Moc04g01510 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g01510
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionDiacylglycerol O-acyltransferase
Locationchr4: 985627 .. 988267 (-)
RNA-Seq ExpressionMoc04g01510
SyntenyMoc04g01510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATTCTTGAAGATTCAACAACTCCCATGAGCCCTTTGAGCCAATACTTCAACACCTCTGAAATGTGTGTTTCTGTTTTGGGAGTCCTCGAACTCGAATCTCCGATCTCTCCCCCTGACGCCATTTCAATTGTAACTGATGTTCTCCTCCCTGCCAACCCACGCTTTACTTCAATCATGGTACCTCCACTCTGCTCTAAAAATACGAATAAAAAGTTAAAATTACTCATTCAAACTACTGTAATGTCATTTTCTCTCCAATCAACAGGTAAAAGAGAAGTCAACGGGAGAAAGAAAGTGGAAGACAGCTGAAGTTAACCCAAGAGACCACATTTACGTACCGACTTTTCCAAACGACCTCTCGCCTTATGAATACGATGCTTACTTCGACGAATATGCCACGAAAACAGCCACTAAACCCTTCTCCCAAAGCATTCCTTTGTGGGAAATTCACGTTTTCAACTACCCTACAAGCCATGCAGCTTGTAGTATAATTTTCAAGGTTCATCACTCTATAGCCGATGGCTTCTGTCTTATGAACACTCTTTTATCTTGCCTTAAGAGAGCCGACGATCCTTCTCTTCCTCTCACGTTCCCGTCTCGAAAACACTCAAAACAACCCAAAAACGATGCTCGTTTTGGGCTCCTTAGCCATCTCCCTCGTCGAATCTCCTCCGTCGTATCAAACTTTGGATGGAGTGTGATGAAAAACACTTTTGTTGAAGACGACCCAACACCCATCAAGCCAGAAGGAGATTGCATGCAGCTTGTCAAGCCAATTGCCATATCCACCATGACCTTTTCCCTTGATCAAATCAAACAAATCAAGAACAACCTTAACGTGGTATATATATTACTTAATTATTTACTTATAAGGTCGTGATATATTTTGATATCTTTTTCTAATAATCAATATAGGATCAAGATTAAAAATATACCATCGAGATATATATATTTTGATTTGACAGTTTGATATGATCAACCAATACTTGTGTCATGTAAATATCAATAAAACATACAAAAAAAAAGTATCAATAAAAAGTTACCAATAGAAATACTAACAATTATAAGATGGTTAAATTTCAAATTTAATTGGGTACGTATGGGTTGAGCTTAATTTTAGTCTACATAGTCTTCAGAGTTTCAATTTGATTTCTATGCATGAATCATCATTAAATTATCTCTTATCCACTTGAAAAACCAAATAATTTTTTCTTTATTGAAAACATTAACTTATAGTCATATGAAAATAAAATAAAAAATGTCACTTATTTAATATGGTAACAATAAAGCCAAATCGAGTTTAGCTCAAGTGGTTAAGACATCTATCTTTGACTAAAAGGTCACAAATTCAAACCTCCCCCCACAATGTTGTACTAAAACAAATGGTAGCAATAAAAAGATAATTTTTAAGGTTTGATTAAATTATATGAACTGCAAATTGGTAGATTCATGACTTATTTTCATAAGATTGATGAAACTTGTTCTAGCTATAGATCTCCATTCACATCGATATTTTAACCCTGGTGTGGAATTTTGGTTTTTTAACAGAGTATTAATGATGTCCTCACCGGAATCATATTCATCGGAGTTCGACTATACATGCAAGAACACAACCCTCAGTCAAGTGAGAAAAATTCAAGTGCATTGATTCTCCTCAATACTAGGAAGGCTAAGGCTTACAAATCAGTTAAAGAGATGCTCGAAACCGACACCGATGCGCCGTGGGGAAACCGAATCGCCTTCTTGCCAGTCCCAATACCTAAACTAATTGATTCTACCCTTTCAAATAACCCTTTGGAATTTGTCAAGGAAGCCAAAGAAAAAATCATGTTGCAGAGAAACACTCTCTCCGTTTTCATGGCTGCCAAAGTTTTTGATATAGTAAACCACGTGACAGGCCCTGAGGTGAATATTTAACTTAACCCATTTCAAATTTCTTATAATATCTCCAGCTGATTAAACATGGTAATTCAATTTAATTATGATGATATCAATAATATAATGCTAATTAACTGTGTGCTAGGTTGGAGCAAAGTTGTTCAAAAGGAAGCTCAAGAATTCAAGCATCATGATATCAAGTATGATTGGGCCTTTGGAGAAAATGGCTTTGGCCAATCGTCCTGTTAAAGGCTTATACTTCACTGTCCCAGGCATGCCTCAGGTATCCAATCACAACTATTTTCTCACCAAGCTTAAGAGGGTGTAAGGGCAAGAATTTGTATTTCCACGTTTTCATCAAACAAACTCATGGGTTTCAACAGTATCCTAAACCTACTCCCTTCAAATCCTCTCAAGATCATGGGATCGGATACTCGTTTGAACTTTTGGGTTCTGATACCATATTAATATTCATCGAGTTCCTTCTAGAAGATGAGTAGTCCATGTCTATTATAAACTATTGTTAGGTCTCTTATTTTTTCAATGTGAGATACTTAACCGTGTGTGTATATATTTAACGTGTATCTGATTTATTTGTTTGTAGAGTCTACTTATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTATTTGGAGGTGAGAAGTGCTTCATAGATCAACAGAAATTGAAAGTATGTATTGAGGATGCCTTCAAAAGGACACTGATGAAAGCTGCTAAAAGAAGTTCGATCTAA

mRNA sequence

ATGGAGATTCTTGAAGATTCAACAACTCCCATGAGCCCTTTGAGCCAATACTTCAACACCTCTGAAATGTGTGTTTCTGTTTTGGGAGTCCTCGAACTCGAATCTCCGATCTCTCCCCCTGACGCCATTTCAATTGTAACTGATGTTCTCCTCCCTGCCAACCCACGCTTTACTTCAATCATGGTAAAAGAGAAGTCAACGGGAGAAAGAAAGTGGAAGACAGCTGAAGTTAACCCAAGAGACCACATTTACGTACCGACTTTTCCAAACGACCTCTCGCCTTATGAATACGATGCTTACTTCGACGAATATGCCACGAAAACAGCCACTAAACCCTTCTCCCAAAGCATTCCTTTGTGGGAAATTCACGTTTTCAACTACCCTACAAGCCATGCAGCTTGTAGTATAATTTTCAAGGTTCATCACTCTATAGCCGATGGCTTCTGTCTTATGAACACTCTTTTATCTTGCCTTAAGAGAGCCGACGATCCTTCTCTTCCTCTCACGTTCCCGTCTCGAAAACACTCAAAACAACCCAAAAACGATGCTCGTTTTGGGCTCCTTAGCCATCTCCCTCGTCGAATCTCCTCCGTCGTATCAAACTTTGGATGGAGTGTGATGAAAAACACTTTTGTTGAAGACGACCCAACACCCATCAAGCCAGAAGGAGATTGCATGCAGCTTGTCAAGCCAATTGCCATATCCACCATGACCTTTTCCCTTGATCAAATCAAACAAATCAAGAACAACCTTAACGTGAGTATTAATGATGTCCTCACCGGAATCATATTCATCGGAGTTCGACTATACATGCAAGAACACAACCCTCAGTCAAGTGAGAAAAATTCAAGTGCATTGATTCTCCTCAATACTAGGAAGGCTAAGGCTTACAAATCAGTTAAAGAGATGCTCGAAACCGACACCGATGCGCCGTGGGGAAACCGAATCGCCTTCTTGCCAGTCCCAATACCTAAACTAATTGATTCTACCCTTTCAAATAACCCTTTGGAATTTGTCAAGGAAGCCAAAGAAAAAATCATGTTGCAGAGAAACACTCTCTCCGTTTTCATGGCTGCCAAAGTTTTTGATATAGTAAACCACGTGACAGGCCCTGAGGTTGGAGCAAAGTTGTTCAAAAGGAAGCTCAAGAATTCAAGCATCATGATATCAAGTATGATTGGGCCTTTGGAGAAAATGGCTTTGGCCAATCGTCCTGTTAAAGGCTTATACTTCACTGTCCCAGGCATGCCTCAGAGTCTACTTATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTATTTGGAGGTGAGAAGTGCTTCATAGATCAACAGAAATTGAAAGTATGTATTGAGGATGCCTTCAAAAGGACACTGATGAAAGCTGCTAAAAGAAGTTCGATCTAA

