Homology
BLAST of Moc04g00170 vs. NCBI nr
Match:
XP_022147910.1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia])
HSP 1 Score: 1667.5 bits (4317), Expect = 0.0e+00
Identity = 822/822 (100.00%), Postives = 822/822 (100.00%), Query Frame = 0
Query: 1 MSGCFASTSSGSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTCSNSVSSASPFPGL 60
MSGCFASTSSGSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTCSNSVSSASPFPGL
Sbjct: 1 MSGCFASTSSGSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTCSNSVSSASPFPGL 60
Query: 61 DVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWV 120
DVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWV
Sbjct: 61 DVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWV 120
Query: 121 LDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATD 180
LDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATD
Sbjct: 121 LDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATD 180
Query: 181 EKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLA 240
EKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLA
Sbjct: 181 EKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLA 240
Query: 241 YVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVP 300
YVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVP
Sbjct: 241 YVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVP 300
Query: 301 TIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFR 360
TIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFR
Sbjct: 301 TIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFR 360
Query: 361 MLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSW 420
MLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSW
Sbjct: 361 MLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSW 420
Query: 421 TNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISK 480
TNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISK
Sbjct: 421 TNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISK 480
Query: 481 TTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFS 540
TTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFS
Sbjct: 481 TTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFS 540
Query: 541 AAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCS 600
AAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCS
Sbjct: 541 AAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCS 600
Query: 601 KEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMD 660
KEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMD
Sbjct: 601 KEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMD 660
Query: 661 EYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERE 720
EYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERE
Sbjct: 661 EYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERE 720
Query: 721 SSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLF 780
SSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLF
Sbjct: 721 SSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLF 780
Query: 781 WKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 823
WKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK
Sbjct: 781 WKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 822
BLAST of Moc04g00170 vs. NCBI nr
Match:
XP_022147908.1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia])
HSP 1 Score: 1655.2 bits (4285), Expect = 0.0e+00
Identity = 821/835 (98.32%), Postives = 821/835 (98.32%), Query Frame = 0
Query: 1 MSGCFASTSSG-------------STRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC 60
MSGCFASTSS STRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC
Sbjct: 1 MSGCFASTSSEYHLWRLPSMEAECSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC 60
Query: 61 SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS 120
SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS
Sbjct: 61 SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS 120
Query: 121 LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA 180
LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA
Sbjct: 121 LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA 180
Query: 181 LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRS 240
LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRS
Sbjct: 181 LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRS 240
Query: 241 CRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY 300
CRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY
Sbjct: 241 CRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY 300
Query: 301 LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED 360
LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED
Sbjct: 301 LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED 360
Query: 361 IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII 420
IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII
Sbjct: 361 IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII 420
Query: 421 YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA 480
YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA
Sbjct: 421 YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA 480
Query: 481 MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL 540
MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL
Sbjct: 481 MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL 540
Query: 541 KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV 600
KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV
Sbjct: 541 KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV 600
Query: 601 EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE 660
EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE
Sbjct: 601 EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE 660
Query: 661 AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC 720
AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC
Sbjct: 661 AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC 720
Query: 721 GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE 780
GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE
Sbjct: 721 GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE 780
Query: 781 KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 823
KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK
Sbjct: 781 KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 835
BLAST of Moc04g00170 vs. NCBI nr
Match:
XP_022147909.1 (ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia])
HSP 1 Score: 1645.2 bits (4259), Expect = 0.0e+00
Identity = 819/835 (98.08%), Postives = 819/835 (98.08%), Query Frame = 0
Query: 1 MSGCFASTSSG-------------STRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC 60
MSGCFASTSS STRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC
Sbjct: 1 MSGCFASTSSEYHLWRLPSMEAECSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC 60
Query: 61 SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS 120
SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS
Sbjct: 61 SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS 120
Query: 121 LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA 180
LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA
Sbjct: 121 LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA 180
Query: 181 LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRS 240
LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLR
Sbjct: 181 LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLR- 240
Query: 241 CRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY 300
RSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY
Sbjct: 241 -RSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY 300
Query: 301 LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED 360
LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED
Sbjct: 301 LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED 360
Query: 361 IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII 420
IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII
Sbjct: 361 IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII 420
Query: 421 YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA 480
YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA
Sbjct: 421 YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA 480
Query: 481 MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL 540
MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL
Sbjct: 481 MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL 540
Query: 541 KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV 600
KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV
Sbjct: 541 KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV 600
Query: 601 EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE 660
EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE
Sbjct: 601 EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE 660
Query: 661 AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC 720
AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC
Sbjct: 661 AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC 720
Query: 721 GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE 780
GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE
Sbjct: 721 GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE 780
Query: 781 KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 823
KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK
Sbjct: 781 KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 833
BLAST of Moc04g00170 vs. NCBI nr
Match:
XP_038888071.1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida] >XP_038888072.1 ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida])
HSP 1 Score: 1372.1 bits (3550), Expect = 0.0e+00
Identity = 676/804 (84.08%), Postives = 733/804 (91.17%), Query Frame = 0
Query: 19 SHEFPQRFASSRTATSTNMSLLRPANFTCSNSVSSASPFPGLDVETKTKTGALCFEETKE 78
SH+ P S + + M+L RP N C + SSAS FP LDV+TKTKTGAL EETKE
Sbjct: 38 SHDDPSSPISVHVQSISAMNLSRPTNVGCFTASSSASLFPELDVDTKTKTGALRLEETKE 97
Query: 79 RIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHL 138
RIKK+F+KVELSVSAYDTAWVAMVPSPNSLN PLFPECINWVLDNQHPDGSWGI+H+H L
Sbjct: 98 RIKKLFDKVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDNQHPDGSWGILHDHQL 157
Query: 139 LMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIE 198
LMKA+LLSTLAC LTLKRWNIG +HMSKAL+FIKSNI SATDEKQ SPVGFDIIFPGMIE
Sbjct: 158 LMKATLLSTLACVLTLKRWNIGQDHMSKALSFIKSNIASATDEKQRSPVGFDIIFPGMIE 217
Query: 199 YAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQY 258
YAKDL+LNLPLAP VDAL+QKKEL+LRSCRSNSEGGKAYLAYVSEGI L+DWEMVMQY
Sbjct: 218 YAKDLNLNLPLAPMKVDALVQKKELELRSCRSNSEGGKAYLAYVSEGIENLQDWEMVMQY 277
Query: 259 QRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQ 318
QRKNGSLFNSPSTTAVAFMH NDDGCFNYL S+LQKFD+SVPTIYPLDIYARL MVD+LQ
Sbjct: 278 QRKNGSLFNSPSTTAVAFMHRNDDGCFNYLRSVLQKFDDSVPTIYPLDIYARLHMVDSLQ 337
Query: 319 RLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFS 378
+ GI RHFKEEIRSVLDETYRCWMQGEE+IFLDASTCAMAFR+LR+ GYDVSSD+LTQFS
Sbjct: 338 KFGIGRHFKEEIRSVLDETYRCWMQGEENIFLDASTCAMAFRLLRVEGYDVSSDKLTQFS 397
Query: 379 EDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDR 438
E LF NCLGG+LKDFG+S+ELFKASQIIIYPDESILEN+NSWT+ FL+HGLS G V SDR
Sbjct: 398 EVLFPNCLGGHLKDFGASMELFKASQIIIYPDESILENVNSWTSRFLNHGLSGGLVRSDR 457
Query: 439 PDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAV 498
DRL+KQEA+NALEF Y+ TLERLLNKRA+E+Y+ DIVRISKTTY CLNFGHQDFLELAV
Sbjct: 458 TDRLVKQEAVNALEFPYNATLERLLNKRALESYSGDIVRISKTTYACLNFGHQDFLELAV 517
Query: 499 EDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWA 558
EDFN LQ IH KELKELE+WVVEN+LDKLKFARQKLAYCYFSAAATLTSPEL DARLSWA
Sbjct: 518 EDFNTLQCIHRKELKELEKWVVENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWA 577
Query: 559 KNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIE 618
KNGVLTTVVDDFFDVGGSE EL+NLIQLVEKWD NGE YCSKEVEIIFLALH TVC+I
Sbjct: 578 KNGVLTTVVDDFFDVGGSEEELLNLIQLVEKWDANGETGYCSKEVEIIFLALHSTVCDIG 637
Query: 619 KRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLP 678
+RAL WQGRSV RNVIDIWLALL+SMRKEAEWLKNK+ P MDEYMENGY+SFALGPIVLP
Sbjct: 638 RRALSWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSMDEYMENGYVSFALGPIVLP 697
Query: 679 ALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGG 738
L+FVGPKLPEEVIG+CEY KLFKLMSTCGRLLNDT++F+RESSEGKLNAMSLC+INAGG
Sbjct: 698 TLYFVGPKLPEEVIGNCEYKKLFKLMSTCGRLLNDTRTFDRESSEGKLNAMSLCIINAGG 757
Query: 739 DFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFT 798
TKEEA+EA+KG+VERTRRELLRLVLQE N+T+PRACKDLF KMSCVVHLFYRKDDGFT
Sbjct: 758 KLTKEEATEAMKGDVERTRRELLRLVLQE-NTTIPRACKDLFRKMSCVVHLFYRKDDGFT 817
Query: 799 SHELMNSAKALLEQPMVLDELLNK 823
SHELM SAKAL EQPMVLD+LLNK
Sbjct: 818 SHELMKSAKALFEQPMVLDQLLNK 840
BLAST of Moc04g00170 vs. NCBI nr
Match:
XP_038888074.1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 [Benincasa hispida])
HSP 1 Score: 1369.8 bits (3544), Expect = 0.0e+00
Identity = 672/786 (85.50%), Postives = 726/786 (92.37%), Query Frame = 0
Query: 37 MSLLRPANFTCSNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDT 96
M+L RP N C + SSAS FP LDV+TKTKTGAL EETKERIKK+F+KVELSVSAYDT
Sbjct: 1 MNLSRPTNVGCFTASSSASLFPELDVDTKTKTGALRLEETKERIKKLFDKVELSVSAYDT 60
Query: 97 AWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKR 156
AWVAMVPSPNSLN PLFPECINWVLDNQHPDGSWGI+H+H LLMKA+LLSTLAC LTLKR
Sbjct: 61 AWVAMVPSPNSLNKPLFPECINWVLDNQHPDGSWGILHDHQLLMKATLLSTLACVLTLKR 120
Query: 157 WNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDA 216
WNIG +HMSKAL+FIKSNI SATDEKQ SPVGFDIIFPGMIEYAKDL+LNLPLAP VDA
Sbjct: 121 WNIGQDHMSKALSFIKSNIASATDEKQRSPVGFDIIFPGMIEYAKDLNLNLPLAPMKVDA 180
Query: 217 LLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAF 276
L+QKKEL+LRSCRSNSEGGKAYLAYVSEGI L+DWEMVMQYQRKNGSLFNSPSTTAVAF
Sbjct: 181 LVQKKELELRSCRSNSEGGKAYLAYVSEGIENLQDWEMVMQYQRKNGSLFNSPSTTAVAF 240
Query: 277 MHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDE 336
MH NDDGCFNYL S+LQKFD+SVPTIYPLDIYARL MVD+LQ+ GI RHFKEEIRSVLDE
Sbjct: 241 MHRNDDGCFNYLRSVLQKFDDSVPTIYPLDIYARLHMVDSLQKFGIGRHFKEEIRSVLDE 300
Query: 337 TYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSS 396
TYRCWMQGEE+IFLDASTCAMAFR+LR+ GYDVSSD+LTQFSE LF NCLGG+LKDFG+S
Sbjct: 301 TYRCWMQGEENIFLDASTCAMAFRLLRVEGYDVSSDKLTQFSEVLFPNCLGGHLKDFGAS 360
Query: 397 LELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYS 456
+ELFKASQIIIYPDESILEN+NSWT+ FL+HGLS G V SDR DRL+KQEA+NALEF Y+
Sbjct: 361 MELFKASQIIIYPDESILENVNSWTSRFLNHGLSGGLVRSDRTDRLVKQEAVNALEFPYN 420
Query: 457 NTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELE 516
TLERLLNKRA+E+Y+ DIVRISKTTY CLNFGHQDFLELAVEDFN LQ IH KELKELE
Sbjct: 421 ATLERLLNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELE 480
Query: 517 RWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGS 576
+WVVEN+LDKLKFARQKLAYCYFSAAATLTSPEL DARLSWAKNGVLTTVVDDFFDVGGS
Sbjct: 481 KWVVENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGS 540
Query: 577 EGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDI 636
E EL+NLIQLVEKWD NGE YCSKEVEIIFLALH TVC+I +RAL WQGRSV RNVIDI
