Moc04g00870 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g00870
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	ethylene-responsive transcription factor 3-like
Location	chr4: 599367 .. 600005 (-)
RNA-Seq Expression	Moc04g00870
Synteny	Moc04g00870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGGCGGGGCAGGGCCACAGATCCACCGACGGCTGGGCAGACGACTCATGGACCAGTTAAAGAAGTGCGATTCAGAGGCGTTCGGAAGCGGCCATGGGGCCGGTACGCCGCCGAGATTAGAGATCCCTGGAAGAAGACTCGCGTCTGGCTCGGCACCTTCGATACGGCGGAGGATGCCGCCCGAGCTTACGACGCTGCCGCTCTCTCTCTCCGCGGCCCGAAGGCTAAAACGAACTTCCCTCTGCATCCATCGGCGATCTCTGTTCATCACCAAATCCCATTCTCGCAGGAGTTTCACGACTCCGGCGGACTCTGCAAGATTAGCAACATTTCGGTCAATAGACCTACATCGAGCAGTTTGAGTTCCACCGTGGAGTCTTTCAGCGGGCCTAGGGTTTCCATTCCGGCTCCGTCCGTTCCAAAGCTCCATCGGAAGTCCCTGCCGCCGGTCTCCCCCGACGATTGCCACAGCGACTGTGATTCATCGTCCTCGGTTGTTGATGACAGTGAGGAATATGCACGAACCTCTTCAATCCGCAGAGTGTTGCCGTTCGATCTGAACCTACCTCCTCCACTGCCGCCGCACGACGTCGATTTATCCGGTGATGATCTTCAAGCCACTGCTCTGTGTTTCTGA

mRNA sequence

ATGCGGCGGGGCAGGGCCACAGATCCACCGACGGCTGGGCAGACGACTCATGGACCAGTTAAAGAAGTGCGATTCAGAGGCGTTCGGAAGCGGCCATGGGGCCGGTACGCCGCCGAGATTAGAGATCCCTGGAAGAAGACTCGCGTCTGGCTCGGCACCTTCGATACGGCGGAGGATGCCGCCCGAGCTTACGACGCTGCCGCTCTCTCTCTCCGCGGCCCGAAGGCTAAAACGAACTTCCCTCTGCATCCATCGGCGATCTCTGTTCATCACCAAATCCCATTCTCGCAGGAGTTTCACGACTCCGGCGGACTCTGCAAGATTAGCAACATTTCGGTCAATAGACCTACATCGAGCAGTTTGAGTTCCACCGTGGAGTCTTTCAGCGGGCCTAGGGTTTCCATTCCGGCTCCGTCCGTTCCAAAGCTCCATCGGAAGTCCCTGCCGCCGGTCTCCCCCGACGATTGCCACAGCGACTGTGATTCATCGTCCTCGGTTGTTGATGACAGTGAGGAATATGCACGAACCTCTTCAATCCGCAGAGTGTTGCCGTTCGATCTGAACCTACCTCCTCCACTGCCGCCGCACGACGTCGATTTATCCGGTGATGATCTTCAAGCCACTGCTCTGTGTTTCTGA

Coding sequence (CDS)

ATGCGGCGGGGCAGGGCCACAGATCCACCGACGGCTGGGCAGACGACTCATGGACCAGTTAAAGAAGTGCGATTCAGAGGCGTTCGGAAGCGGCCATGGGGCCGGTACGCCGCCGAGATTAGAGATCCCTGGAAGAAGACTCGCGTCTGGCTCGGCACCTTCGATACGGCGGAGGATGCCGCCCGAGCTTACGACGCTGCCGCTCTCTCTCTCCGCGGCCCGAAGGCTAAAACGAACTTCCCTCTGCATCCATCGGCGATCTCTGTTCATCACCAAATCCCATTCTCGCAGGAGTTTCACGACTCCGGCGGACTCTGCAAGATTAGCAACATTTCGGTCAATAGACCTACATCGAGCAGTTTGAGTTCCACCGTGGAGTCTTTCAGCGGGCCTAGGGTTTCCATTCCGGCTCCGTCCGTTCCAAAGCTCCATCGGAAGTCCCTGCCGCCGGTCTCCCCCGACGATTGCCACAGCGACTGTGATTCATCGTCCTCGGTTGTTGATGACAGTGAGGAATATGCACGAACCTCTTCAATCCGCAGAGTGTTGCCGTTCGATCTGAACCTACCTCCTCCACTGCCGCCGCACGACGTCGATTTATCCGGTGATGATCTTCAAGCCACTGCTCTGTGTTTCTGA

