ K+ SFFL LLAKFHAGYFRISMSLC QALLWKILK PIQN+NSL RIL+ LPNTAF
Sbjct: 6   QNEDKKPSFFLQLLAKFHAGYFRISMSLCGQALLWKILKQPIQNENSLRRILRLLPNTAF 65

Query: 66  LFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLK- 125
           L LWSL L I  SLSL YILRCFFHFKLVK+EFL+RVGVNYLFAPW+SWLLLLQSSP K 
Sbjct: 66  LLLWSLTLFISASLSLIYILRCFFHFKLVKSEFLHRVGVNYLFAPWLSWLLLLQSSPFKS 125

Query: 126 FVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMG 185
            +P +ILMWVF+IPI  LDVKIYGQWFTKGKRFLSTVANP+SQLSVIGNLAGARAAAV+G
Sbjct: 126 LIPNQILMWVFIIPIVVLDVKIYGQWFTKGKRFLSTVANPSSQLSVIGNLAGARAAAVIG 185

Query: 186 WRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSING 245
           WRE+ALC+FSLGMAHYLVLFVTLYQRL+GSN LPA LRPVFFLFFAAPSMAS+AW+SING
Sbjct: 186 WRESALCLFSLGMAHYLVLFVTLYQRLSGSNTLPAILRPVFFLFFAAPSMASLAWSSING 245

Query: 246 KFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVG 305
            FD FSKM+FFLSVFLFVSLV RP LFRKSMRKFS+AWWAYSFP+SVLALA  EYAKEV 
Sbjct: 246 GFDTFSKMLFFLSVFLFVSLVSRPGLFRKSMRKFSIAWWAYSFPLSVLALACNEYAKEV- 305

Query: 306 AGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGE 356
             A AAHVFALLL+L+SVLVSLFLM VTV+RS+ L+P +P E+SSDSSS E
Sbjct: 306 -EATAAHVFALLLALISVLVSLFLMIVTVLRSHLLVPQTPTEISSDSSSAE 354

BLAST of Moc04g02580 vs. NCBI nr
Match: XP_004144593.2 (S-type anion channel SLAH1 [Cucumis sativus] >KAE8645739.1 hypothetical protein Csa_020155 [Cucumis sativus])

HSP 1 Score: 538.9 bits (1387), Expect = 3.3e-149
Identity = 288/351 (82.05%), Postives = 314/351 (89.46%), Query Frame = 0

Query: 6   QNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAF 65
           Q++ K+ SFFL LLAK HAGYFRISMSLC QALLWKILK PIQN+NSL RIL+ LPNTAF
Sbjct: 6   QDEVKKPSFFLQLLAKVHAGYFRISMSLCGQALLWKILKQPIQNENSLRRILRLLPNTAF 65

Query: 66  LFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLK- 125
           L LWSL L IL SLSL YILRCFFHFKLVK+EFL+RVGVNYLFAPW+SWLLLLQSSP K 
Sbjct: 66  LLLWSLTLFILASLSLIYILRCFFHFKLVKSEFLHRVGVNYLFAPWLSWLLLLQSSPFKS 125

Query: 126 FVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMG 185
            +P +ILMWVF+IPI  LDVKIYGQWFTKGKRFLSTVANP+SQLSVIGNLAGARAAAV+G
Sbjct: 126 LLPNQILMWVFIIPIVVLDVKIYGQWFTKGKRFLSTVANPSSQLSVIGNLAGARAAAVIG 185

Query: 186 WRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSING 245
           WRE+ALC+FSLGMAHYLVLFVTLYQRL+GSN LPA LRPVFFLFFAAPSMAS+AW+SING
Sbjct: 186 WRESALCLFSLGMAHYLVLFVTLYQRLSGSNTLPAILRPVFFLFFAAPSMASLAWSSING 245

Query: 246 KFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVG 305
            FD FSKM+FFLS+FLFVSLV RP LFRKSMRKFSVAWWAYSFP+SVLALA  EYAKEV 
Sbjct: 246 GFDTFSKMLFFLSIFLFVSLVSRPGLFRKSMRKFSVAWWAYSFPLSVLALACNEYAKEV- 305

Query: 306 AGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGE 356
            GA AAHVFALLL+LLSVLVSLFLM VTV+RS+  IP  P E++SDSSS E
Sbjct: 306 -GAEAAHVFALLLALLSVLVSLFLMIVTVLRSHLFIPEIPTEITSDSSSTE 354

BLAST of Moc04g02580 vs. NCBI nr
Match: XP_022988831.1 (S-type anion channel SLAH1-like [Cucurbita maxima])

HSP 1 Score: 535.4 bits (1378), Expect = 3.7e-148
Identity = 282/353 (79.89%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 3   EMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPN 62
           EMD+NQ K  SFFL  LAKFHAGYFRISMSLC QALLWKILK PIQN+NSL +IL+ LPN
Sbjct: 2   EMDENQSKNPSFFLQFLAKFHAGYFRISMSLCGQALLWKILKQPIQNENSLRKILRLLPN 61

Query: 63  TAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSP 122
           +AFL LWSLALLIL+SLSL YILRCFFHFKLVK EFL+RVGVNYLFAPWISWLLLLQSSP
Sbjct: 62  SAFLVLWSLALLILVSLSLIYILRCFFHFKLVKFEFLHRVGVNYLFAPWISWLLLLQSSP 121