Coding sequence (CDS)

ATGGAGATTCTTGAAGATTCAACAACTCCCATGAGCCCTTTGAGCCAATACTTCAACACCTCTGAAATGTGTGTTTCTGTTTTGGGAGTCCTCGAACTCGAATCTCCGATCTCTCCCCCTGACGCCATTTCAATTGTAACTGATGTTCTCCTCCCTGCCAACCCACGCTTTACTTCAATCATGGTAAAAGAGAAGTCAACGGGAGAAAGAAAGTGGAAGACAGCTGAAGTTAACCCAAGAGACCACATTTACGTACCGACTTTTCCAAACGACCTCTCGCCTTATGAATACGATGCTTACTTCGACGAATATGCCACGAAAACAGCCACTAAACCCTTCTCCCAAAGCATTCCTTTGTGGGAAATTCACGTTTTCAACTACCCTACAAGCCATGCAGCTTGTAGTATAATTTTCAAGGTTCATCACTCTATAGCCGATGGCTTCTGTCTTATGAACACTCTTTTATCTTGCCTTAAGAGAGCCGACGATCCTTCTCTTCCTCTCACGTTCCCGTCTCGAAAACACTCAAAACAACCCAAAAACGATGCTCGTTTTGGGCTCCTTAGCCATCTCCCTCGTCGAATCTCCTCCGTCGTATCAAACTTTGGATGGAGTGTGATGAAAAACACTTTTGTTGAAGACGACCCAACACCCATCAAGCCAGAAGGAGATTGCATGCAGCTTGTCAAGCCAATTGCCATATCCACCATGACCTTTTCCCTTGATCAAATCAAACAAATCAAGAACAACCTTAACGTGAGTATTAATGATGTCCTCACCGGAATCATATTCATCGGAGTTCGACTATACATGCAAGAACACAACCCTCAGTCAAGTGAGAAAAATTCAAGTGCATTGATTCTCCTCAATACTAGGAAGGCTAAGGCTTACAAATCAGTTAAAGAGATGCTCGAAACCGACACCGATGCGCCGTGGGGAAACCGAATCGCCTTCTTGCCAGTCCCAATACCTAAACTAATTGATTCTACCCTTTCAAATAACCCTTTGGAATTTGTCAAGGAAGCCAAAGAAAAAATCATGTTGCAGAGAAACACTCTCTCCGTTTTCATGGCTGCCAAAGTTTTTGATATAGTAAACCACGTGACAGGCCCTGAGGTTGGAGCAAAGTTGTTCAAAAGGAAGCTCAAGAATTCAAGCATCATGATATCAAGTATGATTGGGCCTTTGGAGAAAATGGCTTTGGCCAATCGTCCTGTTAAAGGCTTATACTTCACTGTCCCAGGCATGCCTCAGAGTCTACTTATAACAATTGTGAGTTACATGGGAGACTTGAGGATTGTATTTGGAGGTGAGAAGTGCTTCATAGATCAACAGAAATTGAAAGTATGTATTGAGGATGCCTTCAAAAGGACACTGATGAAAGCTGCTAAAAGAAGTTCGATCTAA

Protein sequence

MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPKNDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAKRSSI
Homology
BLAST of Moc04g01510 vs. NCBI nr
Match: XP_022147882.1 (O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransferase WSD1-like [Momordica charantia])

HSP 1 Score: 931.8 bits (2407), Expect = 2.3e-267
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60
           MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI
Sbjct: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60

Query: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120
           MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW
Sbjct: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120

Query: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180
           EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK
Sbjct: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180

Query: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS 240
           NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS
Sbjct: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS 240

Query: 241 LDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV 300
           LDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV
Sbjct: 241 LDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV 300

Query: 301 KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK 360
           KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK
Sbjct: 301 KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK 360

Query: 361 VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL 420
           VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL
Sbjct: 361 VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL 420

Query: 421 LITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAKRSSI 469
           LITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAKRSSI
Sbjct: 421 LITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAKRSSI 468

BLAST of Moc04g01510 vs. NCBI nr
Match: XP_011659709.2 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_017048 [Cucumis sativus])

HSP 1 Score: 764.6 bits (1973), Expect = 4.9e-217
Identity = 384/474 (81.01%), Postives = 425/474 (89.66%), Query Frame = 0

Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISP-PDAISIVTDVLLPANPRFTS 60
           MEI EDST PMSPLSQYFNT EMCVSVLGV+ELE+PIS   D  SIVTDVL+PANPRFTS
Sbjct: 1   MEIYEDSTAPMSPLSQYFNTCEMCVSVLGVIELETPISSWEDISSIVTDVLVPANPRFTS 60

Query: 61  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 120
           IMVKEK T ERKWKT +VNP+DHIY+P FP +LS YEYDAYFDEYATKTATKPFSQ+IPL
Sbjct: 61  IMVKEK-TEERKWKTTKVNPKDHIYIPNFPMELSSYEYDAYFDEYATKTATKPFSQAIPL 120

Query: 121 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 180
           WEIHVFNYPT+HA CSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 121 WEIHVFNYPTTHATCSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 180

Query: 181 KNDARFGLLSHLPRR----ISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAIS 240
            N+ +F  LSH P R    IS+ V NFGWS+MKNTFVEDDPTPIKP+ D MQLVKPIAIS
Sbjct: 181 GNEPKFFRLSHFPARFFSSISNFVLNFGWSIMKNTFVEDDPTPIKPQKDSMQLVKPIAIS 240

Query: 241 TMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 300
           TMTFSLDQIKQIKN LN S+NDVLTGIIF+G+RLYMQEHNP+SS  NSSALILLNTRKAK
Sbjct: 241 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHNPESSGANSSALILLNTRKAK 300

Query: 301 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 360
           AYKSVKEM++ D+DAPWGN+IAFLP+PIPKLIDS  +S+ PLEFV++ KEKIMLQR+ LS
Sbjct: 301 AYKSVKEMVKKDSDAPWGNKIAFLPIPIPKLIDSPVVSSTPLEFVEKVKEKIMLQRSPLS 360

Query: 361 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 420
           VF+AAKVF+I+ +VTGPE+GAKLFKRKLKNSSIMIS+MIGP+EKMAL N PVKGLYFTVP
Sbjct: 361 VFLAAKVFEILKNVTGPEIGAKLFKRKLKNSSIMISNMIGPVEKMALVNLPVKGLYFTVP 420

Query: 421 GMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAKRSSI 469
           GMPQSL+ITIVSYMGDLRIVFGGEKCFI+QQKLKVCIEDAF+R L KA  +  +
Sbjct: 421 GMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLKVCIEDAFQRILAKAVIKQKL 473

BLAST of Moc04g01510 vs. NCBI nr
Match: XP_022952997.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 733.4 bits (1892), Expect = 1.2e-207
Identity = 368/465 (79.14%), Postives = 405/465 (87.10%), Query Frame = 0

Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60
           MEILEDS  PMSPLSQYFNTS MCVSVLG +ELE+PIS   A++I T VL+PANPRF+SI
Sbjct: 1   MEILEDSAAPMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFSSI 60

Query: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120
           MVKEK  GERKWK  EVN  DHIY P FP DLSPYEYDAYFDEYAT TATKPFSQSIPLW
Sbjct: 61  MVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIPLW 120

Query: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180
           EIHVFNYPT+HA C++IFKVHHSIADGFCLMNTLLS LKRADDPSLPLTFPSRK SKQP+
Sbjct: 121 EIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQPE 180

Query: 181 NDARFGLLSHLPR----RISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAIST 240
           N   FG LSH P      + + +SNFG+S+  NTFVEDDPTPIK EGD MQLVKPIAIST
Sbjct: 181 NAHDFGRLSHFPACLFLSLRNFLSNFGYSI-ANTFVEDDPTPIKHEGDSMQLVKPIAIST 240

Query: 241 MTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKA 300
           MTFSLDQIKQIK  LN  +NDV+TGIIF+G+RLYMQEH+P S E NSSALILLNTRKAKA
Sbjct: 241 MTFSLDQIKQIKTKLNARVNDVITGIIFLGIRLYMQEHSPDSWEANSSALILLNTRKAKA 300

Query: 301 YKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVF 360
           YKSV+EM++TD+D PWGNRIAFLPVPIPKL+DSTLS  PLEFVKEAK+ IMLQR+ LSVF
Sbjct: 301 YKSVQEMVKTDSDVPWGNRIAFLPVPIPKLMDSTLSTAPLEFVKEAKKNIMLQRSPLSVF 360

Query: 361 MAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGM 420
           MAAKVFD++ HVTGPE+GAKLFKRKL NSS +IS+MIGP EKMAL NRPVKGLYFTVPG+
Sbjct: 361 MAAKVFDMLKHVTGPEMGAKLFKRKLNNSSTIISNMIGPTEKMALVNRPVKGLYFTVPGV 420

Query: 421 PQSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMK 462
           PQSL+ITIVSYMGDLRIVFGGEKCFID  KLK C+EDAF+R L+K
Sbjct: 421 PQSLIITIVSYMGDLRIVFGGEKCFIDLHKLKACVEDAFQRMLIK 464

BLAST of Moc04g01510 vs. NCBI nr
Match: XP_038887587.1 (O-acyltransferase WSD1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 676.0 bits (1743), Expect = 2.3e-190
Identity = 333/425 (78.35%), Postives = 380/425 (89.41%), Query Frame = 0

Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAI-SIVTDVLLPANPRFTS 60
           ME  E+ST PMSP+SQYFNTSEMCVSVLGV+ELE+PIS  + I SI  DVL+P+NPRFTS
Sbjct: 1   MENYEESTAPMSPMSQYFNTSEMCVSVLGVIELETPISAWEEISSIAIDVLIPSNPRFTS 60

Query: 61  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 120
           +MVKE S GERKWKT ++NP+DHIY+P FP +LSPYEYDAYFDEYAT+TATKPFSQSIPL
Sbjct: 61  VMVKESSNGERKWKTIKINPKDHIYIPNFPPELSPYEYDAYFDEYATQTATKPFSQSIPL 120

Query: 121 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 180
           WEIHVFNYPTSHA CSIIFKVHHSIADGFCLMN LLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 121 WEIHVFNYPTSHATCSIIFKVHHSIADGFCLMNILLSCLKRADDPSLPLTFPSRQRSKQP 180

Query: 181 KNDARFGLLSHLPRRISSVVS----NFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAIS 240
           +N+  F   S+ P  + S VS    NFGWS+MKNT VEDDPTPIKPE D MQLVKPIAIS
Sbjct: 181 ENELNFRRRSYFPGHLFSSVSNFLLNFGWSIMKNTIVEDDPTPIKPERDSMQLVKPIAIS 240

Query: 241 TMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 300
           T+TF LDQIKQIK+ LN S+NDV+TGIIF+G+RLYMQEHNP+SS+ NSSALILLNTRKAK
Sbjct: 241 TITFPLDQIKQIKDKLNASVNDVVTGIIFLGIRLYMQEHNPESSQANSSALILLNTRKAK 300

Query: 301 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 360
           AYKSVKEM++T+TDAPWGN+IAFLPVPIPKLIDS TL++ PLEFVK+AKEKIMLQR+ +S
Sbjct: 301 AYKSVKEMVKTNTDAPWGNKIAFLPVPIPKLIDSTTLASTPLEFVKKAKEKIMLQRSPVS 360

Query: 361 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 420
           VF+AAK F++V +VTGPEVGAKLFKRKLKNSSIMIS+MIGP+EK++L N PVKG+YFTVP
Sbjct: 361 VFLAAKFFEMVKNVTGPEVGAKLFKRKLKNSSIMISNMIGPVEKISLVNHPVKGIYFTVP 420

BLAST of Moc04g01510 vs. NCBI nr
Match: XP_038887586.1 (O-acyltransferase WSD1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 675.2 bits (1741), Expect = 3.9e-190
Identity = 333/424 (78.54%), Postives = 379/424 (89.39%), Query Frame = 0

Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAI-SIVTDVLLPANPRFTS 60
           ME  E+ST PMSP+SQYFNTSEMCVSVLGV+ELE+PIS  + I SI  DVL+P+NPRFTS
Sbjct: 1   MENYEESTAPMSPMSQYFNTSEMCVSVLGVIELETPISAWEEISSIAIDVLIPSNPRFTS 60

Query: 61  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 120
           +MVKE S GERKWKT ++NP+DHIY+P FP +LSPYEYDAYFDEYAT+TATKPFSQSIPL
Sbjct: 61  VMVKESSNGERKWKTIKINPKDHIYIPNFPPELSPYEYDAYFDEYATQTATKPFSQSIPL 120

Query: 121 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 180
           WEIHVFNYPTSHA CSIIFKVHHSIADGFCLMN LLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 121 WEIHVFNYPTSHATCSIIFKVHHSIADGFCLMNILLSCLKRADDPSLPLTFPSRQRSKQP 180

Query: 181 KNDARFGLLSHLPRRISSVVS----NFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAIS 240
           +N+  F   S+ P  + S VS    NFGWS+MKNT VEDDPTPIKPE D MQLVKPIAIS
Sbjct: 181 ENELNFRRRSYFPGHLFSSVSNFLLNFGWSIMKNTIVEDDPTPIKPERDSMQLVKPIAIS 240

Query: 241 TMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 300
           T+TF LDQIKQIK+ LN S+NDV+TGIIF+G+RLYMQEHNP+SS+ NSSALILLNTRKAK
Sbjct: 241 TITFPLDQIKQIKDKLNASVNDVVTGIIFLGIRLYMQEHNPESSQANSSALILLNTRKAK 300

Query: 301 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 360
           AYKSVKEM++T+TDAPWGN+IAFLPVPIPKLIDS TL++ PLEFVK+AKEKIMLQR+ +S
Sbjct: 301 AYKSVKEMVKTNTDAPWGNKIAFLPVPIPKLIDSTTLASTPLEFVKKAKEKIMLQRSPVS 360