Protein sequence

MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDAARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISNISVNRPTSSSLSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSIRRVLPFDLNLPPPLPPHDVDLSGDDLQATALCF

Homology

[Close]

BLAST of Moc04g00870 vs. NCBI nr
Match: XP_038888524.1 (ethylene-responsive transcription factor 3-like [Benincasa hispida])

HSP 1 Score: 387.1 bits (993), Expect = 9.7e-104
Identity = 195/213 (91.55%), Postives = 196/213 (92.02%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGRATDP TA +T   PVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRATDPLTAAETVREPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISNISVNRPTSSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH QIPFSQEFHDSGG CKISNI VNRPTSSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQQIPFSQEFHDSGGFCKISNIPVNRPTSSS 120

Query: 121 LSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSIR 180
           LSSTVESFSGPRVSIP PSVPKL RKSLP VSPDDCHSDCDSSSSVVDDSEEYA TSSIR
Sbjct: 121 LSSTVESFSGPRVSIPVPSVPKLRRKSLPQVSPDDCHSDCDSSSSVVDDSEEYAHTSSIR 180

Query: 181 RVLPFDLNLPPPL-PPHDVDLSGDDLQATALCF 213
           RVLPFDLNLPPPL PPHDVDLSGDDL ATAL F
Sbjct: 181 RVLPFDLNLPPPLPPPHDVDLSGDDLHATALYF 213

[Close]

BLAST of Moc04g00870 vs. NCBI nr
Match: XP_008449487.1 (PREDICTED: ethylene-responsive transcription factor 3-like [Cucumis melo] >KAA0061657.1 ethylene-responsive transcription factor 3-like [Cucumis melo var. makuwa] >TYK05849.1 ethylene-responsive transcription factor 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 375.9 bits (964), Expect = 2.2e-100
Identity = 189/214 (88.32%), Postives = 197/214 (92.06%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGRATDP TA ++   PVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRATDPLTAPESVQEPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISN-ISVNRPTSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH QIPFSQ+FHDSGG C+ISN I VNRPTSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQQIPFSQQFHDSGGFCEISNIIPVNRPTSS 120

Query: 121 SLSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSI 180
           SLSSTVESFSGPR+SIP PSVPKL RKS P V+PDDCHSDCDSSSSVVDDSEEYA++SSI
Sbjct: 121 SLSSTVESFSGPRLSIPVPSVPKLRRKSQPQVAPDDCHSDCDSSSSVVDDSEEYAQSSSI 180

Query: 181 RRVLPFDLNLPPPL-PPHDVDLSGDDLQATALCF 213
           RRVLPFDLNLPPPL PPHDVDLSGDDL ATALCF
Sbjct: 181 RRVLPFDLNLPPPLPPPHDVDLSGDDLHATALCF 214

[Close]

BLAST of Moc04g00870 vs. NCBI nr
Match: XP_023511720.1 (ethylene-responsive transcription factor 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.8 bits (961), Expect = 5.0e-100
Identity = 189/213 (88.73%), Postives = 194/213 (91.08%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGR  DP  A +T   P KEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRDMDPLMAAETVQEPDKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISNISVNRPTSSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH +IPFSQEFHDS GLCKIS+I VNRPTSSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQKIPFSQEFHDSAGLCKISDIPVNRPTSSS 120

Query: 121 LSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSIR 180
           LSSTVESFSGPRVSIPAPSVPK+ R+SLP VSPDDCHSDCDSSSSVVDDSEEYA TSSIR
Sbjct: 121 LSSTVESFSGPRVSIPAPSVPKICRQSLPLVSPDDCHSDCDSSSSVVDDSEEYAHTSSIR 180

Query: 181 RVLPFDLNLPPPLPP-HDVDLSGDDLQATALCF 213
           RVLPFDLNLPPPLPP HDVDLSGDDL ATALCF
Sbjct: 181 RVLPFDLNLPPPLPPLHDVDLSGDDLHATALCF 213

[Close]

BLAST of Moc04g00870 vs. NCBI nr
Match: XP_004140127.1 (ethylene-responsive transcription factor 3 [Cucumis sativus] >KGN47972.1 hypothetical protein Csa_003549 [Cucumis sativus])

HSP 1 Score: 373.6 bits (958), Expect = 1.1e-99
Identity = 189/214 (88.32%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGRATDP T  ++   PVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRATDPLTVPESVQKPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISN-ISVNRPTSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH QIPFSQ+FHDSGG C+ISN I VNRPTSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQQIPFSQQFHDSGGFCEISNIIPVNRPTSS 120