Query: 123 LKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAV 182
            K  P EILMWVF+IPI  LDVKIYGQWF KGKRFLSTVANPTSQLSVIGNLAGAR  AV
Sbjct: 122 FKTFPYEILMWVFLIPIVILDVKIYGQWFIKGKRFLSTVANPTSQLSVIGNLAGARTLAV 181

Query: 183 MGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSI 242
           MGWRET+LCMFSLGM+HYLVLFVTLYQRLAGSN LPA LRPVFFLFFA PSMAS+AW+SI
Sbjct: 182 MGWRETSLCMFSLGMSHYLVLFVTLYQRLAGSNSLPAILRPVFFLFFAVPSMASLAWSSI 241

Query: 243 NGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKE 302
             +FD FSK++FF+SVFLFVSL+ RP LFRK MRKF+VAWW+YSFP+S+LALA  EYAKE
Sbjct: 242 TKRFDTFSKILFFVSVFLFVSLISRPMLFRKCMRKFTVAWWSYSFPLSLLALACNEYAKE 301

Query: 303 VGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGE 356
           V  G  AAHV ALLL+LLS+ VSLFLM +TV+R+NS+IP S PE+SSDSS  E
Sbjct: 302 V--GVEAAHVCALLLALLSLFVSLFLMILTVLRTNSMIPMSSPEISSDSSGAE 352

BLAST of Moc04g02580 vs. ExPASy Swiss-Prot
Match: Q5E930 (S-type anion channel SLAH1 OS=Arabidopsis thaliana OX=3702 GN=SLAH1 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.2e-96
Identity = 190/329 (57.75%), Postives = 239/329 (72.64%), Query Frame = 0

Query: 19  LAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAFLFLWSLALLILLS 78
           L   HAGYFRIS+SLC+QALLWKI+  P     S++ +   LP+ AF  LW LAL+  +S
Sbjct: 38  LRSLHAGYFRISLSLCSQALLWKIMIAP--ESPSMSHMHSKLPSMAFHLLWYLALVTQVS 97

Query: 79  LSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPL---KFVPQEILMWVF 138
           L   Y L+C F F  VK EFL+ +GVNYL+AP ISWLL+LQS+P+     V  + L W+F
Sbjct: 98  LCFLYALKCIFFFDKVKEEFLHYIGVNYLYAPSISWLLMLQSAPMMEPNSVLYQTLFWIF 157

Query: 139 VIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMGWRETALCMFSL 198
            +P+  LD+K+YGQWFT  KRFLS +ANP SQ+SVI NL  AR AA MGW E ALCMFSL
Sbjct: 158 AVPVLTLDIKLYGQWFTTEKRFLSMLANPASQVSVIANLVAARGAAEMGWNECALCMFSL 217

Query: 199 GMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSINGKFDVFSKMMFF 258
           GM HYLV+FVTLYQRL G N  PA LRP+FFLF AAP+MAS+AWNSI G FD  +KM+FF
Sbjct: 218 GMVHYLVIFVTLYQRLPGGNNFPAKLRPIFFLFVAAPAMASLAWNSICGTFDAVAKMLFF 277

Query: 259 LSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVGAGAGAAHVFAL 318
           LS+F+F+SLV RP LF+KSM++F+VAWWAYSFP++ LAL S++YA+EV    G+     L
Sbjct: 278 LSLFIFMSLVCRPNLFKKSMKRFNVAWWAYSFPLTFLALDSVQYAQEVKDPVGSG--LML 337

Query: 319 LLSLLSVLVSLFLMAVTVMRSNSLIPASP 345
           + S +SVL+ L +M +T   SN L+   P
Sbjct: 338 IFSSISVLIFLGMMVLTAANSNRLLRHDP 362

BLAST of Moc04g02580 vs. ExPASy Swiss-Prot
Match: A8MRV9 (S-type anion channel SLAH4 OS=Arabidopsis thaliana OX=3702 GN=SLAH4 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.6e-91
Identity = 182/333 (54.65%), Postives = 233/333 (69.97%), Query Frame = 0

Query: 11  EASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAFLFLWS 70
           E    + +L+  HAGYFRIS+SLC+QALLWKI+ H          +   LP+ A+  LW 
Sbjct: 30  EPIVLMSVLSSLHAGYFRISLSLCSQALLWKIMVH----------LHSELPSMAYYLLWY 89

Query: 71  LALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPL---KFVP 130
           LAL   +SL   Y  +C F F +VK EF + +GVNYL+AP IS LLLLQS+P+     V 
Sbjct: 90  LALATQVSLCFLYAFKCIFLFDMVKEEFSHYIGVNYLYAPSISCLLLLQSAPMIEPHSVL 149

Query: 131 QEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMGWRE 190
            + L W+F +P+  LD K+YGQWFT  KRFLS +ANP SQ+SVI NL  AR AA MGW+E
Sbjct: 150 YQTLFWIFAVPVLTLDTKLYGQWFTTEKRFLSIMANPASQVSVIANLVAARGAAEMGWKE 209

Query: 191 TALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSINGKFD 250
            ALC+FSLGM HYLV+FVTLYQRL G N  P TLRPVFFLFFAAP+ AS+AWNSI G FD
Sbjct: 210 CALCLFSLGMVHYLVIFVTLYQRLPGGNNFPTTLRPVFFLFFAAPATASLAWNSICGNFD 269

Query: 251 VFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVGAGA 310
             +KM+FFLS+F+F+SLV RP L +KS+++F+VAWWAYSFP++ LAL S++YA+EV    
Sbjct: 270 TIAKMLFFLSLFIFISLVCRPNLLKKSIKRFNVAWWAYSFPITFLALNSVQYAQEV--KD 329