Query: 361 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 419
           VF+AAK F++V +VTGPEVGAKLFKRKLKNSSIMIS+MIGP+EK++L N PVKG+YFTVP
Sbjct: 361 VFLAAKFFEMVKNVTGPEVGAKLFKRKLKNSSIMISNMIGPVEKISLVNHPVKGIYFTVP 420

BLAST of Moc04g01510 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 6.4e-42
Identity = 132/501 (26.35%), Postives = 235/501 (46.91%), Query Frame = 0

Query: 4   LEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVK 63
           +E+   P+SP ++ F+  E    ++ V+ L++ I P   I  +   L+  +PRF+S +V 
Sbjct: 21  VEEEEQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVN 80

Query: 64  --EKSTGERKWKTAEVNPRDHIYVPTFPND-LSPYEYDAYFDEYATKTATKPFSQSIPLW 123
               +  E+KW    V   DH+ +P      +     D + + Y +   T P   S PLW
Sbjct: 81  NCNNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLW 140

Query: 124 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 183
           E+H+ +  TS A    + ++HHS+ DG  +M+ +L+C ++  +P+     PS  +  +P 
Sbjct: 141 EVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPN---ELPSLPYQNRPS 200

Query: 184 NDARFGLLSHLPRRISSVVSNFGWSVM---------KNT------------FVEDDPTPI 243
           +       S   +  S   S F W VM          NT            F++D  TPI
Sbjct: 201 SG------SSSLKTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPI 260

Query: 244 KPEGDC-MQLVKPIAISTMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEH--NP 303
           K  GD  +   K + +   T SLD IK IKN + +++NDV+ G+   G+  Y++      
Sbjct: 261 K--GDFKLSKGKRMCMVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQ 320

Query: 304 QSSEKNSS---------ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLI 363
           + S++NSS         A +L+N R     + + +M+   +   WGN I ++  P     
Sbjct: 321 EESKRNSSNIPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFP----F 380

Query: 364 DSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSI 423
              L ++PL+ ++ AK  I  ++N+L   +   V  I+ +  G +  A +  R L N+++
Sbjct: 381 SIALCDDPLKHLRRAKSTIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTM 440

Query: 424 MISSMIGPLEKMALANRPVKGLYFTVPGMPQSLLITIVSYMGDLRIVFGGEKCFI-DQQK 468
             S+++GP+E+++     V  +  +V G P +L +   SYM  L I    +   I D  K
Sbjct: 441 SFSNLVGPVEEISFYGHTVTYIAPSVYGHPHALTMHFQSYMNKLTISLTVDPTVISDPHK 500

BLAST of Moc04g01510 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 9.2e-41
Identity = 123/491 (25.05%), Postives = 234/491 (47.66%), Query Frame = 0

Query: 5   EDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKE 64
           ++   P+SP ++ F+  E   +++ V+  +S + P   I    +  +  +PRF+S +V +
Sbjct: 14  KEEEQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFI-RHPRFSSKLVTD 73

Query: 65  KSTGERKWKTAEVNPRDHIYVPTF-PNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIH 124
           ++   ++W    V   DH  VP   P ++     +A+ ++Y +     P   S PLWE+H
Sbjct: 74  ENGQNQRWVRTNVVVEDHFIVPKIKPQNIE--NSNAFLEDYVSDLMKIPLDTSRPLWELH 133

Query: 125 VFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADD----PSLPLTFPSRKHSK-- 184
           + +  TS A    + K+HHS+ DG  +M+ +L+C+++  +    PSLP  + S   S   
Sbjct: 134 LLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLL 193

Query: 185 --QPKNDARFGLL-----SHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL-V 244
               ++D+R   L     + +   +++V     + ++   FV+D  TPIK  GD +    
Sbjct: 194 TTGSRSDSRLLWLVKVIWTAVILGLNTVCDALEF-IVTTLFVKDTETPIK--GDFLSTKS 253

Query: 245 KPIAISTMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSS----- 304
           K + +   T SLD IK  KN +N++INDV+ G+   G+  Y+     +   KN       
Sbjct: 254 KQLRLVHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLP 313

Query: 305 ------ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEF 364
                 + +L+N R     + + +M+E  +   WGN   ++  P        L ++PLE 
Sbjct: 314 KRIRLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFP----FSIALRDDPLEH 373

Query: 365 VKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEK 424
           ++ A++ I  ++N+    +      I+  + G +V A +  R + N+++  S+M+GP+E+
Sbjct: 374 LEIAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEE 433

Query: 425 MALANRPVKGLYFTVPGMPQSLLITIVSYMGDLRIVFGGEKCFI-DQQKLKVCIEDAFKR 469
           ++    P+     +  G P +L I   SYM  + I    +   I D  +L    E++ + 
Sbjct: 434 VSFYGHPITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPHRLCDDWEESLRS 493

BLAST of Moc04g01510 vs. ExPASy Swiss-Prot
Match: Q94CK0 (Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=WSD7 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.1e-38
Identity = 125/481 (25.99%), Postives = 223/481 (46.36%), Query Frame = 0

Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVL---LPANPRFTSIMVKEKS 69
           P+SP+++ F +  + +  + ++  ++ I+P     +V D L   +  +PRF+SI+    S
Sbjct: 8   PVSPMARVFQSPGIDLCAVTIMGFKTKINP----DVVLDALKQNVSKHPRFSSIL----S 67

Query: 70  TGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIHVFN 129
               KW   EVN  DH+ VP    +       ++ D+Y ++    P  +S PLW+IH+ N
Sbjct: 68  DNGAKWIETEVNVEDHVIVPYIDAEEIGEGGQSFIDDYMSRLTMIPLDRSRPLWDIHILN 127

Query: 130 YPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSL-PLTFPSRK------HSKQP 189
             TS A      + HHS+ DG  L++ +L+C  +  DP +     PS K      HS + 
Sbjct: 128 VKTSEAEAVGFIRSHHSLGDGMSLISLMLACTHKTSDPDMFSNAIPSMKRRATMSHSLKT 187

Query: 190 KNDARFGL--LSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTM 249
           K      +  +    R + +   +    +    F++D  TP+K   D      P      
Sbjct: 188 KGWFLRSIFTIGSTMRLLWNTTIDMLLLLATVLFLKDTKTPLKAGADVRS--NPKRFYHR 247

Query: 250 TFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYM--------QEHNPQSSEKNS------ 309
             SLD IK IKN +N++INDVL GI    +  Y+        +E    +S +N+      
Sbjct: 248 IISLDDIKLIKNAMNMTINDVLFGITQASLSHYLNRQYGTKKEEDGALTSYRNNLPDGIR 307

Query: 310 -SALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEA 369
                 +N R    +K + +M+  D+   WGN  +F+ +P        L  +PL ++K +
Sbjct: 308 FRVACTVNLRSDIGFKPLADMMVKDSKCRWGNYFSFIFLP----FTIGLQTDPLVYLKMS 367

Query: 370 KEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALA 429
           K  +  ++++    +   +  IV  V G +  A+LF R ++N++  +S++IGP+E+++  
Sbjct: 368 KSMMARKKHSYHAALVYFIIKIVLKVFGAKAAAELFDRPVRNTTTCVSNVIGPMEEISFR 427

Query: 430 NRPVKGLYFTVPGMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKA 464
             PV  +  +  G   +LLI ++SY   + I    +   I     K+C +       MKA
Sbjct: 428 GHPVSYIAPSSYGHSHALLIHLMSYADKMIISLAYDPTVISDPH-KICDDMEESLKAMKA 473

BLAST of Moc04g01510 vs. ExPASy Swiss-Prot
Match: F4IU14 (Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=WSD3 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-38
Identity = 119/437 (27.23%), Postives = 215/437 (49.20%), Query Frame = 0

Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP+SQ F +  +   ++  L  ++  +P   +  V +  +   PRF+S  V+ K  G+
Sbjct: 22  PLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKL-PRFSS-KVEIKKNGK 81

Query: 70  RKWKTAEVNPRDHIYVPTFPNDLSPYEY-DAYFDEYATKTATKPFSQSIPLWEIHVFNYP 129
             W    V   DH+ VP    D S  E  D + ++Y +K A  P   S PLWE+H+ N  
Sbjct: 82  ASWVPVSVRVEDHVVVPDL--DYSNIENPDQFIEDYTSKLANTPMDMSRPLWELHLLNIK 141

Query: 130 TSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDP-SLPLTFPSRKH-SKQPKNDARFG 189
           TS+A    I K HHS+ DG  L++ LL+  ++  DP +LP T  +RKH S   K+    G
Sbjct: 142 TSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKKSWWLVG 201

Query: 190 LLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPE-GDCMQLVKPIAISTMTFSLDQIK 249
               + R I + V      ++   F+ D  TP+  + GD ++  K I       S D +K
Sbjct: 202 RFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLMGKTGDAIRSRKVI---HRIVSFDDVK 261

Query: 250 QIKNNLNVSINDVLTGIIFIGVRLYM----QEHNPQSSEKNSSAL-----ILLNTRKAKA 309
            +KNN+++ +NDVL G+   G+  Y+     E      +KN   +     + +N R    
Sbjct: 262 LVKNNMDMKVNDVLLGMTQAGLSRYLSRKYDEDMVVEKKKNLEKIRLRGTVFVNLRADTK 321

Query: 310 YKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVF 369
            + +  M+   +   WGN +  +  P+   + S   ++PLE+V+ AK  + +++ ++   
Sbjct: 322 LEDLANMMAKGSKCRWGNFVGVIIFPL--WVRS--EDDPLEYVRRAKSTMDIKKLSIESL 381

Query: 370 MAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGM 429
           +   +  +   + G +V   L +R   ++++  S+++GP E ++  + P+  +  +  G 
Sbjct: 382 ICYGLIKLTRKILGGKVVETLVRRLFDHTTLTFSNVMGPDEDISFFDHPMSYVAASALGG 441

Query: 430 PQSLLITIVSYMGDLRI 434
           PQ+L+I  V+Y+  + I
Sbjct: 442 PQALIIHYVTYVNKIVI 447

BLAST of Moc04g01510 vs. ExPASy Swiss-Prot
Match: Q9M3B1 (Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=WSD6 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-38
Identity = 121/461 (26.25%), Postives = 214/461 (46.42%), Query Frame = 0

Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP ++ F+  E    V+ V+ ++  I P   I  +   L+  +PRF+S MV   S G 
Sbjct: 27  PLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQTLI-RHPRFSSKMV-STSVGN 86

Query: 70  RK-----WKTAEVNPRDHIYVPTF-PNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIH 129
           +K     W    V   DH+ V      ++     DA+ + Y +   T P   S PLW++H
Sbjct: 87  KKRQTQSWVRTNVVVTDHVIVSDIQTQNIENGNADAFLETYVSNLTTVPLDISKPLWQLH 146

Query: 130 VFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQ----- 189
           + +  TS A    + K HHS+ DG  LM  +L+C+++  +P    + P++  S       
Sbjct: 147 LLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLPNQNRSSSRSSRL 206

Query: 190 ---PKNDARFGLLSHLPRRISSVVSNFGWSVMK----NTFVEDDPTPIKPEGDC-MQLVK 249
               + D+RF  L  +      +V N     ++      F++D  TPIK  GD      K
Sbjct: 207 MAGSRGDSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPIK--GDFRFSKSK 266

Query: 250 PIAISTMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEH---------NPQSSEK 309
            + +   T SLD IK IKN + +++NDV+ G+   G+  Y+              Q S++
Sbjct: 267 RMCLVHRTVSLDDIKLIKNTMKMTVNDVVLGVSQAGLSQYLDRRYGEKKKKVGEDQDSKR 326

Query: 310 NSS---------ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLS 369
            ++         + +L+N R     + + +M+   +   WGN I ++  P        L 
Sbjct: 327 KATDMPKRIRLRSALLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIVFP----FSIGLR 386

Query: 370 NNPLEFVKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSM 429
           ++PL+ ++ AK  I  ++N+L   +       +    G +V AK+  R L N+++  S++
Sbjct: 387 DDPLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQVAAKIINRALSNTTMSFSNL 446

Query: 430 IGPLEKMALANRPVKGLYFTVPGMPQSLLITIVSYMGDLRI 434
           IGP+E+++    P+  +  +V G P +L +   SYM  + I
Sbjct: 447 IGPIEEISFYGHPITYMAPSVYGHPHALTMHFQSYMNQMTI 479

BLAST of Moc04g01510 vs. ExPASy TrEMBL
Match: A0A6J1D2I7 (Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 PE=4 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.1e-267
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60
           MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI
Sbjct: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60

Query: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120
           MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW
Sbjct: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120

Query: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180
           EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK
Sbjct: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180

Query: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS 240
           NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS
Sbjct: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS 240

Query: 241 LDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV 300
           LDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV
Sbjct: 241 LDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKAYKSV 300

Query: 301 KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK 360
           KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK
Sbjct: 301 KEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAK 360

Query: 361 VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL 420
           VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL
Sbjct: 361 VFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGMPQSL 420

Query: 421 LITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAKRSSI 469
           LITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAKRSSI
Sbjct: 421 LITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAKRSSI 468

BLAST of Moc04g01510 vs. ExPASy TrEMBL
Match: A0A6J1GLZ3 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 PE=4 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 5.9e-208
Identity = 368/465 (79.14%), Postives = 405/465 (87.10%), Query Frame = 0

Query: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60
           MEILEDS  PMSPLSQYFNTS MCVSVLG +ELE+PIS   A++I T VL+PANPRF+SI
Sbjct: 1   MEILEDSAAPMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFSSI 60

Query: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120
           MVKEK  GERKWK  EVN  DHIY P FP DLSPYEYDAYFDEYAT TATKPFSQSIPLW
Sbjct: 61  MVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIPLW 120

Query: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180
           EIHVFNYPT+HA C++IFKVHHSIADGFCLMNTLLS LKRADDPSLPLTFPSRK SKQP+
Sbjct: 121 EIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQPE 180

Query: 181 NDARFGLLSHLPR----RISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAIST 240
           N   FG LSH P      + + +SNFG+S+  NTFVEDDPTPIK EGD MQLVKPIAIST
Sbjct: 181 NAHDFGRLSHFPACLFLSLRNFLSNFGYSI-ANTFVEDDPTPIKHEGDSMQLVKPIAIST 240

Query: 241 MTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAKA 300
           MTFSLDQIKQIK  LN  +NDV+TGIIF+G+RLYMQEH+P S E NSSALILLNTRKAKA
Sbjct: 241 MTFSLDQIKQIKTKLNARVNDVITGIIFLGIRLYMQEHSPDSWEANSSALILLNTRKAKA 300

Query: 301 YKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVF 360
           YKSV+EM++TD+D PWGNRIAFLPVPIPKL+DSTLS  PLEFVKEAK+ IMLQR+ LSVF
Sbjct: 301 YKSVQEMVKTDSDVPWGNRIAFLPVPIPKLMDSTLSTAPLEFVKEAKKNIMLQRSPLSVF 360

Query: 361 MAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGM 420
           MAAKVFD++ HVTGPE+GAKLFKRKL NSS +IS+MIGP EKMAL NRPVKGLYFTVPG+
Sbjct: 361 MAAKVFDMLKHVTGPEMGAKLFKRKLNNSSTIISNMIGPTEKMALVNRPVKGLYFTVPGV 420

Query: 421 PQSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMK 462
           PQSL+ITIVSYMGDLRIVFGGEKCFID  KLK C+EDAF+R L+K
Sbjct: 421 PQSLIITIVSYMGDLRIVFGGEKCFIDLHKLKACVEDAFQRMLIK 464

BLAST of Moc04g01510 vs. ExPASy TrEMBL
Match: A0A0A0K2E9 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051330 PE=4 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 5.5e-190
Identity = 333/414 (80.43%), Postives = 372/414 (89.86%), Query Frame = 0

Query: 60  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 119
           + VKEK T ERKWKT +VNP+DHIY+P FP +LS YEYDAYFDEYATKTATKPFSQ+IPL
Sbjct: 1   MQVKEK-TEERKWKTTKVNPKDHIYIPNFPMELSSYEYDAYFDEYATKTATKPFSQAIPL 60

Query: 120 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 179
           WEIHVFNYPT+HA CSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 61  WEIHVFNYPTTHATCSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 180 KNDARFGLLSHLPRR----ISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAIS 239
            N+ +F  LSH P R    IS+ V NFGWS+MKNTFVEDDPTPIKP+ D MQLVKPIAIS
Sbjct: 121 GNEPKFFRLSHFPARFFSSISNFVLNFGWSIMKNTFVEDDPTPIKPQKDSMQLVKPIAIS 180

Query: 240 TMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 299
           TMTFSLDQIKQIKN LN S+NDVLTGIIF+G+RLYMQEHNP+SS  NSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGIRLYMQEHNPESSGANSSALILLNTRKAK 240

Query: 300 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 359
           AYKSVKEM++ D+DAPWGN+IAFLP+PIPKLIDS  +S+ PLEFV++ KEKIMLQR+ LS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPIPIPKLIDSPVVSSTPLEFVEKVKEKIMLQRSPLS 300

Query: 360 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 419
           VF+AAKVF+I+ +VTGPE+GAKLFKRKLKNSSIMIS+MIGP+EKMAL N PVKGLYFTVP
Sbjct: 301 VFLAAKVFEILKNVTGPEIGAKLFKRKLKNSSIMISNMIGPVEKMALVNLPVKGLYFTVP 360

Query: 420 GMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKAAKRSSI 469
           GMPQSL+ITIVSYMGDLRIVFGGEKCFI+QQKLKVCIEDAF+R L KA  +  +
Sbjct: 361 GMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLKVCIEDAFQRILAKAVIKQKL 413

BLAST of Moc04g01510 vs. ExPASy TrEMBL
Match: A0A5D3C6U2 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002990 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 7.5e-187
Identity = 328/403 (81.39%), Postives = 365/403 (90.57%), Query Frame = 0

Query: 60  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 119
           + VKEK T ERKWKT +V+ +DHI +P FP +LS YEYDAYFDEYATKTATKPFSQ+IPL
Sbjct: 1   MQVKEK-TEERKWKTTKVSLKDHICIPNFPTELSSYEYDAYFDEYATKTATKPFSQAIPL 60

Query: 120 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 179
           WEIHVFNYPT+HA CS+IFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 61  WEIHVFNYPTTHATCSVIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 180 KNDARFGLLSHLPRR----ISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAIS 239
           KN   F  LSH P R    +S+ V NFGWS+MKNTFVEDDPTPIKP+ D MQLVKPIAIS
Sbjct: 121 KNKLDFCRLSHFPARFFSSVSNFVLNFGWSIMKNTFVEDDPTPIKPQEDSMQLVKPIAIS 180

Query: 240 TMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 299
           TMTFSLDQIKQIKN LN S+NDVLTGIIF+GVRLYMQEHNP+SSE NSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGVRLYMQEHNPESSEANSSALILLNTRKAK 240

Query: 300 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 359
           AYKSVKEM++ D+DAPWGN+IAFLPVPIPKLIDS  +S+ PLEFV++ KEKI LQR+ LS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPVPIPKLIDSPVVSSTPLEFVEKVKEKITLQRSPLS 300

Query: 360 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 419
           VF+AAKVF+I+ +VTGPE+GAKLFKRKLKNSSIMIS++IGP+EKMAL NRPVKGLYFTVP
Sbjct: 301 VFLAAKVFEILKNVTGPEIGAKLFKRKLKNSSIMISNLIGPVEKMALVNRPVKGLYFTVP 360

Query: 420 GMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKR 458
           GMPQSL+ITIVSYMGDLRIVFGGEKCFI+QQKLKVCIEDAF+R
Sbjct: 361 GMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLKVCIEDAFQR 402

BLAST of Moc04g01510 vs. ExPASy TrEMBL
Match: A0A5A7SPR9 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G002950 PE=4 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.7e-186
Identity = 327/403 (81.14%), Postives = 365/403 (90.57%), Query Frame = 0

Query: 60  IMVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPL 119
           + VKEK T ERKWKT +V+ +DHI +P FP +LS YEYDAYFDEY+TKTATKPFSQ+IPL
Sbjct: 1   MQVKEK-TEERKWKTTKVSLKDHICIPNFPTELSSYEYDAYFDEYSTKTATKPFSQAIPL 60

Query: 120 WEIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQP 179
           WEIHVFNYPT+HA CS+IFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSR+ SKQP
Sbjct: 61  WEIHVFNYPTTHATCSVIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQP 120

Query: 180 KNDARFGLLSHLPRR----ISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAIS 239
           KN   F  LSH P R    +S+ V NFGWS+MKNTFVEDDPTPIKP+ D MQLVKPIAIS
Sbjct: 121 KNKLDFCRLSHFPARFFSSVSNFVLNFGWSIMKNTFVEDDPTPIKPQEDSMQLVKPIAIS 180

Query: 240 TMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSSALILLNTRKAK 299
           TMTFSLDQIKQIKN LN S+NDVLTGIIF+GVRLYMQEHNP+SSE NSSALILLNTRKAK
Sbjct: 181 TMTFSLDQIKQIKNKLNASVNDVLTGIIFLGVRLYMQEHNPESSEANSSALILLNTRKAK 240

Query: 300 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDS-TLSNNPLEFVKEAKEKIMLQRNTLS 359
           AYKSVKEM++ D+DAPWGN+IAFLPVPIPKLIDS  +S+ PLEFV++ KEKI LQR+ LS
Sbjct: 241 AYKSVKEMVKKDSDAPWGNKIAFLPVPIPKLIDSPVVSSTPLEFVEKVKEKITLQRSPLS 300

Query: 360 VFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVP 419
           VF+AAKVF+I+ +VTGPE+GAKLFKRKLKNSSIMIS++IGP+EKMAL NRPVKGLYFTVP
Sbjct: 301 VFLAAKVFEILKNVTGPEIGAKLFKRKLKNSSIMISNLIGPVEKMALVNRPVKGLYFTVP 360

Query: 420 GMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKR 458
           GMPQSL+ITIVSYMGDLRIVFGGEKCFI+QQKLKVCIEDAF+R
Sbjct: 361 GMPQSLMITIVSYMGDLRIVFGGEKCFINQQKLKVCIEDAFQR 402

BLAST of Moc04g01510 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 173.3 bits (438), Expect = 4.5e-43
Identity = 132/501 (26.35%), Postives = 235/501 (46.91%), Query Frame = 0

Query: 4   LEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVK 63
           +E+   P+SP ++ F+  E    ++ V+ L++ I P   I  +   L+  +PRF+S +V 
Sbjct: 21  VEEEEQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVN 80

Query: 64  --EKSTGERKWKTAEVNPRDHIYVPTFPND-LSPYEYDAYFDEYATKTATKPFSQSIPLW 123
               +  E+KW    V   DH+ +P      +     D + + Y +   T P   S PLW
Sbjct: 81  NCNNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLW 140

Query: 124 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 183
           E+H+ +  TS A    + ++HHS+ DG  +M+ +L+C ++  +P+     PS  +  +P 
Sbjct: 141 EVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPN---ELPSLPYQNRPS 200

Query: 184 NDARFGLLSHLPRRISSVVSNFGWSVM---------KNT------------FVEDDPTPI 243
           +       S   +  S   S F W VM          NT            F++D  TPI
Sbjct: 201 SG------SSSLKTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPI 260

Query: 244 KPEGDC-MQLVKPIAISTMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEH--NP 303
           K  GD  +   K + +   T SLD IK IKN + +++NDV+ G+   G+  Y++      
Sbjct: 261 K--GDFKLSKGKRMCMVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQ 320

Query: 304 QSSEKNSS---------ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLI 363
           + S++NSS         A +L+N R     + + +M+   +   WGN I ++  P     
Sbjct: 321 EESKRNSSNIPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFP----F 380

Query: 364 DSTLSNNPLEFVKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSI 423
              L ++PL+ ++ AK  I  ++N+L   +   V  I+ +  G +  A +  R L N+++
Sbjct: 381 SIALCDDPLKHLRRAKSTIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTM 440

Query: 424 MISSMIGPLEKMALANRPVKGLYFTVPGMPQSLLITIVSYMGDLRIVFGGEKCFI-DQQK 468
             S+++GP+E+++     V  +  +V G P +L +   SYM  L I    +   I D  K
Sbjct: 441 SFSNLVGPVEEISFYGHTVTYIAPSVYGHPHALTMHFQSYMNKLTISLTVDPTVISDPHK 500

BLAST of Moc04g01510 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 169.5 bits (428), Expect = 6.5e-42
Identity = 123/491 (25.05%), Postives = 234/491 (47.66%), Query Frame = 0

Query: 5   EDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKE 64
           ++   P+SP ++ F+  E   +++ V+  +S + P   I    +  +  +PRF+S +V +
Sbjct: 14  KEEEQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFI-RHPRFSSKLVTD 73

Query: 65  KSTGERKWKTAEVNPRDHIYVPTF-PNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIH 124
           ++   ++W    V   DH  VP   P ++     +A+ ++Y +     P   S PLWE+H
Sbjct: 74  ENGQNQRWVRTNVVVEDHFIVPKIKPQNIE--NSNAFLEDYVSDLMKIPLDTSRPLWELH 133

Query: 125 VFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADD----PSLPLTFPSRKHSK-- 184
           + +  TS A    + K+HHS+ DG  +M+ +L+C+++  +    PSLP  + S   S   
Sbjct: 134 LLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLL 193

Query: 185 --QPKNDARFGLL-----SHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQL-V 244
               ++D+R   L     + +   +++V     + ++   FV+D  TPIK  GD +    
Sbjct: 194 TTGSRSDSRLLWLVKVIWTAVILGLNTVCDALEF-IVTTLFVKDTETPIK--GDFLSTKS 253

Query: 245 KPIAISTMTFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYMQEHNPQSSEKNSS----- 304
           K + +   T SLD IK  KN +N++INDV+ G+   G+  Y+     +   KN       
Sbjct: 254 KQLRLVHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLP 313

Query: 305 ------ALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEF 364
                 + +L+N R     + + +M+E  +   WGN   ++  P        L ++PLE 
Sbjct: 314 KRIRLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFP----FSIALRDDPLEH 373

Query: 365 VKEAKEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEK 424
           ++ A++ I  ++N+    +      I+  + G +V A +  R + N+++  S+M+GP+E+
Sbjct: 374 LEIAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEE 433

Query: 425 MALANRPVKGLYFTVPGMPQSLLITIVSYMGDLRIVFGGEKCFI-DQQKLKVCIEDAFKR 469
           ++    P+     +  G P +L I   SYM  + I    +   I D  +L    E++ + 
Sbjct: 434 VSFYGHPITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPHRLCDDWEESLRS 493

BLAST of Moc04g01510 vs. TAIR 10
Match: AT2G38995.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.6e-40
Identity = 120/438 (27.40%), Postives = 216/438 (49.32%), Query Frame = 0

Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP+SQ F +  +   ++  L  ++  +P   +  V +  +   PRF+S  V+ K  G+
Sbjct: 22  PLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKL-PRFSS-KVEIKKNGK 81

Query: 70  RKWKTAEVNPRDHIYVPTFPNDLSPYEY-DAYFDEYATKTATKPFSQSIPLWEIHVFNYP 129
             W    V   DH+ VP    D S  E  D + ++Y +K A  P   S PLWE+H+ N  
Sbjct: 82  ASWVPVSVRVEDHVVVPDL--DYSNIENPDQFIEDYTSKLANTPMDMSRPLWELHLLNIK 141

Query: 130 TSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDP-SLPLTFPSRKH-SKQPKNDARFG 189
           TS+A    I K HHS+ DG  L++ LL+  ++  DP +LP T  +RKH S   K+    G
Sbjct: 142 TSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKKSWWLVG 201

Query: 190 LLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPE-GDCMQLVKPIAISTMTFSLDQIK 249
               + R I + V      ++   F+ D  TP+  + GD ++  K I       S D +K
Sbjct: 202 RFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLMGKTGDAIRSRKVI---HRIVSFDDVK 261

Query: 250 QIKNNLNVSINDVLTGIIFIGV-----RLYMQEHNPQSSEKNSSAL-----ILLNTRKAK 309
            +KNN+++ +NDVL G+   G+     R Y+ E      +KN   +     + +N R   
Sbjct: 262 LVKNNMDMKVNDVLLGMTQAGLSRYLSRKYVDEDMVVEKKKNLEKIRLRGTVFVNLRADT 321

Query: 310 AYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSV 369
             + +  M+   +   WGN +  +  P+   + S   ++PLE+V+ AK  + +++ ++  
Sbjct: 322 KLEDLANMMAKGSKCRWGNFVGVIIFPL--WVRS--EDDPLEYVRRAKSTMDIKKLSIES 381

Query: 370 FMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPG 429
            +   +  +   + G +V   L +R   ++++  S+++GP E ++  + P+  +  +  G
Sbjct: 382 LICYGLIKLTRKILGGKVVETLVRRLFDHTTLTFSNVMGPDEDISFFDHPMSYVAASALG 441

Query: 430 MPQSLLITIVSYMGDLRI 434
            PQ+L+I  V+Y+  + I
Sbjct: 442 GPQALIIHYVTYVNKIVI 448

BLAST of Moc04g01510 vs. TAIR 10
Match: AT5G12420.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 162.5 bits (410), Expect = 8.0e-40
Identity = 125/481 (25.99%), Postives = 223/481 (46.36%), Query Frame = 0

Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVL---LPANPRFTSIMVKEKS 69
           P+SP+++ F +  + +  + ++  ++ I+P     +V D L   +  +PRF+SI+    S
Sbjct: 8   PVSPMARVFQSPGIDLCAVTIMGFKTKINP----DVVLDALKQNVSKHPRFSSIL----S 67

Query: 70  TGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLWEIHVFN 129
               KW   EVN  DH+ VP    +       ++ D+Y ++    P  +S PLW+IH+ N
Sbjct: 68  DNGAKWIETEVNVEDHVIVPYIDAEEIGEGGQSFIDDYMSRLTMIPLDRSRPLWDIHILN 127

Query: 130 YPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSL-PLTFPSRK------HSKQP 189
             TS A      + HHS+ DG  L++ +L+C  +  DP +     PS K      HS + 
Sbjct: 128 VKTSEAEAVGFIRSHHSLGDGMSLISLMLACTHKTSDPDMFSNAIPSMKRRATMSHSLKT 187

Query: 190 KNDARFGL--LSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTM 249
           K      +  +    R + +   +    +    F++D  TP+K   D      P      
Sbjct: 188 KGWFLRSIFTIGSTMRLLWNTTIDMLLLLATVLFLKDTKTPLKAGADVRS--NPKRFYHR 247

Query: 250 TFSLDQIKQIKNNLNVSINDVLTGIIFIGVRLYM--------QEHNPQSSEKNS------ 309
             SLD IK IKN +N++INDVL GI    +  Y+        +E    +S +N+      
Sbjct: 248 IISLDDIKLIKNAMNMTINDVLFGITQASLSHYLNRQYGTKKEEDGALTSYRNNLPDGIR 307

Query: 310 -SALILLNTRKAKAYKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEA 369
                 +N R    +K + +M+  D+   WGN  +F+ +P        L  +PL ++K +
Sbjct: 308 FRVACTVNLRSDIGFKPLADMMVKDSKCRWGNYFSFIFLP----FTIGLQTDPLVYLKMS 367

Query: 370 KEKIMLQRNTLSVFMAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALA 429
           K  +  ++++    +   +  IV  V G +  A+LF R ++N++  +S++IGP+E+++  
Sbjct: 368 KSMMARKKHSYHAALVYFIIKIVLKVFGAKAAAELFDRPVRNTTTCVSNVIGPMEEISFR 427

Query: 430 NRPVKGLYFTVPGMPQSLLITIVSYMGDLRIVFGGEKCFIDQQKLKVCIEDAFKRTLMKA 464
             PV  +  +  G   +LLI ++SY   + I    +   I     K+C +       MKA
Sbjct: 428 GHPVSYIAPSSYGHSHALLIHLMSYADKMIISLAYDPTVISDPH-KICDDMEESLKAMKA 473

BLAST of Moc04g01510 vs. TAIR 10
Match: AT2G38995.2 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 162.2 bits (409), Expect = 1.0e-39
Identity = 119/437 (27.23%), Postives = 215/437 (49.20%), Query Frame = 0

Query: 10  PMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSIMVKEKSTGE 69
           P+SP+SQ F +  +   ++  L  ++  +P   +  V +  +   PRF+S  V+ K  G+
Sbjct: 22  PLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKL-PRFSS-KVEIKKNGK 81

Query: 70  RKWKTAEVNPRDHIYVPTFPNDLSPYEY-DAYFDEYATKTATKPFSQSIPLWEIHVFNYP 129
             W    V   DH+ VP    D S  E  D + ++Y +K A  P   S PLWE+H+ N  
Sbjct: 82  ASWVPVSVRVEDHVVVPDL--DYSNIENPDQFIEDYTSKLANTPMDMSRPLWELHLLNIK 141

Query: 130 TSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDP-SLPLTFPSRKH-SKQPKNDARFG 189
           TS+A    I K HHS+ DG  L++ LL+  ++  DP +LP T  +RKH S   K+    G
Sbjct: 142 TSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASSNKKSWWLVG 201

Query: 190 LLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPE-GDCMQLVKPIAISTMTFSLDQIK 249
               + R I + V      ++   F+ D  TP+  + GD ++  K I       S D +K
Sbjct: 202 RFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPLMGKTGDAIRSRKVI---HRIVSFDDVK 261

Query: 250 QIKNNLNVSINDVLTGIIFIGVRLYM----QEHNPQSSEKNSSAL-----ILLNTRKAKA 309
            +KNN+++ +NDVL G+   G+  Y+     E      +KN   +     + +N R    
Sbjct: 262 LVKNNMDMKVNDVLLGMTQAGLSRYLSRKYDEDMVVEKKKNLEKIRLRGTVFVNLRADTK 321

Query: 310 YKSVKEMLETDTDAPWGNRIAFLPVPIPKLIDSTLSNNPLEFVKEAKEKIMLQRNTLSVF 369
            + +  M+   +   WGN +  +  P+   + S   ++PLE+V+ AK  + +++ ++   
Sbjct: 322 LEDLANMMAKGSKCRWGNFVGVIIFPL--WVRS--EDDPLEYVRRAKSTMDIKKLSIESL 381

Query: 370 MAAKVFDIVNHVTGPEVGAKLFKRKLKNSSIMISSMIGPLEKMALANRPVKGLYFTVPGM 429
           +   +  +   + G +V   L +R   ++++  S+++GP E ++  + P+  +  +  G 
Sbjct: 382 ICYGLIKLTRKILGGKVVETLVRRLFDHTTLTFSNVMGPDEDISFFDHPMSYVAASALGG 441

Query: 430 PQSLLITIVSYMGDLRI 434
           PQ+L+I  V+Y+  + I
Sbjct: 442 PQALIIHYVTYVNKIVI 447

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147882.12.3e-267100.00O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransfer... [more]
XP_011659709.24.9e-21781.01O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_01... [more]
XP_022952997.11.2e-20779.14O-acyltransferase WSD1-like [Cucurbita moschata][more]
XP_038887587.12.3e-19078.35O-acyltransferase WSD1-like isoform X2 [Benincasa hispida][more]
XP_038887586.13.9e-19078.54O-acyltransferase WSD1-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9M3B26.4e-4226.35Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B39.2e-4125.05Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3... [more]
Q94CK01.1e-3825.99Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3... [more]
F4IU141.5e-3827.23Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B11.5e-3826.25Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1D2I71.1e-267100.00Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 ... [more]
A0A6J1GLZ35.9e-20879.14Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 P... [more]
A0A0A0K2E95.5e-19080.43Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051330 PE=4... [more]
A0A5D3C6U27.5e-18781.39Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7SPR91.7e-18681.14Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT3G49200.14.5e-4326.35O-acyltransferase (WSD1-like) family protein [more]
AT3G49190.16.5e-4225.05O-acyltransferase (WSD1-like) family protein [more]
AT2G38995.13.6e-4027.40O-acyltransferase (WSD1-like) family protein [more]
AT5G12420.18.0e-4025.99O-acyltransferase (WSD1-like) family protein [more]
AT2G38995.21.0e-3927.23O-acyltransferase (WSD1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004255O-acyltransferase, WSD1, N-terminalPFAMPF03007WES_acyltransfcoord: 52..258
e-value: 1.7E-9
score: 37.8
IPR009721O-acyltransferase WSD1, C-terminalPFAMPF06974WS_DGAT_Ccoord: 312..456
e-value: 5.2E-33
score: 114.0
NoneNo IPR availablePANTHERPTHR31650:SF34O-ACYLTRANSFERASE WSD1-LIKEcoord: 6..464
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 6..464

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g01510.1Moc04g01510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0045017 glycerolipid biosynthetic process
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0047196 long-chain-alcohol O-fatty-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity