Moc04g00990 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g00990
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like
Location	chr4: 679381 .. 680177 (+)
RNA-Seq Expression	Moc04g00990
Synteny	Moc04g00990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGATCTTCAAATTCGGCCGCTGTATGCTCTTCCTCCTCCTGATTCCCCTCTGCCACGCGGCGCCGTCGATCAGAGATCAAGGCGGCGCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCCTCTCTGCAGTCGGCCCTCGACTGGGCGTGCGGCGCCGGCGGCGCCGACTGCTCCCCAATCCAGGCCGGCGGGCCCTGCTACGACTCCTCTGACGTCCAGCACATGGCCTCCTTCGCCTTCAACGATTACTTCCTCAAGCACGGCGTGACCGACGACAGCTGCTTCTTCCAAAACGCCGCCGCTCTCACCTCCCTAAACCCTAGTCAGTATAATCTTTCAAATTTCTCGATTCAATCTCTTCTTTCGCTCAAATTCATCCCAGATCTAAAAGCTCGAGTTTGATTTTGATGTTTCTTGTGTTTCAGGCTATGGAAATTGCAAATTCCCTTCCAGGTAAATTCGTCGATCGATCTTTAAAACCCTGATTCGATTTGGAGCTTAATTAAATTTAATTTCCAAATCAAGAAATAAACACGTGGGTCACGGTGACTCACCAATGAATCTGCAACCCTTTTTCGAGCTTAATGGGTTTTGATTTCTCTCATTTATGGCGCAGTTTGTTGAGAAATGGAAACTTTTCGAGCCAAACTACAGCGGTAGGGTTGGGGCCAAGCGAAGACATAAGCGGGTCGGATCTCAATGCGGGCCGGGTACGGGTTTTGCATATGGTAATCGGGTATTTGTTTTTCATAATTATATTTCAAAGAGGAAATTTCCTCTAG

mRNA sequence

ATGCCGATCTTCAAATTCGGCCGCTGTATGCTCTTCCTCCTCCTGATTCCCCTCTGCCACGCGGCGCCGTCGATCAGAGATCAAGGCGGCGCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCCTCTCTGCAGTCGGCCCTCGACTGGGCGTGCGGCGCCGGCGGCGCCGACTGCTCCCCAATCCAGGCCGGCGGGCCCTGCTACGACTCCTCTGACGTCCAGCACATGGCCTCCTTCGCCTTCAACGATTACTTCCTCAAGCACGGCGTGACCGACGACAGCTGCTTCTTCCAAAACGCCGCCGCTCTCACCTCCCTAAACCCTAGCTATGGAAATTGCAAATTCCCTTCCAGTTTGTTGAGAAATGGAAACTTTTCGAGCCAAACTACAGCGGTAGGGTTGGGGCCAAGCGAAGACATAAGCGGGTCGGATCTCAATGCGGGCCGGGTACGGGTTTTGCATATGGTAATCGGGTATTTGTTTTTCATAATTATATTTCAAAGAGGAAATTTCCTCTAG

Coding sequence (CDS)

ATGCCGATCTTCAAATTCGGCCGCTGTATGCTCTTCCTCCTCCTGATTCCCCTCTGCCACGCGGCGCCGTCGATCAGAGATCAAGGCGGCGCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCCTCTCTGCAGTCGGCCCTCGACTGGGCGTGCGGCGCCGGCGGCGCCGACTGCTCCCCAATCCAGGCCGGCGGGCCCTGCTACGACTCCTCTGACGTCCAGCACATGGCCTCCTTCGCCTTCAACGATTACTTCCTCAAGCACGGCGTGACCGACGACAGCTGCTTCTTCCAAAACGCCGCCGCTCTCACCTCCCTAAACCCTAGCTATGGAAATTGCAAATTCCCTTCCAGTTTGTTGAGAAATGGAAACTTTTCGAGCCAAACTACAGCGGTAGGGTTGGGGCCAAGCGAAGACATAAGCGGGTCGGATCTCAATGCGGGCCGGGTACGGGTTTTGCATATGGTAATCGGGTATTTGTTTTTCATAATTATATTTCAAAGAGGAAATTTCCTCTAG

Protein sequence

MPIFKFGRCMLFLLLIPLCHAAPSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFPSSLLRNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQRGNFL

Homology

[Close]

BLAST of Moc04g00990 vs. NCBI nr
Match: XP_022147980.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Momordica charantia])

HSP 1 Score: 362.8 bits (930), Expect = 1.6e-96
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MPIFKFGRCMLFLLLIPLCHAAPSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGA 60
           MPIFKFGRCMLFLLLIPLCHAAPSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGA
Sbjct: 1   MPIFKFGRCMLFLLLIPLCHAAPSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGA 60

Query: 61  DCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFP 120
           DCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFP
Sbjct: 61  DCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFP 120

Query: 121 SSLLRNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQRGNFL 177
           SSLLRNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQRGNFL
Sbjct: 121 SSLLRNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQRGNFL 176

[Close]

BLAST of Moc04g00990 vs. NCBI nr
Match: XP_038877601.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Benincasa hispida])

HSP 1 Score: 262.3 bits (669), Expect = 3.0e-66
Identity = 136/173 (78.61%), Postives = 148/173 (85.55%), Query Frame = 0


Query: 11  LFLLLIPLCHAA----PSIRDQGG-ATELWCVAKNNADDASLQSALDWACGAGGADCSPI 70
           L +LLIP C AA    PSIRDQ G ATELWCVAKNNADDASLQSALDWACGAGGADCSPI
Sbjct: 67  LLILLIP-CTAADPRRPSIRDQSGVATELWCVAKNNADDASLQSALDWACGAGGADCSPI 126

Query: 71  QAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFPSSLLR 130
           Q GG CYD++DVQ+MASFAFNDYF KHG+TDDSCFFQN+AA+TSLNPSYGNC+FPSS+LR
Sbjct: 127 QPGGACYDAADVQNMASFAFNDYFRKHGMTDDSCFFQNSAAMTSLNPSYGNCRFPSSVLR 186

Query: 131 NGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIII--FQRGNFL 177
           NGNFSSQTT+VGLGPSEDISGS  N+ R     MVIGYLF  I+  FQRGN L
Sbjct: 187 NGNFSSQTTSVGLGPSEDISGSSRNSARAWAWPMVIGYLFLRIMFQFQRGNLL 238

[Close]

BLAST of Moc04g00990 vs. NCBI nr
Match: XP_022989094.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 258.1 bits (658), Expect = 5.6e-65
Identity = 128/165 (77.58%), Postives = 145/165 (87.88%), Query Frame = 0


Query: 11  LFLLLIP-LCHAA----PSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGADCSPI 70
           LFLL++P LC AA    PSI+ QGGATELWCVAKNNADDASLQSALDWACGAGGADCSPI
Sbjct: 7   LFLLIVPILCSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGGADCSPI 66

Query: 71  QAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFPSSLLR 130
           Q  G CYD+S+VQ+MASFAFNDYFLKHG+TDDSCFFQN+AA+TSLNPSYGNC+FPSS LR
Sbjct: 67  QPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAVTSLNPSYGNCRFPSSSLR 126

Query: 131 NGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIF 171
           NGNFSSQ+T+VGLGPSED+SGS   + R+ V  + IGYLFF I+F
Sbjct: 127 NGNFSSQSTSVGLGPSEDLSGSGRRSSRIWVWPIAIGYLFFKIMF 171

[Close]

BLAST of Moc04g00990 vs. NCBI nr
Match: XP_023554742.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 254.6 bits (649), Expect = 6.2e-64
Identity = 127/167 (76.05%), Postives = 142/167 (85.03%), Query Frame = 0


Query: 11  LFLLLIPLCHAA------PSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGADCSP 70
           LFLL++ +  ++      PS RDQ GATELWCVAKNNADDASLQSALDWACGAGGADCSP
Sbjct: 7   LFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAGGADCSP 66

Query: 71  IQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFPSSLL 130
           IQ GG CYD+S+VQ+MASFAFNDYF KHG+TDDSCFFQNAAA+TSLNPSYGNC+ P+S L
Sbjct: 67  IQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCRIPASSL 126

Query: 131 RNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQ 172
           RNGNFSSQT +VGLGPSEDISGS   +GR  V  MVIGYLFF I+FQ
Sbjct: 127 RNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQ 173

[Close]

BLAST of Moc04g00990 vs. NCBI nr
Match: XP_022952645.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita moschata])

HSP 1 Score: 254.6 bits (649), Expect = 6.2e-64
Identity = 127/167 (76.05%), Postives = 142/167 (85.03%), Query Frame = 0


Query: 11  LFLLLIPLCHAA------PSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGADCSP 70
           LFLL++ +  ++      PS RDQ GATELWCVAKNNADDASLQSALDWACGAGGADCSP
Sbjct: 7   LFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAGGADCSP 66

Query: 71  IQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFPSSLL 130
           IQ GG CYD+S+VQ+MASFAFNDYF KHG+TDDSCFFQNAAA+TSLNPSYGNC+ P+S L
Sbjct: 67  IQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCRIPASSL 126

Query: 131 RNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQ 172
           RNGNFSSQT +VGLGPSEDISGS   +GR  V  MVIGYLFF I+FQ
Sbjct: 127 RNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQ 173

[Close]

BLAST of Moc04g00990 vs. ExPASy Swiss-Prot
Match: Q9M2K6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5 PE=2 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 8.2e-35
Identity = 68/110 (61.82%), Postives = 85/110 (77.27%), Query Frame = 0


Query: 33  ELWCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLKH 92
           ELWCVAKNNA+D+SLQ+A++WACG GGADC PIQ GGPC D +DVQ MASF FN+Y+LK+
Sbjct: 39  ELWCVAKNNAEDSSLQTAIEWACGQGGADCGPIQQGGPCNDPTDVQKMASFVFNNYYLKN 98

Query: 93  GVTDDSCFFQNAAALTSLNPSYGNCKFPSSLLRNGNFSSQTTAVGLGPSE 143
           G  D++C F N AALTSLNPS G CK+PSS   N    +  T++G G ++
Sbjct: 99  GEEDEACNFNNNAALTSLNPSQGTCKYPSSKGANNGRLADDTSMGAGQAD 148

[Close]

BLAST of Moc04g00990 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 8.3e-19
Identity = 55/133 (41.35%), Postives = 74/133 (55.64%), Query Frame = 0


Query: 32  TELWCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLK 91
           + ++CVAK +ADD  L   L+WACG G A+C+ IQ G PCY  +DV+  ASFAFNDY+ K
Sbjct: 359 SSMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQK 418

Query: 92  HGVTDDSCFFQNAAALTSLNPSYGNCKFPSSL---LRNGNFSSQTTAVGLGPSEDISGSD 151
                 +C F   A  T+ +PSY  C +  SL     NGNF        LGP+  + G +
Sbjct: 419 MKSAGGTCDFDGTAITTTRDPSYRTCAYTGSLNANATNGNFPPD----ALGPASPL-GGN 478

Query: 152 LNAGRVRVLHMVI 162
            NA  +   H+ I
Sbjct: 479 ANARIIFSYHLPI 486

[Close]

BLAST of Moc04g00990 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 94.0 bits (232), Expect = 1.8e-18
Identity = 45/114 (39.47%), Postives = 63/114 (55.26%), Query Frame = 0


Query: 33  ELWCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLKH 92
           + +C+AK   D   LQ+ALDWACG G  DCS +  G  CY+  DV   +++AFN Y+ K 
Sbjct: 358 QTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKM 417

Query: 93  GVTDDSCFFQNAAALTSLNPSYGNCKFPSSLLRNGNFSSQTTAVGLGPSEDISG 147
           G    SC F+  A +T+ +PS G C FP S   N    + T+A+    +   SG
Sbjct: 418 GKASGSCDFKGVATVTTTDPSRGTCVFPGSAKSNQTLGNNTSALAPSANSTTSG 471

[Close]

BLAST of Moc04g00990 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 93.2 bits (230), Expect = 3.1e-18
Identity = 42/90 (46.67%), Postives = 60/90 (66.67%), Query Frame = 0


Query: 33  ELWCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLKH 92
           + +C+A +  D  +LQ+ALDWACG G ++CS IQ G  CY  ++V+  ASFAFN Y+ K 
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKE 438

Query: 93  GVTDDSCFFQNAAALTSLNPSYGNCKFPSS 123
           G    SC F+  A +T+ +PS+G+C FP S
Sbjct: 439 GRASGSCDFKGVAMITTTDPSHGSCIFPGS 468

[Close]

BLAST of Moc04g00990 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 4.1e-18
Identity = 40/91 (43.96%), Postives = 54/91 (59.34%), Query Frame = 0


Query: 29  GGATELWCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDY 88
           GG T+ WC+A + A    LQ+ALDWACG G  DCS +Q   PC++   V   AS+AFN Y
Sbjct: 385 GGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTY 444

Query: 89  FLKHGVTDDSCFFQNAAALTSLNPSYGNCKF 120
           + + G +   C F  A+     +PSYGNC +
Sbjct: 445 YQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

[Close]

BLAST of Moc04g00990 vs. ExPASy TrEMBL
Match: A0A6J1D1N4 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Momordica charantia OX=3673 GN=LOC111016774 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 7.9e-97
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MPIFKFGRCMLFLLLIPLCHAAPSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGA 60
           MPIFKFGRCMLFLLLIPLCHAAPSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGA
Sbjct: 1   MPIFKFGRCMLFLLLIPLCHAAPSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGA 60

Query: 61  DCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFP 120
           DCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFP
Sbjct: 61  DCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFP 120

Query: 121 SSLLRNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQRGNFL 177
           SSLLRNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQRGNFL
Sbjct: 121 SSLLRNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQRGNFL 176

[Close]

BLAST of Moc04g00990 vs. ExPASy TrEMBL
Match: A0A6J1JNE0 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486269 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.7e-65
Identity = 128/165 (77.58%), Postives = 145/165 (87.88%), Query Frame = 0


Query: 11  LFLLLIP-LCHAA----PSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGADCSPI 70
           LFLL++P LC AA    PSI+ QGGATELWCVAKNNADDASLQSALDWACGAGGADCSPI
Sbjct: 7   LFLLIVPILCSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGGADCSPI 66

Query: 71  QAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFPSSLLR 130
           Q  G CYD+S+VQ+MASFAFNDYFLKHG+TDDSCFFQN+AA+TSLNPSYGNC+FPSS LR
Sbjct: 67  QPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAVTSLNPSYGNCRFPSSSLR 126

Query: 131 NGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIF 171
           NGNFSSQ+T+VGLGPSED+SGS   + R+ V  + IGYLFF I+F
Sbjct: 127 NGNFSSQSTSVGLGPSEDLSGSGRRSSRIWVWPIAIGYLFFKIMF 171

[Close]

BLAST of Moc04g00990 vs. ExPASy TrEMBL
Match: A0A6J1GMC7 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita moschata OX=3662 GN=LOC111455276 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 3.0e-64
Identity = 127/167 (76.05%), Postives = 142/167 (85.03%), Query Frame = 0


Query: 11  LFLLLIPLCHAA------PSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGADCSP 70
           LFLL++ +  ++      PS RDQ GATELWCVAKNNADDASLQSALDWACGAGGADCSP
Sbjct: 7   LFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAGGADCSP 66

Query: 71  IQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFPSSLL 130
           IQ GG CYD+S+VQ+MASFAFNDYF KHG+TDDSCFFQNAAA+TSLNPSYGNC+ P+S L
Sbjct: 67  IQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCRIPASSL 126

Query: 131 RNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIFQ 172
           RNGNFSSQT +VGLGPSEDISGS   +GR  V  MVIGYLFF I+FQ
Sbjct: 127 RNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQ 173

[Close]

BLAST of Moc04g00990 vs. ExPASy TrEMBL
Match: A0A6J1EY09 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437228 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 8.8e-64
Identity = 126/165 (76.36%), Postives = 143/165 (86.67%), Query Frame = 0


Query: 11  LFLLL-IPLCHAA----PSIRDQGGATELWCVAKNNADDASLQSALDWACGAGGADCSPI 70
           LFLL+ + +C AA    PSI+ QGGATELWCVAKNNADDASLQSALDWACGAGG+DCSPI
Sbjct: 7   LFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGGSDCSPI 66

Query: 71  QAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFPSSLLR 130
           Q  G CYD+S VQ MASFAFNDYFLKHG+TDDSCFFQN+AA+TSLNPSYGNC+FPSS LR
Sbjct: 67  QPSGACYDASSVQTMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLR 126

Query: 131 NGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIIIF 171
           NGNFSSQ+T+VGLGPSED+SGS   +GR+ V  + IGYLFF I+F
Sbjct: 127 NGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMF 171

[Close]

BLAST of Moc04g00990 vs. ExPASy TrEMBL
Match: A0A1S3BN12 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Cucumis melo OX=3656 GN=LOC103491346 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.6e-63
Identity = 129/165 (78.18%), Postives = 142/165 (86.06%), Query Frame = 0


Query: 11  LFLLLIPLCHAA-----PSIRDQ-GGATELWCVAKNNADDASLQSALDWACGAGGADCSP 70
           L +LLIP C AA     PSIRDQ G ATELWCVAKNNADDASLQSALDWACGAGGADCSP
Sbjct: 9   LLILLIP-CTAADPWRRPSIRDQSGAATELWCVAKNNADDASLQSALDWACGAGGADCSP 68

Query: 71  IQAGGPCYDSSDVQHMASFAFNDYFLKHGVTDDSCFFQNAAALTSLNPSYGNCKFPSSLL 130
           IQ GG CYD++DVQ+MASFAFNDYF KHG+TDDSCFFQN+AA+TSLNPS+GNC+FPSS+L
Sbjct: 69  IQPGGSCYDATDVQNMASFAFNDYFRKHGMTDDSCFFQNSAAITSLNPSFGNCRFPSSVL 128

Query: 131 RNGNFSSQTTAVGLGPSEDISGSDLNAGRVRVLHMVIGYLFFIII 170
           RNGNFSSQTT+VGLGPSED+SGS  N GR  V  MVIGYL   I+
Sbjct: 129 RNGNFSSQTTSVGLGPSEDMSGSAHNLGRAWVWPMVIGYLLLRIM 172

[Close]

BLAST of Moc04g00990 vs. TAIR 10
Match: AT3G58100.1 (plasmodesmata callose-binding protein 5 )

HSP 1 Score: 148.3 bits (373), Expect = 5.9e-36
Identity = 68/110 (61.82%), Postives = 85/110 (77.27%), Query Frame = 0


Query: 33  ELWCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLKH 92
           ELWCVAKNNA+D+SLQ+A++WACG GGADC PIQ GGPC D +DVQ MASF FN+Y+LK+
Sbjct: 39  ELWCVAKNNAEDSSLQTAIEWACGQGGADCGPIQQGGPCNDPTDVQKMASFVFNNYYLKN 98

Query: 93  GVTDDSCFFQNAAALTSLNPSYGNCKFPSSLLRNGNFSSQTTAVGLGPSE 143
           G  D++C F N AALTSLNPS G CK+PSS   N    +  T++G G ++
Sbjct: 99  GEEDEACNFNNNAALTSLNPSQGTCKYPSSKGANNGRLADDTSMGAGQAD 148

[Close]

BLAST of Moc04g00990 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.1e-21
Identity = 50/88 (56.82%), Postives = 59/88 (67.05%), Query Frame = 0


Query: 35  WCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHGV 94
           WC+AK NA   SLQ ALD+ACG GGADC  IQ G  CY+ + ++  ASFAFN Y+ KH  
Sbjct: 148 WCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFNSYYQKH-P 207

Query: 95  TDDSCFFQNAAALTSLNPSYGNCKFPSS 123
             DSC F  AA LTS +PS G+C F SS
Sbjct: 208 GSDSCNFGGAAQLTSTDPSKGSCHFSSS 234

[Close]

BLAST of Moc04g00990 vs. TAIR 10
Match: AT4G13600.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 6.9e-21
Identity = 51/120 (42.50%), Postives = 73/120 (60.83%), Query Frame = 0


Query: 34  LWCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHG 93
           +WCVA+ +    +LQ+ALD+AC A GADC+PIQ  G C+  + VQ  AS+AFN YF +  
Sbjct: 60  MWCVARFDVTSQALQAALDYAC-AAGADCAPIQPNGLCFLPNTVQAHASYAFNSYFQRAA 119

Query: 94  VTDDSCFFQNAAALTSLNPSYGNCKFPSSLLRNGNFSSQTTAVGLGPSEDISGSDLNAGR 153
           +   SC F   + +   +PSYG+C +P+S +RN   S+ TT VG  PS  +  S +   R
Sbjct: 120 MAPGSCNFAGTSTIAKTDPSYGSCVYPNS-VRNAGGSASTTTVGGTPSTTVGNSPMTTLR 177

[Close]

BLAST of Moc04g00990 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 96.3 bits (238), Expect = 2.6e-20
Identity = 44/87 (50.57%), Postives = 54/87 (62.07%), Query Frame = 0


Query: 35  WCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLKHGV 94
           WCVA  +A +  LQ  LD+ACG GGADC PIQ G  CY    ++  ASFAFN Y+ K G 
Sbjct: 384 WCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYSPDTLEAHASFAFNSYYQKKGR 443

Query: 95  TDDSCFFQNAAALTSLNPSYGNCKFPS 122
              SC+F  AA + S  P YG C+FP+
Sbjct: 444 AGGSCYFGGAAYVVSQPPKYGRCEFPT 470

[Close]

BLAST of Moc04g00990 vs. TAIR 10
Match: AT3G13560.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 95.1 bits (235), Expect = 5.9e-20
Identity = 55/133 (41.35%), Postives = 74/133 (55.64%), Query Frame = 0


Query: 32  TELWCVAKNNADDASLQSALDWACGAGGADCSPIQAGGPCYDSSDVQHMASFAFNDYFLK 91
           + ++CVAK +ADD  L   L+WACG G A+C+ IQ G PCY  +DV+  ASFAFNDY+ K
Sbjct: 359 SSMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQK 418

Query: 92  HGVTDDSCFFQNAAALTSLNPSYGNCKFPSSL---LRNGNFSSQTTAVGLGPSEDISGSD 151
                 +C F   A  T+ +PSY  C +  SL     NGNF        LGP+  + G +
Sbjct: 419 MKSAGGTCDFDGTAITTTRDPSYRTCAYTGSLNANATNGNFPPD----ALGPASPL-GGN 478

Query: 152 LNAGRVRVLHMVI 162
            NA  +   H+ I
Sbjct: 479 ANARIIFSYHLPI 486

The following BLAST results are available for this feature:

BLAST of Moc04g00990 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147980.1	1.6e-96	100.00	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Momordica charantia]	[more]
XP_038877601.1	3.0e-66	78.61	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Benincasa hispida]	[more]
XP_022989094.1	5.6e-65	77.58	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita maxima]	[more]
XP_023554742.1	6.2e-64	76.05	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 [Cucurbita pepo subsp. p...	[more]
XP_022952645.1	6.2e-64	76.05	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita moschata]	[more]

BLAST of Moc04g00990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2K6	8.2e-35	61.82	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5...	[more]
Q94CD8	8.3e-19	41.35	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
Q9ZU91	1.8e-18	39.47	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
O65399	3.1e-18	46.67	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q8VYE5	4.1e-18	43.96	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]

BLAST of Moc04g00990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1N4	7.9e-97	100.00	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1JNE0	2.7e-65	77.58	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita maxima OX=3...	[more]
A0A6J1GMC7	3.0e-64	76.05	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1EY09	8.8e-64	76.36	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita moschata OX...	[more]
A0A1S3BN12	2.6e-63	78.18	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Cucumis melo OX=3656 GN=LOC103491346 ...	[more]

BLAST of Moc04g00990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58100.1	5.9e-36	61.82	plasmodesmata callose-binding protein 5	[more]
AT1G29380.1	3.1e-21	56.82	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G13600.1	6.9e-21	42.50	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G05790.1	2.6e-20	50.57	O-Glycosyl hydrolases family 17 protein	[more]
AT3G13560.1	5.9e-20	41.35	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 34..119 e-value: 3.0E-40 score: 149.7
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 34..105 e-value: 6.8E-19 score: 68.3
None	No IPR available	GENE3D	1.20.58.1040		coord: 30..131 e-value: 7.2E-11 score: 44.3
None	No IPR available	PANTHER	PTHR31044:SF80	PLASMODESMATA CALLOSE-BINDING PROTEIN 5	coord: 31..142
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 31..142

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g00990.1	Moc04g00990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009506	plasmodesma