Sbjct: 541 EEELLNLIQLVEKWDANGETGYCSKEVEIIFLALHSTVCDIGRRALSWQGRSVTRNVIDI 600
Query: 637 WLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCE 696
WLALL+SMRKEAEWLKNK+ P MDEYMENGY+SFALGPIVLP L+FVGPKLPEEVIG+CE
Sbjct: 601 WLALLESMRKEAEWLKNKVVPSMDEYMENGYVSFALGPIVLPTLYFVGPKLPEEVIGNCE 660
Query: 697 YHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERT 756
Y KLFKLMSTCGRLLNDT++F+RESSEGKLNAMSLC+INAGG TKEEA+EA+KG+VERT
Sbjct: 661 YKKLFKLMSTCGRLLNDTRTFDRESSEGKLNAMSLCIINAGGKLTKEEATEAMKGDVERT 720
Query: 757 RRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVL 816
RRELLRLVLQE N+T+PRACKDLF KMSCVVHLFYRKDDGFTSHELM SAKAL EQPMVL
Sbjct: 721 RRELLRLVLQE-NTTIPRACKDLFRKMSCVVHLFYRKDDGFTSHELMKSAKALFEQPMVL 780
Query: 817 DELLNK 823
D+LLNK
Sbjct: 781 DQLLNK 785
BLAST of Moc04g00170 vs. ExPASy Swiss-Prot
Match:
Q39548 (Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1)
HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 619/790 (78.35%), Postives = 694/790 (87.85%), Query Frame = 0
Query: 37 MSLLRP---ANFTC-SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVS 96
M L RP A F S+S SSAS FPG+DV+T TKTGAL FEETKERIKK+F+KVELSVS
Sbjct: 1 MYLSRPTGVARFAASSSSSSSASLFPGVDVDTTTKTGALHFEETKERIKKLFDKVELSVS 60
Query: 97 AYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACAL 156
AYDTAWVAMVPSPNSLN PLFPECINWVLD+QH DGSWG++HN LLMKA+LLSTLAC L
Sbjct: 61 AYDTAWVAMVPSPNSLNQPLFPECINWVLDSQHADGSWGLLHNDQLLMKANLLSTLACVL 120
Query: 157 TLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPA 216
TLKRWNIGH+HMSKAL+FIKSNI SATDE Q SPVGFDIIFPGMIEYAKDL+LNLPLAP
Sbjct: 121 TLKRWNIGHDHMSKALDFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLAPT 180
Query: 217 NVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTT 276
NVDAL++KKEL+LRSCRSNSEGGKAYLAYVSEGIG+L+DW+MVMQYQRKNGSLFNSPSTT
Sbjct: 181 NVDALVRKKELELRSCRSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFNSPSTT 240
Query: 277 AVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRS 336
A AFMH NDDGCF+YL SLLQKFD SVPTIYPLDIYARL MVD+LQ+ GI RHFKEEIRS
Sbjct: 241 AAAFMHRNDDGCFDYLRSLLQKFDGSVPTIYPLDIYARLHMVDSLQKFGIARHFKEEIRS 300
Query: 337 VLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKD 396
VLDETYRCWMQGEE+IFLDASTCAMAFRMLR+ GYDVSSDQLTQFSED+F NCLGGYLKD
Sbjct: 301 VLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDIFPNCLGGYLKD 360
Query: 397 FGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALE 456
FG+SLEL+KASQII +PDES+LENINSWT+ FL HGLSS SV SDR D ++KQEA+NALE
Sbjct: 361 FGASLELYKASQIITHPDESVLENINSWTSRFLKHGLSSDSVWSDRTDSVVKQEAVNALE 420
Query: 457 FTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKEL 516
F Y+ TLERL++KRAME+Y+ DIVRISK+ Y CLNFGHQDFLELAVEDFN LQ IH KEL
Sbjct: 421 FPYNATLERLISKRAMESYSGDIVRISKSPYACLNFGHQDFLELAVEDFNTLQRIHLKEL 480
Query: 517 KELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFD 576
+EL+RWVVEN+LD+LKF R L YCYF+AAATLT PEL DAR++WA+NGVLTTVVDDF+D
Sbjct: 481 EELQRWVVENKLDELKFFRLHLGYCYFAAAATLTDPELHDARIAWAQNGVLTTVVDDFYD 540
Query: 577 VGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRN 636
GGSE EL NLI+LVEKWD +GE YCSK+VEI+FLALH TVCEI +RAL WQGRSVMRN
Sbjct: 541 GGGSEEELDNLIELVEKWDPDGEVGYCSKDVEIVFLALHSTVCEIGRRALVWQGRSVMRN 600
Query: 637 VIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVI 696
VID WLALLK MRKEAEW NK+ P M EYME ++SFALGPI+LP LFFVGPKL EE+I
Sbjct: 601 VIDGWLALLKVMRKEAEWSTNKVVPSMGEYMEQAHVSFALGPIILPMLFFVGPKLSEEMI 660
Query: 697 GSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGN 756
GSCEY KL+KLMST GRL ND +S++RE EGKLN +SL MI+ GG+ TKEEA EAIKG+
Sbjct: 661 GSCEYQKLYKLMSTAGRLKNDIRSYDRECKEGKLNILSLWMIDGGGNVTKEEAIEAIKGD 720
Query: 757 VERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQ 816
ER RELL LVLQE N+T+PRACKDLFWK+ +V+LFY +DDG+TS+ LMN+ KA+ EQ
Sbjct: 721 FERAIRELLGLVLQE-NTTIPRACKDLFWKLMSIVNLFYMEDDGYTSNRLMNTVKAMFEQ 780
Query: 817 PMVLDELLNK 823
PM LD LLNK
Sbjct: 781 PMDLDALLNK 789
BLAST of Moc04g00170 vs. ExPASy Swiss-Prot
Match:
A0A2K9RFY0 (Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 PE=1 SV=1)
HSP 1 Score: 885.6 bits (2287), Expect = 4.4e-256
Identity = 434/776 (55.93%), Postives = 575/776 (74.10%), Query Frame = 0
Query: 41 RPANFTCSNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVA 100
+ ++F C + V SAS G+ T K + CFEETKERI + +K ELSVS YDTAWVA
Sbjct: 14 KESHFRCFSHV-SASLDTGVRRVTSAKIASTCFEETKERIADLIHKAELSVSTYDTAWVA 73
Query: 101 MVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIG 160
MVPSPNS +P FP+C++W+L NQ DGSW H+H LL K L STLAC L LK+W +G
Sbjct: 74 MVPSPNSSQEPCFPDCLSWLLQNQCCDGSWACPHHHPLLKKDVLCSTLACVLALKKWGVG 133
Query: 161 HEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQK 220
E +++ ++FI+ N SA ++ Q SP+GFDIIFP M++YA+DL LNL L P ++ L+ K
Sbjct: 134 EEKINRGVHFIEHNFASAMEKCQISPMGFDIIFPAMLDYARDLLLNLRLEPTMLNDLIYK 193
Query: 221 KELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGN 280
+ L+L+ +++S +AYLAYV+EG+G+L+D VM++QR+NGSLFNSPSTTA AF+
Sbjct: 194 RGLELKRNQNHSAEREAYLAYVAEGMGKLQDLGSVMKHQRRNGSLFNSPSTTAAAFIAFP 253
Query: 281 DDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRC 340
+ C YL S L+KF ++VP +YPLDIY +LC VDNL+RLGI R+F++EI+ VLDETYRC
Sbjct: 254 NSRCLTYLRSALKKFGSAVPAVYPLDIYLQLCTVDNLERLGISRYFQKEIQGVLDETYRC 313
Query: 341 WMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELF 400
W+QG E+IF+DA TCA+AFR+LR GY+V+SD +T+ E+ FS+ G +KD ++L L+
Sbjct: 314 WLQGNEEIFMDAPTCALAFRVLRKNGYNVTSDPITKLLEECFSSSFCGNIKDINTTLGLY 373
Query: 401 KASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLE 460
+AS+ I+YPDE LE N + L LSS +HS + R + E +ALE+ + L+
Sbjct: 374 RASEFILYPDERDLEKQNLMLKNLLEQELSSDFIHSSQLGRNIDAEVKHALEYPFYADLD 433
Query: 461 RLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVV 520
R++N+R +E+YN D RI KT+Y NFG++DFL L+V+D+N Q+IH +EL+ELERWV+
Sbjct: 434 RIVNRRNIEHYNFDNTRILKTSYCSPNFGNKDFLFLSVKDYNECQAIHREELRELERWVI 493
Query: 521 ENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGEL 580
ENRLD+L+FARQK AYCYFSAAATL +PEL +AR+SWAKNGVLTTVVDDFFD+GGS EL
Sbjct: 494 ENRLDELRFARQKCAYCYFSAAATLFAPELSNARMSWAKNGVLTTVVDDFFDLGGSVEEL 553
Query: 581 INLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLAL 640
NLIQLVE WDV+ + S+ V+IIF AL T+C+I + Q RS+ ++IDIWL L
Sbjct: 554 KNLIQLVELWDVDVSTECSSQNVQIIFSALKCTICDIGDKGSKLQERSITNHIIDIWLDL 613
Query: 641 LKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKL 700
L SM KE EW ++K P MDEY+ N Y+SFALGPIVLPAL+ VGPKL EE++ EYH L
Sbjct: 614 LYSMMKETEWARDKYIPTMDEYISNAYVSFALGPIVLPALYLVGPKLSEEMVHHSEYHNL 673
Query: 701 FKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRREL 760
+KLMSTCGRLLND + ERE EGKLNA+ L +IN GG+ TKE A+ +K +E RREL
Sbjct: 674 YKLMSTCGRLLNDIRGCERELKEGKLNAIPLYIINNGGEITKEAAASEMKSLIETHRREL 733
Query: 761 LRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVL 817
LRLVL+ KNS +P++CK+LFW MS V+HLFY KDDGFTS +L+ KA++ +P+VL
Sbjct: 734 LRLVLEGKNSVLPKSCKELFWHMSKVLHLFYSKDDGFTSQDLIKVVKAVIYEPIVL 788
BLAST of Moc04g00170 vs. ExPASy Swiss-Prot
Match:
A0A1Z3GC64 (Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1 SV=1)
HSP 1 Score: 880.2 bits (2273), Expect = 1.9e-254
Identity = 436/788 (55.33%), Postives = 572/788 (72.59%), Query Frame = 0
Query: 35 TNMSLLRPANFTCSNSVSSASPFPGLDVETKTKTG--ALCFEETKERIKKMFNKVELSVS 94
+N +L+ P + S +SAS GL T + A CFEETK RI K+F+K ELSVS
Sbjct: 6 SNSALVGPKFRSSRISHASASLDIGLQRATSPQNASVATCFEETKGRIAKLFHKNELSVS 65
Query: 95 AYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACAL 154
YDTAWVAMVPSP S +P FP C+NW+L+NQ DGSW H+HH+L K L STLAC L
Sbjct: 66 TYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCHDGSWARPHHHHMLKKDVLSSTLACIL 125
Query: 155 TLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPA 214
LK+W +G E +++ L+F++ N SAT++ Q +P+GFDIIFP M++ A+ L LNL L P
Sbjct: 126 ALKKWGVGEEQINRGLHFVELNFASATEKGQITPMGFDIIFPAMLDNARGLSLNLQLEPT 185
Query: 215 NVDALLQKKELDLRSC-RSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPST 274
++ L+ K++L+L+ C +SNS + Y A+++EG+G+L+DWE VM+YQRKNGSLFN PST
Sbjct: 186 TLNDLIYKRDLELKRCNQSNSAEKEVYWAHIAEGMGKLQDWESVMKYQRKNGSLFNCPST 245
Query: 275 TAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIR 334
TA AF + C NYL L+KF ++VP +YPLDIY++LC VDNL+RLGI R+F EI+
Sbjct: 246 TAAAFTALRNSDCLNYLRLALEKFGSAVPAVYPLDIYSQLCTVDNLERLGISRYFLTEIQ 305
Query: 335 SVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLK 394
SVLDETYR W+QG+E+IF+DASTCA+AFR LR+ GY+V+SD +T+ ++ FS+ G +
Sbjct: 306 SVLDETYRSWLQGDEEIFMDASTCALAFRTLRMNGYNVTSDPITKILQECFSSSFRGNMT 365
Query: 395 DFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSS-GSVHSDRPDRLLKQEAINA 454
D ++LE+++AS++I+YP+E L+ N +FL LSS G + S + R + E A
Sbjct: 366 DINTTLEIYRASELILYPEERDLDQHNLRLKTFLEQELSSNGFIQSCQLGRNINAEVNQA 425
Query: 455 LEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHK 514
+E+ + ++R+ ++ +ENYNID RI KT+Y NFG++DFL L+VEDFN Q IH +
Sbjct: 426 IEYPFYAIMDRMAKRKNIENYNIDNTRILKTSYRSPNFGNKDFLSLSVEDFNRCQVIHRE 485
Query: 515 ELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDF 574
EL+ELERWV+ENRLD+LKFAR K AYCYFSAAAT+ SPEL DAR+SWAKNGVLTTVVDDF
Sbjct: 486 ELRELERWVIENRLDELKFARSKAAYCYFSAAATIFSPELSDARMSWAKNGVLTTVVDDF 545
Query: 575 FDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVM 634
FDVGGS EL NLIQLVE WDV+ CS V+IIF AL T+CEI + QGRS+
Sbjct: 546 FDVGGSVEELKNLIQLVELWDVDVSTQCCSPNVQIIFSALKHTICEIADKGFKLQGRSIT 605
Query: 635 RNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEE 694
++I IWL LL SM KE E +K P MDEYM N Y+SFALGPIVLPAL+ VGPKL EE
Sbjct: 606 DHIISIWLDLLYSMMKETELGIDKSFPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEE 665
Query: 695 VIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIK 754
++ EYH LFKLMSTCGRLLND + +ERE +GK++A+SL ++N GG+ T E A ++
Sbjct: 666 MVNHSEYHTLFKLMSTCGRLLNDIRGYERELKDGKISAVSLYIMNNGGEITTEAAISEMR 725
Query: 755 GNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALL 814
+ER RRELLRLVL+E S +P+ACK LFW M VVHLFYRKDDGFTS +L A++
Sbjct: 726 SWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHLFYRKDDGFTSLDLHGVVNAII 785
Query: 815 EQPMVLDE 819
+P+VL++
Sbjct: 786 NEPIVLNQ 793
BLAST of Moc04g00170 vs. ExPASy Swiss-Prot
Match:
A0A0U4CDK4 (Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS PE=1 SV=1)
HSP 1 Score: 871.3 bits (2250), Expect = 8.6e-252
Identity = 425/772 (55.05%), Postives = 557/772 (72.15%), Query Frame = 0
Query: 50 SVSSASPFPGLDVETKTKTGAL--CFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS 109
S +SAS GL T K ++ CFE+T+ RI K+F+K ELSVS YDTAWVAMVPSP S
Sbjct: 24 SPASASLKSGLHSATSAKIASMPTCFEQTRGRIAKLFHKDELSVSTYDTAWVAMVPSPTS 83
Query: 110 LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA 169
L +P FP+C+NW+L+NQ DGSW H+H LL K L STLAC L LK+W +G E + +
Sbjct: 84 LEEPCFPDCLNWLLENQCHDGSWARPHHHPLLKKDVLSSTLACILALKKWGVGEEQIKRG 143
Query: 170 LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDL-R 229
L+F++ N SATD+ Q +P+GFDIIFP M++YA+ LNL L P + L+ K++++L R
Sbjct: 144 LHFLELNFASATDKCQITPMGFDIIFPAMLDYARGFSLNLRLDPTTFNDLMHKRDVELKR 203
Query: 230 SCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFN 289
S R+ S + Y AY++EG+G L++WE VM+YQR+NGSLFN PSTTA AF+ + C N
Sbjct: 204 SNRNYSSETETYWAYIAEGMGELQNWESVMKYQRRNGSLFNCPSTTAAAFIALRNSDCLN 263
Query: 290 YLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEE 349
YLH L+KF N+V +YPLDIY++LC VDNL+RLGI ++F EI++VLDETYRCWMQG E
Sbjct: 264 YLHLALKKFGNAVSAVYPLDIYSQLCTVDNLERLGISQYFSTEIQNVLDETYRCWMQGNE 323
Query: 350 DIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQII 409
+IF+DASTCA+AFR LRL GYDV+SD +TQ ++ FS+ G + D ++LEL++AS+++
Sbjct: 324 EIFMDASTCALAFRTLRLNGYDVTSDPVTQILQECFSSSFRGNMTDINTTLELYRASELV 383
Query: 410 IYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKR 469
+YPDE LE N L LSSG + S + R + E A+E+ + ++R+ ++
Sbjct: 384 LYPDERDLEKQNLRLKLLLEQELSSGLIQSCQLGRSINVEVNQAIEYPFYAIMDRVAKRK 443
Query: 470 AMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDK 529
++E+YN D RI KT+Y NF ++DFL L++EDFN Q+ H +EL ELERWVVENRLD+
Sbjct: 444 SIEHYNFDNTRILKTSYCSPNFSNEDFLFLSIEDFNRCQAAHREELGELERWVVENRLDE 503
Query: 530 LKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQL 589
LKFAR K AYCYFSAAAT +PEL DARLSWAKNGVLTTV+DDFFDVGGS EL NLIQL
Sbjct: 504 LKFARSKSAYCYFSAAATFFAPELLDARLSWAKNGVLTTVIDDFFDVGGSVEELKNLIQL 563
Query: 590 VEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRK 649
VE WDV+ + S V+IIF AL T+CEI +A QGR + ++I IWL LL SM +
Sbjct: 564 VELWDVDISTECYSHNVQIIFSALRRTICEIGDKAFKLQGRCITNHIIAIWLDLLNSMMR 623
Query: 650 EAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMST 709
E EW ++ P +DEYM N ++SFALGPIVLPAL+ VGPKL EE++ EYH LFKLMST
Sbjct: 624 ETEWARDNFVPTIDEYMSNAHVSFALGPIVLPALYLVGPKLSEEMVNHSEYHNLFKLMST 683
Query: 710 CGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQ 769
CGRLLND +ERE +GKLNA+SL +IN GG+ +KE A +K +E RRELLRLVL+
Sbjct: 684 CGRLLNDIHGYERELKDGKLNALSLYIINHGGEVSKEAAIWEMKSWIETQRRELLRLVLE 743
Query: 770 EKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDE 819
K S +P+ C++LFW M VVHLFY K DGFTS +L+ ++ QP++L++
Sbjct: 744 GKKSVLPKPCRELFWHMCSVVHLFYSKGDGFTSQDLIQLVNTIIHQPILLND 795
BLAST of Moc04g00170 vs. ExPASy Swiss-Prot
Match:
A0A3G1QTS7 (Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 SV=1)
HSP 1 Score: 866.7 bits (2238), Expect = 2.1e-250
Identity = 432/788 (54.82%), Postives = 567/788 (71.95%), Query Frame = 0
Query: 35 TNMSLLRPANFTCSNSVSSASPFPGLDVETKTKTGAL--CFEETKERIKKMFNKVELSVS 94
+N L+ P + S +SAS GL T + ++ CFEETK RI K+F+K ELSVS
Sbjct: 6 SNSVLVGPKFRSSRISHASASLDIGLQRATSPQNASVPTCFEETKGRIAKLFHKNELSVS 65
Query: 95 AYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACAL 154
YDTAWVAMVPSP S +P FP C+NW+L+NQ DGSW H+HH+L K L STLAC L
Sbjct: 66 TYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCHDGSWARPHHHHMLKKDVLSSTLACIL 125
Query: 155 TLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPA 214
LK+W +G E +++ L+F++ N SAT++ Q +P+GFDIIFP M++ A+ L LNL L P
Sbjct: 126 ALKKWGVGEEQINRGLHFVELNFASATEKGQITPMGFDIIFPAMLDNARGLSLNLQLEPT 185
Query: 215 NVDALLQKKELDLRSC-RSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPST 274
++ L+ K++L+L+ C +SNS + Y A+++EG+G+L DWE VM+YQRKNGSLFN PST
Sbjct: 186 MLNDLIYKRDLELKRCNQSNSAEKEVYWAHIAEGMGKLHDWESVMKYQRKNGSLFNCPST 245
Query: 275 TAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIR 334
TA AF + C NYL L+KF ++VP +YPLDIY++LC V NL+RLGI R+F EI+
Sbjct: 246 TAAAFTALRNSDCLNYLCLALEKFGSAVPAVYPLDIYSQLCTVGNLERLGISRYFLTEIQ 305
Query: 335 SVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLK 394
SVLDETYR W+QG+E+IF+DASTCA+AFR LR+ GY+V+SD +T+ ++ FS+ G +
Sbjct: 306 SVLDETYRSWLQGDEEIFMDASTCALAFRTLRMNGYNVTSDPITKILQECFSSSFRGNMT 365
Query: 395 DFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSS-GSVHSDRPDRLLKQEAINA 454
D ++LE+++AS++I+YP+E L N +FL LSS G + S + R + E A
Sbjct: 366 DNNTTLEIYRASELILYPEERDLVQHNLRLKTFLEQELSSNGFIQSCQLGRNINAEVNQA 425
Query: 455 LEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHK 514
+E+ + ++R+ ++ +ENYNID RI KT+Y NFG++DFL L+VEDFN Q IH +
Sbjct: 426 IEYPFYAIMDRMAKRKNIENYNIDNTRILKTSYRSPNFGNKDFLSLSVEDFNRCQVIHRE 485
Query: 515 ELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDF 574
EL+ELERWV+ENRLD+LKFAR K AYCYFSAAAT+ SPEL DAR+SWAKNGVLTTVVDDF
Sbjct: 486 ELRELERWVIENRLDELKFARSKAAYCYFSAAATIFSPELSDARMSWAKNGVLTTVVDDF 545