Query: 121 SLSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSI 180
           SLSSTVESFSGPR+SIPAPSVPKL RKSLP V+PDDCHSDCDSSSSVVDDSEEYA +SSI
Sbjct: 121 SLSSTVESFSGPRLSIPAPSVPKLRRKSLPQVAPDDCHSDCDSSSSVVDDSEEYAHSSSI 180

Query: 181 RRVLPFDLNLPPPL-PPHDVDLSGDDLQATALCF 213
           RRVLPFDLNLPPPL PPHDVDLSGDDL ATAL F
Sbjct: 181 RRVLPFDLNLPPPLPPPHDVDLSGDDLHATALYF 214

[Close]

BLAST of Moc04g00870 vs. NCBI nr
Match: XP_022953046.1 (ethylene-responsive transcription factor 3-like [Cucurbita moschata])

HSP 1 Score: 372.5 bits (955), Expect = 2.5e-99
Identity = 188/213 (88.26%), Postives = 193/213 (90.61%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGR  DP  A +T   P KEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRDMDPLMAAETVQEPDKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISNISVNRPTSSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH +IPFSQEFHDS GLCKIS+I VNRPTSSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQKIPFSQEFHDSAGLCKISDIPVNRPTSSS 120

Query: 121 LSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSIR 180
           LSSTVESFSGPRVSIPAP VPK+ R+SLP VSPDDCHSDCDSSSSVVDDSEEYA TSSIR
Sbjct: 121 LSSTVESFSGPRVSIPAPLVPKICRQSLPLVSPDDCHSDCDSSSSVVDDSEEYAHTSSIR 180

Query: 181 RVLPFDLNLPPPLPP-HDVDLSGDDLQATALCF 213
           RVLPFDLNLPPPLPP HDVDLSGDDL ATALCF
Sbjct: 181 RVLPFDLNLPPPLPPLHDVDLSGDDLHATALCF 213

[Close]

BLAST of Moc04g00870 vs. ExPASy Swiss-Prot
Match: Q9SXS8 (Ethylene-responsive transcription factor 3 OS=Nicotiana tabacum OX=4097 GN=ERF3 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.3e-55
Identity = 134/233 (57.51%), Postives = 152/233 (65.24%), Query Frame = 0


Query: 1   MRRGRATDPP---TAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTA 60
           MRRGRA   P   T      G  KE+RFRGVRKRPWGR+AAEIRDPWKKTRVWLGTFD+A
Sbjct: 1   MRRGRAAAAPAPVTGEPNGSGGSKEIRFRGVRKRPWGRFAAEIRDPWKKTRVWLGTFDSA 60

Query: 61  EDAARAYDAAALSLRGPKAKTNFPLHPSAISVHHQI-----PFSQEFHDSGGLCKISNIS 120
           EDAARAYDAAA +LRGPKAKTNFPL     + HHQ      P +  F DS    + + I 
Sbjct: 61  EDAARAYDAAARALRGPKAKTNFPL---PYAHHHQFNQGHNPNNDPFVDSRFYPQDNPII 120

Query: 121 VNRPTSSSLSSTVESFSGPRVSIPAP-----SVPKLHRKSLPPVSPDDCHSDCDSSSSVV 180
             RPTSSS+SSTVESFSGPR   PAP     +  + + +S PPV PDDCHSDCDSSSSVV
Sbjct: 121 SQRPTSSSMSSTVESFSGPRPP-PAPRQQTTASSRKYTRS-PPVVPDDCHSDCDSSSSVV 180

Query: 181 ---------DDSEEYARTSSIRRVLPFDLNLPPPLPPHDVDLSGDDLQATALC 212
                    D+  +   +SS R+ L FDLNLPPP+     D   DDL  TALC
Sbjct: 181 DHGDCEKENDNDNDNIASSSFRKPLLFDLNLPPPMD----DAGADDLHCTALC 224

[Close]

BLAST of Moc04g00870 vs. ExPASy Swiss-Prot
Match: O80339 (Ethylene-responsive transcription factor 3 OS=Arabidopsis thaliana OX=3702 GN=ERF3 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 3.4e-51
Identity = 127/230 (55.22%), Postives = 150/230 (65.22%), Query Frame = 0


Query: 1   MRRGR---ATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTA 60
           MRRGR   A   PT     +G VKE+RFRGVRKRPWGR+AAEIRDPWKK RVWLGTFD+A
Sbjct: 1   MRRGRGSSAVAGPTVVAAINGSVKEIRFRGVRKRPWGRFAAEIRDPWKKARVWLGTFDSA 60

