Moc03g30620 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g30620
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	E3 ubiquitin-protein ligase SIS3-like
Location	chr3: 21817502 .. 21820367 (-)
RNA-Seq Expression	Moc03g30620
Synteny	Moc03g30620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTATTCGAGGGGTCGATTTCAAATGGTATTCCTCTTTCTCATCGTTCTATTGGAATGTAAATTCAGCCATATTGGTGGGTTTTTGACATGGTTTGGAATTTTGGGGTTTTTCAGGTACGATGGGTTCTTCTTGTCGATGCTTGCTACTAGCATGTATCCTTTGCCATATTAGTGGCGGCTGTTCTTATTTAGGCTTGTCTTGACATTGTTATAAACTGTAATCGAATTTCATATGTCATCTTTATGCAGTTGTAGCCAGCCAAAACAAAATATTATTATGTGACAATAATCTCGTAATCATTAGATGTATGATTATTCTTTGGACAGCATTCGTCAAAACCGCAGCCTTGATTATGTCACATCTTTCTTGCTTTTCATTAACCCTGTTGTGTTTTTACATGAACTTTCTTAGCACTCGAATGATTATAGTATGGGGTAGAAGTTTCATTGACATTTTGACTATCTGAAGACTAGCAATAGCACTTTTGGCCCCTTGTGAGAATTTCATTTTATCTTTTCTTTAACTGTTTTACTTTGTAGAATAATTGTGACCATCAATTGGAAGCGATACAATACGTGTACATACCCGTTGCACATATGGATCGTGGTACGCATCCGAGTTCTAAAGAAGCGATACAAGTTTAAGATATATTTATAATTTATGATTGCTTGCTTCTGATGGCTATGTATTTTACTTTCTTCATTCAGGTTGATTATACCTCTGTGTTTGCTTTTCGCCTTTTGCTGTTCATAGATAATGCACTTGCTACTGGAATGGGATTGTATGGCCTTCTTTCTCTCTCTAAGATTTTTTCTACCTCTGAATTGTTTTTGAAGATGAGTTTCCGTTGAATGAGTTCAATTAGGACCAAATATACAACCAGTTCATTACTTCAAATCTTACTCAAAAACTATTAACAAGGTTATTTCCATATACTCACATATCACTTATGGATTTTTTTTTTCAATGAAATCATTAGAGGCACCTAAATGACTCTGCTTATGGTATATTTCTGATTCATAAGTGGTGTGAGTGCCTTATTCTTCTTTCTTACTGTTGAATTTAAATTTCATTAGGGATTTTGGATGGCAGCAGCAGTATGCTCGGTTTTGTGGCAGAGTTGTGGTTCTTTCTATTCTTTCTCTATTGCTCTACCCGGTTTTATGTGTGTGGACTGTTATTGGAACCTTGTGGTTTAAAGATGCTAAAGACTGTGTAAGCCAAAAATCTCCTCAGTTGACATTGGCCAAATCATCTGTTTACAGACCTCGATTATTTAGTTCATCTATTGTTGATGCATGCAGTTGCCAGAAGAAGGTCAAAAATGGGGTTTTCTCATTTGGTTGCTTTTTAGCTACTGTGCTCTGCTTTGCATTGCTTGCACTTCAGTGGGAAAGGTTATCCATTTTCTGTGTACTTCTCCATTCTCTTACCTATATTTCCCTTGTTTGTTAGTCGACTAAATTTTTAGCAAGTTTAGACTATTAATTGAGGCATATGCCATGTAATGTGGCAGAGCTATCCTCGCAAACTTGGTTGCTTCAAAATTTTCTTATTTCATGTTTTTCCGATTGCTCCTAATATCGTCTTTTTTGCAGTGGTTAGTGCGTAGGCAAGCGCACATGTTACGTGCCCAACAAGGCATACCCGTATCAGAATACGGGGTAAGCTCGAAAACTCCTAATACCATCTTTTTTTGTTTGTGTTCTCCTTTCTTTTTGGCTTTGTCATAAACTTTATTTCTGGTTAAAAGATAATGTAAAACTTCTTTGTTCTTGGGTTCATATGTCATTTTAGCGATTAGTATCATTTTATAACTATCAAACATTGAACTGATTAGCATTTTCTGCAAACCAGGTATTGGTTGACATGATTAGGGTTCCTGATTGGGCATTTGAAGTTGCAGGACTGGAAACACGAGGCTTTGACCAAGATTCTACTGGGTACCATCCCGGGCTTTTCTTAACCTCGGCTCAGGTAAGTACGTGTGAAGTTTAGATCAATGCATATGGAAACTAAATAACATTCATTTGAGTAATGCTGCTTCCCAATCAACTTCTATAGTCGCCATTTCGCAAACAATATGTTTAATGGATCCTGTAATGTGTTACGTACTGAAAATTGGAGAGTGAAGTGGGAGGAAATCTGGATGGAACTGCATTTACCTTATGAATGTCTACCGAAATCTATAATCGTCAATGTGAAGTTGTTCTAAGCGGGTTTGTTGGGAATTTGTGCAGAGGGAAGCTGTGGAAGCTCTTATCCAGGAACTTCCAAAGTTCAGGCTAAAGGCAGTCCCAACTGATTGCAGTGAATGCCCGATTTGCCTCGAAGAATTCCATGTCGGTAATGAGGTAAATTGTTGTCATCATTCATCTTAAACGTTACCAGTTTGCCTTTTTTTACTTCGAACTTCTATGATACAATCTTATTCATTCTGTTCAAATATAGATCGAATAATTTAGATGCAGGATGAACACTTTCTATGGCGATGGCTACTCTGTTCTGGGATTTAGTCATTATTCACTTTTTTAAACATCAAGAGTTATAGAATGAATGCAGACATATCCATTTGATTTTGGTGTTGAACTTTTGTGCAGGTTCGCGGTATGCCGTGTGCTCACAACTTCCACGTCGAATGTATCGATGAGTGGCTTCGTCTTAATGTCAAATGTCCTCGGTGCCGCTCCTCAGTCTTCCCAAATCTTGATCTCAGCGCTCTTTCTGACCTGCGTTCGGACACAGACCAAACACCCACCAATGGTTACATAACAAGGCAACCTTCAAGTCTATCGACATCCAGTCCAGTCCCTGCAGCTCATACAGTAAACACACAAGCCTCGATACCGACACTGCCATAG