Query: 575 FDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVM 634
FDVGGS EL NLIQLVE WDV+ CS V+IIF AL T+CEI + QGRS+
Sbjct: 546 FDVGGSVEELKNLIQLVELWDVDVSTQCCSPNVQIIFSALKHTICEIADKGFKLQGRSIT 605
Query: 635 RNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEE 694
++I IWL LL SM KE E +K P MDEYM N Y+SFALGPIVLPAL+ VGPKL EE
Sbjct: 606 DHIISIWLDLLYSMMKETELGIDKSFPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEE 665
Query: 695 VIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIK 754
++ EYH LFKLMSTCGRLLND + +ERE +GK++A+SL ++N GG+ T E A ++
Sbjct: 666 MVNHSEYHTLFKLMSTCGRLLNDIRGYERELKDGKISAVSLYIMNNGGEITTEAAISEMR 725
Query: 755 GNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALL 814
+ER RRELLRLVL+E S +P+ACK LFW M VVH+FY KDDGFTS L A++
Sbjct: 726 SWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHVFYSKDDGFTSLNLHGVVNAII 785
Query: 815 EQPMVLDE 819
+P+VL++
Sbjct: 786 NEPIVLNQ 793
BLAST of Moc04g00170 vs. ExPASy TrEMBL
Match:
A0A6J1D1G1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)
HSP 1 Score: 1667.5 bits (4317), Expect = 0.0e+00
Identity = 822/822 (100.00%), Postives = 822/822 (100.00%), Query Frame = 0
Query: 1 MSGCFASTSSGSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTCSNSVSSASPFPGL 60
MSGCFASTSSGSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTCSNSVSSASPFPGL
Sbjct: 1 MSGCFASTSSGSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTCSNSVSSASPFPGL 60
Query: 61 DVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWV 120
DVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWV
Sbjct: 61 DVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWV 120
Query: 121 LDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATD 180
LDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATD
Sbjct: 121 LDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATD 180
Query: 181 EKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLA 240
EKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLA
Sbjct: 181 EKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLA 240
Query: 241 YVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVP 300
YVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVP
Sbjct: 241 YVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVP 300
Query: 301 TIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFR 360
TIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFR
Sbjct: 301 TIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFR 360
Query: 361 MLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSW 420
MLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSW
Sbjct: 361 MLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSW 420
Query: 421 TNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISK 480
TNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISK
Sbjct: 421 TNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISK 480
Query: 481 TTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFS 540
TTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFS
Sbjct: 481 TTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFS 540
Query: 541 AAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCS 600
AAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCS
Sbjct: 541 AAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCS 600
Query: 601 KEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMD 660
KEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMD
Sbjct: 601 KEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMD 660
Query: 661 EYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERE 720
EYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERE
Sbjct: 661 EYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERE 720
Query: 721 SSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLF 780
SSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLF
Sbjct: 721 SSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLF 780
Query: 781 WKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 823
WKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK
Sbjct: 781 WKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 822
BLAST of Moc04g00170 vs. ExPASy TrEMBL
Match:
A0A6J1D2L1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)
HSP 1 Score: 1655.2 bits (4285), Expect = 0.0e+00
Identity = 821/835 (98.32%), Postives = 821/835 (98.32%), Query Frame = 0
Query: 1 MSGCFASTSSG-------------STRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC 60
MSGCFASTSS STRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC
Sbjct: 1 MSGCFASTSSEYHLWRLPSMEAECSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC 60
Query: 61 SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS 120
SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS
Sbjct: 61 SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS 120
Query: 121 LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA 180
LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA
Sbjct: 121 LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA 180
Query: 181 LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRS 240
LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRS
Sbjct: 181 LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRS 240
Query: 241 CRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY 300
CRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY
Sbjct: 241 CRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY 300
Query: 301 LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED 360
LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED
Sbjct: 301 LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED 360
Query: 361 IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII 420
IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII
Sbjct: 361 IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII 420
Query: 421 YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA 480
YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA
Sbjct: 421 YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA 480
Query: 481 MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL 540
MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL
Sbjct: 481 MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL 540
Query: 541 KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV 600
KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV
Sbjct: 541 KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV 600
Query: 601 EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE 660
EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE
Sbjct: 601 EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE 660
Query: 661 AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC 720
AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC
Sbjct: 661 AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC 720
Query: 721 GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE 780
GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE
Sbjct: 721 GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE 780
Query: 781 KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 823
KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK
Sbjct: 781 KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 835
BLAST of Moc04g00170 vs. ExPASy TrEMBL
Match:
A0A6J1D2F0 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)
HSP 1 Score: 1645.2 bits (4259), Expect = 0.0e+00
Identity = 819/835 (98.08%), Postives = 819/835 (98.08%), Query Frame = 0
Query: 1 MSGCFASTSSG-------------STRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC 60
MSGCFASTSS STRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC
Sbjct: 1 MSGCFASTSSEYHLWRLPSMEAECSTRLQYCSHEFPQRFASSRTATSTNMSLLRPANFTC 60
Query: 61 SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS 120
SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS
Sbjct: 61 SNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNS 120
Query: 121 LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA 180
LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA
Sbjct: 121 LNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKA 180
Query: 181 LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRS 240
LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLR
Sbjct: 181 LNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLR- 240
Query: 241 CRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY 300
RSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY
Sbjct: 241 -RSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNY 300
Query: 301 LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED 360
LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED
Sbjct: 301 LHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED 360
Query: 361 IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII 420
IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII
Sbjct: 361 IFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIII 420
Query: 421 YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA 480
YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA
Sbjct: 421 YPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRA 480
Query: 481 MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL 540
MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL
Sbjct: 481 MENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKL 540
Query: 541 KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV 600
KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV
Sbjct: 541 KFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLV 600
Query: 601 EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE 660
EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE
Sbjct: 601 EKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKE 660
Query: 661 AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC 720
AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC
Sbjct: 661 AEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTC 720
Query: 721 GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE 780
GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE
Sbjct: 721 GRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQE 780
Query: 781 KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 823
KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK
Sbjct: 781 KNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDELLNK 833
BLAST of Moc04g00170 vs. ExPASy TrEMBL
Match:
A0A1S3BG06 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)
HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 662/786 (84.22%), Postives = 728/786 (92.62%), Query Frame = 0
Query: 37 MSLLRPANFTCSNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDT 96
M+L RP N C SSAS FPGLDV+TKTKTGAL FE+TKERI+K+F VELSVSAYDT
Sbjct: 1 MNLSRPTNLGCFTPSSSASLFPGLDVDTKTKTGALRFEDTKERIQKLFKNVELSVSAYDT 60
Query: 97 AWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKR 156
AWVAMVPSPNSLN PLFPECINWVLD+Q+PDGSWGI+H+H L+MKA+LLSTLAC LTLKR
Sbjct: 61 AWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKR 120
Query: 157 WNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDA 216
WNIGH+HMSKAL+FIKSNI SATDE Q SPVGFDIIFPGMIEYAKDL+LNLPLA NVDA
Sbjct: 121 WNIGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDA 180
Query: 217 LLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAF 276
L+QKKEL+LRSC SNSEGGKAYLAYVSEGIG+L+DW+MVMQYQRKNGSLF+SPSTTAVAF
Sbjct: 181 LVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAF 240
Query: 277 MHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDE 336
MH NDDGCFNYL S+LQKF +SVP IYPLDIYARL MVD+LQ+LGIDRHFK+EIRSVLDE
Sbjct: 241 MHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDE 300
Query: 337 TYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSS 396
TYRCWMQGEE+IFLDASTCAMAFRMLR+ GYDVSSDQLTQFSEDLF NCLGG+LKDFG+S
Sbjct: 301 TYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGAS 360
Query: 397 LELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYS 456
LELFKASQIIIYPDE ILENINSWT+ FL+HGLS GSVHSDR +RL+KQEA+NA EF Y+
Sbjct: 361 LELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYN 420
Query: 457 NTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELE 516
+TLERL NKRA+E+Y+ DIVRISKTTY CLNFGHQDFLELAVEDFN LQ IH KELKELE
Sbjct: 421 STLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELE 480
Query: 517 RWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGS 576
+WV+EN+LDKLKFARQKLAYCYFSAAATLTSPEL DARLSWAKNGVLTTVVDDFFDVGGS
Sbjct: 481 KWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGS 540
Query: 577 EGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDI 636
E EL+NLIQLVEKWD +GEA YCSKEVEIIFLALH T+CEI ++ALPWQGRSV RNVIDI
Sbjct: 541 EEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDI 600
Query: 637 WLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCE 696
WLALL+SMRKEAEWLKNK+ P +DEYMENGY+SFALGPIVLP L+FVGPKLPEE+IG+CE
Sbjct: 601 WLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCE 660
Query: 697 YHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERT 756
Y KLFKLMST GRLLNDT++F+RESSEGKLNA+SL +I+AGG +KEEA+EA+KG+V+RT
Sbjct: 661 YQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSKEEATEAMKGDVDRT 720
Query: 757 RRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVL 816
RRELLRLVLQE NST+PRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKAL E PMVL
Sbjct: 721 RRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVL 780
Query: 817 DELLNK 823
DELLNK
Sbjct: 781 DELLNK 785
BLAST of Moc04g00170 vs. ExPASy TrEMBL
Match:
Q9FRX5 (Ent-kaurene synthase OS=Cucumis sativus OX=3659 GN=CsKS1 PE=2 SV=1)
HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 661/786 (84.10%), Postives = 725/786 (92.24%), Query Frame = 0
Query: 37 MSLLRPANFTCSNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDT 96
M+L RP N C + SSAS FPGLDV TKTKTGAL FEETKERIKK+F VELS+SAYDT
Sbjct: 1 MNLSRPTNLGCFTASSSASLFPGLDVGTKTKTGALRFEETKERIKKLFKNVELSISAYDT 60
Query: 97 AWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKASLLSTLACALTLKR 156
AWVAMVPSPNSLN PLFPECINWVLD+Q+PDGSWGI+H+H L+MKA+LLSTLAC LTLKR
Sbjct: 61 AWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKR 120
Query: 157 WNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDA 216
W+IG +HMSKAL+FIKSNI SATDE Q SPVGFDIIFPGMIEYAKDL+LNLPLA NVDA
Sbjct: 121 WDIGDDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDA 180
Query: 217 LLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAF 276
L+QKKEL+LRSC SNSEGGKAYLAYVSEGIG+L+DWEMVM+YQRKNGSLF+SPSTTAVAF
Sbjct: 181 LVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWEMVMRYQRKNGSLFSSPSTTAVAF 240
Query: 277 MHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDE 336
MH NDDGCFNYL S+LQKF +SVP IYPLDIYARL MVD+LQ+LGID HFK+EIRSVLDE
Sbjct: 241 MHRNDDGCFNYLRSVLQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDGHFKDEIRSVLDE 300
Query: 337 TYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSS 396
TY CWMQGEE+IFLDASTCAMAFRMLR+ GYDVSSDQLTQFSE LFSNCLGG+LKDF +S
Sbjct: 301 TYSCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEGLFSNCLGGHLKDFSAS 360
Query: 397 LELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYS 456
LELFKASQIIIYPDE ILENINSWT+ FL+HGLSSGSVHSDR +RL+KQEA+NA EF Y+
Sbjct: 361 LELFKASQIIIYPDEFILENINSWTSRFLNHGLSSGSVHSDRTERLVKQEAVNAFEFPYN 420
Query: 457 NTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELE 516
+TLERL NKRA+E+Y+ DIVRISKT Y CLNFGHQDFLELAVEDFN LQ IH KELKELE
Sbjct: 421 STLERLSNKRALESYSGDIVRISKTAYACLNFGHQDFLELAVEDFNTLQGIHRKELKELE 480
Query: 517 RWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGS 576
+WV+EN+LDKLKFARQKLAYCYFSAAATLTSPEL DARLSWAKNGVLTTVVDDFFDVGGS
Sbjct: 481 KWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGS 540
Query: 577 EGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDI 636
E EL+NLIQLVEKWD +GE YCSKEVEIIFLALH T+CEI K+ALPWQGRSVMRNVIDI
Sbjct: 541 EEELVNLIQLVEKWDASGETGYCSKEVEIIFLALHSTICEIGKKALPWQGRSVMRNVIDI 600
Query: 637 WLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCE 696
WLALL+SMRKEAEWLKNK+ P +DEYMENGY+SFALGPIVLP L+FVGPKLPEE++G+CE
Sbjct: 601 WLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIVGNCE 660
Query: 697 YHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERT 756
Y KLFKLMST GRLLNDT++F+RESSEGKLNA+SL MI+AGG TKEEA+EA+KG+V+RT
Sbjct: 661 YKKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYMISAGGKLTKEEATEAMKGDVDRT 720
Query: 757 RRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVL 816
RRELLRLVLQE NST+PRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKAL EQPMVL
Sbjct: 721 RRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFEQPMVL 780
Query: 817 DELLNK 823
DELLNK
Sbjct: 781 DELLNK 785
BLAST of Moc04g00170 vs. TAIR 10
Match:
AT1G79460.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 840.1 bits (2169), Expect = 1.5e-243
Identity = 434/777 (55.86%), Postives = 567/777 (72.97%), Query Frame = 0
Query: 47 CSNSVSSASPFPGLDVETKTKTGALCFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPN 106
CS+ + SA+ LD E +T+ + FE+TKE+I+KM KVELSVSAYDT+WVAMVPSP+
Sbjct: 10 CSSPI-SATLERRLDSEVQTRANNVSFEQTKEKIRKMLEKVELSVSAYDTSWVAMVPSPS 69
Query: 107 SLNDPLFPECINWVLDNQHPDGSWGI-IHNHHLLMKASLLSTLACALTLKRWNIGHEHMS 166
S N PLFP+C+ W+LDNQH DGSWG+ H+H L K L STLA L LK+W IG ++
Sbjct: 70 SQNAPLFPQCVKWLLDNQHEDGSWGLDNHDHQSLKKDVLSSTLASILALKKWGIGERQIN 129
Query: 167 KALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDL 226
K L FI+ N TDE P GFDIIFPGMI+YA+DL+L +PL VD +++K++LDL
Sbjct: 130 KGLQFIELNSALVTDETIQKPTGFDIIFPGMIKYARDLNLTIPLGSEVVDDMIRKRDLDL 189
Query: 227 RSCRSN--SEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDG 286
+ C S S+G +AYLAYV EG L+DW+++++YQRKNGSLF+SP+TTA AF +DG
Sbjct: 190 K-CDSEKFSKGREAYLAYVLEGTRNLKDWDLIVKYQRKNGSLFDSPATTAAAFTQFGNDG 249
Query: 287 CFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQ 346
C YL SLLQKF+ +VP++YP D YARL ++ L+ LGIDR FK EI+S+LDETYR W++
Sbjct: 250 CLRYLCSLLQKFEAAVPSVYPFDQYARLSIIVTLESLGIDRDFKTEIKSILDETYRYWLR 309
Query: 347 GEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDL-FSNCLGGYLKDFGSSLELFKA 406
G+E+I LD +TCA+AFR+L GYDVS D L F+E+ FS+ L GY+K+ S LELFKA
Sbjct: 310 GDEEICLDLATCALAFRLLLAHGYDVSYDPLKPFAEESGFSDTLEGYVKNTFSVLELFKA 369
Query: 407 SQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERL 466
+Q YP ES L+ WT +L LSS S R D+ LK+E +AL F +LER
Sbjct: 370 AQ--SYPHESALKKQCCWTKQYLEMELSSWVKTSVR-DKYLKKEVEDALAFPSYASLERS 429
Query: 467 LNKRAMENYN-IDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVE 526
++R + N + ++ R++KT+Y N D L+LAV+DFN QSIH +E++ L+RW+VE
Sbjct: 430 DHRRKILNGSAVENTRVTKTSYRLHNICTSDILKLAVDDFNFCQSIHREEMERLDRWIVE 489
Query: 527 NRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELI 586
NRL +LKFARQKLAYCYFS AATL SPEL DAR+SWAK GVLTTVVDDFFDVGGS+ EL
Sbjct: 490 NRLQELKFARQKLAYCYFSGAATLFSPELSDARISWAKGGVLTTVVDDFFDVGGSKEELE 549
Query: 587 NLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALL 646
NLI LVEKWD+NG +Y S+ VEIIF L T+ E +A +QGR+V +++ IWL LL
Sbjct: 550 NLIHLVEKWDLNGVPEYSSEHVEIIFSVLRDTILETGDKAFTYQGRNVTHHIVKIWLDLL 609
Query: 647 KSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLF 706
KSM +EAEW +K P +++YMEN YISFALGPIVLPA + +GP LPE+ + S +Y++L+
Sbjct: 610 KSMLREAEWSSDKSTPSLEDYMENAYISFALGPIVLPATYLIGPPLPEKTVDSHQYNQLY 669
Query: 707 KLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELL 766
KL+ST GRLLND Q F+RES+EGKLNA+SL M + + +KE E++KG ER R EL
Sbjct: 670 KLVSTMGRLLNDIQGFKRESAEGKLNAVSLHMKHERDNRSKEVIIESMKGLAERKREELH 729
Query: 767 RLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALLEQPMVLDE 819
+LVL+EK S VPR CK+ F KMS V++LFYRKDDGFTS++LM+ K+++ +P+ L E
Sbjct: 730 KLVLEEKGSVVPRECKEAFLKMSKVLNLFYRKDDGFTSNDLMSLVKSVIYEPVSLQE 781
BLAST of Moc04g00170 vs. TAIR 10
Match:
AT1G61120.1 (terpene synthase 04 )
HSP 1 Score: 287.7 bits (735), Expect = 2.9e-77
Identity = 223/771 (28.92%), Postives = 375/771 (48.64%), Query Frame = 0
Query: 89 LSVSAYDTAWVAMVPSPNSLND----PLFPECINWVLDNQHP-DGSWGIIHNHHLLMKAS 148
+S SAYDTAW++M+ +++D P+F C++W++ NQ+ +G W ++ +
Sbjct: 36 VSPSAYDTAWLSMIEEDINVDDNELKPMFQGCLDWIMCNQNAREGFWMNSTSYTTVADGR 95
Query: 149 ----------LLSTLACALTLKRWNIGHEHMSKALNFIKSN----IVSATDEKQYSPVGF 208
L STLAC + L++WNIG H+ K +I+ N I +E+ P F
Sbjct: 96 DEDGEKDMCILTSTLACVVALQKWNIGCFHLHKGTRYIERNTEMIIGKYINEEGSYPRWF 155
Query: 209 DIIFPGMIEYAKDLDLNLPLAPANVDAL----LQKKELDLRSCRSNSEGGKAYLAYVSEG 268
I F G++E A+ L L+ + ++ + Q++E+ R + K LAY+
Sbjct: 156 AIKFTGILELAQKLGLHFVFSSRCIEMIKGMFYQRQEIIQREKLVHDCNYKPLLAYLEVL 215
Query: 269 IGRL----RDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPT 328
+L ++ +V +GSLF SPS TA AFM + C YL +L+QK N VP
Sbjct: 216 PSKLYVTNQEDIIVKSLDSMDGSLFQSPSATASAFMLTRNTKCLAYLQNLVQKCPNGVPQ 275
Query: 329 IYPL-DIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEED------IFLDAST 388
YPL + +L MV+ ++ G+ F EI VL++ Y + + + + +
Sbjct: 276 KYPLNEDLIKLSMVNLIESTGLGEFFGIEIEHVLEQVYSRYEEKDFERMPMSYLADQLHK 335
Query: 389 CAMAFRMLRLGGYDVSSDQLTQFSEDLFS-NCLGGYLKDF-GSSLELFKASQIIIYPDES 448
++AFRMLR+ G DVS F D + N L + F L +++A+ +++P E
Sbjct: 336 DSLAFRMLRMHGRDVSPRSFCWFLNDQETRNHLERNIDSFLLVILSVYRATD-LMFPGEH 395
Query: 449 ILENINSWTNSFLSHGLSSGSVHSDRPDRLLKQEAI-NALEFTYSNTLERLLNKRAMENY 508
L+ +T + L R +K++ I + L + L+ L ++ +E+
Sbjct: 396 DLQEAREYTRNLL------------EKRRSIKEKMIMHELSTPWIARLKHLDHRMWIEDK 455
Query: 509 NIDIVRISKTTYTCLNFGHQDFL-ELAVEDFNILQSIHHKELKELERWVVENRLDKLKFA 568
N +++ + K ++ L+ + D L LA +F Q+ + +EL+EL WV + L + F
Sbjct: 456 NSNVLSMEKASFLRLHSSYSDKLTHLAARNFEFQQAKYCRELEELTMWVKKWGLSDIGFG 515
Query: 569 RQKLAYCYFSAAATLT-SPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEK 628
R+K YCYF+ +L ++ +L+ AK +L T+ DDFFD GS +L L + V +
Sbjct: 516 REKTTYCYFATVTSLPYEYAIKFGKLA-AKTAILITIADDFFDEKGSFNDLEGLTKAVLR 575
Query: 629 WDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAE 688
W E + IIF AL V E ++ ++ +IW +S +EAE
Sbjct: 576 W----EGEELKSYGNIIFRALDDIVRETANTCRTHHKTDIIVHLRNIWGETFESWLREAE 635
Query: 689 WLKNKIAPPMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGR 748
W K MDEY+ NG IS A I L + P P + Y + L+ R
Sbjct: 636 WSKKGHTSSMDEYIRNGMISIAAHTIALSISCLMEPCFPHNKLKPGNYDSITTLLMIIPR 695
Query: 749 LLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKN 808
LLND QS+++E +GK+N++ L M N G E++ I+ ++ R+E L VL +
Sbjct: 696 LLNDLQSYQKEQEQGKMNSVLLHMKNHPG-LEIEDSIAHIEKIIDSKRKEFLEHVLVDGL 755
Query: 809 STVPRACKDLFWKMSCVVHLFYRKDDGFTSH-ELMNSAKALLEQPMVLDEL 820
S +P+ CK++ V +F+ K + + S+ E+++ K L P+ + EL
Sbjct: 756 SDLPKPCKEIHMSCCKVFEMFFNKKNRYDSNTEMLHDIKKALYDPINVYEL 787
BLAST of Moc04g00170 vs. TAIR 10
Match:
AT4G02780.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 272.3 bits (695), Expect = 1.3e-72
Identity = 168/521 (32.25%), Postives = 273/521 (52.40%), Query Frame = 0
Query: 73 FEETKERIKKMFNKV---ELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGS 132
F+E + +K + + E+++SAYDTAWVA++ + + P FP + W+ +NQ DGS
Sbjct: 94 FKEAVKSVKTILRNLTDGEITISAYDTAWVALIDAGD--KTPAFPSAVKWIAENQLSDGS 153
Query: 133 WG--IIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEK-QYSP 192
WG + ++H L++TLAC + L+ WN+ +K + F + NI DE ++ P
Sbjct: 154 WGDAYLFSYH----DRLINTLACVVALRSWNLFPHQCNKGITFFRENIGKLEDENDEHMP 213
Query: 193 VGFDIIFPGMIEYAKDLDLNLPLAPANVDALLQKKELDL-RSCRSNSEGGKAYLAYVSEG 252
+GF++ FP ++E A+ +++++P + + KKEL L R + L + EG
Sbjct: 214 IGFEVAFPSLLEIARGINIDVPYDSPVLKDIYAKKELKLTRIPKEIMHKIPTTLLHSLEG 273
Query: 253 IGRLRDWEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPL 312
+ L DWE +++ Q ++GS SPS+TA AFM D C YL + +++F+ VP ++P+
Sbjct: 274 MRDL-DWEKLLKLQSQDGSFLFSPSSTAFAFMQTRDSNCLEYLRNAVKRFNGGVPNVFPV 333
Query: 313 DIYARLCMVDNLQRLGIDRHFKEEIRSVLDETYR-------CWMQGEEDIFLDASTCAMA 372
D++ + +VD LQRLGI R+F+EEI+ LD +R CW + D AMA
Sbjct: 334 DLFEHIWIVDRLQRLGISRYFEEEIKECLDYVHRYWTDNGICWARCSH--VQDIDDTAMA 393
Query: 373 FRMLRLGGYDVSSDQLTQFSEDLFSNCLGGYLKDFGSSL-ELFKASQIIIYPDESILENI 432
FR+LR GY VS+D F ++ C G + + L++ASQ + +P E IL+N
Sbjct: 394 FRLLRQHGYQVSADVFKNFEKEGEFFCFVGQSNQAVTGMFNLYRASQ-LAFPREEILKNA 453
Query: 433 NSWTNSFL------SHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENY 492
++ ++L + + D P E ALE + +L R+ + ++ Y
Sbjct: 454 KEFSYNYLLEKREREELIDKWIIMKDLPG-----EIGFALEIPWYASLPRVETRFYIDQY 513
Query: 493 -NIDIVRISKTTYTCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFA 552
+ V I KT Y + +LELA +D+N Q+ H E ++W ENRL +
Sbjct: 514 GGENDVWIGKTLYRMPYVNNNGYLELAKQDYNNCQAQHQLEWDIFQKWYEENRLSEWGVR 573
Query: 553 RQKLAYCYFSAAATLTSPELRDARLSWAKNGVLTTVVDDFF 572
R +L CY+ AAAT+ E R+ WAK+ VL + F
Sbjct: 574 RSELLECYYLAAATIFESERSHERMVWAKSSVLVKAISSSF 599
BLAST of Moc04g00170 vs. TAIR 10
Match:
AT4G16740.1 (terpene synthase 03 )
HSP 1 Score: 156.4 bits (394), Expect = 1.0e-37
Identity = 140/523 (26.77%), Postives = 239/523 (45.70%), Query Frame = 0
Query: 310 RLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQG-----EEDIFLDASTCAMAFRMLRL 369
+L ++D LQRLG+ HF++EI+ L + ++ + + + D A+ FR+LR
Sbjct: 77 QLELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQ 136
Query: 370 GGYDVSSDQLTQFSEDLFSNCLGGYL--KDFGSSLELFKASQIIIYPDESILENINSWTN 429
G+ + ++D+F +G L KD L L++AS + D + E+I T
Sbjct: 137 HGFSI--------AQDVFDGNIGVDLDDKDIKGILSLYEASYLSTRIDTKLKESIYYTTK 196
Query: 430 SFLSHGLSSGSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTT 489
RL K +N E T S TL R++ Y+ + R+
Sbjct: 197 ------------------RLRKFVEVNKNE-TKSYTLRRMVIHALEMPYHRRVGRLEARW 256
Query: 490 YTCLNFGHQD----FLELAVEDFNILQSIHHKELKELERWVVENRLDK-LKFARQKLAYC 549
Y + D LELA DFN +Q+IH ELK L W + L K L F R ++
Sbjct: 257 YIEVYGERHDMNPILLELAKLDFNFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEG 316
Query: 550 YFSAAATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEAD 609
YFS+ + PE R K +L T +DD +D+ G+ EL +VEKWDVN +
Sbjct: 317 YFSSVGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKWDVN-RLE 376
Query: 610 YCSKEVEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAP 669
+++ FL L + +I L +G +V+ + + W + + KEA+W K+ P
Sbjct: 377 ELPNYMKLCFLCLVNEINQIGYFVLRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKP 436
Query: 670 PMDEYMENGYISFALGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSF 729
+EYM+NG+IS ++ P +L LF + +++GS H + + +T RL ND +
Sbjct: 437 NFEEYMQNGWISSSV-PTILLHLFCLLSDQTLDILGSYN-HSVVRSSATILRLANDLATS 496
Query: 730 ERESSEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACK 789
E + G C ++ G ++ E+ I+G + + L +++K+ + +
Sbjct: 497 SEELARGDTMKSVQCHMHETG-ASEAESRAYIQGIIGVAWDD---LNMEKKSCRLHQGFL 556
Query: 790 DLFWKMSCVVHLFYRKDDGF---TSHELMNSAKALLEQPMVLD 818
+ + V Y+ DG + +N ++LL P+ L+
Sbjct: 557 EAAANLGRVAQCVYQYGDGHGCPDKAKTVNHVRSLLVHPLPLN 565
BLAST of Moc04g00170 vs. TAIR 10
Match:
AT4G16730.1 (terpene synthase 02 )
HSP 1 Score: 150.6 bits (379), Expect = 5.5e-36
Identity = 137/500 (27.40%), Postives = 220/500 (44.00%), Query Frame = 0
Query: 310 RLCMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGE-EDIFLDASTCAMAFRMLRLGGYD 369
+L M+D+LQRLGI H+K EI +L + + GE E D ++ F +LR G+D
Sbjct: 44 QLEMIDSLQRLGISYHYKHEIHDILRKIHD--QHGEIERETQDLHATSLEFILLRQHGFD 103
Query: 370 VSSDQLTQFSEDLFSNCLGGYLK----DFGSSLELFKASQIIIYPDESILENINSWTNSF 429
VS D D+F + G + K D L L++AS + E L+ + N
Sbjct: 104 VSQDAF-----DVFISETGEFRKTLHSDIKGLLSLYEASYFSM-DSEFKLKETRIYANKR 163
Query: 430 LSHGLSSGSVHSDRPDRLLKQEAI-NALEFTYSNTLERLLNKRAMENYNIDIVRISKTTY 489
LS ++ S R D E + ALE Y ++ RL + + Y K
Sbjct: 164 LSEFVAESSKTICREDETYILEMVKRALETPYHWSIRRLEARWYINVYE------KKHEM 223
Query: 490 TCLNFGHQDFLELAVEDFNILQSIHHKELKELERWVVENRLDK-LKFARQKLAYCYFSAA 549
L LE A DFN+LQ+ H +ELK + W L K L F R ++ YF
Sbjct: 224 NPL------LLEFAAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRITESYFWTI 283
Query: 550 ATLTSPELRDARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKE 609
PE + R K +L ++DD +D+ G+ EL +VEKWDVN +
Sbjct: 284 GIFYEPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKWDVN-HVERLPNY 343
Query: 610 VEIIFLALHGTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEY 669
+ + FL L+ + +I L +G +V+ + +W L K+ E++W K P +EY
Sbjct: 344 MRMCFLFLYNEINQIGYDVLRDKGLNVIPYLKQVWTDLFKTFLTESKWYKTGHKPSFEEY 403
Query: 670 MENGYISFALGPIVLPALFFVGPKLPEEVI-GSCEYHKLFKLMSTCGRLLNDTQSFERES 729
M+NG IS ++ I+L + + ++ + + H + + +T RL ND + E
Sbjct: 404 MQNGVISSSVPTILLHLFSVLSDHISDQTLTDDSKNHSVVRSCATILRLANDLATSTEEM 463
Query: 730 SEGKLNAMSLCMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFW 789
+ G C + ++EEA ++ + + + + S++PR
Sbjct: 464 ARGDSPKSVQCYMYE-TRASEEEARRHMQSMISDSWDIINSDLKTAHTSSLPRGFLAAAA 521
Query: 790 KMSCVVHLFYRKDDGFTSHE 802
++ VV YR DG S E
Sbjct: 524 NLNRVVQCIYRHGDGHGSPE 521
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022147910.1 | 0.0e+00 | 100.00 | ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia] | [more] |
XP_022147908.1 | 0.0e+00 | 98.32 | ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia] | [more] |
XP_022147909.1 | 0.0e+00 | 98.08 | ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia] | [more] |
XP_038888071.1 | 0.0e+00 | 84.08 | ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida] >XP_03888... | [more] |
XP_038888074.1 | 0.0e+00 | 85.50 | ent-kaur-16-ene synthase, chloroplastic isoform X3 [Benincasa hispida] | [more] |
Match Name | E-value | Identity | Description | |
Q39548 | 0.0e+00 | 78.35 | Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1 | [more] |
A0A2K9RFY0 | 4.4e-256 | 55.93 | Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 ... | [more] |
A0A1Z3GC64 | 1.9e-254 | 55.33 | Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1... | [more] |
A0A0U4CDK4 | 8.6e-252 | 55.05 | Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS P... | [more] |
A0A3G1QTS7 | 2.1e-250 | 54.82 | Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1D1G1 | 0.0e+00 | 100.00 | ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=367... | [more] |
A0A6J1D2L1 | 0.0e+00 | 98.32 | ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=367... | [more] |
A0A6J1D2F0 | 0.0e+00 | 98.08 | ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=367... | [more] |
A0A1S3BG06 | 0.0e+00 | 84.22 | ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LO... | [more] |
Q9FRX5 | 0.0e+00 | 84.10 | Ent-kaurene synthase OS=Cucumis sativus OX=3659 GN=CsKS1 PE=2 SV=1 | [more] |