Query: 311 GAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLI 341
             A V   + S +SVL+ + +M +T   S  L+
Sbjct: 330 HVASVLMFIFSSMSVLIFISVMLLTAANSKRLL 350

BLAST of Moc04g02580 vs. ExPASy Swiss-Prot
Match: Q9LD83 (Guard cell S-type anion channel SLAC1 OS=Arabidopsis thaliana OX=3702 GN=SLAC1 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.8e-60
Identity = 147/347 (42.36%), Postives = 213/347 (61.38%), Query Frame = 0

Query: 1   MEEMDQNQ-----KKEASFFLLLLAKFHAGYFRISMSLCAQALLWKIL-KHPIQNQNSLT 60
           ++E+  N+     K+E   FLL   +F  G F I + L +QA+LW  L K P  N   +T
Sbjct: 165 LDEVKDNEDILLPKEEQWPFLL---RFPIGCFGICLGLSSQAVLWLALAKSPATNFLHIT 224

Query: 61  RILQFLPNTAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISW 120
            ++        L +W  +L++L+S+S TYIL+C F+F+ VK E+ + V VN+ FAPW+  
Sbjct: 225 PLIN-------LVVWLFSLVVLVSVSFTYILKCIFYFEAVKREYFHPVRVNFFFAPWVVC 284

Query: 121 LLLLQSSPLKFVPQE-----ILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLS 180
           + L  S P  F P        +  VF+ P   L++KIYGQW + GKR L  VANP+S LS
Sbjct: 285 MFLAISVPPMFSPNRKYLHPAIWCVFMGPYFFLELKIYGQWLSGGKRRLCKVANPSSHLS 344

Query: 181 VIGNLAGARAAAVMGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFF 240
           V+GN  GA  A+ +GW E A  ++++G AHYLV+FVTLYQRL  S  LP  L PV+ +F 
Sbjct: 345 VVGNFVGAILASKVGWDEVAKFLWAVGFAHYLVVFVTLYQRLPTSEALPKELHPVYSMFI 404

Query: 241 AAPSMASVAWNSINGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPV 300
           AAPS AS+AWN+I G+FD  S+  FF+++FL++SLV R   F  +  KFSVAWW+Y+FP+
Sbjct: 405 AAPSAASIAWNTIYGQFDGCSRTCFFIALFLYISLVARINFF--TGFKFSVAWWSYTFPM 464

Query: 301 SVLALASIEYAKEVGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRS 337
           +  ++A+I+YA+ V      A   AL LS +S  +   L   T++ +
Sbjct: 465 TTASVATIKYAEAVPGYPSRA--LALTLSFISTAMVCVLFVSTLLHA 497

BLAST of Moc04g02580 vs. ExPASy Swiss-Prot
Match: Q9ASQ7 (S-type anion channel SLAH2 OS=Arabidopsis thaliana OX=3702 GN=SLAH2 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.2e-55
Identity = 133/322 (41.30%), Postives = 205/322 (63.66%), Query Frame = 0

Query: 19  LAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAFL--FLWSLALLIL 78
           L +F    + + + + +QA++WK L        + T   +FL  T  +   LW ++LL+L
Sbjct: 140 LLRFPITSYGMCLGVSSQAIMWKTL--------ATTEAEKFLHVTQVINHVLWWISLLLL 199

Query: 79  LSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLKFVPQ-EILMWVF 138
           L++S+TY+ +    F+ V+ EF + + VN+ FAP IS L L    P   +      +W F
Sbjct: 200 LAVSITYLFKTILFFEAVRREFRHPIRVNFFFAPLISILFLALGIPHSIISHLPSTLWYF 259

Query: 139 VI-PIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMGWRETALCMFS 198
           ++ PI  L++KIYGQW + G+R LS VANPT+ LS++GN AGA   A MG +E  +  F+
Sbjct: 260 LMAPILFLEMKIYGQWMSGGQRRLSKVANPTNHLSIVGNFAGALLGASMGLKEGPIFFFA 319

Query: 199 LGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSINGKFDVFSKMMF 258
           +G+A+YLVLFVTLYQRL  +  LP  L PVFFLF AAP++AS+AW  I+  FD+ S++ +
Sbjct: 320 IGLAYYLVLFVTLYQRLPTNETLPKELHPVFFLFVAAPAVASMAWTKISASFDLGSRLAY 379

Query: 259 FLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVGAGAGAAHVFA 318
           F+S+FL+ SLV R  LFR    KFS+AWWAY+FP++ +A A+I+Y+ EV     A  + +
Sbjct: 380 FISLFLYFSLVCRINLFRGF--KFSLAWWAYTFPMTAVASATIKYSDEV--TGVATKILS 439

Query: 319 LLLSLLSVLVSLFLMAVTVMRS 337
           +++S  + L  + ++ +TVM +
Sbjct: 440 VVMSGAATLTVIAVLGLTVMHA 449

BLAST of Moc04g02580 vs. ExPASy Swiss-Prot
Match: Q9FLV9 (S-type anion channel SLAH3 OS=Arabidopsis thaliana OX=3702 GN=SLAH3 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 6.7e-52
Identity = 125/332 (37.65%), Postives = 198/332 (59.64%), Query Frame = 0

Query: 19  LAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAFLFLWSLALLILLS 78
           L ++    F + + + +QA++WK L          T+ L  +P      LW +++ ++L+
Sbjct: 255 LLRYPISTFGMCLGVSSQAIMWKTLA-----TAEPTKFLH-VPLWINQGLWFISVALILT 314

Query: 79  LSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLKFVPQ--EILMWVFV 138
           ++  Y+L+    F+ V+ E+ + + +N+ FAP+IS L L    P   +      L ++ +
Sbjct: 315 IATIYLLKIILFFEAVRREYYHPIRINFFFAPFISLLFLALGVPPSIITDLPHFLWYLLM 374

Query: 139 IPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMGWRETALCMFSLG 198
            P   L++KIYGQW + G+R LS VANPT+ LSV+GN  GA   A MG RE  +  +++G
Sbjct: 375 FPFICLELKIYGQWMSGGQRRLSRVANPTNHLSVVGNFVGALLGASMGLREGPIFFYAVG 434

Query: 199 MAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSINGKFDVFSKMMFFL 258
           MAHYLVLFVTLYQRL  +  LP  L PVFFLF AAPS+AS+AW  + G FD  SK+ +F+
Sbjct: 435 MAHYLVLFVTLYQRLPTNETLPKDLHPVFFLFVAAPSVASMAWAKVTGSFDYGSKVCYFI 494

Query: 259 SVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVGAGAGAAHVFALL 318
           ++FL+ SL +R   FR    KFS++WWAY+FP++  A+A+I YA  V   +    +  ++
Sbjct: 495 AIFLYFSLAVRINFFRGI--KFSLSWWAYTFPMTGAAIATIRYATVV--KSTMTQIMCVV 554

Query: 319 LSLLSVLVSLFLMAVTVMRSNSLIPASPPEVS 349
           L  ++ LV   L+  T++ +  L    P +++
Sbjct: 555 LCAIATLVVFALLVTTIIHAFVLRDLFPNDLA 576

BLAST of Moc04g02580 vs. ExPASy TrEMBL
Match: A0A6J1C6Y2 (S-type anion channel SLAH1-like OS=Momordica charantia OX=3673 GN=LOC111007939 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 9.4e-190
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 0

Query: 1   MEEMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFL 60
           MEEMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFL
Sbjct: 1   MEEMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFL 60

Query: 61  PNTAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQS 120
           PNTAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQS
Sbjct: 61  PNTAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQS 120

Query: 121 SPLKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAA 180
           SPLKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAA
Sbjct: 121 SPLKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAA 180

Query: 181 AVMGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWN 240
           AVMGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWN
Sbjct: 181 AVMGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWN 240

Query: 241 SINGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYA 300
           SINGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYA
Sbjct: 241 SINGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYA 300

Query: 301 KEVGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGEV 357
           KEVGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGEV
Sbjct: 301 KEVGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGEV 356

BLAST of Moc04g02580 vs. ExPASy TrEMBL
Match: A0A1S3C0X5 (S-type anion channel SLAH1-like OS=Cucumis melo OX=3656 GN=LOC103495607 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.1e-150
Identity = 288/351 (82.05%), Postives = 315/351 (89.74%), Query Frame = 0

Query: 6   QNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAF 65
           QN+ K+ SFFL LLAKFHAGYFRISMSLC QALLWKILK PIQN+NSL RIL+ LPNTAF
Sbjct: 6   QNEDKKPSFFLQLLAKFHAGYFRISMSLCGQALLWKILKQPIQNENSLRRILRLLPNTAF 65

Query: 66  LFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLK- 125
           L LWSL L I  SLSL YILRCFFHFKLVK+EFL+RVGVNYLFAPW+SWLLLLQSSP K 
Sbjct: 66  LLLWSLTLFISASLSLIYILRCFFHFKLVKSEFLHRVGVNYLFAPWLSWLLLLQSSPFKS 125

Query: 126 FVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMG 185
            +P +ILMWVF+IPI  LDVKIYGQWFTKGKRFLSTVANP+SQLSVIGNLAGARAAAV+G
Sbjct: 126 LIPNQILMWVFIIPIVVLDVKIYGQWFTKGKRFLSTVANPSSQLSVIGNLAGARAAAVIG 185

Query: 186 WRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSING 245
           WRE+ALC+FSLGMAHYLVLFVTLYQRL+GSN LPA LRPVFFLFFAAPSMAS+AW+SING
Sbjct: 186 WRESALCLFSLGMAHYLVLFVTLYQRLSGSNTLPAILRPVFFLFFAAPSMASLAWSSING 245

Query: 246 KFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVG 305
            FD FSKM+FFLSVFLFVSLV RP LFRKSMRKFS+AWWAYSFP+SVLALA  EYAKEV 
Sbjct: 246 GFDTFSKMLFFLSVFLFVSLVSRPGLFRKSMRKFSIAWWAYSFPLSVLALACNEYAKEV- 305

Query: 306 AGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGE 356
             A AAHVFALLL+L+SVLVSLFLM VTV+RS+ L+P +P E+SSDSSS E
Sbjct: 306 -EATAAHVFALLLALISVLVSLFLMIVTVLRSHLLVPQTPTEISSDSSSAE 354

BLAST of Moc04g02580 vs. ExPASy TrEMBL
Match: A0A0A0K5I9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G043050 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.6e-149
Identity = 288/351 (82.05%), Postives = 314/351 (89.46%), Query Frame = 0

Query: 6   QNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAF 65
           Q++ K+ SFFL LLAK HAGYFRISMSLC QALLWKILK PIQN+NSL RIL+ LPNTAF
Sbjct: 4   QDEVKKPSFFLQLLAKVHAGYFRISMSLCGQALLWKILKQPIQNENSLRRILRLLPNTAF 63

Query: 66  LFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLK- 125
           L LWSL L IL SLSL YILRCFFHFKLVK+EFL+RVGVNYLFAPW+SWLLLLQSSP K 
Sbjct: 64  LLLWSLTLFILASLSLIYILRCFFHFKLVKSEFLHRVGVNYLFAPWLSWLLLLQSSPFKS 123

Query: 126 FVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMG 185
            +P +ILMWVF+IPI  LDVKIYGQWFTKGKRFLSTVANP+SQLSVIGNLAGARAAAV+G
Sbjct: 124 LLPNQILMWVFIIPIVVLDVKIYGQWFTKGKRFLSTVANPSSQLSVIGNLAGARAAAVIG 183

Query: 186 WRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSING 245
           WRE+ALC+FSLGMAHYLVLFVTLYQRL+GSN LPA LRPVFFLFFAAPSMAS+AW+SING
Sbjct: 184 WRESALCLFSLGMAHYLVLFVTLYQRLSGSNTLPAILRPVFFLFFAAPSMASLAWSSING 243

Query: 246 KFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVG 305
            FD FSKM+FFLS+FLFVSLV RP LFRKSMRKFSVAWWAYSFP+SVLALA  EYAKEV 
Sbjct: 244 GFDTFSKMLFFLSIFLFVSLVSRPGLFRKSMRKFSVAWWAYSFPLSVLALACNEYAKEV- 303

Query: 306 AGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGE 356
            GA AAHVFALLL+LLSVLVSLFLM VTV+RS+  IP  P E++SDSSS E
Sbjct: 304 -GAEAAHVFALLLALLSVLVSLFLMIVTVLRSHLFIPEIPTEITSDSSSTE 352

BLAST of Moc04g02580 vs. ExPASy TrEMBL
Match: A0A6J1JIC9 (S-type anion channel SLAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111486061 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.8e-148
Identity = 282/353 (79.89%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 3   EMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPN 62
           EMD+NQ K  SFFL  LAKFHAGYFRISMSLC QALLWKILK PIQN+NSL +IL+ LPN
Sbjct: 2   EMDENQSKNPSFFLQFLAKFHAGYFRISMSLCGQALLWKILKQPIQNENSLRKILRLLPN 61

Query: 63  TAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSP 122
           +AFL LWSLALLIL+SLSL YILRCFFHFKLVK EFL+RVGVNYLFAPWISWLLLLQSSP
Sbjct: 62  SAFLVLWSLALLILVSLSLIYILRCFFHFKLVKFEFLHRVGVNYLFAPWISWLLLLQSSP 121

Query: 123 LKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAV 182
            K  P EILMWVF+IPI  LDVKIYGQWF KGKRFLSTVANPTSQLSVIGNLAGAR  AV
Sbjct: 122 FKTFPYEILMWVFLIPIVILDVKIYGQWFIKGKRFLSTVANPTSQLSVIGNLAGARTLAV 181

Query: 183 MGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSI 242
           MGWRET+LCMFSLGM+HYLVLFVTLYQRLAGSN LPA LRPVFFLFFA PSMAS+AW+SI
Sbjct: 182 MGWRETSLCMFSLGMSHYLVLFVTLYQRLAGSNSLPAILRPVFFLFFAVPSMASLAWSSI 241

Query: 243 NGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKE 302
             +FD FSK++FF+SVFLFVSL+ RP LFRK MRKF+VAWW+YSFP+S+LALA  EYAKE
Sbjct: 242 TKRFDTFSKILFFVSVFLFVSLISRPMLFRKCMRKFTVAWWSYSFPLSLLALACNEYAKE 301

Query: 303 VGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGE 356
           V  G  AAHV ALLL+LLS+ VSLFLM +TV+R+NS+IP S PE+SSDSS  E
Sbjct: 302 V--GVEAAHVCALLLALLSLFVSLFLMILTVLRTNSMIPMSSPEISSDSSGAE 352

BLAST of Moc04g02580 vs. ExPASy TrEMBL
Match: A0A6J1ESB3 (S-type anion channel SLAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111437120 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.2e-147
Identity = 281/353 (79.60%), Postives = 309/353 (87.54%), Query Frame = 0

Query: 3   EMDQNQKKEASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPN 62
           EMD+NQ K  SFFL  LAKFHAGYFRISMSLC QALLWKILK PIQN+NSL +IL+ LPN
Sbjct: 2   EMDENQTKNPSFFLQFLAKFHAGYFRISMSLCGQALLWKILKQPIQNENSLRKILRLLPN 61

Query: 63  TAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSP 122
           +AFL LWSL LLIL+SLSL YILRCFFHFKLVK EFL+RVGVNYLFAPWISWLLLLQSSP
Sbjct: 62  SAFLVLWSLTLLILVSLSLIYILRCFFHFKLVKFEFLHRVGVNYLFAPWISWLLLLQSSP 121

Query: 123 LKFVPQEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAV 182
            K  P EILMWVF+IPI  LDVKIYGQWF KGKRFLSTVANPTSQLSVIGNLAGAR  AV
Sbjct: 122 FKSFPYEILMWVFLIPIVILDVKIYGQWFIKGKRFLSTVANPTSQLSVIGNLAGARTLAV 181

Query: 183 MGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSI 242
           MGWRET+LCMFSLGM+HYLVLFVTLYQRLAGSN LPA LRPVFFLFFA PSMAS+AW+SI
Sbjct: 182 MGWRETSLCMFSLGMSHYLVLFVTLYQRLAGSNSLPAILRPVFFLFFAVPSMASLAWSSI 241

Query: 243 NGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKE 302
             +FD FSK++FF+SVFLFVSLV RP LFRK MRKF+VAWW+YSFP+S+LALA  EYAKE
Sbjct: 242 TKRFDTFSKILFFVSVFLFVSLVSRPMLFRKCMRKFTVAWWSYSFPLSLLALACNEYAKE 301

Query: 303 VGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLIPASPPEVSSDSSSGE 356
           V  G  AAHV ALLL+L+S+ VSLFLM +TV+R+NS+IP S PE+SSDSS  E
Sbjct: 302 V--GVEAAHVCALLLALVSLFVSLFLMILTVLRTNSMIPMSSPEISSDSSGAE 352

BLAST of Moc04g02580 vs. TAIR 10
Match: AT1G62280.1 (SLAC1 homologue 1 )

HSP 1 Score: 354.8 bits (909), Expect = 8.3e-98
Identity = 190/329 (57.75%), Postives = 239/329 (72.64%), Query Frame = 0

Query: 19  LAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAFLFLWSLALLILLS 78
           L   HAGYFRIS+SLC+QALLWKI+  P     S++ +   LP+ AF  LW LAL+  +S
Sbjct: 38  LRSLHAGYFRISLSLCSQALLWKIMIAP--ESPSMSHMHSKLPSMAFHLLWYLALVTQVS 97

Query: 79  LSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPL---KFVPQEILMWVF 138
           L   Y L+C F F  VK EFL+ +GVNYL+AP ISWLL+LQS+P+     V  + L W+F
Sbjct: 98  LCFLYALKCIFFFDKVKEEFLHYIGVNYLYAPSISWLLMLQSAPMMEPNSVLYQTLFWIF 157

Query: 139 VIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMGWRETALCMFSL 198
            +P+  LD+K+YGQWFT  KRFLS +ANP SQ+SVI NL  AR AA MGW E ALCMFSL
Sbjct: 158 AVPVLTLDIKLYGQWFTTEKRFLSMLANPASQVSVIANLVAARGAAEMGWNECALCMFSL 217

Query: 199 GMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSINGKFDVFSKMMFF 258
           GM HYLV+FVTLYQRL G N  PA LRP+FFLF AAP+MAS+AWNSI G FD  +KM+FF
Sbjct: 218 GMVHYLVIFVTLYQRLPGGNNFPAKLRPIFFLFVAAPAMASLAWNSICGTFDAVAKMLFF 277

Query: 259 LSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVGAGAGAAHVFAL 318
           LS+F+F+SLV RP LF+KSM++F+VAWWAYSFP++ LAL S++YA+EV    G+     L
Sbjct: 278 LSLFIFMSLVCRPNLFKKSMKRFNVAWWAYSFPLTFLALDSVQYAQEVKDPVGSG--LML 337

Query: 319 LLSLLSVLVSLFLMAVTVMRSNSLIPASP 345
           + S +SVL+ L +M +T   SN L+   P
Sbjct: 338 IFSSISVLIFLGMMVLTAANSNRLLRHDP 362

BLAST of Moc04g02580 vs. TAIR 10
Match: AT1G62262.1 (SLAC1 homologue 4 )

HSP 1 Score: 335.9 bits (860), Expect = 4.0e-92
Identity = 182/333 (54.65%), Postives = 233/333 (69.97%), Query Frame = 0

Query: 11  EASFFLLLLAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAFLFLWS 70
           E    + +L+  HAGYFRIS+SLC+QALLWKI+ H          +   LP+ A+  LW 
Sbjct: 30  EPIVLMSVLSSLHAGYFRISLSLCSQALLWKIMVH----------LHSELPSMAYYLLWY 89

Query: 71  LALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPL---KFVP 130
           LAL   +SL   Y  +C F F +VK EF + +GVNYL+AP IS LLLLQS+P+     V 
Sbjct: 90  LALATQVSLCFLYAFKCIFLFDMVKEEFSHYIGVNYLYAPSISCLLLLQSAPMIEPHSVL 149

Query: 131 QEILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMGWRE 190
            + L W+F +P+  LD K+YGQWFT  KRFLS +ANP SQ+SVI NL  AR AA MGW+E
Sbjct: 150 YQTLFWIFAVPVLTLDTKLYGQWFTTEKRFLSIMANPASQVSVIANLVAARGAAEMGWKE 209

Query: 191 TALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSINGKFD 250
            ALC+FSLGM HYLV+FVTLYQRL G N  P TLRPVFFLFFAAP+ AS+AWNSI G FD
Sbjct: 210 CALCLFSLGMVHYLVIFVTLYQRLPGGNNFPTTLRPVFFLFFAAPATASLAWNSICGNFD 269

Query: 251 VFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVGAGA 310
             +KM+FFLS+F+F+SLV RP L +KS+++F+VAWWAYSFP++ LAL S++YA+EV    
Sbjct: 270 TIAKMLFFLSLFIFISLVCRPNLLKKSIKRFNVAWWAYSFPITFLALNSVQYAQEV--KD 329

Query: 311 GAAHVFALLLSLLSVLVSLFLMAVTVMRSNSLI 341
             A V   + S +SVL+ + +M +T   S  L+
Sbjct: 330 HVASVLMFIFSSMSVLIFISVMLLTAANSKRLL 350

BLAST of Moc04g02580 vs. TAIR 10
Match: AT1G12480.1 (C4-dicarboxylate transporter/malic acid transport protein )

HSP 1 Score: 232.3 bits (591), Expect = 6.2e-61
Identity = 147/347 (42.36%), Postives = 213/347 (61.38%), Query Frame = 0

Query: 1   MEEMDQNQ-----KKEASFFLLLLAKFHAGYFRISMSLCAQALLWKIL-KHPIQNQNSLT 60
           ++E+  N+     K+E   FLL   +F  G F I + L +QA+LW  L K P  N   +T
Sbjct: 165 LDEVKDNEDILLPKEEQWPFLL---RFPIGCFGICLGLSSQAVLWLALAKSPATNFLHIT 224

Query: 61  RILQFLPNTAFLFLWSLALLILLSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISW 120
            ++        L +W  +L++L+S+S TYIL+C F+F+ VK E+ + V VN+ FAPW+  
Sbjct: 225 PLIN-------LVVWLFSLVVLVSVSFTYILKCIFYFEAVKREYFHPVRVNFFFAPWVVC 284

Query: 121 LLLLQSSPLKFVPQE-----ILMWVFVIPIAALDVKIYGQWFTKGKRFLSTVANPTSQLS 180
           + L  S P  F P        +  VF+ P   L++KIYGQW + GKR L  VANP+S LS
Sbjct: 285 MFLAISVPPMFSPNRKYLHPAIWCVFMGPYFFLELKIYGQWLSGGKRRLCKVANPSSHLS 344

Query: 181 VIGNLAGARAAAVMGWRETALCMFSLGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFF 240
           V+GN  GA  A+ +GW E A  ++++G AHYLV+FVTLYQRL  S  LP  L PV+ +F 
Sbjct: 345 VVGNFVGAILASKVGWDEVAKFLWAVGFAHYLVVFVTLYQRLPTSEALPKELHPVYSMFI 404

Query: 241 AAPSMASVAWNSINGKFDVFSKMMFFLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPV 300
           AAPS AS+AWN+I G+FD  S+  FF+++FL++SLV R   F  +  KFSVAWW+Y+FP+
Sbjct: 405 AAPSAASIAWNTIYGQFDGCSRTCFFIALFLYISLVARINFF--TGFKFSVAWWSYTFPM 464

Query: 301 SVLALASIEYAKEVGAGAGAAHVFALLLSLLSVLVSLFLMAVTVMRS 337
           +  ++A+I+YA+ V      A   AL LS +S  +   L   T++ +
Sbjct: 465 TTASVATIKYAEAVPGYPSRA--LALTLSFISTAMVCVLFVSTLLHA 497

BLAST of Moc04g02580 vs. TAIR 10
Match: AT4G27970.1 (SLAC1 homologue 2 )

HSP 1 Score: 217.6 bits (553), Expect = 1.6e-56
Identity = 133/322 (41.30%), Postives = 205/322 (63.66%), Query Frame = 0

Query: 19  LAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAFL--FLWSLALLIL 78
           L +F    + + + + +QA++WK L        + T   +FL  T  +   LW ++LL+L
Sbjct: 140 LLRFPITSYGMCLGVSSQAIMWKTL--------ATTEAEKFLHVTQVINHVLWWISLLLL 199

Query: 79  LSLSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLKFVPQ-EILMWVF 138
           L++S+TY+ +    F+ V+ EF + + VN+ FAP IS L L    P   +      +W F
Sbjct: 200 LAVSITYLFKTILFFEAVRREFRHPIRVNFFFAPLISILFLALGIPHSIISHLPSTLWYF 259

Query: 139 VI-PIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMGWRETALCMFS 198
           ++ PI  L++KIYGQW + G+R LS VANPT+ LS++GN AGA   A MG +E  +  F+
Sbjct: 260 LMAPILFLEMKIYGQWMSGGQRRLSKVANPTNHLSIVGNFAGALLGASMGLKEGPIFFFA 319

Query: 199 LGMAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSINGKFDVFSKMMF 258
           +G+A+YLVLFVTLYQRL  +  LP  L PVFFLF AAP++AS+AW  I+  FD+ S++ +
Sbjct: 320 IGLAYYLVLFVTLYQRLPTNETLPKELHPVFFLFVAAPAVASMAWTKISASFDLGSRLAY 379

Query: 259 FLSVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVGAGAGAAHVFA 318
           F+S+FL+ SLV R  LFR    KFS+AWWAY+FP++ +A A+I+Y+ EV     A  + +
Sbjct: 380 FISLFLYFSLVCRINLFRGF--KFSLAWWAYTFPMTAVASATIKYSDEV--TGVATKILS 439

Query: 319 LLLSLLSVLVSLFLMAVTVMRS 337
           +++S  + L  + ++ +TVM +
Sbjct: 440 VVMSGAATLTVIAVLGLTVMHA 449

BLAST of Moc04g02580 vs. TAIR 10
Match: AT5G24030.1 (SLAC1 homologue 3 )

HSP 1 Score: 206.1 bits (523), Expect = 4.8e-53
Identity = 125/332 (37.65%), Postives = 198/332 (59.64%), Query Frame = 0

Query: 19  LAKFHAGYFRISMSLCAQALLWKILKHPIQNQNSLTRILQFLPNTAFLFLWSLALLILLS 78
           L ++    F + + + +QA++WK L          T+ L  +P      LW +++ ++L+
Sbjct: 255 LLRYPISTFGMCLGVSSQAIMWKTLA-----TAEPTKFLH-VPLWINQGLWFISVALILT 314

Query: 79  LSLTYILRCFFHFKLVKAEFLNRVGVNYLFAPWISWLLLLQSSPLKFVPQ--EILMWVFV 138
           ++  Y+L+    F+ V+ E+ + + +N+ FAP+IS L L    P   +      L ++ +
Sbjct: 315 IATIYLLKIILFFEAVRREYYHPIRINFFFAPFISLLFLALGVPPSIITDLPHFLWYLLM 374

Query: 139 IPIAALDVKIYGQWFTKGKRFLSTVANPTSQLSVIGNLAGARAAAVMGWRETALCMFSLG 198
            P   L++KIYGQW + G+R LS VANPT+ LSV+GN  GA   A MG RE  +  +++G
Sbjct: 375 FPFICLELKIYGQWMSGGQRRLSRVANPTNHLSVVGNFVGALLGASMGLREGPIFFYAVG 434

Query: 199 MAHYLVLFVTLYQRLAGSNCLPATLRPVFFLFFAAPSMASVAWNSINGKFDVFSKMMFFL 258
           MAHYLVLFVTLYQRL  +  LP  L PVFFLF AAPS+AS+AW  + G FD  SK+ +F+
Sbjct: 435 MAHYLVLFVTLYQRLPTNETLPKDLHPVFFLFVAAPSVASMAWAKVTGSFDYGSKVCYFI 494

Query: 259 SVFLFVSLVLRPRLFRKSMRKFSVAWWAYSFPVSVLALASIEYAKEVGAGAGAAHVFALL 318
           ++FL+ SL +R   FR    KFS++WWAY+FP++  A+A+I YA  V   +    +  ++
Sbjct: 495 AIFLYFSLAVRINFFRGI--KFSLSWWAYTFPMTGAAIATIRYATVV--KSTMTQIMCVV 554

Query: 319 LSLLSVLVSLFLMAVTVMRSNSLIPASPPEVS 349
           L  ++ LV   L+  T++ +  L    P +++
Sbjct: 555 LCAIATLVVFALLVTTIIHAFVLRDLFPNDLA 576

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136183.11.9e-189100.00S-type anion channel SLAH1-like [Momordica charantia][more]
XP_038887173.11.1e-15284.05S-type anion channel SLAH1-like, partial [Benincasa hispida][more]
XP_008455447.12.3e-15082.05PREDICTED: S-type anion channel SLAH1-like [Cucumis melo][more]
XP_004144593.23.3e-14982.05S-type anion channel SLAH1 [Cucumis sativus] >KAE8645739.1 hypothetical protein ... [more]
XP_022988831.13.7e-14879.89S-type anion channel SLAH1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q5E9301.2e-9657.75S-type anion channel SLAH1 OS=Arabidopsis thaliana OX=3702 GN=SLAH1 PE=2 SV=1[more]
A8MRV95.6e-9154.65S-type anion channel SLAH4 OS=Arabidopsis thaliana OX=3702 GN=SLAH4 PE=2 SV=1[more]
Q9LD838.8e-6042.36Guard cell S-type anion channel SLAC1 OS=Arabidopsis thaliana OX=3702 GN=SLAC1 P... [more]
Q9ASQ72.2e-5541.30S-type anion channel SLAH2 OS=Arabidopsis thaliana OX=3702 GN=SLAH2 PE=2 SV=1[more]
Q9FLV96.7e-5237.65S-type anion channel SLAH3 OS=Arabidopsis thaliana OX=3702 GN=SLAH3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C6Y29.4e-190100.00S-type anion channel SLAH1-like OS=Momordica charantia OX=3673 GN=LOC111007939 P... [more]
A0A1S3C0X51.1e-15082.05S-type anion channel SLAH1-like OS=Cucumis melo OX=3656 GN=LOC103495607 PE=4 SV=... [more]
A0A0A0K5I91.6e-14982.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G043050 PE=4 SV=1[more]
A0A6J1JIC91.8e-14879.89S-type anion channel SLAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111486061 PE=3... [more]
A0A6J1ESB31.2e-14779.60S-type anion channel SLAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111437120 PE... [more]
Match NameE-valueIdentityDescription
AT1G62280.18.3e-9857.75SLAC1 homologue 1 [more]
AT1G62262.14.0e-9254.65SLAC1 homologue 4 [more]
AT1G12480.16.2e-6142.36C4-dicarboxylate transporter/malic acid transport protein [more]
AT4G27970.11.6e-5641.30SLAC1 homologue 2 [more]
AT5G24030.14.8e-5337.65SLAC1 homologue 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038665Voltage-dependent anion channel superfamilyGENE3D1.50.10.150coord: 17..339
e-value: 1.6E-60
score: 206.7
IPR004695Transporter protein SLAC1/Mae1/ Ssu1/TehAPFAMPF03595SLAC1coord: 21..333
e-value: 2.2E-45
score: 155.3
NoneNo IPR availablePANTHERPTHR31269:SF22OS11G0274300 PROTEINcoord: 14..349
NoneNo IPR availableCDDcd09323TDT_SLAC1_likecoord: 22..310
e-value: 7.34365E-74
score: 228.971
IPR030183S-type anion channelPANTHERPTHR31269FAMILY NOT NAMEDcoord: 14..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g02580.1Moc04g02580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006873 cellular ion homeostasis
biological_process GO:0015698 inorganic anion transport
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0008308 voltage-gated anion channel activity