mRNA sequence

ATGGCTATTCGAGGGGTCGATTTCAAATGGTACGATGGGTTCTTCTTGTCGATGCTTGCTACTAGCATAATAATTGTGACCATCAATTGGAAGCGATACAATACGTGTACATACCCGTTGCACATATGGATCGTGGTTGATTATACCTCTGTGTTTGCTTTTCGCCTTTTGCTGTTCATAGATAATGCACTTGCTACTGGAATGGGATTGGATTTTGGATGGCAGCAGCAGTATGCTCGGTTTTGTGGCAGAGTTGTGGTTCTTTCTATTCTTTCTCTATTGCTCTACCCGGTTTTATGTGTGTGGACTGTTATTGGAACCTTGTGGTTTAAAGATGCTAAAGACTGTTTGCCAGAAGAAGGTCAAAAATGGGGTTTTCTCATTTGGTTGCTTTTTAGCTACTGTGCTCTGCTTTGCATTGCTTGCACTTCAGTGGGAAAGTGGTTAGTGCGTAGGCAAGCGCACATGTTACGTGCCCAACAAGGCATACCCGTATCAGAATACGGGGTATTGGTTGACATGATTAGGGTTCCTGATTGGGCATTTGAAGTTGCAGGACTGGAAACACGAGGCTTTGACCAAGATTCTACTGGGTACCATCCCGGGCTTTTCTTAACCTCGGCTCAGAGGGAAGCTGTGGAAGCTCTTATCCAGGAACTTCCAAAGTTCAGGCTAAAGGCAGTCCCAACTGATTGCAGTGAATGCCCGATTTGCCTCGAAGAATTCCATGTCGGTAATGAGGTTCGCGGTATGCCGTGTGCTCACAACTTCCACGTCGAATGTATCGATGAGTGGCTTCGTCTTAATGTCAAATGTCCTCGGTGCCGCTCCTCAGTCTTCCCAAATCTTGATCTCAGCGCTCTTTCTGACCTGCGTTCGGACACAGACCAAACACCCACCAATGGTTACATAACAAGGCAACCTTCAAGTCTATCGACATCCAGTCCAGTCCCTGCAGCTCATACAGTAAACACACAAGCCTCGATACCGACACTGCCATAG

Coding sequence (CDS)

ATGGCTATTCGAGGGGTCGATTTCAAATGGTACGATGGGTTCTTCTTGTCGATGCTTGCTACTAGCATAATAATTGTGACCATCAATTGGAAGCGATACAATACGTGTACATACCCGTTGCACATATGGATCGTGGTTGATTATACCTCTGTGTTTGCTTTTCGCCTTTTGCTGTTCATAGATAATGCACTTGCTACTGGAATGGGATTGGATTTTGGATGGCAGCAGCAGTATGCTCGGTTTTGTGGCAGAGTTGTGGTTCTTTCTATTCTTTCTCTATTGCTCTACCCGGTTTTATGTGTGTGGACTGTTATTGGAACCTTGTGGTTTAAAGATGCTAAAGACTGTTTGCCAGAAGAAGGTCAAAAATGGGGTTTTCTCATTTGGTTGCTTTTTAGCTACTGTGCTCTGCTTTGCATTGCTTGCACTTCAGTGGGAAAGTGGTTAGTGCGTAGGCAAGCGCACATGTTACGTGCCCAACAAGGCATACCCGTATCAGAATACGGGGTATTGGTTGACATGATTAGGGTTCCTGATTGGGCATTTGAAGTTGCAGGACTGGAAACACGAGGCTTTGACCAAGATTCTACTGGGTACCATCCCGGGCTTTTCTTAACCTCGGCTCAGAGGGAAGCTGTGGAAGCTCTTATCCAGGAACTTCCAAAGTTCAGGCTAAAGGCAGTCCCAACTGATTGCAGTGAATGCCCGATTTGCCTCGAAGAATTCCATGTCGGTAATGAGGTTCGCGGTATGCCGTGTGCTCACAACTTCCACGTCGAATGTATCGATGAGTGGCTTCGTCTTAATGTCAAATGTCCTCGGTGCCGCTCCTCAGTCTTCCCAAATCTTGATCTCAGCGCTCTTTCTGACCTGCGTTCGGACACAGACCAAACACCCACCAATGGTTACATAACAAGGCAACCTTCAAGTCTATCGACATCCAGTCCAGTCCCTGCAGCTCATACAGTAAACACACAAGCCTCGATACCGACACTGCCATAG

Protein sequence

MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFIDNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEEGQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDWAFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPTNGYITRQPSSLSTSSPVPAAHTVNTQASIPTLP

Homology

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BLAST of Moc03g30620 vs. NCBI nr
Match: XP_022137581.1 (E3 ubiquitin-protein ligase SIS3-like isoform X1 [Momordica charantia] >XP_022137582.1 E3 ubiquitin-protein ligase SIS3-like isoform X1 [Momordica charantia])

HSP 1 Score: 695.3 bits (1793), Expect = 2.6e-196
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE
Sbjct: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNTQASIPTLP 334
           NGYITRQPSSLSTSSPVPAAHTVNTQASIPTLP
Sbjct: 301 NGYITRQPSSLSTSSPVPAAHTVNTQASIPTLP 333

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BLAST of Moc03g30620 vs. NCBI nr
Match: XP_004152472.1 (E3 ubiquitin-protein ligase SIS3 isoform X1 [Cucumis sativus] >KGN64305.1 hypothetical protein Csa_013147 [Cucumis sativus])

HSP 1 Score: 624.8 bits (1610), Expect = 4.3e-175
Identity = 294/325 (90.46%), Postives = 307/325 (94.46%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINW+RYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNAL+ GMGLDFGWQQ+YARFCGRVVVLSILSLLLYP LC WTVIGTLWF+DAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIAC SVGKWLVRRQAH+ RAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+E AGLETR   QD+TGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALS+L SDT+QT  
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNT 326
           N ++T QPSSLS S+P  AAHT N+
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENS 325

[Close]

BLAST of Moc03g30620 vs. NCBI nr
Match: XP_008438310.1 (PREDICTED: E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo] >TYJ99152.1 E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 622.9 bits (1605), Expect = 1.6e-174
Identity = 296/331 (89.43%), Postives = 310/331 (93.66%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINW+RYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNAL+ GMGLDFGWQQ+YARFCGRVVVLSILSLLLYP LC WTVIGTLWF+DAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIAC SVGKWL RRQAH+ RAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+E AGLETR   QD+TGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALS+L SDT+QT  
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNTQASIPT 332
           N ++T QPSSLS S+P  AAHT NT AS+ T
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENT-ASLHT 330

[Close]

BLAST of Moc03g30620 vs. NCBI nr
Match: KAA0055223.1 (E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 620.9 bits (1600), Expect = 6.3e-174
Identity = 295/331 (89.12%), Postives = 309/331 (93.35%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINW+RYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNAL+ GMGLDFGWQQ+YA FCGRVVVLSILSLLLYP LC WTVIGTLWF+DAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYAHFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIAC SVGKWL RRQAH+ RAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+E AGLETR   QD+TGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALS+L SDT+QT  
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNTQASIPT 332
           N ++T QPSSLS S+P  AAHT NT AS+ T
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENT-ASLHT 330

[Close]

BLAST of Moc03g30620 vs. NCBI nr
Match: XP_038895423.1 (E3 ubiquitin-protein ligase SIS3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 620.9 bits (1600), Expect = 6.3e-174
Identity = 290/325 (89.23%), Postives = 305/325 (93.85%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINW+RYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNAL+ GMGLDFGWQQ+YARFCGRVVVLS+LSLLLYP LC WTVIGTLWF+DAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSVLSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIAC SVGKWL RRQAH+ RAQQG+PVSEYGVL+DMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGMPVSEYGVLIDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+E AGLE RG  QD+TGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLEIRGIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALS+L SDT+QT  
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSD 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNT 326
           N ++T QPSSL  S+P  AAHT NT
Sbjct: 301 NAFVTTQPSSLLRSTPTRAAHTENT 325

[Close]

BLAST of Moc03g30620 vs. ExPASy Swiss-Prot
Match: Q8GYT9 (E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV=2)

HSP 1 Score: 506.5 bits (1303), Expect = 2.3e-142
Identity = 242/340 (71.18%), Postives = 273/340 (80.29%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MA+RGVDFKWYDGFFLSMLATS+IIV +NW RY  C YPLHIWIVVDYT+VF FR+ +F+
Sbjct: 1   MAMRGVDFKWYDGFFLSMLATSVIIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFV 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DN LA+G+GLDFG QQ+ A FCGRVVVLS+LSLLLYP L  WTVIGT WF  +K CLPEE
Sbjct: 61  DNGLASGLGLDFGSQQRNAMFCGRVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWL+FSYC LLCIA   VGKWL RRQ H+LRAQQGIP+SE+G+LVDMIRVPDW
Sbjct: 121 GQKWGFLIWLMFSYCGLLCIAFICVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AFE AG E RG  QD+  YHPGL+LT AQ EAVEALIQELPKFRLKAVP DC EC ICLE
Sbjct: 181 AFEAAGQEMRGISQDAATYHPGLYLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSD------ 300
           EFH+G+EVRG+PCAHNFHVECID+WLRLNVKCPRCR SVFP+LDLSALS+L+S       
Sbjct: 241 EFHIGHEVRGLPCAHNFHVECIDQWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHS 300

Query: 301 --TDQTPTNGYITRQPSSLSTSSPVPA-AHTVNTQASIPT 332
               +T    YI  QP S S    V +  H V+T  ++ T
Sbjct: 301 QVNTETSEARYIRSQPQSESYFLRVQSLIHPVHTDTALET 340

[Close]

BLAST of Moc03g30620 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.7e-12
Identity = 45/190 (23.68%), Postives = 83/190 (43.68%), Query Frame = 0


Query: 101 VWTVIGTLWFKDAKDCLPEEGQK--WGFLIWLLFS-YCALLCIACTSV-GKWLVRRQAHM 160
           +W +IG  W       L +E  +  W  +++L F  +  + C+A   V G  +      +
Sbjct: 226 IWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCLPCI 285

Query: 161 LRAQQGIPVSEYGVLVDMIRVPDWAFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVE-A 220
           +     +   E     D+ ++  + F   G   +  + ++ G   G+         +E  
Sbjct: 286 IAVLYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGTDSPIEHT 345

Query: 221 LIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRC 280
           L+QE             +EC ICL  +  G E+R +PC H+FH  C+D+WL +N  CP C
Sbjct: 346 LLQE------------DAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLC 403

Query: 281 RSSVFPNLDL 286
           + ++  + +L
Sbjct: 406 KYNILKSSNL 403

[Close]

BLAST of Moc03g30620 vs. ExPASy Swiss-Prot
Match: Q9FKX5 (NEP1-interacting protein-like 1 OS=Arabidopsis thaliana OX=3702 GN=ATL27 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.1e-11
Identity = 29/78 (37.18%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 200 HPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMP-CAHNFH 259
           H  +F T+  +      +  +PK R+     +   C +CL++F VG  VR +P C H FH
Sbjct: 141 HTDIFDTAISKGLTGDSLNRIPKVRITDTSPEIVSCSVCLQDFQVGETVRSLPHCHHMFH 200

Query: 260 VECIDEWLRLNVKCPRCR 277
           + CID+WLR +  CP CR
Sbjct: 201 LPCIDKWLRRHASCPLCR 218

[Close]

BLAST of Moc03g30620 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.0e-11
Identity = 34/107 (31.78%), Postives = 53/107 (49.53%), Query Frame = 0


Query: 203 LFLTSAQREAVEALIQELPKFRL-------------KAVPTDC--------------SEC 262
           L+  + Q  A +  I ++PKFR              + + T+C              +EC
Sbjct: 279 LYAVADQEGASKNDIDQMPKFRFTKTGNVEKLSGKARGIMTECGTDSPIERSLSPEDAEC 338

Query: 263 PICLEEFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPN 283
            ICL E+  G E+R +PC H+FH  CID+WL +N +CP C+ ++  N
Sbjct: 339 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNILKN 385

[Close]

BLAST of Moc03g30620 vs. ExPASy Swiss-Prot
Match: Q9ZV53 (Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.0e-10
Identity = 36/91 (39.56%), Postives = 51/91 (56.04%), Query Frame = 0


Query: 214 EALIQELPKFRLKAV---PTDCSECPICLEEFHVGNEVRGMP-CAHNFHVECIDEWLRLN 273
           ++LI  LP F  K++        +CP+CL EF   +++R +P C+H FHVECID WL  +
Sbjct: 102 QSLIDTLPVFHYKSIVGLKISPFDCPVCLCEFETEDKLRLLPKCSHAFHVECIDTWLLSH 161

Query: 274 VKCPRCRSSVFP------NLDLSALSDLRSD 295
             CP CRS++        NL  S L  L S+
Sbjct: 162 STCPLCRSNLLSGFSSHHNLSSSYLLVLESE 192

[Close]

BLAST of Moc03g30620 vs. ExPASy TrEMBL
Match: A0A6J1C8N5 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008996 PE=4 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.3e-196
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE
Sbjct: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNTQASIPTLP 334
           NGYITRQPSSLSTSSPVPAAHTVNTQASIPTLP
Sbjct: 301 NGYITRQPSSLSTSSPVPAAHTVNTQASIPTLP 333

[Close]

BLAST of Moc03g30620 vs. ExPASy TrEMBL
Match: A0A0A0LQW0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G046030 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.1e-175
Identity = 294/325 (90.46%), Postives = 307/325 (94.46%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINW+RYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNAL+ GMGLDFGWQQ+YARFCGRVVVLSILSLLLYP LC WTVIGTLWF+DAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIAC SVGKWLVRRQAH+ RAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+E AGLETR   QD+TGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALS+L SDT+QT  
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNT 326
           N ++T QPSSLS S+P  AAHT N+
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENS 325

[Close]

BLAST of Moc03g30620 vs. ExPASy TrEMBL
Match: A0A5D3BHC8 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003600 PE=4 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 8.0e-175
Identity = 296/331 (89.43%), Postives = 310/331 (93.66%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINW+RYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNAL+ GMGLDFGWQQ+YARFCGRVVVLSILSLLLYP LC WTVIGTLWF+DAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIAC SVGKWL RRQAH+ RAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+E AGLETR   QD+TGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALS+L SDT+QT  
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNTQASIPT 332
           N ++T QPSSLS S+P  AAHT NT AS+ T
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENT-ASLHT 330

[Close]

BLAST of Moc03g30620 vs. ExPASy TrEMBL
Match: A0A1S3AVR9 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483452 PE=4 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 8.0e-175
Identity = 296/331 (89.43%), Postives = 310/331 (93.66%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINW+RYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNAL+ GMGLDFGWQQ+YARFCGRVVVLSILSLLLYP LC WTVIGTLWF+DAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIAC SVGKWL RRQAH+ RAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+E AGLETR   QD+TGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALS+L SDT+QT  
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNTQASIPT 332
           N ++T QPSSLS S+P  AAHT NT AS+ T
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENT-ASLHT 330

[Close]

BLAST of Moc03g30620 vs. ExPASy TrEMBL
Match: A0A5A7UGY5 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00650 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.0e-174
Identity = 295/331 (89.12%), Postives = 309/331 (93.35%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINW+RYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DNAL+ GMGLDFGWQQ+YA FCGRVVVLSILSLLLYP LC WTVIGTLWF+DAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYAHFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIAC SVGKWL RRQAH+ RAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+E AGLETR   QD+TGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSDTDQTPT 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALS+L SDT+QT  
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NGYITRQPSSLSTSSPVPAAHTVNTQASIPT 332
           N ++T QPSSLS S+P  AAHT NT AS+ T
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENT-ASLHT 330

[Close]

BLAST of Moc03g30620 vs. TAIR 10
Match: AT3G47990.1 (SUGAR-INSENSITIVE 3 )

HSP 1 Score: 506.5 bits (1303), Expect = 1.6e-143
Identity = 242/340 (71.18%), Postives = 273/340 (80.29%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWKRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MA+RGVDFKWYDGFFLSMLATS+IIV +NW RY  C YPLHIWIVVDYT+VF FR+ +F+
Sbjct: 1   MAMRGVDFKWYDGFFLSMLATSVIIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFV 60

Query: 61  DNALATGMGLDFGWQQQYARFCGRVVVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEE 120
           DN LA+G+GLDFG QQ+ A FCGRVVVLS+LSLLLYP L  WTVIGT WF  +K CLPEE
Sbjct: 61  DNGLASGLGLDFGSQQRNAMFCGRVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACTSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWL+FSYC LLCIA   VGKWL RRQ H+LRAQQGIP+SE+G+LVDMIRVPDW
Sbjct: 121 GQKWGFLIWLMFSYCGLLCIAFICVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDW 180

Query: 181 AFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AFE AG E RG  QD+  YHPGL+LT AQ EAVEALIQELPKFRLKAVP DC EC ICLE
Sbjct: 181 AFEAAGQEMRGISQDAATYHPGLYLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSDLRSD------ 300
           EFH+G+EVRG+PCAHNFHVECID+WLRLNVKCPRCR SVFP+LDLSALS+L+S       
Sbjct: 241 EFHIGHEVRGLPCAHNFHVECIDQWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHS 300

Query: 301 --TDQTPTNGYITRQPSSLSTSSPVPA-AHTVNTQASIPT 332
               +T    YI  QP S S    V +  H V+T  ++ T
Sbjct: 301 QVNTETSEARYIRSQPQSESYFLRVQSLIHPVHTDTALET 340

[Close]

BLAST of Moc03g30620 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.2e-13
Identity = 45/190 (23.68%), Postives = 83/190 (43.68%), Query Frame = 0


Query: 101 VWTVIGTLWFKDAKDCLPEEGQK--WGFLIWLLFS-YCALLCIACTSV-GKWLVRRQAHM 160
           +W +IG  W       L +E  +  W  +++L F  +  + C+A   V G  +      +
Sbjct: 226 IWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCLPCI 285

Query: 161 LRAQQGIPVSEYGVLVDMIRVPDWAFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVE-A 220
           +     +   E     D+ ++  + F   G   +  + ++ G   G+         +E  
Sbjct: 286 IAVLYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGTDSPIEHT 345

Query: 221 LIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRC 280
           L+QE             +EC ICL  +  G E+R +PC H+FH  C+D+WL +N  CP C
Sbjct: 346 LLQE------------DAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLC 403

Query: 281 RSSVFPNLDL 286
           + ++  + +L
Sbjct: 406 KYNILKSSNL 403

[Close]

BLAST of Moc03g30620 vs. TAIR 10
Match: AT1G12760.2 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.2e-13
Identity = 45/190 (23.68%), Postives = 83/190 (43.68%), Query Frame = 0


Query: 101 VWTVIGTLWFKDAKDCLPEEGQK--WGFLIWLLFS-YCALLCIACTSV-GKWLVRRQAHM 160
           +W +IG  W       L +E  +  W  +++L F  +  + C+A   V G  +      +
Sbjct: 155 IWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCLPCI 214

Query: 161 LRAQQGIPVSEYGVLVDMIRVPDWAFEVAGLETRGFDQDSTGYHPGLFLTSAQREAVE-A 220
           +     +   E     D+ ++  + F   G   +  + ++ G   G+         +E  
Sbjct: 215 IAVLYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGTDSPIEHT 274

Query: 221 LIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRC 280
           L+QE             +EC ICL  +  G E+R +PC H+FH  C+D+WL +N  CP C
Sbjct: 275 LLQE------------DAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLC 332

Query: 281 RSSVFPNLDL 286
           + ++  + +L
Sbjct: 335 KYNILKSSNL 332

[Close]

BLAST of Moc03g30620 vs. TAIR 10
Match: AT4G32600.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 3.5e-13
Identity = 53/212 (25.00%), Postives = 91/212 (42.92%), Query Frame = 0


Query: 86  VVLSILSLLLYPVLCVWTVIGTLWFKDAKDCLPEEGQKWGF-LIWLLFSYC--ALLCIAC 145
           V++    + L     VW V+G +W         E    +   L++L FS    A+  I C
Sbjct: 226 VIVEYFKMALDCFFAVWFVVGNVWIFGGHSSAAEAPNLYRLCLVFLTFSCIGYAMPFILC 285

Query: 146 TSVGKWLVRRQAHMLRAQQGIPVSEYGVLVDMIRVPDWAFEVAGLETRGFDQDSTGYHPG 205
           T++   L      +L  ++ +          +  +P   F++    + G D  S+    G
Sbjct: 286 TTICCCL-PCIISILGYREDLTQPRGATPESINALPTHKFKLKKSRSNGDDNGSSTSEGG 345

Query: 206 LFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMPCAHNFHVECI 265
           +       E              +A+  + + C ICL ++    E+R +PC+H FH EC+
Sbjct: 346 VVAAGTDNE--------------RAISGEDAVCCICLAKYANNEELRELPCSHFFHKECV 405

Query: 266 DEWLRLNVKCPRCRSSV-FPNLDLSALSDLRS 294
           D+WL++N  CP C+S V   N DL++   L S
Sbjct: 406 DKWLKINASCPLCKSEVGEKNSDLTSQGILTS 422

[Close]

BLAST of Moc03g30620 vs. TAIR 10
Match: AT5G66070.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 7.7e-13
Identity = 29/78 (37.18%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 200 HPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMP-CAHNFH 259
           H  +F T+  +      +  +PK R+     +   C +CL++F VG  VR +P C H FH
Sbjct: 141 HTDIFDTAISKGLTGDSLNRIPKVRITDTSPEIVSCSVCLQDFQVGETVRSLPHCHHMFH 200

Query: 260 VECIDEWLRLNVKCPRCR 277
           + CID+WLR +  CP CR
Sbjct: 201 LPCIDKWLRRHASCPLCR 218

The following BLAST results are available for this feature:

BLAST of Moc03g30620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137581.1	2.6e-196	100.00	E3 ubiquitin-protein ligase SIS3-like isoform X1 [Momordica charantia] >XP_02213...	[more]
XP_004152472.1	4.3e-175	90.46	E3 ubiquitin-protein ligase SIS3 isoform X1 [Cucumis sativus] >KGN64305.1 hypoth...	[more]
XP_008438310.1	1.6e-174	89.43	PREDICTED: E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo] >TYJ9...	[more]
KAA0055223.1	6.3e-174	89.12	E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo var. makuwa]	[more]
XP_038895423.1	6.3e-174	89.23	E3 ubiquitin-protein ligase SIS3-like isoform X1 [Benincasa hispida]	[more]

BLAST of Moc03g30620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYT9	2.3e-142	71.18	E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV...	[more]
Q9LN71	1.7e-12	23.68	E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127...	[more]
Q9FKX5	1.1e-11	37.18	NEP1-interacting protein-like 1 OS=Arabidopsis thaliana OX=3702 GN=ATL27 PE=2 SV...	[more]
Q93Z92	7.0e-11	31.78	E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116...	[more]
Q9ZV53	6.0e-10	39.56	Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 P...	[more]

BLAST of Moc03g30620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8N5	1.3e-196	100.00	E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Momordica charantia OX=3673 ...	[more]
A0A0A0LQW0	2.1e-175	90.46	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G046030 P...	[more]
A0A5D3BHC8	8.0e-175	89.43	E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3AVR9	8.0e-175	89.43	E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A5A7UGY5	3.0e-174	89.12	E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo var. makuwa OX=...	[more]

BLAST of Moc03g30620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47990.1	1.6e-143	71.18	SUGAR-INSENSITIVE 3	[more]
AT1G12760.1	1.2e-13	23.68	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT1G12760.2	1.2e-13	23.68	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT4G32600.1	3.5e-13	25.00	RING/U-box superfamily protein	[more]
AT5G66070.1	7.7e-13	37.18	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 235..275 e-value: 1.1E-6 score: 38.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 234..276 e-value: 1.6E-13 score: 50.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 235..276 score: 12.769585
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 204..281 e-value: 7.8E-20 score: 72.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 291..333
None	No IPR available	PANTHER	PTHR47179:SF1	E3 UBIQUITIN-PROTEIN LIGASE SIS3	coord: 1..311
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 216..283
IPR044793	E3 ubiquitin-protein ligase SIS3	PANTHER	PTHR47179	E3 UBIQUITIN-PROTEIN LIGASE SIS3	coord: 1..311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g30620.1	Moc03g30620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0010182	sugar mediated signaling pathway
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004842	ubiquitin-protein transferase activity