Query: 61  EDAARAYDAAALSLRGPKAKTNFPLHPSA-------------ISVHHQIPFSQE--FHDS 120
           E+AARAYD+AA +LRGPKAKTNFP+  S+              + +   PF     F D 
Sbjct: 61  EEAARAYDSAARNLRGPKAKTNFPIDSSSPPPPNLRFNQIRNQNQNQVDPFMDHRLFTDH 120

Query: 121 GGLCKISNISVNRPTSSSLSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDS 180
                I    VNRPTSSS+SSTVESFSGPR +   P+  K + ++ PPV P+DCHSDCDS
Sbjct: 121 QQQFPI----VNRPTSSSMSSTVESFSGPRPTTMKPATTKRYPRT-PPVVPEDCHSDCDS 180

Query: 181 SSSVVDDSEEYARTSSIRRVLP--FDLNLPPPLPPHDVDLSGDDLQATAL 211
           SSSV+DD ++ A +SS RR  P  FDLN  PPL   D+    DDL  T L
Sbjct: 181 SSSVIDDDDDIA-SSSRRRNPPFQFDLNF-PPLDCVDLFNGADDLHCTDL 223

[Close]

BLAST of Moc04g00870 vs. ExPASy Swiss-Prot
Match: Q9LDE4 (Ethylene-responsive transcription factor 7 OS=Arabidopsis thaliana OX=3702 GN=ERF7 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.4e-44
Identity = 122/243 (50.21%), Postives = 147/243 (60.49%), Query Frame = 0


Query: 1   MRRGRATD--PPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAE 60
           MR+GR +    P    T  G VKE R+RGVRKRPWGR+AAEIRDP KK+RVWLGTFD+A 
Sbjct: 1   MRKGRGSSVVGPALPVTAGGSVKEPRYRGVRKRPWGRFAAEIRDPLKKSRVWLGTFDSAV 60

Query: 61  DAARAYDAAALSLRGPKAKTNFPLHPSAIS------------------VHHQI-PFSQEF 120
           DAARAYD AA +LRGPKAKTNFP+  S  S                  + +QI PF    
Sbjct: 61  DAARAYDTAARNLRGPKAKTNFPIDCSPSSPLQPLTYLHNQNLCSPPVIQNQIDPFMDHR 120

Query: 121 HDSGGLCKISNIS--VNRPTSSSLSSTVESFSGPR---VSIPAPSVPK-LHR----KSLP 180
              GG  +       ++RP SSS+SSTV+S SGPR    +  + SV K LH        P
Sbjct: 121 LYGGGNFQEQQQQQIISRPASSSMSSTVKSCSGPRPMEAAAASSSVAKPLHAIKRYPRTP 180

Query: 181 PVSPDDCHSDCDSSSSVVDDSEEYARTSSIRRV-LPFDLNLPPPLPPHDVDLSG-DDLQA 211
           PV+P+DCHSDCDSSSSV+DD ++ A +SS R+    FDLN  PPL   D+   G DDL  
Sbjct: 181 PVAPEDCHSDCDSSSSVIDDGDDIASSSSRRKTPFQFDLNF-PPLDGVDLFAGGIDDLHC 240

[Close]

BLAST of Moc04g00870 vs. ExPASy Swiss-Prot
Match: O80340 (Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 6.4e-26
Identity = 89/219 (40.64%), Postives = 107/219 (48.86%), Query Frame = 0


Query: 2   RRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDAA 61
           + G   DP T  Q TH   KE+R+RGVRKRPWGRYAAEIRDP KKTRVWLGTFDTAE+AA
Sbjct: 3   KMGLKPDPATTNQ-THNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA 62

Query: 62  RAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKIS--NISVNRPTSS 121
           RAYD AA   RG KAKTNFP             F++    S  L   S   + V   T+ 
Sbjct: 63  RAYDTAARDFRGAKAKTNFPTFLELSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAG 122

Query: 122 SLSSTVE--------------SFSGPRVSIPAPSVPKLHRKSLPPVSPD----------- 181
           ++   +E                S P   +    +  + R   PPV+             
Sbjct: 123 NVPPQLELSLGGGGGGSCYQIPMSRPVYFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATK 182

Query: 182 -DCHSDCDSSSSVVDDSEEYARTSSIRRVLPFDLNLPPP 193
             C +  DS SS V D E      S  ++L  DLNLPPP
Sbjct: 183 MACGAQSDSDSSSVVDFEGGMEKRS--QLLDLDLNLPPP 218

[Close]

BLAST of Moc04g00870 vs. ExPASy Swiss-Prot
Match: Q9C5I3 (Ethylene-responsive transcription factor 11 OS=Arabidopsis thaliana OX=3702 GN=ERF11 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.9e-25
Identity = 83/176 (47.16%), Postives = 100/176 (56.82%), Query Frame = 0


Query: 23  VRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDAARAYDAAALSLRGPKAKTNFPL 82
           VR+RGVRKRPWGRYAAEIRDP+KK+RVWLGTFDT E+AARAYD  A+  RG KAKTNFP 
Sbjct: 18  VRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARAYDKRAIEFRGAKAKTNFPC 77

Query: 83  HPSAISVHHQIPFSQEFHDSGGLCKISNISVNRPTSSSLSSTVESFSGPRVSIPAPSV-- 142
           +                        I+   ++   S S SSTVES S P +++ + SV  
Sbjct: 78  Y-----------------------NINAHCLSLTQSLSQSSTVES-SFPNLNLGSDSVSS 137

Query: 143 ----PKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSIRRVLPFDLNLPPP 193
               PK+  K+   V   D  S+ DSSS V+D      R    R VL  DLN PPP
Sbjct: 138 RFPFPKIQVKAGMMVF--DERSESDSSSVVMD----VVRYEGRRVVLDLDLNFPPP 163

[Close]

BLAST of Moc04g00870 vs. ExPASy TrEMBL
Match: A0A5D3C642 (Ethylene-responsive transcription factor 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00030 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 189/214 (88.32%), Postives = 197/214 (92.06%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGRATDP TA ++   PVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRATDPLTAPESVQEPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISN-ISVNRPTSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH QIPFSQ+FHDSGG C+ISN I VNRPTSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQQIPFSQQFHDSGGFCEISNIIPVNRPTSS 120

Query: 121 SLSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSI 180
           SLSSTVESFSGPR+SIP PSVPKL RKS P V+PDDCHSDCDSSSSVVDDSEEYA++SSI
Sbjct: 121 SLSSTVESFSGPRLSIPVPSVPKLRRKSQPQVAPDDCHSDCDSSSSVVDDSEEYAQSSSI 180

Query: 181 RRVLPFDLNLPPPL-PPHDVDLSGDDLQATALCF 213
           RRVLPFDLNLPPPL PPHDVDLSGDDL ATALCF
Sbjct: 181 RRVLPFDLNLPPPLPPPHDVDLSGDDLHATALCF 214

[Close]

BLAST of Moc04g00870 vs. ExPASy TrEMBL
Match: A0A1S3BLI0 (ethylene-responsive transcription factor 3-like OS=Cucumis melo OX=3656 GN=LOC103491361 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 189/214 (88.32%), Postives = 197/214 (92.06%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGRATDP TA ++   PVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRATDPLTAPESVQEPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISN-ISVNRPTSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH QIPFSQ+FHDSGG C+ISN I VNRPTSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQQIPFSQQFHDSGGFCEISNIIPVNRPTSS 120

Query: 121 SLSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSI 180
           SLSSTVESFSGPR+SIP PSVPKL RKS P V+PDDCHSDCDSSSSVVDDSEEYA++SSI
Sbjct: 121 SLSSTVESFSGPRLSIPVPSVPKLRRKSQPQVAPDDCHSDCDSSSSVVDDSEEYAQSSSI 180

Query: 181 RRVLPFDLNLPPPL-PPHDVDLSGDDLQATALCF 213
           RRVLPFDLNLPPPL PPHDVDLSGDDL ATALCF
Sbjct: 181 RRVLPFDLNLPPPLPPPHDVDLSGDDLHATALCF 214

[Close]

BLAST of Moc04g00870 vs. ExPASy TrEMBL
Match: A0A0A0KHW7 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421660 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.4e-100
Identity = 189/214 (88.32%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGRATDP T  ++   PVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRATDPLTVPESVQKPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISN-ISVNRPTSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH QIPFSQ+FHDSGG C+ISN I VNRPTSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQQIPFSQQFHDSGGFCEISNIIPVNRPTSS 120

Query: 121 SLSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSI 180
           SLSSTVESFSGPR+SIPAPSVPKL RKSLP V+PDDCHSDCDSSSSVVDDSEEYA +SSI
Sbjct: 121 SLSSTVESFSGPRLSIPAPSVPKLRRKSLPQVAPDDCHSDCDSSSSVVDDSEEYAHSSSI 180

Query: 181 RRVLPFDLNLPPPL-PPHDVDLSGDDLQATALCF 213
           RRVLPFDLNLPPPL PPHDVDLSGDDL ATAL F
Sbjct: 181 RRVLPFDLNLPPPLPPPHDVDLSGDDLHATALYF 214

[Close]

BLAST of Moc04g00870 vs. ExPASy TrEMBL
Match: A0A6J1GLX1 (ethylene-responsive transcription factor 3-like OS=Cucurbita moschata OX=3662 GN=LOC111455566 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.2e-99
Identity = 188/213 (88.26%), Postives = 193/213 (90.61%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGR  DP  A +T   P KEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRDMDPLMAAETVQEPDKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISNISVNRPTSSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH +IPFSQEFHDS GLCKIS+I VNRPTSSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQKIPFSQEFHDSAGLCKISDIPVNRPTSSS 120

Query: 121 LSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSIR 180
           LSSTVESFSGPRVSIPAP VPK+ R+SLP VSPDDCHSDCDSSSSVVDDSEEYA TSSIR
Sbjct: 121 LSSTVESFSGPRVSIPAPLVPKICRQSLPLVSPDDCHSDCDSSSSVVDDSEEYAHTSSIR 180

Query: 181 RVLPFDLNLPPPLPP-HDVDLSGDDLQATALCF 213
           RVLPFDLNLPPPLPP HDVDLSGDDL ATALCF
Sbjct: 181 RVLPFDLNLPPPLPPLHDVDLSGDDLHATALCF 213

[Close]

BLAST of Moc04g00870 vs. ExPASy TrEMBL
Match: A0A6J1HWB0 (ethylene-responsive transcription factor 3-like OS=Cucurbita maxima OX=3661 GN=LOC111468432 PE=4 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.0e-99
Identity = 188/213 (88.26%), Postives = 193/213 (90.61%), Query Frame = 0


Query: 1   MRRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60
           MRRGR  DP  A +T  GP KEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA
Sbjct: 1   MRRGRDMDPLMAAETVQGPGKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDA 60

Query: 61  ARAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKISNISVNRPTSSS 120
           ARAYD AALSLRGPKAKTNFPLHPS ISVH +IPFSQEFHDS GLCKIS+  VNRPTSSS
Sbjct: 61  ARAYDEAALSLRGPKAKTNFPLHPSTISVHQKIPFSQEFHDSTGLCKISDFPVNRPTSSS 120

Query: 121 LSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSIR 180
           LSSTVESFSGPRVSIPAPSVPK+ R+SLP VSPDDCHSDCDSSSSVVDDSEEYA TSSIR
Sbjct: 121 LSSTVESFSGPRVSIPAPSVPKICRQSLPLVSPDDCHSDCDSSSSVVDDSEEYAHTSSIR 180

Query: 181 RVLPFDLNLPPPLP-PHDVDLSGDDLQATALCF 213
           RVLPFDLNLPPPLP  HDVDLSGDDL ATALCF
Sbjct: 181 RVLPFDLNLPPPLPLLHDVDLSGDDLHATALCF 213

[Close]

BLAST of Moc04g00870 vs. TAIR 10
Match: AT1G50640.1 (ethylene responsive element binding factor 3 )

HSP 1 Score: 203.0 bits (515), Expect = 2.4e-52
Identity = 127/230 (55.22%), Postives = 150/230 (65.22%), Query Frame = 0


Query: 1   MRRGR---ATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTA 60
           MRRGR   A   PT     +G VKE+RFRGVRKRPWGR+AAEIRDPWKK RVWLGTFD+A
Sbjct: 1   MRRGRGSSAVAGPTVVAAINGSVKEIRFRGVRKRPWGRFAAEIRDPWKKARVWLGTFDSA 60

Query: 61  EDAARAYDAAALSLRGPKAKTNFPLHPSA-------------ISVHHQIPFSQE--FHDS 120
           E+AARAYD+AA +LRGPKAKTNFP+  S+              + +   PF     F D 
Sbjct: 61  EEAARAYDSAARNLRGPKAKTNFPIDSSSPPPPNLRFNQIRNQNQNQVDPFMDHRLFTDH 120

Query: 121 GGLCKISNISVNRPTSSSLSSTVESFSGPRVSIPAPSVPKLHRKSLPPVSPDDCHSDCDS 180
                I    VNRPTSSS+SSTVESFSGPR +   P+  K + ++ PPV P+DCHSDCDS
Sbjct: 121 QQQFPI----VNRPTSSSMSSTVESFSGPRPTTMKPATTKRYPRT-PPVVPEDCHSDCDS 180

Query: 181 SSSVVDDSEEYARTSSIRRVLP--FDLNLPPPLPPHDVDLSGDDLQATAL 211
           SSSV+DD ++ A +SS RR  P  FDLN  PPL   D+    DDL  T L
Sbjct: 181 SSSVIDDDDDIA-SSSRRRNPPFQFDLNF-PPLDCVDLFNGADDLHCTDL 223

[Close]

BLAST of Moc04g00870 vs. TAIR 10
Match: AT3G20310.1 (ethylene response factor 7 )

HSP 1 Score: 180.3 bits (456), Expect = 1.7e-45
Identity = 122/243 (50.21%), Postives = 147/243 (60.49%), Query Frame = 0


Query: 1   MRRGRATD--PPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAE 60
           MR+GR +    P    T  G VKE R+RGVRKRPWGR+AAEIRDP KK+RVWLGTFD+A 
Sbjct: 1   MRKGRGSSVVGPALPVTAGGSVKEPRYRGVRKRPWGRFAAEIRDPLKKSRVWLGTFDSAV 60

Query: 61  DAARAYDAAALSLRGPKAKTNFPLHPSAIS------------------VHHQI-PFSQEF 120
           DAARAYD AA +LRGPKAKTNFP+  S  S                  + +QI PF    
Sbjct: 61  DAARAYDTAARNLRGPKAKTNFPIDCSPSSPLQPLTYLHNQNLCSPPVIQNQIDPFMDHR 120

Query: 121 HDSGGLCKISNIS--VNRPTSSSLSSTVESFSGPR---VSIPAPSVPK-LHR----KSLP 180
              GG  +       ++RP SSS+SSTV+S SGPR    +  + SV K LH        P
Sbjct: 121 LYGGGNFQEQQQQQIISRPASSSMSSTVKSCSGPRPMEAAAASSSVAKPLHAIKRYPRTP 180

Query: 181 PVSPDDCHSDCDSSSSVVDDSEEYARTSSIRRV-LPFDLNLPPPLPPHDVDLSG-DDLQA 211
           PV+P+DCHSDCDSSSSV+DD ++ A +SS R+    FDLN  PPL   D+   G DDL  
Sbjct: 181 PVAPEDCHSDCDSSSSVIDDGDDIASSSSRRKTPFQFDLNF-PPLDGVDLFAGGIDDLHC 240

[Close]

BLAST of Moc04g00870 vs. TAIR 10
Match: AT3G15210.1 (ethylene responsive element binding factor 4 )

HSP 1 Score: 119.0 bits (297), Expect = 4.6e-27
Identity = 89/219 (40.64%), Postives = 107/219 (48.86%), Query Frame = 0


Query: 2   RRGRATDPPTAGQTTHGPVKEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDAA 61
           + G   DP T  Q TH   KE+R+RGVRKRPWGRYAAEIRDP KKTRVWLGTFDTAE+AA
Sbjct: 3   KMGLKPDPATTNQ-THNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA 62

Query: 62  RAYDAAALSLRGPKAKTNFPLHPSAISVHHQIPFSQEFHDSGGLCKIS--NISVNRPTSS 121
           RAYD AA   RG KAKTNFP             F++    S  L   S   + V   T+ 
Sbjct: 63  RAYDTAARDFRGAKAKTNFPTFLELSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAG 122

Query: 122 SLSSTVE--------------SFSGPRVSIPAPSVPKLHRKSLPPVSPD----------- 181
           ++   +E                S P   +    +  + R   PPV+             
Sbjct: 123 NVPPQLELSLGGGGGGSCYQIPMSRPVYFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATK 182

Query: 182 -DCHSDCDSSSSVVDDSEEYARTSSIRRVLPFDLNLPPP 193
             C +  DS SS V D E      S  ++L  DLNLPPP
Sbjct: 183 MACGAQSDSDSSSVVDFEGGMEKRS--QLLDLDLNLPPP 218

[Close]

BLAST of Moc04g00870 vs. TAIR 10
Match: AT1G28370.1 (ERF domain protein 11 )

HSP 1 Score: 117.5 bits (293), Expect = 1.3e-26
Identity = 83/176 (47.16%), Postives = 100/176 (56.82%), Query Frame = 0


Query: 23  VRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDAARAYDAAALSLRGPKAKTNFPL 82
           VR+RGVRKRPWGRYAAEIRDP+KK+RVWLGTFDT E+AARAYD  A+  RG KAKTNFP 
Sbjct: 18  VRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARAYDKRAIEFRGAKAKTNFPC 77

Query: 83  HPSAISVHHQIPFSQEFHDSGGLCKISNISVNRPTSSSLSSTVESFSGPRVSIPAPSV-- 142
           +                        I+   ++   S S SSTVES S P +++ + SV  
Sbjct: 78  Y-----------------------NINAHCLSLTQSLSQSSTVES-SFPNLNLGSDSVSS 137

Query: 143 ----PKLHRKSLPPVSPDDCHSDCDSSSSVVDDSEEYARTSSIRRVLPFDLNLPPP 193
               PK+  K+   V   D  S+ DSSS V+D      R    R VL  DLN PPP
Sbjct: 138 RFPFPKIQVKAGMMVF--DERSESDSSSVVMD----VVRYEGRRVVLDLDLNFPPP 163

[Close]

BLAST of Moc04g00870 vs. TAIR 10
Match: AT5G44210.1 (erf domain protein 9 )

HSP 1 Score: 114.8 bits (286), Expect = 8.6e-26
Identity = 55/70 (78.57%), Postives = 59/70 (84.29%), Query Frame = 0


Query: 21 KEVRFRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTAEDAARAYDAAALSLRGPKAKTNF 80
          KEV FRGVRKRPWGRYAAEIRDP KKTRVWLGTFDTAE+AARAYD AA   RG KAKTNF
Sbjct: 29 KEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAREFRGSKAKTNF 88

Query: 81 PLHPSAISVH 91
          PL   + +V+
Sbjct: 89 PLPGESTTVN 98

The following BLAST results are available for this feature:

BLAST of Moc04g00870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888524.1	9.7e-104	91.55	ethylene-responsive transcription factor 3-like [Benincasa hispida]	[more]
XP_008449487.1	2.2e-100	88.32	PREDICTED: ethylene-responsive transcription factor 3-like [Cucumis melo] >KAA00...	[more]
XP_023511720.1	5.0e-100	88.73	ethylene-responsive transcription factor 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_004140127.1	1.1e-99	88.32	ethylene-responsive transcription factor 3 [Cucumis sativus] >KGN47972.1 hypothe...	[more]
XP_022953046.1	2.5e-99	88.26	ethylene-responsive transcription factor 3-like [Cucurbita moschata]	[more]

BLAST of Moc04g00870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXS8	2.3e-55	57.51	Ethylene-responsive transcription factor 3 OS=Nicotiana tabacum OX=4097 GN=ERF3 ...	[more]
O80339	3.4e-51	55.22	Ethylene-responsive transcription factor 3 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]
Q9LDE4	2.4e-44	50.21	Ethylene-responsive transcription factor 7 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]
O80340	6.4e-26	40.64	Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]
Q9C5I3	1.9e-25	47.16	Ethylene-responsive transcription factor 11 OS=Arabidopsis thaliana OX=3702 GN=E...	[more]

BLAST of Moc04g00870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C642	1.1e-100	88.32	Ethylene-responsive transcription factor 3-like OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BLI0	1.1e-100	88.32	ethylene-responsive transcription factor 3-like OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0KHW7	5.4e-100	88.32	AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421660 PE=...	[more]
A0A6J1GLX1	1.2e-99	88.26	ethylene-responsive transcription factor 3-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1HWB0	2.0e-99	88.26	ethylene-responsive transcription factor 3-like OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Moc04g00870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G50640.1	2.4e-52	55.22	ethylene responsive element binding factor 3	[more]
AT3G20310.1	1.7e-45	50.21	ethylene response factor 7	[more]
AT3G15210.1	4.6e-27	40.64	ethylene responsive element binding factor 4	[more]
AT1G28370.1	1.3e-26	47.16	ERF domain protein 11	[more]
AT5G44210.1	8.6e-26	78.57	erf domain protein 9	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 25..36 score: 60.91 coord: 47..63 score: 73.9
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 24..87 e-value: 2.1E-37 score: 140.3
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 24..73 e-value: 7.7E-15 score: 54.9
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 24..81 score: 23.90708
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 24..81 e-value: 1.94029E-19 score: 76.5415
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 23..83 e-value: 8.0E-29 score: 101.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 139..172
None	No IPR available	PANTHER	PTHR31677	AP2 DOMAIN CLASS TRANSCRIPTION FACTOR	coord: 15..185
None	No IPR available	PANTHER	PTHR31677:SF157	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 3	coord: 15..185
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 25..82

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g00870.1	Moc04g00870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity